immunostaining Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher immunohistochemical ihc analysis
    Immunohistochemical Ihc Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc analysis/product/Thermo Fisher
    Average 94 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc analysis - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    98
    Millipore immunohistochemical assays
    Effects of long-term exposure to Pb in Myelin basic protein (MBP) <t>immunostaining</t> (blue arrowhead) in cerebellum of Wistar rats. ( A ) Control group and ( B ) Lead group. The results are expressed as mean ± standard error of ( C ) area fraction percentage of immunostaining. * p
    Immunohistochemical Assays, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical assays/product/Millipore
    Average 98 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical assays - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    91
    Abcam immunohistochemical ihc
    Representive pictures of <t>immunohistochemical</t> staining of TPX2, IMP3, and PD-L1 in cervical tissues. Negative controls used non-immune isotype serum instead of primary antibodies. Arrows indicate the positively stained cells. Scale bars, 50 μm; original magnification, x200.
    Immunohistochemical Ihc, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc/product/Abcam
    Average 91 stars, based on 87 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    Cell Signaling Technology Inc immunohistochemical ihc analysis
    Examples of insulin receptor (IR) and IGF-1R <t>immunohistochemical</t> staining. Representative photomicrographs of IR and IGF-1R expression in lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC).
    Immunohistochemical Ihc Analysis, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc analysis/product/Cell Signaling Technology Inc
    Average 91 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc analysis - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    Agilent technologies immunohistochemical ihc assay
    Ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein 3 (ASB3) is generally down-regulated in colorectal cancer (CRC) tissues and cell lines. ASB3 expression was detected by <t>immunohistochemical</t> <t>(IHC),</t> real-time quantitative polymerase chain reaction (qPCR), and Western blotting assays in CRC tissues ( a , b , c , d and e ) and cell lines ( f and g ). a Representative images of IHC staining for ASB3 in CRC tissues. ASB3 protein expression levels were divided into 4 levels: I 0 , negative; I 1 , weak staining; I 2 , moderate staining; I 3 , strong staining. The scale bar represents 50μm. b ASB3 protein levels (H score) determined by IHC assay in 274 pairs of CRC and corresponding normal mucosal tissues (*** P
    Immunohistochemical Ihc Assay, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc assay/product/Agilent technologies
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc assay - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    88
    Ventana Medical nexes ihc immunostainer
    Ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein 3 (ASB3) is generally down-regulated in colorectal cancer (CRC) tissues and cell lines. ASB3 expression was detected by <t>immunohistochemical</t> <t>(IHC),</t> real-time quantitative polymerase chain reaction (qPCR), and Western blotting assays in CRC tissues ( a , b , c , d and e ) and cell lines ( f and g ). a Representative images of IHC staining for ASB3 in CRC tissues. ASB3 protein expression levels were divided into 4 levels: I 0 , negative; I 1 , weak staining; I 2 , moderate staining; I 3 , strong staining. The scale bar represents 50μm. b ASB3 protein levels (H score) determined by IHC assay in 274 pairs of CRC and corresponding normal mucosal tissues (*** P
    Nexes Ihc Immunostainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 88/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nexes ihc immunostainer/product/Ventana Medical
    Average 88 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    nexes ihc immunostainer - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    86
    Human Protein Atlas immunohistochemical ihc images
    Ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein 3 (ASB3) is generally down-regulated in colorectal cancer (CRC) tissues and cell lines. ASB3 expression was detected by <t>immunohistochemical</t> <t>(IHC),</t> real-time quantitative polymerase chain reaction (qPCR), and Western blotting assays in CRC tissues ( a , b , c , d and e ) and cell lines ( f and g ). a Representative images of IHC staining for ASB3 in CRC tissues. ASB3 protein expression levels were divided into 4 levels: I 0 , negative; I 1 , weak staining; I 2 , moderate staining; I 3 , strong staining. The scale bar represents 50μm. b ASB3 protein levels (H score) determined by IHC assay in 274 pairs of CRC and corresponding normal mucosal tissues (*** P
    Immunohistochemical Ihc Images, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc images/product/Human Protein Atlas
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc images - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    85
    Agilent technologies immunohistochemical ihc reagents
    Outcome of MSI and <t>IHC</t> analyses in three breast tumors from MLH1 mutation carriers . Left , MLH1 expressing, MSS breast carcinoma from individual 3:60 (Table 2). Middle , ductal carcinoma from the right breast from individual 77:15, displaying MLH1 protein loss and MSI. Right , lobular carcinoma from the left breast of the same patient, showing MLH1 protein loss and stable microsatellites. Red arrow in IHC stainings denotes lack of expression in tumor cells and green arrow positive expression in normal cells. MSI results are based on BAT25. IHC, immunohistory; MSI, microsatellite instability; MSS, microsatellite stable.
    Immunohistochemical Ihc Reagents, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc reagents/product/Agilent technologies
    Average 85 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc reagents - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    93
    Agilent technologies immunohistochemical ihc staining
    bMSC-derived Wnt5a promotes the maturation of HL60 cells. (A–C) The expression of CD13, CD14 and CD68 was evaluated by <t>immunohistochemical</t> staining assay following the culture of HL60 cells with conditional medium and the culture supernatants of bMSCs, adeno-vector bMSCs and adeno-Wnt5a bMSCs for 0, 24 and 48 h. (D–F) Immunohistochemical staining for CD13, CD14 and CD68 following the culture of HL60 cells with conditional medium and the culture supernatants containing Wnt5a neutralization antibodies against bMSCs, adeno-vector BMSCs and adeno-Wnt5a BMSCs for 0, 24 or 48 h. * P
    Immunohistochemical Ihc Staining, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc staining/product/Agilent technologies
    Average 93 stars, based on 409 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc staining - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    86
    Becton Dickinson immunohistochemical ihc zinc fixative
    Antiangiogenesis activity of lenvatinib in human thyroid cancer xenograft models in nude mice. Nude mice bearing tumor xenografts were treated orally once daily with either vehicle or lenvatinib at the indicated doses when tumor volumes reached between 100 and 300 mm 3 (day 1). Microvessel density (MVD) was analyzed by <t>immunohistochemical</t> staining of endothelial cells with an anti-mouse CD31 antibody within the resected tumor xenografts as described in Section 2 . MVD is expressed as the average number of microvessels per mm 2 in 5 regions of interest (ROIs). Each group consisted of 5 mice. Data are shown as means ± SD. * P
    Immunohistochemical Ihc Zinc Fixative, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc zinc fixative/product/Becton Dickinson
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc zinc fixative - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    92
    Biogenex immunohistochemical ihc staining
    <t>Immunohistochemical</t> analysis of the infiltration of T cells and macrophages, and the expression of IFN-γ, TNF-α, and IL-10 in OVA-challenged footpads. BALB/c mice were treated as the protocol shown in Figure 1 . Tissue sections of footpads were subjected to <t>IHC</t> staining for CD3, F4/80, IFN-γ, TNF-α, and IL-10. (A) Representative sections stained for CD3 are shown (original magnification, ×200). Cells with red signals around the blue nuclei indicate CD3 + cells. (B) Representative sections from the VH and CBD (10 mg/kg)-treated groups stained for F4/80, IFN-γ, TNF-α, and IL-10 are shown (original magnification, ×200). Quantitative data are shown in Table 1 . Results are representative of three independent experiments.
    Immunohistochemical Ihc Staining, supplied by Biogenex, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc staining/product/Biogenex
    Average 92 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc staining - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    88
    Ventana Medical immunohistochemi cal staining system
    <t>Immunohistochemical</t> analysis of the infiltration of T cells and macrophages, and the expression of IFN-γ, TNF-α, and IL-10 in OVA-challenged footpads. BALB/c mice were treated as the protocol shown in Figure 1 . Tissue sections of footpads were subjected to <t>IHC</t> staining for CD3, F4/80, IFN-γ, TNF-α, and IL-10. (A) Representative sections stained for CD3 are shown (original magnification, ×200). Cells with red signals around the blue nuclei indicate CD3 + cells. (B) Representative sections from the VH and CBD (10 mg/kg)-treated groups stained for F4/80, IFN-γ, TNF-α, and IL-10 are shown (original magnification, ×200). Quantitative data are shown in Table 1 . Results are representative of three independent experiments.
    Immunohistochemi Cal Staining System, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemi cal staining system/product/Ventana Medical
    Average 88 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    immunohistochemi cal staining system - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    90
    Human Protein Atlas immunohistochemical ihc staining
    Tumor-intrinsic programmed death 1 (PD-1) expression is widespread in many tumor types. (A) PD-1 mRNA expression level analysis in different cancer tissues from the human protein atlas. (B) PD-1 mRNA expression level analysis in various kinds of cancer cells based on related data from Cancer Cell Line Encyclopedia (CCLE). (C) . PD-1 protein expression level analysis in different cancer tissues using <t>immunohistochemical</t> staining from the human protein atlas. All cancer types were compared to “Central nervous system” tumors by t-test (P-values indicated). Tumors displaying highly significant differences ( P
    Immunohistochemical Ihc Staining, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc staining/product/Human Protein Atlas
    Average 90 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc staining - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    Lifespan Biosciences immunohistochemical ihc staining
    Tumor-intrinsic programmed death 1 (PD-1) expression is widespread in many tumor types. (A) PD-1 mRNA expression level analysis in different cancer tissues from the human protein atlas. (B) PD-1 mRNA expression level analysis in various kinds of cancer cells based on related data from Cancer Cell Line Encyclopedia (CCLE). (C) . PD-1 protein expression level analysis in different cancer tissues using <t>immunohistochemical</t> staining from the human protein atlas. All cancer types were compared to “Central nervous system” tumors by t-test (P-values indicated). Tumors displaying highly significant differences ( P
    Immunohistochemical Ihc Staining, supplied by Lifespan Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc staining/product/Lifespan Biosciences
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc staining - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Beijing CWBio ihc immunohistochemical detection kit
    Tumor-intrinsic programmed death 1 (PD-1) expression is widespread in many tumor types. (A) PD-1 mRNA expression level analysis in different cancer tissues from the human protein atlas. (B) PD-1 mRNA expression level analysis in various kinds of cancer cells based on related data from Cancer Cell Line Encyclopedia (CCLE). (C) . PD-1 protein expression level analysis in different cancer tissues using <t>immunohistochemical</t> staining from the human protein atlas. All cancer types were compared to “Central nervous system” tumors by t-test (P-values indicated). Tumors displaying highly significant differences ( P
    Ihc Immunohistochemical Detection Kit, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ihc immunohistochemical detection kit/product/Beijing CWBio
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    ihc immunohistochemical detection kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    85
    Ventana Medical nexes ihc automated immunostainer
    Tumor-intrinsic programmed death 1 (PD-1) expression is widespread in many tumor types. (A) PD-1 mRNA expression level analysis in different cancer tissues from the human protein atlas. (B) PD-1 mRNA expression level analysis in various kinds of cancer cells based on related data from Cancer Cell Line Encyclopedia (CCLE). (C) . PD-1 protein expression level analysis in different cancer tissues using <t>immunohistochemical</t> staining from the human protein atlas. All cancer types were compared to “Central nervous system” tumors by t-test (P-values indicated). Tumors displaying highly significant differences ( P
    Nexes Ihc Automated Immunostainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nexes ihc automated immunostainer/product/Ventana Medical
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    nexes ihc automated immunostainer - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    91
    Becton Dickinson immunohistochemical ihc
    Imaging analysis of the normal breast tissue in the tissue microarrays ( TMAs ). a The pipeline used in this analysis consisted of the three basic stages: TMA core detection and identification matching the identification grid with the core location ( upper panel ); region of interest (ROI) segmentation, machine-learning-based training and application of the epithelium/stroma classifier ( middle panel ); and cell and nuclei detection and staining evaluation ( bottom panel , left side ). Using this pipeline, data were readily available for downstream analysis ( bottom panel , right side - a detailed version of this panel is available in Additional file 1 : Figure S2). b Representative images of <t>immunohistochemical</t> <t>(IHC)</t> expression of EZH2 in normal breast tissues. Scale bar 200 μm
    Immunohistochemical Ihc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc/product/Becton Dickinson
    Average 91 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    Novocastra immunohistochemical ihc
    Imaging analysis of the normal breast tissue in the tissue microarrays ( TMAs ). a The pipeline used in this analysis consisted of the three basic stages: TMA core detection and identification matching the identification grid with the core location ( upper panel ); region of interest (ROI) segmentation, machine-learning-based training and application of the epithelium/stroma classifier ( middle panel ); and cell and nuclei detection and staining evaluation ( bottom panel , left side ). Using this pipeline, data were readily available for downstream analysis ( bottom panel , right side - a detailed version of this panel is available in Additional file 1 : Figure S2). b Representative images of <t>immunohistochemical</t> <t>(IHC)</t> expression of EZH2 in normal breast tissues. Scale bar 200 μm
    Immunohistochemical Ihc, supplied by Novocastra, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc/product/Novocastra
    Average 91 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    US Biomax immunohistochemical ihc
    Comparative analysis of IRF5 protein expression in human spleen tissue by <t>IHC</t> and IF microscopy. ( A ) Experimental details for the comparative analysis of α-IRF5 antibody specificity by IHC and IF microscopy. ( B ) Representative core images from IHC analysis of human spleen tissue. IRF5-positive staining is brown, hematoxylin (blue) stains cell nuclei; 2 nd alone, staining with secondary antibody only; RP, red pulp; WP, white pulp. ( C ) Same as ( B ) except tissue cores were double-stained with α-IRF5 antibodies (brown) and α-CD3 antibodies (red). ( D ) Representative images from IF analysis of α-IRF5 (red) and α-CD3 (green) co-staining in human spleen tissue. DAPI (blue) stains cell nuclei. Images were taken at 60X magnification.
    Immunohistochemical Ihc, supplied by US Biomax, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc/product/US Biomax
    Average 91 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    Ventana Medical immunohistochemical ihc
    PARP1 expression in human tongue tumors. ( a ) Surgically removed human squamous cell carcinoma specimens of the tongue were stained for PARP1 using an anti-PARP antibody and <t>immunohistochemical</t> detection. Adjacent sections were stained with H E for pathological evaluation and staging of the malignancy of the tissue. The dotted line marks the tumor margin in the displayed T2 staged tumor. The red squares indicate enlarged regions in adjacent images. The image shown is a representative example selected from n = 12 samples. ( b ) PARP1 quantification in human tongue tumor tissue in a waterfall plot of the PARP1-positive tissue area. The PARP1-positive tissue is represented by brown (PARP1) and the total tissue areas by blue staining. In each sample, 5–10 fields of view in the tumor area or adjacent healthy tissue were analyzed. Displayed are means ± SD. HTT = human tongue tumor; color code: orange = malignant tissue (squamous cell carcinoma T2-T4), dashed orange = premalignant cases (moderate to severe dysplasia, carcinoma in situ ), blue = corresponding normal adjacent tongue tissue. ( c ) PARP1-positive tissue area, grouped for normal tissue, premalignant, and malignant (T2-T4 tumor stages) cases. Statistical significance was determined using an analysis of variance where multiple comparisons were controlled using the Holm-Šidák at the familywise error rate of 5%. ( d ) Individual values for PARP1-positive tissue area, grouped for the pathologically assessed tumor stage (premalignant, T2, T3, T4) and compared to normal adjacent tissue. ( e ) Density plot of all PARP1-positive tissue area values from each field of view, pooled in two groups (normal and premalignant/malignant). See Supplementary Fig. S2 for additional statistical parameters.
    Immunohistochemical Ihc, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 91/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc/product/Ventana Medical
    Average 91 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    Zhongshan Golden Bridge Company immunohistochemical ihc
    Changes in gene and protein expression in condyle following MIA injection were evaluated by real-time PCR and <t>IHC,</t> respectively. A. Two weeks after MIA injection, anabolism-associated aggrecan and collagen I and II were downregulated compared with the control group. Catabolism-associated MMP3, MMP13, and ADAMTS5 were upregulated and TIMP2, but not TIMP1, was correspondingly downregulated. B. Two weeks after MIA (0.5 mg) injection, apoptosis-associated genes of the death receptor family, such as, TNFα, Fas, FasL, caspase8, caspase3, and BAX, but not caspase2 and caspase9, were significantly elevated in the MIA injection group; PCNA and α-SMA, representing proliferation and fibrous restoration, respectively, were upregulated (mean ± SEM; n = 6; ** P
    Immunohistochemical Ihc, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical ihc/product/Zhongshan Golden Bridge Company
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical ihc - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Effects of long-term exposure to Pb in Myelin basic protein (MBP) immunostaining (blue arrowhead) in cerebellum of Wistar rats. ( A ) Control group and ( B ) Lead group. The results are expressed as mean ± standard error of ( C ) area fraction percentage of immunostaining. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Long-Term Lead Exposure Since Adolescence Causes Proteomic and Morphological Alterations in the Cerebellum Associated with Motor Deficits in Adult Rats

    doi: 10.3390/ijms21103571

    Figure Lengend Snippet: Effects of long-term exposure to Pb in Myelin basic protein (MBP) immunostaining (blue arrowhead) in cerebellum of Wistar rats. ( A ) Control group and ( B ) Lead group. The results are expressed as mean ± standard error of ( C ) area fraction percentage of immunostaining. * p

    Article Snippet: Immunohistochemical Assays For immunohistochemical analyses, the slides with sections were dewaxed and rehydrated by heat, xylene and hydroalcoholic solutions and immersed in PBS for 3 min before incubation in citrate buffer at 70 °C for 25 min. After antigen retrieval, they were immersed in PBS for 10 min and immersed in methanol-hydrogen peroxide solution (3:100, v /v ) for endogenous peroxidase inhibition.

    Techniques: Immunostaining

    Effects of long-term exposure to Pb in synaptophysin (SYP) immunostaining (blue arrowhead) in cerebellum of Wistar rats. ( A ) Control group and ( B ) Lead group. The results are expressed as mean ± standard error of ( C ) area fraction percentage of immunostaining. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Long-Term Lead Exposure Since Adolescence Causes Proteomic and Morphological Alterations in the Cerebellum Associated with Motor Deficits in Adult Rats

    doi: 10.3390/ijms21103571

    Figure Lengend Snippet: Effects of long-term exposure to Pb in synaptophysin (SYP) immunostaining (blue arrowhead) in cerebellum of Wistar rats. ( A ) Control group and ( B ) Lead group. The results are expressed as mean ± standard error of ( C ) area fraction percentage of immunostaining. * p

    Article Snippet: Immunohistochemical Assays For immunohistochemical analyses, the slides with sections were dewaxed and rehydrated by heat, xylene and hydroalcoholic solutions and immersed in PBS for 3 min before incubation in citrate buffer at 70 °C for 25 min. After antigen retrieval, they were immersed in PBS for 10 min and immersed in methanol-hydrogen peroxide solution (3:100, v /v ) for endogenous peroxidase inhibition.

    Techniques: Immunostaining

    Representive pictures of immunohistochemical staining of TPX2, IMP3, and PD-L1 in cervical tissues. Negative controls used non-immune isotype serum instead of primary antibodies. Arrows indicate the positively stained cells. Scale bars, 50 μm; original magnification, x200.

    Journal: PLoS ONE

    Article Title: Positive Surgical Margin, HPV Persistence, and Expression of Both TPX2 and PD-L1 Are Associated with Persistence/Recurrence of Cervical Intraepithelial Neoplasia after Cervical Conization

    doi: 10.1371/journal.pone.0142868

    Figure Lengend Snippet: Representive pictures of immunohistochemical staining of TPX2, IMP3, and PD-L1 in cervical tissues. Negative controls used non-immune isotype serum instead of primary antibodies. Arrows indicate the positively stained cells. Scale bars, 50 μm; original magnification, x200.

    Article Snippet: Immunohistochemical (IHC) Staining IHC staining was performed using the following antibodies from Abcam (Cambridge, MA, USA): mouse anti-TPX2 (Ab32795, 1:300 dilution), rabbit anti-IMP3 (Ab109521, 1:100 dilution), and mouse anti-PDL1 (Ab58810, 1:40 dilution).

    Techniques: Immunohistochemistry, Staining

    Examples of insulin receptor (IR) and IGF-1R immunohistochemical staining. Representative photomicrographs of IR and IGF-1R expression in lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC).

    Journal: Cancer

    Article Title: Prognostic impact of Insulin Receptor Expression on Survival of Patients with Nonf-Small Cell Lung Cancer

    doi: 10.1002/cncr.26492

    Figure Lengend Snippet: Examples of insulin receptor (IR) and IGF-1R immunohistochemical staining. Representative photomicrographs of IR and IGF-1R expression in lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC).

    Article Snippet: Primary antibodies used in immunohistochemical (IHC) analysis were purchased from Cell Signaling Technology (IGF-1R) or Santa Cruz Biotechnology (IR).

    Techniques: Immunohistochemistry, Staining, Expressing

    Ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein 3 (ASB3) is generally down-regulated in colorectal cancer (CRC) tissues and cell lines. ASB3 expression was detected by immunohistochemical (IHC), real-time quantitative polymerase chain reaction (qPCR), and Western blotting assays in CRC tissues ( a , b , c , d and e ) and cell lines ( f and g ). a Representative images of IHC staining for ASB3 in CRC tissues. ASB3 protein expression levels were divided into 4 levels: I 0 , negative; I 1 , weak staining; I 2 , moderate staining; I 3 , strong staining. The scale bar represents 50μm. b ASB3 protein levels (H score) determined by IHC assay in 274 pairs of CRC and corresponding normal mucosal tissues (*** P

    Journal: Chinese Journal of Cancer

    Article Title: The loss-of-function mutations and down-regulated expression of ASB3 gene promote the growth and metastasis of colorectal cancer cells

    doi: 10.1186/s40880-017-0180-0

    Figure Lengend Snippet: Ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein 3 (ASB3) is generally down-regulated in colorectal cancer (CRC) tissues and cell lines. ASB3 expression was detected by immunohistochemical (IHC), real-time quantitative polymerase chain reaction (qPCR), and Western blotting assays in CRC tissues ( a , b , c , d and e ) and cell lines ( f and g ). a Representative images of IHC staining for ASB3 in CRC tissues. ASB3 protein expression levels were divided into 4 levels: I 0 , negative; I 1 , weak staining; I 2 , moderate staining; I 3 , strong staining. The scale bar represents 50μm. b ASB3 protein levels (H score) determined by IHC assay in 274 pairs of CRC and corresponding normal mucosal tissues (*** P

    Article Snippet: Immunohistochemical assay Protein levels of ASB3 were detected by immunohistochemical (IHC) assay with a peroxidase kit (DAKO, Carpinteria, CA, USA) as described previously [ , ].

    Techniques: Expressing, Immunohistochemistry, Real-time Polymerase Chain Reaction, Western Blot, Staining

    Immunohistochemical stain for melanoma tumor samples. PD-L1 chromogenic staining. a) Tumor with low PD-L1 expression and b) Tumor with high PD-L1 expression; Multiplexed IHC for the following markers: PD-L1, CD3, CD8, FoxP3, CD163 and Melanoma cocktail and DAPI as a counterstain; c) Short survival (PD): high CD8/CD163PDL1 and CD8/FoxP3 expression. d) Long survival (CR): low CD8/CD163PDL1 and CD8/FoxP3 expression;

    Journal: Oncoimmunology

    Article Title: PD-L1 expression with immune-infiltrate evaluation and outcome prediction in melanoma patients treated with ipilimumab

    doi: 10.1080/2162402X.2017.1405206

    Figure Lengend Snippet: Immunohistochemical stain for melanoma tumor samples. PD-L1 chromogenic staining. a) Tumor with low PD-L1 expression and b) Tumor with high PD-L1 expression; Multiplexed IHC for the following markers: PD-L1, CD3, CD8, FoxP3, CD163 and Melanoma cocktail and DAPI as a counterstain; c) Short survival (PD): high CD8/CD163PDL1 and CD8/FoxP3 expression. d) Long survival (CR): low CD8/CD163PDL1 and CD8/FoxP3 expression;

    Article Snippet: Baseline PD-L1 expression was measured by means of immunohistochemical (IHC) testing of FFPE tumor sections with the use of a rabbit monoclonal anti-human PD-L1 antibody (clone 28-8) and an automated assay developed by Dako.

    Techniques: Immunohistochemistry, Staining, Expressing

    Outcome of MSI and IHC analyses in three breast tumors from MLH1 mutation carriers . Left , MLH1 expressing, MSS breast carcinoma from individual 3:60 (Table 2). Middle , ductal carcinoma from the right breast from individual 77:15, displaying MLH1 protein loss and MSI. Right , lobular carcinoma from the left breast of the same patient, showing MLH1 protein loss and stable microsatellites. Red arrow in IHC stainings denotes lack of expression in tumor cells and green arrow positive expression in normal cells. MSI results are based on BAT25. IHC, immunohistory; MSI, microsatellite instability; MSS, microsatellite stable.

    Journal: Breast Cancer Research : BCR

    Article Title: Breast carcinoma and Lynch syndrome: molecular analysis of tumors arising in mutation carriers, non-carriers, and sporadic cases

    doi: 10.1186/bcr3205

    Figure Lengend Snippet: Outcome of MSI and IHC analyses in three breast tumors from MLH1 mutation carriers . Left , MLH1 expressing, MSS breast carcinoma from individual 3:60 (Table 2). Middle , ductal carcinoma from the right breast from individual 77:15, displaying MLH1 protein loss and MSI. Right , lobular carcinoma from the left breast of the same patient, showing MLH1 protein loss and stable microsatellites. Red arrow in IHC stainings denotes lack of expression in tumor cells and green arrow positive expression in normal cells. MSI results are based on BAT25. IHC, immunohistory; MSI, microsatellite instability; MSS, microsatellite stable.

    Article Snippet: The immunohistochemical (IHC) reagents (Dako EnVision+, Dako, Glostrup, Denmark/Carpinteria CA, USA) were utilized according to the manufacturer's specifications.

    Techniques: Immunohistochemistry, Mutagenesis, Expressing

    MMR status and TSG methylation in eight different tumor types from mutation carriers . A , for MMR status comparisons, the frequencies of MSI and abnormal IHC in the same tumors are indicated. B , Among TSGs, the top five loci affected by methylation in breast cancer were chosen for display. Dm > 0.25 was used as a cut-off for methylation for all tumors. The average number of methylated TSGs out of 24 per tumor is given below each tumor type. Dm, methylation dosage ratio; IHC, immunohistochemistry; MMR, mismatch repair; MSI, microsatellite instability; TSG, tumor suppressor gene.

    Journal: Breast Cancer Research : BCR

    Article Title: Breast carcinoma and Lynch syndrome: molecular analysis of tumors arising in mutation carriers, non-carriers, and sporadic cases

    doi: 10.1186/bcr3205

    Figure Lengend Snippet: MMR status and TSG methylation in eight different tumor types from mutation carriers . A , for MMR status comparisons, the frequencies of MSI and abnormal IHC in the same tumors are indicated. B , Among TSGs, the top five loci affected by methylation in breast cancer were chosen for display. Dm > 0.25 was used as a cut-off for methylation for all tumors. The average number of methylated TSGs out of 24 per tumor is given below each tumor type. Dm, methylation dosage ratio; IHC, immunohistochemistry; MMR, mismatch repair; MSI, microsatellite instability; TSG, tumor suppressor gene.

    Article Snippet: The immunohistochemical (IHC) reagents (Dako EnVision+, Dako, Glostrup, Denmark/Carpinteria CA, USA) were utilized according to the manufacturer's specifications.

    Techniques: Methylation, Mutagenesis, Immunohistochemistry

    bMSC-derived Wnt5a promotes the maturation of HL60 cells. (A–C) The expression of CD13, CD14 and CD68 was evaluated by immunohistochemical staining assay following the culture of HL60 cells with conditional medium and the culture supernatants of bMSCs, adeno-vector bMSCs and adeno-Wnt5a bMSCs for 0, 24 and 48 h. (D–F) Immunohistochemical staining for CD13, CD14 and CD68 following the culture of HL60 cells with conditional medium and the culture supernatants containing Wnt5a neutralization antibodies against bMSCs, adeno-vector BMSCs and adeno-Wnt5a BMSCs for 0, 24 or 48 h. * P

    Journal: Oncology Letters

    Article Title: Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non-canonical Wnt signaling pathway

    doi: 10.3892/ol.2014.2117

    Figure Lengend Snippet: bMSC-derived Wnt5a promotes the maturation of HL60 cells. (A–C) The expression of CD13, CD14 and CD68 was evaluated by immunohistochemical staining assay following the culture of HL60 cells with conditional medium and the culture supernatants of bMSCs, adeno-vector bMSCs and adeno-Wnt5a bMSCs for 0, 24 and 48 h. (D–F) Immunohistochemical staining for CD13, CD14 and CD68 following the culture of HL60 cells with conditional medium and the culture supernatants containing Wnt5a neutralization antibodies against bMSCs, adeno-vector BMSCs and adeno-Wnt5a BMSCs for 0, 24 or 48 h. * P

    Article Snippet: Immunohistochemical (IHC) staining For the IHC staining, the HL60 cells were seeded on microscope glass plates and non-specific staining was blocked using Dako Protein Block (Dako, Carpinteria, CA, USA) according to the manufacturer’s instructions.

    Techniques: Derivative Assay, Expressing, Immunohistochemistry, Staining, Plasmid Preparation, Neutralization

    Antiangiogenesis activity of lenvatinib in human thyroid cancer xenograft models in nude mice. Nude mice bearing tumor xenografts were treated orally once daily with either vehicle or lenvatinib at the indicated doses when tumor volumes reached between 100 and 300 mm 3 (day 1). Microvessel density (MVD) was analyzed by immunohistochemical staining of endothelial cells with an anti-mouse CD31 antibody within the resected tumor xenografts as described in Section 2 . MVD is expressed as the average number of microvessels per mm 2 in 5 regions of interest (ROIs). Each group consisted of 5 mice. Data are shown as means ± SD. * P

    Journal: Journal of Thyroid Research

    Article Title: Antitumor Activity of Lenvatinib (E7080): An Angiogenesis Inhibitor That Targets Multiple Receptor Tyrosine Kinases in Preclinical Human Thyroid Cancer Models

    doi: 10.1155/2014/638747

    Figure Lengend Snippet: Antiangiogenesis activity of lenvatinib in human thyroid cancer xenograft models in nude mice. Nude mice bearing tumor xenografts were treated orally once daily with either vehicle or lenvatinib at the indicated doses when tumor volumes reached between 100 and 300 mm 3 (day 1). Microvessel density (MVD) was analyzed by immunohistochemical staining of endothelial cells with an anti-mouse CD31 antibody within the resected tumor xenografts as described in Section 2 . MVD is expressed as the average number of microvessels per mm 2 in 5 regions of interest (ROIs). Each group consisted of 5 mice. Data are shown as means ± SD. * P

    Article Snippet: Immunohistochemical Analysis Tumor tissues were resected from nude mice, embedded in Optimal Cutting Temperature (OCT) compound (Sakura Finetek Japan, Tokyo, Japan), frozen in dry ice-acetone, and then sectioned (8 μ m), or they were embedded in paraffin, fixed overnight in immunohistochemical (IHC) Zinc Fixative (BD Biosciences), and then sectioned (6 μ m).

    Techniques: Activity Assay, Mouse Assay, Immunohistochemistry, Staining

    Immunohistochemical analysis of the infiltration of T cells and macrophages, and the expression of IFN-γ, TNF-α, and IL-10 in OVA-challenged footpads. BALB/c mice were treated as the protocol shown in Figure 1 . Tissue sections of footpads were subjected to IHC staining for CD3, F4/80, IFN-γ, TNF-α, and IL-10. (A) Representative sections stained for CD3 are shown (original magnification, ×200). Cells with red signals around the blue nuclei indicate CD3 + cells. (B) Representative sections from the VH and CBD (10 mg/kg)-treated groups stained for F4/80, IFN-γ, TNF-α, and IL-10 are shown (original magnification, ×200). Quantitative data are shown in Table 1 . Results are representative of three independent experiments.

    Journal: Acta Pharmacologica Sinica

    Article Title: Cannabidiol attenuates delayed-type hypersensitivity reactions via suppressing T-cell and macrophage reactivity

    doi: 10.1038/aps.2010.155

    Figure Lengend Snippet: Immunohistochemical analysis of the infiltration of T cells and macrophages, and the expression of IFN-γ, TNF-α, and IL-10 in OVA-challenged footpads. BALB/c mice were treated as the protocol shown in Figure 1 . Tissue sections of footpads were subjected to IHC staining for CD3, F4/80, IFN-γ, TNF-α, and IL-10. (A) Representative sections stained for CD3 are shown (original magnification, ×200). Cells with red signals around the blue nuclei indicate CD3 + cells. (B) Representative sections from the VH and CBD (10 mg/kg)-treated groups stained for F4/80, IFN-γ, TNF-α, and IL-10 are shown (original magnification, ×200). Quantitative data are shown in Table 1 . Results are representative of three independent experiments.

    Article Snippet: Reagents used for immunohistochemical (IHC) staining were purchased from BioGenex Laboratories (San Ramon, CA, USA) and AbCam, Inc (Cambridge, MA, USA).

    Techniques: Immunohistochemistry, Expressing, Mouse Assay, Staining

    Tumor-intrinsic programmed death 1 (PD-1) expression is widespread in many tumor types. (A) PD-1 mRNA expression level analysis in different cancer tissues from the human protein atlas. (B) PD-1 mRNA expression level analysis in various kinds of cancer cells based on related data from Cancer Cell Line Encyclopedia (CCLE). (C) . PD-1 protein expression level analysis in different cancer tissues using immunohistochemical staining from the human protein atlas. All cancer types were compared to “Central nervous system” tumors by t-test (P-values indicated). Tumors displaying highly significant differences ( P

    Journal: Frontiers in Immunology

    Article Title: Cancer Cell-Intrinsic PD-1 and Implications in Combinatorial Immunotherapy

    doi: 10.3389/fimmu.2018.01774

    Figure Lengend Snippet: Tumor-intrinsic programmed death 1 (PD-1) expression is widespread in many tumor types. (A) PD-1 mRNA expression level analysis in different cancer tissues from the human protein atlas. (B) PD-1 mRNA expression level analysis in various kinds of cancer cells based on related data from Cancer Cell Line Encyclopedia (CCLE). (C) . PD-1 protein expression level analysis in different cancer tissues using immunohistochemical staining from the human protein atlas. All cancer types were compared to “Central nervous system” tumors by t-test (P-values indicated). Tumors displaying highly significant differences ( P

    Article Snippet: To further analyze the PD-1 protein expression in different cancers, we also summarized the immunohistochemical (IHC) staining results in the Human Protein Atlas ( ).

    Techniques: Expressing, Immunohistochemistry, Staining

    Imaging analysis of the normal breast tissue in the tissue microarrays ( TMAs ). a The pipeline used in this analysis consisted of the three basic stages: TMA core detection and identification matching the identification grid with the core location ( upper panel ); region of interest (ROI) segmentation, machine-learning-based training and application of the epithelium/stroma classifier ( middle panel ); and cell and nuclei detection and staining evaluation ( bottom panel , left side ). Using this pipeline, data were readily available for downstream analysis ( bottom panel , right side - a detailed version of this panel is available in Additional file 1 : Figure S2). b Representative images of immunohistochemical (IHC) expression of EZH2 in normal breast tissues. Scale bar 200 μm

    Journal: Breast Cancer Research : BCR

    Article Title: EZH2 protein expression in normal breast epithelium and risk of breast cancer: results from the Nurses’ Health Studies

    doi: 10.1186/s13058-017-0817-6

    Figure Lengend Snippet: Imaging analysis of the normal breast tissue in the tissue microarrays ( TMAs ). a The pipeline used in this analysis consisted of the three basic stages: TMA core detection and identification matching the identification grid with the core location ( upper panel ); region of interest (ROI) segmentation, machine-learning-based training and application of the epithelium/stroma classifier ( middle panel ); and cell and nuclei detection and staining evaluation ( bottom panel , left side ). Using this pipeline, data were readily available for downstream analysis ( bottom panel , right side - a detailed version of this panel is available in Additional file 1 : Figure S2). b Representative images of immunohistochemical (IHC) expression of EZH2 in normal breast tissues. Scale bar 200 μm

    Article Snippet: For each immunohistochemical (IHC) stain, a 5-μm paraffin section was cut from each TMA block and immunostained for EZH2 (EZH2, 1:300, BD Biosciences, San Diego, CA, USA) after appropriate processing.

    Techniques: Imaging, Staining, Immunohistochemistry, Expressing

    Comparative analysis of IRF5 protein expression in human spleen tissue by IHC and IF microscopy. ( A ) Experimental details for the comparative analysis of α-IRF5 antibody specificity by IHC and IF microscopy. ( B ) Representative core images from IHC analysis of human spleen tissue. IRF5-positive staining is brown, hematoxylin (blue) stains cell nuclei; 2 nd alone, staining with secondary antibody only; RP, red pulp; WP, white pulp. ( C ) Same as ( B ) except tissue cores were double-stained with α-IRF5 antibodies (brown) and α-CD3 antibodies (red). ( D ) Representative images from IF analysis of α-IRF5 (red) and α-CD3 (green) co-staining in human spleen tissue. DAPI (blue) stains cell nuclei. Images were taken at 60X magnification.

    Journal: Scientific Reports

    Article Title: Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality

    doi: 10.1038/srep31002

    Figure Lengend Snippet: Comparative analysis of IRF5 protein expression in human spleen tissue by IHC and IF microscopy. ( A ) Experimental details for the comparative analysis of α-IRF5 antibody specificity by IHC and IF microscopy. ( B ) Representative core images from IHC analysis of human spleen tissue. IRF5-positive staining is brown, hematoxylin (blue) stains cell nuclei; 2 nd alone, staining with secondary antibody only; RP, red pulp; WP, white pulp. ( C ) Same as ( B ) except tissue cores were double-stained with α-IRF5 antibodies (brown) and α-CD3 antibodies (red). ( D ) Representative images from IF analysis of α-IRF5 (red) and α-CD3 (green) co-staining in human spleen tissue. DAPI (blue) stains cell nuclei. Images were taken at 60X magnification.

    Article Snippet: Immunohistochemical (IHC) and immunofluorescence (IF) microscopy Purchased human spleen arrays (US Biomax Inc: #sp241t, #sp481) were deparaffinized according to manufacturer’s instructions.

    Techniques: Expressing, Immunohistochemistry, Microscopy, Staining

    PARP1 expression in human tongue tumors. ( a ) Surgically removed human squamous cell carcinoma specimens of the tongue were stained for PARP1 using an anti-PARP antibody and immunohistochemical detection. Adjacent sections were stained with H E for pathological evaluation and staging of the malignancy of the tissue. The dotted line marks the tumor margin in the displayed T2 staged tumor. The red squares indicate enlarged regions in adjacent images. The image shown is a representative example selected from n = 12 samples. ( b ) PARP1 quantification in human tongue tumor tissue in a waterfall plot of the PARP1-positive tissue area. The PARP1-positive tissue is represented by brown (PARP1) and the total tissue areas by blue staining. In each sample, 5–10 fields of view in the tumor area or adjacent healthy tissue were analyzed. Displayed are means ± SD. HTT = human tongue tumor; color code: orange = malignant tissue (squamous cell carcinoma T2-T4), dashed orange = premalignant cases (moderate to severe dysplasia, carcinoma in situ ), blue = corresponding normal adjacent tongue tissue. ( c ) PARP1-positive tissue area, grouped for normal tissue, premalignant, and malignant (T2-T4 tumor stages) cases. Statistical significance was determined using an analysis of variance where multiple comparisons were controlled using the Holm-Šidák at the familywise error rate of 5%. ( d ) Individual values for PARP1-positive tissue area, grouped for the pathologically assessed tumor stage (premalignant, T2, T3, T4) and compared to normal adjacent tissue. ( e ) Density plot of all PARP1-positive tissue area values from each field of view, pooled in two groups (normal and premalignant/malignant). See Supplementary Fig. S2 for additional statistical parameters.

    Journal: Scientific Reports

    Article Title: Detection and delineation of oral cancer with a PARP1 targeted optical imaging agent

    doi: 10.1038/srep21371

    Figure Lengend Snippet: PARP1 expression in human tongue tumors. ( a ) Surgically removed human squamous cell carcinoma specimens of the tongue were stained for PARP1 using an anti-PARP antibody and immunohistochemical detection. Adjacent sections were stained with H E for pathological evaluation and staging of the malignancy of the tissue. The dotted line marks the tumor margin in the displayed T2 staged tumor. The red squares indicate enlarged regions in adjacent images. The image shown is a representative example selected from n = 12 samples. ( b ) PARP1 quantification in human tongue tumor tissue in a waterfall plot of the PARP1-positive tissue area. The PARP1-positive tissue is represented by brown (PARP1) and the total tissue areas by blue staining. In each sample, 5–10 fields of view in the tumor area or adjacent healthy tissue were analyzed. Displayed are means ± SD. HTT = human tongue tumor; color code: orange = malignant tissue (squamous cell carcinoma T2-T4), dashed orange = premalignant cases (moderate to severe dysplasia, carcinoma in situ ), blue = corresponding normal adjacent tongue tissue. ( c ) PARP1-positive tissue area, grouped for normal tissue, premalignant, and malignant (T2-T4 tumor stages) cases. Statistical significance was determined using an analysis of variance where multiple comparisons were controlled using the Holm-Šidák at the familywise error rate of 5%. ( d ) Individual values for PARP1-positive tissue area, grouped for the pathologically assessed tumor stage (premalignant, T2, T3, T4) and compared to normal adjacent tissue. ( e ) Density plot of all PARP1-positive tissue area values from each field of view, pooled in two groups (normal and premalignant/malignant). See Supplementary Fig. S2 for additional statistical parameters.

    Article Snippet: PARP1 expression in tissues PARP1 antigen in human oral cancer tissue, as well as FaDu and Cal27 xenografts and mouse tissues was detected using immunohistochemical (IHC) and immunofluorescence (IF) staining techniques, which were performed at the Molecular Cytology Core Facility of MSK using the Discovery XT processor (Ventana Medical Systems, Tucson, AZ).

    Techniques: Expressing, Staining, Immunohistochemistry, In Situ

    Changes in gene and protein expression in condyle following MIA injection were evaluated by real-time PCR and IHC, respectively. A. Two weeks after MIA injection, anabolism-associated aggrecan and collagen I and II were downregulated compared with the control group. Catabolism-associated MMP3, MMP13, and ADAMTS5 were upregulated and TIMP2, but not TIMP1, was correspondingly downregulated. B. Two weeks after MIA (0.5 mg) injection, apoptosis-associated genes of the death receptor family, such as, TNFα, Fas, FasL, caspase8, caspase3, and BAX, but not caspase2 and caspase9, were significantly elevated in the MIA injection group; PCNA and α-SMA, representing proliferation and fibrous restoration, respectively, were upregulated (mean ± SEM; n = 6; ** P

    Journal: PLoS ONE

    Article Title: Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate

    doi: 10.1371/journal.pone.0045036

    Figure Lengend Snippet: Changes in gene and protein expression in condyle following MIA injection were evaluated by real-time PCR and IHC, respectively. A. Two weeks after MIA injection, anabolism-associated aggrecan and collagen I and II were downregulated compared with the control group. Catabolism-associated MMP3, MMP13, and ADAMTS5 were upregulated and TIMP2, but not TIMP1, was correspondingly downregulated. B. Two weeks after MIA (0.5 mg) injection, apoptosis-associated genes of the death receptor family, such as, TNFα, Fas, FasL, caspase8, caspase3, and BAX, but not caspase2 and caspase9, were significantly elevated in the MIA injection group; PCNA and α-SMA, representing proliferation and fibrous restoration, respectively, were upregulated (mean ± SEM; n = 6; ** P

    Article Snippet: Immunohistochemical (IHC) Staining IHC staining was performed with a two-step detection kit (Zhongshan-Golden-Bridge-Biotechnology) as described previously .

    Techniques: Expressing, Injection, Real-time Polymerase Chain Reaction, Immunohistochemistry