insoluble material Search Results


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  • 99
    Millipore guanidine insoluble material
    Guanidine Insoluble Material, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore insoluble material
    Insoluble Material, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beckman Coulter insoluble material
    Insoluble Material, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad insoluble material
    Insoluble Material, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Eppendorf AG insoluble material
    Insoluble Material, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher insoluble material
    Insoluble Material, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Hettich insoluble material
    Insoluble Material, supplied by Hettich, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology insoluble material
    Insoluble Material, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Tianjin Jinteng Experiment insoluble material
    Insoluble Material, supplied by Tianjin Jinteng Experiment, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cytoskeleton Inc insoluble material
    Insoluble Material, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Signet Testing insoluble material
    Insoluble Material, supplied by Signet Testing, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Assay Biotechnology insoluble material
    Insoluble Material, supplied by Assay Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche insoluble material
    Insoluble Material, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam insoluble material
    Insoluble Material, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    VirTis insoluble 3c material
    Partitioning of DNA and histones between soluble and insoluble portions of the <t>3C</t> material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.
    Insoluble 3c Material, supplied by VirTis, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Eppendorf AG residual insoluble material
    Partitioning of DNA and histones between soluble and insoluble portions of the <t>3C</t> material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.
    Residual Insoluble Material, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Beckman Coulter insoluble cellular materials
    Partitioning of DNA and histones between soluble and insoluble portions of the <t>3C</t> material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.
    Insoluble Cellular Materials, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    GE Healthcare separate insoluble material
    Partitioning of DNA and histones between soluble and insoluble portions of the <t>3C</t> material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.
    Separate Insoluble Material, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Eppendorf AG triton insoluble material
    Partitioning of DNA and histones between soluble and insoluble portions of the <t>3C</t> material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.
    Triton Insoluble Material, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore acid insoluble cellular material
    Partitioning of DNA and histones between soluble and insoluble portions of the <t>3C</t> material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.
    Acid Insoluble Cellular Material, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare tca insoluble material
    Partitioning of DNA and histones between soluble and insoluble portions of the <t>3C</t> material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.
    Tca Insoluble Material, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Partitioning of DNA and histones between soluble and insoluble portions of the 3C material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.

    Journal: Nucleic Acids Research

    Article Title: Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub

    doi: 10.1093/nar/gkt067

    Figure Lengend Snippet: Partitioning of DNA and histones between soluble and insoluble portions of the 3C material and size distribution of DNA fragments. ( A ) Relative amounts of DNA in soluble (super) and insoluble (debris) portions of the 3C material, as determined by fluorometric assays (Qubit, Invitrogen). In each experiment, the total amount of DNA in two fractions is set as 100. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the 3C material before and after ligation (agarose gel, ethidium bromide staining). M—DNA size marker (Fermentas, SM0331). ( C and D ) Partitioning of histones between the soluble and the insoluble portions of the 3C material. Proteins present in equal portions of the soluble and the insoluble 3C material were separated by PAAG and visualized by Coomassie staining (C) or by immunoblotting with antibodies against histone H3 (D). The intensity of bands was quantified using ImageJ software. In each experiment, the total amount of histones in two fractions is set as 100. The error bars represent SEM for three independent experiments.

    Article Snippet: In both cases, the preferential ligation of the fragments bearing regulatory elements of the β-globin gene domain was observed in the insoluble 3C material only.

    Techniques: Ligation, Agarose Gel Electrophoresis, Staining, Marker, Software

    Electron microscopic analysis of the insoluble 3C material from liver cells at different steps of the 3C procedure. After formaldehyde cross-linking ( A and A’ ), after isolation of nuclei and extraction with 0.3% SDS followed by 1.8% Triton X-100 ( B and B’ ) and after digestion with HindIII restriction endonuclease followed by extraction with 1.6% SDS ( C and C’ ). Panels below show the enlarged framed region of the above images. Scale bars: 1 µm (A–C) and 250 nm (A’–C’).

    Journal: Nucleic Acids Research

    Article Title: Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub

    doi: 10.1093/nar/gkt067

    Figure Lengend Snippet: Electron microscopic analysis of the insoluble 3C material from liver cells at different steps of the 3C procedure. After formaldehyde cross-linking ( A and A’ ), after isolation of nuclei and extraction with 0.3% SDS followed by 1.8% Triton X-100 ( B and B’ ) and after digestion with HindIII restriction endonuclease followed by extraction with 1.6% SDS ( C and C’ ). Panels below show the enlarged framed region of the above images. Scale bars: 1 µm (A–C) and 250 nm (A’–C’).

    Article Snippet: In both cases, the preferential ligation of the fragments bearing regulatory elements of the β-globin gene domain was observed in the insoluble 3C material only.

    Techniques: Isolation

    Effect of sonication on the DNA partitioning between soluble and insoluble portions of the 3C material and on the frequencies of ligation of the β-globin gene domain fragments. ( A ) Relative amounts of DNA in the soluble and the insoluble portions of the sonicated 3C material. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the sonicated 3C material before and after ligation. ( C ) Results of 3C analysis performed separately on soluble (super) and insoluble (debris) portions of the sonicated 3C material. ( D ) The same as (C) after normalization of the ligation frequencies to the amount of DNA in the samples. ( E ) Results of standard 3C analysis performed on the sonicated 3C material without fractionating into the soluble and the insoluble portions. The sonication time is indicated in seconds. The error bars represent SEM for two independent experiments. Other designations are as in Figure 1 and 2 .

    Journal: Nucleic Acids Research

    Article Title: Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub

    doi: 10.1093/nar/gkt067

    Figure Lengend Snippet: Effect of sonication on the DNA partitioning between soluble and insoluble portions of the 3C material and on the frequencies of ligation of the β-globin gene domain fragments. ( A ) Relative amounts of DNA in the soluble and the insoluble portions of the sonicated 3C material. ( B ) Electrophoretic separation of DNA from soluble and insoluble portions of the sonicated 3C material before and after ligation. ( C ) Results of 3C analysis performed separately on soluble (super) and insoluble (debris) portions of the sonicated 3C material. ( D ) The same as (C) after normalization of the ligation frequencies to the amount of DNA in the samples. ( E ) Results of standard 3C analysis performed on the sonicated 3C material without fractionating into the soluble and the insoluble portions. The sonication time is indicated in seconds. The error bars represent SEM for two independent experiments. Other designations are as in Figure 1 and 2 .

    Article Snippet: In both cases, the preferential ligation of the fragments bearing regulatory elements of the β-globin gene domain was observed in the insoluble 3C material only.

    Techniques: Sonication, Ligation

    Frequencies of ligation of the fragment harboring the Hbb-b1 promoter with several selected fragments of the β-globin gene domain in soluble and insoluble portions of the 3C material. ( A ) Results of standard 3C analysis performed without fractionating the 3C material. ( B ) Results of 3C analysis performed separately on soluble (super) and insoluble (debris) fractions. ( C ) The same as (B) after normalization of the ligation frequencies to the amount of DNA in the samples. ( D ) The same as (C), soluble fraction only. On the top of each graph, a map of the domain is shown, with β-globin genes, olfactory receptor genes and DNase I hypersensitive sites shown by red arrows, blue arrows and black vertical lines, respectively. Plotted on the horizontal axis are the fragment positions. The scale is in kilobases, and according to GenBank entry NT_039433, the ‘0’ point corresponds to the start of the Hbb-y gene. The black rectangle in the background of each graph shows the anchor fragment, and the gray rectangles indicate test fragments. Plotted on the vertical axis are the ligation frequencies; the highest ligation frequency observed is set to 100 [the frequency of ligation between the anchor fragment and the upstream restriction fragment in the total 3C material from fetal liver cells (A) or in the insoluble portion of the 3C material from fetal liver cells (B and C) or the soluble portion of the 3C material from fetal brain cells (D)]. Red and blue lines show the results for liver and brain cells, respectively; solid lines show the results for the total 3C material (A) or the insoluble portion of the 3C material (B and C); dotted lines show the results for the soluble portion of the 3C material. Ligation frequencies of HindIII and MboI fragments are presented on the left and the right graphs, respectively. The error bars represent SEM for three independent experiments.

    Journal: Nucleic Acids Research

    Article Title: Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub

    doi: 10.1093/nar/gkt067

    Figure Lengend Snippet: Frequencies of ligation of the fragment harboring the Hbb-b1 promoter with several selected fragments of the β-globin gene domain in soluble and insoluble portions of the 3C material. ( A ) Results of standard 3C analysis performed without fractionating the 3C material. ( B ) Results of 3C analysis performed separately on soluble (super) and insoluble (debris) fractions. ( C ) The same as (B) after normalization of the ligation frequencies to the amount of DNA in the samples. ( D ) The same as (C), soluble fraction only. On the top of each graph, a map of the domain is shown, with β-globin genes, olfactory receptor genes and DNase I hypersensitive sites shown by red arrows, blue arrows and black vertical lines, respectively. Plotted on the horizontal axis are the fragment positions. The scale is in kilobases, and according to GenBank entry NT_039433, the ‘0’ point corresponds to the start of the Hbb-y gene. The black rectangle in the background of each graph shows the anchor fragment, and the gray rectangles indicate test fragments. Plotted on the vertical axis are the ligation frequencies; the highest ligation frequency observed is set to 100 [the frequency of ligation between the anchor fragment and the upstream restriction fragment in the total 3C material from fetal liver cells (A) or in the insoluble portion of the 3C material from fetal liver cells (B and C) or the soluble portion of the 3C material from fetal brain cells (D)]. Red and blue lines show the results for liver and brain cells, respectively; solid lines show the results for the total 3C material (A) or the insoluble portion of the 3C material (B and C); dotted lines show the results for the soluble portion of the 3C material. Ligation frequencies of HindIII and MboI fragments are presented on the left and the right graphs, respectively. The error bars represent SEM for three independent experiments.

    Article Snippet: In both cases, the preferential ligation of the fragments bearing regulatory elements of the β-globin gene domain was observed in the insoluble 3C material only.

    Techniques: Ligation