in vitro run-off transcription Search Results


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  • 98
    New England Biolabs nsii
    Nsii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher megascript t7 kit
    Megascript T7 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7848 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher run off in vitro transcription
    Run Off In Vitro Transcription, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche run off in vitro transcription
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    Run Off In Vitro Transcription, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega in vitro run off transcription reaction
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    In Vitro Run Off Transcription Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche t7 rnap
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    T7 Rnap, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega riboprobe in vitro transcription system
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    Riboprobe In Vitro Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher megashortscript transcription kit
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    Megashortscript Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy minielute cleanup kit
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    Rneasy Minielute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mmessage mmachine sp6 transcription kit
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    Mmessage Mmachine Sp6 Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t3 mmessage mmachine kit
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    T3 Mmessage Mmachine Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t3 rna polymerase
    Outline of the first subtractive step. Synthetic RNAs were produced by <t>run-off</t> <t>in</t> <t>vitro</t> <t>transcription</t> from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.
    T3 Rna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 1472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t7 rna polymerase
    Opposite co-transcriptional folding paths of a pair of RNA switches with ‘direct’ and ‘reverse’ sequences (i.e. 5′-ABCD-3′ versus 5′-DCBA-3′). Structures 1D and 1R (respectively, 2D and 2R) of the direct and reverse switches are energetically equivalent because of helix symmetries; dashed lines indicate mirror symmetry of Pa, Pb, Pc and Pd which are therefore conserved under sequence reversal relating direct and reverse switches. Despite these strong similarities between D and R structures at equilibrium, direct and reverse switches display ‘opposite’ co-transcriptional folding paths (direct switch into structure 1D and reverse switch into structure 2R) guided through a helix encoded persistence (left) or exchange (right) during in vitro transcription using <t>T7</t> RNA polymerase (see Materials and Methods).
    T7 Rna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3659 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche t3 rna polymerase
    Opposite co-transcriptional folding paths of a pair of RNA switches with ‘direct’ and ‘reverse’ sequences (i.e. 5′-ABCD-3′ versus 5′-DCBA-3′). Structures 1D and 1R (respectively, 2D and 2R) of the direct and reverse switches are energetically equivalent because of helix symmetries; dashed lines indicate mirror symmetry of Pa, Pb, Pc and Pd which are therefore conserved under sequence reversal relating direct and reverse switches. Despite these strong similarities between D and R structures at equilibrium, direct and reverse switches display ‘opposite’ co-transcriptional folding paths (direct switch into structure 1D and reverse switch into structure 2R) guided through a helix encoded persistence (left) or exchange (right) during in vitro transcription using <t>T7</t> RNA polymerase (see Materials and Methods).
    T3 Rna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 1095 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher maxiscript t7 t3 kit
    Opposite co-transcriptional folding paths of a pair of RNA switches with ‘direct’ and ‘reverse’ sequences (i.e. 5′-ABCD-3′ versus 5′-DCBA-3′). Structures 1D and 1R (respectively, 2D and 2R) of the direct and reverse switches are energetically equivalent because of helix symmetries; dashed lines indicate mirror symmetry of Pa, Pb, Pc and Pd which are therefore conserved under sequence reversal relating direct and reverse switches. Despite these strong similarities between D and R structures at equilibrium, direct and reverse switches display ‘opposite’ co-transcriptional folding paths (direct switch into structure 1D and reverse switch into structure 2R) guided through a helix encoded persistence (left) or exchange (right) during in vitro transcription using <t>T7</t> RNA polymerase (see Materials and Methods).
    Maxiscript T7 T3 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare α 32 p utp
    Opposite co-transcriptional folding paths of a pair of RNA switches with ‘direct’ and ‘reverse’ sequences (i.e. 5′-ABCD-3′ versus 5′-DCBA-3′). Structures 1D and 1R (respectively, 2D and 2R) of the direct and reverse switches are energetically equivalent because of helix symmetries; dashed lines indicate mirror symmetry of Pa, Pb, Pc and Pd which are therefore conserved under sequence reversal relating direct and reverse switches. Despite these strong similarities between D and R structures at equilibrium, direct and reverse switches display ‘opposite’ co-transcriptional folding paths (direct switch into structure 1D and reverse switch into structure 2R) guided through a helix encoded persistence (left) or exchange (right) during in vitro transcription using <t>T7</t> RNA polymerase (see Materials and Methods).
    α 32 P Utp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase kit
    Opposite co-transcriptional folding paths of a pair of RNA switches with ‘direct’ and ‘reverse’ sequences (i.e. 5′-ABCD-3′ versus 5′-DCBA-3′). Structures 1D and 1R (respectively, 2D and 2R) of the direct and reverse switches are energetically equivalent because of helix symmetries; dashed lines indicate mirror symmetry of Pa, Pb, Pc and Pd which are therefore conserved under sequence reversal relating direct and reverse switches. Despite these strong similarities between D and R structures at equilibrium, direct and reverse switches display ‘opposite’ co-transcriptional folding paths (direct switch into structure 1D and reverse switch into structure 2R) guided through a helix encoded persistence (left) or exchange (right) during in vitro transcription using <t>T7</t> RNA polymerase (see Materials and Methods).
    T7 Rna Polymerase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t7 rna polymerase promoters
    Scheme for the in vitro synthesis of the suppressor tRNA from a synthetic DNA template by T7 run-off transcription. Two synthetic complementary oligonucleotides encoding the E.coli -derived suppressor tRNA Ala CUA were annealed and cloned into the pCR4 vector, leading to pEcoliAlaCUA. <t>T7</t> RNA polymerase run-off transcription was performed on the purified Eco RI and Bsa I fragment giving rise to a 76mer suppressor tRNA.
    T7 Rna Polymerase Promoters, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 ribomax express large scale rna production
    Scheme for the in vitro synthesis of the suppressor tRNA from a synthetic DNA template by T7 run-off transcription. Two synthetic complementary oligonucleotides encoding the E.coli -derived suppressor tRNA Ala CUA were annealed and cloned into the pCR4 vector, leading to pEcoliAlaCUA. <t>T7</t> RNA polymerase run-off transcription was performed on the purified Eco RI and Bsa I fragment giving rise to a 76mer suppressor tRNA.
    T7 Ribomax Express Large Scale Rna Production, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Epicentre Biotechnologies e coli rna polymerase holoenzyme
    Scheme for the in vitro synthesis of the suppressor tRNA from a synthetic DNA template by T7 run-off transcription. Two synthetic complementary oligonucleotides encoding the E.coli -derived suppressor tRNA Ala CUA were annealed and cloned into the pCR4 vector, leading to pEcoliAlaCUA. <t>T7</t> RNA polymerase run-off transcription was performed on the purified Eco RI and Bsa I fragment giving rise to a 76mer suppressor tRNA.
    E Coli Rna Polymerase Holoenzyme, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Outline of the first subtractive step. Synthetic RNAs were produced by run-off in vitro transcription from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.

    Journal: Nucleic Acids Research

    Article Title: A two-step strategy for constructing specifically self-subtracted cDNA libraries

    doi:

    Figure Lengend Snippet: Outline of the first subtractive step. Synthetic RNAs were produced by run-off in vitro transcription from a 3′-end-specific cDNA library (HM3). The resulting RNA molecules are nearly uniform in length (400–600 nt) and their composition reflects the relative abundance of each cDNA in the starting library. Super-prevalent RNAs are shown as black wavy lines. We designed DNA oligonucleotides complementary to the 3′-UTR of the super-prevalent mRNAs, then RNAs and DNA oligonucleotides were incubated in the presence of RNase H, which specifically degrades the RNA moiety in a DNA:RNA hybrid. The undigested RNAs were purified from preparative gels, reverse transcribed and cloned in plasmid vectors.

    Article Snippet: Antisense, biotinylated RNA molecules were obtained by run-off in vitro transcription in the presence of biotin-16-UTP (Roche).

    Techniques: Produced, In Vitro, cDNA Library Assay, Incubation, Purification, Clone Assay, Plasmid Preparation

    Opposite co-transcriptional folding paths of a pair of RNA switches with ‘direct’ and ‘reverse’ sequences (i.e. 5′-ABCD-3′ versus 5′-DCBA-3′). Structures 1D and 1R (respectively, 2D and 2R) of the direct and reverse switches are energetically equivalent because of helix symmetries; dashed lines indicate mirror symmetry of Pa, Pb, Pc and Pd which are therefore conserved under sequence reversal relating direct and reverse switches. Despite these strong similarities between D and R structures at equilibrium, direct and reverse switches display ‘opposite’ co-transcriptional folding paths (direct switch into structure 1D and reverse switch into structure 2R) guided through a helix encoded persistence (left) or exchange (right) during in vitro transcription using T7 RNA polymerase (see Materials and Methods).

    Journal: Nucleic Acids Research

    Article Title: Encoding folding paths of RNA switches

    doi: 10.1093/nar/gkl1036

    Figure Lengend Snippet: Opposite co-transcriptional folding paths of a pair of RNA switches with ‘direct’ and ‘reverse’ sequences (i.e. 5′-ABCD-3′ versus 5′-DCBA-3′). Structures 1D and 1R (respectively, 2D and 2R) of the direct and reverse switches are energetically equivalent because of helix symmetries; dashed lines indicate mirror symmetry of Pa, Pb, Pc and Pd which are therefore conserved under sequence reversal relating direct and reverse switches. Despite these strong similarities between D and R structures at equilibrium, direct and reverse switches display ‘opposite’ co-transcriptional folding paths (direct switch into structure 1D and reverse switch into structure 2R) guided through a helix encoded persistence (left) or exchange (right) during in vitro transcription using T7 RNA polymerase (see Materials and Methods).

    Article Snippet: Run off transcription was performed in vitro using T7 RNA polymerase (New England Biolabs) for up to 4–5 h at 37 or 25°C.

    Techniques: Sequencing, In Vitro

    Scheme for the in vitro synthesis of the suppressor tRNA from a synthetic DNA template by T7 run-off transcription. Two synthetic complementary oligonucleotides encoding the E.coli -derived suppressor tRNA Ala CUA were annealed and cloned into the pCR4 vector, leading to pEcoliAlaCUA. T7 RNA polymerase run-off transcription was performed on the purified Eco RI and Bsa I fragment giving rise to a 76mer suppressor tRNA.

    Journal: Nucleic Acids Research

    Article Title: Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery

    doi:

    Figure Lengend Snippet: Scheme for the in vitro synthesis of the suppressor tRNA from a synthetic DNA template by T7 run-off transcription. Two synthetic complementary oligonucleotides encoding the E.coli -derived suppressor tRNA Ala CUA were annealed and cloned into the pCR4 vector, leading to pEcoliAlaCUA. T7 RNA polymerase run-off transcription was performed on the purified Eco RI and Bsa I fragment giving rise to a 76mer suppressor tRNA.

    Article Snippet: After agarose gel purification this DNA fragment was used for the run-off transcription with T7 RNA polymerase following the protocol of the MegaScript kit (Ambion).

    Techniques: In Vitro, Derivative Assay, Clone Assay, Plasmid Preparation, Purification