Journal: International Journal of Molecular Sciences
Article Title: Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach
Figure Lengend Snippet: Near-infrared (NIR) imaging of cell lines by the addition of affibody probes to conditioned medium ( a , b ). SAS cells showed strong fluorescence signals of anti-EGFR affibody imaging probes ( a , left). SAS cells ( a , middle) expressed higher anti-epidermal growth factor receptor (EGFR) levels than MCF-7 cells ( a , right). SK-BR3 cells showed stronger fluorescence signals in anti-HER2 affibody imaging probes than MDA-MB231 cells ( b ). Histological section study ( c ). Anti-HER2 affibody probe was administered to histological sections of lymph nodes from breast cancer patients. Metastatic cancer cells are shown after hematoxylin and eosin (H E) staining ( c , upper row, left). Human epidermal growth factor receptor 2 (HER2) expression was positive in metastatic cancer cells by immunohistochemical staining ( c , upper row, middle). High-intensity NIR signals from the probe identically corresponded with the area of increased HER2 expression ( c , upper row, right). In the HER2-negative metastatic lymph node section ( c , lower row), immunohistochemical staining for HER2 and NIR signals were not visible in metastatic cancer cells ( c , lower row, middle, right). Scale bar in ( a ): 20 μm. Scale bar in ( c ): 200 μm.
Article Snippet: This solution was added to the affibody conjugate and incubated at 37 °C for 60 min, followed by desalting using a protein desalting spin column (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 1500× g for 2 min to produce the affibody fluorescence imaging probes (i.e., affibody probe).
Techniques: Imaging, Fluorescence, Multiple Displacement Amplification, Staining, Expressing, Immunohistochemistry