Journal: PLoS ONE
Article Title: Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand
Figure Lengend Snippet: Identification of indole compounds produced by ectomycorrhizal fungi using high performance liquid chromatography technique. A. Indole compounds standard, B. Uncultivated liquid medium. C. Crude culture extract of Astraeus odoratus . D. Crude culture extract of Gyrodon suthepensis . E. crude culture extract of Phlebopus portentosus . F. Crude culture extract of Pisolithus albus . G. Crude culture extract of Pisolithus orientalis . H. crude culture extract of Scleroderma suthepense . L-Trp = L-tryptophan, TAM = tryptamine, IAM = indole-3-acetamide, ILA = indole-3-lactic acid, IPyA = indole-3-pyruvic acid, IAA = indole-3-acetic acid, IOL = indole-3-ethanol and IAN = indole-3-acetonitrile. The analyses were performed in triplicate.
Article Snippet: Rf values were individually determined for the standards of L-Trp, IAA (Sigma-Aldrich, Germany, Cat No. I-2886,), IAM (Wako Pure Chemicals, Japan, Cat No. 325–42383), indole-3-lactic acid (ILA; Wako Pure Chemicals, Japan, Cat No. 325–45301), IAN (Sigma-Aldrich, Germany, Cat No. 129453), indole-3-ethanol (IOL; Wako Pure Chemicals, Japan, Cat No. 350–15601,), IPyA (Sigma-Aldrich, Germany, Cat No. I-17017) and TAM (Sigma-Aldrich, Germany, Cat No. 193747) under UV light (254 nm).
Techniques: Produced, High Performance Liquid Chromatography