immunofluorescence assay Search Results


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  • 92
    GE Healthcare hrp labeled iggs
    Hrp Labeled Iggs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore immunofluorescence analysis
    Immunofluorescence Analysis, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1566 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Zeiss immunofluorescence apparatus
    Immunofluorescence Apparatus, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 85/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunofluorescence assays
    Immunofluorescence Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Human Protein Atlas immunofluorescence images
    Immunofluorescence Images, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nikon immunofluorescence images
    Immunofluorescence Images, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 2058 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc immunofluorescence lc3
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Lc3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Zeiss immunofluorescence microscope
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    KEYENCE immunofluorescence microscope
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Microscope, supplied by KEYENCE, used in various techniques. Bioz Stars score: 92/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Leica Microsystems immunofluorescence microscope
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 90/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nikon immunofluorescence microscope
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Olympus immunofluorescence microscope
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 858 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunofluorescence microscope
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Microscope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TechLab Inc immunofluorescence test
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Test, supplied by TechLab Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunofluorescence visualization
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Visualization, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc immunofluorescence
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunofluorescence applications
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Applications, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam immunofluorescence assays
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Assays, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GraphPad Prism Inc immunofluorescence images
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Images, supplied by GraphPad Prism Inc, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    3-D Matrix immunofluorescence imaging
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Imaging, supplied by 3-D Matrix, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio immunofluorescence kit
    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of <t>LC3.</t> The arrow indicates the autophagosomes (* P
    Immunofluorescence Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunofluorescence mitotracker
    Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with <t>Mitotracker</t> Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.
    Immunofluorescence Mitotracker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Polysciences inc immunofluorescence slides
    Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with <t>Mitotracker</t> Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.
    Immunofluorescence Slides, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of LC3. The arrow indicates the autophagosomes (* P

    Journal: Cell Death & Disease

    Article Title: The responsively decreased PKM2 facilitates the survival of pancreatic cancer cells in hypoglucose

    doi: 10.1038/s41419-017-0158-5

    Figure Lengend Snippet: Knockdown of AMPKα1 expression reversed the effects of reduced PKM2 on BXPC-3-LG cells a The survival of BXPC-3-LG was tested after transfected with siPKM2, siAMPKα1, and co-transfected with both of them for 5 days. b The expression of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR. c PKM2, AMPKα1, and autophagy-related proteins were represented by western blot (upper); the histogram showed the intensity ratio of LC3II/LC3I (lower). d Immunofluorescence was performed to test the autophagosomes in cells with the antibody of LC3. The arrow indicates the autophagosomes (* P

    Article Snippet: Immunofluorescence LC3 (Cell Signaling Technology), CD31 (Goodbio Technology Co., Ltd, China), and PKM2 (Cell Signaling Technology) for immunofluorescence were diluted into 1:200, 1:50, and 1:200 respectively.

    Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Immunofluorescence

    Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with Mitotracker Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.

    Journal: PLoS ONE

    Article Title: Abolition of Peroxiredoxin-5 Mitochondrial Targeting during Canid Evolution

    doi: 10.1371/journal.pone.0072844

    Figure Lengend Snippet: Subcellular localization of giant panda (A–D) and northern elephant seal (E–H) PRDX5 MTS expressed in fusion with GFP in MDCK cells. Structure of the constructs cloned into mammalian expression vector pcDNA3.1 and used for MDCK transfection (A and E). Both alternative ATG initiation codons are indicated (arrows). Transfected cells were examined for GFP fusion expression (B and F). Mitochondria were stained with Mitotracker Red (C and G) and nuclei were counterstained with DAPI. BGH polyA , bovine growth hormone polyadenylation signal; GFP , green fluorescent protein; MTS, mitochondrial targeting sequence; pCMV , promoter of cytomegalovirus.

    Article Snippet: Immunofluorescence Mitotracker (Molecular Probes) staining and immunofluorescence were performed as described previously , using 1∶200 rabbit anti-human PRDX5 and 1∶50 FITC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc.).

    Techniques: Northern Blot, Construct, Clone Assay, Expressing, Plasmid Preparation, Transfection, Staining, Sequencing

    Overexpression of human PRDX5 in MDCK cells. (A) Organization of the construct cloned into mammalian expression vector pEF-BOS. EF-1α prom, promoter region of human EF-1α chromosomal gene; G-CSF poly(A), polyadenylation signal from human granulocyte colony-stimulating factor. (B) Representation of the proteins encoded by the different constructs used for transfection. Mito-hum and Mito-hum-C47A correspond to the enzymatically active and inactive mitochondrial human PRDX5 with the cleavable (scissors) presequence (MTS), respectively. Cyto-hum and Cyto-hum-C47S correspond to enzymatically active and inactive cytosolic human PRDX5, respectively. PRDX5 content of each MDCK clone was verified by Western blotting (C) and total expression levels were quantified (D). PRDX5 protein levels were normalized with β-actin and were expressed in relative units of PRDX5 content in MDCK control cells. Values are means ± SEM of triplicates. The subcellular localization of PRDX5 in the MDCK clones was verified by immunofluorescence (E). Mitochondria were stained with MitoTracker Red prior to cell fixation. Cell nuclei were counterstained with DAPI. Hs PRDX5: human PRDX5.

    Journal: PLoS ONE

    Article Title: Abolition of Peroxiredoxin-5 Mitochondrial Targeting during Canid Evolution

    doi: 10.1371/journal.pone.0072844

    Figure Lengend Snippet: Overexpression of human PRDX5 in MDCK cells. (A) Organization of the construct cloned into mammalian expression vector pEF-BOS. EF-1α prom, promoter region of human EF-1α chromosomal gene; G-CSF poly(A), polyadenylation signal from human granulocyte colony-stimulating factor. (B) Representation of the proteins encoded by the different constructs used for transfection. Mito-hum and Mito-hum-C47A correspond to the enzymatically active and inactive mitochondrial human PRDX5 with the cleavable (scissors) presequence (MTS), respectively. Cyto-hum and Cyto-hum-C47S correspond to enzymatically active and inactive cytosolic human PRDX5, respectively. PRDX5 content of each MDCK clone was verified by Western blotting (C) and total expression levels were quantified (D). PRDX5 protein levels were normalized with β-actin and were expressed in relative units of PRDX5 content in MDCK control cells. Values are means ± SEM of triplicates. The subcellular localization of PRDX5 in the MDCK clones was verified by immunofluorescence (E). Mitochondria were stained with MitoTracker Red prior to cell fixation. Cell nuclei were counterstained with DAPI. Hs PRDX5: human PRDX5.

    Article Snippet: Immunofluorescence Mitotracker (Molecular Probes) staining and immunofluorescence were performed as described previously , using 1∶200 rabbit anti-human PRDX5 and 1∶50 FITC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc.).

    Techniques: Over Expression, Construct, Clone Assay, Expressing, Plasmid Preparation, Transfection, Western Blot, Immunofluorescence, Staining

    Dog PRDX5 subcellular distribution. (A) Intracellular localization of dog PRDX5 was assessed by liver subcellular fractionation. Post-nuclear (E), cytosolic (S), mitochondrial (Mito) and peroxisomal (Perox) fractions were analyzed for marker enzyme and PRDX5 content by Western blotting, using anti-Hsp90 (cytosolic), anti-COX4 (mitochondrial), anti-catalase (peroxisomal) and anti-PRDX5 antibodies. Fifteen µg of proteins from each fraction were loaded. PRDX5 subcellular localization was also assessed by immunofluorescence detection of endogenous PRDX5 in MDCK cells (B, E) with co-localization with MitoTracker Red (C) or with peroxisomal catalase (F). Nuclei were counterstained with DAPI. Hsp90 , heat shock protein 90; COX4 , cytochrome c oxidase subunit 4.

    Journal: PLoS ONE

    Article Title: Abolition of Peroxiredoxin-5 Mitochondrial Targeting during Canid Evolution

    doi: 10.1371/journal.pone.0072844

    Figure Lengend Snippet: Dog PRDX5 subcellular distribution. (A) Intracellular localization of dog PRDX5 was assessed by liver subcellular fractionation. Post-nuclear (E), cytosolic (S), mitochondrial (Mito) and peroxisomal (Perox) fractions were analyzed for marker enzyme and PRDX5 content by Western blotting, using anti-Hsp90 (cytosolic), anti-COX4 (mitochondrial), anti-catalase (peroxisomal) and anti-PRDX5 antibodies. Fifteen µg of proteins from each fraction were loaded. PRDX5 subcellular localization was also assessed by immunofluorescence detection of endogenous PRDX5 in MDCK cells (B, E) with co-localization with MitoTracker Red (C) or with peroxisomal catalase (F). Nuclei were counterstained with DAPI. Hsp90 , heat shock protein 90; COX4 , cytochrome c oxidase subunit 4.

    Article Snippet: Immunofluorescence Mitotracker (Molecular Probes) staining and immunofluorescence were performed as described previously , using 1∶200 rabbit anti-human PRDX5 and 1∶50 FITC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc.).

    Techniques: Fractionation, Marker, Western Blot, Immunofluorescence