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    Echelon Biosciences sphingosine kinase activity assay kit
    Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the <t>Sphingosine</t> <t>Kinase</t> <t>Activity</t> <t>Assay</t> (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).
    Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
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    sphingosine kinase activity assay kit - by Bioz Stars, 2022-12
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    Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the Sphingosine Kinase Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).

    Journal: International Journal of Molecular Sciences

    Article Title: Development of 1,2,3-Triazole-Based Sphingosine Kinase Inhibitors and Their Evaluation as Antiproliferative Agents

    doi: 10.3390/ijms18112332

    Figure Lengend Snippet: Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the Sphingosine Kinase Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).

    Article Snippet: Sphingosine Kinase Activity Assay The capability of the tested compounds to inhibit SphK1 and SphK2 enzyme activity was measured by using the Sphingosine Kinase Activity Assay (K-3500, Echelon Biosciences, Salt Lake City, UT, USA).

    Techniques: Kinase Assay, Enzyme Inhibition Assay

    Impact of tamoxifen and LCL204 on AC and Sphk1 expression and Sphk1 activity in AML cell lines. (A) Tamoxifen dose-response in KG-1 cells. KG-1 cells were seeded in 6-well plates, 5 × 10 6 /well, in media containing 5% FBS. After 60 min, cells were

    Journal: Biochimica et biophysica acta

    Article Title: Modification of sphingolipid metabolism by tamoxifen and N-desmethyltamoxifen in acute myelogenous leukemia – Impact on enzyme activity and response to cytotoxics

    doi: 10.1016/j.bbalip.2015.03.001

    Figure Lengend Snippet: Impact of tamoxifen and LCL204 on AC and Sphk1 expression and Sphk1 activity in AML cell lines. (A) Tamoxifen dose-response in KG-1 cells. KG-1 cells were seeded in 6-well plates, 5 × 10 6 /well, in media containing 5% FBS. After 60 min, cells were

    Article Snippet: Sphk1 activity was quantified by using a commercial Sphingosine Kinase Activity Assay Kit (Echelon Biosciences, Salt Lake City, UT) as the manufacturer instructed.

    Techniques: Expressing, Activity Assay