Journal: Nucleic Acids Research
Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
Figure Lengend Snippet: Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without NTP or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
Article Snippet: For phosphoryl donor preference determination, the phosphotransferase assays were carried out for 6 min with 6 nM PSTK and either no NTP or 20 mM ultrapure ( > 99%) NTP (ATP, ITP, GTP, CTP, UTP) (Sigma) or dATP (New England Biolabs).
Techniques: Activity Assay, Conditioned Place Preference, Standard Deviation