in vitro transcription translation ivt reaction Search Results


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    Promega in vitro transcription translation reactions ivt
    Mad2-binding motif peptides and Tyc1 disrupt co-factor binding to APC/C and display an additive phenotype for microtubule poison sensitivity when co-ever expressed. A) 35 S-Methionine radio-labeled full-length Cdc20 or Cdh1 were made by <t>IVT/T</t> and pre-incubated with buffer only as a control, or Tyc1, DQ36 or PQ65 at a final concentration of 220 μM. Samples were mixed with enriched APC/C isolated on IgG beads and allowed to bind for 1 hour. Samples were washed and beads were boiled directly in protein sample buffer. The amount of APC/C-bound co-factor was measured by phosphor-imaging (n = 3). B) Co-over expression of PQ65 with Tyc1 in vivo leads to an increased sensitivity to the microtubule poison benomyl. In the presence of 12.5 μg/mL benomyl a significant decrease in the average colony number was observed (n = 5).
    In Vitro Transcription Translation Reactions Ivt, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher human cell free expression system reaction
    Mad2-binding motif peptides and Tyc1 disrupt co-factor binding to APC/C and display an additive phenotype for microtubule poison sensitivity when co-ever expressed. A) 35 S-Methionine radio-labeled full-length Cdc20 or Cdh1 were made by <t>IVT/T</t> and pre-incubated with buffer only as a control, or Tyc1, DQ36 or PQ65 at a final concentration of 220 μM. Samples were mixed with enriched APC/C isolated on IgG beads and allowed to bind for 1 hour. Samples were washed and beads were boiled directly in protein sample buffer. The amount of APC/C-bound co-factor was measured by phosphor-imaging (n = 3). B) Co-over expression of PQ65 with Tyc1 in vivo leads to an increased sensitivity to the microtubule poison benomyl. In the presence of 12.5 μg/mL benomyl a significant decrease in the average colony number was observed (n = 5).
    Human Cell Free Expression System Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cell free expression system reaction/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human cell free expression system reaction - by Bioz Stars, 2022-12
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    91
    New England Biolabs in vitro transcription translation ivt reaction
    Mad2-binding motif peptides and Tyc1 disrupt co-factor binding to APC/C and display an additive phenotype for microtubule poison sensitivity when co-ever expressed. A) 35 S-Methionine radio-labeled full-length Cdc20 or Cdh1 were made by <t>IVT/T</t> and pre-incubated with buffer only as a control, or Tyc1, DQ36 or PQ65 at a final concentration of 220 μM. Samples were mixed with enriched APC/C isolated on IgG beads and allowed to bind for 1 hour. Samples were washed and beads were boiled directly in protein sample buffer. The amount of APC/C-bound co-factor was measured by phosphor-imaging (n = 3). B) Co-over expression of PQ65 with Tyc1 in vivo leads to an increased sensitivity to the microtubule poison benomyl. In the presence of 12.5 μg/mL benomyl a significant decrease in the average colony number was observed (n = 5).
    In Vitro Transcription Translation Ivt Reaction, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro transcription translation ivt reaction/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in vitro transcription translation ivt reaction - by Bioz Stars, 2022-12
    91/100 stars
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    Mad2-binding motif peptides and Tyc1 disrupt co-factor binding to APC/C and display an additive phenotype for microtubule poison sensitivity when co-ever expressed. A) 35 S-Methionine radio-labeled full-length Cdc20 or Cdh1 were made by IVT/T and pre-incubated with buffer only as a control, or Tyc1, DQ36 or PQ65 at a final concentration of 220 μM. Samples were mixed with enriched APC/C isolated on IgG beads and allowed to bind for 1 hour. Samples were washed and beads were boiled directly in protein sample buffer. The amount of APC/C-bound co-factor was measured by phosphor-imaging (n = 3). B) Co-over expression of PQ65 with Tyc1 in vivo leads to an increased sensitivity to the microtubule poison benomyl. In the presence of 12.5 μg/mL benomyl a significant decrease in the average colony number was observed (n = 5).

    Journal: PLoS ONE

    Article Title: Peptide inhibitors of the anaphase promoting-complex that cause sensitivity to microtubule poison

    doi: 10.1371/journal.pone.0198930

    Figure Lengend Snippet: Mad2-binding motif peptides and Tyc1 disrupt co-factor binding to APC/C and display an additive phenotype for microtubule poison sensitivity when co-ever expressed. A) 35 S-Methionine radio-labeled full-length Cdc20 or Cdh1 were made by IVT/T and pre-incubated with buffer only as a control, or Tyc1, DQ36 or PQ65 at a final concentration of 220 μM. Samples were mixed with enriched APC/C isolated on IgG beads and allowed to bind for 1 hour. Samples were washed and beads were boiled directly in protein sample buffer. The amount of APC/C-bound co-factor was measured by phosphor-imaging (n = 3). B) Co-over expression of PQ65 with Tyc1 in vivo leads to an increased sensitivity to the microtubule poison benomyl. In the presence of 12.5 μg/mL benomyl a significant decrease in the average colony number was observed (n = 5).

    Article Snippet: 35 S-Pds1 was prepared in a 500 μL IVT/T (In Vitro Transcription/Translation) reaction (Promega, USA), which contained 400 μL rabbit reticulocyte lysate, 20 μL of L-35 S-methionine (PerkinElmer, USA), 10 μg of plasmid DNA, and the remaining volume as nuclease-free ddH2 O as recommended by the manufacturer.

    Techniques: Binding Assay, Labeling, Incubation, Concentration Assay, Isolation, Imaging, Over Expression, In Vivo