imac columns Affibody Search Results


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  • 94
    LI-COR irdye 800cw maleimide
    Comparison of plasma excretion for the two proteins. Plasma excretion data with error bars and bi-exponential curve fits to the data are shown for <t>cetuximab-IRDye</t> 680RD (left) and Affibody-IRDye <t>800CW</t> (right). Curve fit equations are also shown where FL is fluorescence intensity. R-squared values of 0.71 and 0.90 for cetuximab and Affibody fits respectively.
    Irdye 800cw Maleimide, supplied by LI-COR, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher alexa fluor 488 c5 maleimide
    Comparison of plasma excretion for the two proteins. Plasma excretion data with error bars and bi-exponential curve fits to the data are shown for <t>cetuximab-IRDye</t> 680RD (left) and Affibody-IRDye <t>800CW</t> (right). Curve fit equations are also shown where FL is fluorescence intensity. R-squared values of 0.71 and 0.90 for cetuximab and Affibody fits respectively.
    Alexa Fluor 488 C5 Maleimide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Affibody affibody conjugate
    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR <t>affibody</t> probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).
    Affibody Conjugate, supplied by Affibody, used in various techniques. Bioz Stars score: 88/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Merck KGaA tlc silica gel 60 f254
    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR <t>affibody</t> probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).
    Tlc Silica Gel 60 F254, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA thin layer chromatography tlc
    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR <t>affibody</t> probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).
    Thin Layer Chromatography Tlc, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 1157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Affibody fluorescence imaging probes
    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR <t>affibody</t> probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).
    Fluorescence Imaging Probes, supplied by Affibody, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Dojindo Labs icg maleimide derivative i
    Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with <t>ICG</t> <t>maleimide</t> or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).
    Icg Maleimide Derivative I, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies 1200 hplc system
    Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with <t>ICG</t> <t>maleimide</t> or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).
    1200 Hplc System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 5707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Affibody 99m tc
    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of <t>99m</t> Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).
    99m Tc, supplied by Affibody, used in various techniques. Bioz Stars score: 91/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Affibody 99m
    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of <t>99m</t> Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).
    99m, supplied by Affibody, used in various techniques. Bioz Stars score: 89/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Affibody affibody binder
    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of <t>99m</t> Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).
    Affibody Binder, supplied by Affibody, used in various techniques. Bioz Stars score: 85/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher alexa fluor 647 dye
    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of <t>99m</t> Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).
    Alexa Fluor 647 Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher alexa fluor 680 c2 maleimide
    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of <t>99m</t> Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).
    Alexa Fluor 680 C2 Maleimide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher alexa fluor 750
    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of <t>99m</t> Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).
    Alexa Fluor 750, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Affibody anti egfr affibody conjugated peg chains
    Representative pictures from H E stained sections of the heart, lung, liver, spleen, kidney and small intestine, harvested from mice at 120 d following intravenous administration of different doses of <t>anti-EGFR-PEG-TiO</t> 2 -UCNs. Black arrows denote clusters of macrophages with ingested nanoparticles. Magnification: 200X, scale bar: 50 µm.
    Anti Egfr Affibody Conjugated Peg Chains, supplied by Affibody, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Affibody anti egfr affibody
    The receptor concentration determined using <t>anti-EGFR</t> <t>affibody</t> RCI is compared to four standard methods used to determine protein concentration: A) pathologist scored ex vivo immunohistochemistry (IHC, r = 0.69, p = 2×10 −5 , m = 0.29 ± 0.06, b = 0.8 ± 0.2), B) ex vivo immunofluorescence ( r = 0.62, p = 5×10 −4 , m = 1.0 ± 0.2, b = 2.1 ± 0.9), C) ex vivo Western blot ( r = 0.35, p = 0.08, m = 0.04 ± 0.03, b = 0.06 ± 0.08), and D) in vitro flow cytometry ( r = 0.43, p = 0.017, m = 9×10 4 ± 3×10 4 , intercept = 4×10 4 ± 1.3×10 5 ). The linear regression (solid line) is displayed as well as the 95% confidence bands (dashed lines) and the 95% prediction bands (dotted lines).
    Anti Egfr Affibody, supplied by Affibody, used in various techniques. Bioz Stars score: 89/100, based on 231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher alexa fluor 555 succinimidyl ester
    Electrophilic affibody improves stability and has high specificity at the mammalian cell surface. COS7 cells expressing ZSPA-TM N6C or N6C F13E were labeled with biotinylated affibody D36C with or without EBA. Affibody binding was detected with <t>streptavidin-Alexa</t> <t>Fluor</t> 555 ( red ), and this staining is overlaid with cyan fluorescent protein co-transfection marker ( blue ) and bright field images. Scale bar , 20 μm.
    Alexa Fluor 555 Succinimidyl Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher dylight 755
    The tumor-targeting ability of Z HPV16 E7 affitoxin384. The xenograft-bearing mice were generated with cell lines of (A) SiHa, (C) CaSki, (E) HeLa, and (G) A375, respectively. NIR imaging was performed at different time points post injection with <t>DyLight-755-labeled</t> Z HPV16 E7 affitoxin384. DyLight-755-labeled Z HPV16 E7 384 and Z wt affitoxin were used as positive and negative controls, respectively. The tumor/muscle ratios were calculated at 8 h post-injection of indicated agents in xenograft-bearing mice generated with cell lines of (B) SiHa, (D) CaSki, (F) HeLa, and (H) A375, respectively. Data are given as mean ± SD (n=5). ** P
    Dylight 755, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Affibody egfr targeting affibody
    The tumor-targeting ability of Z HPV16 E7 affitoxin384. The xenograft-bearing mice were generated with cell lines of (A) SiHa, (C) CaSki, (E) HeLa, and (G) A375, respectively. NIR imaging was performed at different time points post injection with <t>DyLight-755-labeled</t> Z HPV16 E7 affitoxin384. DyLight-755-labeled Z HPV16 E7 384 and Z wt affitoxin were used as positive and negative controls, respectively. The tumor/muscle ratios were calculated at 8 h post-injection of indicated agents in xenograft-bearing mice generated with cell lines of (B) SiHa, (D) CaSki, (F) HeLa, and (H) A375, respectively. Data are given as mean ± SD (n=5). ** P
    Egfr Targeting Affibody, supplied by Affibody, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Labconco freeze dry system
    The tumor-targeting ability of Z HPV16 E7 affitoxin384. The xenograft-bearing mice were generated with cell lines of (A) SiHa, (C) CaSki, (E) HeLa, and (G) A375, respectively. NIR imaging was performed at different time points post injection with <t>DyLight-755-labeled</t> Z HPV16 E7 affitoxin384. DyLight-755-labeled Z HPV16 E7 384 and Z wt affitoxin were used as positive and negative controls, respectively. The tumor/muscle ratios were calculated at 8 h post-injection of indicated agents in xenograft-bearing mice generated with cell lines of (B) SiHa, (D) CaSki, (F) HeLa, and (H) A375, respectively. Data are given as mean ± SD (n=5). ** P
    Freeze Dry System, supplied by Labconco, used in various techniques. Bioz Stars score: 89/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Labconco freezone plus
    The tumor-targeting ability of Z HPV16 E7 affitoxin384. The xenograft-bearing mice were generated with cell lines of (A) SiHa, (C) CaSki, (E) HeLa, and (G) A375, respectively. NIR imaging was performed at different time points post injection with <t>DyLight-755-labeled</t> Z HPV16 E7 affitoxin384. DyLight-755-labeled Z HPV16 E7 384 and Z wt affitoxin were used as positive and negative controls, respectively. The tumor/muscle ratios were calculated at 8 h post-injection of indicated agents in xenograft-bearing mice generated with cell lines of (B) SiHa, (D) CaSki, (F) HeLa, and (H) A375, respectively. Data are given as mean ± SD (n=5). ** P
    Freezone Plus, supplied by Labconco, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Affibody her2 neu
    Functional evaluation of the <t>Her2</t> affibody-SPIO conjugates. <t>Her2/neu-positive</t> and Her2/neu-negative cells were incubated with Her2-SPIO conjugates in the presence and absence of excess free affibody. Free affibody served as a competitive inhibitor to confirm specific binding of the Her2/neu receptor. Relaxivity measurements and T2*-weighted MR images of each cell suspension were acquired.
    Her2 Neu, supplied by Affibody, used in various techniques. Bioz Stars score: 92/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher hispur cobalt resin
    Functional evaluation of the <t>Her2</t> affibody-SPIO conjugates. <t>Her2/neu-positive</t> and Her2/neu-negative cells were incubated with Her2-SPIO conjugates in the presence and absence of excess free affibody. Free affibody served as a competitive inhibitor to confirm specific binding of the Her2/neu receptor. Relaxivity measurements and T2*-weighted MR images of each cell suspension were acquired.
    Hispur Cobalt Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hispur ni nta resin
    Functional evaluation of the <t>Her2</t> affibody-SPIO conjugates. <t>Her2/neu-positive</t> and Her2/neu-negative cells were incubated with Her2-SPIO conjugates in the presence and absence of excess free affibody. Free affibody served as a competitive inhibitor to confirm specific binding of the Her2/neu receptor. Relaxivity measurements and T2*-weighted MR images of each cell suspension were acquired.
    Hispur Ni Nta Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare mabselect sure
    Functional evaluation of the <t>Her2</t> affibody-SPIO conjugates. <t>Her2/neu-positive</t> and Her2/neu-negative cells were incubated with Her2-SPIO conjugates in the presence and absence of excess free affibody. Free affibody served as a competitive inhibitor to confirm specific binding of the Her2/neu receptor. Relaxivity measurements and T2*-weighted MR images of each cell suspension were acquired.
    Mabselect Sure, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Comparison of plasma excretion for the two proteins. Plasma excretion data with error bars and bi-exponential curve fits to the data are shown for cetuximab-IRDye 680RD (left) and Affibody-IRDye 800CW (right). Curve fit equations are also shown where FL is fluorescence intensity. R-squared values of 0.71 and 0.90 for cetuximab and Affibody fits respectively.

    Journal: PLoS ONE

    Article Title: Fluorescent Affibody Peptide Penetration in Glioma Margin Is Superior to Full Antibody

    doi: 10.1371/journal.pone.0060390

    Figure Lengend Snippet: Comparison of plasma excretion for the two proteins. Plasma excretion data with error bars and bi-exponential curve fits to the data are shown for cetuximab-IRDye 680RD (left) and Affibody-IRDye 800CW (right). Curve fit equations are also shown where FL is fluorescence intensity. R-squared values of 0.71 and 0.90 for cetuximab and Affibody fits respectively.

    Article Snippet: The fluorophore, IRDye 800CW maleimide (LI-COR Biosciences, Lincoln, Nebraska), was suspended in pure water at approximately 2 mg/ml, and was added to the protein solution to achieve a 2.5 molar excess of dye to protein as recommended by LI-COR.

    Techniques: Fluorescence

    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR affibody probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR affibody probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Dissection, Staining, Imaging, Mouse Assay, Injection, Autoradiography, Radioactivity, Fluorescence

    Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with ICG maleimide or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with ICG maleimide or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Binding Assay, Labeling, Thin Layer Chromatography, Fluorescence

    Near-infrared (NIR) imaging of cell lines by the addition of affibody probes to conditioned medium ( a , b ). SAS cells showed strong fluorescence signals of anti-EGFR affibody imaging probes ( a , left). SAS cells ( a , middle) expressed higher anti-epidermal growth factor receptor (EGFR) levels than MCF-7 cells ( a , right). SK-BR3 cells showed stronger fluorescence signals in anti-HER2 affibody imaging probes than MDA-MB231 cells ( b ). Histological section study ( c ). Anti-HER2 affibody probe was administered to histological sections of lymph nodes from breast cancer patients. Metastatic cancer cells are shown after hematoxylin and eosin (H E) staining ( c , upper row, left). Human epidermal growth factor receptor 2 (HER2) expression was positive in metastatic cancer cells by immunohistochemical staining ( c , upper row, middle). High-intensity NIR signals from the probe identically corresponded with the area of increased HER2 expression ( c , upper row, right). In the HER2-negative metastatic lymph node section ( c , lower row), immunohistochemical staining for HER2 and NIR signals were not visible in metastatic cancer cells ( c , lower row, middle, right). Scale bar in ( a ): 20 μm. Scale bar in ( c ): 200 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Near-infrared (NIR) imaging of cell lines by the addition of affibody probes to conditioned medium ( a , b ). SAS cells showed strong fluorescence signals of anti-EGFR affibody imaging probes ( a , left). SAS cells ( a , middle) expressed higher anti-epidermal growth factor receptor (EGFR) levels than MCF-7 cells ( a , right). SK-BR3 cells showed stronger fluorescence signals in anti-HER2 affibody imaging probes than MDA-MB231 cells ( b ). Histological section study ( c ). Anti-HER2 affibody probe was administered to histological sections of lymph nodes from breast cancer patients. Metastatic cancer cells are shown after hematoxylin and eosin (H E) staining ( c , upper row, left). Human epidermal growth factor receptor 2 (HER2) expression was positive in metastatic cancer cells by immunohistochemical staining ( c , upper row, middle). High-intensity NIR signals from the probe identically corresponded with the area of increased HER2 expression ( c , upper row, right). In the HER2-negative metastatic lymph node section ( c , lower row), immunohistochemical staining for HER2 and NIR signals were not visible in metastatic cancer cells ( c , lower row, middle, right). Scale bar in ( a ): 20 μm. Scale bar in ( c ): 200 μm.

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Fluorescence, Multiple Displacement Amplification, Staining, Expressing, Immunohistochemistry

    Imaging of metastatic cancer cells in lymph nodes. ( a ) In a mouse lymph node metastasis model, an anti-EGFR affibody probe was injected into the mouse tongue 24 h prior to sacrifice and lymph node dissection. Six lymph nodes were excised; three lymph nodes were highly fluorescent, and the remaining three lymph nodes were not fluorescent. Immunohistochemical staining for EGFR was found in fluorescence-positive lymph nodes (lymph node 2 (R), lymph node 3 (R)). EGFR expression was not visible in the nonfluorescent lymph node (lymph node 2 (L)). ( b ) Two lymph nodes, one from each side of the mouse, were dissected 24 h after anti-EGFR affibody probe injection into the tongue. The indocyanine green (ICG) fluorescence signal was obvious and corresponded to the immunohistochemically stained EGFR expression in the lymph nodes (red circles and arrows). The panel on the right is a magnified view of the two lymph nodes in the left panel of the NIR images.

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Imaging of metastatic cancer cells in lymph nodes. ( a ) In a mouse lymph node metastasis model, an anti-EGFR affibody probe was injected into the mouse tongue 24 h prior to sacrifice and lymph node dissection. Six lymph nodes were excised; three lymph nodes were highly fluorescent, and the remaining three lymph nodes were not fluorescent. Immunohistochemical staining for EGFR was found in fluorescence-positive lymph nodes (lymph node 2 (R), lymph node 3 (R)). EGFR expression was not visible in the nonfluorescent lymph node (lymph node 2 (L)). ( b ) Two lymph nodes, one from each side of the mouse, were dissected 24 h after anti-EGFR affibody probe injection into the tongue. The indocyanine green (ICG) fluorescence signal was obvious and corresponded to the immunohistochemically stained EGFR expression in the lymph nodes (red circles and arrows). The panel on the right is a magnified view of the two lymph nodes in the left panel of the NIR images.

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Injection, Dissection, Immunohistochemistry, Staining, Fluorescence, Expressing

    Dynamic imaging study. ( a ) NIR images showed changes in the signal from the anti-EGFR affibody probe in the lymph nodes of a normal control mouse (left). The fluorescence signal intensity was examined at 0.5, 1, 2, 3, 4, 6, and 24 h after the tongue injection in two mice (total four lymph nodes). The peak intensity was observed at one and two hours after the injection (right). The error bar shows the standard deviation. ( b ) The left panels are images of metastatic and nonmetastatic lymph nodes. Weak, almost equal NIR signal intensity was found in two nonmetastatic lymph nodes at 0.5 and 1 h post-injection. The signal intensity almost disappeared at 24 h after the injection (top, left). A high signal intensity remained in the metastatic lymph node (arrow) at 24 h after injection (bottom, left). The time-dependent NIR signal intensity of the anti-EGFR affibody probe is represented as a percentage of the initial signal intensity (30 min). The signal intensity ratio was greater for metastatic lymph nodes than nonmetastatic lymph nodes at 1, 2, 3, and 6 h after the injection (right panel). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Dynamic imaging study. ( a ) NIR images showed changes in the signal from the anti-EGFR affibody probe in the lymph nodes of a normal control mouse (left). The fluorescence signal intensity was examined at 0.5, 1, 2, 3, 4, 6, and 24 h after the tongue injection in two mice (total four lymph nodes). The peak intensity was observed at one and two hours after the injection (right). The error bar shows the standard deviation. ( b ) The left panels are images of metastatic and nonmetastatic lymph nodes. Weak, almost equal NIR signal intensity was found in two nonmetastatic lymph nodes at 0.5 and 1 h post-injection. The signal intensity almost disappeared at 24 h after the injection (top, left). A high signal intensity remained in the metastatic lymph node (arrow) at 24 h after injection (bottom, left). The time-dependent NIR signal intensity of the anti-EGFR affibody probe is represented as a percentage of the initial signal intensity (30 min). The signal intensity ratio was greater for metastatic lymph nodes than nonmetastatic lymph nodes at 1, 2, 3, and 6 h after the injection (right panel). * p

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Fluorescence, Injection, Mouse Assay, Standard Deviation

    ( a ) Image of a custom-built illuminator (light emitting diode (LED) emission: peak wavelength, 690 nm). Image of SAS xenograft tumor in the back of a mouse after anti-EGFR affibody photoimmunotherapy (PIT). The image was captured one hour after the probe was injected into the right tumor. The contralateral xenograft tumor served as a control (right). ( b ) The CCK-8 assay showed that the combination of the EGFR affibody IR700 probe and NIR irradiation decreased the survival rate of SAS cells rather than exposure to NIR or the EGFR affibody IR700 probe alone ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: ( a ) Image of a custom-built illuminator (light emitting diode (LED) emission: peak wavelength, 690 nm). Image of SAS xenograft tumor in the back of a mouse after anti-EGFR affibody photoimmunotherapy (PIT). The image was captured one hour after the probe was injected into the right tumor. The contralateral xenograft tumor served as a control (right). ( b ) The CCK-8 assay showed that the combination of the EGFR affibody IR700 probe and NIR irradiation decreased the survival rate of SAS cells rather than exposure to NIR or the EGFR affibody IR700 probe alone ( p

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Injection, CCK-8 Assay, Irradiation

    Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with ICG maleimide or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with ICG maleimide or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Article Snippet: ICG-maleimide derivative I (1 mg, 50 nmol/tube, Dojindo, Kumamoto, Japan) was dissolved in 1 mL of dimethyl sulfoxide (DMSO).

    Techniques: Binding Assay, Labeling, Thin Layer Chromatography, Fluorescence

    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of 99m Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).

    Journal: International Journal of Oncology

    Article Title: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule

    doi: 10.3892/ijo.2014.2782

    Figure Lengend Snippet: Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of 99m Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).

    Article Snippet: When the ITLC strips were eluted with PBS, pertechnetate, as well as carbonyl and histidine complexes of 99m Tc, migrated with the eluent front (Rf =1.0), while Affibody molecules did not move under these conditions (Rf =0.0).

    Techniques: Imaging, Expressing, Mouse Assay, Injection, Derivative Assay

    Cellular processing of 99m Tc-(HE) 3 -ZCAIX:1 by CAIX-expressing SK-RC-52 cells in vitro . Cell bound activity is normalized to the maximum uptake. Data are presented as mean values for six cell dishes and standard deviations. Error bars might be smaller than the symbols.

    Journal: International Journal of Oncology

    Article Title: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule

    doi: 10.3892/ijo.2014.2782

    Figure Lengend Snippet: Cellular processing of 99m Tc-(HE) 3 -ZCAIX:1 by CAIX-expressing SK-RC-52 cells in vitro . Cell bound activity is normalized to the maximum uptake. Data are presented as mean values for six cell dishes and standard deviations. Error bars might be smaller than the symbols.

    Article Snippet: When the ITLC strips were eluted with PBS, pertechnetate, as well as carbonyl and histidine complexes of 99m Tc, migrated with the eluent front (Rf =1.0), while Affibody molecules did not move under these conditions (Rf =0.0).

    Techniques: Expressing, In Vitro, Activity Assay

    In vivo binding specificity of 99m Tc-(HE) 3 -ZCAIX:1 in NMRI nu/nu mice bearing SK-RC-52 xenografts at 4 h after injection. Blocked group was subcutaneously preinjected with a large excess amount of unlabeled Affibody. Results are presented as the mean ± standard deviation for 4 animals.

    Journal: International Journal of Oncology

    Article Title: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule

    doi: 10.3892/ijo.2014.2782

    Figure Lengend Snippet: In vivo binding specificity of 99m Tc-(HE) 3 -ZCAIX:1 in NMRI nu/nu mice bearing SK-RC-52 xenografts at 4 h after injection. Blocked group was subcutaneously preinjected with a large excess amount of unlabeled Affibody. Results are presented as the mean ± standard deviation for 4 animals.

    Article Snippet: When the ITLC strips were eluted with PBS, pertechnetate, as well as carbonyl and histidine complexes of 99m Tc, migrated with the eluent front (Rf =1.0), while Affibody molecules did not move under these conditions (Rf =0.0).

    Techniques: In Vivo, Binding Assay, Mouse Assay, Injection, Standard Deviation

    Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of 99m Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).

    Journal: International Journal of Oncology

    Article Title: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule

    doi: 10.3892/ijo.2014.2782

    Figure Lengend Snippet: Imaging of CAIX-expressing SK-RC-52 xenografts in NMRI nu/nu mice using clinical gamma-camera. Image was acquired at 4 h after injection of 99m Tc-(HE) 3 -ZCAIX:1. Contours were derived from a digital photograph and superimposed over image to facilitate interpretation. Arrows point at tumors (T) and kidneys (K).

    Article Snippet: Labeling of Affibody molecules with [99m Tc(CO)3 ]+ and 125 I 99m Tc-(HE)3 -ZCAIX:1 Affibody molecules were efficiently labeled with 99m Tc.

    Techniques: Imaging, Expressing, Mouse Assay, Injection, Derivative Assay

    Cellular processing of 99m Tc-(HE) 3 -ZCAIX:1 by CAIX-expressing SK-RC-52 cells in vitro . Cell bound activity is normalized to the maximum uptake. Data are presented as mean values for six cell dishes and standard deviations. Error bars might be smaller than the symbols.

    Journal: International Journal of Oncology

    Article Title: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule

    doi: 10.3892/ijo.2014.2782

    Figure Lengend Snippet: Cellular processing of 99m Tc-(HE) 3 -ZCAIX:1 by CAIX-expressing SK-RC-52 cells in vitro . Cell bound activity is normalized to the maximum uptake. Data are presented as mean values for six cell dishes and standard deviations. Error bars might be smaller than the symbols.

    Article Snippet: Labeling of Affibody molecules with [99m Tc(CO)3 ]+ and 125 I 99m Tc-(HE)3 -ZCAIX:1 Affibody molecules were efficiently labeled with 99m Tc.

    Techniques: Expressing, In Vitro, Activity Assay

    In vivo binding specificity of 99m Tc-(HE) 3 -ZCAIX:1 in NMRI nu/nu mice bearing SK-RC-52 xenografts at 4 h after injection. Blocked group was subcutaneously preinjected with a large excess amount of unlabeled Affibody. Results are presented as the mean ± standard deviation for 4 animals.

    Journal: International Journal of Oncology

    Article Title: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule

    doi: 10.3892/ijo.2014.2782

    Figure Lengend Snippet: In vivo binding specificity of 99m Tc-(HE) 3 -ZCAIX:1 in NMRI nu/nu mice bearing SK-RC-52 xenografts at 4 h after injection. Blocked group was subcutaneously preinjected with a large excess amount of unlabeled Affibody. Results are presented as the mean ± standard deviation for 4 animals.

    Article Snippet: Labeling of Affibody molecules with [99m Tc(CO)3 ]+ and 125 I 99m Tc-(HE)3 -ZCAIX:1 Affibody molecules were efficiently labeled with 99m Tc.

    Techniques: In Vivo, Binding Assay, Mouse Assay, Injection, Standard Deviation

    Representative pictures from H E stained sections of the heart, lung, liver, spleen, kidney and small intestine, harvested from mice at 120 d following intravenous administration of different doses of anti-EGFR-PEG-TiO 2 -UCNs. Black arrows denote clusters of macrophages with ingested nanoparticles. Magnification: 200X, scale bar: 50 µm.

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: Representative pictures from H E stained sections of the heart, lung, liver, spleen, kidney and small intestine, harvested from mice at 120 d following intravenous administration of different doses of anti-EGFR-PEG-TiO 2 -UCNs. Black arrows denote clusters of macrophages with ingested nanoparticles. Magnification: 200X, scale bar: 50 µm.

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Staining, Mouse Assay

    Differential expression of various cytokines in the plasma of mice at 10, 30 and 60 min post injection of 50mg/kg anti-EGFR-PEG-TiO 2 -UCNs.* P

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: Differential expression of various cytokines in the plasma of mice at 10, 30 and 60 min post injection of 50mg/kg anti-EGFR-PEG-TiO 2 -UCNs.* P

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Expressing, Mouse Assay, Injection

    Representative H E stained images of the major organs (heart, lung, liver, spleen, kidney and small intestine) in mice harvested at different time-points (24 h to 28 d) following intravenous administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCNs. Magnification: 200×, Scale bar: 50 µm.

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: Representative H E stained images of the major organs (heart, lung, liver, spleen, kidney and small intestine) in mice harvested at different time-points (24 h to 28 d) following intravenous administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCNs. Magnification: 200×, Scale bar: 50 µm.

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Staining, Mouse Assay

    Representative images of UCL imaging and the corresponding fluorescence images (Green represents the plasma membrane and red represents nucleus) of cryosections of various organs harvested at (A) 5 min, (B) 4 h and (C) 24 h following systemic administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN. Magnification: 200X, Scale bar: 20 µm.

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: Representative images of UCL imaging and the corresponding fluorescence images (Green represents the plasma membrane and red represents nucleus) of cryosections of various organs harvested at (A) 5 min, (B) 4 h and (C) 24 h following systemic administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN. Magnification: 200X, Scale bar: 20 µm.

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Imaging, Fluorescence

    (A) Comparison of UCN uptake at different time-points in tumor tissues by ICP-AES analysis, following systemic administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN. Data are mean (n=2-3) ± SD. Image of sections of tumor tissue excised from Evan's blue stained mouse treated with (B) NIR light alone and (C) NIR light irradiation 4 h post systemic administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN. (D) Tumor volumes charted against days to assess the tumor response in various treatment groups. Data are mean (n= 4-5 per group) ± SD. (E) Survival rates of mice in different treatment groups within 60 d.

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: (A) Comparison of UCN uptake at different time-points in tumor tissues by ICP-AES analysis, following systemic administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN. Data are mean (n=2-3) ± SD. Image of sections of tumor tissue excised from Evan's blue stained mouse treated with (B) NIR light alone and (C) NIR light irradiation 4 h post systemic administration of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN. (D) Tumor volumes charted against days to assess the tumor response in various treatment groups. Data are mean (n= 4-5 per group) ± SD. (E) Survival rates of mice in different treatment groups within 60 d.

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Staining, Irradiation, Mouse Assay

    (A) Representative images of nanoparticle uptake (blue fluorescence) in OSCC cells post 3 h incubation; green (Alexa Fluor ® 488) and red (Propidium iodide (PI)) fluorescence indicate cell membrane and nucleus respectively (Magnification: 200X, Scale bar: 20 μm). (B) Comparison of fluorescence intensities of 1 mM unmodified and anti-EGFR-affibody conjugated TiO 2 -UCNs internalized by OSCC cells. *P

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: (A) Representative images of nanoparticle uptake (blue fluorescence) in OSCC cells post 3 h incubation; green (Alexa Fluor ® 488) and red (Propidium iodide (PI)) fluorescence indicate cell membrane and nucleus respectively (Magnification: 200X, Scale bar: 20 μm). (B) Comparison of fluorescence intensities of 1 mM unmodified and anti-EGFR-affibody conjugated TiO 2 -UCNs internalized by OSCC cells. *P

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Fluorescence, Incubation

    (A) In vitro dark toxicity of nanoparticles incubated with OSCC cells for 6 h using MTT assay. (B) OSCC cell viability 24 h following in vitro PDT; control cells are untreated cells assumed to have 100% viability, light alone control are cells treated with NIR light alone. (C) In vitro PDT following incubation of anti-EGFR-PEG-TiO 2 -UCNs with various cells for a brief period of 1 h, to demonstrate selective cell killing; * P

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: (A) In vitro dark toxicity of nanoparticles incubated with OSCC cells for 6 h using MTT assay. (B) OSCC cell viability 24 h following in vitro PDT; control cells are untreated cells assumed to have 100% viability, light alone control are cells treated with NIR light alone. (C) In vitro PDT following incubation of anti-EGFR-PEG-TiO 2 -UCNs with various cells for a brief period of 1 h, to demonstrate selective cell killing; * P

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: In Vitro, Incubation, MTT Assay

    (A) Rate of hemolysis in RBCs upon 2 h incubation with nanoparticles at incremental concentrations, data are mean (n=2) ± SD. (B) Platelet activation assay presented in terms of concentration of PF4 in mouse blood plasma following intravenous administration of anti-EGFR-PEG-TiO 2 -UCNs, data are mean (n=3) ± SD. (C) Comparison of level of Prothrombin in blood plasma following intravenous injection of different doses of anti-EGFR-PEG-TiO 2 -UCNs; * P

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: (A) Rate of hemolysis in RBCs upon 2 h incubation with nanoparticles at incremental concentrations, data are mean (n=2) ± SD. (B) Platelet activation assay presented in terms of concentration of PF4 in mouse blood plasma following intravenous administration of anti-EGFR-PEG-TiO 2 -UCNs, data are mean (n=3) ± SD. (C) Comparison of level of Prothrombin in blood plasma following intravenous injection of different doses of anti-EGFR-PEG-TiO 2 -UCNs; * P

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Incubation, Activation Assay, Concentration Assay, Injection

    (A) Schematic of synthesis of anti-EGFR-PEG-TiO 2 -UCN. (B) TEM image of anti-EGFR-PEG-TiO 2 -UCN (scale bar: 50 nm). (C) Average hydrodynamic size of 100 μg/ml anti-EGFR-PEG-TiO 2 -UCN soaked in different media at room temperature (RT) plotted as a function of time the nanoparticles were soaked. (D) Fluorescence emission spectra of 100 μg/ml anti-EGFR-PEG-TiO 2 -UCN in PBS irradiated immediately by NIR light and irradiated 24 h after soaking in PBS. (E) FT-IR absorption spectra of TiO 2 -UCN and anti-EGFR-PEG-TiO 2 -UCN. (F) ROS production from irradiated and non-irradiated anti-EGFR-PEG-TiO 2 -UCNs in PBS and corresponding unmodified TiO 2 -UCNs; * P

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: (A) Schematic of synthesis of anti-EGFR-PEG-TiO 2 -UCN. (B) TEM image of anti-EGFR-PEG-TiO 2 -UCN (scale bar: 50 nm). (C) Average hydrodynamic size of 100 μg/ml anti-EGFR-PEG-TiO 2 -UCN soaked in different media at room temperature (RT) plotted as a function of time the nanoparticles were soaked. (D) Fluorescence emission spectra of 100 μg/ml anti-EGFR-PEG-TiO 2 -UCN in PBS irradiated immediately by NIR light and irradiated 24 h after soaking in PBS. (E) FT-IR absorption spectra of TiO 2 -UCN and anti-EGFR-PEG-TiO 2 -UCN. (F) ROS production from irradiated and non-irradiated anti-EGFR-PEG-TiO 2 -UCNs in PBS and corresponding unmodified TiO 2 -UCNs; * P

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Transmission Electron Microscopy, Fluorescence, Irradiation

    Biodistribution of UCNs measured by ICP-AES analysis, showing uptake of nanoparticles at 4 h, 24 h, 7 d, 28d and 120 d post-injection of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN expressed in terms of percentage of ID per gram of tissue. Data presented are mean (n= 2-3) ± SD.

    Journal: Theranostics

    Article Title: In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

    doi: 10.7150/thno.15088

    Figure Lengend Snippet: Biodistribution of UCNs measured by ICP-AES analysis, showing uptake of nanoparticles at 4 h, 24 h, 7 d, 28d and 120 d post-injection of 50 mg/kg anti-EGFR-PEG-TiO 2 -UCN expressed in terms of percentage of ID per gram of tissue. Data presented are mean (n= 2-3) ± SD.

    Article Snippet: The anti-EGFR affibody conjugated PEG chains (anti-EGFR-PEG-COOH) were subsequently freeze dried, weighed and stored at -20°C until further use.

    Techniques: Injection

    The receptor concentration determined using anti-EGFR affibody RCI is compared to four standard methods used to determine protein concentration: A) pathologist scored ex vivo immunohistochemistry (IHC, r = 0.69, p = 2×10 −5 , m = 0.29 ± 0.06, b = 0.8 ± 0.2), B) ex vivo immunofluorescence ( r = 0.62, p = 5×10 −4 , m = 1.0 ± 0.2, b = 2.1 ± 0.9), C) ex vivo Western blot ( r = 0.35, p = 0.08, m = 0.04 ± 0.03, b = 0.06 ± 0.08), and D) in vitro flow cytometry ( r = 0.43, p = 0.017, m = 9×10 4 ± 3×10 4 , intercept = 4×10 4 ± 1.3×10 5 ). The linear regression (solid line) is displayed as well as the 95% confidence bands (dashed lines) and the 95% prediction bands (dotted lines).

    Journal: Cancer research

    Article Title: Quantitative in vivo immunohistochemistry of epidermal growth factor receptor using a receptor concentration imaging approach

    doi: 10.1158/0008-5472.CAN-14-0141

    Figure Lengend Snippet: The receptor concentration determined using anti-EGFR affibody RCI is compared to four standard methods used to determine protein concentration: A) pathologist scored ex vivo immunohistochemistry (IHC, r = 0.69, p = 2×10 −5 , m = 0.29 ± 0.06, b = 0.8 ± 0.2), B) ex vivo immunofluorescence ( r = 0.62, p = 5×10 −4 , m = 1.0 ± 0.2, b = 2.1 ± 0.9), C) ex vivo Western blot ( r = 0.35, p = 0.08, m = 0.04 ± 0.03, b = 0.06 ± 0.08), and D) in vitro flow cytometry ( r = 0.43, p = 0.017, m = 9×10 4 ± 3×10 4 , intercept = 4×10 4 ± 1.3×10 5 ). The linear regression (solid line) is displayed as well as the 95% confidence bands (dashed lines) and the 95% prediction bands (dotted lines).

    Article Snippet: The targeted anti-EGFR Affibody and untargeted Affibody imaging agent control display similar uptake trends at short time periods after injection ( , columns 1 and 2, respectively) and fluorescence intensity in the tumor region does not reflect EGFR expression levels ( ).

    Techniques: Concentration Assay, Protein Concentration, Ex Vivo, Immunohistochemistry, Immunofluorescence, Western Blot, In Vitro, Flow Cytometry, Cytometry

    Electrophilic affibody improves stability and has high specificity at the mammalian cell surface. COS7 cells expressing ZSPA-TM N6C or N6C F13E were labeled with biotinylated affibody D36C with or without EBA. Affibody binding was detected with streptavidin-Alexa Fluor 555 ( red ), and this staining is overlaid with cyan fluorescent protein co-transfection marker ( blue ) and bright field images. Scale bar , 20 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Electrophilic Affibodies Forming Covalent Bonds to Protein Targets *

    doi: 10.1074/jbc.M109.034322

    Figure Lengend Snippet: Electrophilic affibody improves stability and has high specificity at the mammalian cell surface. COS7 cells expressing ZSPA-TM N6C or N6C F13E were labeled with biotinylated affibody D36C with or without EBA. Affibody binding was detected with streptavidin-Alexa Fluor 555 ( red ), and this staining is overlaid with cyan fluorescent protein co-transfection marker ( blue ) and bright field images. Scale bar , 20 μm.

    Article Snippet: Tetravalent His6 -tagged core streptavidin was purified as described ( ) and fluorescence-labeled with Alexa Fluor 555 succinimidyl ester (Invitrogen) according to manufacturer's instructions, using a 10-fold molar excess of dye.

    Techniques: Expressing, Labeling, Binding Assay, Staining, Cotransfection, Marker

    The tumor-targeting ability of Z HPV16 E7 affitoxin384. The xenograft-bearing mice were generated with cell lines of (A) SiHa, (C) CaSki, (E) HeLa, and (G) A375, respectively. NIR imaging was performed at different time points post injection with DyLight-755-labeled Z HPV16 E7 affitoxin384. DyLight-755-labeled Z HPV16 E7 384 and Z wt affitoxin were used as positive and negative controls, respectively. The tumor/muscle ratios were calculated at 8 h post-injection of indicated agents in xenograft-bearing mice generated with cell lines of (B) SiHa, (D) CaSki, (F) HeLa, and (H) A375, respectively. Data are given as mean ± SD (n=5). ** P

    Journal: Theranostics

    Article Title: A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer

    doi: 10.7150/thno.24607

    Figure Lengend Snippet: The tumor-targeting ability of Z HPV16 E7 affitoxin384. The xenograft-bearing mice were generated with cell lines of (A) SiHa, (C) CaSki, (E) HeLa, and (G) A375, respectively. NIR imaging was performed at different time points post injection with DyLight-755-labeled Z HPV16 E7 affitoxin384. DyLight-755-labeled Z HPV16 E7 384 and Z wt affitoxin were used as positive and negative controls, respectively. The tumor/muscle ratios were calculated at 8 h post-injection of indicated agents in xenograft-bearing mice generated with cell lines of (B) SiHa, (D) CaSki, (F) HeLa, and (H) A375, respectively. Data are given as mean ± SD (n=5). ** P

    Article Snippet: ZHPV16 E7 affitoxin384, ZHPV16 E7 384 (the positive control) and Zwt affitoxin (the negative control) were labeled with a maleimide derivative of DyLight-755 (Thermo Fisher Scientific, USA, 62278) by attaching the dye to the native lysine residues of affibody molecules according to the manufacturer's protocol.

    Techniques: Mouse Assay, Generated, Imaging, Injection, Labeling

    Functional evaluation of the Her2 affibody-SPIO conjugates. Her2/neu-positive and Her2/neu-negative cells were incubated with Her2-SPIO conjugates in the presence and absence of excess free affibody. Free affibody served as a competitive inhibitor to confirm specific binding of the Her2/neu receptor. Relaxivity measurements and T2*-weighted MR images of each cell suspension were acquired.

    Journal: Analytical chemistry

    Article Title: Sortase-Tag Expressed Protein Ligation (STEPL): combining protein purification and site-specific bioconjugation into a single step

    doi: 10.1021/ac402871k

    Figure Lengend Snippet: Functional evaluation of the Her2 affibody-SPIO conjugates. Her2/neu-positive and Her2/neu-negative cells were incubated with Her2-SPIO conjugates in the presence and absence of excess free affibody. Free affibody served as a competitive inhibitor to confirm specific binding of the Her2/neu receptor. Relaxivity measurements and T2*-weighted MR images of each cell suspension were acquired.

    Article Snippet: In this study, the STEPL protocol is optimized, modeled, and used to conjugate the Her2/neu and EGFR-targeting affibody to fluorophores for imaging and/or an azide for subsequent copper-free click chemistry reactions with azadibenzocyclooctyne (ADIBO)-functionalized superparamagnetic iron oxide nanoparticles, demonstrating the system’s flexibility, efficacy, and utility.

    Techniques: Functional Assay, Incubation, Binding Assay

    Functional evaluation of the Her2 affibody-HiLyte Fluor ™ 750 conjugate. (A) Her2/neu positive and negative cells were incubated with Her2/neu-targeted affibodies that were conjugated to HiLyte Fluor ™ 750 (red) using the STEPL system. Cells were also stained with Hoechst 33342 (nuclear stain, blue). (B) In-cell western quantification of HiLyte Fluor ™ 750 fluorescence.

    Journal: Analytical chemistry

    Article Title: Sortase-Tag Expressed Protein Ligation (STEPL): combining protein purification and site-specific bioconjugation into a single step

    doi: 10.1021/ac402871k

    Figure Lengend Snippet: Functional evaluation of the Her2 affibody-HiLyte Fluor ™ 750 conjugate. (A) Her2/neu positive and negative cells were incubated with Her2/neu-targeted affibodies that were conjugated to HiLyte Fluor ™ 750 (red) using the STEPL system. Cells were also stained with Hoechst 33342 (nuclear stain, blue). (B) In-cell western quantification of HiLyte Fluor ™ 750 fluorescence.

    Article Snippet: In this study, the STEPL protocol is optimized, modeled, and used to conjugate the Her2/neu and EGFR-targeting affibody to fluorophores for imaging and/or an azide for subsequent copper-free click chemistry reactions with azadibenzocyclooctyne (ADIBO)-functionalized superparamagnetic iron oxide nanoparticles, demonstrating the system’s flexibility, efficacy, and utility.

    Techniques: Functional Assay, Incubation, Staining, In-Cell ELISA, Fluorescence

    Her2/neu affibody expression and ligation. An SDS-PAGE gel was run with (1) marker, (2) raw lysate of bacterially expressed STEPL-Her2 affibody, and (3) Her2 affibody purified using a 2-fold excess of HiLyte 750-labeled triglycine peptide, 100μM Ca 2+ at 37°C for 6hr. (A) SimplyBlue SafeStain protein stain. (B) HiLyte 750 peptide fluorescence.

    Journal: Analytical chemistry

    Article Title: Sortase-Tag Expressed Protein Ligation (STEPL): combining protein purification and site-specific bioconjugation into a single step

    doi: 10.1021/ac402871k

    Figure Lengend Snippet: Her2/neu affibody expression and ligation. An SDS-PAGE gel was run with (1) marker, (2) raw lysate of bacterially expressed STEPL-Her2 affibody, and (3) Her2 affibody purified using a 2-fold excess of HiLyte 750-labeled triglycine peptide, 100μM Ca 2+ at 37°C for 6hr. (A) SimplyBlue SafeStain protein stain. (B) HiLyte 750 peptide fluorescence.

    Article Snippet: In this study, the STEPL protocol is optimized, modeled, and used to conjugate the Her2/neu and EGFR-targeting affibody to fluorophores for imaging and/or an azide for subsequent copper-free click chemistry reactions with azadibenzocyclooctyne (ADIBO)-functionalized superparamagnetic iron oxide nanoparticles, demonstrating the system’s flexibility, efficacy, and utility.

    Techniques: Expressing, Ligation, SDS Page, Marker, Purification, Labeling, Staining, Fluorescence