itp New England Biolabs Search Results


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  • 99
    New England Biolabs datp
    Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or <t>dATP</t> or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Datp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dnase i
    Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or <t>dATP</t> or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 56198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore ntp
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Ntp, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    New England Biolabs il 1b 511
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Il 1b 511, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    New England Biolabs bp dsdna ladder
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Bp Dsdna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs phusion dna polymerase
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Phusion Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs t4 dna ligase
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 38631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    New England Biolabs deoxynucleotide solution mix
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Deoxynucleotide Solution Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs protoscript ii reverse transcriptase
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Protoscript Ii Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs rnase h
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2919 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs t7 rna polymerase
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    T7 Rna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3044 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs hiscribe t7 high yield rna synthesis kit
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Hiscribe T7 High Yield Rna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    New England Biolabs m7g 5 ppp 5 g rna cap structure analog
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    M7g 5 Ppp 5 G Rna Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs neb 10 β competent e coli bacteria
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Neb 10 β Competent E Coli Bacteria, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer p atp
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore o nh2 deoxyinosine
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    O Nh2 Deoxyinosine, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dtt
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 29078 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor buffer components
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Buffer Components, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor mgcl2
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Mgcl2, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor vwr rlmb 005
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Vwr Rlmb 005, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    kcl  (Avantor)
    92
    Avantor kcl
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Kcl, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore apramycin apr
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Apramycin Apr, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore utp
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Utp, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore hygromycin hyg
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Hygromycin Hyg, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tobramycin tob
    Specificity of M. jannaschii <t>PSTK</t> for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without <t>NTP</t> or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.
    Tobramycin Tob, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without NTP or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.

    Journal: Nucleic Acids Research

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation

    doi: 10.1093/nar/gkm1134

    Figure Lengend Snippet: Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without NTP or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.

    Article Snippet: For phosphoryl donor preference determination, the phosphotransferase assays were carried out for 6 min with 6 nM PSTK and either no NTP or 20 mM ultrapure ( > 99%) NTP (ATP, ITP, GTP, CTP, UTP) (Sigma) or dATP (New England Biolabs).

    Techniques: Activity Assay, Conditioned Place Preference, Standard Deviation

    Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without NTP or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.

    Journal: Nucleic Acids Research

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation

    doi: 10.1093/nar/gkm1134

    Figure Lengend Snippet: Specificity of M. jannaschii PSTK for the phosphoryl donor. Initial velocities for the phosphotransferase activity of PSTK (6 nM PSTK without NTP or the addition of ATP, ITP, GTP, CTP, UTP or dATP or 200 nM PSTK with AMP-CPP or AMP-PCP) in the presence of the indicated phosphoryl donors (20 mM). Reactions proceeded for 6 min at 37°C and 1.5 min time points were taken and analyzed as in Figure 1 A and 2 . *ND, no detectable phosphorylation. Error bars represent the standard deviation of three experiments.

    Article Snippet: For phosphoryl donor preference determination, the phosphotransferase assays were carried out for 6 min with 6 nM PSTK and either no NTP or 20 mM ultrapure ( > 99%) NTP (ATP, ITP, GTP, CTP, UTP) (Sigma) or dATP (New England Biolabs).

    Techniques: Activity Assay, Conditioned Place Preference, Standard Deviation