Journal: Scientific Reports
Article Title: Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment
Figure Lengend Snippet: Curcumin, but not carnosol, significantly inhibits T cell immune responses in ex-vivo stimulated PBMC from psoriasis patients. PBMC isolated from psoriasis patients (n = 9) were treated with carnosol (5 µM), curcumin (5 µM) or a vehicle control for 6 hours prior to stimulation with anti-CD3. After 4 days, supernatants were removed for analysis of IFNγ and IL-17 concentration by ELISA, and proliferation and cytokine production by T cells was analysed by flow cytometry. CD3 + CD8 - . ( A ) Pooled data depicting mean (±SEM) percentage expression of the proliferation marker Ki67 in CD3 + CD8 - T cells from control, carnosol or curcumin treated PBMC. ( B ) Pooled data depicting mean (±SEM) CD3 + TCRγδ + cells as a percentage of total live cells from control, carnosol or curcumin treated PBMC. ( C ) Pooled data depicting mean (±SEM) percentages of TNFα, IL-2, IFNγ, IL-17A, GM-CSF and IL-22 positive CD3 + CD8 − T cells from control, carnosol or curcumin treated PBMC. ( D ) Pooled data depicting mean (±SEM) concentrations of IFNγ and IL-17 in cell culture supernatants of control, carnosol and curcumin treated PBMC (means of three technical replicates per patient). ( E . ( F ) The cytokine expression profiles of T cells from control, carnosol and curcumin treated psoriasis PBMC were analysed using SPICE software. The pie charts represent the average frequencies of CD3 + CD8 - T cells producing every combination of cytokines for multiple patients (n = 9). The segments within the pie charts denote proportions of the CD3 + CD8 − T cell population producing different combinations of cytokines, and are heat-map coded to indicate increasing cytokine production. The arcs surrounding the pie charts indicate the cytokine(s) produced by that proportion of cells. Statistical significance was determined by one-way ANOVA with Dunnett’s post hoc test to compare carnosol and curcumin treatment to the control (**p
Article Snippet: After 4 days supernatants were removed for analysis of IFNγ and IL-17A concentrations by ELISA (Ready-Set-Go kit; eBioscience), and PBMC were restimulated for flow cytometry.
Techniques: Ex Vivo, Isolation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry, Expressing, Marker, Cell Culture, Software, Produced