in-fusion reaction mix Search Results


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  • 99
    New England Biolabs hifi dna assembly master mix
    Hifi Dna Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fusion high fidelity pcr master mix 2×
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    Becton Dickinson in fusion reaction mix
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    Agilent technologies standard herculase ii fusion dna polymerase reaction mix
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    TaKaRa down mix kit
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    Thermo Fisher accuprime pfx fusion mix
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    Promega tag green master mix
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    Agilent technologies herculase ii fusion pcr mix
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    TaKaRa in fusion cloning mixture
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    Agilent technologies pfuultra ii fusion hs dna polymerase master mix
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    TaKaRa in fusion hd ecodry enzyme mix
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    Agilent technologies pcr master mix
    Pcr Master Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs phusion high fidelity pcr master mix with hf buffer
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    Roche rna gst cpeb fusion protein incubation mixture
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Rna Gst Cpeb Fusion Protein Incubation Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa in fusion hd cloning kit
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp mll ell fusion hs03024474 ft
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Gene Exp Mll Ell Fusion Hs03024474 Ft, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp mll mllt3 fusion hs03296416 ft
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Gene Exp Mll Mllt3 Fusion Hs03296416 Ft, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp rbm15 mkl1 fusion hs03024505 ft
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Gene Exp Rbm15 Mkl1 Fusion Hs03024505 Ft, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc gsk 3 fusion protein
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Gsk 3 Fusion Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher herculase ii reaction buffer
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Herculase Ii Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies herculase ii fusion polymerase
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Herculase Ii Fusion Polymerase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies pfuultra fusion hotstart dna polymerase
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Pfuultra Fusion Hotstart Dna Polymerase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ha tagged fusion proteins
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Ha Tagged Fusion Proteins, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher electromax dh10b cells
    <t>CPEB</t> binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a <t>GST-CPEB</t> fusion protein in COS7 cells and combined with in vitro transcribed <t>RNA</t> encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.
    Electromax Dh10b Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 968 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CPEB binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a GST-CPEB fusion protein in COS7 cells and combined with in vitro transcribed RNA encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.

    Journal: The Journal of Neuroscience

    Article Title: Rapid, Activity-Induced Increase in Tissue Plasminogen Activator Is Mediated by Metabotropic Glutamate Receptor-Dependent mRNA Translation

    doi: 10.1523/JNEUROSCI.2457-04.2004

    Figure Lengend Snippet: CPEB binds tPA mRNA through an interaction with the 3′-UTR. A , Native CPEB binds to endogenous tPA mRNA. CPEB was immunoprecipitated (IP) from rat brain homogenate using either an anti-CPEB antibody (α-CPEB) or a nonspecific IgG (CON) and Western blotted to determine the specificity of the IP (left panel). RT-PCR was then performed after the IPs to determine what mRNAs were bound by CPEB. The CPE-containing mRNAs encoding α-CaMKII and tPA were both found to associate with CPEB, whereas the non-CPE-containing mRNA encoding GFAP was not (right panel). B, Full-length human CPEB was expressed as a GST-CPEB fusion protein in COS7 cells and combined with in vitro transcribed RNA encoding the entire 3′-UTR of rat tPA mRNA. The top panel is the RT-PCR product of the input RNA indicating equivalent loading. The bottom panel shows the RT-PCR product if GST alone (CON) or GST-CPEB was used to pull down tPA 3′-UTR.

    Article Snippet: To minimize RNA degradation, all the reagents were prepared in RNase-free buffer, and RNase inhibitor was added to RNA-GST CPEB fusion protein incubation mixture at a final concentration of 200 U/ml (Roche, Mannheim, Germany).

    Techniques: Immunoprecipitation, Western Blot, Reverse Transcription Polymerase Chain Reaction, In Vitro