isoflurane Search Results


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    Abbott Laboratories isoflurane
    <t>Isoflurane</t> increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P
    Isoflurane, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/Abbott Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoflurane - by Bioz Stars, 2021-04
    86/100 stars
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    99
    Millipore isoflurane
    <t>Isoflurane</t> increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P
    Isoflurane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoflurane - by Bioz Stars, 2021-04
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    86
    Baxter Healthcare isoflurane
    The effects of <t>isoflurane</t> on wild-type IKs and A341V mutant under heterozygous conditions in HL-1 cells. The cDNA ratios for each subunit used for transfection were the same for those used in . (A) Representative current traces of 2KCNQ1+KCNE1
    Isoflurane, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/Baxter Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoflurane - by Bioz Stars, 2021-04
    86/100 stars
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    86
    Piramal isoflurane
    Physiological impact of anesthesia as a function of age. ( A ) Baseline blood glucose in mice at 7 (neonatal), 23 (2 days post-weaning), and 26 days of age. ( B ) Baseline blood ß-hydroxybutyrate in mice at 7 (neonatal), 23 (2 days post-weaning), and 26 days of age. ( C ) Respiratory rate of P23 mice as a function of time exposed to 1.5% <t>isoflurane.</t> ANOVA p-value
    Isoflurane, supplied by Piramal, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/Piramal
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    N/A
    Isoflurane is a halogenated ether with anesthetic properties While the mechanism of isoflurane anesthesia is not fully understood mice resistant to isoflurane anesthesia have an 86 decrease in gene expression
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    Image Search Results


    Isoflurane increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane increases interleukin (IL)-11 immunoreactivity in mouse kidney via transforming growth factor-beta 1 (TGF-β1) A. IL-11 immunohistochemistry (400× shown, N = 4) in kidneys from IL-11 receptor wild type (IL-11R WT) mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. IL-11 staining was darker in kidneys of mice anesthetized with isoflurane. TGF-β1 neutralizing antibody attenuated isoflurane-mediated increase in IL-11 immunoreactivity. Finally, IL-11 staining was not visible in kidneys from mice stained with negative isotype control antibodies (representative of four experiments). B. Quantifications of renal tubular IL-11 staining in mice anesthetized with pentobarbital or with 1.2% isoflurane for 4 h. Kidney IL-11 immunoreactivity significantly increased in mice anesthetized with isoflurane and attenuated with TGF-β1 neutralizing antibody. # P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Immunohistochemistry, Mouse Assay, Staining

    Interleukin (IL)-11 is critical for isoflurane post-conditioning mediated reduction in renal neutrophil infiltration after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for immunohistochemistry (brown staining) for neutrophil infiltration (200×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min. renal ischemia and 24-h reperfusion. B. Quantifications of infiltrated neutrophils per 200× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane post-conditioning mediated reduction in renal neutrophil infiltration after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for immunohistochemistry (brown staining) for neutrophil infiltration (200×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min. renal ischemia and 24-h reperfusion. B. Quantifications of infiltrated neutrophils per 200× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Immunohistochemistry, Staining, Mouse Assay

    Interleukin (IL)-11 is critical for isoflurane-mediated reduction in renal tubular necrosis after ischemia and reperfusion (IR) A. Representative photomicrographs of five to six experiments for hematoxylin and eosin staining (magnification 200×) of kidneys of IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion (I/R). B. Summary of Jablonski scale renal injury scores (N = 4, graded from hematoxylin and eosin staining, scale 0-4) for mice subjected to renal I/R. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane-mediated reduction in renal tubular necrosis after ischemia and reperfusion (IR) A. Representative photomicrographs of five to six experiments for hematoxylin and eosin staining (magnification 200×) of kidneys of IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion (I/R). B. Summary of Jablonski scale renal injury scores (N = 4, graded from hematoxylin and eosin staining, scale 0-4) for mice subjected to renal I/R. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Staining, Mouse Assay

    Interleukin (IL)-11 is critical for isoflurane postconditioning mediated reduction in renal tubular apoptosis after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (representing apoptotic nuclei, magnification 100×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO [knockout]) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion. B. Quantifications of apoptotic cells per 100× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane postconditioning mediated reduction in renal tubular apoptosis after ischemia and reperfusion A. Representative photomicrographs of four to six experiments for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (representing apoptotic nuclei, magnification 100×) from kidneys IL-11 receptor wild-type (IL-11R WT) mice, IL-11 receptor deficient (IL-11R KO [knockout]) mice and IL-11R WT mice pretreated with IL-11 neutralizing antibody and subjected to 30 min renal ischemia and 24-h reperfusion. B. Quantifications of apoptotic cells per 100× field in the kidneys of mice after renal ischemia reperfusion (RIR). * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: TUNEL Assay, Staining, Mouse Assay, Knock-Out

    Isoflurane induces interleukin (IL)-11 in human proximal tubule (HK-2) cells via transforming growth factor-beta 1 (TGF-β1) A. IL-11 messenger ribonucleic acid (mRNA) (detected with reverse transcription polymerase chain reaction (RTPCR)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas plus immunoglobulin G (IgG) isotype antibody treated controls. B. IL-11 protein (detected with enzyme-linked immunosorbent assay (ELISA)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane induces interleukin (IL)-11 in human proximal tubule (HK-2) cells via transforming growth factor-beta 1 (TGF-β1) A. IL-11 messenger ribonucleic acid (mRNA) (detected with reverse transcription polymerase chain reaction (RTPCR)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas plus immunoglobulin G (IgG) isotype antibody treated controls. B. IL-11 protein (detected with enzyme-linked immunosorbent assay (ELISA)) expression in HK-2 cells treated with 2.5% isoflurane for 6 h (N = 6). * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay

    Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) expression in primary culture of mouse proximal tubule cells via transforming growth factor-beta 1 (TGF-β1) IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in primary culture of mouse proximal tubule cells treated with 2.5% isoflurane for 6 h (N = 4). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas and immunoglobulin G (IgG) isotype antibody treated controls. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was also quantified to normalize lane loading. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) expression in primary culture of mouse proximal tubule cells via transforming growth factor-beta 1 (TGF-β1) IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in primary culture of mouse proximal tubule cells treated with 2.5% isoflurane for 6 h (N = 4). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in IL-11 expression over carrier gas and immunoglobulin G (IgG) isotype antibody treated controls. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was also quantified to normalize lane loading. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Isoflurane increases interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in mouse kidney via transforming growth factor-beta 1 (TGF-β1) signaling Naïve IL-11 wild-type (WT) mice were exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h. A. Representative bands for IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in mouse kidney (N = 4). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as an internal loading control. B. Kidney lysate IL-11 protein (detected by enzyme-linked immunosorbent assay (ELISA)) in IL-11 naïve WT mice exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h (N = 4). To neutralize TGF-β1 in vivo , some IL-11 WT mice were injected with 5 mg/kg monoclonal anti-TGF-β1 (MAB240) antibody intravenous injection. TGF-β1 neutralization prevented the induction of IL-11 after isoflurane anesthesia. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane increases interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in mouse kidney via transforming growth factor-beta 1 (TGF-β1) signaling Naïve IL-11 wild-type (WT) mice were exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h. A. Representative bands for IL-11 mRNA (reverse transcription polymerase chain reaction (RTPCR)) expression in mouse kidney (N = 4). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as an internal loading control. B. Kidney lysate IL-11 protein (detected by enzyme-linked immunosorbent assay (ELISA)) in IL-11 naïve WT mice exposed to pentobarbital (PB) or 1.2% isoflurane (ISO) for 4 h (N = 4). To neutralize TGF-β1 in vivo , some IL-11 WT mice were injected with 5 mg/kg monoclonal anti-TGF-β1 (MAB240) antibody intravenous injection. TGF-β1 neutralization prevented the induction of IL-11 after isoflurane anesthesia. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, In Vivo, Injection, Neutralization

    Interleukin (IL)-11 is critical for isoflurane-mediated renal protection against ischemic acute kidney injury Plasma creatinine levels from IL-11 wild-type (WT) or IL-11 deficient (KO) mice subjected to 30 min renal ischemia and 24 h reperfusion (N = 4-6 per group). After renal ischemia reperfusion (RIR), mice were further anesthetized with 1.2% isoflurane (ISO) or with equi-anesthetic dose of pentobarbital (PB). Some IL-11 receptor (IL-11R) WT mice were pretreated with an IL-11 neutralizing antibody (1 mg/kg, intravenous injection) 20 min before reperfusion or sham-operation. Isoflurane postconditioning significantly reduced plasma creatinine after RIR injury in IL-11R WT mice. However, IL-11R deficiency or IL-11 neutralizing antibody prevented the renal protective effects of isoflurane post-conditioning. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Interleukin (IL)-11 is critical for isoflurane-mediated renal protection against ischemic acute kidney injury Plasma creatinine levels from IL-11 wild-type (WT) or IL-11 deficient (KO) mice subjected to 30 min renal ischemia and 24 h reperfusion (N = 4-6 per group). After renal ischemia reperfusion (RIR), mice were further anesthetized with 1.2% isoflurane (ISO) or with equi-anesthetic dose of pentobarbital (PB). Some IL-11 receptor (IL-11R) WT mice were pretreated with an IL-11 neutralizing antibody (1 mg/kg, intravenous injection) 20 min before reperfusion or sham-operation. Isoflurane postconditioning significantly reduced plasma creatinine after RIR injury in IL-11R WT mice. However, IL-11R deficiency or IL-11 neutralizing antibody prevented the renal protective effects of isoflurane post-conditioning. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Mouse Assay, Injection

    Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in human proximal tubule (HK-2) cells A and B. IL-11 mRNA measured with reverse transcription polymerase chain reaction (RTPCR) in HK-2 cells treated with 0-2.5% isoflurane for 6 h (A. N = 6) or 2.5% isoflurane for 0-6 h (B, N = 6). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was quantified to normalize lane loading. Data are presented as means ± SD. * P

    Journal: Anesthesiology

    Article Title: Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice

    doi: 10.1097/ALN.0b013e3182a950da

    Figure Lengend Snippet: Isoflurane induces interleukin (IL)-11 messenger ribonucleic acid (mRNA) and protein synthesis in human proximal tubule (HK-2) cells A and B. IL-11 mRNA measured with reverse transcription polymerase chain reaction (RTPCR) in HK-2 cells treated with 0-2.5% isoflurane for 6 h (A. N = 6) or 2.5% isoflurane for 0-6 h (B, N = 6). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was quantified to normalize lane loading. Data are presented as means ± SD. * P

    Article Snippet: The inlet port was connected to a vaporizer (Datex-Ohmeda, GE Healthcare, Oklahoma City, OK) to deliver isoflurane (Abbott Laboratories, North Chicago, IL) mixed with 95% air and 5% carbon dioxide (carrier gas) at 10 L/min.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    The effects of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions in HL-1 cells. The cDNA ratios for each subunit used for transfection were the same for those used in . (A) Representative current traces of 2KCNQ1+KCNE1

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The effects of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions in HL-1 cells. The cDNA ratios for each subunit used for transfection were the same for those used in . (A) Representative current traces of 2KCNQ1+KCNE1

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Mutagenesis, Transfection

    Summary of the inhibitory effects of isoflurane under heterozygous conditions and the impact of A341V on current density. (A) Summary of the inhibitory effects of isoflurane on 2KCNQ1+KCNE1 and A341V+KCNQ1+KCNE1 at +60 mV. Similar to the homozygous conditions

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Summary of the inhibitory effects of isoflurane under heterozygous conditions and the impact of A341V on current density. (A) Summary of the inhibitory effects of isoflurane on 2KCNQ1+KCNE1 and A341V+KCNQ1+KCNE1 at +60 mV. Similar to the homozygous conditions

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    Effects on isoflurane on whole-cell potassium currents (IKs) fusion proteins. A. Schematic of the fusion proteins. In the MK24 protein, the C-terminus of KCNE1 was linked to the N-terminus of KCNQ1, and in the MKK44, the C-terminus of KCNE1 was linked

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Effects on isoflurane on whole-cell potassium currents (IKs) fusion proteins. A. Schematic of the fusion proteins. In the MK24 protein, the C-terminus of KCNE1 was linked to the N-terminus of KCNQ1, and in the MKK44, the C-terminus of KCNE1 was linked

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    The impact of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions. Whole-cell currents were monitored in HEK293 cells expressing A341V+KCNQ1+KCNE1 (2.4 μg of complementary DNA [cDNA] for each subunit) and compared to those

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The impact of isoflurane on wild-type IKs and A341V mutant under heterozygous conditions. Whole-cell currents were monitored in HEK293 cells expressing A341V+KCNQ1+KCNE1 (2.4 μg of complementary DNA [cDNA] for each subunit) and compared to those

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Mutagenesis, Expressing

    Role of F340 in determining anesthetic sensitivity. The inhibitory effects of isoflurane (0.5 mM) were determined in two KCNQ1 mutants, F340A and F340C, as shown in panels (A) and (B), respectively. The results are summarized in (C). Current inhibition

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Role of F340 in determining anesthetic sensitivity. The inhibitory effects of isoflurane (0.5 mM) were determined in two KCNQ1 mutants, F340A and F340C, as shown in panels (A) and (B), respectively. The results are summarized in (C). Current inhibition

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Inhibition

    Simulation of the cardiac action potential using the ten Tusscher model of human ventricular myocyte. (A) Simulated action potential under control condition (wild-type IKs) in the absence and presence (dashed lines) of isoflurane. (B) Simulated action potential

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Simulation of the cardiac action potential using the ten Tusscher model of human ventricular myocyte. (A) Simulated action potential under control condition (wild-type IKs) in the absence and presence (dashed lines) of isoflurane. (B) Simulated action potential

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HL-1 cardiomyocytes

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HL-1 cardiomyocytes

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    The impact of isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in a cardiac cell line, HL-1 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in control and in the presence of isoflurane is shown. The voltage protocol is as depicted in

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The impact of isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in a cardiac cell line, HL-1 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in control and in the presence of isoflurane is shown. The voltage protocol is as depicted in

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    The effect of isoflurane on KCNQ1 expressed alone in HL-1 cells. (A) Representative current traces of KCNQ1 are shown in the absence and presence of 0.5 mM isoflurane. (B) The effects of isoflurane on the corresponding voltage-dependence of activation

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The effect of isoflurane on KCNQ1 expressed alone in HL-1 cells. (A) Representative current traces of KCNQ1 are shown in the absence and presence of 0.5 mM isoflurane. (B) The effects of isoflurane on the corresponding voltage-dependence of activation

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Activation Assay

    The impact of isoflurane on wild-type IKs (KCNQ1+KCNE1) and A341V mutant in HEK293 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in the absence and presence of isoflurane (0.54 ± 0.05 mM; equivalent to 1.14 vol % at 22 °C)

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: The impact of isoflurane on wild-type IKs (KCNQ1+KCNE1) and A341V mutant in HEK293 cells. (A) Representative whole-cell current traces of KCNQ1+KCNE1 in the absence and presence of isoflurane (0.54 ± 0.05 mM; equivalent to 1.14 vol % at 22 °C)

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques: Mutagenesis

    Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HEK293 Cells

    Journal: Anesthesiology

    Article Title: Enhanced Effects of Isoflurane on the Long QT Syndrome 1 associated A341V Mutant

    doi: 10.1097/ALN.0000000000000583

    Figure Lengend Snippet: Effects of Isoflurane on KCNQ1+KCNE1 and A341V+KCNE1 in HEK293 Cells

    Article Snippet: Isoflurane (Baxter Healthcare, Deerfield, IL) was dispersed in the external solution, kept in glass-syringe reservoirs to ensure a constant concentration, and delivered to the recording chamber by using syringe pumps at a rate of 90 ml/h, followed by washing out with external solution at a rate of 90 ml/h.

    Techniques:

    Physiological impact of anesthesia as a function of age. ( A ) Baseline blood glucose in mice at 7 (neonatal), 23 (2 days post-weaning), and 26 days of age. ( B ) Baseline blood ß-hydroxybutyrate in mice at 7 (neonatal), 23 (2 days post-weaning), and 26 days of age. ( C ) Respiratory rate of P23 mice as a function of time exposed to 1.5% isoflurane. ANOVA p-value

    Journal: PLoS ONE

    Article Title: Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

    doi: 10.1371/journal.pone.0213543

    Figure Lengend Snippet: Physiological impact of anesthesia as a function of age. ( A ) Baseline blood glucose in mice at 7 (neonatal), 23 (2 days post-weaning), and 26 days of age. ( B ) Baseline blood ß-hydroxybutyrate in mice at 7 (neonatal), 23 (2 days post-weaning), and 26 days of age. ( C ) Respiratory rate of P23 mice as a function of time exposed to 1.5% isoflurane. ANOVA p-value

    Article Snippet: Isoflurane (Piramal Critical Care Inc, NDC66794-013-25) was provided at 1.5% using a routinely maintained and tested isoflurane vaporizer (Patterson Veterinary) at a flow-rate of 3–4 liters/min through a humidifier in-line.

    Techniques: Mouse Assay

    Physiological impact of equipotent sevoflurane or low dose isoflurane in neonatal mice. ( A ) Loss of righting reflex by dose of sevoflurane in P7 neonatal mice. EC50 for sevoflurane was found to be ~2.6% (95% CI = 2.44%-2.73%). Equipotent EC95 for isoflurane and sevoflurane were ~1.5% and ~3.5%, respectively (see panel F). The dashed vertical line marks 3.5%, the concentration used for data in panels B-E. ( B ) Respiratory rate of P7 neonatal mice as a function of time exposed to 3.5% sevoflurane. ANOVA p-value

    Journal: PLoS ONE

    Article Title: Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

    doi: 10.1371/journal.pone.0213543

    Figure Lengend Snippet: Physiological impact of equipotent sevoflurane or low dose isoflurane in neonatal mice. ( A ) Loss of righting reflex by dose of sevoflurane in P7 neonatal mice. EC50 for sevoflurane was found to be ~2.6% (95% CI = 2.44%-2.73%). Equipotent EC95 for isoflurane and sevoflurane were ~1.5% and ~3.5%, respectively (see panel F). The dashed vertical line marks 3.5%, the concentration used for data in panels B-E. ( B ) Respiratory rate of P7 neonatal mice as a function of time exposed to 3.5% sevoflurane. ANOVA p-value

    Article Snippet: Isoflurane (Piramal Critical Care Inc, NDC66794-013-25) was provided at 1.5% using a routinely maintained and tested isoflurane vaporizer (Patterson Veterinary) at a flow-rate of 3–4 liters/min through a humidifier in-line.

    Techniques: Mouse Assay, Concentration Assay

    Cognitive outcomes in anesthesia exposed P7 neonatal mice. ( A ) Barnes maze setup and Barnes maze testing paradigm (see Methods for details and specifications). ( B ) Timeline of anesthesia treatment and Barnes maze assessment and anesthesia paradigms explored in this study. ( C ) Barnes maze paradigm utilized in this study. ( D ) Composite data from all mice from all treatment cohorts. ( E, H ) Assessment of female ( E ) and male ( H ) animals treated with 2 hours of anesthesia with 1.5% isoflurane mice compared to mock treated controls (see Methods ). ( F, I ) Assessment of female ( F ) and male ( I ) animals treated with 4 hours of anesthesia with 1.5% isoflurane or treated 2 hours of 1.5% isoflurane daily for three days compared to mock treated controls (see Methods ). ( G, J ) Assessment of female ( G ) and male ( J ) animals given mock treatment compared to animals receiving no handling (aside from routine animal maintenance) prior to behavioral assessment (see Methods ). Animal numbers are listed in every panel; in all panels data points represent the mean, error bars represent SEM. Row by row comparisons by pairwise t-tests yielded no significant differences (by p-value

    Journal: PLoS ONE

    Article Title: Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

    doi: 10.1371/journal.pone.0213543

    Figure Lengend Snippet: Cognitive outcomes in anesthesia exposed P7 neonatal mice. ( A ) Barnes maze setup and Barnes maze testing paradigm (see Methods for details and specifications). ( B ) Timeline of anesthesia treatment and Barnes maze assessment and anesthesia paradigms explored in this study. ( C ) Barnes maze paradigm utilized in this study. ( D ) Composite data from all mice from all treatment cohorts. ( E, H ) Assessment of female ( E ) and male ( H ) animals treated with 2 hours of anesthesia with 1.5% isoflurane mice compared to mock treated controls (see Methods ). ( F, I ) Assessment of female ( F ) and male ( I ) animals treated with 4 hours of anesthesia with 1.5% isoflurane or treated 2 hours of 1.5% isoflurane daily for three days compared to mock treated controls (see Methods ). ( G, J ) Assessment of female ( G ) and male ( J ) animals given mock treatment compared to animals receiving no handling (aside from routine animal maintenance) prior to behavioral assessment (see Methods ). Animal numbers are listed in every panel; in all panels data points represent the mean, error bars represent SEM. Row by row comparisons by pairwise t-tests yielded no significant differences (by p-value

    Article Snippet: Isoflurane (Piramal Critical Care Inc, NDC66794-013-25) was provided at 1.5% using a routinely maintained and tested isoflurane vaporizer (Patterson Veterinary) at a flow-rate of 3–4 liters/min through a humidifier in-line.

    Techniques: Mouse Assay

    Metabolic recovery timeframe in P7 neonates anesthetized for 2 hours. ( A ) P7 mouse blood glucose concentration at baseline, at 2 hours of anesthesia with 1.5% isoflurane, and at 1 to 4 hours of recovery from 2 hours of anesthesia (baseline and 1-hour timepoints also in Fig 1 ). ANOVA p-value

    Journal: PLoS ONE

    Article Title: Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

    doi: 10.1371/journal.pone.0213543

    Figure Lengend Snippet: Metabolic recovery timeframe in P7 neonates anesthetized for 2 hours. ( A ) P7 mouse blood glucose concentration at baseline, at 2 hours of anesthesia with 1.5% isoflurane, and at 1 to 4 hours of recovery from 2 hours of anesthesia (baseline and 1-hour timepoints also in Fig 1 ). ANOVA p-value

    Article Snippet: Isoflurane (Piramal Critical Care Inc, NDC66794-013-25) was provided at 1.5% using a routinely maintained and tested isoflurane vaporizer (Patterson Veterinary) at a flow-rate of 3–4 liters/min through a humidifier in-line.

    Techniques: Concentration Assay

    Anesthesia induced apoptosis in P7 neonatal mice. ( A ) Western blot probing for cleaved caspase-3 in brain lysates from control P7 mice (unexposed) and P7 mice exposed to 1.5% isoflurane for 4 hours. **p

    Journal: PLoS ONE

    Article Title: Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

    doi: 10.1371/journal.pone.0213543

    Figure Lengend Snippet: Anesthesia induced apoptosis in P7 neonatal mice. ( A ) Western blot probing for cleaved caspase-3 in brain lysates from control P7 mice (unexposed) and P7 mice exposed to 1.5% isoflurane for 4 hours. **p

    Article Snippet: Isoflurane (Piramal Critical Care Inc, NDC66794-013-25) was provided at 1.5% using a routinely maintained and tested isoflurane vaporizer (Patterson Veterinary) at a flow-rate of 3–4 liters/min through a humidifier in-line.

    Techniques: Mouse Assay, Western Blot

    Physiological impact of prolonged anesthesia in P7 neonatal mice. ( A ) Experimental anesthesia setup. P7 neonatal mice were exposed to 1.5% isoflurane in 100% oxygen humidified air in a Plexiglass chamber, with gasses and chamber maintained at 38° C. ( B ) Respiratory rate in P7 mice as a function of time exposed to 1.5% isoflurane. Error bars depict standard error of the mean (SEM), dotted center lines depict non-linear best fit curve, dashed outer lines depict 95% confidence intervals (see Methods ). n = 16 and 3 for 1.5% isoflurane and 1% isoflurane, respectively. ( C ) Comparison of respiratory rates in unexposed mice and mice anesthetized with 1.5% isoflurane for 2 or 4 hours ANOVA p-value

    Journal: PLoS ONE

    Article Title: Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

    doi: 10.1371/journal.pone.0213543

    Figure Lengend Snippet: Physiological impact of prolonged anesthesia in P7 neonatal mice. ( A ) Experimental anesthesia setup. P7 neonatal mice were exposed to 1.5% isoflurane in 100% oxygen humidified air in a Plexiglass chamber, with gasses and chamber maintained at 38° C. ( B ) Respiratory rate in P7 mice as a function of time exposed to 1.5% isoflurane. Error bars depict standard error of the mean (SEM), dotted center lines depict non-linear best fit curve, dashed outer lines depict 95% confidence intervals (see Methods ). n = 16 and 3 for 1.5% isoflurane and 1% isoflurane, respectively. ( C ) Comparison of respiratory rates in unexposed mice and mice anesthetized with 1.5% isoflurane for 2 or 4 hours ANOVA p-value

    Article Snippet: Isoflurane (Piramal Critical Care Inc, NDC66794-013-25) was provided at 1.5% using a routinely maintained and tested isoflurane vaporizer (Patterson Veterinary) at a flow-rate of 3–4 liters/min through a humidifier in-line.

    Techniques: Mouse Assay