imac columns Affibody Search Results


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  • 90
    Thermo Fisher hispur cobalt resin
    Hispur Cobalt Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hispur cobalt resin/product/Thermo Fisher
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    95
    Affibody affibody conjugate
    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR <t>affibody</t> probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).
    Affibody Conjugate, supplied by Affibody, used in various techniques. Bioz Stars score: 95/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affibody conjugate/product/Affibody
    Average 95 stars, based on 138 article reviews
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    96
    Affibody fluorescence imaging probes
    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR <t>affibody</t> probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).
    Fluorescence Imaging Probes, supplied by Affibody, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence imaging probes/product/Affibody
    Average 96 stars, based on 16 article reviews
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    78
    Affibody untargeted affibody imaging agent control
    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR <t>affibody</t> probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).
    Untargeted Affibody Imaging Agent Control, supplied by Affibody, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/untargeted affibody imaging agent control/product/Affibody
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    97
    Affibody anti egfr affibody
    The receptor concentration determined using <t>anti-EGFR</t> <t>affibody</t> RCI is compared to four standard methods used to determine protein concentration: A) pathologist scored ex vivo immunohistochemistry (IHC, r = 0.69, p = 2×10 −5 , m = 0.29 ± 0.06, b = 0.8 ± 0.2), B) ex vivo immunofluorescence ( r = 0.62, p = 5×10 −4 , m = 1.0 ± 0.2, b = 2.1 ± 0.9), C) ex vivo Western blot ( r = 0.35, p = 0.08, m = 0.04 ± 0.03, b = 0.06 ± 0.08), and D) in vitro flow cytometry ( r = 0.43, p = 0.017, m = 9×10 4 ± 3×10 4 , intercept = 4×10 4 ± 1.3×10 5 ). The linear regression (solid line) is displayed as well as the 95% confidence bands (dashed lines) and the 95% prediction bands (dotted lines).
    Anti Egfr Affibody, supplied by Affibody, used in various techniques. Bioz Stars score: 97/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti egfr affibody/product/Affibody
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    99
    GE Healthcare äkta fplc system
    The receptor concentration determined using <t>anti-EGFR</t> <t>affibody</t> RCI is compared to four standard methods used to determine protein concentration: A) pathologist scored ex vivo immunohistochemistry (IHC, r = 0.69, p = 2×10 −5 , m = 0.29 ± 0.06, b = 0.8 ± 0.2), B) ex vivo immunofluorescence ( r = 0.62, p = 5×10 −4 , m = 1.0 ± 0.2, b = 2.1 ± 0.9), C) ex vivo Western blot ( r = 0.35, p = 0.08, m = 0.04 ± 0.03, b = 0.06 ± 0.08), and D) in vitro flow cytometry ( r = 0.43, p = 0.017, m = 9×10 4 ± 3×10 4 , intercept = 4×10 4 ± 1.3×10 5 ). The linear regression (solid line) is displayed as well as the 95% confidence bands (dashed lines) and the 95% prediction bands (dotted lines).
    äkta Fplc System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/äkta fplc system/product/GE Healthcare
    Average 99 stars, based on 3959 article reviews
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    Image Search Results


    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR affibody probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR affibody probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Dissection, Staining, Imaging, Mouse Assay, Injection, Autoradiography, Radioactivity, Fluorescence

    Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with ICG maleimide or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Affibody molecules are shown with randomized positions in the binding site, which are indicated in blue. The molecule was labeled with ICG maleimide or IRDye 700. The figure was adopted from Wallberg H, et al. [ 46 ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Binding Assay, Labeling, Thin Layer Chromatography, Fluorescence

    Near-infrared (NIR) imaging of cell lines by the addition of affibody probes to conditioned medium ( a , b ). SAS cells showed strong fluorescence signals of anti-EGFR affibody imaging probes ( a , left). SAS cells ( a , middle) expressed higher anti-epidermal growth factor receptor (EGFR) levels than MCF-7 cells ( a , right). SK-BR3 cells showed stronger fluorescence signals in anti-HER2 affibody imaging probes than MDA-MB231 cells ( b ). Histological section study ( c ). Anti-HER2 affibody probe was administered to histological sections of lymph nodes from breast cancer patients. Metastatic cancer cells are shown after hematoxylin and eosin (H E) staining ( c , upper row, left). Human epidermal growth factor receptor 2 (HER2) expression was positive in metastatic cancer cells by immunohistochemical staining ( c , upper row, middle). High-intensity NIR signals from the probe identically corresponded with the area of increased HER2 expression ( c , upper row, right). In the HER2-negative metastatic lymph node section ( c , lower row), immunohistochemical staining for HER2 and NIR signals were not visible in metastatic cancer cells ( c , lower row, middle, right). Scale bar in ( a ): 20 μm. Scale bar in ( c ): 200 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Near-infrared (NIR) imaging of cell lines by the addition of affibody probes to conditioned medium ( a , b ). SAS cells showed strong fluorescence signals of anti-EGFR affibody imaging probes ( a , left). SAS cells ( a , middle) expressed higher anti-epidermal growth factor receptor (EGFR) levels than MCF-7 cells ( a , right). SK-BR3 cells showed stronger fluorescence signals in anti-HER2 affibody imaging probes than MDA-MB231 cells ( b ). Histological section study ( c ). Anti-HER2 affibody probe was administered to histological sections of lymph nodes from breast cancer patients. Metastatic cancer cells are shown after hematoxylin and eosin (H E) staining ( c , upper row, left). Human epidermal growth factor receptor 2 (HER2) expression was positive in metastatic cancer cells by immunohistochemical staining ( c , upper row, middle). High-intensity NIR signals from the probe identically corresponded with the area of increased HER2 expression ( c , upper row, right). In the HER2-negative metastatic lymph node section ( c , lower row), immunohistochemical staining for HER2 and NIR signals were not visible in metastatic cancer cells ( c , lower row, middle, right). Scale bar in ( a ): 20 μm. Scale bar in ( c ): 200 μm.

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Fluorescence, Multiple Displacement Amplification, Staining, Expressing, Immunohistochemistry

    Imaging of metastatic cancer cells in lymph nodes. ( a ) In a mouse lymph node metastasis model, an anti-EGFR affibody probe was injected into the mouse tongue 24 h prior to sacrifice and lymph node dissection. Six lymph nodes were excised; three lymph nodes were highly fluorescent, and the remaining three lymph nodes were not fluorescent. Immunohistochemical staining for EGFR was found in fluorescence-positive lymph nodes (lymph node 2 (R), lymph node 3 (R)). EGFR expression was not visible in the nonfluorescent lymph node (lymph node 2 (L)). ( b ) Two lymph nodes, one from each side of the mouse, were dissected 24 h after anti-EGFR affibody probe injection into the tongue. The indocyanine green (ICG) fluorescence signal was obvious and corresponded to the immunohistochemically stained EGFR expression in the lymph nodes (red circles and arrows). The panel on the right is a magnified view of the two lymph nodes in the left panel of the NIR images.

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Imaging of metastatic cancer cells in lymph nodes. ( a ) In a mouse lymph node metastasis model, an anti-EGFR affibody probe was injected into the mouse tongue 24 h prior to sacrifice and lymph node dissection. Six lymph nodes were excised; three lymph nodes were highly fluorescent, and the remaining three lymph nodes were not fluorescent. Immunohistochemical staining for EGFR was found in fluorescence-positive lymph nodes (lymph node 2 (R), lymph node 3 (R)). EGFR expression was not visible in the nonfluorescent lymph node (lymph node 2 (L)). ( b ) Two lymph nodes, one from each side of the mouse, were dissected 24 h after anti-EGFR affibody probe injection into the tongue. The indocyanine green (ICG) fluorescence signal was obvious and corresponded to the immunohistochemically stained EGFR expression in the lymph nodes (red circles and arrows). The panel on the right is a magnified view of the two lymph nodes in the left panel of the NIR images.

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Injection, Dissection, Immunohistochemistry, Staining, Fluorescence, Expressing

    Dynamic imaging study. ( a ) NIR images showed changes in the signal from the anti-EGFR affibody probe in the lymph nodes of a normal control mouse (left). The fluorescence signal intensity was examined at 0.5, 1, 2, 3, 4, 6, and 24 h after the tongue injection in two mice (total four lymph nodes). The peak intensity was observed at one and two hours after the injection (right). The error bar shows the standard deviation. ( b ) The left panels are images of metastatic and nonmetastatic lymph nodes. Weak, almost equal NIR signal intensity was found in two nonmetastatic lymph nodes at 0.5 and 1 h post-injection. The signal intensity almost disappeared at 24 h after the injection (top, left). A high signal intensity remained in the metastatic lymph node (arrow) at 24 h after injection (bottom, left). The time-dependent NIR signal intensity of the anti-EGFR affibody probe is represented as a percentage of the initial signal intensity (30 min). The signal intensity ratio was greater for metastatic lymph nodes than nonmetastatic lymph nodes at 1, 2, 3, and 6 h after the injection (right panel). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Dynamic imaging study. ( a ) NIR images showed changes in the signal from the anti-EGFR affibody probe in the lymph nodes of a normal control mouse (left). The fluorescence signal intensity was examined at 0.5, 1, 2, 3, 4, 6, and 24 h after the tongue injection in two mice (total four lymph nodes). The peak intensity was observed at one and two hours after the injection (right). The error bar shows the standard deviation. ( b ) The left panels are images of metastatic and nonmetastatic lymph nodes. Weak, almost equal NIR signal intensity was found in two nonmetastatic lymph nodes at 0.5 and 1 h post-injection. The signal intensity almost disappeared at 24 h after the injection (top, left). A high signal intensity remained in the metastatic lymph node (arrow) at 24 h after injection (bottom, left). The time-dependent NIR signal intensity of the anti-EGFR affibody probe is represented as a percentage of the initial signal intensity (30 min). The signal intensity ratio was greater for metastatic lymph nodes than nonmetastatic lymph nodes at 1, 2, 3, and 6 h after the injection (right panel). * p

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Fluorescence, Injection, Mouse Assay, Standard Deviation

    ( a ) Image of a custom-built illuminator (light emitting diode (LED) emission: peak wavelength, 690 nm). Image of SAS xenograft tumor in the back of a mouse after anti-EGFR affibody photoimmunotherapy (PIT). The image was captured one hour after the probe was injected into the right tumor. The contralateral xenograft tumor served as a control (right). ( b ) The CCK-8 assay showed that the combination of the EGFR affibody IR700 probe and NIR irradiation decreased the survival rate of SAS cells rather than exposure to NIR or the EGFR affibody IR700 probe alone ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: ( a ) Image of a custom-built illuminator (light emitting diode (LED) emission: peak wavelength, 690 nm). Image of SAS xenograft tumor in the back of a mouse after anti-EGFR affibody photoimmunotherapy (PIT). The image was captured one hour after the probe was injected into the right tumor. The contralateral xenograft tumor served as a control (right). ( b ) The CCK-8 assay showed that the combination of the EGFR affibody IR700 probe and NIR irradiation decreased the survival rate of SAS cells rather than exposure to NIR or the EGFR affibody IR700 probe alone ( p

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Injection, CCK-8 Assay, Irradiation

    Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR affibody probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Neck lymph node in normal mouse ( a ). The arrows indicate neck lymph nodes in dissection. H E staining showed lymph nodes in the excised cervical tissue ( a ). Two-phase combined imaging of SLNs in normal mice ( b ). 99m Tc-Phytate and anti-EGFR affibody probe were injected into the tongue of mice. Two excised neck specimens with lymph nodes from two mice are shown ( b , left). Autoradiography showed that 99m Tc radioactivity accumulated in the lymph nodes of each neck specimen and one solitary lymph node in the corresponding tissues of the left panel ( b , middle). NIR fluorescence was localized in the lymph nodes after anti-EGFR affibody probe injection into the tongue ( b , right).

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Dissection, Staining, Imaging, Mouse Assay, Injection, Autoradiography, Radioactivity, Fluorescence

    ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: ] ( a ). Thin-layer chromatography (TLC) showed that the fluorescence droplet spots of ICG-conjugated anti-EGFR, anti-HER2 affibody, and IR700-conjugated anti-EGFR affibody (2 µL) stayed at the starting point; in contrast, free ICG and free IR700 ran on the slides ( b ).

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Thin Layer Chromatography, Fluorescence

    Near-infrared (NIR) imaging of cell lines by the addition of affibody probes to conditioned medium ( a , b ). SAS cells showed strong fluorescence signals of anti-EGFR affibody imaging probes ( a , left). SAS cells ( a , middle) expressed higher anti-epidermal growth factor receptor (EGFR) levels than MCF-7 cells ( a , right). SK-BR3 cells showed stronger fluorescence signals in anti-HER2 affibody imaging probes than MDA-MB231 cells ( b ). Histological section study ( c ). Anti-HER2 affibody probe was administered to histological sections of lymph nodes from breast cancer patients. Metastatic cancer cells are shown after hematoxylin and eosin (H E) staining ( c , upper row, left). Human epidermal growth factor receptor 2 (HER2) expression was positive in metastatic cancer cells by immunohistochemical staining ( c , upper row, middle). High-intensity NIR signals from the probe identically corresponded with the area of increased HER2 expression ( c , upper row, right). In the HER2-negative metastatic lymph node section ( c , lower row), immunohistochemical staining for HER2 and NIR signals were not visible in metastatic cancer cells ( c , lower row, middle, right). Scale bar in ( a ): 20 μm. Scale bar in ( c ): 200 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Near-infrared (NIR) imaging of cell lines by the addition of affibody probes to conditioned medium ( a , b ). SAS cells showed strong fluorescence signals of anti-EGFR affibody imaging probes ( a , left). SAS cells ( a , middle) expressed higher anti-epidermal growth factor receptor (EGFR) levels than MCF-7 cells ( a , right). SK-BR3 cells showed stronger fluorescence signals in anti-HER2 affibody imaging probes than MDA-MB231 cells ( b ). Histological section study ( c ). Anti-HER2 affibody probe was administered to histological sections of lymph nodes from breast cancer patients. Metastatic cancer cells are shown after hematoxylin and eosin (H E) staining ( c , upper row, left). Human epidermal growth factor receptor 2 (HER2) expression was positive in metastatic cancer cells by immunohistochemical staining ( c , upper row, middle). High-intensity NIR signals from the probe identically corresponded with the area of increased HER2 expression ( c , upper row, right). In the HER2-negative metastatic lymph node section ( c , lower row), immunohistochemical staining for HER2 and NIR signals were not visible in metastatic cancer cells ( c , lower row, middle, right). Scale bar in ( a ): 20 μm. Scale bar in ( c ): 200 μm.

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Fluorescence, Multiple Displacement Amplification, Staining, Expressing, Immunohistochemistry

    Imaging of metastatic cancer cells in lymph nodes. ( a ) In a mouse lymph node metastasis model, an anti-EGFR affibody probe was injected into the mouse tongue 24 h prior to sacrifice and lymph node dissection. Six lymph nodes were excised; three lymph nodes were highly fluorescent, and the remaining three lymph nodes were not fluorescent. Immunohistochemical staining for EGFR was found in fluorescence-positive lymph nodes (lymph node 2 (R), lymph node 3 (R)). EGFR expression was not visible in the nonfluorescent lymph node (lymph node 2 (L)). ( b ) Two lymph nodes, one from each side of the mouse, were dissected 24 h after anti-EGFR affibody probe injection into the tongue. The indocyanine green (ICG) fluorescence signal was obvious and corresponded to the immunohistochemically stained EGFR expression in the lymph nodes (red circles and arrows). The panel on the right is a magnified view of the two lymph nodes in the left panel of the NIR images.

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Imaging of metastatic cancer cells in lymph nodes. ( a ) In a mouse lymph node metastasis model, an anti-EGFR affibody probe was injected into the mouse tongue 24 h prior to sacrifice and lymph node dissection. Six lymph nodes were excised; three lymph nodes were highly fluorescent, and the remaining three lymph nodes were not fluorescent. Immunohistochemical staining for EGFR was found in fluorescence-positive lymph nodes (lymph node 2 (R), lymph node 3 (R)). EGFR expression was not visible in the nonfluorescent lymph node (lymph node 2 (L)). ( b ) Two lymph nodes, one from each side of the mouse, were dissected 24 h after anti-EGFR affibody probe injection into the tongue. The indocyanine green (ICG) fluorescence signal was obvious and corresponded to the immunohistochemically stained EGFR expression in the lymph nodes (red circles and arrows). The panel on the right is a magnified view of the two lymph nodes in the left panel of the NIR images.

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Injection, Dissection, Immunohistochemistry, Staining, Fluorescence, Expressing

    Dynamic imaging study. ( a ) NIR images showed changes in the signal from the anti-EGFR affibody probe in the lymph nodes of a normal control mouse (left). The fluorescence signal intensity was examined at 0.5, 1, 2, 3, 4, 6, and 24 h after the tongue injection in two mice (total four lymph nodes). The peak intensity was observed at one and two hours after the injection (right). The error bar shows the standard deviation. ( b ) The left panels are images of metastatic and nonmetastatic lymph nodes. Weak, almost equal NIR signal intensity was found in two nonmetastatic lymph nodes at 0.5 and 1 h post-injection. The signal intensity almost disappeared at 24 h after the injection (top, left). A high signal intensity remained in the metastatic lymph node (arrow) at 24 h after injection (bottom, left). The time-dependent NIR signal intensity of the anti-EGFR affibody probe is represented as a percentage of the initial signal intensity (30 min). The signal intensity ratio was greater for metastatic lymph nodes than nonmetastatic lymph nodes at 1, 2, 3, and 6 h after the injection (right panel). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: Dynamic imaging study. ( a ) NIR images showed changes in the signal from the anti-EGFR affibody probe in the lymph nodes of a normal control mouse (left). The fluorescence signal intensity was examined at 0.5, 1, 2, 3, 4, 6, and 24 h after the tongue injection in two mice (total four lymph nodes). The peak intensity was observed at one and two hours after the injection (right). The error bar shows the standard deviation. ( b ) The left panels are images of metastatic and nonmetastatic lymph nodes. Weak, almost equal NIR signal intensity was found in two nonmetastatic lymph nodes at 0.5 and 1 h post-injection. The signal intensity almost disappeared at 24 h after the injection (top, left). A high signal intensity remained in the metastatic lymph node (arrow) at 24 h after injection (bottom, left). The time-dependent NIR signal intensity of the anti-EGFR affibody probe is represented as a percentage of the initial signal intensity (30 min). The signal intensity ratio was greater for metastatic lymph nodes than nonmetastatic lymph nodes at 1, 2, 3, and 6 h after the injection (right panel). * p

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Imaging, Fluorescence, Injection, Mouse Assay, Standard Deviation

    ( a ) Image of a custom-built illuminator (light emitting diode (LED) emission: peak wavelength, 690 nm). Image of SAS xenograft tumor in the back of a mouse after anti-EGFR affibody photoimmunotherapy (PIT). The image was captured one hour after the probe was injected into the right tumor. The contralateral xenograft tumor served as a control (right). ( b ) The CCK-8 assay showed that the combination of the EGFR affibody IR700 probe and NIR irradiation decreased the survival rate of SAS cells rather than exposure to NIR or the EGFR affibody IR700 probe alone ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach

    doi: 10.3390/ijms20020427

    Figure Lengend Snippet: ( a ) Image of a custom-built illuminator (light emitting diode (LED) emission: peak wavelength, 690 nm). Image of SAS xenograft tumor in the back of a mouse after anti-EGFR affibody photoimmunotherapy (PIT). The image was captured one hour after the probe was injected into the right tumor. The contralateral xenograft tumor served as a control (right). ( b ) The CCK-8 assay showed that the combination of the EGFR affibody IR700 probe and NIR irradiation decreased the survival rate of SAS cells rather than exposure to NIR or the EGFR affibody IR700 probe alone ( p

    Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).

    Techniques: Injection, CCK-8 Assay, Irradiation

    The receptor concentration determined using anti-EGFR affibody RCI is compared to four standard methods used to determine protein concentration: A) pathologist scored ex vivo immunohistochemistry (IHC, r = 0.69, p = 2×10 −5 , m = 0.29 ± 0.06, b = 0.8 ± 0.2), B) ex vivo immunofluorescence ( r = 0.62, p = 5×10 −4 , m = 1.0 ± 0.2, b = 2.1 ± 0.9), C) ex vivo Western blot ( r = 0.35, p = 0.08, m = 0.04 ± 0.03, b = 0.06 ± 0.08), and D) in vitro flow cytometry ( r = 0.43, p = 0.017, m = 9×10 4 ± 3×10 4 , intercept = 4×10 4 ± 1.3×10 5 ). The linear regression (solid line) is displayed as well as the 95% confidence bands (dashed lines) and the 95% prediction bands (dotted lines).

    Journal: Cancer research

    Article Title: Quantitative in vivo immunohistochemistry of epidermal growth factor receptor using a receptor concentration imaging approach

    doi: 10.1158/0008-5472.CAN-14-0141

    Figure Lengend Snippet: The receptor concentration determined using anti-EGFR affibody RCI is compared to four standard methods used to determine protein concentration: A) pathologist scored ex vivo immunohistochemistry (IHC, r = 0.69, p = 2×10 −5 , m = 0.29 ± 0.06, b = 0.8 ± 0.2), B) ex vivo immunofluorescence ( r = 0.62, p = 5×10 −4 , m = 1.0 ± 0.2, b = 2.1 ± 0.9), C) ex vivo Western blot ( r = 0.35, p = 0.08, m = 0.04 ± 0.03, b = 0.06 ± 0.08), and D) in vitro flow cytometry ( r = 0.43, p = 0.017, m = 9×10 4 ± 3×10 4 , intercept = 4×10 4 ± 1.3×10 5 ). The linear regression (solid line) is displayed as well as the 95% confidence bands (dashed lines) and the 95% prediction bands (dotted lines).

    Article Snippet: The targeted anti-EGFR Affibody and untargeted Affibody imaging agent control display similar uptake trends at short time periods after injection ( , columns 1 and 2, respectively) and fluorescence intensity in the tumor region does not reflect EGFR expression levels ( ).

    Techniques: Concentration Assay, Protein Concentration, Ex Vivo, Immunohistochemistry, Immunofluorescence, Western Blot, In Vitro, Flow Cytometry, Cytometry