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  • 99
    Bio-Rad iq5 instrument
    Iq5 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 645 article reviews
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    89
    Bio-Rad bio rad iq5 instrument
    Bio Rad Iq5 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 343 article reviews
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    88
    Bio-Rad icycler iq5 real time pcr instrument
    Icycler Iq5 Real Time Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad iq5 0 instrument
    Iq5 0 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad iq5 pcr instrument
    Iq5 Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iq5 pcr instrument/product/Bio-Rad
    Average 85 stars, based on 66 article reviews
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    88
    Bio-Rad icycler iq5 instrument
    Icycler Iq5 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icycler iq5 instrument/product/Bio-Rad
    Average 88 stars, based on 60 article reviews
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    99
    Bio-Rad iq5 96 well format real time pcr instrument
    Iq5 96 Well Format Real Time Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iq5 96 well format real time pcr instrument/product/Bio-Rad
    Average 99 stars, based on 56 article reviews
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    85
    Bio-Rad iq5 quantitative pcr instrument
    Iq5 Quantitative Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad iq5 cycler instrument
    Iq5 Cycler Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 23 article reviews
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    91
    Bio-Rad iq5 qrt pcr instrument
    MT1DP regulates its parental gene MT1H level through a ceRNA mechanism. a Cell death analysis through flow cytometry analysis with PI staining in scrambled control and MT1H low cells upon Cd treatment at 20 μmol/L for 3, 6, 12, and 24 h ( n = 3). b <t>qRT-PCR</t> analysis of relative mRNA levels of MT1 family numbers in MT1DP overexpressed HepG2 cells ( n = 3). c qRT-PCR assay of MT1H expression levels in scrambled control and MT1DP low cells ( n = 3). d MT1H mRNA levels in HepG2 cells in response to Cd over time, determined by qRT-PCR ( n = 3). e qRT-PCR determination of MT1H mRNA levels in scrambled control and MT1DP low cells upon Cd treatment at 10 and 20 μmol/L Cd for 24 h ( n = 3). f Exotic FLAG-MT1H CDS + 3ʹ-UTR construct was transfected into scrambled control and MT1DP low cells for 24 h, and cells were treated with Cd at indicated concentrations for 6 h, followed by western blot analysis of FLAG-MT1H. g Endogenous MT1H mRNA levels were knocked down by two sets of selective siRNA molecules in HepG2 cells, and then MT1DP levels were assayed by qRT-PCR analysis ( n = 3)
    Iq5 Qrt Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad iq5 real time qpcr instrument
    MT1DP regulates its parental gene MT1H level through a ceRNA mechanism. a Cell death analysis through flow cytometry analysis with PI staining in scrambled control and MT1H low cells upon Cd treatment at 20 μmol/L for 3, 6, 12, and 24 h ( n = 3). b <t>qRT-PCR</t> analysis of relative mRNA levels of MT1 family numbers in MT1DP overexpressed HepG2 cells ( n = 3). c qRT-PCR assay of MT1H expression levels in scrambled control and MT1DP low cells ( n = 3). d MT1H mRNA levels in HepG2 cells in response to Cd over time, determined by qRT-PCR ( n = 3). e qRT-PCR determination of MT1H mRNA levels in scrambled control and MT1DP low cells upon Cd treatment at 10 and 20 μmol/L Cd for 24 h ( n = 3). f Exotic FLAG-MT1H CDS + 3ʹ-UTR construct was transfected into scrambled control and MT1DP low cells for 24 h, and cells were treated with Cd at indicated concentrations for 6 h, followed by western blot analysis of FLAG-MT1H. g Endogenous MT1H mRNA levels were knocked down by two sets of selective siRNA molecules in HepG2 cells, and then MT1DP levels were assayed by qRT-PCR analysis ( n = 3)
    Iq5 Real Time Qpcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad iq5 real time pcr thermal cycle instrument
    MT1DP regulates its parental gene MT1H level through a ceRNA mechanism. a Cell death analysis through flow cytometry analysis with PI staining in scrambled control and MT1H low cells upon Cd treatment at 20 μmol/L for 3, 6, 12, and 24 h ( n = 3). b <t>qRT-PCR</t> analysis of relative mRNA levels of MT1 family numbers in MT1DP overexpressed HepG2 cells ( n = 3). c qRT-PCR assay of MT1H expression levels in scrambled control and MT1DP low cells ( n = 3). d MT1H mRNA levels in HepG2 cells in response to Cd over time, determined by qRT-PCR ( n = 3). e qRT-PCR determination of MT1H mRNA levels in scrambled control and MT1DP low cells upon Cd treatment at 10 and 20 μmol/L Cd for 24 h ( n = 3). f Exotic FLAG-MT1H CDS + 3ʹ-UTR construct was transfected into scrambled control and MT1DP low cells for 24 h, and cells were treated with Cd at indicated concentrations for 6 h, followed by western blot analysis of FLAG-MT1H. g Endogenous MT1H mRNA levels were knocked down by two sets of selective siRNA molecules in HepG2 cells, and then MT1DP levels were assayed by qRT-PCR analysis ( n = 3)
    Iq5 Real Time Pcr Thermal Cycle Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 20 article reviews
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    85
    Bio-Rad pcr qpcr iq5 instrument
    MT1DP regulates its parental gene MT1H level through a ceRNA mechanism. a Cell death analysis through flow cytometry analysis with PI staining in scrambled control and MT1H low cells upon Cd treatment at 20 μmol/L for 3, 6, 12, and 24 h ( n = 3). b <t>qRT-PCR</t> analysis of relative mRNA levels of MT1 family numbers in MT1DP overexpressed HepG2 cells ( n = 3). c qRT-PCR assay of MT1H expression levels in scrambled control and MT1DP low cells ( n = 3). d MT1H mRNA levels in HepG2 cells in response to Cd over time, determined by qRT-PCR ( n = 3). e qRT-PCR determination of MT1H mRNA levels in scrambled control and MT1DP low cells upon Cd treatment at 10 and 20 μmol/L Cd for 24 h ( n = 3). f Exotic FLAG-MT1H CDS + 3ʹ-UTR construct was transfected into scrambled control and MT1DP low cells for 24 h, and cells were treated with Cd at indicated concentrations for 6 h, followed by western blot analysis of FLAG-MT1H. g Endogenous MT1H mRNA levels were knocked down by two sets of selective siRNA molecules in HepG2 cells, and then MT1DP levels were assayed by qRT-PCR analysis ( n = 3)
    Pcr Qpcr Iq5 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    MT1DP regulates its parental gene MT1H level through a ceRNA mechanism. a Cell death analysis through flow cytometry analysis with PI staining in scrambled control and MT1H low cells upon Cd treatment at 20 μmol/L for 3, 6, 12, and 24 h ( n = 3). b qRT-PCR analysis of relative mRNA levels of MT1 family numbers in MT1DP overexpressed HepG2 cells ( n = 3). c qRT-PCR assay of MT1H expression levels in scrambled control and MT1DP low cells ( n = 3). d MT1H mRNA levels in HepG2 cells in response to Cd over time, determined by qRT-PCR ( n = 3). e qRT-PCR determination of MT1H mRNA levels in scrambled control and MT1DP low cells upon Cd treatment at 10 and 20 μmol/L Cd for 24 h ( n = 3). f Exotic FLAG-MT1H CDS + 3ʹ-UTR construct was transfected into scrambled control and MT1DP low cells for 24 h, and cells were treated with Cd at indicated concentrations for 6 h, followed by western blot analysis of FLAG-MT1H. g Endogenous MT1H mRNA levels were knocked down by two sets of selective siRNA molecules in HepG2 cells, and then MT1DP levels were assayed by qRT-PCR analysis ( n = 3)

    Journal: Cell Discovery

    Article Title: Long non-coding RNA MT1DP shunts the cellular defense to cytotoxicity through crosstalk with MT1H and RhoC in cadmium stress

    doi: 10.1038/s41421-017-0005-y

    Figure Lengend Snippet: MT1DP regulates its parental gene MT1H level through a ceRNA mechanism. a Cell death analysis through flow cytometry analysis with PI staining in scrambled control and MT1H low cells upon Cd treatment at 20 μmol/L for 3, 6, 12, and 24 h ( n = 3). b qRT-PCR analysis of relative mRNA levels of MT1 family numbers in MT1DP overexpressed HepG2 cells ( n = 3). c qRT-PCR assay of MT1H expression levels in scrambled control and MT1DP low cells ( n = 3). d MT1H mRNA levels in HepG2 cells in response to Cd over time, determined by qRT-PCR ( n = 3). e qRT-PCR determination of MT1H mRNA levels in scrambled control and MT1DP low cells upon Cd treatment at 10 and 20 μmol/L Cd for 24 h ( n = 3). f Exotic FLAG-MT1H CDS + 3ʹ-UTR construct was transfected into scrambled control and MT1DP low cells for 24 h, and cells were treated with Cd at indicated concentrations for 6 h, followed by western blot analysis of FLAG-MT1H. g Endogenous MT1H mRNA levels were knocked down by two sets of selective siRNA molecules in HepG2 cells, and then MT1DP levels were assayed by qRT-PCR analysis ( n = 3)

    Article Snippet: Total RNAs were extracted from cells with Trizol reagent (Invitrogen, USA), and then 2–5 μg RNAs were reverse-transcribed into cDNA with M-MLV reverse transcriptase. qPCR was performed on an iQ5 qRT-PCR instrument (Bio-Rad), as described previously , GAPDH was used as a loading control for normalization.

    Techniques: Flow Cytometry, Cytometry, Staining, Quantitative RT-PCR, Expressing, Construct, Transfection, Western Blot

    MT1DP competes for miR-214 with MT1H. a HepG2 cells were transfected with exotic FLAG-MT1H CDS, FLAG-MT1H CDS + 3ʹ-UTR, and FLAG-MT1H 3ʹ-UTR for 24 h, respectively, and then the MT1DP levels were measured by qRT-PCR ( n = 3). b A schematic illustrating the putative target sites for MT1DP and MT1H in competing for miR-214. c – f Levels of MT1DP and MT1H were determined by qRT-PCR in cells transfected with NC-mimic and miR-214 mimic molecules c , d and NC-inhibitor and miR-214 inhibitor molecules e , f ( n = 3), respectively. g, h Relative luciferase activities in HepG2 cells with expression of pGL3-vehicle, pGL3-MT1DP, and pGL3-MT1DP-mutant constructs g and pGL3-vehicle, pGL3-MT1H 3ʹ-UTR, and pGL3-MT1H 3ʹ-UTR-mutant constructs h upon transfection of NC-mimic and miR-214 mimic molecules, measured through the dual-luciferase assay ( n = 3). i Relative enrichment of miR-214 in the pull-down lysates from cells using MS2-MT1DP and MS2-MT1DP mutant RNAs, respectively, examined by qRT-PCR assay ( n = 3). Fold changes were normalized to U6 RNA levels. j The MT1H levels in HepG2 cells transfected with synthesized molecules of vehicle control, MT1DP, miR-214 and MT1DP + miR-214, detected by qRT-PCR assay ( n = 3). k Western blot analysis of FLAG-MT1H in HepG2 cells. Cells were pre-transfected with FLAG-MT1H CDS + 3ʹ-UTR for 24 h, and were further transfected with synthesized molecules of vehicle control, MT1DP, miR-214 and MT1DP + miRNA-214 for another 24 h, followed by western blotting. l Similar to k , after pre-transfection of FLAG-MT1H CDS + 3ʹ-UTR for 24 h, changes of MT1H concentrations upon NC-mimic and miR-214 mimic molecules were determined by western blot analysis under Cd treatment at 20 μmol/L for 6 h. m qRT-PCR analysis of MT1DP levels in HepG2 cells transfected with MT1H 3ʹ-UTR and MT1H 3ʹ-UTR + miR-214 ( n = 3). n A working model depicting the interplay of MT1DP with MT1H and RhoC to promote Cd-induced cellular toxicity

    Journal: Cell Discovery

    Article Title: Long non-coding RNA MT1DP shunts the cellular defense to cytotoxicity through crosstalk with MT1H and RhoC in cadmium stress

    doi: 10.1038/s41421-017-0005-y

    Figure Lengend Snippet: MT1DP competes for miR-214 with MT1H. a HepG2 cells were transfected with exotic FLAG-MT1H CDS, FLAG-MT1H CDS + 3ʹ-UTR, and FLAG-MT1H 3ʹ-UTR for 24 h, respectively, and then the MT1DP levels were measured by qRT-PCR ( n = 3). b A schematic illustrating the putative target sites for MT1DP and MT1H in competing for miR-214. c – f Levels of MT1DP and MT1H were determined by qRT-PCR in cells transfected with NC-mimic and miR-214 mimic molecules c , d and NC-inhibitor and miR-214 inhibitor molecules e , f ( n = 3), respectively. g, h Relative luciferase activities in HepG2 cells with expression of pGL3-vehicle, pGL3-MT1DP, and pGL3-MT1DP-mutant constructs g and pGL3-vehicle, pGL3-MT1H 3ʹ-UTR, and pGL3-MT1H 3ʹ-UTR-mutant constructs h upon transfection of NC-mimic and miR-214 mimic molecules, measured through the dual-luciferase assay ( n = 3). i Relative enrichment of miR-214 in the pull-down lysates from cells using MS2-MT1DP and MS2-MT1DP mutant RNAs, respectively, examined by qRT-PCR assay ( n = 3). Fold changes were normalized to U6 RNA levels. j The MT1H levels in HepG2 cells transfected with synthesized molecules of vehicle control, MT1DP, miR-214 and MT1DP + miR-214, detected by qRT-PCR assay ( n = 3). k Western blot analysis of FLAG-MT1H in HepG2 cells. Cells were pre-transfected with FLAG-MT1H CDS + 3ʹ-UTR for 24 h, and were further transfected with synthesized molecules of vehicle control, MT1DP, miR-214 and MT1DP + miRNA-214 for another 24 h, followed by western blotting. l Similar to k , after pre-transfection of FLAG-MT1H CDS + 3ʹ-UTR for 24 h, changes of MT1H concentrations upon NC-mimic and miR-214 mimic molecules were determined by western blot analysis under Cd treatment at 20 μmol/L for 6 h. m qRT-PCR analysis of MT1DP levels in HepG2 cells transfected with MT1H 3ʹ-UTR and MT1H 3ʹ-UTR + miR-214 ( n = 3). n A working model depicting the interplay of MT1DP with MT1H and RhoC to promote Cd-induced cellular toxicity

    Article Snippet: Total RNAs were extracted from cells with Trizol reagent (Invitrogen, USA), and then 2–5 μg RNAs were reverse-transcribed into cDNA with M-MLV reverse transcriptase. qPCR was performed on an iQ5 qRT-PCR instrument (Bio-Rad), as described previously , GAPDH was used as a loading control for normalization.

    Techniques: Transfection, Quantitative RT-PCR, Luciferase, Expressing, Mutagenesis, Construct, Synthesized, Western Blot

    MT1DP induction promotes Cd-induced cell death. a Relative expression levels of MT members in HepG2 cells treated with Cd at 20 μmol/L for 24 h. Inserted panel, western blot analysis of MT1/2 proteins in HepG2 cells treated with Cd for 24 h. b MT1DP levels upon exposure to Cd at various concentrations over time determined by qRT-PCR ( n = 3). c Relative expression levels of MT1DP in HepG2 cells upon treatment with 2 μg/mL cisplatin, 5 μM/mL gefitinib, 50 μg/mL 5-FU, 10 μmol/L arsenic (As), and 20 μmol/L Cd through qRT-PCR assay ( n = 3). d Expression changes of lncRNAs in HepG2 cells exposed to 10 μmol/L Cd for 24 h, as characterized by qRT-PCR analysis ( n = 3). e Relative expression levels of MT1DP in MT1DP low cells. The endogenous MT1DP was stably knocked down in HepG2 cells, as evidenced by qRT-PCR assay ( n = 3), with relative expression levels labeled above the bars for MT1DP-shRNA #1 and MT1DP-shRNA #2 transfectants. Expression changes of MT1DP in cells responded to Cd at 20 μmol/L were also detected by qRT-PCR ( n = 3). f The proportions of PI-positive in scrambled-shRNA control cells and MT1DP-low cells in response to 20 μmol/L Cd for 3, 6, 12 and 24 h, determined by flow cytometry analysis ( n = 3)

    Journal: Cell Discovery

    Article Title: Long non-coding RNA MT1DP shunts the cellular defense to cytotoxicity through crosstalk with MT1H and RhoC in cadmium stress

    doi: 10.1038/s41421-017-0005-y

    Figure Lengend Snippet: MT1DP induction promotes Cd-induced cell death. a Relative expression levels of MT members in HepG2 cells treated with Cd at 20 μmol/L for 24 h. Inserted panel, western blot analysis of MT1/2 proteins in HepG2 cells treated with Cd for 24 h. b MT1DP levels upon exposure to Cd at various concentrations over time determined by qRT-PCR ( n = 3). c Relative expression levels of MT1DP in HepG2 cells upon treatment with 2 μg/mL cisplatin, 5 μM/mL gefitinib, 50 μg/mL 5-FU, 10 μmol/L arsenic (As), and 20 μmol/L Cd through qRT-PCR assay ( n = 3). d Expression changes of lncRNAs in HepG2 cells exposed to 10 μmol/L Cd for 24 h, as characterized by qRT-PCR analysis ( n = 3). e Relative expression levels of MT1DP in MT1DP low cells. The endogenous MT1DP was stably knocked down in HepG2 cells, as evidenced by qRT-PCR assay ( n = 3), with relative expression levels labeled above the bars for MT1DP-shRNA #1 and MT1DP-shRNA #2 transfectants. Expression changes of MT1DP in cells responded to Cd at 20 μmol/L were also detected by qRT-PCR ( n = 3). f The proportions of PI-positive in scrambled-shRNA control cells and MT1DP-low cells in response to 20 μmol/L Cd for 3, 6, 12 and 24 h, determined by flow cytometry analysis ( n = 3)

    Article Snippet: Total RNAs were extracted from cells with Trizol reagent (Invitrogen, USA), and then 2–5 μg RNAs were reverse-transcribed into cDNA with M-MLV reverse transcriptase. qPCR was performed on an iQ5 qRT-PCR instrument (Bio-Rad), as described previously , GAPDH was used as a loading control for normalization.

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Stable Transfection, Labeling, shRNA, Flow Cytometry, Cytometry

    MT1DP interacts with RhoC and increases its protein stability to reinforce cell death upon Cd treatment. a MT1DP partners were surveyed by RNA-pull-down assay and mass spectrometry. b Western blot analysis of RhoC protein concentrations in biotin-MT1DP and biotin-NS pull-down complexes. NS denotes non-sense RNA probes. c RIP assay was performed to determine the binding of RhoC with MT1DP RNA. The enrichment of MT1DP RNA was measured by qRT-PCR assay in normal IgG pull-down complex from vehicle control cells and anti-RhoC Ab pull-down complexes from vehicle control and RhoC overexpression cells, respectively ( n = 3). d The fluorescence of RhoC (in red) and MT1DP (in green) was visualized by confocal microscopy. The red arrows indicate the overlapping (shown in yellow) between RhoC protein fluorescence and MT1DP RNA fluorescence. Nuclei are shown in blue through staining with DAPI. e HepG2 cells were transfected with increasing concentrations of MT1DP overexpression constructs for 24 h, and thereafter the protein contents of RhoC were determined by western blotting. f RhoC protein fluorescence (shown in red) was detected by confocal microscopy in vehicle control and MT1DP overexpression cells. g Western blot analysis of the protein levels of RhoC in vector control and MT1DP overexpression cells in response to 40 μmol/L CHX over the time course. h RhoC protein concentrations in scrambled control and MT1DP low cells treated with 40 μmol/L CHX over time, detected by western blot analysis. HepG2 cells were treated with 20 μmol/L MG132 i and 10 nmol/L BFA j over time, and then the protein levels of RhoC and p53 were determined by western blot. k The protein content of RhoC in scrambled control and MT1DP low cells in response to Cd for 6 h, determined by western blot analysis

    Journal: Cell Discovery

    Article Title: Long non-coding RNA MT1DP shunts the cellular defense to cytotoxicity through crosstalk with MT1H and RhoC in cadmium stress

    doi: 10.1038/s41421-017-0005-y

    Figure Lengend Snippet: MT1DP interacts with RhoC and increases its protein stability to reinforce cell death upon Cd treatment. a MT1DP partners were surveyed by RNA-pull-down assay and mass spectrometry. b Western blot analysis of RhoC protein concentrations in biotin-MT1DP and biotin-NS pull-down complexes. NS denotes non-sense RNA probes. c RIP assay was performed to determine the binding of RhoC with MT1DP RNA. The enrichment of MT1DP RNA was measured by qRT-PCR assay in normal IgG pull-down complex from vehicle control cells and anti-RhoC Ab pull-down complexes from vehicle control and RhoC overexpression cells, respectively ( n = 3). d The fluorescence of RhoC (in red) and MT1DP (in green) was visualized by confocal microscopy. The red arrows indicate the overlapping (shown in yellow) between RhoC protein fluorescence and MT1DP RNA fluorescence. Nuclei are shown in blue through staining with DAPI. e HepG2 cells were transfected with increasing concentrations of MT1DP overexpression constructs for 24 h, and thereafter the protein contents of RhoC were determined by western blotting. f RhoC protein fluorescence (shown in red) was detected by confocal microscopy in vehicle control and MT1DP overexpression cells. g Western blot analysis of the protein levels of RhoC in vector control and MT1DP overexpression cells in response to 40 μmol/L CHX over the time course. h RhoC protein concentrations in scrambled control and MT1DP low cells treated with 40 μmol/L CHX over time, detected by western blot analysis. HepG2 cells were treated with 20 μmol/L MG132 i and 10 nmol/L BFA j over time, and then the protein levels of RhoC and p53 were determined by western blot. k The protein content of RhoC in scrambled control and MT1DP low cells in response to Cd for 6 h, determined by western blot analysis

    Article Snippet: Total RNAs were extracted from cells with Trizol reagent (Invitrogen, USA), and then 2–5 μg RNAs were reverse-transcribed into cDNA with M-MLV reverse transcriptase. qPCR was performed on an iQ5 qRT-PCR instrument (Bio-Rad), as described previously , GAPDH was used as a loading control for normalization.

    Techniques: Pull Down Assay, Mass Spectrometry, Western Blot, Binding Assay, Quantitative RT-PCR, Over Expression, Fluorescence, Confocal Microscopy, Staining, Transfection, Construct, Plasmid Preparation