Journal: International Journal of Molecular Sciences
Article Title: Development of 1,2,3-Triazole-Based Sphingosine Kinase Inhibitors and Their Evaluation as Antiproliferative Agents
Figure Lengend Snippet: Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the Sphingosine Kinase Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).
Article Snippet: Sphingosine Kinase Activity Assay The capability of the tested compounds to inhibit SphK1 and SphK2 enzyme activity was measured by using the Sphingosine Kinase Activity Assay (K-3500, Echelon Biosciences, Salt Lake City, UT, USA).
Techniques: Kinase Assay, Enzyme Inhibition Assay