iodoacetamide Millipore Search Results


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Millipore a mixture of oxidized insulin b chain alkylated by iodoacetamide
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Millipore 2-iodoacetamide (iaa
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Millipore 2 mg ml −1 iodoacetamide
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Millipore 3-(2-iodoacetamido)-proxyl (iap
Structures of the spin labels used in this study and the resulting chemical modification of the cysteine side chain. (A) MTSL, (1-oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) methanethiosulfonate; (B) MSL, 4-maleimido-TEMPO; (C) <t>IAP,</t> <t>3-(2-iodoacetamido)-PROXYL</t> (D) 100G X-band CW- EPR spectrum of IA3 S14C-IAP with labeled transitions indicating the peak-to-peak intensities of the low-field, h(+1), center-field, h(0), and high-field, h(-1), resonances. (E and F) Stack plots of area normalized 100G X-band EPR spectra of IA3 variants S14C and N58C labeled with MTSL, MSL, and IAP in the presence of 0, 15, and 30% TFE.
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Structures of the spin labels used in this study and the resulting chemical modification of the cysteine side chain. (A) MTSL, (1-oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) methanethiosulfonate; (B) MSL, 4-maleimido-TEMPO; (C) IAP, 3-(2-iodoacetamido)-PROXYL (D) 100G X-band CW- EPR spectrum of IA3 S14C-IAP with labeled transitions indicating the peak-to-peak intensities of the low-field, h(+1), center-field, h(0), and high-field, h(-1), resonances. (E and F) Stack plots of area normalized 100G X-band EPR spectra of IA3 variants S14C and N58C labeled with MTSL, MSL, and IAP in the presence of 0, 15, and 30% TFE.

Journal: Protein Science : A Publication of the Protein Society

Article Title: Characterization of the disordered-to-?-helical transition of IA 3 by SDSL-EPR spectroscopy

doi: 10.1002/pro.547

Figure Lengend Snippet: Structures of the spin labels used in this study and the resulting chemical modification of the cysteine side chain. (A) MTSL, (1-oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) methanethiosulfonate; (B) MSL, 4-maleimido-TEMPO; (C) IAP, 3-(2-iodoacetamido)-PROXYL (D) 100G X-band CW- EPR spectrum of IA3 S14C-IAP with labeled transitions indicating the peak-to-peak intensities of the low-field, h(+1), center-field, h(0), and high-field, h(-1), resonances. (E and F) Stack plots of area normalized 100G X-band EPR spectra of IA3 variants S14C and N58C labeled with MTSL, MSL, and IAP in the presence of 0, 15, and 30% TFE.

Article Snippet: 3-(2-iodoacetamido)-PROXYL (IAP) and 4-maleimido-2,2,6,6-tetramethyl-1-piperidinyloxy (4-maleimido-TEMPO, MSL) spin labels were purchased from Sigma Aldrich (St. Louis, MO).

Techniques: Modification, Labeling