immunofluorescence assays Search Results


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  • 92
    GE Healthcare hrp labeled iggs
    Hrp Labeled Iggs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunofluorescence
    Immunofluorescence, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore immunofluorescence analysis
    Immunofluorescence Analysis, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunofluorescence applications
    Immunofluorescence Applications, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam immunofluorescence assays
    Immunofluorescence Assays, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GraphPad Prism Inc immunofluorescence images
    Immunofluorescence Images, supplied by GraphPad Prism Inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    3-D Matrix immunofluorescence imaging
    Immunofluorescence Imaging, supplied by 3-D Matrix, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio immunofluorescence kit
    Immunofluorescence Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss immunofluorescence microscope
    Immunofluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    KEYENCE immunofluorescence microscope
    Immunofluorescence Microscope, supplied by KEYENCE, used in various techniques. Bioz Stars score: 92/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nikon immunofluorescence microscope
    Immunofluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Olympus immunofluorescence microscope
    Immunofluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 95/100, based on 628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher immunofluorescence mitotracker
    Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with <t>Mitotracker</t> Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.
    Immunofluorescence Mitotracker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Polysciences inc immunofluorescence slides
    Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with <t>Mitotracker</t> Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.
    Immunofluorescence Slides, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 89/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    EUROIMMUN hep2 immunofluorescence
    Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with <t>Mitotracker</t> Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.
    Hep2 Immunofluorescence, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher lamp1 immunofluorescence
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Lamp1 Immunofluorescence, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avantor immunofluorescence analysis
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Immunofluorescence Analysis, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Jackson Immuno immunofluorescence detection
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Immunofluorescence Detection, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies immunofluorescence panel
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Immunofluorescence Panel, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    PerkinElmer multiplex immunofluorescence
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Multiplex Immunofluorescence, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Abcam immunofluorescence analysis
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Immunofluorescence Analysis, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno immunofluorescence analysis
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Immunofluorescence Analysis, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Zeiss immunofluorescence apparatus
    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) <t>LAMP1</t> immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).
    Immunofluorescence Apparatus, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 85/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with Mitotracker Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.

    Journal: PLoS ONE

    Article Title: Abolition of Peroxiredoxin-5 Mitochondrial Targeting during Canid Evolution

    doi: 10.1371/journal.pone.0072844

    Figure Lengend Snippet: Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with Mitotracker Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.

    Article Snippet: Immunofluorescence Mitotracker (Molecular Probes) staining and immunofluorescence were performed as described previously , using 1∶200 rabbit anti-human PRDX5 and 1∶50 FITC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc.).

    Techniques: Northern Blot, Construct, Clone Assay, Expressing, Plasmid Preparation, Transfection, Staining, Sequencing

    Overexpression of human PRDX5 in MDCK cells. (A) Organization of the construct cloned into mammalian expression vector pEF-BOS. EF-1α prom, promoter region of human EF-1α chromosomal gene; G-CSF poly(A), polyadenylation signal from human granulocyte colony-stimulating factor. (B) Representation of the proteins encoded by the different constructs used for transfection. Mito-hum and Mito-hum-C47A correspond to the enzymatically active and inactive mitochondrial human PRDX5 with the cleavable (scissors) presequence (MTS), respectively. Cyto-hum and Cyto-hum-C47S correspond to enzymatically active and inactive cytosolic human PRDX5, respectively. PRDX5 content of each MDCK clone was verified by Western blotting (C) and total expression levels were quantified (D). PRDX5 protein levels were normalized with β-actin and were expressed in relative units of PRDX5 content in MDCK control cells. Values are means ± SEM of triplicates. The subcellular localization of PRDX5 in the MDCK clones was verified by immunofluorescence (E). Mitochondria were stained with MitoTracker Red prior to cell fixation. Cell nuclei were counterstained with DAPI. Hs PRDX5: human PRDX5.

    Journal: PLoS ONE

    Article Title: Abolition of Peroxiredoxin-5 Mitochondrial Targeting during Canid Evolution

    doi: 10.1371/journal.pone.0072844

    Figure Lengend Snippet: Overexpression of human PRDX5 in MDCK cells. (A) Organization of the construct cloned into mammalian expression vector pEF-BOS. EF-1α prom, promoter region of human EF-1α chromosomal gene; G-CSF poly(A), polyadenylation signal from human granulocyte colony-stimulating factor. (B) Representation of the proteins encoded by the different constructs used for transfection. Mito-hum and Mito-hum-C47A correspond to the enzymatically active and inactive mitochondrial human PRDX5 with the cleavable (scissors) presequence (MTS), respectively. Cyto-hum and Cyto-hum-C47S correspond to enzymatically active and inactive cytosolic human PRDX5, respectively. PRDX5 content of each MDCK clone was verified by Western blotting (C) and total expression levels were quantified (D). PRDX5 protein levels were normalized with β-actin and were expressed in relative units of PRDX5 content in MDCK control cells. Values are means ± SEM of triplicates. The subcellular localization of PRDX5 in the MDCK clones was verified by immunofluorescence (E). Mitochondria were stained with MitoTracker Red prior to cell fixation. Cell nuclei were counterstained with DAPI. Hs PRDX5: human PRDX5.

    Article Snippet: Immunofluorescence Mitotracker (Molecular Probes) staining and immunofluorescence were performed as described previously , using 1∶200 rabbit anti-human PRDX5 and 1∶50 FITC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc.).

    Techniques: Over Expression, Construct, Clone Assay, Expressing, Plasmid Preparation, Transfection, Western Blot, Immunofluorescence, Staining

    Dog PRDX5 subcellular distribution. (A) Intracellular localization of dog PRDX5 was assessed by liver subcellular fractionation. Post-nuclear (E), cytosolic (S), mitochondrial (Mito) and peroxisomal (Perox) fractions were analyzed for marker enzyme and PRDX5 content by Western blotting, using anti-Hsp90 (cytosolic), anti-COX4 (mitochondrial), anti-catalase (peroxisomal) and anti-PRDX5 antibodies. Fifteen µg of proteins from each fraction were loaded. PRDX5 subcellular localization was also assessed by immunofluorescence detection of endogenous PRDX5 in MDCK cells (B, E) with co-localization with MitoTracker Red (C) or with peroxisomal catalase (F). Nuclei were counterstained with DAPI. Hsp90 , heat shock protein 90; COX4 , cytochrome c oxidase subunit 4.

    Journal: PLoS ONE

    Article Title: Abolition of Peroxiredoxin-5 Mitochondrial Targeting during Canid Evolution

    doi: 10.1371/journal.pone.0072844

    Figure Lengend Snippet: Dog PRDX5 subcellular distribution. (A) Intracellular localization of dog PRDX5 was assessed by liver subcellular fractionation. Post-nuclear (E), cytosolic (S), mitochondrial (Mito) and peroxisomal (Perox) fractions were analyzed for marker enzyme and PRDX5 content by Western blotting, using anti-Hsp90 (cytosolic), anti-COX4 (mitochondrial), anti-catalase (peroxisomal) and anti-PRDX5 antibodies. Fifteen µg of proteins from each fraction were loaded. PRDX5 subcellular localization was also assessed by immunofluorescence detection of endogenous PRDX5 in MDCK cells (B, E) with co-localization with MitoTracker Red (C) or with peroxisomal catalase (F). Nuclei were counterstained with DAPI. Hsp90 , heat shock protein 90; COX4 , cytochrome c oxidase subunit 4.

    Article Snippet: Immunofluorescence Mitotracker (Molecular Probes) staining and immunofluorescence were performed as described previously , using 1∶200 rabbit anti-human PRDX5 and 1∶50 FITC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc.).

    Techniques: Fractionation, Marker, Western Blot, Immunofluorescence

    M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) LAMP1 immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).

    Journal: PLoS ONE

    Article Title: Interactions between Na?ve and Infected Macrophages Reduce Mycobacterium tuberculosis Viability

    doi: 10.1371/journal.pone.0027972

    Figure Lengend Snippet: M.tb localizes to LC3-positive compartments. ( A ) GFP-LC3 expressing macrophages were infected with mCherry- M.tb (MOI 50 or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding ( arrowhead ) around mCherry- M.tb and co-localization indicated by yellow pixels ( arrow ) that are not seen in macrophages challenged at low MOI. ( B ) LAMP1 immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers ( arrowheads ).

    Article Snippet: Secondary antibody Alexa Fluor 647 goat-anti-rabbit IgG detection for LAMP1 immunofluorescence was purchased from Molecular Probes.

    Techniques: Expressing, Infection, Confocal Microscopy, Immunostaining