isothermal reaction buffer Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs isothermal assembly reaction buffer
    Isothermal Assembly Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal assembly reaction buffer/product/New England Biolabs
    Average 90 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    isothermal assembly reaction buffer - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher isothermal reaction buffer
    Isothermal Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal reaction buffer/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    isothermal reaction buffer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    85
    Bio-Rad isothermal assembly reaction buffer
    Isothermal Assembly Reaction Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal assembly reaction buffer/product/Bio-Rad
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    isothermal assembly reaction buffer - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    96
    New England Biolabs isothermal amplification buffer ii pack
    Isothermal Amplification Buffer Ii Pack, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal amplification buffer ii pack/product/New England Biolabs
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer ii pack - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    96
    Millipore betaine
    Betaine, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 4005 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/betaine/product/Millipore
    Average 96 stars, based on 4005 article reviews
    Price from $9.99 to $1999.99
    betaine - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    94
    Qiagen cxxc5 transcripts
    Effects of siRNAs specific to <t>CXXC5</t> on cellular growth. MCF7 cells grown in 10% CD-FBS containing medium for 48 h were transiently transfected without (UT) or with 10 nM CtS or siRNA#10 in the absence (0.01% ethanol) or the presence of 10 −8 M E2 up to 72 h. ( a ) Cells were subjected to cell counting using a hemocytometer. Results, as the mean ± S.E. of three independent determinations, depict fold change in cellular growth compared with those observed with cells at time 0 in the absence of E2, which is set to 1. In ( a–f ) the asterisk with a superscript “a” indicates significant difference from the corresponding ethanol-treated group (-E2); whereas superscript “b” depicts a significant difference from CtS transfected cells in the presence of E2. ( b ) The cDNA library generated from total RNA (50 ng) isolated from transfected cells for 48 h was subjected to qPCR using a primer set specific to CXXC5. ( c ) Nuclear extracts from untransfected (UT) or transfected cells were subjected to WB using an antibody for CXXC5, ERα or HDAC1. NS denotes non-specific protein; Molecular mass in KDa is indicated. Transfected cells were subjected to cell counting ( d ) and MTT assay ( e ). In ( b , d , e) , results depicting the mean ± S.E. of three independent determinations indicate fold change in mRNA levels ( b ) or cell numbers ( d , e ) compared with CtS in the absence of E2, which is set to 1. Transfected MCF7 cells were subjected to flow cytometry ( f ) with results depicting the percent of cells in the G1, G2 and S phases, or to Annexin V assay followed by flow cytometry ( g ). In ( f , g ), results are the mean ± SEM of three independent experiments. ( h i ) Untransfected (UT) or transfected cells were also subjected to the cell death inducer camptothecin (2 nM) for 24 h without (−E2) or with E2 (E2). Cells were then subjected to Annexin V assay ( h ) with results as the mean ± S.E. of three independent experiments depicting percent change in apoptotic (upper right quadrant) and death (upper left quadrant) cells, or to WB ( i ) using a PARP1-specific antibody. P and CP denote the uncut and cut PARP1, respectively.
    Cxxc5 Transcripts, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cxxc5 transcripts/product/Qiagen
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cxxc5 transcripts - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of siRNAs specific to CXXC5 on cellular growth. MCF7 cells grown in 10% CD-FBS containing medium for 48 h were transiently transfected without (UT) or with 10 nM CtS or siRNA#10 in the absence (0.01% ethanol) or the presence of 10 −8 M E2 up to 72 h. ( a ) Cells were subjected to cell counting using a hemocytometer. Results, as the mean ± S.E. of three independent determinations, depict fold change in cellular growth compared with those observed with cells at time 0 in the absence of E2, which is set to 1. In ( a–f ) the asterisk with a superscript “a” indicates significant difference from the corresponding ethanol-treated group (-E2); whereas superscript “b” depicts a significant difference from CtS transfected cells in the presence of E2. ( b ) The cDNA library generated from total RNA (50 ng) isolated from transfected cells for 48 h was subjected to qPCR using a primer set specific to CXXC5. ( c ) Nuclear extracts from untransfected (UT) or transfected cells were subjected to WB using an antibody for CXXC5, ERα or HDAC1. NS denotes non-specific protein; Molecular mass in KDa is indicated. Transfected cells were subjected to cell counting ( d ) and MTT assay ( e ). In ( b , d , e) , results depicting the mean ± S.E. of three independent determinations indicate fold change in mRNA levels ( b ) or cell numbers ( d , e ) compared with CtS in the absence of E2, which is set to 1. Transfected MCF7 cells were subjected to flow cytometry ( f ) with results depicting the percent of cells in the G1, G2 and S phases, or to Annexin V assay followed by flow cytometry ( g ). In ( f , g ), results are the mean ± SEM of three independent experiments. ( h i ) Untransfected (UT) or transfected cells were also subjected to the cell death inducer camptothecin (2 nM) for 24 h without (−E2) or with E2 (E2). Cells were then subjected to Annexin V assay ( h ) with results as the mean ± S.E. of three independent experiments depicting percent change in apoptotic (upper right quadrant) and death (upper left quadrant) cells, or to WB ( i ) using a PARP1-specific antibody. P and CP denote the uncut and cut PARP1, respectively.

    Journal: Scientific Reports

    Article Title: CXXC5 as an unmethylated CpG dinucleotide binding protein contributes to estrogen-mediated cellular proliferation

    doi: 10.1038/s41598-020-62912-0

    Figure Lengend Snippet: Effects of siRNAs specific to CXXC5 on cellular growth. MCF7 cells grown in 10% CD-FBS containing medium for 48 h were transiently transfected without (UT) or with 10 nM CtS or siRNA#10 in the absence (0.01% ethanol) or the presence of 10 −8 M E2 up to 72 h. ( a ) Cells were subjected to cell counting using a hemocytometer. Results, as the mean ± S.E. of three independent determinations, depict fold change in cellular growth compared with those observed with cells at time 0 in the absence of E2, which is set to 1. In ( a–f ) the asterisk with a superscript “a” indicates significant difference from the corresponding ethanol-treated group (-E2); whereas superscript “b” depicts a significant difference from CtS transfected cells in the presence of E2. ( b ) The cDNA library generated from total RNA (50 ng) isolated from transfected cells for 48 h was subjected to qPCR using a primer set specific to CXXC5. ( c ) Nuclear extracts from untransfected (UT) or transfected cells were subjected to WB using an antibody for CXXC5, ERα or HDAC1. NS denotes non-specific protein; Molecular mass in KDa is indicated. Transfected cells were subjected to cell counting ( d ) and MTT assay ( e ). In ( b , d , e) , results depicting the mean ± S.E. of three independent determinations indicate fold change in mRNA levels ( b ) or cell numbers ( d , e ) compared with CtS in the absence of E2, which is set to 1. Transfected MCF7 cells were subjected to flow cytometry ( f ) with results depicting the percent of cells in the G1, G2 and S phases, or to Annexin V assay followed by flow cytometry ( g ). In ( f , g ), results are the mean ± SEM of three independent experiments. ( h i ) Untransfected (UT) or transfected cells were also subjected to the cell death inducer camptothecin (2 nM) for 24 h without (−E2) or with E2 (E2). Cells were then subjected to Annexin V assay ( h ) with results as the mean ± S.E. of three independent experiments depicting percent change in apoptotic (upper right quadrant) and death (upper left quadrant) cells, or to WB ( i ) using a PARP1-specific antibody. P and CP denote the uncut and cut PARP1, respectively.

    Article Snippet: In our previous study , we had evaluated four different siRNAs that target CXXC5 transcripts (FlexitubeGene Solution, Qiagen siRNA #2, 7, 9 and 10) as well as a scrambled siRNA control (CtS) to alter levels of CXXC5 transcript and protein.

    Techniques: Transfection, Cell Counting, cDNA Library Assay, Generated, Isolation, Real-time Polymerase Chain Reaction, Western Blot, MTT Assay, Flow Cytometry, Annexin V Assay

    Analysis of Gene Expression with the nCounter PanCancer Pathway Panel. MCF7 cells grown in 12-well tissue culture plates (9 × 10 4 cells/well) in 10% CD-FBS medium for 48 h were transiently transfected using 10 nM of CtS or siRNA#10, generating four treatment groups: Group (1) CtS and −E2; (2) #10 and −E2; (3) CtS and +E2; (4) #10 and +E2. Forty-eight hours after transfection, cells were treated without (ethanol, 0.01%) as vehicle control or with 10 −8 M of E2 for 3 hours. Total RNA (50 ng) was subjected to the nCounter PanCancer Pathway Panel gene expression analysis with nCounter Digital Analyzer. Quality control, data normalization and differential expression analyses were carried out using Nanostring nSolver 3.0 Analysis software together with its Advanced Analysis plug-in. Each treatment is depicted as heat maps with increasing (red) and decreasing levels (blue) and are the mean of three independent experiments in the log2 scale. (a) To assess differentially expressed genes mediated by CXXC5 in the absence of E2 (#10 −E2), nanostring results obtained from cells transfected with siRNA#10 in the absence of E2 (Group 2) was normalized to those from cells transfected with CtS in the absence of E2 (Group 1). (b) The effects of E2 on gene expression (CtS and +E2) were assessed by normalizing nanostring results obtained from cells transfected with CtS in the presence of E2 (Group 3) to those from cells transfected with CtS in the absence of E2 (Group 1). (c) To reveal the impact of CXXC5 on gene expressions in response to E2, we compared differentially expressed genes observed in the presence (CtS and +E2) and the absence (#10 and +E2) of CXXC5, the latter which was obtained by normalizing nanostring results obtained from cells transfected with siRNA#10 in the presence of E2 (Group 4) to those from cells transfected with siRNA#10 in the absence of E2 (Group 2).

    Journal: Scientific Reports

    Article Title: CXXC5 as an unmethylated CpG dinucleotide binding protein contributes to estrogen-mediated cellular proliferation

    doi: 10.1038/s41598-020-62912-0

    Figure Lengend Snippet: Analysis of Gene Expression with the nCounter PanCancer Pathway Panel. MCF7 cells grown in 12-well tissue culture plates (9 × 10 4 cells/well) in 10% CD-FBS medium for 48 h were transiently transfected using 10 nM of CtS or siRNA#10, generating four treatment groups: Group (1) CtS and −E2; (2) #10 and −E2; (3) CtS and +E2; (4) #10 and +E2. Forty-eight hours after transfection, cells were treated without (ethanol, 0.01%) as vehicle control or with 10 −8 M of E2 for 3 hours. Total RNA (50 ng) was subjected to the nCounter PanCancer Pathway Panel gene expression analysis with nCounter Digital Analyzer. Quality control, data normalization and differential expression analyses were carried out using Nanostring nSolver 3.0 Analysis software together with its Advanced Analysis plug-in. Each treatment is depicted as heat maps with increasing (red) and decreasing levels (blue) and are the mean of three independent experiments in the log2 scale. (a) To assess differentially expressed genes mediated by CXXC5 in the absence of E2 (#10 −E2), nanostring results obtained from cells transfected with siRNA#10 in the absence of E2 (Group 2) was normalized to those from cells transfected with CtS in the absence of E2 (Group 1). (b) The effects of E2 on gene expression (CtS and +E2) were assessed by normalizing nanostring results obtained from cells transfected with CtS in the presence of E2 (Group 3) to those from cells transfected with CtS in the absence of E2 (Group 1). (c) To reveal the impact of CXXC5 on gene expressions in response to E2, we compared differentially expressed genes observed in the presence (CtS and +E2) and the absence (#10 and +E2) of CXXC5, the latter which was obtained by normalizing nanostring results obtained from cells transfected with siRNA#10 in the presence of E2 (Group 4) to those from cells transfected with siRNA#10 in the absence of E2 (Group 2).

    Article Snippet: In our previous study , we had evaluated four different siRNAs that target CXXC5 transcripts (FlexitubeGene Solution, Qiagen siRNA #2, 7, 9 and 10) as well as a scrambled siRNA control (CtS) to alter levels of CXXC5 transcript and protein.

    Techniques: Expressing, Transfection, Software

    Purification and interaction with DNA of the recombinant full-length CXXC5 (FL-CXXC5) and the CXXC domain (CXXC-D) proteins. CXXC-D (a) and FL-CXXC5 (c) , expressed in bacteria and purified sequentially with ion exchange, heparin, and size-exclusion chromatographies, were loaded onto an SDS-PAGE gel (4–20% gradient) and stained with InstantBlue coomassie dye. “MW” indicates molecular masses in kDa. 10 µM CXXC-D ( b ) or FL-CXXC5 ( d ) was subjected to isothermal titration calorimetry (ITC) using a 300 µM double-stranded DNA fragment that bears a central unmethylated CG dinucleotide (5′-GTGATAC CG GATCAGT-3′). (e,f) The binding of FL-CXXC5 was also assessed with ITC using a double-stranded DNA fragment with the central mCG or AT nucleotides embedded into the same surrounding DNA sequence. To ensure that sequences surrounding the central nucleotides have no effect on the ability of FL-CXXC5 to bind to DNA, a double-stranded DNA fragment with a distinct surrounding sequence (5′-GAGAGAC xx GTCTCTC-3′) bearing the central CG ( G ), mCG ( h ) or AT ( i ) nucleotides was subjected to ITC. Results are the mean ± S.E. of two experiments. NB denotes no detectable binding.

    Journal: Scientific Reports

    Article Title: CXXC5 as an unmethylated CpG dinucleotide binding protein contributes to estrogen-mediated cellular proliferation

    doi: 10.1038/s41598-020-62912-0

    Figure Lengend Snippet: Purification and interaction with DNA of the recombinant full-length CXXC5 (FL-CXXC5) and the CXXC domain (CXXC-D) proteins. CXXC-D (a) and FL-CXXC5 (c) , expressed in bacteria and purified sequentially with ion exchange, heparin, and size-exclusion chromatographies, were loaded onto an SDS-PAGE gel (4–20% gradient) and stained with InstantBlue coomassie dye. “MW” indicates molecular masses in kDa. 10 µM CXXC-D ( b ) or FL-CXXC5 ( d ) was subjected to isothermal titration calorimetry (ITC) using a 300 µM double-stranded DNA fragment that bears a central unmethylated CG dinucleotide (5′-GTGATAC CG GATCAGT-3′). (e,f) The binding of FL-CXXC5 was also assessed with ITC using a double-stranded DNA fragment with the central mCG or AT nucleotides embedded into the same surrounding DNA sequence. To ensure that sequences surrounding the central nucleotides have no effect on the ability of FL-CXXC5 to bind to DNA, a double-stranded DNA fragment with a distinct surrounding sequence (5′-GAGAGAC xx GTCTCTC-3′) bearing the central CG ( G ), mCG ( h ) or AT ( i ) nucleotides was subjected to ITC. Results are the mean ± S.E. of two experiments. NB denotes no detectable binding.

    Article Snippet: In our previous study , we had evaluated four different siRNAs that target CXXC5 transcripts (FlexitubeGene Solution, Qiagen siRNA #2, 7, 9 and 10) as well as a scrambled siRNA control (CtS) to alter levels of CXXC5 transcript and protein.

    Techniques: Purification, Recombinant, SDS Page, Staining, Isothermal Titration Calorimetry, Binding Assay, Sequencing

    Clinicopathological relevance of CXXC5 expressions in breast cancer patients. ( a ) CXXC5 expression in breast cancer (BC) as compared to normal breast tissue from METABRIC. (b) CXXC5 expression in ER-negative versus ER-positive breast cancer patients from METABRIC. (c) CXXC5 expression in different molecular subtypes of tumors of breast cancer patients from METABRIC. (d) Pearson correlation analysis between mRNA expressions of CXXC5 and ESR1 (ERα) in patients from METABRIC. (e) Pearson correlation analysis between mRNA expression of CXXC5 and ERα protein level in breast cancer patients from TCGA. (f) Kaplan Meier survival analysis of basal breast cancer patients based on CXXC5 expression separated from the median.

    Journal: Scientific Reports

    Article Title: CXXC5 as an unmethylated CpG dinucleotide binding protein contributes to estrogen-mediated cellular proliferation

    doi: 10.1038/s41598-020-62912-0

    Figure Lengend Snippet: Clinicopathological relevance of CXXC5 expressions in breast cancer patients. ( a ) CXXC5 expression in breast cancer (BC) as compared to normal breast tissue from METABRIC. (b) CXXC5 expression in ER-negative versus ER-positive breast cancer patients from METABRIC. (c) CXXC5 expression in different molecular subtypes of tumors of breast cancer patients from METABRIC. (d) Pearson correlation analysis between mRNA expressions of CXXC5 and ESR1 (ERα) in patients from METABRIC. (e) Pearson correlation analysis between mRNA expression of CXXC5 and ERα protein level in breast cancer patients from TCGA. (f) Kaplan Meier survival analysis of basal breast cancer patients based on CXXC5 expression separated from the median.

    Article Snippet: In our previous study , we had evaluated four different siRNAs that target CXXC5 transcripts (FlexitubeGene Solution, Qiagen siRNA #2, 7, 9 and 10) as well as a scrambled siRNA control (CtS) to alter levels of CXXC5 transcript and protein.

    Techniques: Expressing

    Electrophoretic mobility shift (EMSA) and reporter assay. ( a ) To assess the ability of the recombinant FL-CXXC5 or its CXXC domain (CXXC-D), 50 μM DNA with the central unmethylated ( CG ) or methylated ( mCG ) CpG dinucleotides was mixed with FL-CXXC5 at 1:0.5, 1:1, 1:2, 1:4 and 1:8 molar ratios or with CCCX-D at 1:4 molar ratios. Samples were run onto 5% native TBE gel, stained and visualized with UV spectrometry. “M” represents the molecular marker. “Free” denotes unbound DNA; “Shifted” indicates DNA bound protein. A representative experiment performed with two independent times is shown. (b) To assess the intrinsic transcription regulatory function of FL-CXXC5, pGal4-RE Luciferase Reporter vector (pGal4RE-Luc, 125 ng) containing tandem Gal4 response elements (Gal4-RE) juxtaposed to a simple TATA box promoter that drives the expression of the firefly Luciferase enzyme cDNA together with an expression vector (75 ng) bearing Gal4 DBD , VP16, FL-CXXC5, WT-MeCP2 or ERα-EF domain cDNA or Gal4 DBD -VP16, Gal4 DBD -CXXC5, Gal4 DBD -MeCP2 or Gal4 DBD -ERαEF cDNA transfected into MCF7 cells. MCF7 cells grown in 10% CD-FBS for 48 h were transfected with expression vector bearing ERαEF cDNA or Gal4 DBD -ERαEF and were treated without (%0.01 ethanol) or with 10 −8 M E2 for 24 h. Transfection efficiency was monitored with the co-expression of a reporter plasmid bearing the Renilla Luciferase enzyme cDNA (0.5 ng). Results indicating relative firefly/ Renilla luciferase activity determined using a dual luciferase assay kit are the mean ± S.E. of three independent experiments performed in duplicate. The asterisk denotes significant differences compared to responses observed with Gal4-RE, which is set to 1. (c) Cells were also transfected with pGal4RE-Luc together with the expression vector bearing none (empty vector, EV), Gal4 DBD -VP16 and/or Gal4 DBD -CXXC5 cDNA. In transfections, we used a total of 300 ng expression vector (1 denotes 75 ng), for which appropriate amounts of the parent expression vector (EV) were supplemented to equalize the total plasmid DNA amount. Results presented as relative firefly/ Renilla luciferase levels indicate percent change and are the mean ± S.E. of three independent experiments performed in duplicate. The asterisk denotes significant differences from responses observed with Gal4 DBD -VP16, which was set to 100%.

    Journal: Scientific Reports

    Article Title: CXXC5 as an unmethylated CpG dinucleotide binding protein contributes to estrogen-mediated cellular proliferation

    doi: 10.1038/s41598-020-62912-0

    Figure Lengend Snippet: Electrophoretic mobility shift (EMSA) and reporter assay. ( a ) To assess the ability of the recombinant FL-CXXC5 or its CXXC domain (CXXC-D), 50 μM DNA with the central unmethylated ( CG ) or methylated ( mCG ) CpG dinucleotides was mixed with FL-CXXC5 at 1:0.5, 1:1, 1:2, 1:4 and 1:8 molar ratios or with CCCX-D at 1:4 molar ratios. Samples were run onto 5% native TBE gel, stained and visualized with UV spectrometry. “M” represents the molecular marker. “Free” denotes unbound DNA; “Shifted” indicates DNA bound protein. A representative experiment performed with two independent times is shown. (b) To assess the intrinsic transcription regulatory function of FL-CXXC5, pGal4-RE Luciferase Reporter vector (pGal4RE-Luc, 125 ng) containing tandem Gal4 response elements (Gal4-RE) juxtaposed to a simple TATA box promoter that drives the expression of the firefly Luciferase enzyme cDNA together with an expression vector (75 ng) bearing Gal4 DBD , VP16, FL-CXXC5, WT-MeCP2 or ERα-EF domain cDNA or Gal4 DBD -VP16, Gal4 DBD -CXXC5, Gal4 DBD -MeCP2 or Gal4 DBD -ERαEF cDNA transfected into MCF7 cells. MCF7 cells grown in 10% CD-FBS for 48 h were transfected with expression vector bearing ERαEF cDNA or Gal4 DBD -ERαEF and were treated without (%0.01 ethanol) or with 10 −8 M E2 for 24 h. Transfection efficiency was monitored with the co-expression of a reporter plasmid bearing the Renilla Luciferase enzyme cDNA (0.5 ng). Results indicating relative firefly/ Renilla luciferase activity determined using a dual luciferase assay kit are the mean ± S.E. of three independent experiments performed in duplicate. The asterisk denotes significant differences compared to responses observed with Gal4-RE, which is set to 1. (c) Cells were also transfected with pGal4RE-Luc together with the expression vector bearing none (empty vector, EV), Gal4 DBD -VP16 and/or Gal4 DBD -CXXC5 cDNA. In transfections, we used a total of 300 ng expression vector (1 denotes 75 ng), for which appropriate amounts of the parent expression vector (EV) were supplemented to equalize the total plasmid DNA amount. Results presented as relative firefly/ Renilla luciferase levels indicate percent change and are the mean ± S.E. of three independent experiments performed in duplicate. The asterisk denotes significant differences from responses observed with Gal4 DBD -VP16, which was set to 100%.

    Article Snippet: In our previous study , we had evaluated four different siRNAs that target CXXC5 transcripts (FlexitubeGene Solution, Qiagen siRNA #2, 7, 9 and 10) as well as a scrambled siRNA control (CtS) to alter levels of CXXC5 transcript and protein.

    Techniques: Electrophoretic Mobility Shift Assay, Reporter Assay, Recombinant, Methylation, Staining, Marker, Luciferase, Plasmid Preparation, Expressing, Transfection, Activity Assay