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  • 86
    Addgene inc intron 27
    Intron 27, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intron 27/product/Addgene inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    intron 27 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    88
    Addgene inc guide rna against aavs1 intron 1
    CRISPR/Cas9-mediated generation of syngeneic HEK293 cells expressing a unique copy of MA4 or A4M in the <t>AAVS1</t> safe harbor A. , F. Schematic representation of the donor vector used for insertion of the dTo-MA4 A. or A4M-GFP F. cassette into the AAVS1 locus. dTo, dTomato fluorescent protein; SD, splice donor; SA, splice acceptor; CAG, CMV early enhancer/chicken β actin promoter. Black (5′; junction) and green (3′; junction) arrows depict genomic location of primers used to confirm targeted integration. B. , G. Representative images of dTo-MA4- and A4M-GFP-expressing HEK293 cells after puromycin or G418 selection, respectively. C. , H. Targeted integration analysis of MA4 and A4M into the AAVS1 locus by PCR using primers specific for the 5ʹ (top panels) and 3ʹ (bottom panels) integration junctions. D. , I. <t>RNA</t> expression of MA4 D. and A4M I. in antibiotic-selected cells. E. , J. Homologous recombination confirmed by southern blot analysis after BglII digestion of genomic DNA from puromycin/G418-resistant clones using a MA4 probe E. or an AAVS1 exon2 probe outside the targeting construct J. . A 4Kb band represents a targeted integration of MA4 in PPP1R12C. The 8kb band corresponds to the targeted integration of A4M in PPP1R12C. Untargeted allele gives a 12Kb band J. . L. qPCR of the MA4 targets HOXA9 and PROM1 is comparable between HEK293 cells ectopically expressing MA4 upon CRISPR/Cas9-mediated genome edition (left panel) or lentiviral transduction (right panel) * p
    Guide Rna Against Aavs1 Intron 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guide rna against aavs1 intron 1/product/Addgene inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guide rna against aavs1 intron 1 - by Bioz Stars, 2021-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    CRISPR/Cas9-mediated generation of syngeneic HEK293 cells expressing a unique copy of MA4 or A4M in the AAVS1 safe harbor A. , F. Schematic representation of the donor vector used for insertion of the dTo-MA4 A. or A4M-GFP F. cassette into the AAVS1 locus. dTo, dTomato fluorescent protein; SD, splice donor; SA, splice acceptor; CAG, CMV early enhancer/chicken β actin promoter. Black (5′; junction) and green (3′; junction) arrows depict genomic location of primers used to confirm targeted integration. B. , G. Representative images of dTo-MA4- and A4M-GFP-expressing HEK293 cells after puromycin or G418 selection, respectively. C. , H. Targeted integration analysis of MA4 and A4M into the AAVS1 locus by PCR using primers specific for the 5ʹ (top panels) and 3ʹ (bottom panels) integration junctions. D. , I. RNA expression of MA4 D. and A4M I. in antibiotic-selected cells. E. , J. Homologous recombination confirmed by southern blot analysis after BglII digestion of genomic DNA from puromycin/G418-resistant clones using a MA4 probe E. or an AAVS1 exon2 probe outside the targeting construct J. . A 4Kb band represents a targeted integration of MA4 in PPP1R12C. The 8kb band corresponds to the targeted integration of A4M in PPP1R12C. Untargeted allele gives a 12Kb band J. . L. qPCR of the MA4 targets HOXA9 and PROM1 is comparable between HEK293 cells ectopically expressing MA4 upon CRISPR/Cas9-mediated genome edition (left panel) or lentiviral transduction (right panel) * p

    Journal: Oncotarget

    Article Title: Expression of MLL-AF4 or AF4-MLL fusions does not impact the efficiency of DNA damage repair

    doi: 10.18632/oncotarget.8938

    Figure Lengend Snippet: CRISPR/Cas9-mediated generation of syngeneic HEK293 cells expressing a unique copy of MA4 or A4M in the AAVS1 safe harbor A. , F. Schematic representation of the donor vector used for insertion of the dTo-MA4 A. or A4M-GFP F. cassette into the AAVS1 locus. dTo, dTomato fluorescent protein; SD, splice donor; SA, splice acceptor; CAG, CMV early enhancer/chicken β actin promoter. Black (5′; junction) and green (3′; junction) arrows depict genomic location of primers used to confirm targeted integration. B. , G. Representative images of dTo-MA4- and A4M-GFP-expressing HEK293 cells after puromycin or G418 selection, respectively. C. , H. Targeted integration analysis of MA4 and A4M into the AAVS1 locus by PCR using primers specific for the 5ʹ (top panels) and 3ʹ (bottom panels) integration junctions. D. , I. RNA expression of MA4 D. and A4M I. in antibiotic-selected cells. E. , J. Homologous recombination confirmed by southern blot analysis after BglII digestion of genomic DNA from puromycin/G418-resistant clones using a MA4 probe E. or an AAVS1 exon2 probe outside the targeting construct J. . A 4Kb band represents a targeted integration of MA4 in PPP1R12C. The 8kb band corresponds to the targeted integration of A4M in PPP1R12C. Untargeted allele gives a 12Kb band J. . L. qPCR of the MA4 targets HOXA9 and PROM1 is comparable between HEK293 cells ectopically expressing MA4 upon CRISPR/Cas9-mediated genome edition (left panel) or lentiviral transduction (right panel) * p

    Article Snippet: Guide RNA against AAVS1 intron 1 was constructed using the primers AAVS1 Fw 5ʹ-CACCGGGGCCACTAGGGACAGGAT-3ʹ and AAVS1 Rv 5ʹ-AAACATCCTGTCCCTAGTGGCCCC-3ʹ [ ]. pAAVS1.SA-2A-Puro-pA donor vector was obtained from Addgene (plasmid #22075). pAAVS1.SA-2A-Neo-pA was made by replacing puro gene from pAAVS1.SA-2A-Puro-pA using Xho I and Mfe I restriction sites.

    Techniques: CRISPR, Expressing, Plasmid Preparation, Selection, Polymerase Chain Reaction, RNA Expression, Homologous Recombination, Southern Blot, Clone Assay, Construct, Real-time Polymerase Chain Reaction, Transduction