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  • 99
    Thermo Fisher fluo 4 nw calcium assay kit
    Effects of palmitic and linoleic acids on apoptosis, caspase-3 activity and calcium flux in H4IIE liver cells . Rat liver cells were treated with thapsigargin (TG), palmitic acid (PA, 250 μM), linoleic acid (LA, 250 μM) alone or the combination of PA (250 μM) and LA (125 or 250 μM) for 16 hours. The cells were treated with phosphate buffered saline (PBS) as Control group. (A) Apoptotic index was measured by determining the cytoplasmic histone-associated DNA fragments with an ELISA assay. (B) The caspase-3 level was detected according to a colorimetric assay that measures the extent of cleavage of a caspase-specific peptide derivatized with p-nitroalanine. (C) The calcium level was determined with the <t>Fluo-4</t> NW calcium assay kit. a: P
    Fluo 4 Nw Calcium Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fluo 4 nw calcium assay kit - by Bioz Stars, 2020-04
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    99
    Millipore mtt assay in vitro toxicology assay kit mtt based m 5655 sigma st louis mo
    Effects of palmitic and linoleic acids on apoptosis, caspase-3 activity and calcium flux in H4IIE liver cells . Rat liver cells were treated with thapsigargin (TG), palmitic acid (PA, 250 μM), linoleic acid (LA, 250 μM) alone or the combination of PA (250 μM) and LA (125 or 250 μM) for 16 hours. The cells were treated with phosphate buffered saline (PBS) as Control group. (A) Apoptotic index was measured by determining the cytoplasmic histone-associated DNA fragments with an ELISA assay. (B) The caspase-3 level was detected according to a colorimetric assay that measures the extent of cleavage of a caspase-specific peptide derivatized with p-nitroalanine. (C) The calcium level was determined with the <t>Fluo-4</t> NW calcium assay kit. a: P
    Mtt Assay In Vitro Toxicology Assay Kit Mtt Based M 5655 Sigma St Louis Mo, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtt assay in vitro toxicology assay kit mtt based m 5655 sigma st louis mo/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    99
    Millipore gssg gsh quantification kit
    Effects of palmitic and linoleic acids on apoptosis, caspase-3 activity and calcium flux in H4IIE liver cells . Rat liver cells were treated with thapsigargin (TG), palmitic acid (PA, 250 μM), linoleic acid (LA, 250 μM) alone or the combination of PA (250 μM) and LA (125 or 250 μM) for 16 hours. The cells were treated with phosphate buffered saline (PBS) as Control group. (A) Apoptotic index was measured by determining the cytoplasmic histone-associated DNA fragments with an ELISA assay. (B) The caspase-3 level was detected according to a colorimetric assay that measures the extent of cleavage of a caspase-specific peptide derivatized with p-nitroalanine. (C) The calcium level was determined with the <t>Fluo-4</t> NW calcium assay kit. a: P
    Gssg Gsh Quantification Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gssg gsh quantification kit/product/Millipore
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    Price from $9.99 to $1999.99
    gssg gsh quantification kit - by Bioz Stars, 2020-04
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    Millipore cell proliferation elisa bromodeoxyuridine brdu assay
    Effects of palmitic and linoleic acids on apoptosis, caspase-3 activity and calcium flux in H4IIE liver cells . Rat liver cells were treated with thapsigargin (TG), palmitic acid (PA, 250 μM), linoleic acid (LA, 250 μM) alone or the combination of PA (250 μM) and LA (125 or 250 μM) for 16 hours. The cells were treated with phosphate buffered saline (PBS) as Control group. (A) Apoptotic index was measured by determining the cytoplasmic histone-associated DNA fragments with an ELISA assay. (B) The caspase-3 level was detected according to a colorimetric assay that measures the extent of cleavage of a caspase-specific peptide derivatized with p-nitroalanine. (C) The calcium level was determined with the <t>Fluo-4</t> NW calcium assay kit. a: P
    Cell Proliferation Elisa Bromodeoxyuridine Brdu Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell proliferation elisa bromodeoxyuridine brdu assay/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cell proliferation elisa bromodeoxyuridine brdu assay - by Bioz Stars, 2020-04
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    Effects of palmitic and linoleic acids on apoptosis, caspase-3 activity and calcium flux in H4IIE liver cells . Rat liver cells were treated with thapsigargin (TG), palmitic acid (PA, 250 μM), linoleic acid (LA, 250 μM) alone or the combination of PA (250 μM) and LA (125 or 250 μM) for 16 hours. The cells were treated with phosphate buffered saline (PBS) as Control group. (A) Apoptotic index was measured by determining the cytoplasmic histone-associated DNA fragments with an ELISA assay. (B) The caspase-3 level was detected according to a colorimetric assay that measures the extent of cleavage of a caspase-specific peptide derivatized with p-nitroalanine. (C) The calcium level was determined with the Fluo-4 NW calcium assay kit. a: P

    Journal: Lipids in Health and Disease

    Article Title: Palmitic and linoleic acids induce ER stress and apoptosis in hepatoma cells

    doi: 10.1186/1476-511X-11-1

    Figure Lengend Snippet: Effects of palmitic and linoleic acids on apoptosis, caspase-3 activity and calcium flux in H4IIE liver cells . Rat liver cells were treated with thapsigargin (TG), palmitic acid (PA, 250 μM), linoleic acid (LA, 250 μM) alone or the combination of PA (250 μM) and LA (125 or 250 μM) for 16 hours. The cells were treated with phosphate buffered saline (PBS) as Control group. (A) Apoptotic index was measured by determining the cytoplasmic histone-associated DNA fragments with an ELISA assay. (B) The caspase-3 level was detected according to a colorimetric assay that measures the extent of cleavage of a caspase-specific peptide derivatized with p-nitroalanine. (C) The calcium level was determined with the Fluo-4 NW calcium assay kit. a: P

    Article Snippet: Measurement of calcium flux Changes in calcium flux were determined with the Fluo-4 NW calcium assay kit (Molecular Probes, Eugene, OR).

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, Calcium Assay