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Image Search Results
Journal: Protein Science : A Publication of the Protein Society
Article Title: Characterization of the disordered-to-?-helical transition of IA 3 by SDSL-EPR spectroscopy
doi: 10.1002/pro.547
Figure Lengend Snippet: Structures of the spin labels used in this study and the resulting chemical modification of the cysteine side chain. (A) MTSL, (1-oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) methanethiosulfonate; (B) MSL, 4-maleimido-TEMPO; (C) IAP, 3-(2-iodoacetamido)-PROXYL (D) 100G X-band CW- EPR spectrum of IA3 S14C-IAP with labeled transitions indicating the peak-to-peak intensities of the low-field, h(+1), center-field, h(0), and high-field, h(-1), resonances. (E and F) Stack plots of area normalized 100G X-band EPR spectra of IA3 variants S14C and N58C labeled with MTSL, MSL, and IAP in the presence of 0, 15, and 30% TFE.
Article Snippet: 3-(
Techniques: Modification, Labeling