isoflurane Search Results


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    Abbott Laboratories isoflurane
    <t>Isoflurane</t> increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P
    Isoflurane, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore isoflurane
    <t>Isoflurane</t> increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P
    Isoflurane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/Millipore
    Average 99 stars, based on 1 article reviews
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    86
    Baxter Healthcare isoflurane
    The effects of <t>isoflurane</t> on wild-type IKs and A341V mutant under heterozygous conditions in HL-1 cells. The cDNA ratios for each subunit used for transfection were the same for those used in . (A) Representative current traces of 2KCNQ1+KCNE1
    Isoflurane, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/Baxter Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoflurane - by Bioz Stars, 2021-03
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    86
    David Kopf Instruments isoflurane
    ( A ) Peak value of BOLD, DfMRI (b = 1,800 s/mm 2 ) responses and ( B ) ΣLFP, ( C ) time-to-peak of BOLD and DfMRI by 7-Hz stimulation under 1.0%, 1.5%, and 2.0% <t>isoflurane</t> and ( D ) FWHM of BOLD and DfMRI responses; * P
    Isoflurane, supplied by David Kopf Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/David Kopf Instruments
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    Image Search Results


    Isoflurane increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Immunohistochemistry, Mouse Assay, Staining

    Interleukin (IL)-11 is critical for isoflurane post-conditioning mediated reduction in renal neutrophil infiltration after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for immunohistochemistry (brown staining) for neutrophil infiltration (200×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min. renal ischemia and 24-h reperfusion. B. Quantifications of infiltrated neutrophils per 200× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane post-conditioning mediated reduction in renal neutrophil infiltration after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for immunohistochemistry (brown staining) for neutrophil infiltration (200×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min. renal ischemia and 24-h reperfusion. B. Quantifications of infiltrated neutrophils per 200× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Immunohistochemistry, Staining, Mouse Assay

    Interleukin (IL)-11 is critical for isoflurane-mediated reduction in renal tubular necrosis after ischemia and reperfusion (IR) A. Representative photomicrographs of five to six experiments for hematoxylin and eosin staining (magnification 200×) of kidneys of IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion (I/R). B. Summary of Jablonski scale renal injury scores (N = 4, graded from hematoxylin and eosin staining, scale 0-4) for mice subjected to renal I/R. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane-mediated reduction in renal tubular necrosis after ischemia and reperfusion (IR) A. Representative photomicrographs of five to six experiments for hematoxylin and eosin staining (magnification 200×) of kidneys of IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion (I/R). B. Summary of Jablonski scale renal injury scores (N = 4, graded from hematoxylin and eosin staining, scale 0-4) for mice subjected to renal I/R. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Staining, Mouse Assay

    Interleukin (IL)-11 is critical for isoflurane postconditioning mediated reduction in renal tubular apoptosis after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (representing apoptotic nuclei, magnification 100×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO [knockout]) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion. B. Quantifications of apoptotic cells per 100× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane postconditioning mediated reduction in renal tubular apoptosis after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (representing apoptotic nuclei, magnification 100×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO [knockout]) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion. B. Quantifications of apoptotic cells per 100× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: TUNEL Assay, Staining, Mouse Assay, Knock-Out

    Isoflurane induces interleukin (IL)-11 in human proximal tubule (HK-2) cells via transforming growth factor-beta 1 (TGF-β1) A. IL-11 messenger ribonucleic acid (mRNA) (detected with reverse transcription polymerase chain reaction (RTPCR)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas plus immunoglobulin G (IgG) isotype antibody treated controls. B. IL-11 protein (detected with enzyme-linked immunosorbent assay (ELISA)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane induces interleukin (IL)-11 in human proximal tubule (HK-2) cells via transforming growth factor-beta 1 (TGF-β1) A. IL-11 messenger ribonucleic acid (mRNA) (detected with reverse transcription polymerase chain reaction (RTPCR)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas plus immunoglobulin G (IgG) isotype antibody treated controls. B. IL-11 protein (detected with enzyme-linked immunosorbent assay (ELISA)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay

    Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) expression in primary culture of mouse proximal tubule cells via transforming growth factor-beta 1 (TGF-β1) IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in primary culture of mouse proximal tubule cells treated with 2.5% isoflurane for 6 h (N = 4). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas and immunoglobulin G (IgG) isotype antibody treated controls. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was also quantified to normalize lane loading. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) expression in primary culture of mouse proximal tubule cells via transforming growth factor-beta 1 (TGF-β1) IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in primary culture of mouse proximal tubule cells treated with 2.5% isoflurane for 6 h (N = 4). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas and immunoglobulin G (IgG) isotype antibody treated controls. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was also quantified to normalize lane loading. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Isoflurane increases interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in mouse kidney via transforming growth factor-beta 1 (TGF-β1) signaling Naïve IL-11 wild-type (WT) mice were exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h. A. Representative bands for IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in mouse kidney (N = 4). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as an internal loading control. B. Kidney lysate IL-11 protein (detected by enzyme-linked immunosorbent assay (ELISA)) in IL-11 naïve WT mice exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h (N = 4). To neutralize TGF-β1 in vivo , some IL-11 WT mice were injected with 5 mg/kg monoclonal anti-TGF-β1 (MAB240) antibody intravenous injection. TGF-β1 neutralization prevented the induction of IL-11 after isoflurane anesthesia. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane increases interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in mouse kidney via transforming growth factor-beta 1 (TGF-β1) signaling Naïve IL-11 wild-type (WT) mice were exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h. A. Representative bands for IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in mouse kidney (N = 4). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as an internal loading control. B. Kidney lysate IL-11 protein (detected by enzyme-linked immunosorbent assay (ELISA)) in IL-11 naïve WT mice exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h (N = 4). To neutralize TGF-β1 in vivo , some IL-11 WT mice were injected with 5 mg/kg monoclonal anti-TGF-β1 (MAB240) antibody intravenous injection. TGF-β1 neutralization prevented the induction of IL-11 after isoflurane anesthesia. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, In Vivo, Injection, Neutralization

    Interleukin (IL)-11 is critical for isoflurane-mediated renal protection against ischemic acute kidney injury Plasma creatinine levels from IL-11 wild-type (WT) or IL-11 deficient (KO) mice subjected to 30 min renal ischemia and 24 h reperfusion (N = 4-6 per group). After renal ischemia reperfusion (RIR), mice were further anesthetized with 1.2% isoflurane (ISO) or with equi-anesthetic dose of pentobarbital (PB). Some IL-11 receptor (IL-11R) WT mice were pretreated with an IL-11 neutralizing antibody (1 mg/kg, intravenous injection) 20 min before reperfusion or sham-operation. Isoflurane postconditioning significantly reduced plasma creatinine after RIR injury in IL-11R WT mice. However, IL-11R deficiency or IL-11 neutralizing antibody prevented the renal protective effects of isoflurane post-conditioning. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane-mediated renal protection against ischemic acute kidney injury Plasma creatinine levels from IL-11 wild-type (WT) or IL-11 deficient (KO) mice subjected to 30 min renal ischemia and 24 h reperfusion (N = 4-6 per group). After renal ischemia reperfusion (RIR), mice were further anesthetized with 1.2% isoflurane (ISO) or with equi-anesthetic dose of pentobarbital (PB). Some IL-11 receptor (IL-11R) WT mice were pretreated with an IL-11 neutralizing antibody (1 mg/kg, intravenous injection) 20 min before reperfusion or sham-operation. Isoflurane postconditioning significantly reduced plasma creatinine after RIR injury in IL-11R WT mice. However, IL-11R deficiency or IL-11 neutralizing antibody prevented the renal protective effects of isoflurane post-conditioning. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Mouse Assay, Injection

    Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in human proximal tubule (HK-2) cells A and B. IL-11 mRNA measured with reverse transcription polymerase chain reaction (RTPCR) in HK-2 cells treated with 0-2.5% isoflurane for 6 h (A. N = 6) or 2.5% isoflurane for 0-6 h (B, N = 6). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was quantified to normalize lane loading. Data are presented as means ± SD. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in human proximal tubule (HK-2) cells A and B. IL-11 mRNA measured with reverse transcription polymerase chain reaction (RTPCR) in HK-2 cells treated with 0-2.5% isoflurane for 6 h (A. N = 6) or 2.5% isoflurane for 0-6 h (B, N = 6). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was quantified to normalize lane loading. Data are presented as means ± SD. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    The effects of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions in HL-1 cells. The cDNA ratios for each subunit used for transfection were the same for those used in . (A) Representative current traces of 2KCNQ1+KCNE1

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The effects of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions in HL-1 cells. The cDNA ratios for each subunit used for transfection were the same for those used in . (A) Representative current traces of 2KCNQ1+KCNE1

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Mutagenesis, Transfection

    Summary of the inhibitory effects of isoflurane under heterozygous conditions and the impact of A341V on current density. (A) Summary of the inhibitory effects of isoflurane on 2KCNQ1+KCNE1 and A341V+KCNQ1+KCNE1 at +60 mV. Similar to the homozygous conditions

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Summary of the inhibitory effects of isoflurane under heterozygous conditions and the impact of A341V on current density. (A) Summary of the inhibitory effects of isoflurane on 2KCNQ1+KCNE1 and A341V+KCNQ1+KCNE1 at +60 mV. Similar to the homozygous conditions

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    Effects on isoflurane on whole-cell potassium currents (IKs) fusion proteins. A. Schematic of the fusion proteins. In the MK24 protein, the C-terminus of KCNE1 was linked to the N-terminus of KCNQ1, and in the MKK44, the C-terminus of KCNE1 was linked

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Effects on isoflurane on whole-cell potassium currents (IKs) fusion proteins. A. Schematic of the fusion proteins. In the MK24 protein, the C-terminus of KCNE1 was linked to the N-terminus of KCNQ1, and in the MKK44, the C-terminus of KCNE1 was linked

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    The impact of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions. Whole-cell currents were monitored in HEK293 cells expressing A341V+KCNQ1+KCNE1 (2.4 μg of complementary DNA [cDNA] for each subunit) and compared to those

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The impact of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions. Whole-cell currents were monitored in HEK293 cells expressing A341V+KCNQ1+KCNE1 (2.4 μg of complementary DNA [cDNA] for each subunit) and compared to those

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Mutagenesis, Expressing

    Role of F340 in determining anesthetic sensitivity. The inhibitory effects of isoflurane (0.5 mM) were determined in two KCNQ1 mutants, F340A and F340C, as shown in panels (A) and (B), respectively. The results are summarized in (C). Current inhibition

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Role of F340 in determining anesthetic sensitivity. The inhibitory effects of isoflurane (0.5 mM) were determined in two KCNQ1 mutants, F340A and F340C, as shown in panels (A) and (B), respectively. The results are summarized in (C). Current inhibition

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Inhibition

    Simulation of the cardiac action potential using the ten Tusscher model of human ventricular myocyte. (A) Simulated action potential under control condition (wild-type IKs) in the absence and presence (dashed lines) of isoflurane. (B) Simulated action potential

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Simulation of the cardiac action potential using the ten Tusscher model of human ventricular myocyte. (A) Simulated action potential under control condition (wild-type IKs) in the absence and presence (dashed lines) of isoflurane. (B) Simulated action potential

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HL-1 cardiomyocytes

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HL-1 cardiomyocytes

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    The impact of isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in a cardiac cell line, HL-1 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in control and in the presence of isoflurane is shown. The voltage protocol is as depicted in

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The impact of isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in a cardiac cell line, HL-1 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in control and in the presence of isoflurane is shown. The voltage protocol is as depicted in

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    The effect of isoflurane on KCNQ1 expressed alone in HL-1 cells. (A) Representative current traces of KCNQ1 are shown in the absence and presence of 0.5 mM isoflurane. (B) The effects of isoflurane on the corresponding voltage-dependence of activation

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The effect of isoflurane on KCNQ1 expressed alone in HL-1 cells. (A) Representative current traces of KCNQ1 are shown in the absence and presence of 0.5 mM isoflurane. (B) The effects of isoflurane on the corresponding voltage-dependence of activation

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Activation Assay

    The impact of isoflurane on wild-type IKs (KCNQ1+KCNE1) and A341V mutant in HEK293 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in the absence and presence of isoflurane (0.54 ± 0.05 mM; equivalent to 1.14 vol % at 22 °C)

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The impact of isoflurane on wild-type IKs (KCNQ1+KCNE1) and A341V mutant in HEK293 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in the absence and presence of isoflurane (0.54 ± 0.05 mM; equivalent to 1.14 vol % at 22 °C)

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Mutagenesis

    Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HEK293 Cells

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HEK293 Cells

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    ( A ) Peak value of BOLD, DfMRI (b = 1,800 s/mm 2 ) responses and ( B ) ΣLFP, ( C ) time-to-peak of BOLD and DfMRI by 7-Hz stimulation under 1.0%, 1.5%, and 2.0% isoflurane and ( D ) FWHM of BOLD and DfMRI responses; * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Water diffusion in brain cortex closely tracks underlying neuronal activity

    doi: 10.1073/pnas.1303178110

    Figure Lengend Snippet: ( A ) Peak value of BOLD, DfMRI (b = 1,800 s/mm 2 ) responses and ( B ) ΣLFP, ( C ) time-to-peak of BOLD and DfMRI by 7-Hz stimulation under 1.0%, 1.5%, and 2.0% isoflurane and ( D ) FWHM of BOLD and DfMRI responses; * P

    Article Snippet: In the electrophysiological study, the animals anesthetized with 2.5% isoflurane were placed in a stereotaxic frame (David Kopf Instruments), and a hole, centered at 3.7 ± 0.2 mm lateral and 0.7 mm rostral from the Bregma, was drilled on the left side of the skull.

    Techniques:

    ( A ) Peak value of BOLD, DfMRI (b = 1,800 s/mm 2 ) responses and ( B ) ΣLFP, ( C ) time-to-peak of BOLD and DfMRI, ( D ) FWHM of BOLD and DfMRI responses by four stimulation frequencies (3, 7, 10, and 13 Hz) under 1.5% isoflurane anesthesia; * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Water diffusion in brain cortex closely tracks underlying neuronal activity

    doi: 10.1073/pnas.1303178110

    Figure Lengend Snippet: ( A ) Peak value of BOLD, DfMRI (b = 1,800 s/mm 2 ) responses and ( B ) ΣLFP, ( C ) time-to-peak of BOLD and DfMRI, ( D ) FWHM of BOLD and DfMRI responses by four stimulation frequencies (3, 7, 10, and 13 Hz) under 1.5% isoflurane anesthesia; * P

    Article Snippet: In the electrophysiological study, the animals anesthetized with 2.5% isoflurane were placed in a stereotaxic frame (David Kopf Instruments), and a hole, centered at 3.7 ± 0.2 mm lateral and 0.7 mm rostral from the Bregma, was drilled on the left side of the skull.

    Techniques:

    ( A ) Five-block time courses for BOLD (SE and GE) and DfMRI responses, and LFPs (averaged over animals) recorded from a 7-Hz forepaw electrical stimulation paradigm under 1.5% isoflurane anesthesia. ( B ) Corresponding averaged responses (over animals and

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Water diffusion in brain cortex closely tracks underlying neuronal activity

    doi: 10.1073/pnas.1303178110

    Figure Lengend Snippet: ( A ) Five-block time courses for BOLD (SE and GE) and DfMRI responses, and LFPs (averaged over animals) recorded from a 7-Hz forepaw electrical stimulation paradigm under 1.5% isoflurane anesthesia. ( B ) Corresponding averaged responses (over animals and

    Article Snippet: In the electrophysiological study, the animals anesthetized with 2.5% isoflurane were placed in a stereotaxic frame (David Kopf Instruments), and a hole, centered at 3.7 ± 0.2 mm lateral and 0.7 mm rostral from the Bregma, was drilled on the left side of the skull.

    Techniques: Blocking Assay

    ( A ) Location of the electrophysiologically recorded area in the somatosensory cortex. ( B ) Group activation maps ( t contrast) for the BOLD and diffusion MRI signals obtained from a 7-Hz forepaw electrical stimulation paradigm under 1.5% isoflurane anesthesia

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Water diffusion in brain cortex closely tracks underlying neuronal activity

    doi: 10.1073/pnas.1303178110

    Figure Lengend Snippet: ( A ) Location of the electrophysiologically recorded area in the somatosensory cortex. ( B ) Group activation maps ( t contrast) for the BOLD and diffusion MRI signals obtained from a 7-Hz forepaw electrical stimulation paradigm under 1.5% isoflurane anesthesia

    Article Snippet: In the electrophysiological study, the animals anesthetized with 2.5% isoflurane were placed in a stereotaxic frame (David Kopf Instruments), and a hole, centered at 3.7 ± 0.2 mm lateral and 0.7 mm rostral from the Bregma, was drilled on the left side of the skull.

    Techniques: Activation Assay, Diffusion-based Assay, Magnetic Resonance Imaging

    ( A ) Time course of BOLD (GE and SE) and DfMRI (b = 250, 1,000, 1,800, and 2,600) response to 7-Hz stimulation with the nitroprusside administration under 1.5% isoflurane level. ( B ) Peak value of BOLD and DfMRI responses, ( C ) ∑LFP, and ( D ) ADC

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Water diffusion in brain cortex closely tracks underlying neuronal activity

    doi: 10.1073/pnas.1303178110

    Figure Lengend Snippet: ( A ) Time course of BOLD (GE and SE) and DfMRI (b = 250, 1,000, 1,800, and 2,600) response to 7-Hz stimulation with the nitroprusside administration under 1.5% isoflurane level. ( B ) Peak value of BOLD and DfMRI responses, ( C ) ∑LFP, and ( D ) ADC

    Article Snippet: In the electrophysiological study, the animals anesthetized with 2.5% isoflurane were placed in a stereotaxic frame (David Kopf Instruments), and a hole, centered at 3.7 ± 0.2 mm lateral and 0.7 mm rostral from the Bregma, was drilled on the left side of the skull.

    Techniques: