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  • 99
    ATCC hela cells
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hela cells/product/ATCC
    Average 99 stars, based on 24553 article reviews
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    hela cells - by Bioz Stars, 2020-08
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    99
    Millipore monoclonal anti alpha tubulin antibody
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Monoclonal Anti Alpha Tubulin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 19851 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti alpha tubulin antibody/product/Millipore
    Average 99 stars, based on 19851 article reviews
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    99
    TaKaRa e coli stellar cells
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    E Coli Stellar Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli stellar cells/product/TaKaRa
    Average 99 stars, based on 86 article reviews
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    e coli stellar cells - by Bioz Stars, 2020-08
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    94
    Thermo Fisher pdonr207
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Pdonr207, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdonr207/product/Thermo Fisher
    Average 94 stars, based on 3493 article reviews
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    pdonr207 - by Bioz Stars, 2020-08
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    99
    Millipore monoclonal anti beta actin antibody
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Monoclonal Anti Beta Actin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 33074 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti beta actin antibody/product/Millipore
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    99
    Millipore anti flag magnetic beads
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Anti Flag Magnetic Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore monoclonal anti flag m2 antibody
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Monoclonal Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 37942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti flag m2 antibody/product/Millipore
    Average 99 stars, based on 37942 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti flag m2 antibody - by Bioz Stars, 2020-08
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    Image Search Results


    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live HeLa at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were transfected to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.

    Journal: Biophysical Journal

    Article Title: A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection

    doi: 10.1016/j.bpj.2018.03.016

    Figure Lengend Snippet: Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live HeLa at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were transfected to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.

    Article Snippet: We transfected HeLa cells with sfCh1-10, encoding for the nonfluorescent strands 1–10 of super-folder Cherry (see for sequence information), followed by infection with ΔinlC pInlC-inlC-sfCh11 for 24 h. As expected for a 24 h infection, all HeLa cells in the field of view presented in a were infected with Listeria ( a , inset and arrows in DIC channel).

    Techniques: Infection, Sequencing, Transfection, Marker, Microscopy

    Tagged InlC is produced and secreted into the HeLa host cell in the context of Listeria ) and an Alexa-Fluor-488-labeled secondary antibody. Representative Listeria cells are indicated in the insets in the DIC channel, and actin tails are indicated by arrows in the actin channel. Images are presented at the same intensity levels for each channel. Scale bars, 20 μ m (wt infection: n = 36 infected cells total; ΔinlC : n = 29 infected cells total; ΔinlC Phyper-inlC-GFP11 : n = 63 infected cells total). To see this figure in color, go online.

    Journal: Biophysical Journal

    Article Title: A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection

    doi: 10.1016/j.bpj.2018.03.016

    Figure Lengend Snippet: Tagged InlC is produced and secreted into the HeLa host cell in the context of Listeria ) and an Alexa-Fluor-488-labeled secondary antibody. Representative Listeria cells are indicated in the insets in the DIC channel, and actin tails are indicated by arrows in the actin channel. Images are presented at the same intensity levels for each channel. Scale bars, 20 μ m (wt infection: n = 36 infected cells total; ΔinlC : n = 29 infected cells total; ΔinlC Phyper-inlC-GFP11 : n = 63 infected cells total). To see this figure in color, go online.

    Article Snippet: We transfected HeLa cells with sfCh1-10, encoding for the nonfluorescent strands 1–10 of super-folder Cherry (see for sequence information), followed by infection with ΔinlC pInlC-inlC-sfCh11 for 24 h. As expected for a 24 h infection, all HeLa cells in the field of view presented in a were infected with Listeria ( a , inset and arrows in DIC channel).

    Techniques: Produced, Labeling, Infection

    High throughout quantification of GFP complementation during Listeria infection. ( a ) HeLa cells were infected with ΔinlC Phyper-inlC-GFP11 and ΔinlC pInlC-inlC-GFP11 side by side, and infections were visualized over time in parallel in the same experimental setup. A representative infection ( ΔinlC pInlC-inlC-GFP11 ) at 3 h postinfection ( left panel ) vs. 6.5 h postinfection ( right panel ) is shown. The inset indicates examples of Listeria cells in the DIC channel. Scale bars, 20 μ m. Images in the GFP channel are presented at the same intensity levels. ( b ) Quantification of GFP fluorescence intensity for the time course shown in ( a ) is given. The arrows indicate 3 and 6.5 h postinfection. ( c ) The mean GFP fluorescence signal for all cells in the experiment was determined at 3 h postinfection vs. 6.5 h postinfection (one experiment; n = 128 cells for ΔinlC Phyper-inlC-GFP11 and n = 75 cells for ΔinlC pInlC-inlC-GFP11 ). Statistical significance was determined using a Tukey honest significant difference ANOVA test. To see this figure in color, go online.

    Journal: Biophysical Journal

    Article Title: A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection

    doi: 10.1016/j.bpj.2018.03.016

    Figure Lengend Snippet: High throughout quantification of GFP complementation during Listeria infection. ( a ) HeLa cells were infected with ΔinlC Phyper-inlC-GFP11 and ΔinlC pInlC-inlC-GFP11 side by side, and infections were visualized over time in parallel in the same experimental setup. A representative infection ( ΔinlC pInlC-inlC-GFP11 ) at 3 h postinfection ( left panel ) vs. 6.5 h postinfection ( right panel ) is shown. The inset indicates examples of Listeria cells in the DIC channel. Scale bars, 20 μ m. Images in the GFP channel are presented at the same intensity levels. ( b ) Quantification of GFP fluorescence intensity for the time course shown in ( a ) is given. The arrows indicate 3 and 6.5 h postinfection. ( c ) The mean GFP fluorescence signal for all cells in the experiment was determined at 3 h postinfection vs. 6.5 h postinfection (one experiment; n = 128 cells for ΔinlC Phyper-inlC-GFP11 and n = 75 cells for ΔinlC pInlC-inlC-GFP11 ). Statistical significance was determined using a Tukey honest significant difference ANOVA test. To see this figure in color, go online.

    Article Snippet: We transfected HeLa cells with sfCh1-10, encoding for the nonfluorescent strands 1–10 of super-folder Cherry (see for sequence information), followed by infection with ΔinlC pInlC-inlC-sfCh11 for 24 h. As expected for a 24 h infection, all HeLa cells in the field of view presented in a were infected with Listeria ( a , inset and arrows in DIC channel).

    Techniques: Infection, Fluorescence