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  • 90
    ProSci Incorporated irak m
    Irak M, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irak m/product/ProSci Incorporated
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    93
    Rockland Immunochemicals irak3
    Primers used for qRT-PCR analysis.
    Irak3, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irak3/product/Rockland Immunochemicals
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    93
    Cell Signaling Technology Inc anti irak m
    Expression of TNF-α, IL-6, IL-10 <t>and</t> <t>IRAK-M</t> in CLP+LPS and control mice. The concentration of (A) TNF-α, (B) IL-6 and (C) IL-10 in the supernatant of the culture medium of PBMCs was analyzed using ELISAs, and (D) IRAK-M expression was analyzed using reverse transcription-quantitative polymerase chain reaction in PBMCs from CLP-LPS mice (n=6) or control mice (n=6). The results are presented as the mean ± standard deviation. *P<0.05 as indicated. CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; con, mice that underwent sham surgery followed by LPS challenge.
    Anti Irak M, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti irak m/product/Cell Signaling Technology Inc
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    93
    Santa Cruz Biotechnology irak m sirna
    A ., B . THP-1 macrophages were infected with lentiviral particles pseudotyped with MERS wild type (wt) or the D510A mutant S glycoprotein (D510A) of MERS-CoV, or left uninfected (mock) in the presence or absence of the DPP4 inhibitor sitagliptin or <t>siRNA</t> targeting DPP4. 24 hours post-infection RNA was isolated and the levels of <t>IRAK-M</t> A ., B . and PPARγ C ., D . and IL-10 E ., F . were measured. ns: not significant, * p < 0.05, ** p < 0.01
    Irak M Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irak m sirna/product/Santa Cruz Biotechnology
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    88
    Santa Cruz Biotechnology goat anti irak m antibody
    * p<0.05 relative to the ratio of fold increase in the mRNA levels of cells primed only in control media in absence of diabetic drugs. (B) Flow cytometry of glyburide-treated PBMC showed increased expression <t>of</t> <t>IRAK-M</t> in CD-14 positive monocytes following stimulation with purified Bp antigen. The cells were surface labeled with anti-CD14-V450 antibody, permeabilized and then labeled with primary goat anti-IRAK-M antibody. Secondary labeling with donkey anti-goat-FITC antibody was performed and cells were analyzed by flow cytometry. Result from a representative set of experiment is shown.
    Goat Anti Irak M Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Primers used for qRT-PCR analysis.

    Journal: PLoS ONE

    Article Title: PPAR Agonist-Induced Reduction of Mcp1 in Atherosclerotic Plaques of Obese, Insulin-Resistant Mice Depends on Adiponectin-Induced Irak3 Expression

    doi: 10.1371/journal.pone.0062253

    Figure Lengend Snippet: Primers used for qRT-PCR analysis.

    Article Snippet: To detect protein levels, membranes were incubated with primary antibodies against β-actin (Cell Signaling Technology) and Irak3 (Rockland Immunochemicals).

    Techniques:

    ( A ) Representative Mac-3 staining of aortic sinus plaques of placebo-, fenofibrate- and rosiglitazone-treated DKO mice at 24 weeks. ( B ) Gene expression in the aorta was analyzed by measuring relative RNA levels using qRT-PCR for Tnfα , IL6 , Mcp1 and Irak3 . Data are means ± SEM. Scale bar = 500 µm. * P <0.05, ** P <0.01 and *** P <0.001 DKO compared with C57BL/6 J mice; $$ P <0.01 and $$$ P <0.001 PPAR agonist-treated compared with placebo-treated DKO mice; £ P <0.05, ££ P <0.01 and £££ P <0.001 rosiglitazone-treated compared with fenofibrate-treated DKO mice.

    Journal: PLoS ONE

    Article Title: PPAR Agonist-Induced Reduction of Mcp1 in Atherosclerotic Plaques of Obese, Insulin-Resistant Mice Depends on Adiponectin-Induced Irak3 Expression

    doi: 10.1371/journal.pone.0062253

    Figure Lengend Snippet: ( A ) Representative Mac-3 staining of aortic sinus plaques of placebo-, fenofibrate- and rosiglitazone-treated DKO mice at 24 weeks. ( B ) Gene expression in the aorta was analyzed by measuring relative RNA levels using qRT-PCR for Tnfα , IL6 , Mcp1 and Irak3 . Data are means ± SEM. Scale bar = 500 µm. * P <0.05, ** P <0.01 and *** P <0.001 DKO compared with C57BL/6 J mice; $$ P <0.01 and $$$ P <0.001 PPAR agonist-treated compared with placebo-treated DKO mice; £ P <0.05, ££ P <0.01 and £££ P <0.001 rosiglitazone-treated compared with fenofibrate-treated DKO mice.

    Article Snippet: To detect protein levels, membranes were incubated with primary antibodies against β-actin (Cell Signaling Technology) and Irak3 (Rockland Immunochemicals).

    Techniques: Staining, Expressing, Quantitative RT-PCR

    ( A ) Soluble Mcp1 protein levels in DKO BMDM exposed to 50 µM fenofibrate, 10 µM rosiglitazone or 5 µM GW9662 for 24 hours as determined by ELISA. Data are means ± SEM; n = 16 from three different mice. $$ P <0.01 compared with fenofibrate-treated BMDM. ( B ) Irak3 RNA and protein levels of DKO BMDM exposed to 1 or 10 µg/mL globular adiponectin for 24 hours as determined by qRT-PCR and Western blotting. Data are means ± SEM; n = 6. *** P <0.001 compared with DKO BMDM; $ P <0.05 and $$ P <0.01 compared with DKO BMDM exposed to 1 µg/mL globular adiponectin. ( C ) Soluble Mcp1 protein levels (n = 18 from three different mice), NFκB p50 DNA binding activity (n = 8 from two different mice) and mROS production (n = 6) in IRAK3 −/− BMDM exposed to 50 µM fenofibrate or 10 µM rosiglitazone for 24 hours as determined by ELISA and flow cytometry. Data are means ± SEM. * P <0.05, ** P <0.01 and *** P <0.001 compared with C57BL/6 J BMDM; $ P <0.05 and $$$ P <0.001 compared with IRAK3 −/− BMDM; £££ P <0.001 compared with fenofibrate-treated BMDM. Abbreviations: BMDM, bone marrow-derived macrophages; mROS, mitochondrial reactive oxygen species.

    Journal: PLoS ONE

    Article Title: PPAR Agonist-Induced Reduction of Mcp1 in Atherosclerotic Plaques of Obese, Insulin-Resistant Mice Depends on Adiponectin-Induced Irak3 Expression

    doi: 10.1371/journal.pone.0062253

    Figure Lengend Snippet: ( A ) Soluble Mcp1 protein levels in DKO BMDM exposed to 50 µM fenofibrate, 10 µM rosiglitazone or 5 µM GW9662 for 24 hours as determined by ELISA. Data are means ± SEM; n = 16 from three different mice. $$ P <0.01 compared with fenofibrate-treated BMDM. ( B ) Irak3 RNA and protein levels of DKO BMDM exposed to 1 or 10 µg/mL globular adiponectin for 24 hours as determined by qRT-PCR and Western blotting. Data are means ± SEM; n = 6. *** P <0.001 compared with DKO BMDM; $ P <0.05 and $$ P <0.01 compared with DKO BMDM exposed to 1 µg/mL globular adiponectin. ( C ) Soluble Mcp1 protein levels (n = 18 from three different mice), NFκB p50 DNA binding activity (n = 8 from two different mice) and mROS production (n = 6) in IRAK3 −/− BMDM exposed to 50 µM fenofibrate or 10 µM rosiglitazone for 24 hours as determined by ELISA and flow cytometry. Data are means ± SEM. * P <0.05, ** P <0.01 and *** P <0.001 compared with C57BL/6 J BMDM; $ P <0.05 and $$$ P <0.001 compared with IRAK3 −/− BMDM; £££ P <0.001 compared with fenofibrate-treated BMDM. Abbreviations: BMDM, bone marrow-derived macrophages; mROS, mitochondrial reactive oxygen species.

    Article Snippet: To detect protein levels, membranes were incubated with primary antibodies against β-actin (Cell Signaling Technology) and Irak3 (Rockland Immunochemicals).

    Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Binding Assay, Activity Assay, Flow Cytometry, Derivative Assay

    Plaque volume was determined by measuring lipid (oil red O)-stained surfaces in subsequent sections; macrophages were stained with anti-Mac-3 antibody. Gene expression in the aorta was analyzed by measuring relative RNA levels using qRT-PCR for Pparγ , Mcp1, Irak3 and Adipoq . Data are means ± SEM. ** P <0.01 and *** P <0.001 HFD-fed compared with SD-fed LDL-receptor deficient mice. Abbreviations: HFD, high fat diet; SD, standard diet.

    Journal: PLoS ONE

    Article Title: PPAR Agonist-Induced Reduction of Mcp1 in Atherosclerotic Plaques of Obese, Insulin-Resistant Mice Depends on Adiponectin-Induced Irak3 Expression

    doi: 10.1371/journal.pone.0062253

    Figure Lengend Snippet: Plaque volume was determined by measuring lipid (oil red O)-stained surfaces in subsequent sections; macrophages were stained with anti-Mac-3 antibody. Gene expression in the aorta was analyzed by measuring relative RNA levels using qRT-PCR for Pparγ , Mcp1, Irak3 and Adipoq . Data are means ± SEM. ** P <0.01 and *** P <0.001 HFD-fed compared with SD-fed LDL-receptor deficient mice. Abbreviations: HFD, high fat diet; SD, standard diet.

    Article Snippet: To detect protein levels, membranes were incubated with primary antibodies against β-actin (Cell Signaling Technology) and Irak3 (Rockland Immunochemicals).

    Techniques: Staining, Expressing, Quantitative RT-PCR

    The schematic draw demonstrates the anti-atherosclerotic properties of the PPARγ agonist rosiglitazone. Treatment with rosiglitazone improves the adipocyte function characterized by a decrease in adipocyte size, a reduction in adipose tissue macrophages and an increased expression of anti-inflammatory adiponectin. The increase in blood adiponectin and de novo adiponectin production in atherosclerotic lesions is necessary for the upregulation of Irak3 in plaque macrophages, which is crucial for the indirect rosiglitazone-mediated decrease in Mcp1 secretion. Abbreviations: Mφ, macrophages; ROS, reactive oxygen species.

    Journal: PLoS ONE

    Article Title: PPAR Agonist-Induced Reduction of Mcp1 in Atherosclerotic Plaques of Obese, Insulin-Resistant Mice Depends on Adiponectin-Induced Irak3 Expression

    doi: 10.1371/journal.pone.0062253

    Figure Lengend Snippet: The schematic draw demonstrates the anti-atherosclerotic properties of the PPARγ agonist rosiglitazone. Treatment with rosiglitazone improves the adipocyte function characterized by a decrease in adipocyte size, a reduction in adipose tissue macrophages and an increased expression of anti-inflammatory adiponectin. The increase in blood adiponectin and de novo adiponectin production in atherosclerotic lesions is necessary for the upregulation of Irak3 in plaque macrophages, which is crucial for the indirect rosiglitazone-mediated decrease in Mcp1 secretion. Abbreviations: Mφ, macrophages; ROS, reactive oxygen species.

    Article Snippet: To detect protein levels, membranes were incubated with primary antibodies against β-actin (Cell Signaling Technology) and Irak3 (Rockland Immunochemicals).

    Techniques: Expressing

    Expression of TNF-α, IL-6, IL-10 and IRAK-M in CLP+LPS and control mice. The concentration of (A) TNF-α, (B) IL-6 and (C) IL-10 in the supernatant of the culture medium of PBMCs was analyzed using ELISAs, and (D) IRAK-M expression was analyzed using reverse transcription-quantitative polymerase chain reaction in PBMCs from CLP-LPS mice (n=6) or control mice (n=6). The results are presented as the mean ± standard deviation. *P<0.05 as indicated. CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; con, mice that underwent sham surgery followed by LPS challenge.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis

    doi: 10.3892/etm.2017.5549

    Figure Lengend Snippet: Expression of TNF-α, IL-6, IL-10 and IRAK-M in CLP+LPS and control mice. The concentration of (A) TNF-α, (B) IL-6 and (C) IL-10 in the supernatant of the culture medium of PBMCs was analyzed using ELISAs, and (D) IRAK-M expression was analyzed using reverse transcription-quantitative polymerase chain reaction in PBMCs from CLP-LPS mice (n=6) or control mice (n=6). The results are presented as the mean ± standard deviation. *P<0.05 as indicated. CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; con, mice that underwent sham surgery followed by LPS challenge.

    Article Snippet: Membranes were blocked using 5% non-fat milk, at room temperature for 1 h. Following this, membranes were incubated with anti-IRAK-M (dilution, 1:1,000; cat. no. 4369T) and anti-GAPDH (dilution, 1:1,000; cat. no. 5174T; both from Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C overnight.

    Techniques: Expressing, Concentration Assay, Real-time Polymerase Chain Reaction, Standard Deviation, Ligation

    Expression of TNF-α, IL-6, IL-10 and IRAK-M following IRAK-M downregulation in PMBCs. The level of IRAK-M (A) mRNA and (B) protein was analyzed using reverse transcription-polymerase chain reaction and western blotting analyses, respectively, following transfection of PMBCs with IRAK-M or control siRNA. The concentration of (C) TNF-α, (D) IL-6 and (E) IL-10 in the supernatant of culture medium following transfection with siRNA was analyzed using ELISAs. Data are presented as the mean ± standard deviation (n=6). *P<0.05 as indicated. TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; PMBCs, peripheral mononuclear blood cells; siRNA, small interfering RNA; CLP, cecal ligation puncture; si-PMBCs, PMBCs derived from CLP-LPS mice transfected with IRAK-M-siRNA; con, PMBCs from CLP-LPS mice transfected with control-siRNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis

    doi: 10.3892/etm.2017.5549

    Figure Lengend Snippet: Expression of TNF-α, IL-6, IL-10 and IRAK-M following IRAK-M downregulation in PMBCs. The level of IRAK-M (A) mRNA and (B) protein was analyzed using reverse transcription-polymerase chain reaction and western blotting analyses, respectively, following transfection of PMBCs with IRAK-M or control siRNA. The concentration of (C) TNF-α, (D) IL-6 and (E) IL-10 in the supernatant of culture medium following transfection with siRNA was analyzed using ELISAs. Data are presented as the mean ± standard deviation (n=6). *P<0.05 as indicated. TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; PMBCs, peripheral mononuclear blood cells; siRNA, small interfering RNA; CLP, cecal ligation puncture; si-PMBCs, PMBCs derived from CLP-LPS mice transfected with IRAK-M-siRNA; con, PMBCs from CLP-LPS mice transfected with control-siRNA.

    Article Snippet: Membranes were blocked using 5% non-fat milk, at room temperature for 1 h. Following this, membranes were incubated with anti-IRAK-M (dilution, 1:1,000; cat. no. 4369T) and anti-GAPDH (dilution, 1:1,000; cat. no. 5174T; both from Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C overnight.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection, Concentration Assay, Standard Deviation, Small Interfering RNA, Ligation, Derivative Assay

    Survival rate and serum TNF-α, IL-6 and IL-10 levels in mice administered with PBMCs transfected with IRAK-M siRNA. (A) The survival rate and level of (B) TNF-α, (C) IL-6 and (D) IL-10 levels in the serum of CLP-LPS mice injected with PBMCs transfected with IRAK-M siRNA (n=6) or control siRNA (n=6) compared with the sham+LPS and CLP+LPS mice that were not injected with PBMCs. The results are presented as the mean ± standard deviation. *P<0.05 as indicated. TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; PMBCs, peripheral mononuclear blood cells; siRNA, small interfering RNA; CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; CLP-LPS-si, mice that underwent CLP surgery followed by LPS challenge and injection with PBMCs transfected with IRAK-M siRNA; CLP-LPS-con, mice that underwent CLP surgery followed by LPS challenge and injection of PMBCs transfected with control siRNA; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; Sham+LPS, mice that underwent sham surgery followed by LPS challenge only.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis

    doi: 10.3892/etm.2017.5549

    Figure Lengend Snippet: Survival rate and serum TNF-α, IL-6 and IL-10 levels in mice administered with PBMCs transfected with IRAK-M siRNA. (A) The survival rate and level of (B) TNF-α, (C) IL-6 and (D) IL-10 levels in the serum of CLP-LPS mice injected with PBMCs transfected with IRAK-M siRNA (n=6) or control siRNA (n=6) compared with the sham+LPS and CLP+LPS mice that were not injected with PBMCs. The results are presented as the mean ± standard deviation. *P<0.05 as indicated. TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; PMBCs, peripheral mononuclear blood cells; siRNA, small interfering RNA; CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; CLP-LPS-si, mice that underwent CLP surgery followed by LPS challenge and injection with PBMCs transfected with IRAK-M siRNA; CLP-LPS-con, mice that underwent CLP surgery followed by LPS challenge and injection of PMBCs transfected with control siRNA; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; Sham+LPS, mice that underwent sham surgery followed by LPS challenge only.

    Article Snippet: Membranes were blocked using 5% non-fat milk, at room temperature for 1 h. Following this, membranes were incubated with anti-IRAK-M (dilution, 1:1,000; cat. no. 4369T) and anti-GAPDH (dilution, 1:1,000; cat. no. 5174T; both from Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C overnight.

    Techniques: Transfection, Injection, Standard Deviation, Small Interfering RNA, Ligation

    Treatment of septic rats with PBMCs transfected with IRAK-M siRNA led to T cell activation and a decrease in apoptosis. (A and B) Representative flow cytometry plots showing the percentage of (C) CD4+, (D) CD8+, (E) apoptotic CD4+ and (F) apoptotic CD8+ T cells in CLP+LPS mice administered with IRAK-M-downregulated PBMCs (n=6), CLP+LPS mice administered with control PBMCs (n=6), CLP+LPS mice (n=6) and Sham+LPS mice (n=6). The results are presented as the mean ± standard deviation. *P<0.05, as indicated. PMBCs, peripheral mononuclear blood cells; IRAK-M, interleukin-1 receptor-associated kinase 3; siRNA, small interfering RNA; CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; CLP-LPS-si, mice that underwent CLP surgery followed by LPS challenge and injection with PBMCs transfected with IRAK-M siRNA; CLP-LPS-con, mice that underwent CLP surgery followed by LPS challenge and injection of PMBCs transfected with control siRNA; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; Sham+LPS, mice that underwent sham surgery followed by LPS challenge only.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis

    doi: 10.3892/etm.2017.5549

    Figure Lengend Snippet: Treatment of septic rats with PBMCs transfected with IRAK-M siRNA led to T cell activation and a decrease in apoptosis. (A and B) Representative flow cytometry plots showing the percentage of (C) CD4+, (D) CD8+, (E) apoptotic CD4+ and (F) apoptotic CD8+ T cells in CLP+LPS mice administered with IRAK-M-downregulated PBMCs (n=6), CLP+LPS mice administered with control PBMCs (n=6), CLP+LPS mice (n=6) and Sham+LPS mice (n=6). The results are presented as the mean ± standard deviation. *P<0.05, as indicated. PMBCs, peripheral mononuclear blood cells; IRAK-M, interleukin-1 receptor-associated kinase 3; siRNA, small interfering RNA; CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; CLP-LPS-si, mice that underwent CLP surgery followed by LPS challenge and injection with PBMCs transfected with IRAK-M siRNA; CLP-LPS-con, mice that underwent CLP surgery followed by LPS challenge and injection of PMBCs transfected with control siRNA; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; Sham+LPS, mice that underwent sham surgery followed by LPS challenge only.

    Article Snippet: Membranes were blocked using 5% non-fat milk, at room temperature for 1 h. Following this, membranes were incubated with anti-IRAK-M (dilution, 1:1,000; cat. no. 4369T) and anti-GAPDH (dilution, 1:1,000; cat. no. 5174T; both from Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C overnight.

    Techniques: Transfection, Activation Assay, Flow Cytometry, Standard Deviation, Small Interfering RNA, Ligation, Injection

    A ., B . THP-1 macrophages were infected with lentiviral particles pseudotyped with MERS wild type (wt) or the D510A mutant S glycoprotein (D510A) of MERS-CoV, or left uninfected (mock) in the presence or absence of the DPP4 inhibitor sitagliptin or siRNA targeting DPP4. 24 hours post-infection RNA was isolated and the levels of IRAK-M A ., B . and PPARγ C ., D . and IL-10 E ., F . were measured. ns: not significant, * p < 0.05, ** p < 0.01

    Journal: Oncotarget

    Article Title: Middle east respiratory syndrome corona virus spike glycoprotein suppresses macrophage responses via DPP4-mediated induction of IRAK-M and PPARγ

    doi: 10.18632/oncotarget.14754

    Figure Lengend Snippet: A ., B . THP-1 macrophages were infected with lentiviral particles pseudotyped with MERS wild type (wt) or the D510A mutant S glycoprotein (D510A) of MERS-CoV, or left uninfected (mock) in the presence or absence of the DPP4 inhibitor sitagliptin or siRNA targeting DPP4. 24 hours post-infection RNA was isolated and the levels of IRAK-M A ., B . and PPARγ C ., D . and IL-10 E ., F . were measured. ns: not significant, * p < 0.05, ** p < 0.01

    Article Snippet: Cells were cultured for 24 hours in the presence of the transfection reagents prior to infection and LPS treatment, the following, pre-validated siRNAs used were: IRAK-M siRNA (sc-39098, Santa-Cruz), PPARγ siRNA (sc-29455, Santa-Cruz), CD26 siRNA (sc-42762, Santa-Cruz) and Silencer Negative Control (SCR) siRNA (AM4611g, Ambion).

    Techniques: Infection, Mutagenesis, Isolation

    * p<0.05 relative to the ratio of fold increase in the mRNA levels of cells primed only in control media in absence of diabetic drugs. (B) Flow cytometry of glyburide-treated PBMC showed increased expression of IRAK-M in CD-14 positive monocytes following stimulation with purified Bp antigen. The cells were surface labeled with anti-CD14-V450 antibody, permeabilized and then labeled with primary goat anti-IRAK-M antibody. Secondary labeling with donkey anti-goat-FITC antibody was performed and cells were analyzed by flow cytometry. Result from a representative set of experiment is shown.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Sulphonylurea Usage in Melioidosis Is Associated with Severe Disease and Suppressed Immune Response

    doi: 10.1371/journal.pntd.0002795

    Figure Lengend Snippet: * p<0.05 relative to the ratio of fold increase in the mRNA levels of cells primed only in control media in absence of diabetic drugs. (B) Flow cytometry of glyburide-treated PBMC showed increased expression of IRAK-M in CD-14 positive monocytes following stimulation with purified Bp antigen. The cells were surface labeled with anti-CD14-V450 antibody, permeabilized and then labeled with primary goat anti-IRAK-M antibody. Secondary labeling with donkey anti-goat-FITC antibody was performed and cells were analyzed by flow cytometry. Result from a representative set of experiment is shown.

    Article Snippet: For cells stained with unlabelled goat anti-IRAK-M antibody, secondary labeling with donkey anti-goat-FITC antibody (Santa Cruz Biotechnology, Dallas, TX) was carried out.

    Techniques: Flow Cytometry, Expressing, Purification, Labeling