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  • 94
    Echelon Biosciences k 3000 kit
    K 3000 Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k 3000 kit/product/Echelon Biosciences
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    86
    Echelon Biosciences pi3k activity
    <t>PI3K</t> activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by <t>PI3-Kinase</t> Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)
    Pi3k Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi3k activity/product/Echelon Biosciences
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    86
    Echelon Biosciences class 3 pi3 kinase kit
    <t>PI3K</t> activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by <t>PI3-Kinase</t> Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)
    Class 3 Pi3 Kinase Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/class 3 pi3 kinase kit/product/Echelon Biosciences
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    86
    Echelon Biosciences pi3k δ
    <t>PI3K</t> activity and expression of <t>PI3K-δ</t> in ATL cells. a The activity of PI3K was quantified by <t>PI3-Kinase</t> Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)
    Pi3k δ, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences pi3k elisa kit
    Interaction between the NYAPs and the <t>PI3K</t> p85. (A–C) Tyrosine phosphorylation-dependent interaction of the NYAPs with the PI3K p85α subunit. HEK293T cells were transfected with Fyn, PI3K p85α, and FLAG-NYAP1 (A), FLAG–NYAP2 (B), FLAG–MYO16/NYAP3 (C), or their YF mutants, as indicated. The cell lysates were immunoprecipitated with the anti-FLAG antibody, immunoblotted with an anti-PI3K p85α antibody, and examined for co-precipitation of PI3K p85α with the NYAPs. The asterisk (*) in (C) represents crossreactive bands, which are faintly apparent in (A) and (B). (D) Interaction between the NYAPs and the PI3K p85 in the brain. WT and Nyap1, 2, and 3 KO brains were immunoprecipitated with anti-NYAP1, 2, or 3 antibodies and immunoblotted with an anti-PI3K p85α antibody. Nyap1, 2, and 3 KO brains were used as negative controls. (E) Phosphoproteins associated with PI3K p85α in the brain. The PI3K p85α immunoprecipitates from WT, Nyap1, 2, 3 KO, and TKO brains were immunoblotted with an anti-phospho(Tyr) p85 PI3K binding YxxM motif antibody (pYxxM). The similar results were obtained when another anti-PI3K p85α antibody (from Millipore) and the 4G10 anti-phosphotyrosine antibody were used. (F) Developmental changes in tyrosine phosphorylated binding partners of PI3K p85α. The brain lysates used in Figure 3C were immunoprecipitated with an anti-PI3K p85α antibody and immunoblotted with the 4G10 anti-phosphotyrosine, anti-PI3K p85α, and anti-p110α antibodies. The bottom panel showing the amounts of βIII-tubulin is the same as that shown in Figure 3C.
    Pi3k Elisa Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi3k elisa kit/product/Echelon Biosciences
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    Image Search Results


    PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)

    Journal: Biomarker Research

    Article Title: Phosphatidylinositol 3-kinase-δ (PI3K-δ) is a potential therapeutic target in adult T-cell leukemia-lymphoma

    doi: 10.1186/s40364-018-0138-7

    Figure Lengend Snippet: PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)

    Article Snippet: Fig. 1 PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon).

    Techniques: Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Isolation, Western Blot

    PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)

    Journal: Biomarker Research

    Article Title: Phosphatidylinositol 3-kinase-δ (PI3K-δ) is a potential therapeutic target in adult T-cell leukemia-lymphoma

    doi: 10.1186/s40364-018-0138-7

    Figure Lengend Snippet: PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)

    Article Snippet: Fig. 1 PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon).

    Techniques: Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Isolation, Western Blot

    Interaction between the NYAPs and the PI3K p85. (A–C) Tyrosine phosphorylation-dependent interaction of the NYAPs with the PI3K p85α subunit. HEK293T cells were transfected with Fyn, PI3K p85α, and FLAG-NYAP1 (A), FLAG–NYAP2 (B), FLAG–MYO16/NYAP3 (C), or their YF mutants, as indicated. The cell lysates were immunoprecipitated with the anti-FLAG antibody, immunoblotted with an anti-PI3K p85α antibody, and examined for co-precipitation of PI3K p85α with the NYAPs. The asterisk (*) in (C) represents crossreactive bands, which are faintly apparent in (A) and (B). (D) Interaction between the NYAPs and the PI3K p85 in the brain. WT and Nyap1, 2, and 3 KO brains were immunoprecipitated with anti-NYAP1, 2, or 3 antibodies and immunoblotted with an anti-PI3K p85α antibody. Nyap1, 2, and 3 KO brains were used as negative controls. (E) Phosphoproteins associated with PI3K p85α in the brain. The PI3K p85α immunoprecipitates from WT, Nyap1, 2, 3 KO, and TKO brains were immunoblotted with an anti-phospho(Tyr) p85 PI3K binding YxxM motif antibody (pYxxM). The similar results were obtained when another anti-PI3K p85α antibody (from Millipore) and the 4G10 anti-phosphotyrosine antibody were used. (F) Developmental changes in tyrosine phosphorylated binding partners of PI3K p85α. The brain lysates used in Figure 3C were immunoprecipitated with an anti-PI3K p85α antibody and immunoblotted with the 4G10 anti-phosphotyrosine, anti-PI3K p85α, and anti-p110α antibodies. The bottom panel showing the amounts of βIII-tubulin is the same as that shown in Figure 3C.

    Journal: The EMBO Journal

    Article Title: NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons

    doi: 10.1038/emboj.2011.348

    Figure Lengend Snippet: Interaction between the NYAPs and the PI3K p85. (A–C) Tyrosine phosphorylation-dependent interaction of the NYAPs with the PI3K p85α subunit. HEK293T cells were transfected with Fyn, PI3K p85α, and FLAG-NYAP1 (A), FLAG–NYAP2 (B), FLAG–MYO16/NYAP3 (C), or their YF mutants, as indicated. The cell lysates were immunoprecipitated with the anti-FLAG antibody, immunoblotted with an anti-PI3K p85α antibody, and examined for co-precipitation of PI3K p85α with the NYAPs. The asterisk (*) in (C) represents crossreactive bands, which are faintly apparent in (A) and (B). (D) Interaction between the NYAPs and the PI3K p85 in the brain. WT and Nyap1, 2, and 3 KO brains were immunoprecipitated with anti-NYAP1, 2, or 3 antibodies and immunoblotted with an anti-PI3K p85α antibody. Nyap1, 2, and 3 KO brains were used as negative controls. (E) Phosphoproteins associated with PI3K p85α in the brain. The PI3K p85α immunoprecipitates from WT, Nyap1, 2, 3 KO, and TKO brains were immunoblotted with an anti-phospho(Tyr) p85 PI3K binding YxxM motif antibody (pYxxM). The similar results were obtained when another anti-PI3K p85α antibody (from Millipore) and the 4G10 anti-phosphotyrosine antibody were used. (F) Developmental changes in tyrosine phosphorylated binding partners of PI3K p85α. The brain lysates used in Figure 3C were immunoprecipitated with an anti-PI3K p85α antibody and immunoblotted with the 4G10 anti-phosphotyrosine, anti-PI3K p85α, and anti-p110α antibodies. The bottom panel showing the amounts of βIII-tubulin is the same as that shown in Figure 3C.

    Article Snippet: PI3K was immunoprecipitated from equal amounts of total lysates obtained from WT and TKO P1 mouse brains with an anti-PI3K p85α antibody, incubated with its substrate phosphatidylinositol and ATP, and subjected to analysis with a PI3K ELISA kit (Echelon).

    Techniques: Transfection, Immunoprecipitation, Binding Assay

    Activation of the PI3K pathway by the NYAPs. (A) Membrane localization of PI3K p85α and WAVE1 in WT and TKO P1 mouse brains. WT, n=4; TKO, n=4. (B) PI3K activity in WT and TKO P1 mouse brains. PI3K was immunoprecipitated from equal amounts of total lysates obtained from WT and TKO P1 mouse brains with an anti-PI3K p85α antibody, incubated with its substrate phosphatidylinositol and ATP, and subjected to analysis with a PI3K ELISA kit (Echelon). WT, n=6; TKO, n=6. (C) Akt activity in WT and TKO P1 mouse brains. Akt activity was measured in total lysates of WT and TKO brains using an anti-phospho Ser473 Akt antibody. WT, n=3; TKO, n=3. (D) Levels of GTP-bound Rac1 in WT and TKO P1 mouse brains. Due to the low sensitivity of the assay, we pooled four P1 mouse brains per sample. WT, n=3; TKO, n=3. (E) Activation of Akt by phosphorylated NYAPs. Cultured cortical neurons were infected with recombinant adenoviruses expressing EGFP, myc-tagged wild-type NYAP1 and NYAP2, or their YF mutants. Akt activity was measured as in (C). The adenovirus expression system, instead of the sindbisvirus system, was used because of its high expression level. MYO16/NYAP3 was too large to be expressed with the adenovirus system. (F) Contactin5-mediated Akt activation in WT and TKO neurons. Cortical neurons were stimulated with 6 μg/ml C5Fc for the indicated time periods at DIV 2. Akt activation was examined as in (C). A single asterisk (*) and double asterisk (**) indicate P<0.05 and P<0.01 compared with WT, respectively.

    Journal: The EMBO Journal

    Article Title: NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons

    doi: 10.1038/emboj.2011.348

    Figure Lengend Snippet: Activation of the PI3K pathway by the NYAPs. (A) Membrane localization of PI3K p85α and WAVE1 in WT and TKO P1 mouse brains. WT, n=4; TKO, n=4. (B) PI3K activity in WT and TKO P1 mouse brains. PI3K was immunoprecipitated from equal amounts of total lysates obtained from WT and TKO P1 mouse brains with an anti-PI3K p85α antibody, incubated with its substrate phosphatidylinositol and ATP, and subjected to analysis with a PI3K ELISA kit (Echelon). WT, n=6; TKO, n=6. (C) Akt activity in WT and TKO P1 mouse brains. Akt activity was measured in total lysates of WT and TKO brains using an anti-phospho Ser473 Akt antibody. WT, n=3; TKO, n=3. (D) Levels of GTP-bound Rac1 in WT and TKO P1 mouse brains. Due to the low sensitivity of the assay, we pooled four P1 mouse brains per sample. WT, n=3; TKO, n=3. (E) Activation of Akt by phosphorylated NYAPs. Cultured cortical neurons were infected with recombinant adenoviruses expressing EGFP, myc-tagged wild-type NYAP1 and NYAP2, or their YF mutants. Akt activity was measured as in (C). The adenovirus expression system, instead of the sindbisvirus system, was used because of its high expression level. MYO16/NYAP3 was too large to be expressed with the adenovirus system. (F) Contactin5-mediated Akt activation in WT and TKO neurons. Cortical neurons were stimulated with 6 μg/ml C5Fc for the indicated time periods at DIV 2. Akt activation was examined as in (C). A single asterisk (*) and double asterisk (**) indicate P<0.05 and P<0.01 compared with WT, respectively.

    Article Snippet: PI3K was immunoprecipitated from equal amounts of total lysates obtained from WT and TKO P1 mouse brains with an anti-PI3K p85α antibody, incubated with its substrate phosphatidylinositol and ATP, and subjected to analysis with a PI3K ELISA kit (Echelon).

    Techniques: Activation Assay, Activity Assay, Immunoprecipitation, Incubation, Enzyme-linked Immunosorbent Assay, Cell Culture, Infection, Recombinant, Expressing

    The PI3K–WAVE1 association bridged by the NYAPs. (A) The neuron-specific and NYAPs-dependent association between PI3K and WAVE1. WT and TKO brains, WT livers, HEK293T cells, and CG4 cells were lysed and immunoprecipitated with an anti-WAVE1 antibody. PI3K p85α was detected only in the immunoprecipitates from WT brains, in which the NYAPs were expressed. WT livers, which do not express WAVE1, were examined as a negative control. (B) Association of Nap1 and Sra1 in the PI3K p85α immunoprecipitates. WT and TKO brains were immunoprecipitated with an anti-PI3K p85α antibody and immunoblotted with anti-Nap1 and anti-Sra1 antibodies. (C) Involvement of all members of the NYAP family in the PI3K–WAVE1 association in the brain. WT, Nyap1, 2, 3 KO, and TKO brains were immunoprecipitated with an anti-WAVE1 antibody and analysed as in (A). The PI3K–WAVE1 association persisted in NYAPs single KO mouse brains. (D) Association between PI3K and WAVE1 in HEK293T cells expressing the NYAPs. HEK293T cells were transfected with FLAG–NYAP1 and PI3K p85α and tested for the PI3K–WAVE1 association as in (A). (E) Models depicting the roles of the NYAPs. The NYAPs are involved in the activation of PI3K and the recruitment of the WAVE1 complex in neurons. The neuronal receptor(s) that mediates Contactin stimulation is currently unknown.

    Journal: The EMBO Journal

    Article Title: NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons

    doi: 10.1038/emboj.2011.348

    Figure Lengend Snippet: The PI3K–WAVE1 association bridged by the NYAPs. (A) The neuron-specific and NYAPs-dependent association between PI3K and WAVE1. WT and TKO brains, WT livers, HEK293T cells, and CG4 cells were lysed and immunoprecipitated with an anti-WAVE1 antibody. PI3K p85α was detected only in the immunoprecipitates from WT brains, in which the NYAPs were expressed. WT livers, which do not express WAVE1, were examined as a negative control. (B) Association of Nap1 and Sra1 in the PI3K p85α immunoprecipitates. WT and TKO brains were immunoprecipitated with an anti-PI3K p85α antibody and immunoblotted with anti-Nap1 and anti-Sra1 antibodies. (C) Involvement of all members of the NYAP family in the PI3K–WAVE1 association in the brain. WT, Nyap1, 2, 3 KO, and TKO brains were immunoprecipitated with an anti-WAVE1 antibody and analysed as in (A). The PI3K–WAVE1 association persisted in NYAPs single KO mouse brains. (D) Association between PI3K and WAVE1 in HEK293T cells expressing the NYAPs. HEK293T cells were transfected with FLAG–NYAP1 and PI3K p85α and tested for the PI3K–WAVE1 association as in (A). (E) Models depicting the roles of the NYAPs. The NYAPs are involved in the activation of PI3K and the recruitment of the WAVE1 complex in neurons. The neuronal receptor(s) that mediates Contactin stimulation is currently unknown.

    Article Snippet: PI3K was immunoprecipitated from equal amounts of total lysates obtained from WT and TKO P1 mouse brains with an anti-PI3K p85α antibody, incubated with its substrate phosphatidylinositol and ATP, and subjected to analysis with a PI3K ELISA kit (Echelon).

    Techniques: Immunoprecipitation, Negative Control, Expressing, Transfection, Activation Assay

    NYAPs-mediated remodelling of the actin cytoskeleton. (A–G) Requirement of PI3K- and WAVE1-interacting regions in the NYAPs for remodelling of the actin cytoskeleton in HeLa cells. HeLa cells were transfected with GST (A) or various mutants of GST-tagged NYAPs (B–G) as indicated. Cells were fixed and stained with an anti-GST antibody (green) and phalloidin-rhodamine (red). Actin stress fibres are indicated by white arrowheads. Cytosolic accumulation of collapsed actin fibres is indicated by black arrowheads. Scale bar: 50 μm. See also Supplementary Figure S10.

    Journal: The EMBO Journal

    Article Title: NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons

    doi: 10.1038/emboj.2011.348

    Figure Lengend Snippet: NYAPs-mediated remodelling of the actin cytoskeleton. (A–G) Requirement of PI3K- and WAVE1-interacting regions in the NYAPs for remodelling of the actin cytoskeleton in HeLa cells. HeLa cells were transfected with GST (A) or various mutants of GST-tagged NYAPs (B–G) as indicated. Cells were fixed and stained with an anti-GST antibody (green) and phalloidin-rhodamine (red). Actin stress fibres are indicated by white arrowheads. Cytosolic accumulation of collapsed actin fibres is indicated by black arrowheads. Scale bar: 50 μm. See also Supplementary Figure S10.

    Article Snippet: PI3K was immunoprecipitated from equal amounts of total lysates obtained from WT and TKO P1 mouse brains with an anti-PI3K p85α antibody, incubated with its substrate phosphatidylinositol and ATP, and subjected to analysis with a PI3K ELISA kit (Echelon).

    Techniques: Transfection, Staining