immunofluorescence microscopy Search Results


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    • dapi  (Thermo Fisher)
    99
    Thermo Fisher dapi
    Y-27632 and paclitaxel impact osteogenic/adipogenic gene expression. a Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−Y) or treated with 10 μM Y-27632 (+Y, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). b Representative micrograph of hMSC (5 × 10 6 cells mL −1 ) encapsulated in a 1:0.75 hydrogel after 28 days showing fibronectin, α 5 integrin, and <t>DAPI.</t> Fibronectin co-localized with punctate α 5 integrin by Manders’ coefficients: Manders’ tM1 = 0.83 ± 0.18 and Manders’ tM2 = 0.84 ± 0.10 ( n = 4, 25 cells). c Representative micrographs of hMSC within hydrogels after 72 h and stained with <t>Phalloidin-TRITC</t> (actin) and DAPI. d Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−PTX) or treated with 50 nM paclitaxel (+PTX, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). e Representative micrographs of hMSC (5 × 10 6 cells mL −1 ) within a 1:0.75 hydrogel for 72 h and stained for tubulin and DAPI. Scale bars in b , c , and e are 100 µm, and in insets = 10 µm. In a and d a Mann–Whitney test (two-tailed) was used to detect statistical significance, * p
    Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dapi - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier
    dapi  (Millipore)
    99
    Millipore dapi
    Y-27632 and paclitaxel impact osteogenic/adipogenic gene expression. a Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−Y) or treated with 10 μM Y-27632 (+Y, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). b Representative micrograph of hMSC (5 × 10 6 cells mL −1 ) encapsulated in a 1:0.75 hydrogel after 28 days showing fibronectin, α 5 integrin, and <t>DAPI.</t> Fibronectin co-localized with punctate α 5 integrin by Manders’ coefficients: Manders’ tM1 = 0.83 ± 0.18 and Manders’ tM2 = 0.84 ± 0.10 ( n = 4, 25 cells). c Representative micrographs of hMSC within hydrogels after 72 h and stained with <t>Phalloidin-TRITC</t> (actin) and DAPI. d Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−PTX) or treated with 50 nM paclitaxel (+PTX, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). e Representative micrographs of hMSC (5 × 10 6 cells mL −1 ) within a 1:0.75 hydrogel for 72 h and stained for tubulin and DAPI. Scale bars in b , c , and e are 100 µm, and in insets = 10 µm. In a and d a Mann–Whitney test (two-tailed) was used to detect statistical significance, * p
    Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dapi - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier
    monoclonal anti flag m2 antibody  (Millipore)
    99
    Millipore monoclonal anti flag m2 antibody
    Y-27632 and paclitaxel impact osteogenic/adipogenic gene expression. a Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−Y) or treated with 10 μM Y-27632 (+Y, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). b Representative micrograph of hMSC (5 × 10 6 cells mL −1 ) encapsulated in a 1:0.75 hydrogel after 28 days showing fibronectin, α 5 integrin, and <t>DAPI.</t> Fibronectin co-localized with punctate α 5 integrin by Manders’ coefficients: Manders’ tM1 = 0.83 ± 0.18 and Manders’ tM2 = 0.84 ± 0.10 ( n = 4, 25 cells). c Representative micrographs of hMSC within hydrogels after 72 h and stained with <t>Phalloidin-TRITC</t> (actin) and DAPI. d Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−PTX) or treated with 50 nM paclitaxel (+PTX, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). e Representative micrographs of hMSC (5 × 10 6 cells mL −1 ) within a 1:0.75 hydrogel for 72 h and stained for tubulin and DAPI. Scale bars in b , c , and e are 100 µm, and in insets = 10 µm. In a and d a Mann–Whitney test (two-tailed) was used to detect statistical significance, * p
    Monoclonal Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti flag m2 antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti flag m2 antibody - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier
    monoclonal anti alpha tubulin antibody  (Millipore)
    99
    Millipore monoclonal anti alpha tubulin antibody
    Y-27632 and paclitaxel impact osteogenic/adipogenic gene expression. a Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−Y) or treated with 10 μM Y-27632 (+Y, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). b Representative micrograph of hMSC (5 × 10 6 cells mL −1 ) encapsulated in a 1:0.75 hydrogel after 28 days showing fibronectin, α 5 integrin, and <t>DAPI.</t> Fibronectin co-localized with punctate α 5 integrin by Manders’ coefficients: Manders’ tM1 = 0.83 ± 0.18 and Manders’ tM2 = 0.84 ± 0.10 ( n = 4, 25 cells). c Representative micrographs of hMSC within hydrogels after 72 h and stained with <t>Phalloidin-TRITC</t> (actin) and DAPI. d Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−PTX) or treated with 50 nM paclitaxel (+PTX, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). e Representative micrographs of hMSC (5 × 10 6 cells mL −1 ) within a 1:0.75 hydrogel for 72 h and stained for tubulin and DAPI. Scale bars in b , c , and e are 100 µm, and in insets = 10 µm. In a and d a Mann–Whitney test (two-tailed) was used to detect statistical significance, * p
    Monoclonal Anti Alpha Tubulin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti alpha tubulin antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti alpha tubulin antibody - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Y-27632 and paclitaxel impact osteogenic/adipogenic gene expression. a Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−Y) or treated with 10 μM Y-27632 (+Y, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). b Representative micrograph of hMSC (5 × 10 6 cells mL −1 ) encapsulated in a 1:0.75 hydrogel after 28 days showing fibronectin, α 5 integrin, and DAPI. Fibronectin co-localized with punctate α 5 integrin by Manders’ coefficients: Manders’ tM1 = 0.83 ± 0.18 and Manders’ tM2 = 0.84 ± 0.10 ( n = 4, 25 cells). c Representative micrographs of hMSC within hydrogels after 72 h and stained with Phalloidin-TRITC (actin) and DAPI. d Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−PTX) or treated with 50 nM paclitaxel (+PTX, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). e Representative micrographs of hMSC (5 × 10 6 cells mL −1 ) within a 1:0.75 hydrogel for 72 h and stained for tubulin and DAPI. Scale bars in b , c , and e are 100 µm, and in insets = 10 µm. In a and d a Mann–Whitney test (two-tailed) was used to detect statistical significance, * p

    Journal: Nature Communications

    Article Title: Bi-directional cell-pericellular matrix interactions direct stem cell fate

    doi: 10.1038/s41467-018-06183-4

    Figure Lengend Snippet: Y-27632 and paclitaxel impact osteogenic/adipogenic gene expression. a Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−Y) or treated with 10 μM Y-27632 (+Y, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). b Representative micrograph of hMSC (5 × 10 6 cells mL −1 ) encapsulated in a 1:0.75 hydrogel after 28 days showing fibronectin, α 5 integrin, and DAPI. Fibronectin co-localized with punctate α 5 integrin by Manders’ coefficients: Manders’ tM1 = 0.83 ± 0.18 and Manders’ tM2 = 0.84 ± 0.10 ( n = 4, 25 cells). c Representative micrographs of hMSC within hydrogels after 72 h and stained with Phalloidin-TRITC (actin) and DAPI. d Gene expression analyses for markers of adipogenesis ( PPARγ and C/EPBα ) and osteogenesis ( RUNX2 and BGLAP ) in hMSC cultured for 72 h with basal culture medium (−PTX) or treated with 50 nM paclitaxel (+PTX, n ≥ 8). Expression levels (mean + s.d.) are shown as fold change normalized to expression in undifferentiated hMSC (set to 1). e Representative micrographs of hMSC (5 × 10 6 cells mL −1 ) within a 1:0.75 hydrogel for 72 h and stained for tubulin and DAPI. Scale bars in b , c , and e are 100 µm, and in insets = 10 µm. In a and d a Mann–Whitney test (two-tailed) was used to detect statistical significance, * p

    Article Snippet: The efficiency of hydrogel decellularization was confirmed by fluorescence microscopy by staining with 1 μg mL−1 4′,6-diamidino-2-phenylindole (DAPI, #D3571, Molecular Probes), and 1:100 Phalloidin-Tetramethylrhodamine B isothiocyanate (TRITC) from Amanita phalloides (#P1951, Sigma).

    Techniques: Expressing, Cell Culture, Staining, MANN-WHITNEY, Two Tailed Test