Journal: Nature Communications
Article Title: The gut microbiota suppresses insulin-mediated fat accumulation via the short-chain fatty acid receptor GPR43
Figure Lengend Snippet: GPR43 suppresses insulin signalling in the adipose tissues but not in muscles or liver. Insulin-stimulated Akt phosphorylation of Ser473 in the WAT ( a , n =3), muscles ( b , n =4) and liver ( c , n =3) of aP2-Gpr43TG mice fed an HFD after 6 h of fasting. ( d – f ) Inhibitory effects of acetate on insulin signalling (1 g kg −1 , i.p.). After pretreatment with acetate for 40 min, a bolus of insulin (0.15 U kg −1 ) with or without acetate (1 g kg −1 ) was administered intraperitoneally. Akt phosphorylation of Ser473 in the WAT ( d , n =3), muscles ( e , n =3) and liver ( f , n =3) of Gpr43 −/− mice after 6 h of fasting. ( g , h ) Effect of acetate on glucose uptake in MEF-derived adipocytes from Gpr43−/− or aP2-Gpr43TG mice ( n =4, respectively). ( i , j ) Effect of acetate on the fatty acid uptake in MEF-derived adipocytes from Gpr43−/− or aP2-Gpr43TG mice ( n =8–15). LPL activity in the WAT ( k , n =4–5) and the muscles ( l , n =3–5) of Gpr43−/− or aP2-Gpr43TG mice ( n =3–4). ( m ) LPL activity of Gpr43−/− mice fed an HFD under GF conditions ( n =4, 6) or aP2-Gpr43TG mice treated with antibiotics ( n =7, 6). All mice were analysed at 15–16 weeks of age. All data are presented as mean±s.e.m. analysis of variance followed by Tukey–Kramer’s post hoc test ( a – j ) and Student’s t -test ( k – m ); * P
Article Snippet: GPR43-mediated suppression of insulin-induced Akt phosphorylation was effectively blocked by the treatment with U73122 (PLC inhibitor) and Go6983 (PKC inhibitor), but not U0126 (MEK inhibitor) ( ).
Techniques: Mouse Assay, Derivative Assay, Activity Assay