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    CLS Cell Lines Service GmbH imr 32
    ( A ) Profiling TERRA expression in ALT+ and ALT- NB cell lines (with MYCN amplification [NMA] and without MYCN amplification [Non-NMA]) by slot blot assay performed with 50, 100, and 200 ng of RNA isolated from cell lines indicated. Blot probed with a DIG-labeled TERRA probe. TapeStation profile on the right shows 18s and 28s rRNA served as a loading control. Bar graph shows the TERRA level quantified from blots and normalized to 28s rRNA loading control. Data are shown as mean ± SD from three biological replicates. One-way ANOVA with Tukey's post hoc test was used, **** P < 0.0001. ( B ) m 6 A RIP followed by TERRA slot blot on RNA isolated from ALT+ and ALT-cell lines. RIP with IgG severed as a negative control. Bar graph shows the quantification of the blots. Data are shown as mean ± SD from three biological replicates. Sidak's multiple comparisons test was used, **** P < 0.0001; ns - nonsignificant P > 0.05. ( C ) TERRA foci (green) visualized by TERRA RNA-FISH in ALT+ (upper panel), NMA (middle panel) and Non-NMA (lower panel) NB cell lines. Box plot shows the quantification of the TERRA foci per nucleus. At least 67 cells were counted from three independent biological replicates. ( D ) TERRA foci (green) combined with m 6 A (red) IF in CHLA-90 (top) and SK-N-FI (bottom) cells. Box plot shows the number of overlaps of TERRA and m 6 A per nucleus. At least 65 cells were counted from three independent biological replicates. Scale bar is 10 μm. ( E ) The m 6 A enrichment profiles across all subtelomeres using T2T assembly in SK-N-FI and SK-N-BE cells. The m 6 A RIP/input signals were normalized to spike-in and to the mappability likelihood of each chromosome ends. ( F ) Box plot of spike-in, mappability-normalized m 6 A RIP/input enrichment profiles of all subtelomeres in SK-N-FI and SK-N-BE. Statistical significance was calculated using the Wilcoxon test, **** P < 0.0001. ( G , left panel) TERRA expression in ALT+, NMA and Non-NMA NB patient tumor RNA samples were detected by slot blot assay performed with 100 ng of total RNA. (Middle panel) TapeStation profile shows 18s and 28s rRNA served as a loading control, samples are loaded as NB1 to NB13 from top to bottom. (Right panel) Box plot shows the TERRA level quantified from blots and normalized to 28s rRNA loading control. Two-way ANOVA with Tukey's post hoc test was used, * P < 0.05; ns - nonsignificant P > 0.05. ( H ) Representative browser screenshot of 50 kb region around the telomere ends, showing TERRA RNA expression (CPM) from two of the active chromosome ends (Chr9p, Chr16q) and one inactive chromosome end (Chr3p) using Illumina short read RNA-seq performed on NB1 and NB2 tumor samples. CpG islands are marked with green bars and the telomeric repeats in this region are marked with black bars. ( I ) Heatmap summarizing RNA-seq and m 6 A RIP-seq data of two NB tumor samples (NB1 and NB2). Chromosomes are sorted based on high (dark-colored) and low (light-colored) subtelomeric TERRA transcription. Black dots denote m 6 A RIP-seq peaks identified using MACS peak caller in these NB tumor samples. ( J ) Genome browser screenshots showing m 6 A RIP/input ratio tracks for NB1 and NB2 tumor samples at two active chromosome ends (Chr12p, Chr20q) and one inactive chromosome end (Chr3p). Red bars mark m 6 A RIP peaks identified using MACS peak caller. ( K ) The m 6 A enrichment profiles across subtelomeres using T2T assembly in NB1 and NB2 tumors. The m 6 A RIP/input signals were normalized to spike-in and to the mappability likelihood of each chromosome ends. Subtelomeres (high and low TERRA transcription) were classified according to the normalized median expression shown. ( L ) Event-free survival of NB patients (n = 498, SEQC cohort) with either low (blue) or high (red) expression of METTL3. ( M ) Event-free survival of ALT+ NB patients (n = 21) with either low (blue) or high (red) expression of METTL3. ( N ) Bar graph showing the percentage of ALT+ or NMA tumors with copy number gain in METTL3 , METTL14 , or hnRNPA2B1 genes. ( O ) Immunohistochemistry (IHC) analysis of METTL3 and METTL14 in <t>human</t> <t>neuroblastoma</t> tumors belonging to either of the subgroups (ALT+, NMA- high risk, or low risk). Sections were counterstained with hematoxylin. One representative image is presented from each subgroup of NB patients.
    Imr 32, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology imr 32 cells
    Overexpression of KLF4 in human malignant neuroblastoma SK‐N‐DZ and <t>IMR‐32</t> cells. (A) Cells were transfected with control vector (pcDNA3.1/HisB) or KLF4 plasmid vector (pcDNA3.1/KLF4‐HisB). After transfection (24 h), ...
    Imr 32 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher imr 32 cells
    Effect of various toxic metal(loid)s on caspase activity and BIRC3 mRNA levels. Cell viability of <t>IMR-32</t> cells treated with methylmercury (CH HgCl) for indicated time ( a ). Cleaved caspase-3 expression ( b ) and the mRNA level of BIRC3 ( c ) of IMR-32 cells treated with methylmercury for 6 h. Cell viability of HK-cells treated with methylmercury for indicated time ( d ). Cleaved caspase-3 expression ( e ) and the mRNA level of BIRC3 ( f ) of HK-cells treated with methylmercury for 3 h. Cell viability of HK-2 cells treated with inorganic mercury (HgCl 2 ) for indicated time ( g ). Cleaved caspase-3 expression ( h ) and the mRNA level of BIRC3 ( i ) of HK-2 cells treated with inorganic mercury for 3 h. Cell viability of AML-12 cells treated with arsenic (NaAsO 2 ) for indicated time ( j ). Caspase-3 expression ( k ) and the mRNA level of Birc3 ( l ) of AML-12 cells treated with arsenic for 6 h. Cell viability of AML-12 cells treated with Cd for indicated time ( m ). Caspase-3 expression ( n ) and the mRNA level of Birc3 ( o ) of AML-12 cells treated with Cd for 6 h. ( a , d , g , j , m ) Cell viabilities were examined using MTT assays after the treatment with each toxic metal(loid) for the indicated times. Values are the means ± S.D. (n = 5). *Significantly different from the control group of 3 h treated group, P < 0.05. $ Significantly different from the control group of 6 h treated group, P < 0.05. # Significantly different from the control group of 24 h treated group, P < 0.05. The absence of an error bar indicates that the S.D. was within the area of the symbol. ( b , e , h , k , n ) Whole cell lysates were used for western blot analysis and probed with caspase-3 or cleaved caspase-3 antibody. GAPDH was probed as a loading control. The blots were run under the same experimental conditions and cropped from same membrane. Uncropped images are provided in Supplementary Fig. . ( c , f , i , l , o ) mRNA level of BIRC3 or Birc3 was examined using real-time RT-PCR. mRNA levels were normalized with GAPDH or β-actin . Values are the means ± S.D. (n = 3). * P < 0.05 vs. control.
    Imr 32 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore imr 32 cells
    Analysis of Notch ligands expression in neuroblastoma cells with different MYCN gene amplification. a RT-qPCR analysis of N-Myc mRNA expression levels in SH-SY5Y, KELLY and <t>IMR-32</t> neuroblastoma cell lines. b RT-qPCR analysis of the five mammalian Notch ligands in SH-SY5Y, KELLY and IMR-32 neuroblastoma cell lines. c Immunofluorescence analysis of DLL1 protein expression in SH-SY5Y and IMR-32 neuroblastoma cells. * p < 0.05 vs SH-SY5Y cell line
    Imr 32 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Microm International GmbH imr 32 cells
    Analysis of Notch ligands expression in neuroblastoma cells with different MYCN gene amplification. a RT-qPCR analysis of N-Myc mRNA expression levels in SH-SY5Y, KELLY and <t>IMR-32</t> neuroblastoma cell lines. b RT-qPCR analysis of the five mammalian Notch ligands in SH-SY5Y, KELLY and IMR-32 neuroblastoma cell lines. c Immunofluorescence analysis of DLL1 protein expression in SH-SY5Y and IMR-32 neuroblastoma cells. * p < 0.05 vs SH-SY5Y cell line
    Imr 32 Cells, supplied by Microm International GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    Image Search Results


    ( A ) Profiling TERRA expression in ALT+ and ALT- NB cell lines (with MYCN amplification [NMA] and without MYCN amplification [Non-NMA]) by slot blot assay performed with 50, 100, and 200 ng of RNA isolated from cell lines indicated. Blot probed with a DIG-labeled TERRA probe. TapeStation profile on the right shows 18s and 28s rRNA served as a loading control. Bar graph shows the TERRA level quantified from blots and normalized to 28s rRNA loading control. Data are shown as mean ± SD from three biological replicates. One-way ANOVA with Tukey's post hoc test was used, **** P < 0.0001. ( B ) m 6 A RIP followed by TERRA slot blot on RNA isolated from ALT+ and ALT-cell lines. RIP with IgG severed as a negative control. Bar graph shows the quantification of the blots. Data are shown as mean ± SD from three biological replicates. Sidak's multiple comparisons test was used, **** P < 0.0001; ns - nonsignificant P > 0.05. ( C ) TERRA foci (green) visualized by TERRA RNA-FISH in ALT+ (upper panel), NMA (middle panel) and Non-NMA (lower panel) NB cell lines. Box plot shows the quantification of the TERRA foci per nucleus. At least 67 cells were counted from three independent biological replicates. ( D ) TERRA foci (green) combined with m 6 A (red) IF in CHLA-90 (top) and SK-N-FI (bottom) cells. Box plot shows the number of overlaps of TERRA and m 6 A per nucleus. At least 65 cells were counted from three independent biological replicates. Scale bar is 10 μm. ( E ) The m 6 A enrichment profiles across all subtelomeres using T2T assembly in SK-N-FI and SK-N-BE cells. The m 6 A RIP/input signals were normalized to spike-in and to the mappability likelihood of each chromosome ends. ( F ) Box plot of spike-in, mappability-normalized m 6 A RIP/input enrichment profiles of all subtelomeres in SK-N-FI and SK-N-BE. Statistical significance was calculated using the Wilcoxon test, **** P < 0.0001. ( G , left panel) TERRA expression in ALT+, NMA and Non-NMA NB patient tumor RNA samples were detected by slot blot assay performed with 100 ng of total RNA. (Middle panel) TapeStation profile shows 18s and 28s rRNA served as a loading control, samples are loaded as NB1 to NB13 from top to bottom. (Right panel) Box plot shows the TERRA level quantified from blots and normalized to 28s rRNA loading control. Two-way ANOVA with Tukey's post hoc test was used, * P < 0.05; ns - nonsignificant P > 0.05. ( H ) Representative browser screenshot of 50 kb region around the telomere ends, showing TERRA RNA expression (CPM) from two of the active chromosome ends (Chr9p, Chr16q) and one inactive chromosome end (Chr3p) using Illumina short read RNA-seq performed on NB1 and NB2 tumor samples. CpG islands are marked with green bars and the telomeric repeats in this region are marked with black bars. ( I ) Heatmap summarizing RNA-seq and m 6 A RIP-seq data of two NB tumor samples (NB1 and NB2). Chromosomes are sorted based on high (dark-colored) and low (light-colored) subtelomeric TERRA transcription. Black dots denote m 6 A RIP-seq peaks identified using MACS peak caller in these NB tumor samples. ( J ) Genome browser screenshots showing m 6 A RIP/input ratio tracks for NB1 and NB2 tumor samples at two active chromosome ends (Chr12p, Chr20q) and one inactive chromosome end (Chr3p). Red bars mark m 6 A RIP peaks identified using MACS peak caller. ( K ) The m 6 A enrichment profiles across subtelomeres using T2T assembly in NB1 and NB2 tumors. The m 6 A RIP/input signals were normalized to spike-in and to the mappability likelihood of each chromosome ends. Subtelomeres (high and low TERRA transcription) were classified according to the normalized median expression shown. ( L ) Event-free survival of NB patients (n = 498, SEQC cohort) with either low (blue) or high (red) expression of METTL3. ( M ) Event-free survival of ALT+ NB patients (n = 21) with either low (blue) or high (red) expression of METTL3. ( N ) Bar graph showing the percentage of ALT+ or NMA tumors with copy number gain in METTL3 , METTL14 , or hnRNPA2B1 genes. ( O ) Immunohistochemistry (IHC) analysis of METTL3 and METTL14 in human neuroblastoma tumors belonging to either of the subgroups (ALT+, NMA- high risk, or low risk). Sections were counterstained with hematoxylin. One representative image is presented from each subgroup of NB patients.

    Journal: Nucleic Acids Research

    Article Title: METTL3 drives telomere targeting of TERRA lncRNA through m 6 A-dependent R-loop formation: a therapeutic target for ALT-positive neuroblastoma

    doi: 10.1093/nar/gkad1242

    Figure Lengend Snippet: ( A ) Profiling TERRA expression in ALT+ and ALT- NB cell lines (with MYCN amplification [NMA] and without MYCN amplification [Non-NMA]) by slot blot assay performed with 50, 100, and 200 ng of RNA isolated from cell lines indicated. Blot probed with a DIG-labeled TERRA probe. TapeStation profile on the right shows 18s and 28s rRNA served as a loading control. Bar graph shows the TERRA level quantified from blots and normalized to 28s rRNA loading control. Data are shown as mean ± SD from three biological replicates. One-way ANOVA with Tukey's post hoc test was used, **** P < 0.0001. ( B ) m 6 A RIP followed by TERRA slot blot on RNA isolated from ALT+ and ALT-cell lines. RIP with IgG severed as a negative control. Bar graph shows the quantification of the blots. Data are shown as mean ± SD from three biological replicates. Sidak's multiple comparisons test was used, **** P < 0.0001; ns - nonsignificant P > 0.05. ( C ) TERRA foci (green) visualized by TERRA RNA-FISH in ALT+ (upper panel), NMA (middle panel) and Non-NMA (lower panel) NB cell lines. Box plot shows the quantification of the TERRA foci per nucleus. At least 67 cells were counted from three independent biological replicates. ( D ) TERRA foci (green) combined with m 6 A (red) IF in CHLA-90 (top) and SK-N-FI (bottom) cells. Box plot shows the number of overlaps of TERRA and m 6 A per nucleus. At least 65 cells were counted from three independent biological replicates. Scale bar is 10 μm. ( E ) The m 6 A enrichment profiles across all subtelomeres using T2T assembly in SK-N-FI and SK-N-BE cells. The m 6 A RIP/input signals were normalized to spike-in and to the mappability likelihood of each chromosome ends. ( F ) Box plot of spike-in, mappability-normalized m 6 A RIP/input enrichment profiles of all subtelomeres in SK-N-FI and SK-N-BE. Statistical significance was calculated using the Wilcoxon test, **** P < 0.0001. ( G , left panel) TERRA expression in ALT+, NMA and Non-NMA NB patient tumor RNA samples were detected by slot blot assay performed with 100 ng of total RNA. (Middle panel) TapeStation profile shows 18s and 28s rRNA served as a loading control, samples are loaded as NB1 to NB13 from top to bottom. (Right panel) Box plot shows the TERRA level quantified from blots and normalized to 28s rRNA loading control. Two-way ANOVA with Tukey's post hoc test was used, * P < 0.05; ns - nonsignificant P > 0.05. ( H ) Representative browser screenshot of 50 kb region around the telomere ends, showing TERRA RNA expression (CPM) from two of the active chromosome ends (Chr9p, Chr16q) and one inactive chromosome end (Chr3p) using Illumina short read RNA-seq performed on NB1 and NB2 tumor samples. CpG islands are marked with green bars and the telomeric repeats in this region are marked with black bars. ( I ) Heatmap summarizing RNA-seq and m 6 A RIP-seq data of two NB tumor samples (NB1 and NB2). Chromosomes are sorted based on high (dark-colored) and low (light-colored) subtelomeric TERRA transcription. Black dots denote m 6 A RIP-seq peaks identified using MACS peak caller in these NB tumor samples. ( J ) Genome browser screenshots showing m 6 A RIP/input ratio tracks for NB1 and NB2 tumor samples at two active chromosome ends (Chr12p, Chr20q) and one inactive chromosome end (Chr3p). Red bars mark m 6 A RIP peaks identified using MACS peak caller. ( K ) The m 6 A enrichment profiles across subtelomeres using T2T assembly in NB1 and NB2 tumors. The m 6 A RIP/input signals were normalized to spike-in and to the mappability likelihood of each chromosome ends. Subtelomeres (high and low TERRA transcription) were classified according to the normalized median expression shown. ( L ) Event-free survival of NB patients (n = 498, SEQC cohort) with either low (blue) or high (red) expression of METTL3. ( M ) Event-free survival of ALT+ NB patients (n = 21) with either low (blue) or high (red) expression of METTL3. ( N ) Bar graph showing the percentage of ALT+ or NMA tumors with copy number gain in METTL3 , METTL14 , or hnRNPA2B1 genes. ( O ) Immunohistochemistry (IHC) analysis of METTL3 and METTL14 in human neuroblastoma tumors belonging to either of the subgroups (ALT+, NMA- high risk, or low risk). Sections were counterstained with hematoxylin. One representative image is presented from each subgroup of NB patients.

    Article Snippet: NB cell lines SK-N-FI (Sigma-Aldrich), SK-N-AS (ATCC), SH-SY5Y (CLS Cell Lines Service), CHLA-90 (CCOG), IMR-32 (CLS Cell Lines Service) and SK-N-BE(2) (DSMZ) cells were cultured in specific media.

    Techniques: Expressing, Amplification, Slot Blot Assay, Isolation, Labeling, Dot Blot, Negative Control, RNA Expression, RNA Sequencing Assay, Immunohistochemistry

    Overexpression of KLF4 in human malignant neuroblastoma SK‐N‐DZ and IMR‐32 cells. (A) Cells were transfected with control vector (pcDNA3.1/HisB) or KLF4 plasmid vector (pcDNA3.1/KLF4‐HisB). After transfection (24 h), ...

    Journal: Molecular oncology

    Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

    doi: 10.1016/j.molonc.2012.12.002

    Figure Lengend Snippet: Overexpression of KLF4 in human malignant neuroblastoma SK‐N‐DZ and IMR‐32 cells. (A) Cells were transfected with control vector (pcDNA3.1/HisB) or KLF4 plasmid vector (pcDNA3.1/KLF4‐HisB). After transfection (24 h), ...

    Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

    Techniques: Over Expression, Transfection, Plasmid Preparation

    Reduction in residual cell viability of SK‐N‐DZ and IMR‐32 cells after KLF4 overexpression and APG treatment. SK‐N‐DZ (A) and IMR‐32 (B) cells were transfected with various amount of KLF4 plasmid vector ...

    Journal: Molecular oncology

    Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

    doi: 10.1016/j.molonc.2012.12.002

    Figure Lengend Snippet: Reduction in residual cell viability of SK‐N‐DZ and IMR‐32 cells after KLF4 overexpression and APG treatment. SK‐N‐DZ (A) and IMR‐32 (B) cells were transfected with various amount of KLF4 plasmid vector ...

    Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

    Techniques: Over Expression, Transfection, Plasmid Preparation

    Morphological and biochemical detection of apoptosis in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM APG, 100 nM KLF4 vector + 25 μM ...

    Journal: Molecular oncology

    Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

    doi: 10.1016/j.molonc.2012.12.002

    Figure Lengend Snippet: Morphological and biochemical detection of apoptosis in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM APG, 100 nM KLF4 vector + 25 μM ...

    Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

    Techniques: Plasmid Preparation

    Regulation of expression of Bax and Bcl‐2 proteins in SK‐N‐DZ and IMR‐32 cells by KLF4. Treatments (24 h): control (CTL) cells, 100 nM Bcl‐2 shRNA, 100 nM KLF4 vector, and 100 nM ...

    Journal: Molecular oncology

    Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

    doi: 10.1016/j.molonc.2012.12.002

    Figure Lengend Snippet: Regulation of expression of Bax and Bcl‐2 proteins in SK‐N‐DZ and IMR‐32 cells by KLF4. Treatments (24 h): control (CTL) cells, 100 nM Bcl‐2 shRNA, 100 nM KLF4 vector, and 100 nM ...

    Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

    Techniques: Expressing, shRNA, Plasmid Preparation

    Regulation of Mcl‐1, Puma, Noxa, p53, caspase‐3, and ICAD by KLF4 and APG in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM ...

    Journal: Molecular oncology

    Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

    doi: 10.1016/j.molonc.2012.12.002

    Figure Lengend Snippet: Regulation of Mcl‐1, Puma, Noxa, p53, caspase‐3, and ICAD by KLF4 and APG in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM ...

    Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

    Techniques: Plasmid Preparation

    KLF4 overexpression and APG treatment reduced cell migration in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM APG, 100 nM KLF4 ...

    Journal: Molecular oncology

    Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

    doi: 10.1016/j.molonc.2012.12.002

    Figure Lengend Snippet: KLF4 overexpression and APG treatment reduced cell migration in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM APG, 100 nM KLF4 ...

    Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

    Techniques: Over Expression, Migration, Plasmid Preparation

    Effect of various toxic metal(loid)s on caspase activity and BIRC3 mRNA levels. Cell viability of IMR-32 cells treated with methylmercury (CH HgCl) for indicated time ( a ). Cleaved caspase-3 expression ( b ) and the mRNA level of BIRC3 ( c ) of IMR-32 cells treated with methylmercury for 6 h. Cell viability of HK-cells treated with methylmercury for indicated time ( d ). Cleaved caspase-3 expression ( e ) and the mRNA level of BIRC3 ( f ) of HK-cells treated with methylmercury for 3 h. Cell viability of HK-2 cells treated with inorganic mercury (HgCl 2 ) for indicated time ( g ). Cleaved caspase-3 expression ( h ) and the mRNA level of BIRC3 ( i ) of HK-2 cells treated with inorganic mercury for 3 h. Cell viability of AML-12 cells treated with arsenic (NaAsO 2 ) for indicated time ( j ). Caspase-3 expression ( k ) and the mRNA level of Birc3 ( l ) of AML-12 cells treated with arsenic for 6 h. Cell viability of AML-12 cells treated with Cd for indicated time ( m ). Caspase-3 expression ( n ) and the mRNA level of Birc3 ( o ) of AML-12 cells treated with Cd for 6 h. ( a , d , g , j , m ) Cell viabilities were examined using MTT assays after the treatment with each toxic metal(loid) for the indicated times. Values are the means ± S.D. (n = 5). *Significantly different from the control group of 3 h treated group, P < 0.05. $ Significantly different from the control group of 6 h treated group, P < 0.05. # Significantly different from the control group of 24 h treated group, P < 0.05. The absence of an error bar indicates that the S.D. was within the area of the symbol. ( b , e , h , k , n ) Whole cell lysates were used for western blot analysis and probed with caspase-3 or cleaved caspase-3 antibody. GAPDH was probed as a loading control. The blots were run under the same experimental conditions and cropped from same membrane. Uncropped images are provided in Supplementary Fig. . ( c , f , i , l , o ) mRNA level of BIRC3 or Birc3 was examined using real-time RT-PCR. mRNA levels were normalized with GAPDH or β-actin . Values are the means ± S.D. (n = 3). * P < 0.05 vs. control.

    Journal: Scientific Reports

    Article Title: Identification of ARNT-regulated BIRC3 as the target factor in cadmium renal toxicity

    doi: 10.1038/s41598-017-17494-9

    Figure Lengend Snippet: Effect of various toxic metal(loid)s on caspase activity and BIRC3 mRNA levels. Cell viability of IMR-32 cells treated with methylmercury (CH HgCl) for indicated time ( a ). Cleaved caspase-3 expression ( b ) and the mRNA level of BIRC3 ( c ) of IMR-32 cells treated with methylmercury for 6 h. Cell viability of HK-cells treated with methylmercury for indicated time ( d ). Cleaved caspase-3 expression ( e ) and the mRNA level of BIRC3 ( f ) of HK-cells treated with methylmercury for 3 h. Cell viability of HK-2 cells treated with inorganic mercury (HgCl 2 ) for indicated time ( g ). Cleaved caspase-3 expression ( h ) and the mRNA level of BIRC3 ( i ) of HK-2 cells treated with inorganic mercury for 3 h. Cell viability of AML-12 cells treated with arsenic (NaAsO 2 ) for indicated time ( j ). Caspase-3 expression ( k ) and the mRNA level of Birc3 ( l ) of AML-12 cells treated with arsenic for 6 h. Cell viability of AML-12 cells treated with Cd for indicated time ( m ). Caspase-3 expression ( n ) and the mRNA level of Birc3 ( o ) of AML-12 cells treated with Cd for 6 h. ( a , d , g , j , m ) Cell viabilities were examined using MTT assays after the treatment with each toxic metal(loid) for the indicated times. Values are the means ± S.D. (n = 5). *Significantly different from the control group of 3 h treated group, P < 0.05. $ Significantly different from the control group of 6 h treated group, P < 0.05. # Significantly different from the control group of 24 h treated group, P < 0.05. The absence of an error bar indicates that the S.D. was within the area of the symbol. ( b , e , h , k , n ) Whole cell lysates were used for western blot analysis and probed with caspase-3 or cleaved caspase-3 antibody. GAPDH was probed as a loading control. The blots were run under the same experimental conditions and cropped from same membrane. Uncropped images are provided in Supplementary Fig. . ( c , f , i , l , o ) mRNA level of BIRC3 or Birc3 was examined using real-time RT-PCR. mRNA levels were normalized with GAPDH or β-actin . Values are the means ± S.D. (n = 3). * P < 0.05 vs. control.

    Article Snippet: IMR-32 cells were grown in 96-well plates and cultured for 48 h. After treatment, 10% (w/v) Alamar Blue (Invitrogen, Grand Island, NY, USA) was added and incubated for 4 h at 37 °C.

    Techniques: Activity Assay, Expressing, Western Blot, Quantitative RT-PCR

    Analysis of Notch ligands expression in neuroblastoma cells with different MYCN gene amplification. a RT-qPCR analysis of N-Myc mRNA expression levels in SH-SY5Y, KELLY and IMR-32 neuroblastoma cell lines. b RT-qPCR analysis of the five mammalian Notch ligands in SH-SY5Y, KELLY and IMR-32 neuroblastoma cell lines. c Immunofluorescence analysis of DLL1 protein expression in SH-SY5Y and IMR-32 neuroblastoma cells. * p < 0.05 vs SH-SY5Y cell line

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: Analysis of Notch ligands expression in neuroblastoma cells with different MYCN gene amplification. a RT-qPCR analysis of N-Myc mRNA expression levels in SH-SY5Y, KELLY and IMR-32 neuroblastoma cell lines. b RT-qPCR analysis of the five mammalian Notch ligands in SH-SY5Y, KELLY and IMR-32 neuroblastoma cell lines. c Immunofluorescence analysis of DLL1 protein expression in SH-SY5Y and IMR-32 neuroblastoma cells. * p < 0.05 vs SH-SY5Y cell line

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Expressing, Amplification, Quantitative RT-PCR, Immunofluorescence

    Analysis of Notch pathway activation in neuroblastoma cells with different MYCN gene amplification. SH-SY5Y and IMR-32 cells were chosen as the two cell lines with the lowest and the highest N-Myc mRNA expression. a Confocal analysis of NICD expression levels in SH-SY5Y and IMR-32 cells. β III tubulin antibody ( green ) was used as neuron marker; NICD antibody ( red ) showed a typical nuclear localization, indicative of Notch activation. b Western blot analysis of NICD protein expression in SH-SY5Y and IMR-32 cells. c Densitometric analysis of NICD expression levels in SH-SY5Y and IMR-32 cells using Quantity One analysis software . * p < 0.05 vs SH-SY5Y cell line

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: Analysis of Notch pathway activation in neuroblastoma cells with different MYCN gene amplification. SH-SY5Y and IMR-32 cells were chosen as the two cell lines with the lowest and the highest N-Myc mRNA expression. a Confocal analysis of NICD expression levels in SH-SY5Y and IMR-32 cells. β III tubulin antibody ( green ) was used as neuron marker; NICD antibody ( red ) showed a typical nuclear localization, indicative of Notch activation. b Western blot analysis of NICD protein expression in SH-SY5Y and IMR-32 cells. c Densitometric analysis of NICD expression levels in SH-SY5Y and IMR-32 cells using Quantity One analysis software . * p < 0.05 vs SH-SY5Y cell line

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Activation Assay, Amplification, Expressing, Marker, Western Blot, Software

    Analysis of siRNA DLL1 and 13-cis retinoic acid treated IMR-32 neuroblastoma cell line. a Confocal analysis of siRNA DLL1 and 13-cis retinoic acid in IMR-32 neuroblastoma cells after 3 days of transfection. β III tubulin antibody was used as neuron marker. b Morphometric analysis of cell differentiation induced by siRNA DLL1 and 13-cis retinoic acid. Neuronal differentiation was evaluated by measuring neurite length. The percentage of differentiated cells was calculated, considered as cells with neurites ≥50 μM in length in IMR-32 cells. * p < 0.05 vs control

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: Analysis of siRNA DLL1 and 13-cis retinoic acid treated IMR-32 neuroblastoma cell line. a Confocal analysis of siRNA DLL1 and 13-cis retinoic acid in IMR-32 neuroblastoma cells after 3 days of transfection. β III tubulin antibody was used as neuron marker. b Morphometric analysis of cell differentiation induced by siRNA DLL1 and 13-cis retinoic acid. Neuronal differentiation was evaluated by measuring neurite length. The percentage of differentiated cells was calculated, considered as cells with neurites ≥50 μM in length in IMR-32 cells. * p < 0.05 vs control

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Transfection, Marker, Cell Differentiation

    RT-qPCR analysis of DLL1 mRNA expression levels in IMR-32 neuroblastoma cell line. Analysis of DLL1 mRNA expression levels after Authors’ contribution3 days of transfection with all miRNA-34 family members and with miRNA-210. * p < 0.05 vs control

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: RT-qPCR analysis of DLL1 mRNA expression levels in IMR-32 neuroblastoma cell line. Analysis of DLL1 mRNA expression levels after Authors’ contribution3 days of transfection with all miRNA-34 family members and with miRNA-210. * p < 0.05 vs control

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Quantitative RT-PCR, Expressing, Transfection

    Analysis of miRNA-34b transfection in IMR-32 neuroblastoma cells line. a Confocal analysis of miRNA-34b transfection in IMR-32 neuroblastoma cells after 3 days of transfection. β III tubulin antibody was used as neuron marker. b Morphometric analysis of cell differentiation induced by miRNAs transfection. IMR-32 neuroblastoma cells underwent 3 days of transfection and then neuronal differentiation was evaluated by measuring neurite length. The percentage of differentiated cells was calculated, considered as cells with neurites ≥50 μM in length in IMR-32 cells. * p < 0.05 vs control

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: Analysis of miRNA-34b transfection in IMR-32 neuroblastoma cells line. a Confocal analysis of miRNA-34b transfection in IMR-32 neuroblastoma cells after 3 days of transfection. β III tubulin antibody was used as neuron marker. b Morphometric analysis of cell differentiation induced by miRNAs transfection. IMR-32 neuroblastoma cells underwent 3 days of transfection and then neuronal differentiation was evaluated by measuring neurite length. The percentage of differentiated cells was calculated, considered as cells with neurites ≥50 μM in length in IMR-32 cells. * p < 0.05 vs control

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Transfection, Marker, Cell Differentiation

    Western Blot analysis of IMR-32 neuroblastoma cell line. Western Blot analysis of Neu N ( a ) and β III tubulin ( b ) protein expression levels after 3 days of miRNAs transfection. * p < 0.05 vs control

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: Western Blot analysis of IMR-32 neuroblastoma cell line. Western Blot analysis of Neu N ( a ) and β III tubulin ( b ) protein expression levels after 3 days of miRNAs transfection. * p < 0.05 vs control

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Western Blot, Expressing, Transfection

    Cell cycle analysis of IMR-32 neuroblastoma cell line. Cell cycle analysis, by Flow Cytometer, of IMR-32 cells after 3 days of miRNAs transfection. * p < 0.0001 vs G0/G1 phase control. ** p < 0.0001 vs S phase control

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: Cell cycle analysis of IMR-32 neuroblastoma cell line. Cell cycle analysis, by Flow Cytometer, of IMR-32 cells after 3 days of miRNAs transfection. * p < 0.0001 vs G0/G1 phase control. ** p < 0.0001 vs S phase control

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Cell Cycle Assay, Flow Cytometry, Transfection

    Apoptosis analysis of IMR-32 neuroblastoma cell line. a Annexin V/PI double staining and flow cytometry assays. X-axis indicates the number of Annexin V-FITC-stained cells. Y-axis indicates the number of PI-stained cells. b Determination of apoptosis percentages based on the accumulation of Annexin V-positive cells

    Journal: BMC Cancer

    Article Title: Notch ligand Delta-like 1 as a novel molecular target in childhood neuroblastoma

    doi: 10.1186/s12885-017-3340-3

    Figure Lengend Snippet: Apoptosis analysis of IMR-32 neuroblastoma cell line. a Annexin V/PI double staining and flow cytometry assays. X-axis indicates the number of Annexin V-FITC-stained cells. Y-axis indicates the number of PI-stained cells. b Determination of apoptosis percentages based on the accumulation of Annexin V-positive cells

    Article Snippet: SH-SY5Y cells were plated with a density of 75 × 10 3 /well in a 24 wells plate, grown on a glass coverslip (coated with poly-l-lysine, Sigma-Aldrich); IMR-32 cells were plated with a density of 50 × 10 3 /well and grown on a glass coverslip coated with collagen IV (BD Bioscience).

    Techniques: Double Staining, Flow Cytometry, Staining