immunosorbent assay protein concentrations Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher protein concentration total protein concentration
    PI3Kγ mediates innate immune memory cytokine response of microglia. Primary microglial cells (wild-type •, open columns; PI3Kγ −/− ■, dark gray columns; PI3Kγ KD/KD ▴, hatched columns) were primed with fixed priming doses of LPS [ULP (ultra-low dose, 1 fg/ml) and HP (high-dose, 100 ng/ml)] and restimulated on day 6 with a fixed dose LPS (100 ng/ml). Supernatants were collected 24 h after the second stimulation by LPS and the cytokine levels for (A) TNF-α, (B) IL-6, and (C) IL-10 were assayed by ELISA (normalized to <t>total</t> <t>protein</t> <t>concentration).</t> Data are shown as scatter dot plots, mean + SEM, n = 6, # p
    Protein Concentration Total Protein Concentration, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentration total protein concentration/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    protein concentration total protein concentration - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    96
    Millipore leptin concentrations
    PI3Kγ mediates innate immune memory cytokine response of microglia. Primary microglial cells (wild-type •, open columns; PI3Kγ −/− ■, dark gray columns; PI3Kγ KD/KD ▴, hatched columns) were primed with fixed priming doses of LPS [ULP (ultra-low dose, 1 fg/ml) and HP (high-dose, 100 ng/ml)] and restimulated on day 6 with a fixed dose LPS (100 ng/ml). Supernatants were collected 24 h after the second stimulation by LPS and the cytokine levels for (A) TNF-α, (B) IL-6, and (C) IL-10 were assayed by ELISA (normalized to <t>total</t> <t>protein</t> <t>concentration).</t> Data are shown as scatter dot plots, mean + SEM, n = 6, # p
    Leptin Concentrations, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptin concentrations/product/Millipore
    Average 96 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    leptin concentrations - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    90
    ALPCO leptin concentrations
    PI3Kγ mediates innate immune memory cytokine response of microglia. Primary microglial cells (wild-type •, open columns; PI3Kγ −/− ■, dark gray columns; PI3Kγ KD/KD ▴, hatched columns) were primed with fixed priming doses of LPS [ULP (ultra-low dose, 1 fg/ml) and HP (high-dose, 100 ng/ml)] and restimulated on day 6 with a fixed dose LPS (100 ng/ml). Supernatants were collected 24 h after the second stimulation by LPS and the cytokine levels for (A) TNF-α, (B) IL-6, and (C) IL-10 were assayed by ELISA (normalized to <t>total</t> <t>protein</t> <t>concentration).</t> Data are shown as scatter dot plots, mean + SEM, n = 6, # p
    Leptin Concentrations, supplied by ALPCO, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptin concentrations/product/ALPCO
    Average 90 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    leptin concentrations - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    91
    R&D Systems leptin concentrations
    PI3Kγ mediates innate immune memory cytokine response of microglia. Primary microglial cells (wild-type •, open columns; PI3Kγ −/− ■, dark gray columns; PI3Kγ KD/KD ▴, hatched columns) were primed with fixed priming doses of LPS [ULP (ultra-low dose, 1 fg/ml) and HP (high-dose, 100 ng/ml)] and restimulated on day 6 with a fixed dose LPS (100 ng/ml). Supernatants were collected 24 h after the second stimulation by LPS and the cytokine levels for (A) TNF-α, (B) IL-6, and (C) IL-10 were assayed by ELISA (normalized to <t>total</t> <t>protein</t> <t>concentration).</t> Data are shown as scatter dot plots, mean + SEM, n = 6, # p
    Leptin Concentrations, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptin concentrations/product/R&D Systems
    Average 91 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    leptin concentrations - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    94
    Millipore adiponectin concentrations
    Longitudinal changes in plasma <t>adiponectin</t> from third trimester of pregnancy to postpartum according to the mode of del ivery. Rio de Janeiro, 2009–2012.
    Adiponectin Concentrations, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adiponectin concentrations/product/Millipore
    Average 94 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    adiponectin concentrations - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher cytokine concentrations
    rIL-25 administration increased type 2 responses and suppressed inflammatory responses in E. histolytica -challenged mice. Cecal tissue was collected from rIL-25- or PBS-treated mice 7 days after E. histolytica challenge, and the cytokines IL-4, IL-5, IL-9, IL-17, IL-23, and TNF-α were measured by ELISA (A to C and G to I). <t>Cytokine</t> concentrations were normalized to total protein concentration. Inducible nitric oxide synthase ( Nos2 ), chitinase 3-like 1 ( Chi3l1 ), and eosinophil peroxidase ( Epx ) mRNAs were measured from cecal tissue of E. histolytica -challenged mice with or without rIL-25 treatment (D to F) after 1 day of E. histolytica challenge. *, P
    Cytokine Concentrations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine concentrations/product/Thermo Fisher
    Average 93 stars, based on 575 article reviews
    Price from $9.99 to $1999.99
    cytokine concentrations - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    90
    Merck KGaA serum adiponectin concentrations
    rIL-25 administration increased type 2 responses and suppressed inflammatory responses in E. histolytica -challenged mice. Cecal tissue was collected from rIL-25- or PBS-treated mice 7 days after E. histolytica challenge, and the cytokines IL-4, IL-5, IL-9, IL-17, IL-23, and TNF-α were measured by ELISA (A to C and G to I). <t>Cytokine</t> concentrations were normalized to total protein concentration. Inducible nitric oxide synthase ( Nos2 ), chitinase 3-like 1 ( Chi3l1 ), and eosinophil peroxidase ( Epx ) mRNAs were measured from cecal tissue of E. histolytica -challenged mice with or without rIL-25 treatment (D to F) after 1 day of E. histolytica challenge. *, P
    Serum Adiponectin Concentrations, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum adiponectin concentrations/product/Merck KGaA
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    serum adiponectin concentrations - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    bio-rad protein concentrations
    rIL-25 administration increased type 2 responses and suppressed inflammatory responses in E. histolytica -challenged mice. Cecal tissue was collected from rIL-25- or PBS-treated mice 7 days after E. histolytica challenge, and the cytokines IL-4, IL-5, IL-9, IL-17, IL-23, and TNF-α were measured by ELISA (A to C and G to I). <t>Cytokine</t> concentrations were normalized to total protein concentration. Inducible nitric oxide synthase ( Nos2 ), chitinase 3-like 1 ( Chi3l1 ), and eosinophil peroxidase ( Epx ) mRNAs were measured from cecal tissue of E. histolytica -challenged mice with or without rIL-25 treatment (D to F) after 1 day of E. histolytica challenge. *, P
    Protein Concentrations, supplied by bio-rad, used in various techniques. Bioz Stars score: 92/100, based on 6642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrations/product/bio-rad
    Average 92 stars, based on 6642 article reviews
    Price from $9.99 to $1999.99
    protein concentrations - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    R&D Systems il 17a concentrations
    A-B: Plasma IL-23 and <t>IL-17A</t> levels, corrected for plasma protein, in patients subjected to liver resection with or without I/R (N = 13 for control and N = 27 for I/R group). * p
    Il 17a Concentrations, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 17a concentrations/product/R&D Systems
    Average 94 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    il 17a concentrations - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    R&D Systems tnf α concentrations
    A-B: Plasma IL-23 and <t>IL-17A</t> levels, corrected for plasma protein, in patients subjected to liver resection with or without I/R (N = 13 for control and N = 27 for I/R group). * p
    Tnf α Concentrations, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α concentrations/product/R&D Systems
    Average 94 stars, based on 269 article reviews
    Price from $9.99 to $1999.99
    tnf α concentrations - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    90
    LINCO plasma adiponectin concentrations
    A-B: Plasma IL-23 and <t>IL-17A</t> levels, corrected for plasma protein, in patients subjected to liver resection with or without I/R (N = 13 for control and N = 27 for I/R group). * p
    Plasma Adiponectin Concentrations, supplied by LINCO, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma adiponectin concentrations/product/LINCO
    Average 90 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    plasma adiponectin concentrations - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher il 12 concentration
    A-B: Plasma IL-23 and <t>IL-17A</t> levels, corrected for plasma protein, in patients subjected to liver resection with or without I/R (N = 13 for control and N = 27 for I/R group). * p
    Il 12 Concentration, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 12 concentration/product/Thermo Fisher
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    il 12 concentration - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    R&D Systems interleukin 12 concentrations
    Interferon-γ production is increased in the livers of siRNA-treated HCC mice. INF-β, -γ, and <t>interleukin-12</t> levels in liver homogenates at 16 weeks were quantified using ELISA. Both NRP1 and control siRNAs significantly increase INF-γ levels in the livers of treated mice. ** P
    Interleukin 12 Concentrations, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/interleukin 12 concentrations/product/R&D Systems
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    interleukin 12 concentrations - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    90
    BioLegend cytokine concentrations
    Interferon-γ production is increased in the livers of siRNA-treated HCC mice. INF-β, -γ, and <t>interleukin-12</t> levels in liver homogenates at 16 weeks were quantified using ELISA. Both NRP1 and control siRNAs significantly increase INF-γ levels in the livers of treated mice. ** P
    Cytokine Concentrations, supplied by BioLegend, used in various techniques. Bioz Stars score: 90/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine concentrations/product/BioLegend
    Average 90 stars, based on 151 article reviews
    Price from $9.99 to $1999.99
    cytokine concentrations - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    91
    Crystal Chem Inc leptin concentrations
    Effects of FTIs on plasma insulin, <t>adiponectin</t> and <t>leptin</t> levels in mice treated with LPV/RTV. (A) No differences were observed in blood glucose levels between the groups. (B) LPV/RTV significantly increased plasma insulin concentrations compared with vehicle alone. Tipifarnib and lonafarnib significantly inhibited the LPV/RTV-induced increase in plasma insulin concentrations. Plasma insulin concentrations in the mice receiving the combination treatment of LPV/RTV and tipifarnib or LPV/RTV and lonafarnib did not significantly differ from those treated with vehicle alone, although plasma insulin concentrations appeared to be greater in the mice treated with LPV/RTV and FTI compared with vehicle alone. Treatment with LPV/RTV significantly decreased plasma (C) adiponectin and (D) leptin concentrations compared with vehicle alone. Tipifarnib and lonafarnib reversed the effects of LPV/RTV on adiponectin and leptin concentrations. *P
    Leptin Concentrations, supplied by Crystal Chem Inc, used in various techniques. Bioz Stars score: 91/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptin concentrations/product/Crystal Chem Inc
    Average 91 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    leptin concentrations - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    94
    Thermo Fisher il 17a concentrations
    Curcumin, but not carnosol, significantly inhibits T cell immune responses in ex-vivo stimulated PBMC from psoriasis patients. PBMC isolated from psoriasis patients (n = 9) were treated with carnosol (5 µM), curcumin (5 µM) or a vehicle control for 6 hours prior to stimulation with anti-CD3. After 4 days, supernatants were removed for analysis of IFNγ and IL-17 concentration by ELISA, and proliferation and cytokine production by T cells was analysed by flow cytometry. CD3 + CD8 - . ( A ) Pooled data depicting mean (±SEM) percentage expression of the proliferation marker Ki67 in CD3 + CD8 - T cells from control, carnosol or curcumin treated PBMC. ( B ) Pooled data depicting mean (±SEM) CD3 + TCRγδ + cells as a percentage of total live cells from control, carnosol or curcumin treated PBMC. ( C ) Pooled data depicting mean (±SEM) percentages of TNFα, IL-2, IFNγ, <t>IL-17A,</t> GM-CSF and IL-22 positive CD3 + CD8 − T cells from control, carnosol or curcumin treated PBMC. ( D ) Pooled data depicting mean (±SEM) concentrations of IFNγ and IL-17 in cell culture supernatants of control, carnosol and curcumin treated PBMC (means of three technical replicates per patient). ( E . ( F ) The cytokine expression profiles of T cells from control, carnosol and curcumin treated psoriasis PBMC were analysed using SPICE software. The pie charts represent the average frequencies of CD3 + CD8 - T cells producing every combination of cytokines for multiple patients (n = 9). The segments within the pie charts denote proportions of the CD3 + CD8 − T cell population producing different combinations of cytokines, and are heat-map coded to indicate increasing cytokine production. The arcs surrounding the pie charts indicate the cytokine(s) produced by that proportion of cells. Statistical significance was determined by one-way ANOVA with Dunnett’s post hoc test to compare carnosol and curcumin treatment to the control (**p
    Il 17a Concentrations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 17a concentrations/product/Thermo Fisher
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    il 17a concentrations - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    Shino-Test Corporation hmgb1 concentrations
    Effect of TCTP and the <t>HMGB1-TLR4/RAGE-NF-κB</t> pathway on the invasion potential of LoVo cells (magnification, ×400). The invasive capacity of LoVo NC cells (middle), LoVo TCTP-O cells (left) or LoVo-TCTP-K (right) were assessed by a cell invasion assay. The number of invasive cells was quantified by visual counting of six randomly selected fields. The groups comprised cells left untreated as controls, cells treated with rhHMGB1 (10 μ g/ml) or anti-HMGB1, anti-TLR4, anti-RAGE (all 5 μ g/ml) or the specific NF-κB inhibitor Bay117082 (5 μ mol/l), respectively. ** P
    Hmgb1 Concentrations, supplied by Shino-Test Corporation, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmgb1 concentrations/product/Shino-Test Corporation
    Average 93 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    hmgb1 concentrations - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    89
    Assay Designs Inc plasma leptin concentrations
    Effect of TCTP and the <t>HMGB1-TLR4/RAGE-NF-κB</t> pathway on the invasion potential of LoVo cells (magnification, ×400). The invasive capacity of LoVo NC cells (middle), LoVo TCTP-O cells (left) or LoVo-TCTP-K (right) were assessed by a cell invasion assay. The number of invasive cells was quantified by visual counting of six randomly selected fields. The groups comprised cells left untreated as controls, cells treated with rhHMGB1 (10 μ g/ml) or anti-HMGB1, anti-TLR4, anti-RAGE (all 5 μ g/ml) or the specific NF-κB inhibitor Bay117082 (5 μ mol/l), respectively. ** P
    Plasma Leptin Concentrations, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma leptin concentrations/product/Assay Designs Inc
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasma leptin concentrations - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    88
    Chondrex inc hmgb1 concentrations
    High-mobility group box 1 <t>(HMGB1)</t> mediates enhanced lung inflammatory responses following red blood cell (RBC) transfusion. ( A ) Bronchoalveolar lavage (BAL) KC and MIP-2 following transfusion alone, KC P = 0.71, MIP-2 P = 0.90. ( B ) BAL KC and MIP-2 following LPS injection, both * P
    Hmgb1 Concentrations, supplied by Chondrex inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmgb1 concentrations/product/Chondrex inc
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hmgb1 concentrations - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    Bio-Rad total protein measurements protein concentrations
    High-mobility group box 1 <t>(HMGB1)</t> mediates enhanced lung inflammatory responses following red blood cell (RBC) transfusion. ( A ) Bronchoalveolar lavage (BAL) KC and MIP-2 following transfusion alone, KC P = 0.71, MIP-2 P = 0.90. ( B ) BAL KC and MIP-2 following LPS injection, both * P
    Total Protein Measurements Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total protein measurements protein concentrations/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total protein measurements protein concentrations - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    TaKaRa lenti x concentrator
    High-mobility group box 1 <t>(HMGB1)</t> mediates enhanced lung inflammatory responses following red blood cell (RBC) transfusion. ( A ) Bronchoalveolar lavage (BAL) KC and MIP-2 following transfusion alone, KC P = 0.71, MIP-2 P = 0.90. ( B ) BAL KC and MIP-2 following LPS injection, both * P
    Lenti X Concentrator, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lenti x concentrator/product/TaKaRa
    Average 99 stars, based on 4792 article reviews
    Price from $9.99 to $1999.99
    lenti x concentrator - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher tumor necrosis factor α protein concentrations
    High-mobility group box 1 <t>(HMGB1)</t> mediates enhanced lung inflammatory responses following red blood cell (RBC) transfusion. ( A ) Bronchoalveolar lavage (BAL) KC and MIP-2 following transfusion alone, KC P = 0.71, MIP-2 P = 0.90. ( B ) BAL KC and MIP-2 following LPS injection, both * P
    Tumor Necrosis Factor α Protein Concentrations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tumor necrosis factor α protein concentrations/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tumor necrosis factor α protein concentrations - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    American Diagnostica fibronectin concentration
    High-mobility group box 1 <t>(HMGB1)</t> mediates enhanced lung inflammatory responses following red blood cell (RBC) transfusion. ( A ) Bronchoalveolar lavage (BAL) KC and MIP-2 following transfusion alone, KC P = 0.71, MIP-2 P = 0.90. ( B ) BAL KC and MIP-2 following LPS injection, both * P
    Fibronectin Concentration, supplied by American Diagnostica, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin concentration/product/American Diagnostica
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    fibronectin concentration - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    PI3Kγ mediates innate immune memory cytokine response of microglia. Primary microglial cells (wild-type •, open columns; PI3Kγ −/− ■, dark gray columns; PI3Kγ KD/KD ▴, hatched columns) were primed with fixed priming doses of LPS [ULP (ultra-low dose, 1 fg/ml) and HP (high-dose, 100 ng/ml)] and restimulated on day 6 with a fixed dose LPS (100 ng/ml). Supernatants were collected 24 h after the second stimulation by LPS and the cytokine levels for (A) TNF-α, (B) IL-6, and (C) IL-10 were assayed by ELISA (normalized to total protein concentration). Data are shown as scatter dot plots, mean + SEM, n = 6, # p

    Journal: Frontiers in Immunology

    Article Title: Memory-Like Inflammatory Responses of Microglia to Rising Doses of LPS: Key Role of PI3Kγ

    doi: 10.3389/fimmu.2019.02492

    Figure Lengend Snippet: PI3Kγ mediates innate immune memory cytokine response of microglia. Primary microglial cells (wild-type •, open columns; PI3Kγ −/− ■, dark gray columns; PI3Kγ KD/KD ▴, hatched columns) were primed with fixed priming doses of LPS [ULP (ultra-low dose, 1 fg/ml) and HP (high-dose, 100 ng/ml)] and restimulated on day 6 with a fixed dose LPS (100 ng/ml). Supernatants were collected 24 h after the second stimulation by LPS and the cytokine levels for (A) TNF-α, (B) IL-6, and (C) IL-10 were assayed by ELISA (normalized to total protein concentration). Data are shown as scatter dot plots, mean + SEM, n = 6, # p

    Article Snippet: Measurement of the Protein Concentration Total protein concentration was determined using the Pierce™ 660 nm Protein Assay Kit (#22662) from Thermo Fisher Scientific (Massachusetts, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

    Cytokine responses of microglia primed with increasing doses of LPS and β-glucan. (A) Schematic view of the two-step approach to induce memory-like inflammatory responses. Primary microglial cells (75,000 cells/well) isolated from neonatal mice were seeded on day 0 and stimulated with increasing doses of LPS (1 fg/ml to 100 ng/ml) or β-glucan (1 fg/ml to 1 μg/ml) on day 1 for 24 h. On day 2, cells were washed up and followed by medium change on day 4. Thereafter, cells were left to rest and restimulated on day 6 with a fixed dose of LPS (100 ng/ml). Six h and 24 h after the second stimulation, samples have been collected and processed for further analysis. (B–F) Opposing inflammatory response of microglia after priming with increasing doses of LPS or β-glucan. Microglial cells were stimulated with increasing doses of LPS ( B,C : 1 fg/ml to 100 ng/ml) or β-glucan ( D : 1 fg/ml to 1 μg/ml) for 24 h and rechallenged on day 6 with a fixed dose of LPS (100 ng/ml). Supernatants and protein lysates have been collected 24 h after the second stimulus by LPS (100 ng/ml). Cytokine levels ( B,D : TNF-α, n = 8; E : IL-6, n = 8; F : IL-10, n = 8) have been analyzed by ELISA (normalized to total protein concentration), whereas protein expression (C: TNF-α, n = 5) has been assayed by Western blotting and quantification (unprimed cells assigned as 1.0). Data are given as scatter dot plots, mean + SEM, # p

    Journal: Frontiers in Immunology

    Article Title: Memory-Like Inflammatory Responses of Microglia to Rising Doses of LPS: Key Role of PI3Kγ

    doi: 10.3389/fimmu.2019.02492

    Figure Lengend Snippet: Cytokine responses of microglia primed with increasing doses of LPS and β-glucan. (A) Schematic view of the two-step approach to induce memory-like inflammatory responses. Primary microglial cells (75,000 cells/well) isolated from neonatal mice were seeded on day 0 and stimulated with increasing doses of LPS (1 fg/ml to 100 ng/ml) or β-glucan (1 fg/ml to 1 μg/ml) on day 1 for 24 h. On day 2, cells were washed up and followed by medium change on day 4. Thereafter, cells were left to rest and restimulated on day 6 with a fixed dose of LPS (100 ng/ml). Six h and 24 h after the second stimulation, samples have been collected and processed for further analysis. (B–F) Opposing inflammatory response of microglia after priming with increasing doses of LPS or β-glucan. Microglial cells were stimulated with increasing doses of LPS ( B,C : 1 fg/ml to 100 ng/ml) or β-glucan ( D : 1 fg/ml to 1 μg/ml) for 24 h and rechallenged on day 6 with a fixed dose of LPS (100 ng/ml). Supernatants and protein lysates have been collected 24 h after the second stimulus by LPS (100 ng/ml). Cytokine levels ( B,D : TNF-α, n = 8; E : IL-6, n = 8; F : IL-10, n = 8) have been analyzed by ELISA (normalized to total protein concentration), whereas protein expression (C: TNF-α, n = 5) has been assayed by Western blotting and quantification (unprimed cells assigned as 1.0). Data are given as scatter dot plots, mean + SEM, # p

    Article Snippet: Measurement of the Protein Concentration Total protein concentration was determined using the Pierce™ 660 nm Protein Assay Kit (#22662) from Thermo Fisher Scientific (Massachusetts, USA).

    Techniques: Isolation, Mouse Assay, Enzyme-linked Immunosorbent Assay, Protein Concentration, Expressing, Western Blot

    Longitudinal changes in plasma adiponectin from third trimester of pregnancy to postpartum according to the mode of del ivery. Rio de Janeiro, 2009–2012.

    Journal: PLoS ONE

    Article Title: Changes in Maternal Plasma Adiponectin from Late Pregnancy to the Postpartum Period According to the Mode of Delivery: Results from a Prospective Cohort in Rio de Janeiro, Brazil

    doi: 10.1371/journal.pone.0158886

    Figure Lengend Snippet: Longitudinal changes in plasma adiponectin from third trimester of pregnancy to postpartum according to the mode of del ivery. Rio de Janeiro, 2009–2012.

    Article Snippet: Plasma adiponectin concentrations (μg/ml) were measured by enzyme linked immunosorbent assay (ELISA) using commercial kits (Millipore, St. Charles, Mo., USA) with a sensitivity of 0.78 ng/mL.

    Techniques:

    rIL-25 administration increased type 2 responses and suppressed inflammatory responses in E. histolytica -challenged mice. Cecal tissue was collected from rIL-25- or PBS-treated mice 7 days after E. histolytica challenge, and the cytokines IL-4, IL-5, IL-9, IL-17, IL-23, and TNF-α were measured by ELISA (A to C and G to I). Cytokine concentrations were normalized to total protein concentration. Inducible nitric oxide synthase ( Nos2 ), chitinase 3-like 1 ( Chi3l1 ), and eosinophil peroxidase ( Epx ) mRNAs were measured from cecal tissue of E. histolytica -challenged mice with or without rIL-25 treatment (D to F) after 1 day of E. histolytica challenge. *, P

    Journal: mBio

    Article Title: Role of Eosinophils and Tumor Necrosis Factor Alpha in Interleukin-25-Mediated Protection from Amebic Colitis

    doi: 10.1128/mBio.02329-16

    Figure Lengend Snippet: rIL-25 administration increased type 2 responses and suppressed inflammatory responses in E. histolytica -challenged mice. Cecal tissue was collected from rIL-25- or PBS-treated mice 7 days after E. histolytica challenge, and the cytokines IL-4, IL-5, IL-9, IL-17, IL-23, and TNF-α were measured by ELISA (A to C and G to I). Cytokine concentrations were normalized to total protein concentration. Inducible nitric oxide synthase ( Nos2 ), chitinase 3-like 1 ( Chi3l1 ), and eosinophil peroxidase ( Epx ) mRNAs were measured from cecal tissue of E. histolytica -challenged mice with or without rIL-25 treatment (D to F) after 1 day of E. histolytica challenge. *, P

    Article Snippet: Cytokine concentrations were normalized to total protein concentration (bicinchoninic acid [BCA] protein assay; Thermo Fisher).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Protein Concentration

    A-B: Plasma IL-23 and IL-17A levels, corrected for plasma protein, in patients subjected to liver resection with or without I/R (N = 13 for control and N = 27 for I/R group). * p

    Journal: Journal of Clinical and Translational Research

    Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man

    doi:

    Figure Lengend Snippet: A-B: Plasma IL-23 and IL-17A levels, corrected for plasma protein, in patients subjected to liver resection with or without I/R (N = 13 for control and N = 27 for I/R group). * p

    Article Snippet: Quantification of cytokines in murine plasma and liver tissue Murine plasma IL-23 and IL-17A concentrations were determined in heparin-anticoagulated plasma samples by ELISA according to the manufacturer’s instructions (Quantikine ELISA kits, R & D Systems).

    Techniques:

    A: ALT levels during reperfusion following different ischemia times (N = 6-8 per group). B: H E staining of livers subjected to 30-90 min of ischemia and 24 h of reperfusion. After 24 h, 20% necrosis was observed after 30 min ischemia (black arrows) versus 75-100% after 60-90 min ischemia. C-E: Liver IL-1β, IL-23, and RORγ mRNA expression following 30 or 60 min of ischemia and 6 or 24 h of reperfusion. Data are expressed as fold upregulation compared to the sham group (N = 3-5 per group). F-H: Plasma IL-23 levels determined by ELISA after 30, 60, or 90-min ischemia (N = 12 for sham and N = 4-9 for I/R groups). I-K: Plasma IL-17A protein levels after 30, 60, or 90 min of ischemia (N = 12 for sham and N = 4-9 for I/R groups). L: IL-17A protein levels after 60 min of ischemia in liver tissue homogenates (N = 8-10 per group). M: IL-17A protein levels after 90 min of ischemia in liver tissue homogenates (N = 6-9 per group). All data represent mean ± SEM.* p

    Journal: Journal of Clinical and Translational Research

    Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man

    doi:

    Figure Lengend Snippet: A: ALT levels during reperfusion following different ischemia times (N = 6-8 per group). B: H E staining of livers subjected to 30-90 min of ischemia and 24 h of reperfusion. After 24 h, 20% necrosis was observed after 30 min ischemia (black arrows) versus 75-100% after 60-90 min ischemia. C-E: Liver IL-1β, IL-23, and RORγ mRNA expression following 30 or 60 min of ischemia and 6 or 24 h of reperfusion. Data are expressed as fold upregulation compared to the sham group (N = 3-5 per group). F-H: Plasma IL-23 levels determined by ELISA after 30, 60, or 90-min ischemia (N = 12 for sham and N = 4-9 for I/R groups). I-K: Plasma IL-17A protein levels after 30, 60, or 90 min of ischemia (N = 12 for sham and N = 4-9 for I/R groups). L: IL-17A protein levels after 60 min of ischemia in liver tissue homogenates (N = 8-10 per group). M: IL-17A protein levels after 90 min of ischemia in liver tissue homogenates (N = 6-9 per group). All data represent mean ± SEM.* p

    Article Snippet: Quantification of cytokines in murine plasma and liver tissue Murine plasma IL-23 and IL-17A concentrations were determined in heparin-anticoagulated plasma samples by ELISA according to the manufacturer’s instructions (Quantikine ELISA kits, R & D Systems).

    Techniques: Staining, Expressing, Enzyme-linked Immunosorbent Assay

    Interferon-γ production is increased in the livers of siRNA-treated HCC mice. INF-β, -γ, and interleukin-12 levels in liver homogenates at 16 weeks were quantified using ELISA. Both NRP1 and control siRNAs significantly increase INF-γ levels in the livers of treated mice. ** P

    Journal: The American Journal of Pathology

    Article Title: Small Interfering RNAs Induce Target-Independent Inhibition of Tumor Growth and Vasculature Remodeling in a Mouse Model of Hepatocellular Carcinoma

    doi: 10.2353/ajpath.2010.100157

    Figure Lengend Snippet: Interferon-γ production is increased in the livers of siRNA-treated HCC mice. INF-β, -γ, and interleukin-12 levels in liver homogenates at 16 weeks were quantified using ELISA. Both NRP1 and control siRNAs significantly increase INF-γ levels in the livers of treated mice. ** P

    Article Snippet: Interferon-α, -β, and interleukin-12 concentrations in liver homogenates were estimated using the Mouse Interferon α ELISA Kit, Mouse Interferon β ELISA Kit, and Quantikine Mouse IL-12 p70 Immunoassay (R & D Systems), respectively, according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Effects of FTIs on plasma insulin, adiponectin and leptin levels in mice treated with LPV/RTV. (A) No differences were observed in blood glucose levels between the groups. (B) LPV/RTV significantly increased plasma insulin concentrations compared with vehicle alone. Tipifarnib and lonafarnib significantly inhibited the LPV/RTV-induced increase in plasma insulin concentrations. Plasma insulin concentrations in the mice receiving the combination treatment of LPV/RTV and tipifarnib or LPV/RTV and lonafarnib did not significantly differ from those treated with vehicle alone, although plasma insulin concentrations appeared to be greater in the mice treated with LPV/RTV and FTI compared with vehicle alone. Treatment with LPV/RTV significantly decreased plasma (C) adiponectin and (D) leptin concentrations compared with vehicle alone. Tipifarnib and lonafarnib reversed the effects of LPV/RTV on adiponectin and leptin concentrations. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Farnesyltransferase inhibitors prevent HIV protease inhibitor (lopinavir/ritonavir)-induced lipodystrophy and metabolic syndrome in mice

    doi: 10.3892/etm.2017.5526

    Figure Lengend Snippet: Effects of FTIs on plasma insulin, adiponectin and leptin levels in mice treated with LPV/RTV. (A) No differences were observed in blood glucose levels between the groups. (B) LPV/RTV significantly increased plasma insulin concentrations compared with vehicle alone. Tipifarnib and lonafarnib significantly inhibited the LPV/RTV-induced increase in plasma insulin concentrations. Plasma insulin concentrations in the mice receiving the combination treatment of LPV/RTV and tipifarnib or LPV/RTV and lonafarnib did not significantly differ from those treated with vehicle alone, although plasma insulin concentrations appeared to be greater in the mice treated with LPV/RTV and FTI compared with vehicle alone. Treatment with LPV/RTV significantly decreased plasma (C) adiponectin and (D) leptin concentrations compared with vehicle alone. Tipifarnib and lonafarnib reversed the effects of LPV/RTV on adiponectin and leptin concentrations. *P

    Article Snippet: Insulin, adiponectin and leptin concentrations in plasma were evaluated by ELISA according to the manufacturer's assay protocols of commercially available kits [an Ultra Sensitive Mouse Insulin ELISA kit (cat no. 90080) for insulin, a Mouse Adiponectin ELISA kit (cat no. 80569) for adiponectin and a Mouse Leptin ELISA kit (cat no. 90030) for leptin (Crystal Chem, Inc., Downers Grove, IL, USA)].

    Techniques: Mouse Assay

    Curcumin, but not carnosol, significantly inhibits T cell immune responses in ex-vivo stimulated PBMC from psoriasis patients. PBMC isolated from psoriasis patients (n = 9) were treated with carnosol (5 µM), curcumin (5 µM) or a vehicle control for 6 hours prior to stimulation with anti-CD3. After 4 days, supernatants were removed for analysis of IFNγ and IL-17 concentration by ELISA, and proliferation and cytokine production by T cells was analysed by flow cytometry. CD3 + CD8 - . ( A ) Pooled data depicting mean (±SEM) percentage expression of the proliferation marker Ki67 in CD3 + CD8 - T cells from control, carnosol or curcumin treated PBMC. ( B ) Pooled data depicting mean (±SEM) CD3 + TCRγδ + cells as a percentage of total live cells from control, carnosol or curcumin treated PBMC. ( C ) Pooled data depicting mean (±SEM) percentages of TNFα, IL-2, IFNγ, IL-17A, GM-CSF and IL-22 positive CD3 + CD8 − T cells from control, carnosol or curcumin treated PBMC. ( D ) Pooled data depicting mean (±SEM) concentrations of IFNγ and IL-17 in cell culture supernatants of control, carnosol and curcumin treated PBMC (means of three technical replicates per patient). ( E . ( F ) The cytokine expression profiles of T cells from control, carnosol and curcumin treated psoriasis PBMC were analysed using SPICE software. The pie charts represent the average frequencies of CD3 + CD8 - T cells producing every combination of cytokines for multiple patients (n = 9). The segments within the pie charts denote proportions of the CD3 + CD8 − T cell population producing different combinations of cytokines, and are heat-map coded to indicate increasing cytokine production. The arcs surrounding the pie charts indicate the cytokine(s) produced by that proportion of cells. Statistical significance was determined by one-way ANOVA with Dunnett’s post hoc test to compare carnosol and curcumin treatment to the control (**p

    Journal: Scientific Reports

    Article Title: Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment

    doi: 10.1038/s41598-018-28488-6

    Figure Lengend Snippet: Curcumin, but not carnosol, significantly inhibits T cell immune responses in ex-vivo stimulated PBMC from psoriasis patients. PBMC isolated from psoriasis patients (n = 9) were treated with carnosol (5 µM), curcumin (5 µM) or a vehicle control for 6 hours prior to stimulation with anti-CD3. After 4 days, supernatants were removed for analysis of IFNγ and IL-17 concentration by ELISA, and proliferation and cytokine production by T cells was analysed by flow cytometry. CD3 + CD8 - . ( A ) Pooled data depicting mean (±SEM) percentage expression of the proliferation marker Ki67 in CD3 + CD8 - T cells from control, carnosol or curcumin treated PBMC. ( B ) Pooled data depicting mean (±SEM) CD3 + TCRγδ + cells as a percentage of total live cells from control, carnosol or curcumin treated PBMC. ( C ) Pooled data depicting mean (±SEM) percentages of TNFα, IL-2, IFNγ, IL-17A, GM-CSF and IL-22 positive CD3 + CD8 − T cells from control, carnosol or curcumin treated PBMC. ( D ) Pooled data depicting mean (±SEM) concentrations of IFNγ and IL-17 in cell culture supernatants of control, carnosol and curcumin treated PBMC (means of three technical replicates per patient). ( E . ( F ) The cytokine expression profiles of T cells from control, carnosol and curcumin treated psoriasis PBMC were analysed using SPICE software. The pie charts represent the average frequencies of CD3 + CD8 - T cells producing every combination of cytokines for multiple patients (n = 9). The segments within the pie charts denote proportions of the CD3 + CD8 − T cell population producing different combinations of cytokines, and are heat-map coded to indicate increasing cytokine production. The arcs surrounding the pie charts indicate the cytokine(s) produced by that proportion of cells. Statistical significance was determined by one-way ANOVA with Dunnett’s post hoc test to compare carnosol and curcumin treatment to the control (**p

    Article Snippet: After 4 days supernatants were removed for analysis of IFNγ and IL-17A concentrations by ELISA (Ready-Set-Go kit; eBioscience), and PBMC were restimulated for flow cytometry.

    Techniques: Ex Vivo, Isolation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry, Expressing, Marker, Cell Culture, Software, Produced

    Effect of TCTP and the HMGB1-TLR4/RAGE-NF-κB pathway on the invasion potential of LoVo cells (magnification, ×400). The invasive capacity of LoVo NC cells (middle), LoVo TCTP-O cells (left) or LoVo-TCTP-K (right) were assessed by a cell invasion assay. The number of invasive cells was quantified by visual counting of six randomly selected fields. The groups comprised cells left untreated as controls, cells treated with rhHMGB1 (10 μ g/ml) or anti-HMGB1, anti-TLR4, anti-RAGE (all 5 μ g/ml) or the specific NF-κB inhibitor Bay117082 (5 μ mol/l), respectively. ** P

    Journal: International Journal of Oncology

    Article Title: Translationally controlled tumor protein affects colorectal cancer metastasis through the high mobility group box 1-dependent pathway

    doi: 10.3892/ijo.2018.4502

    Figure Lengend Snippet: Effect of TCTP and the HMGB1-TLR4/RAGE-NF-κB pathway on the invasion potential of LoVo cells (magnification, ×400). The invasive capacity of LoVo NC cells (middle), LoVo TCTP-O cells (left) or LoVo-TCTP-K (right) were assessed by a cell invasion assay. The number of invasive cells was quantified by visual counting of six randomly selected fields. The groups comprised cells left untreated as controls, cells treated with rhHMGB1 (10 μ g/ml) or anti-HMGB1, anti-TLR4, anti-RAGE (all 5 μ g/ml) or the specific NF-κB inhibitor Bay117082 (5 μ mol/l), respectively. ** P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The HMGB1 concentrations in serum samples were quantified by means of an HMGB1 ELISA Kit II (Shino-Test, Tokyo, Japan) according to the manufacturer's protocol.

    Techniques: Invasion Assay

    Effect of antibodies against HMGB1 or its receptors TLR4 and RAGE on the TCTP-induced activation of NF-κB in LoVo cells. (A) Immunoblotting analysis of the expression of cytoplasmic IκBα and nuclear NF-κB p65. Lanes: 1, LoVo-NC; 2, TCTP-O; 3, TCTP-K; 4, TCTP-O +10 μ g/ml rhHMGB1; 5, TCTP-O + 5 μ g/ml anti-HMGB1; 6, TCTP-O + 5 μ g/ml anti-TLR4; 7, TCTP-O + 5 μ g/ml anti-RAGE. GAPDH and Lamin B were used as internal controls of cytoplasmic and nuclear protein, respectively. * P

    Journal: International Journal of Oncology

    Article Title: Translationally controlled tumor protein affects colorectal cancer metastasis through the high mobility group box 1-dependent pathway

    doi: 10.3892/ijo.2018.4502

    Figure Lengend Snippet: Effect of antibodies against HMGB1 or its receptors TLR4 and RAGE on the TCTP-induced activation of NF-κB in LoVo cells. (A) Immunoblotting analysis of the expression of cytoplasmic IκBα and nuclear NF-κB p65. Lanes: 1, LoVo-NC; 2, TCTP-O; 3, TCTP-K; 4, TCTP-O +10 μ g/ml rhHMGB1; 5, TCTP-O + 5 μ g/ml anti-HMGB1; 6, TCTP-O + 5 μ g/ml anti-TLR4; 7, TCTP-O + 5 μ g/ml anti-RAGE. GAPDH and Lamin B were used as internal controls of cytoplasmic and nuclear protein, respectively. * P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The HMGB1 concentrations in serum samples were quantified by means of an HMGB1 ELISA Kit II (Shino-Test, Tokyo, Japan) according to the manufacturer's protocol.

    Techniques: Activation Assay, Expressing

    Detection of the histological expression of TCTP and serum value of HMGB1 in patients with CRC. (A) Immunohistochemical detection of expression of TCTP in CRC tissues (upper panel) and metastatic lymph nodes (lower panel) and the adjacent normal tissues. Specific TCTP staining is shown in brown. Panels a, c, d and f, show a magnification of ×400; panels b and e show a magnification of ×100. Panels a, c, d and f are amplifications of the marked (with squares) areas shown in panels b and e, respectively ( *** P

    Journal: International Journal of Oncology

    Article Title: Translationally controlled tumor protein affects colorectal cancer metastasis through the high mobility group box 1-dependent pathway

    doi: 10.3892/ijo.2018.4502

    Figure Lengend Snippet: Detection of the histological expression of TCTP and serum value of HMGB1 in patients with CRC. (A) Immunohistochemical detection of expression of TCTP in CRC tissues (upper panel) and metastatic lymph nodes (lower panel) and the adjacent normal tissues. Specific TCTP staining is shown in brown. Panels a, c, d and f, show a magnification of ×400; panels b and e show a magnification of ×100. Panels a, c, d and f are amplifications of the marked (with squares) areas shown in panels b and e, respectively ( *** P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The HMGB1 concentrations in serum samples were quantified by means of an HMGB1 ELISA Kit II (Shino-Test, Tokyo, Japan) according to the manufacturer's protocol.

    Techniques: Expressing, Immunohistochemistry, Staining

    Effect of TCTP on the expression of HMGB1 in xenograft tumors in nude mice. (A) Expression of TCTP and HMGB1 in the developed tumors in the nude mouse spleen were detected 5 weeks following inoculation with LoVo cells (magnification, ×400). * P

    Journal: International Journal of Oncology

    Article Title: Translationally controlled tumor protein affects colorectal cancer metastasis through the high mobility group box 1-dependent pathway

    doi: 10.3892/ijo.2018.4502

    Figure Lengend Snippet: Effect of TCTP on the expression of HMGB1 in xenograft tumors in nude mice. (A) Expression of TCTP and HMGB1 in the developed tumors in the nude mouse spleen were detected 5 weeks following inoculation with LoVo cells (magnification, ×400). * P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The HMGB1 concentrations in serum samples were quantified by means of an HMGB1 ELISA Kit II (Shino-Test, Tokyo, Japan) according to the manufacturer's protocol.

    Techniques: Expressing, Mouse Assay

    Effect of TCTP on the expression and secretion of HMGB1 in LoVo cells. (A) Immunofluorescence analysis of the expression of HMGB1 following modulation of the expression of TCTP in LoVo cells (magnification, ×400). Green, HMGB1; blue, nuclei; red, F-actin. (B) Immunoblotting analysis of the expression of HMGB1 in the cytoplasm and the culture medium of LoVo cells following modulation of the expression of TCTP. Values were normalized to the internal control GAPDH. ** P

    Journal: International Journal of Oncology

    Article Title: Translationally controlled tumor protein affects colorectal cancer metastasis through the high mobility group box 1-dependent pathway

    doi: 10.3892/ijo.2018.4502

    Figure Lengend Snippet: Effect of TCTP on the expression and secretion of HMGB1 in LoVo cells. (A) Immunofluorescence analysis of the expression of HMGB1 following modulation of the expression of TCTP in LoVo cells (magnification, ×400). Green, HMGB1; blue, nuclei; red, F-actin. (B) Immunoblotting analysis of the expression of HMGB1 in the cytoplasm and the culture medium of LoVo cells following modulation of the expression of TCTP. Values were normalized to the internal control GAPDH. ** P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The HMGB1 concentrations in serum samples were quantified by means of an HMGB1 ELISA Kit II (Shino-Test, Tokyo, Japan) according to the manufacturer's protocol.

    Techniques: Expressing, Immunofluorescence

    High-mobility group box 1 (HMGB1) mediates enhanced lung inflammatory responses following red blood cell (RBC) transfusion. ( A ) Bronchoalveolar lavage (BAL) KC and MIP-2 following transfusion alone, KC P = 0.71, MIP-2 P = 0.90. ( B ) BAL KC and MIP-2 following LPS injection, both * P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Red Blood Cells Induce Necroptosis of Lung Endothelial Cells and Increase Susceptibility to Lung Inflammation

    doi: 10.1164/rccm.201406-1095OC

    Figure Lengend Snippet: High-mobility group box 1 (HMGB1) mediates enhanced lung inflammatory responses following red blood cell (RBC) transfusion. ( A ) Bronchoalveolar lavage (BAL) KC and MIP-2 following transfusion alone, KC P = 0.71, MIP-2 P = 0.90. ( B ) BAL KC and MIP-2 following LPS injection, both * P

    Article Snippet: Supernatants and RBC lysates were collected and HMGB1 concentrations were assayed by ELISA (Chondrex, Redmond, WA) and reported as total HMGB1 per 106 EC.

    Techniques: Injection

    Receptor interacting serine/threonine-protein kinase 3 (RIP3) and high-mobility group box 1 (HMGB1) are increased following red blood cell (RBC) transfusion in mice. ( A ) Proximity ligation assay for RIP1 and RIP3 in the lungs of phosphate-buffered saline (PBS) or RBC transfused mice (n = 6). ( B ) Quantification of PLA puncta, * P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Red Blood Cells Induce Necroptosis of Lung Endothelial Cells and Increase Susceptibility to Lung Inflammation

    doi: 10.1164/rccm.201406-1095OC

    Figure Lengend Snippet: Receptor interacting serine/threonine-protein kinase 3 (RIP3) and high-mobility group box 1 (HMGB1) are increased following red blood cell (RBC) transfusion in mice. ( A ) Proximity ligation assay for RIP1 and RIP3 in the lungs of phosphate-buffered saline (PBS) or RBC transfused mice (n = 6). ( B ) Quantification of PLA puncta, * P

    Article Snippet: Supernatants and RBC lysates were collected and HMGB1 concentrations were assayed by ELISA (Chondrex, Redmond, WA) and reported as total HMGB1 per 106 EC.

    Techniques: Mouse Assay, Proximity Ligation Assay

    Red blood cells (RBCs) induce necroptosis of lung endothelial cells (ECs) and inhibition of necroptosis attenuates lung EC high-mobility group box 1 (HMGB1) release. ( A ) Viability of human lung microvascular ECs following RBC treatment, * P = 0.054. ( B ) HMGB1 release following RBC treatment, P = 0.03. necrostatin (Nec)-1–ZVAD significantly attenuates HMGB1 release with three of the six units tested, P ≤ 0.01. Data representative of three independent studies. ( C ) HMGB1 staining in lung EC 4 hours after RBC treatment or RBC treatment in the presence of Nec-1 (50 μM). red = HMGB1; blue = DAPI; green = F-actin. DAPI = 4′,6-diamidino-2-phenylindole.

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Red Blood Cells Induce Necroptosis of Lung Endothelial Cells and Increase Susceptibility to Lung Inflammation

    doi: 10.1164/rccm.201406-1095OC

    Figure Lengend Snippet: Red blood cells (RBCs) induce necroptosis of lung endothelial cells (ECs) and inhibition of necroptosis attenuates lung EC high-mobility group box 1 (HMGB1) release. ( A ) Viability of human lung microvascular ECs following RBC treatment, * P = 0.054. ( B ) HMGB1 release following RBC treatment, P = 0.03. necrostatin (Nec)-1–ZVAD significantly attenuates HMGB1 release with three of the six units tested, P ≤ 0.01. Data representative of three independent studies. ( C ) HMGB1 staining in lung EC 4 hours after RBC treatment or RBC treatment in the presence of Nec-1 (50 μM). red = HMGB1; blue = DAPI; green = F-actin. DAPI = 4′,6-diamidino-2-phenylindole.

    Article Snippet: Supernatants and RBC lysates were collected and HMGB1 concentrations were assayed by ELISA (Chondrex, Redmond, WA) and reported as total HMGB1 per 106 EC.

    Techniques: Inhibition, Staining

    Red blood cells (RBCs) induce high-mobility group box 1 (HMGB1) release from lung endothelial cells (ECs). ( A ) HMGB1 release following stimulation of human lung microvascular ECs with RBCs for 4 hours, * P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Red Blood Cells Induce Necroptosis of Lung Endothelial Cells and Increase Susceptibility to Lung Inflammation

    doi: 10.1164/rccm.201406-1095OC

    Figure Lengend Snippet: Red blood cells (RBCs) induce high-mobility group box 1 (HMGB1) release from lung endothelial cells (ECs). ( A ) HMGB1 release following stimulation of human lung microvascular ECs with RBCs for 4 hours, * P

    Article Snippet: Supernatants and RBC lysates were collected and HMGB1 concentrations were assayed by ELISA (Chondrex, Redmond, WA) and reported as total HMGB1 per 106 EC.

    Techniques: