in‐vitro blood tests Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    ATCC candida albicans isolates
    Candida Albicans Isolates, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/candida albicans isolates/product/ATCC
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    candida albicans isolates - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher indiko and konelab cholesterol kit
    Indiko And Konelab Cholesterol Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/indiko and konelab cholesterol kit/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    indiko and konelab cholesterol kit - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    86
    Cellestis quantiferon tb gold blood test
    Quantiferon Tb Gold Blood Test, supplied by Cellestis, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantiferon tb gold blood test/product/Cellestis
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    quantiferon tb gold blood test - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    88
    Roche enzymatic in vitro tests
    Enzymatic In Vitro Tests, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic in vitro tests/product/Roche
    Average 88 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    enzymatic in vitro tests - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    85
    Roche enzymatic in vitro test
    Enzymatic In Vitro Test, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic in vitro test/product/Roche
    Average 85 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    enzymatic in vitro test - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    85
    Nova Biomedical amperometric in vitro test
    Amperometric In Vitro Test, supplied by Nova Biomedical, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amperometric in vitro test/product/Nova Biomedical
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    amperometric in vitro test - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    85
    Roche in vitro nucleic acid amplification test
    In Vitro Nucleic Acid Amplification Test, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro nucleic acid amplification test/product/Roche
    Average 85 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    in vitro nucleic acid amplification test - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    99
    Millipore tox7 kit
    Tox7 Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tox7 kit/product/Millipore
    Average 99 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    tox7 kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    98
    Millipore in vitro plasma stability assay anti her2 dvd adc
    Assembly of <t>DVD-ADCs</t> and in vitro efficacy. a Structure of β-lactam MMAF. b <t>anti-HER2</t> <t>DVD-ADC</t> was assembled by incubating the anti-HER2 DVD with four equivalents (eq) of β-lactam MMAF at room temperature (rt) for 4 h. Drug attachment (red star) occurs at the reactive Lys of h38C2 (yellow circle), located in the heavy chain variable domain of anti-HER2 DVD, to form a stable amide bond. c ESI-MS was performed with unconjugated anti-HER2 DVD and conjugated anti-HER2 DVD-ADC after reduction (10 mM DTT) and deglycosylation (PNGase F). No conjugation was detected on the light chain and the increase in mass of the heavy chain (~1160 Da) corresponds to the addition of exactly one eq of β-lactam MMAF. No unconjugated or higher drug loaded species were detected. d Cytotoxicity of anti-HER2 DVD-ADC (red) following incubation with HER2+ BC cell line SK-BR-3 and HER2− BC cell line MDA-MB-468 for 72 h at 37 °C (mean ± SD of triplicates). Unconjugated anti-HER2 DVD (blue) was significantly less toxic to SK-BR-3 cells when compared to anti-HER2 DVD-ADC ( p
    In Vitro Plasma Stability Assay Anti Her2 Dvd Adc, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro plasma stability assay anti her2 dvd adc/product/Millipore
    Average 98 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    in vitro plasma stability assay anti her2 dvd adc - by Bioz Stars, 2020-05
    98/100 stars
      Buy from Supplier

    95
    Bio-Rad biorad ih 1000
    Assembly of <t>DVD-ADCs</t> and in vitro efficacy. a Structure of β-lactam MMAF. b <t>anti-HER2</t> <t>DVD-ADC</t> was assembled by incubating the anti-HER2 DVD with four equivalents (eq) of β-lactam MMAF at room temperature (rt) for 4 h. Drug attachment (red star) occurs at the reactive Lys of h38C2 (yellow circle), located in the heavy chain variable domain of anti-HER2 DVD, to form a stable amide bond. c ESI-MS was performed with unconjugated anti-HER2 DVD and conjugated anti-HER2 DVD-ADC after reduction (10 mM DTT) and deglycosylation (PNGase F). No conjugation was detected on the light chain and the increase in mass of the heavy chain (~1160 Da) corresponds to the addition of exactly one eq of β-lactam MMAF. No unconjugated or higher drug loaded species were detected. d Cytotoxicity of anti-HER2 DVD-ADC (red) following incubation with HER2+ BC cell line SK-BR-3 and HER2− BC cell line MDA-MB-468 for 72 h at 37 °C (mean ± SD of triplicates). Unconjugated anti-HER2 DVD (blue) was significantly less toxic to SK-BR-3 cells when compared to anti-HER2 DVD-ADC ( p
    Biorad Ih 1000, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biorad ih 1000/product/Bio-Rad
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biorad ih 1000 - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    99
    Bio-Rad calcium free phosphate buffered saline pbs
    Assembly of <t>DVD-ADCs</t> and in vitro efficacy. a Structure of β-lactam MMAF. b <t>anti-HER2</t> <t>DVD-ADC</t> was assembled by incubating the anti-HER2 DVD with four equivalents (eq) of β-lactam MMAF at room temperature (rt) for 4 h. Drug attachment (red star) occurs at the reactive Lys of h38C2 (yellow circle), located in the heavy chain variable domain of anti-HER2 DVD, to form a stable amide bond. c ESI-MS was performed with unconjugated anti-HER2 DVD and conjugated anti-HER2 DVD-ADC after reduction (10 mM DTT) and deglycosylation (PNGase F). No conjugation was detected on the light chain and the increase in mass of the heavy chain (~1160 Da) corresponds to the addition of exactly one eq of β-lactam MMAF. No unconjugated or higher drug loaded species were detected. d Cytotoxicity of anti-HER2 DVD-ADC (red) following incubation with HER2+ BC cell line SK-BR-3 and HER2− BC cell line MDA-MB-468 for 72 h at 37 °C (mean ± SD of triplicates). Unconjugated anti-HER2 DVD (blue) was significantly less toxic to SK-BR-3 cells when compared to anti-HER2 DVD-ADC ( p
    Calcium Free Phosphate Buffered Saline Pbs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcium free phosphate buffered saline pbs/product/Bio-Rad
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    calcium free phosphate buffered saline pbs - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    90
    BioFire Defense filmarray blood culture identification bcid panel
    Performance in identification of polymicrobial blood cultures with the <t>FilmArray</t> <t>BCID</t> panel
    Filmarray Blood Culture Identification Bcid Panel, supplied by BioFire Defense, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filmarray blood culture identification bcid panel/product/BioFire Defense
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    filmarray blood culture identification bcid panel - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    86
    Luminex verigene gram negative blood culture bc gn assay
    Performance in identification of polymicrobial blood cultures with the <t>FilmArray</t> <t>BCID</t> panel
    Verigene Gram Negative Blood Culture Bc Gn Assay, supplied by Luminex, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/verigene gram negative blood culture bc gn assay/product/Luminex
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    verigene gram negative blood culture bc gn assay - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher opti mem reduced serum medium
    Performance in identification of polymicrobial blood cultures with the <t>FilmArray</t> <t>BCID</t> panel
    Opti Mem Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opti mem reduced serum medium/product/Thermo Fisher
    Average 99 stars, based on 3587 article reviews
    Price from $9.99 to $1999.99
    opti mem reduced serum medium - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Abcam antibody occludin
    In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein <t>occludin.</t> (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p
    Antibody Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody occludin/product/Abcam
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    antibody occludin - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    90
    Luminex gram negative blood culture bc gn panels
    In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein <t>occludin.</t> (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p
    Gram Negative Blood Culture Bc Gn Panels, supplied by Luminex, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gram negative blood culture bc gn panels/product/Luminex
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    gram negative blood culture bc gn panels - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    99
    ATCC streptococcus pneumoniae
    In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein <t>occludin.</t> (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p
    Streptococcus Pneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptococcus pneumoniae/product/ATCC
    Average 99 stars, based on 415 article reviews
    Price from $9.99 to $1999.99
    streptococcus pneumoniae - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Millipore p berghei blood stages
    Generation of plasmids and transgenic P. <t>berghei</t> parasites for use in dual-luciferase assays. (A) The vector pFL 103464 RL ef1α with FL under the control of the promoter region 103464 and RL under the control of the ef1α promoter was generated and its transfection resulted in the parasite line PbFL 103464 RL ef1α . The plasmid pFL ef1α RL ef1α with FL and RL under the control of the ef1α promoter was used to obtain control parasites PbFL ef1α RL ef1α . Diamonds represent 3′UTRs, which in all cases were from the pbdhfr/ts gene. In the upper plasmid, the selection marker ( tgdhfr/ts ) is displayed but for simplicity in all other plasmid diagrams only those genes and features directly related to the experiments described are displayed. (B) Comparison of the luciferase activity (FL expression relative to RL expression) of transgenic parasites during the blood stage (BS), in oocysts (Oo), in salivary gland sporozoites (Sp) and in vitro in the liver stage (LS), 48 hours post-infection (hpi) of hepatoma cells. Standard deviation values (shown as error bars) were determined from three different measurements. Statistical analysis was performed using two-tailed unpaired t-tests (*P
    P Berghei Blood Stages, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p berghei blood stages/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    p berghei blood stages - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    97
    BioLegend pe conjugated anti human cd4
    Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in <t>CD4</t> + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P
    Pe Conjugated Anti Human Cd4, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe conjugated anti human cd4/product/BioLegend
    Average 97 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pe conjugated anti human cd4 - by Bioz Stars, 2020-05
    97/100 stars
      Buy from Supplier

    99
    Millipore blood uric acid
    Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in <t>CD4</t> + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P
    Blood Uric Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blood uric acid/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    blood uric acid - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    InvivoGen r848
    HCQ-mediated inhibition of IFNα production from pDCs in response to CpG-A and ssRNA, but not to <t>R848,</t> can be detected after isolation of PBMC from whole blood pre-treated with HCQ. Whole blood samples from healthy donors were treated with HCQ (1000 ng/ml) or not for 1 h prior to PBMC isolation and then stimulated with CpG-A (10 μM), R848 (1 μM) or ssRNA (4 μg/ml) for 6h. IFNα was analyzed by intracellular cytokine staining. (A) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate. (B) Percentages of IFNα-producing pDCs detected as shown in A ) after CpG-A stimulation ( n = 5 donors), R848 stimulation ( n = 3 donors), or ssRNA ( n = 5 donors) from PBMC isolated from whole blood pre-treated with HCQ or not. Statistical significance was assessed using two-tailed paired Student's t-test (* p
    R848, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 2630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r848/product/InvivoGen
    Average 99 stars, based on 2630 article reviews
    Price from $9.99 to $1999.99
    r848 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    85
    Diagnostic Products Corp coat a count progesterone invitro
    HCQ-mediated inhibition of IFNα production from pDCs in response to CpG-A and ssRNA, but not to <t>R848,</t> can be detected after isolation of PBMC from whole blood pre-treated with HCQ. Whole blood samples from healthy donors were treated with HCQ (1000 ng/ml) or not for 1 h prior to PBMC isolation and then stimulated with CpG-A (10 μM), R848 (1 μM) or ssRNA (4 μg/ml) for 6h. IFNα was analyzed by intracellular cytokine staining. (A) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate. (B) Percentages of IFNα-producing pDCs detected as shown in A ) after CpG-A stimulation ( n = 5 donors), R848 stimulation ( n = 3 donors), or ssRNA ( n = 5 donors) from PBMC isolated from whole blood pre-treated with HCQ or not. Statistical significance was assessed using two-tailed paired Student's t-test (* p
    Coat A Count Progesterone Invitro, supplied by Diagnostic Products Corp, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coat a count progesterone invitro/product/Diagnostic Products Corp
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    coat a count progesterone invitro - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    99
    Thermo Fisher mueller hinton agar
    HCQ-mediated inhibition of IFNα production from pDCs in response to CpG-A and ssRNA, but not to <t>R848,</t> can be detected after isolation of PBMC from whole blood pre-treated with HCQ. Whole blood samples from healthy donors were treated with HCQ (1000 ng/ml) or not for 1 h prior to PBMC isolation and then stimulated with CpG-A (10 μM), R848 (1 μM) or ssRNA (4 μg/ml) for 6h. IFNα was analyzed by intracellular cytokine staining. (A) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate. (B) Percentages of IFNα-producing pDCs detected as shown in A ) after CpG-A stimulation ( n = 5 donors), R848 stimulation ( n = 3 donors), or ssRNA ( n = 5 donors) from PBMC isolated from whole blood pre-treated with HCQ or not. Statistical significance was assessed using two-tailed paired Student's t-test (* p
    Mueller Hinton Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1705 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mueller hinton agar/product/Thermo Fisher
    Average 99 stars, based on 1705 article reviews
    Price from $9.99 to $1999.99
    mueller hinton agar - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    91
    Beckman Coulter hemoccult rapid diagnostic test kit
    HCQ-mediated inhibition of IFNα production from pDCs in response to CpG-A and ssRNA, but not to <t>R848,</t> can be detected after isolation of PBMC from whole blood pre-treated with HCQ. Whole blood samples from healthy donors were treated with HCQ (1000 ng/ml) or not for 1 h prior to PBMC isolation and then stimulated with CpG-A (10 μM), R848 (1 μM) or ssRNA (4 μg/ml) for 6h. IFNα was analyzed by intracellular cytokine staining. (A) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate. (B) Percentages of IFNα-producing pDCs detected as shown in A ) after CpG-A stimulation ( n = 5 donors), R848 stimulation ( n = 3 donors), or ssRNA ( n = 5 donors) from PBMC isolated from whole blood pre-treated with HCQ or not. Statistical significance was assessed using two-tailed paired Student's t-test (* p
    Hemoccult Rapid Diagnostic Test Kit, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hemoccult rapid diagnostic test kit/product/Beckman Coulter
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hemoccult rapid diagnostic test kit - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Assembly of DVD-ADCs and in vitro efficacy. a Structure of β-lactam MMAF. b anti-HER2 DVD-ADC was assembled by incubating the anti-HER2 DVD with four equivalents (eq) of β-lactam MMAF at room temperature (rt) for 4 h. Drug attachment (red star) occurs at the reactive Lys of h38C2 (yellow circle), located in the heavy chain variable domain of anti-HER2 DVD, to form a stable amide bond. c ESI-MS was performed with unconjugated anti-HER2 DVD and conjugated anti-HER2 DVD-ADC after reduction (10 mM DTT) and deglycosylation (PNGase F). No conjugation was detected on the light chain and the increase in mass of the heavy chain (~1160 Da) corresponds to the addition of exactly one eq of β-lactam MMAF. No unconjugated or higher drug loaded species were detected. d Cytotoxicity of anti-HER2 DVD-ADC (red) following incubation with HER2+ BC cell line SK-BR-3 and HER2− BC cell line MDA-MB-468 for 72 h at 37 °C (mean ± SD of triplicates). Unconjugated anti-HER2 DVD (blue) was significantly less toxic to SK-BR-3 cells when compared to anti-HER2 DVD-ADC ( p

    Journal: Nature Communications

    Article Title: Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates

    doi: 10.1038/s41467-017-01257-1

    Figure Lengend Snippet: Assembly of DVD-ADCs and in vitro efficacy. a Structure of β-lactam MMAF. b anti-HER2 DVD-ADC was assembled by incubating the anti-HER2 DVD with four equivalents (eq) of β-lactam MMAF at room temperature (rt) for 4 h. Drug attachment (red star) occurs at the reactive Lys of h38C2 (yellow circle), located in the heavy chain variable domain of anti-HER2 DVD, to form a stable amide bond. c ESI-MS was performed with unconjugated anti-HER2 DVD and conjugated anti-HER2 DVD-ADC after reduction (10 mM DTT) and deglycosylation (PNGase F). No conjugation was detected on the light chain and the increase in mass of the heavy chain (~1160 Da) corresponds to the addition of exactly one eq of β-lactam MMAF. No unconjugated or higher drug loaded species were detected. d Cytotoxicity of anti-HER2 DVD-ADC (red) following incubation with HER2+ BC cell line SK-BR-3 and HER2− BC cell line MDA-MB-468 for 72 h at 37 °C (mean ± SD of triplicates). Unconjugated anti-HER2 DVD (blue) was significantly less toxic to SK-BR-3 cells when compared to anti-HER2 DVD-ADC ( p

    Article Snippet: In vitro plasma stability assay Anti-HER2 DVD-ADC was diluted to 1 µM using reconstituted human plasma (Sigma-Aldrich) (94% (v/v) human plasma in PBS).

    Techniques: In Vitro, Mass Spectrometry, Conjugation Assay, Incubation, Multiple Displacement Amplification

    In vivo efficacy of anti-HER2 DVD-ADC. a Human BC cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm 3 , randomized into five groups comprising seven or eight mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls once a week for 4 weeks. The benchmark ADC, T-DM1 (black), was used as a positive control. Mean ± SEM values are plotted. When compared with a t -test (one-tailed, unpaired, uneven variance), the 5 mg kg −1 (red) and 10 mg kg −1 (blue) DVD-ADC groups were significantly different from the 10 mg kg −1 DVD group (green) on day 8 ( p = 0.0046 and p = 0.00016, respectively) until the last common data point on day 36 ( p = 0.0000069 and p = 0.0000019, respectively). b Corresponding Kaplan−Meier survival curve with p -values (log-rank (Mantel-Cox) test) comparing survival between anti-HER2 DVD-ADC groups (red and blue) and the unconjugated anti-HER2 DVD group (green)

    Journal: Nature Communications

    Article Title: Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates

    doi: 10.1038/s41467-017-01257-1

    Figure Lengend Snippet: In vivo efficacy of anti-HER2 DVD-ADC. a Human BC cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm 3 , randomized into five groups comprising seven or eight mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls once a week for 4 weeks. The benchmark ADC, T-DM1 (black), was used as a positive control. Mean ± SEM values are plotted. When compared with a t -test (one-tailed, unpaired, uneven variance), the 5 mg kg −1 (red) and 10 mg kg −1 (blue) DVD-ADC groups were significantly different from the 10 mg kg −1 DVD group (green) on day 8 ( p = 0.0046 and p = 0.00016, respectively) until the last common data point on day 36 ( p = 0.0000069 and p = 0.0000019, respectively). b Corresponding Kaplan−Meier survival curve with p -values (log-rank (Mantel-Cox) test) comparing survival between anti-HER2 DVD-ADC groups (red and blue) and the unconjugated anti-HER2 DVD group (green)

    Article Snippet: In vitro plasma stability assay Anti-HER2 DVD-ADC was diluted to 1 µM using reconstituted human plasma (Sigma-Aldrich) (94% (v/v) human plasma in PBS).

    Techniques: In Vivo, Mouse Assay, Positive Control, One-tailed Test

    Construction of anti-HER2 DVDs. a h38C2 IgG1 compared to an anti-HER2 DVD. The DVD is composed of variable domains of trastuzumab (blue), h38C2 (green) with reactive Lys (yellow circle), and constant domains (gray). Two fully human spacer sequences (red lines) were used to prepare DVD1 with a short spacer (ASTKGP) or DVD2 with a long spacer (TVAAPSVFIFPP). b Coomassie stained SDS-PAGE confirmed the purity of both DVD1 and DVD2 under non-reducing (expected ~200 kDa) and reducing conditions (expected heavy chain ~63 kDa, light chain ~36 kDa). Molecular weights from a pre-stained protein ladder are shown on the left. c Flow cytometry showing specific binding of DVD1 (red) and DVD2 (blue) to SK-BR-3 cells (HER2+) with no binding detected to MDA-MB-468 cells (HER2−). Trastuzumab IgG1 (green) was used as a positive control and h38C2 IgG1 (black) as a negative control. d The catalytic retro-aldol activity of the reactive Lys of h38C2 was measured using methodol as a substrate. The signal is reported in relative fluorescent units (RFU; mean ± SD of triplicates). Trastuzumab IgG1 (green) was used as a negative control. The slope of DVD1 was not significantly different ( p = 0.1967; linear regression analysis) from h38C2 IgG1, but the slope of DVD2 was significantly smaller ( p

    Journal: Nature Communications

    Article Title: Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates

    doi: 10.1038/s41467-017-01257-1

    Figure Lengend Snippet: Construction of anti-HER2 DVDs. a h38C2 IgG1 compared to an anti-HER2 DVD. The DVD is composed of variable domains of trastuzumab (blue), h38C2 (green) with reactive Lys (yellow circle), and constant domains (gray). Two fully human spacer sequences (red lines) were used to prepare DVD1 with a short spacer (ASTKGP) or DVD2 with a long spacer (TVAAPSVFIFPP). b Coomassie stained SDS-PAGE confirmed the purity of both DVD1 and DVD2 under non-reducing (expected ~200 kDa) and reducing conditions (expected heavy chain ~63 kDa, light chain ~36 kDa). Molecular weights from a pre-stained protein ladder are shown on the left. c Flow cytometry showing specific binding of DVD1 (red) and DVD2 (blue) to SK-BR-3 cells (HER2+) with no binding detected to MDA-MB-468 cells (HER2−). Trastuzumab IgG1 (green) was used as a positive control and h38C2 IgG1 (black) as a negative control. d The catalytic retro-aldol activity of the reactive Lys of h38C2 was measured using methodol as a substrate. The signal is reported in relative fluorescent units (RFU; mean ± SD of triplicates). Trastuzumab IgG1 (green) was used as a negative control. The slope of DVD1 was not significantly different ( p = 0.1967; linear regression analysis) from h38C2 IgG1, but the slope of DVD2 was significantly smaller ( p

    Article Snippet: In vitro plasma stability assay Anti-HER2 DVD-ADC was diluted to 1 µM using reconstituted human plasma (Sigma-Aldrich) (94% (v/v) human plasma in PBS).

    Techniques: Staining, SDS Page, Flow Cytometry, Cytometry, Binding Assay, Multiple Displacement Amplification, Positive Control, Negative Control, Activity Assay

    Performance in identification of polymicrobial blood cultures with the FilmArray BCID panel

    Journal: GMS Infectious Diseases

    Article Title: Performance of the FilmArray Blood culture identification panel in positive blood culture bottles and cerebrospinal fluid for the diagnosis of sepsis and meningitis

    doi: 10.3205/id000024

    Figure Lengend Snippet: Performance in identification of polymicrobial blood cultures with the FilmArray BCID panel

    Article Snippet: The recently introduced FilmArray blood culture identification (BCID) panel (BioFire, Salt Lake City, UT) an in vitro diagnostic (IVD)/Conformité Européene (CE)-labeled test system is a promising method for the use in positive blood cultures [ ], [ ].

    Techniques:

    Identification of pathogens and antibiotic resistance marker with culture and FilmArray BCID panel from positive blood cultures and cerebrospinal fluid samples

    Journal: GMS Infectious Diseases

    Article Title: Performance of the FilmArray Blood culture identification panel in positive blood culture bottles and cerebrospinal fluid for the diagnosis of sepsis and meningitis

    doi: 10.3205/id000024

    Figure Lengend Snippet: Identification of pathogens and antibiotic resistance marker with culture and FilmArray BCID panel from positive blood cultures and cerebrospinal fluid samples

    Article Snippet: The recently introduced FilmArray blood culture identification (BCID) panel (BioFire, Salt Lake City, UT) an in vitro diagnostic (IVD)/Conformité Européene (CE)-labeled test system is a promising method for the use in positive blood cultures [ ], [ ].

    Techniques: Marker

    In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein occludin. (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Mechanical Injury Induces Brain Endothelial-Derived Microvesicle Release: Implications for Cerebral Vascular Injury during Traumatic Brain Injury

    doi: 10.3389/fncel.2016.00043

    Figure Lengend Snippet: In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein occludin. (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p

    Article Snippet: After isolation, eMVs were centrifuged at 1500 × g for 30 min and resuspended in 100 μL of flow cytometry buffer with primary antibody Occludin (abcam) for 1 h. FITC conjugated Secondary antibody (eBioscience) was added for 30 min. 200 μL of flow cytometry buffer was added and the samples were analyzed by flow cytometry.

    Techniques: In Vitro, Marker, Isolation, Western Blot

    Experimental TBI induces the release of eMVs containing the tight junction protein occludin . Mice were subjected to sham surgery or a Controlled Cortical Impact, CCI-TBI and blood was collected 24 h post-injury. (A) Flow Cytometry detection of occludin (FITC) positive eMVs from blood plasma. Regions of interest were drawn around all FITC positive vesicles as well as a clear high FSC population. (B,C) TBI induces an increase in total FITC positive and high FSC-FITC positive vesicles at 24 h post-injury (Students two-tailed t -test average ± SEM n = 5 for sham and n = 4 for TBI, * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Mechanical Injury Induces Brain Endothelial-Derived Microvesicle Release: Implications for Cerebral Vascular Injury during Traumatic Brain Injury

    doi: 10.3389/fncel.2016.00043

    Figure Lengend Snippet: Experimental TBI induces the release of eMVs containing the tight junction protein occludin . Mice were subjected to sham surgery or a Controlled Cortical Impact, CCI-TBI and blood was collected 24 h post-injury. (A) Flow Cytometry detection of occludin (FITC) positive eMVs from blood plasma. Regions of interest were drawn around all FITC positive vesicles as well as a clear high FSC population. (B,C) TBI induces an increase in total FITC positive and high FSC-FITC positive vesicles at 24 h post-injury (Students two-tailed t -test average ± SEM n = 5 for sham and n = 4 for TBI, * p

    Article Snippet: After isolation, eMVs were centrifuged at 1500 × g for 30 min and resuspended in 100 μL of flow cytometry buffer with primary antibody Occludin (abcam) for 1 h. FITC conjugated Secondary antibody (eBioscience) was added for 30 min. 200 μL of flow cytometry buffer was added and the samples were analyzed by flow cytometry.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Two Tailed Test

    Generation of plasmids and transgenic P. berghei parasites for use in dual-luciferase assays. (A) The vector pFL 103464 RL ef1α with FL under the control of the promoter region 103464 and RL under the control of the ef1α promoter was generated and its transfection resulted in the parasite line PbFL 103464 RL ef1α . The plasmid pFL ef1α RL ef1α with FL and RL under the control of the ef1α promoter was used to obtain control parasites PbFL ef1α RL ef1α . Diamonds represent 3′UTRs, which in all cases were from the pbdhfr/ts gene. In the upper plasmid, the selection marker ( tgdhfr/ts ) is displayed but for simplicity in all other plasmid diagrams only those genes and features directly related to the experiments described are displayed. (B) Comparison of the luciferase activity (FL expression relative to RL expression) of transgenic parasites during the blood stage (BS), in oocysts (Oo), in salivary gland sporozoites (Sp) and in vitro in the liver stage (LS), 48 hours post-infection (hpi) of hepatoma cells. Standard deviation values (shown as error bars) were determined from three different measurements. Statistical analysis was performed using two-tailed unpaired t-tests (*P

    Journal: PLoS ONE

    Article Title: Identification and Characterization of a Liver Stage-Specific Promoter Region of the Malaria Parasite Plasmodium

    doi: 10.1371/journal.pone.0013653

    Figure Lengend Snippet: Generation of plasmids and transgenic P. berghei parasites for use in dual-luciferase assays. (A) The vector pFL 103464 RL ef1α with FL under the control of the promoter region 103464 and RL under the control of the ef1α promoter was generated and its transfection resulted in the parasite line PbFL 103464 RL ef1α . The plasmid pFL ef1α RL ef1α with FL and RL under the control of the ef1α promoter was used to obtain control parasites PbFL ef1α RL ef1α . Diamonds represent 3′UTRs, which in all cases were from the pbdhfr/ts gene. In the upper plasmid, the selection marker ( tgdhfr/ts ) is displayed but for simplicity in all other plasmid diagrams only those genes and features directly related to the experiments described are displayed. (B) Comparison of the luciferase activity (FL expression relative to RL expression) of transgenic parasites during the blood stage (BS), in oocysts (Oo), in salivary gland sporozoites (Sp) and in vitro in the liver stage (LS), 48 hours post-infection (hpi) of hepatoma cells. Standard deviation values (shown as error bars) were determined from three different measurements. Statistical analysis was performed using two-tailed unpaired t-tests (*P

    Article Snippet: For live imaging of P. berghei blood stages, tail blood was taken from an infected mouse, mixed with room temperature cMEM (see above) containing 1 µg/ml Hoechst 33342 (Sigma) and microscopically analyzed.

    Techniques: Transgenic Assay, Luciferase, Plasmid Preparation, Generated, Transfection, Selection, Marker, Activity Assay, Expressing, In Vitro, Infection, Standard Deviation, Two Tailed Test

    Promoter-dependent GFP expression. (A) The vector pGFP 103464 with GFP under the control of the promoter region 103464 and the vector pGFP ef1α with GFP under control of the ef1α promoter were generated and their transfection resulted in the parasite lines PbGFP 103464 and PbGFP ef1α . (B) Live imaging of PbGFP ef1α and PbGFP 103464 parasites at different life cycle stages. HepG2 cells were infected with transgenic P. berghei sporozoites and analyzed at different time points after infection (hpi, hours post-infection). GFP expresion was monitored by fluorescent microscopy. DNA was stained with Hoechst 33342. Arrows indicate young liver stage parasites. (iRBC: infected red blood cell; LS: liver stage) Scale bars: 10 µm.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of a Liver Stage-Specific Promoter Region of the Malaria Parasite Plasmodium

    doi: 10.1371/journal.pone.0013653

    Figure Lengend Snippet: Promoter-dependent GFP expression. (A) The vector pGFP 103464 with GFP under the control of the promoter region 103464 and the vector pGFP ef1α with GFP under control of the ef1α promoter were generated and their transfection resulted in the parasite lines PbGFP 103464 and PbGFP ef1α . (B) Live imaging of PbGFP ef1α and PbGFP 103464 parasites at different life cycle stages. HepG2 cells were infected with transgenic P. berghei sporozoites and analyzed at different time points after infection (hpi, hours post-infection). GFP expresion was monitored by fluorescent microscopy. DNA was stained with Hoechst 33342. Arrows indicate young liver stage parasites. (iRBC: infected red blood cell; LS: liver stage) Scale bars: 10 µm.

    Article Snippet: For live imaging of P. berghei blood stages, tail blood was taken from an infected mouse, mixed with room temperature cMEM (see above) containing 1 µg/ml Hoechst 33342 (Sigma) and microscopically analyzed.

    Techniques: Expressing, Plasmid Preparation, Generated, Transfection, Imaging, Infection, Transgenic Assay, Microscopy, Staining

    Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in CD4 + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P

    Journal: Cellular and Molecular Immunology

    Article Title: Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients

    doi: 10.1038/cmi.2018.5

    Figure Lengend Snippet: Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in CD4 + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P

    Article Snippet: Whole blood was stained with PE-conjugated anti-human CD4 (RPTA-4; BioLegend) and FITC-conjugated anti-human CD45RA (clone HI100; BioLegend) for 30 min on ice in the dark.

    Techniques: Expressing, In Vitro, Fluorescence, Staining, MANN-WHITNEY, Two Tailed Test

    M. tuberculosis -specific activation and intracellular cytokine expression in association with SOCS3 expression in both study groups. ( a ) Representative graphs depicting the gating procedure for intracellular cytokine analysis are shown. ( b ) Proportions of CD40L/IL-2-positive CD4 + T-cells are shown for tuberculosis patients (grey squares) and healthy contacts (open circles). ( c ) Correlation plots between SOCS3 expression and CD40L/IL-2 (upper graph) as well as CD40L/IFN-γ (lower graph) are shown for CD4 + T-cells. The Spearman Rank test was used to determine significant correlations for all donors and both study groups separately. Correlation coefficients (rho) and nominal P -values are given. ns: not significant.

    Journal: Cellular and Molecular Immunology

    Article Title: Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients

    doi: 10.1038/cmi.2018.5

    Figure Lengend Snippet: M. tuberculosis -specific activation and intracellular cytokine expression in association with SOCS3 expression in both study groups. ( a ) Representative graphs depicting the gating procedure for intracellular cytokine analysis are shown. ( b ) Proportions of CD40L/IL-2-positive CD4 + T-cells are shown for tuberculosis patients (grey squares) and healthy contacts (open circles). ( c ) Correlation plots between SOCS3 expression and CD40L/IL-2 (upper graph) as well as CD40L/IFN-γ (lower graph) are shown for CD4 + T-cells. The Spearman Rank test was used to determine significant correlations for all donors and both study groups separately. Correlation coefficients (rho) and nominal P -values are given. ns: not significant.

    Article Snippet: Whole blood was stained with PE-conjugated anti-human CD4 (RPTA-4; BioLegend) and FITC-conjugated anti-human CD45RA (clone HI100; BioLegend) for 30 min on ice in the dark.

    Techniques: Activation Assay, Expressing

    HCQ-mediated inhibition of IFNα production from pDCs in response to CpG-A and ssRNA, but not to R848, can be detected after isolation of PBMC from whole blood pre-treated with HCQ. Whole blood samples from healthy donors were treated with HCQ (1000 ng/ml) or not for 1 h prior to PBMC isolation and then stimulated with CpG-A (10 μM), R848 (1 μM) or ssRNA (4 μg/ml) for 6h. IFNα was analyzed by intracellular cytokine staining. (A) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate. (B) Percentages of IFNα-producing pDCs detected as shown in A ) after CpG-A stimulation ( n = 5 donors), R848 stimulation ( n = 3 donors), or ssRNA ( n = 5 donors) from PBMC isolated from whole blood pre-treated with HCQ or not. Statistical significance was assessed using two-tailed paired Student's t-test (* p

    Journal: Frontiers in Immunology

    Article Title: Effect of in vivo Hydroxychloroquine and ex vivo Anti-BDCA2 mAb Treatment on pDC IFNα Production From Patients Affected With Cutaneous Lupus Erythematosus

    doi: 10.3389/fimmu.2019.00275

    Figure Lengend Snippet: HCQ-mediated inhibition of IFNα production from pDCs in response to CpG-A and ssRNA, but not to R848, can be detected after isolation of PBMC from whole blood pre-treated with HCQ. Whole blood samples from healthy donors were treated with HCQ (1000 ng/ml) or not for 1 h prior to PBMC isolation and then stimulated with CpG-A (10 μM), R848 (1 μM) or ssRNA (4 μg/ml) for 6h. IFNα was analyzed by intracellular cytokine staining. (A) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate. (B) Percentages of IFNα-producing pDCs detected as shown in A ) after CpG-A stimulation ( n = 5 donors), R848 stimulation ( n = 3 donors), or ssRNA ( n = 5 donors) from PBMC isolated from whole blood pre-treated with HCQ or not. Statistical significance was assessed using two-tailed paired Student's t-test (* p

    Article Snippet: Together these data indicated that, in vitro , clinically-relevant concentrations of HCQ are more effective at inhibiting CpG-A-induced IFNα from whole blood compared to R848 and ssRNA induced-IFNα.

    Techniques: Inhibition, Isolation, Staining, Two Tailed Test

    24F4A further reduces pDC IFNα production after CpG-A, R848 or ssRNA stimulations of PBMC isolated from CLE patients with blood IFN-high signature or concomitant SLE diagnostic. (A) Distribution of the blood IFN signature scores in healthy individuals ( n = 52) and CLE patients ( n = 30). (B) Comparison of SLEDAI2-K disease scores (left) or CLASI skin scores (right) between CLE patients with blood IFN-low and blood IFN-high signature scores. Statistical significance was assessed using Mann-Whitney test (* p

    Journal: Frontiers in Immunology

    Article Title: Effect of in vivo Hydroxychloroquine and ex vivo Anti-BDCA2 mAb Treatment on pDC IFNα Production From Patients Affected With Cutaneous Lupus Erythematosus

    doi: 10.3389/fimmu.2019.00275

    Figure Lengend Snippet: 24F4A further reduces pDC IFNα production after CpG-A, R848 or ssRNA stimulations of PBMC isolated from CLE patients with blood IFN-high signature or concomitant SLE diagnostic. (A) Distribution of the blood IFN signature scores in healthy individuals ( n = 52) and CLE patients ( n = 30). (B) Comparison of SLEDAI2-K disease scores (left) or CLASI skin scores (right) between CLE patients with blood IFN-low and blood IFN-high signature scores. Statistical significance was assessed using Mann-Whitney test (* p

    Article Snippet: Together these data indicated that, in vitro , clinically-relevant concentrations of HCQ are more effective at inhibiting CpG-A-induced IFNα from whole blood compared to R848 and ssRNA induced-IFNα.

    Techniques: Isolation, Diagnostic Assay, MANN-WHITNEY

    Effect of HCQ and BIIB059 on IFNα release from human whole blood stimulated with CpG-A, R848, or ssRNA. Whole blood from human healthy donors was stimulated or not with CpG-A (10 μM), R848 (1 μM), or ssRNA (4 μg/ml) complexed with pARG in presence or absence of HCQ (550 ng/ml), isotype control mAb (10 μg/ml), 24F4A (10 μg/ml) or a combination of both HCQ and 24F4A. Secreted IFNα was measured 18 h after stimulation in the serum using ELISA. Same color and/or shape-coded data points represent data obtained from a given donor ( n = at least 6 donors). Statistical tests were performed using paired one-way Anova (ns, non-significant p ≥ 0.05, * p

    Journal: Frontiers in Immunology

    Article Title: Effect of in vivo Hydroxychloroquine and ex vivo Anti-BDCA2 mAb Treatment on pDC IFNα Production From Patients Affected With Cutaneous Lupus Erythematosus

    doi: 10.3389/fimmu.2019.00275

    Figure Lengend Snippet: Effect of HCQ and BIIB059 on IFNα release from human whole blood stimulated with CpG-A, R848, or ssRNA. Whole blood from human healthy donors was stimulated or not with CpG-A (10 μM), R848 (1 μM), or ssRNA (4 μg/ml) complexed with pARG in presence or absence of HCQ (550 ng/ml), isotype control mAb (10 μg/ml), 24F4A (10 μg/ml) or a combination of both HCQ and 24F4A. Secreted IFNα was measured 18 h after stimulation in the serum using ELISA. Same color and/or shape-coded data points represent data obtained from a given donor ( n = at least 6 donors). Statistical tests were performed using paired one-way Anova (ns, non-significant p ≥ 0.05, * p

    Article Snippet: Together these data indicated that, in vitro , clinically-relevant concentrations of HCQ are more effective at inhibiting CpG-A-induced IFNα from whole blood compared to R848 and ssRNA induced-IFNα.

    Techniques: Enzyme-linked Immunosorbent Assay

    The percentage of IFNα-producing pDCs upon stimulation with CpG-A, but not R848 or ssRNA, is negatively correlated with whole blood HCQ concentrations from CLE patients. (A) Association of the percentage of IFNα-producing pDCs identified by flow cytometry from PBMC after CpG-A ( n = 22 donors), R848 ( n = 22 donors), or ssRNA ( n = 24 donors) stimulations with the HCQ concentrations in whole blood from CLE patients. Patients with non-detectable blood HCQ levels are shown at 0 ng/ml blood HCQ (B) Association of the change (ratio) between 3-month apart clinical visits in the percentage of IFNα-producing pDCs identified by flow cytometry from PBMC after CpG-A ( n = 21 donors), R848 ( n = 21 donors), or ssRNA ( n = 22 donors) stimulation with the change in the blood HCQ concentration of CLE patients. Data points from patients with increase in blood HCQ concentration > 600 ng/ml between the two visits are highlighted in red. Data points from patients with decrease in blood HCQ concentration > 500 ng/ml between two visits are highlighted in green. Statistical association was assessed using Pearson's (P) and Spearman's rank (S) correlations.

    Journal: Frontiers in Immunology

    Article Title: Effect of in vivo Hydroxychloroquine and ex vivo Anti-BDCA2 mAb Treatment on pDC IFNα Production From Patients Affected With Cutaneous Lupus Erythematosus

    doi: 10.3389/fimmu.2019.00275

    Figure Lengend Snippet: The percentage of IFNα-producing pDCs upon stimulation with CpG-A, but not R848 or ssRNA, is negatively correlated with whole blood HCQ concentrations from CLE patients. (A) Association of the percentage of IFNα-producing pDCs identified by flow cytometry from PBMC after CpG-A ( n = 22 donors), R848 ( n = 22 donors), or ssRNA ( n = 24 donors) stimulations with the HCQ concentrations in whole blood from CLE patients. Patients with non-detectable blood HCQ levels are shown at 0 ng/ml blood HCQ (B) Association of the change (ratio) between 3-month apart clinical visits in the percentage of IFNα-producing pDCs identified by flow cytometry from PBMC after CpG-A ( n = 21 donors), R848 ( n = 21 donors), or ssRNA ( n = 22 donors) stimulation with the change in the blood HCQ concentration of CLE patients. Data points from patients with increase in blood HCQ concentration > 600 ng/ml between the two visits are highlighted in red. Data points from patients with decrease in blood HCQ concentration > 500 ng/ml between two visits are highlighted in green. Statistical association was assessed using Pearson's (P) and Spearman's rank (S) correlations.

    Article Snippet: Together these data indicated that, in vitro , clinically-relevant concentrations of HCQ are more effective at inhibiting CpG-A-induced IFNα from whole blood compared to R848 and ssRNA induced-IFNα.

    Techniques: Flow Cytometry, Cytometry, Concentration Assay

    24F4A further reduces pDC IFNα production after CpG-A, R848 or ssRNA stimulations of PBMC isolated from CLE patients regardless blood HCQ levels. (A) Concentrations of HCQ in whole blood from the CLE patient cohort studied ( n = 30). (B) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate after CpG-A, R848 or ssRNA stimulation with or without pre-treatment with 24F4A (10 μg/ml 30 min) from CLE patient without detectable blood HCQ (top panel) or a CLE patient with a blood HCQ concentration of more than 500ng/ml (bottom panel). (C) Effect of 24F4A on the percentage of IFNα-producing pDCs induced by CpG-A, R848 and ssRNA stimulations and detected by flow cytometry in PBMC from CLE patients without detectable level of blood HCQ ( n ≥ 6 donors), with blood HCQ concentrations lower than 500 ng/ml ( n ≥ 8 donors) or > 500ng/ml ( n ≥ 6 donors). Statistical significance was assessed with a two-tailed paired Student's t-test using log2-transformed values (* p

    Journal: Frontiers in Immunology

    Article Title: Effect of in vivo Hydroxychloroquine and ex vivo Anti-BDCA2 mAb Treatment on pDC IFNα Production From Patients Affected With Cutaneous Lupus Erythematosus

    doi: 10.3389/fimmu.2019.00275

    Figure Lengend Snippet: 24F4A further reduces pDC IFNα production after CpG-A, R848 or ssRNA stimulations of PBMC isolated from CLE patients regardless blood HCQ levels. (A) Concentrations of HCQ in whole blood from the CLE patient cohort studied ( n = 30). (B) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate after CpG-A, R848 or ssRNA stimulation with or without pre-treatment with 24F4A (10 μg/ml 30 min) from CLE patient without detectable blood HCQ (top panel) or a CLE patient with a blood HCQ concentration of more than 500ng/ml (bottom panel). (C) Effect of 24F4A on the percentage of IFNα-producing pDCs induced by CpG-A, R848 and ssRNA stimulations and detected by flow cytometry in PBMC from CLE patients without detectable level of blood HCQ ( n ≥ 6 donors), with blood HCQ concentrations lower than 500 ng/ml ( n ≥ 8 donors) or > 500ng/ml ( n ≥ 6 donors). Statistical significance was assessed with a two-tailed paired Student's t-test using log2-transformed values (* p

    Article Snippet: Together these data indicated that, in vitro , clinically-relevant concentrations of HCQ are more effective at inhibiting CpG-A-induced IFNα from whole blood compared to R848 and ssRNA induced-IFNα.

    Techniques: Isolation, Concentration Assay, Flow Cytometry, Cytometry, Two Tailed Test, Transformation Assay