Journal: Journal of Bacteriology
Article Title: Identification of the Gene Encoding the Alternative Sigma Factor ?B from Listeria monocytogenes and Its Role in Osmotolerance †
Figure Lengend Snippet: Growth of wild-type and sigB mutant strains of L. monocytogenes after osmotic upshift in BHI (A) or DM (B and C). (A) LO4035 (wild type) and LMA2B ( sigB :: km ) were grown in BHI, and the cultures were divided into two equal portions at 425 min (arrow). Sodium chloride was added to one portion to a final concentration of 6%, and both portions were further incubated. Symbols: ■, LO4035 without NaCl; □, LO4035 with 6% NaCl; •, LMA2B without NaCl; ○, LMA2B with 6% NaCl. (B) Equivalent volumes of 18-h cultures of LO4035 and LMA2B in DM were inoculated into DM without NaCl (▴, LO4035; ■, LMA2B), DM with 3% NaCl (✕⃒, LO4035; ×, LMA2B), and DM with 3% NaCl supplemented with 1 mM betaine (▵, LO4035; □, LMA2B. (C) Equivalent volumes of 18-h cultures of LO4035 and LMA2B in DM were inoculated into fresh DM (▴, LO4035; ■, LMA2B), DM with 3% NaCl (✕⃒, LO4035; ×, LMA2B), and DM with 3% NaCl supplemented with 1 mM carnitine (▵, LO4035; □, LMA2B).
Article Snippet: L. monocytogenes cells were grown in brain heart infusion (BHI) (Difco, Detroit, Mich.) at 30°C unless otherwise specified.
Techniques: Mutagenesis, Concentration Assay, Incubation