immunoglobulin m igm capture antibody Search Results


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  • 99
    Thermo Fisher igm capturing matrix
    Igm Capturing Matrix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore igm antibody
    Vaccination with the Lam-CRM conjugate induces antibody-mediated anti- Candida protection in a murine experimental model of disseminated infection. (a) Anti-β-glucan <t>IgG</t> and <t>IgM</t> titers in Lam-CRM–vaccinated mice. The graph shows the ranges of ELISA titers against the indicated antigens measured in five groups of 6–12 mice (for a total of 46 animals) independently immunized with Lam-CRM. MP, mannoproteins; Pust, pustulan. (b) Survival rates of mice immunized with Lam or CRM or with the Lam-CRM conjugate, as compared with nonimmunized mice (Adj), after a lethal systemic challenge with C. albicans (cumulative data from three independent experiments and 28 mice per group). (c) Fungal burden in the kidneys from four Lam-CRM–vaccinated or four control CRM-vaccinated mice on day 2 after i.v. infection with C. albicans . (d) Number of fungal CFU in kidneys from naive mice given a single administration of anti-Lam-CRM, anti-CRM, or nonimmune (Adj) serum 2 h before an i.v. challenge with C. albicans . Data are from three independent experiments with a total of nine mice per group. (e) Reversal of the passive protection after serum adsorption with Candida cells. The experiment was performed with three mice per group. (f) Effect of the passive vaccination with Protein A affinity–separated fractions of the Lam-CRM serum on Candida kidney load. Data are from three mice per group. Some details on isotype and subclass of anti-β-glucan immunoglobulin in pool A, B and C are given in Fig. S2.
    Igm Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Immunology Consultant Laboratory immunoglobulin m igm capture antibody
    Vaccination with the Lam-CRM conjugate induces antibody-mediated anti- Candida protection in a murine experimental model of disseminated infection. (a) Anti-β-glucan <t>IgG</t> and <t>IgM</t> titers in Lam-CRM–vaccinated mice. The graph shows the ranges of ELISA titers against the indicated antigens measured in five groups of 6–12 mice (for a total of 46 animals) independently immunized with Lam-CRM. MP, mannoproteins; Pust, pustulan. (b) Survival rates of mice immunized with Lam or CRM or with the Lam-CRM conjugate, as compared with nonimmunized mice (Adj), after a lethal systemic challenge with C. albicans (cumulative data from three independent experiments and 28 mice per group). (c) Fungal burden in the kidneys from four Lam-CRM–vaccinated or four control CRM-vaccinated mice on day 2 after i.v. infection with C. albicans . (d) Number of fungal CFU in kidneys from naive mice given a single administration of anti-Lam-CRM, anti-CRM, or nonimmune (Adj) serum 2 h before an i.v. challenge with C. albicans . Data are from three independent experiments with a total of nine mice per group. (e) Reversal of the passive protection after serum adsorption with Candida cells. The experiment was performed with three mice per group. (f) Effect of the passive vaccination with Protein A affinity–separated fractions of the Lam-CRM serum on Candida kidney load. Data are from three mice per group. Some details on isotype and subclass of anti-β-glucan immunoglobulin in pool A, B and C are given in Fig. S2.
    Immunoglobulin M Igm Capture Antibody, supplied by Immunology Consultant Laboratory, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher igm
    Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified <t>IgG</t> at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of <t>IgM,</t> IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.
    Igm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Millipore anti mouse igm capture antibody
    Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified <t>IgG</t> at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of <t>IgM,</t> IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.
    Anti Mouse Igm Capture Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Interchim rabbit antihuman immunoglobulin m antibodies
    Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified <t>IgG</t> at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of <t>IgM,</t> IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.
    Rabbit Antihuman Immunoglobulin M Antibodies, supplied by Interchim, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    SouthernBiotech polyclonal igm capture ab
    Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified <t>IgG</t> at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of <t>IgM,</t> IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.
    Polyclonal Igm Capture Ab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 86/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Boehringer Mannheim anti mouse igm capture antibody
    Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified <t>IgG</t> at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of <t>IgM,</t> IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.
    Anti Mouse Igm Capture Antibody, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SouthernBiotech anti igm
    Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified <t>IgG</t> at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of <t>IgM,</t> IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.
    Anti Igm, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 478 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies igm
    ELISAs were performed for the quantification of Ab to LAM, Ag 85B, and the void fraction of gel-filtered BCG Tokyo culture fluid in healthy Norwegian (NN) ( n = 20), healthy Indian (IN) ( n = 11), and tuberculosis patient (IP) ( n = 17) sera. The plates were coated with 1.0 or 0.5 μg of LAM or 85B, respectively, or a 1/10 dilution of BCG Tokyo void fraction; they were then incubated with a 1/50 dilution of the sera in PBS and developed with <t>HRP-labeled</t> rabbit anti-human IgG plus <t>IgM</t> and H 2 O 2 in ABTS. The negative-control mean values for LAM, Ag 85B and BCG Tokyo void fraction ELISAs run without sera were 13, 10, and 48 optical density (OD) units, respectively.
    Igm, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Interchim rabbit anti human igm antibodies
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    Image Search Results


    Vaccination with the Lam-CRM conjugate induces antibody-mediated anti- Candida protection in a murine experimental model of disseminated infection. (a) Anti-β-glucan IgG and IgM titers in Lam-CRM–vaccinated mice. The graph shows the ranges of ELISA titers against the indicated antigens measured in five groups of 6–12 mice (for a total of 46 animals) independently immunized with Lam-CRM. MP, mannoproteins; Pust, pustulan. (b) Survival rates of mice immunized with Lam or CRM or with the Lam-CRM conjugate, as compared with nonimmunized mice (Adj), after a lethal systemic challenge with C. albicans (cumulative data from three independent experiments and 28 mice per group). (c) Fungal burden in the kidneys from four Lam-CRM–vaccinated or four control CRM-vaccinated mice on day 2 after i.v. infection with C. albicans . (d) Number of fungal CFU in kidneys from naive mice given a single administration of anti-Lam-CRM, anti-CRM, or nonimmune (Adj) serum 2 h before an i.v. challenge with C. albicans . Data are from three independent experiments with a total of nine mice per group. (e) Reversal of the passive protection after serum adsorption with Candida cells. The experiment was performed with three mice per group. (f) Effect of the passive vaccination with Protein A affinity–separated fractions of the Lam-CRM serum on Candida kidney load. Data are from three mice per group. Some details on isotype and subclass of anti-β-glucan immunoglobulin in pool A, B and C are given in Fig. S2.

    Journal: The Journal of Experimental Medicine

    Article Title: A novel glyco-conjugate vaccine against fungal pathogens

    doi: 10.1084/jem.20050749

    Figure Lengend Snippet: Vaccination with the Lam-CRM conjugate induces antibody-mediated anti- Candida protection in a murine experimental model of disseminated infection. (a) Anti-β-glucan IgG and IgM titers in Lam-CRM–vaccinated mice. The graph shows the ranges of ELISA titers against the indicated antigens measured in five groups of 6–12 mice (for a total of 46 animals) independently immunized with Lam-CRM. MP, mannoproteins; Pust, pustulan. (b) Survival rates of mice immunized with Lam or CRM or with the Lam-CRM conjugate, as compared with nonimmunized mice (Adj), after a lethal systemic challenge with C. albicans (cumulative data from three independent experiments and 28 mice per group). (c) Fungal burden in the kidneys from four Lam-CRM–vaccinated or four control CRM-vaccinated mice on day 2 after i.v. infection with C. albicans . (d) Number of fungal CFU in kidneys from naive mice given a single administration of anti-Lam-CRM, anti-CRM, or nonimmune (Adj) serum 2 h before an i.v. challenge with C. albicans . Data are from three independent experiments with a total of nine mice per group. (e) Reversal of the passive protection after serum adsorption with Candida cells. The experiment was performed with three mice per group. (f) Effect of the passive vaccination with Protein A affinity–separated fractions of the Lam-CRM serum on Candida kidney load. Data are from three mice per group. Some details on isotype and subclass of anti-β-glucan immunoglobulin in pool A, B and C are given in Fig. S2.

    Article Snippet: After extensive washings with PBS, slides were treated with fluorescein isothiocyanate–conjugated anti–mouse IgG or IgM antibody (Sigma-Aldrich), washed again, and observed with a Leitz Diaplan fluorescence microscope.

    Techniques: Laser Capture Microdissection, Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay, Adsorption

    Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified IgG at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of IgM, IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.

    Journal: Nature Medicine

    Article Title: IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure

    doi: 10.1038/s41591-019-0441-3

    Figure Lengend Snippet: Qualitatively distinct PPD-specific antibody responses in ‘resisters’ compared with LTBI individuals. a , Graphs depict the AUCs calculated from the ratio of live to total intracellular bacterial burden in primary human monocyte-derived macrophages after treatment with purified IgG at 0.1 mg ml –1 , 0.01 mg ml –1 and 0.001 mg ml –1 (left) from ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39). Extension of analysis to additional donors was performed at a single concentration of purified IgG of 0.1 mg ml –1 due to sample availability (middle). Levels of secreted IL-1β from supernatants were measured by ELISA and are shown relative to no antibody treatment. Purified IgG from individuals in this study, with culture-confirmed pulmonary TB (ATB), is shown as a benchmark. Each line represents one healthy macrophage donor individual. For dot plots, lines are medians. The statistical significance was calculated using Wilcoxon’s matched-pairs signed rank, and two-tailed P values are shown. b , The calculated avidity against PPD from pooled plasma from ‘resisters’ ( n = 40), TST/IGRA-positive LTBI controls ( n = 39) and healthy, HIV-uninfected North Americans ( n = 10) are shown, with lines representing the fitted curves and dotted lines the 95% confidence intervals. Each plasma group was tested in triplicate, with associated calculated avidity represented in the dot plot. The statistical significance was calculated using the Student’s t -test, and a two-tailed P value is indicated. OD, optical density or absorbance. c – e , Plasma from ‘resisters’ ( n = 40) and LTBI controls ( n = 39) was assayed for the ability to mediate: PPD and ESAT6/CFP10-specific, antibody-dependent, monocyte-mediated cellular phagocytosis ( c ); PPD-specific, antibody-dependent neutrophil phagocytosis ( d ); and PPD-specific, NK cell activation by CD107a expression, macrophage inflammatory protein-1β and IFN-γ production ( e ). Data are representative of experiments performed in duplicate over three dilutions. Assays utilizing primary human neutrophils ( d ) and NK cells ( e ) were additionally performed utilizing three independent, healthy, HIV-negative donors. f , Affinity for FcγR2A(R), FcγR2A(H), FcγR3A(V) and FcγR3A(F) were determined using customized Luminex to PPD in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), using plasma diluted at 1:100. MFI is shown on the graph. The statistical significance was calculated using the Mann–Whitney U test, and P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. g , Ratios of plasma levels of IgM, IgG and IgA1 reactive to PPD and LAM in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI controls ( n = 39) are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. h , Ratios of plasma levels of IgG1, IgG2, IgG3 and IgG4 reactive to PPD in ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) were measured by customized multiplex Luminex in serial dilutions. AUCs are depicted with medians and interquartile ranges. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. i , The relative distribution of glycoform substructures isolated from non-antigen-specific and PPD-specific IgG are depicted, with each column representing each individual. j , Principal component analysis demonstrates the overlapping profiles of ‘resisters’ ( n = 40) and TST/IGRA-positive LTBI individuals ( n = 39) in the dominant total glycans isolated from non-antigen-specific IgG compared with partially separating profiles from PPD-specific IgG. ADCP, antibody-dependent cellular phagocytosis; ADNP, antibody-dependent neutrophil phagocytosis; MIP, macrophage inflammatory protein.

    Article Snippet: Briefly, plasma samples were diluted 1:50 in TSM buffer containing 0.05% Tween-20 and 1% BSA and incubated on the slides for 1 h. Slides were washed in TSM wash buffer (TSM-0.05%, Tween-20), and incubated simultaneously with secondary antibodies to IgG and IgM (Invitrogen, 5 μg ml–1 each) for 1 h to detect the presence of anti-glycan antibodies in the plasma.

    Techniques: Derivative Assay, Purification, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Activation Assay, Expressing, Luminex, MANN-WHITNEY, Laser Capture Microdissection, Multiplex Assay, Isolation

    Comparable antibody reactivity to natural, glycan and common pathogens in ‘resisters’ and LTBI controls. a , Plasma levels of IgG and IgM reactive to the common natural antigens cardiolipin, phosphatidylserine (PS) and β 2 -glycoprotein were profiled across ‘resisters’ (RSTR) ( n = 40) and LTBI individuals ( n = 39), represented by MFI using a customized Luminex with medians and interquartile ranges depicted for each group. b , c , Plasma IgG and IgM reactivity to 700 glycans in age- and sex-matched ‘resisters’ ( n = 5) and LTBI individuals ( n = 5) were determined on the NCFGv1 glycan microarray ( b ) and the CFG mammalian-type glycan microarray CFGv5 ( c ). Total fluorescence intensities depicted in heatmaps were determined per individual (rows in b and columns in c ) and plotted in dot plots as relative fluorescence units (RFU), with medians and interquartile ranges depicted for each group. d , Plasma levels of IgG and IgM reactive to Streptococcus pneumoniae capsular polysaccharides (S. pneu.), a mixture of influenza HA, rubella virus, tetanus toxoid, VZV and CMV pp65 in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), were determined using customized multiplex Luminex. AUCs were determined from MFIs generated by three dilutions and plotted for each individual with medians and interquartile ranges depicted for each group. For a – d , statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. e , Principal component analysis using the IgG and IgM data generated in d demonstrates overlapping dot plots of microbial-reactive antibody profiles in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39). The loadings plot and PLSDA plot are mirror images. Thus, the geographic location of the 12 antibody features on the loadings plot reflects the subject’s group in which a particular feature is enriched.

    Journal: Nature Medicine

    Article Title: IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure

    doi: 10.1038/s41591-019-0441-3

    Figure Lengend Snippet: Comparable antibody reactivity to natural, glycan and common pathogens in ‘resisters’ and LTBI controls. a , Plasma levels of IgG and IgM reactive to the common natural antigens cardiolipin, phosphatidylserine (PS) and β 2 -glycoprotein were profiled across ‘resisters’ (RSTR) ( n = 40) and LTBI individuals ( n = 39), represented by MFI using a customized Luminex with medians and interquartile ranges depicted for each group. b , c , Plasma IgG and IgM reactivity to 700 glycans in age- and sex-matched ‘resisters’ ( n = 5) and LTBI individuals ( n = 5) were determined on the NCFGv1 glycan microarray ( b ) and the CFG mammalian-type glycan microarray CFGv5 ( c ). Total fluorescence intensities depicted in heatmaps were determined per individual (rows in b and columns in c ) and plotted in dot plots as relative fluorescence units (RFU), with medians and interquartile ranges depicted for each group. d , Plasma levels of IgG and IgM reactive to Streptococcus pneumoniae capsular polysaccharides (S. pneu.), a mixture of influenza HA, rubella virus, tetanus toxoid, VZV and CMV pp65 in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39), were determined using customized multiplex Luminex. AUCs were determined from MFIs generated by three dilutions and plotted for each individual with medians and interquartile ranges depicted for each group. For a – d , statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. e , Principal component analysis using the IgG and IgM data generated in d demonstrates overlapping dot plots of microbial-reactive antibody profiles in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39). The loadings plot and PLSDA plot are mirror images. Thus, the geographic location of the 12 antibody features on the loadings plot reflects the subject’s group in which a particular feature is enriched.

    Article Snippet: Briefly, plasma samples were diluted 1:50 in TSM buffer containing 0.05% Tween-20 and 1% BSA and incubated on the slides for 1 h. Slides were washed in TSM wash buffer (TSM-0.05%, Tween-20), and incubated simultaneously with secondary antibodies to IgG and IgM (Invitrogen, 5 μg ml–1 each) for 1 h to detect the presence of anti-glycan antibodies in the plasma.

    Techniques: Luminex, Microarray, Fluorescence, Multiplex Assay, Generated, MANN-WHITNEY, Two Tailed Test

    Detectable Mtb -specific humoral immunity in ‘resisters’. a – c , Plasma levels of IgM ( a ), IgG ( b ) and IgA1 ( c ) reactive to PPD, Ag85A, ESAT6 and CFP10, α-crystalline (HspX), GroES and LAM were quantified in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39) with AUCs determined from MFIs generated using a customized Luminex assay, generated with three dilutions and plotted for each individual with medians and interquartile ranges depicted for each group. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers.

    Journal: Nature Medicine

    Article Title: IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure

    doi: 10.1038/s41591-019-0441-3

    Figure Lengend Snippet: Detectable Mtb -specific humoral immunity in ‘resisters’. a – c , Plasma levels of IgM ( a ), IgG ( b ) and IgA1 ( c ) reactive to PPD, Ag85A, ESAT6 and CFP10, α-crystalline (HspX), GroES and LAM were quantified in ‘resisters’ ( n = 40) and LTBI individuals ( n = 39) with AUCs determined from MFIs generated using a customized Luminex assay, generated with three dilutions and plotted for each individual with medians and interquartile ranges depicted for each group. The statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers.

    Article Snippet: Briefly, plasma samples were diluted 1:50 in TSM buffer containing 0.05% Tween-20 and 1% BSA and incubated on the slides for 1 h. Slides were washed in TSM wash buffer (TSM-0.05%, Tween-20), and incubated simultaneously with secondary antibodies to IgG and IgM (Invitrogen, 5 μg ml–1 each) for 1 h to detect the presence of anti-glycan antibodies in the plasma.

    Techniques: Laser Capture Microdissection, Generated, Luminex, MANN-WHITNEY, Two Tailed Test

    ELISAs were performed for the quantification of Ab to LAM, Ag 85B, and the void fraction of gel-filtered BCG Tokyo culture fluid in healthy Norwegian (NN) ( n = 20), healthy Indian (IN) ( n = 11), and tuberculosis patient (IP) ( n = 17) sera. The plates were coated with 1.0 or 0.5 μg of LAM or 85B, respectively, or a 1/10 dilution of BCG Tokyo void fraction; they were then incubated with a 1/50 dilution of the sera in PBS and developed with HRP-labeled rabbit anti-human IgG plus IgM and H 2 O 2 in ABTS. The negative-control mean values for LAM, Ag 85B and BCG Tokyo void fraction ELISAs run without sera were 13, 10, and 48 optical density (OD) units, respectively.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Involvement of Antilipoarabinomannan Antibodies in Classical Complement Activation in Tuberculosis

    doi:

    Figure Lengend Snippet: ELISAs were performed for the quantification of Ab to LAM, Ag 85B, and the void fraction of gel-filtered BCG Tokyo culture fluid in healthy Norwegian (NN) ( n = 20), healthy Indian (IN) ( n = 11), and tuberculosis patient (IP) ( n = 17) sera. The plates were coated with 1.0 or 0.5 μg of LAM or 85B, respectively, or a 1/10 dilution of BCG Tokyo void fraction; they were then incubated with a 1/50 dilution of the sera in PBS and developed with HRP-labeled rabbit anti-human IgG plus IgM and H 2 O 2 in ABTS. The negative-control mean values for LAM, Ag 85B and BCG Tokyo void fraction ELISAs run without sera were 13, 10, and 48 optical density (OD) units, respectively.

    Article Snippet: The labeled Abs were horseradish peroxidase (HRP)–sheep anti-human IgG and IgM (Dako), HRP–mouse anti-human IgG1 to -4 (Zymed, San Francisco, Calif.), HRP–donkey anti-rabbit Ig (Amersham International, Amersham, United Kingdom), HRP–sheep anti-human Ig (Amersham), mouse MAb to human C6 biotinylated in our laboratory, fluorescein isothiocyanate (FITC)–rabbit anti-human C3c (Dako), FITC–goat anti-rabbit IgG, and phycoerythrin (PE)–goat anti-mouse Ig (Southern Biotechnology Associates, Inc., Birmingham, Ala.).

    Techniques: Laser Capture Microdissection, Incubation, Labeling, Negative Control

    Scatter blot comparing the results of the InBios and the Panbio IgM capture ELISA with the results of the EUROIMMUN IgM IIFT. To facilitate the readability one IIFT outlier (1∶320) was taken out of the diagram. Test values: Panbio IgM capture ELISA results (▪), InBios IgM capture ELISA results (⧫). Trend line: Panbio IgM capture ELISA results (thin), InBios IgM capture ELISA results (thick).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Evaluation of Serological Diagnostic Test Systems Assessing the Immune Response to Japanese Encephalitis Vaccination

    doi: 10.1371/journal.pntd.0000883

    Figure Lengend Snippet: Scatter blot comparing the results of the InBios and the Panbio IgM capture ELISA with the results of the EUROIMMUN IgM IIFT. To facilitate the readability one IIFT outlier (1∶320) was taken out of the diagram. Test values: Panbio IgM capture ELISA results (▪), InBios IgM capture ELISA results (⧫). Trend line: Panbio IgM capture ELISA results (thin), InBios IgM capture ELISA results (thick).

    Article Snippet: In the IgM-capture ELISA 50 µL of the 1∶100 diluted sera were used in each well.

    Techniques: Enzyme-linked Immunosorbent Assay

    Scatter blot comparing the results of the PRNT50 with the results of both IgM ELISAs. Test values: Panbio IgM ELISA (⧫), InBios IgM ELISA (▪). Trend line: Panbio IgM ELISA (thin), InBios IgM ELISA (thick).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Evaluation of Serological Diagnostic Test Systems Assessing the Immune Response to Japanese Encephalitis Vaccination

    doi: 10.1371/journal.pntd.0000883

    Figure Lengend Snippet: Scatter blot comparing the results of the PRNT50 with the results of both IgM ELISAs. Test values: Panbio IgM ELISA (⧫), InBios IgM ELISA (▪). Trend line: Panbio IgM ELISA (thin), InBios IgM ELISA (thick).

    Article Snippet: In the IgM-capture ELISA 50 µL of the 1∶100 diluted sera were used in each well.

    Techniques: Enzyme-linked Immunosorbent Assay

    Induction of WNV-specific IgM and IgG responses by one dose of RepliVAX WN.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of RepliVAX WN, a Single-Cycle Flavivirus Vaccine, in a Non-Human Primate Model of West Nile Virus Infection

    doi: 10.4269/ajtmh.2010.09-0310

    Figure Lengend Snippet: Induction of WNV-specific IgM and IgG responses by one dose of RepliVAX WN.

    Article Snippet: West Nile virus–specific IgM and IgG levels in serum samples were analyzed by using the WNV IgM Capture DxSelect and IgG DxSelect ELISA kits (Focus Diagnostics, Cypress, CA) according to manufacturer's protocols.

    Techniques:

    Distribution of WNV cases among horses, livestock animals, and wildlife species during 2008–2015 and of WNV neutralizing antibody‒positive veterinarians involved in equine, wildlife, and livestock disease management during 2011‒2012, South Africa. A) Samples were collected from horses during 2008–2015 and from livestock and wildlife 2010–2015. Samples were considered positive if they tested positive for WNV genome by PCR or for WNV IgM by WNV IgM Capture ELISA (IDEXX Laboratories, Montpellier, France) and WNV neutralizing antibody by neutralization assay. B) Distribution of veterinarians described in previous report ( 11 ). Human serum was considered positive if virus neutralization was observed at a titer of 1:10 and higher. WNV, West Nile virus.

    Journal: Emerging Infectious Diseases

    Article Title: West Nile Virus Lineage 2 in Horses and Other Animals with Neurologic Disease, South Africa, 2008–2015

    doi: 10.3201/eid2312.162078

    Figure Lengend Snippet: Distribution of WNV cases among horses, livestock animals, and wildlife species during 2008–2015 and of WNV neutralizing antibody‒positive veterinarians involved in equine, wildlife, and livestock disease management during 2011‒2012, South Africa. A) Samples were collected from horses during 2008–2015 and from livestock and wildlife 2010–2015. Samples were considered positive if they tested positive for WNV genome by PCR or for WNV IgM by WNV IgM Capture ELISA (IDEXX Laboratories, Montpellier, France) and WNV neutralizing antibody by neutralization assay. B) Distribution of veterinarians described in previous report ( 11 ). Human serum was considered positive if virus neutralization was observed at a titer of 1:10 and higher. WNV, West Nile virus.

    Article Snippet: We screened all equine serum and plasma specimens for WNV IgM (WNV IgM Capture ELISA Test; IDEXX Laboratories, Montpellier, France) and confirmed by neutralization assay ( ).

    Techniques: Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Neutralization

    Antigen uptake and reception of T cell help ex vivois not impaired in Itsn2-/- B cells. ( A–B ) Purified naïve WT or Itsn2 -/- B cells were labelled on ice with soluble biotinylated anti-IgM ( A ) or anti-IgM coated particles ( B ), and incubated at 37°C for the indicated times, prior to fixation. Extracellular antigen was then detected by flow cytometry. Data are quantified on the right panel as percentage of initial MFI for each sample ( A ), or the percentage of the cells with external beads ( B ). Data are representative of more than 3 independent experiments with 2 mice in each group. ( C ) Purified naïve WT or Itsn2 -/- B cells were stimulated with beads coated with anti-IgM and Eα peptide, and expression of MHCII-Eα conjugates measured by flow cytometry 5 hr after stimulation. Data are quantified on the right panel and show the Eα presentation for increasing amounts of Eα on the beads. ( D–E ) CTV-labelled WT or Itsn2 -/- B cells were stimulated with beads coated with IgM and OVA, and co-cultured with CFSE-labelled OTII T cells. Expression of CD40, CD86 and CD69 was measured by flow cytometry at d1 and d2 ( D ). B and T cell proliferation rates according were characterised to CFSE and CTV dilution at d3 of the culture ( E ). ( F ) CFSE-labelled WT and CTV-labelled Itsn2 -/- MD4 B cells were transferred adoptively into WT recipients, and after 24 hr, animals were injected i.v. with fluorescently-labelled HEL-coated particles. Beads internalisation was measured by flow cytometry 24 hr post-injection.

    Journal: eLife

    Article Title: Tuning of in vivo cognate B-T cell interactions by Intersectin 2 is required for effective anti-viral B cell immunity

    doi: 10.7554/eLife.26556

    Figure Lengend Snippet: Antigen uptake and reception of T cell help ex vivois not impaired in Itsn2-/- B cells. ( A–B ) Purified naïve WT or Itsn2 -/- B cells were labelled on ice with soluble biotinylated anti-IgM ( A ) or anti-IgM coated particles ( B ), and incubated at 37°C for the indicated times, prior to fixation. Extracellular antigen was then detected by flow cytometry. Data are quantified on the right panel as percentage of initial MFI for each sample ( A ), or the percentage of the cells with external beads ( B ). Data are representative of more than 3 independent experiments with 2 mice in each group. ( C ) Purified naïve WT or Itsn2 -/- B cells were stimulated with beads coated with anti-IgM and Eα peptide, and expression of MHCII-Eα conjugates measured by flow cytometry 5 hr after stimulation. Data are quantified on the right panel and show the Eα presentation for increasing amounts of Eα on the beads. ( D–E ) CTV-labelled WT or Itsn2 -/- B cells were stimulated with beads coated with IgM and OVA, and co-cultured with CFSE-labelled OTII T cells. Expression of CD40, CD86 and CD69 was measured by flow cytometry at d1 and d2 ( D ). B and T cell proliferation rates according were characterised to CFSE and CTV dilution at d3 of the culture ( E ). ( F ) CFSE-labelled WT and CTV-labelled Itsn2 -/- MD4 B cells were transferred adoptively into WT recipients, and after 24 hr, animals were injected i.v. with fluorescently-labelled HEL-coated particles. Beads internalisation was measured by flow cytometry 24 hr post-injection.

    Article Snippet: NP-specific antibody titres were detected by ELISA, using NP20-BSA or NP4-BSA for capture, and biotinylated anti–mouse IgM (Southern Biotech, Birmingham, Alabama), IgG (Southern Biotech), IgG2b (Southern Biotech), IgG2c (Southern Biotech), and IgG3 (BD, Franklin Lakes, New Jersey) for detection.

    Techniques: Purification, Incubation, Flow Cytometry, Cytometry, Mouse Assay, Expressing, Cell Culture, Injection