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  • 99
    Worthington Biochemical collagenase
    Collagenase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 8409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    ATCC m pneumoniae m129
    M Pneumoniae M129, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen long range pcr kit
    Long Range Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher genescan analysis system
    Genescan Analysis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen genomic dna
    Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 127055 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Abcam anti collagen iv
    Anti Collagen Iv, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Qiagen ld pcr reaction master mix
    Ld Pcr Reaction Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher genetic analyzer
    Genetic Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Beckman Coulter ampligrid slide
    Ampligrid Slide, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biocare Medical ccnd1
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Ccnd1, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Interactive Biosoftware alamut v 2 software
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Alamut V 2 Software, supplied by Interactive Biosoftware, used in various techniques. Bioz Stars score: 88/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Beckman Coulter sw41ti rotor beckman coulter
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Sw41ti Rotor Beckman Coulter, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Heska fluid pump
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Fluid Pump, supplied by Heska, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Teledyne Technologies fraction collector
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Fraction Collector, supplied by Teledyne Technologies, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Abcam pdh e1a
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Pdh E1a, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare de81 paper
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    De81 Paper, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen qiaspin pcr purification kit
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Qiaspin Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Medtronic aortic valve replacement
    Level of pEGFR, EGFR and <t>CCND1</t> protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.
    Aortic Valve Replacement, supplied by Medtronic, used in various techniques. Bioz Stars score: 91/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson hif1α
    HK2 regulates GBM growth and invasion in an orthotopic xenograft model. (A) Survival curve of mice injected intracranially with 2 × 10 5 of U87 cells expressing HK2shRNA-RFP ( n = 10), U87 control cells ( n = 10), U87 cells overexpressing HK2-GFP ( n = 10), or a mix of U87 cells expressing HK2-GFP and HK2shRNA-RFP ( n = 6). (B) Hematoxylin and eosin stain of tumors described in A. Bars, 2,000 µm. Arrow is pointing to the small tumor in HK2shRNA-RFP tumors. (C) Hematoxylin and eosin micrographs depicting invasiveness of HK2shRNA tumors. The arrow is pointing at the invading tumor cells within the cerebellum. Bars: (top) 250 µm; (bottom) 100 µm. Bottom graph depicts the extent of invasiveness of tumors depicted in panel B, which was determined by measuring the mean distance (micrometers) of invading tumor clusters away from the main tumor mass. (D) Top immunofluorescence of GFP and RFP (blue, DAPI) in U87HK2-GFP, U87 mixed, and U87HK2shRNA-RFP tumors. Bar, 28 µm. Bottom immunofluorescence of staining of the vasculature (vWF) of tumors. Bar, 39 µm. (E) Histopathology of U87HK2-GFP, U87 control, and U87HK2shRNA intracranial tumors with antibodies specific to vWF (blood vessels labeled with arrow; bar, 100 µm), VEGF (bar, 100 µm), <t>HIF1α</t> (positive cells labeled with arrow; bar, 50 µm), MIB1 (bar, 100 µm), cleaved caspase 3 (bar, 100 µm). The percentage of proliferating cells (MIB1) and apoptotic cells (cleaved caspase 3) are indicated above the photomicrographs.
    Hif1α, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore protease
    HK2 regulates GBM growth and invasion in an orthotopic xenograft model. (A) Survival curve of mice injected intracranially with 2 × 10 5 of U87 cells expressing HK2shRNA-RFP ( n = 10), U87 control cells ( n = 10), U87 cells overexpressing HK2-GFP ( n = 10), or a mix of U87 cells expressing HK2-GFP and HK2shRNA-RFP ( n = 6). (B) Hematoxylin and eosin stain of tumors described in A. Bars, 2,000 µm. Arrow is pointing to the small tumor in HK2shRNA-RFP tumors. (C) Hematoxylin and eosin micrographs depicting invasiveness of HK2shRNA tumors. The arrow is pointing at the invading tumor cells within the cerebellum. Bars: (top) 250 µm; (bottom) 100 µm. Bottom graph depicts the extent of invasiveness of tumors depicted in panel B, which was determined by measuring the mean distance (micrometers) of invading tumor clusters away from the main tumor mass. (D) Top immunofluorescence of GFP and RFP (blue, DAPI) in U87HK2-GFP, U87 mixed, and U87HK2shRNA-RFP tumors. Bar, 28 µm. Bottom immunofluorescence of staining of the vasculature (vWF) of tumors. Bar, 39 µm. (E) Histopathology of U87HK2-GFP, U87 control, and U87HK2shRNA intracranial tumors with antibodies specific to vWF (blood vessels labeled with arrow; bar, 100 µm), VEGF (bar, 100 µm), <t>HIF1α</t> (positive cells labeled with arrow; bar, 50 µm), MIB1 (bar, 100 µm), cleaved caspase 3 (bar, 100 µm). The percentage of proliferating cells (MIB1) and apoptotic cells (cleaved caspase 3) are indicated above the photomicrographs.
    Protease, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Cell Signaling Technology Inc complex iv
    HK2 regulates GBM growth and invasion in an orthotopic xenograft model. (A) Survival curve of mice injected intracranially with 2 × 10 5 of U87 cells expressing HK2shRNA-RFP ( n = 10), U87 control cells ( n = 10), U87 cells overexpressing HK2-GFP ( n = 10), or a mix of U87 cells expressing HK2-GFP and HK2shRNA-RFP ( n = 6). (B) Hematoxylin and eosin stain of tumors described in A. Bars, 2,000 µm. Arrow is pointing to the small tumor in HK2shRNA-RFP tumors. (C) Hematoxylin and eosin micrographs depicting invasiveness of HK2shRNA tumors. The arrow is pointing at the invading tumor cells within the cerebellum. Bars: (top) 250 µm; (bottom) 100 µm. Bottom graph depicts the extent of invasiveness of tumors depicted in panel B, which was determined by measuring the mean distance (micrometers) of invading tumor clusters away from the main tumor mass. (D) Top immunofluorescence of GFP and RFP (blue, DAPI) in U87HK2-GFP, U87 mixed, and U87HK2shRNA-RFP tumors. Bar, 28 µm. Bottom immunofluorescence of staining of the vasculature (vWF) of tumors. Bar, 39 µm. (E) Histopathology of U87HK2-GFP, U87 control, and U87HK2shRNA intracranial tumors with antibodies specific to vWF (blood vessels labeled with arrow; bar, 100 µm), VEGF (bar, 100 µm), <t>HIF1α</t> (positive cells labeled with arrow; bar, 50 µm), MIB1 (bar, 100 µm), cleaved caspase 3 (bar, 100 µm). The percentage of proliferating cells (MIB1) and apoptotic cells (cleaved caspase 3) are indicated above the photomicrographs.
    Complex Iv, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Level of pEGFR, EGFR and CCND1 protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.

    Journal: Carcinogenesis

    Article Title: EGFR pathway polymorphisms and bladder cancer susceptibility and prognosis

    doi: 10.1093/carcin/bgp077

    Figure Lengend Snippet: Level of pEGFR, EGFR and CCND1 protein in bladder tumors by genotype. Intensity of bladder tumor cells staining positively for pEGFR, EGFR and CCND1 is graphed by genotype for the EGFR_05 SNP. Staining intensity was scored on a scale of 0–4 by a single pathologist.

    Article Snippet: There were no significant independent effects of the SNPs tested in VEGF, EGF or CCND1.

    Techniques: Staining

    HK2 regulates GBM growth and invasion in an orthotopic xenograft model. (A) Survival curve of mice injected intracranially with 2 × 10 5 of U87 cells expressing HK2shRNA-RFP ( n = 10), U87 control cells ( n = 10), U87 cells overexpressing HK2-GFP ( n = 10), or a mix of U87 cells expressing HK2-GFP and HK2shRNA-RFP ( n = 6). (B) Hematoxylin and eosin stain of tumors described in A. Bars, 2,000 µm. Arrow is pointing to the small tumor in HK2shRNA-RFP tumors. (C) Hematoxylin and eosin micrographs depicting invasiveness of HK2shRNA tumors. The arrow is pointing at the invading tumor cells within the cerebellum. Bars: (top) 250 µm; (bottom) 100 µm. Bottom graph depicts the extent of invasiveness of tumors depicted in panel B, which was determined by measuring the mean distance (micrometers) of invading tumor clusters away from the main tumor mass. (D) Top immunofluorescence of GFP and RFP (blue, DAPI) in U87HK2-GFP, U87 mixed, and U87HK2shRNA-RFP tumors. Bar, 28 µm. Bottom immunofluorescence of staining of the vasculature (vWF) of tumors. Bar, 39 µm. (E) Histopathology of U87HK2-GFP, U87 control, and U87HK2shRNA intracranial tumors with antibodies specific to vWF (blood vessels labeled with arrow; bar, 100 µm), VEGF (bar, 100 µm), HIF1α (positive cells labeled with arrow; bar, 50 µm), MIB1 (bar, 100 µm), cleaved caspase 3 (bar, 100 µm). The percentage of proliferating cells (MIB1) and apoptotic cells (cleaved caspase 3) are indicated above the photomicrographs.

    Journal: The Journal of Experimental Medicine

    Article Title: Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in human glioblastoma multiforme

    doi: 10.1084/jem.20101470

    Figure Lengend Snippet: HK2 regulates GBM growth and invasion in an orthotopic xenograft model. (A) Survival curve of mice injected intracranially with 2 × 10 5 of U87 cells expressing HK2shRNA-RFP ( n = 10), U87 control cells ( n = 10), U87 cells overexpressing HK2-GFP ( n = 10), or a mix of U87 cells expressing HK2-GFP and HK2shRNA-RFP ( n = 6). (B) Hematoxylin and eosin stain of tumors described in A. Bars, 2,000 µm. Arrow is pointing to the small tumor in HK2shRNA-RFP tumors. (C) Hematoxylin and eosin micrographs depicting invasiveness of HK2shRNA tumors. The arrow is pointing at the invading tumor cells within the cerebellum. Bars: (top) 250 µm; (bottom) 100 µm. Bottom graph depicts the extent of invasiveness of tumors depicted in panel B, which was determined by measuring the mean distance (micrometers) of invading tumor clusters away from the main tumor mass. (D) Top immunofluorescence of GFP and RFP (blue, DAPI) in U87HK2-GFP, U87 mixed, and U87HK2shRNA-RFP tumors. Bar, 28 µm. Bottom immunofluorescence of staining of the vasculature (vWF) of tumors. Bar, 39 µm. (E) Histopathology of U87HK2-GFP, U87 control, and U87HK2shRNA intracranial tumors with antibodies specific to vWF (blood vessels labeled with arrow; bar, 100 µm), VEGF (bar, 100 µm), HIF1α (positive cells labeled with arrow; bar, 50 µm), MIB1 (bar, 100 µm), cleaved caspase 3 (bar, 100 µm). The percentage of proliferating cells (MIB1) and apoptotic cells (cleaved caspase 3) are indicated above the photomicrographs.

    Article Snippet: Membranes were probed overnight with the following antibodies: β-actin (1:20,000; Sigma-Aldrich); PDH-E1a (1:200; Abcam); VEGF (1:200; Santa Cruz Biotechnology, Inc.,); HIF1α (1:250; BD); and PKM2, HK2, HK1, and PHD2 (1:1,000; Cell Signaling Technology).

    Techniques: Mouse Assay, Injection, Expressing, H&E Stain, Immunofluorescence, Staining, Histopathology, Labeling