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    ATCC hela cells
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hela cells/product/ATCC
    Average 99 stars, based on 24553 article reviews
    Price from $9.99 to $1999.99
    hela cells - by Bioz Stars, 2020-11
    99/100 stars
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    99
    Thermo Fisher oligonucleotides harboring gateway recombination att b1 2
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Oligonucleotides Harboring Gateway Recombination Att B1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligonucleotides harboring gateway recombination att b1 2/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    oligonucleotides harboring gateway recombination att b1 2 - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    98
    Thermo Fisher pdonr207
    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live <t>HeLa</t> at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were <t>transfected</t> to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.
    Pdonr207, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 3493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdonr207/product/Thermo Fisher
    Average 98 stars, based on 3493 article reviews
    Price from $9.99 to $1999.99
    pdonr207 - by Bioz Stars, 2020-11
    98/100 stars
      Buy from Supplier

    Image Search Results


    Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live HeLa at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were transfected to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.

    Journal: Biophysical Journal

    Article Title: A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection

    doi: 10.1016/j.bpj.2018.03.016

    Figure Lengend Snippet: Split-GFP tagging of InlC during infection of epithelial cells. ( a for sequence information) and a nuclear TagBFP transfection marker are shown. Shown are cells infected with ΔinlC Phyper-inlC-GFP11 for tag sequence information) at 18 h postinfection ( top panel , two experiments, 15 cells) and an uninfected control ( bottom panel ). Insets in DIC channel are examples of Listeria . Arrows in the InlC-GFP channel are examples of InlC cell membrane puncta. ( b ) Microscopy images of live HeLa at different time points postinfection with ΔinlC Phyper-inlC-GFP11 are shown. Listeria entered the cell shown here at 6 h postinfection (see arrows in DIC inset) and accumulate within the host cell throughout the time course. Cells were transfected to produce GFP1-10 and a nuclear transfection marker. Scale bars, 20 μ m. To see this figure in color, go online.

    Article Snippet: We transfected HeLa cells with sfCh1-10, encoding for the nonfluorescent strands 1–10 of super-folder Cherry (see for sequence information), followed by infection with ΔinlC pInlC-inlC-sfCh11 for 24 h. As expected for a 24 h infection, all HeLa cells in the field of view presented in a were infected with Listeria ( a , inset and arrows in DIC channel).

    Techniques: Infection, Sequencing, Transfection, Marker, Microscopy

    Tagged InlC is produced and secreted into the HeLa host cell in the context of Listeria ) and an Alexa-Fluor-488-labeled secondary antibody. Representative Listeria cells are indicated in the insets in the DIC channel, and actin tails are indicated by arrows in the actin channel. Images are presented at the same intensity levels for each channel. Scale bars, 20 μ m (wt infection: n = 36 infected cells total; ΔinlC : n = 29 infected cells total; ΔinlC Phyper-inlC-GFP11 : n = 63 infected cells total). To see this figure in color, go online.

    Journal: Biophysical Journal

    Article Title: A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection

    doi: 10.1016/j.bpj.2018.03.016

    Figure Lengend Snippet: Tagged InlC is produced and secreted into the HeLa host cell in the context of Listeria ) and an Alexa-Fluor-488-labeled secondary antibody. Representative Listeria cells are indicated in the insets in the DIC channel, and actin tails are indicated by arrows in the actin channel. Images are presented at the same intensity levels for each channel. Scale bars, 20 μ m (wt infection: n = 36 infected cells total; ΔinlC : n = 29 infected cells total; ΔinlC Phyper-inlC-GFP11 : n = 63 infected cells total). To see this figure in color, go online.

    Article Snippet: We transfected HeLa cells with sfCh1-10, encoding for the nonfluorescent strands 1–10 of super-folder Cherry (see for sequence information), followed by infection with ΔinlC pInlC-inlC-sfCh11 for 24 h. As expected for a 24 h infection, all HeLa cells in the field of view presented in a were infected with Listeria ( a , inset and arrows in DIC channel).

    Techniques: Produced, Labeling, Infection

    High throughout quantification of GFP complementation during Listeria infection. ( a ) HeLa cells were infected with ΔinlC Phyper-inlC-GFP11 and ΔinlC pInlC-inlC-GFP11 side by side, and infections were visualized over time in parallel in the same experimental setup. A representative infection ( ΔinlC pInlC-inlC-GFP11 ) at 3 h postinfection ( left panel ) vs. 6.5 h postinfection ( right panel ) is shown. The inset indicates examples of Listeria cells in the DIC channel. Scale bars, 20 μ m. Images in the GFP channel are presented at the same intensity levels. ( b ) Quantification of GFP fluorescence intensity for the time course shown in ( a ) is given. The arrows indicate 3 and 6.5 h postinfection. ( c ) The mean GFP fluorescence signal for all cells in the experiment was determined at 3 h postinfection vs. 6.5 h postinfection (one experiment; n = 128 cells for ΔinlC Phyper-inlC-GFP11 and n = 75 cells for ΔinlC pInlC-inlC-GFP11 ). Statistical significance was determined using a Tukey honest significant difference ANOVA test. To see this figure in color, go online.

    Journal: Biophysical Journal

    Article Title: A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection

    doi: 10.1016/j.bpj.2018.03.016

    Figure Lengend Snippet: High throughout quantification of GFP complementation during Listeria infection. ( a ) HeLa cells were infected with ΔinlC Phyper-inlC-GFP11 and ΔinlC pInlC-inlC-GFP11 side by side, and infections were visualized over time in parallel in the same experimental setup. A representative infection ( ΔinlC pInlC-inlC-GFP11 ) at 3 h postinfection ( left panel ) vs. 6.5 h postinfection ( right panel ) is shown. The inset indicates examples of Listeria cells in the DIC channel. Scale bars, 20 μ m. Images in the GFP channel are presented at the same intensity levels. ( b ) Quantification of GFP fluorescence intensity for the time course shown in ( a ) is given. The arrows indicate 3 and 6.5 h postinfection. ( c ) The mean GFP fluorescence signal for all cells in the experiment was determined at 3 h postinfection vs. 6.5 h postinfection (one experiment; n = 128 cells for ΔinlC Phyper-inlC-GFP11 and n = 75 cells for ΔinlC pInlC-inlC-GFP11 ). Statistical significance was determined using a Tukey honest significant difference ANOVA test. To see this figure in color, go online.

    Article Snippet: We transfected HeLa cells with sfCh1-10, encoding for the nonfluorescent strands 1–10 of super-folder Cherry (see for sequence information), followed by infection with ΔinlC pInlC-inlC-sfCh11 for 24 h. As expected for a 24 h infection, all HeLa cells in the field of view presented in a were infected with Listeria ( a , inset and arrows in DIC channel).

    Techniques: Infection, Fluorescence