illustra genomiphi v2 dna amplification kit Ge Healthcare Search Results


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    GE Healthcare illustra genomiphi v2 dna amplification kit ge healthcare
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Illustra Genomiphi V2 Dna Amplification Kit Ge Healthcare, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 dna amplification kit ge healthcare/product/GE Healthcare
    Average 85 stars, based on 190 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 dna amplification kit ge healthcare - by Bioz Stars, 2020-07
    85/100 stars
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    92
    GE Healthcare phi29 amplified dna
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Phi29 Amplified Dna, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phi29 amplified dna/product/GE Healthcare
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    phi29 amplified dna - by Bioz Stars, 2020-07
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    85
    GE Healthcare illustra genomiphi v2 dna kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Illustra Genomiphi V2 Dna Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 dna kit/product/GE Healthcare
    Average 85 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 dna kit - by Bioz Stars, 2020-07
    85/100 stars
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    85
    GE Healthcare part of the illustra genomiphi dna amplification kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Part Of The Illustra Genomiphi Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/part of the illustra genomiphi dna amplification kit/product/GE Healthcare
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    part of the illustra genomiphi dna amplification kit - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    85
    GE Healthcare wga reactions illustra genomiphi v2 dna amplification kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Wga Reactions Illustra Genomiphi V2 Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wga reactions illustra genomiphi v2 dna amplification kit/product/GE Healthcare
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    wga reactions illustra genomiphi v2 dna amplification kit - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    86
    GE Healthcare whole genome amplification illustra genomiphi v2 dna amplification kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Whole Genome Amplification Illustra Genomiphi V2 Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole genome amplification illustra genomiphi v2 dna amplification kit/product/GE Healthcare
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    whole genome amplification illustra genomiphi v2 dna amplification kit - by Bioz Stars, 2020-07
    86/100 stars
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    88
    GE Healthcare illustra genomiphi v2 dna multiple displacement amplification mda kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Illustra Genomiphi V2 Dna Multiple Displacement Amplification Mda Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 dna multiple displacement amplification mda kit/product/GE Healthcare
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 dna multiple displacement amplification mda kit - by Bioz Stars, 2020-07
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    88
    GE Healthcare illustra genomiphi v2 kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Illustra Genomiphi V2 Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 kit/product/GE Healthcare
    Average 88 stars, based on 214 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 kit - by Bioz Stars, 2020-07
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      Buy from Supplier

    91
    GE Healthcare genomiphi kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Genomiphi Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomiphi kit/product/GE Healthcare
    Average 91 stars, based on 384 article reviews
    Price from $9.99 to $1999.99
    genomiphi kit - by Bioz Stars, 2020-07
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    93
    GE Healthcare reaction buffer
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Reaction Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reaction buffer/product/GE Healthcare
    Average 93 stars, based on 1339 article reviews
    Price from $9.99 to $1999.99
    reaction buffer - by Bioz Stars, 2020-07
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    85
    GE Healthcare illustra genomiphi v2 d amplification kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Illustra Genomiphi V2 D Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 d amplification kit/product/GE Healthcare
    Average 85 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 d amplification kit - by Bioz Stars, 2020-07
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    88
    GE Healthcare mda method
    Library quality controls. ( A ) HES3 Human embryonic stem cells were grown and prepared as described ( 9 ). Total RNA was prepared by the Trizol isolation method. A smear of RNA with two bright bands corresponding to the 28S and 18S rRNA was obtained. The ladder used in all panels is Generuler 1 Kb (Fermentas) ( http://www.fermentas.com/catalog/electrophoresis/images/generuler031123.jpg ). ( B ) The mRNA prepared by the use of the μMACS mRNA isolation kit on total RNA showed no bright bands corresponding to the rRNA. ( C ) A flcDNA library was prepared by the Captrapper method, which had a titer of 4.6 × 10 6 cfu. Colony PCR quality control of the library was performed. An empty vector will produce a PCR product of size 260 bp (corresponds to the first band of the ladder); insert sizes were therefore calculated by subtracting off the size of the empty vector. Colony PCR therefore showed a range of insert sizes from 250 to 2000 bp (corresponds to the second to seventh bands of the ladder). This is expected, as a flcDNA library is expected to give a range of different-sized inserts, with no single dominant size. Given that the library was of good quality, as can be seen from the colony PCR, the library was used to prepare two libraries: A single-PET library by the classic method, and a single-PET library by the <t>Selection-MDA</t> method. ( D ) A single-PET library was prepared from the full-length library as per the classic bacterial propagation method. Colony PCR quality control of this library showed a single predominant fixed size of 300 bp in many colonies, which is expected, as single-PET plasmids all have a fixed size of 2800 bp, and hence upon PCR, will give a band of 300 bp. Certain clones do not show this fixed size, which could be the result of the incorporation of foreign <t>DNA,</t> or other factors. Colony PCR quality control showed an insert ratio of 75% based on the number of wells that had PCR products of the correct size (300 bp).
    Mda Method, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda method/product/GE Healthcare
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    93
    GE Healthcare geh mda
    Gel analysis of WGA products from TE biopsies. M1, DNA molecular size marker; M2, DNA molecular size marker; Lane 1, MALBAC; Lane 2, Sureplex; Lane 3, <t>GEH-MDA;</t> Lane 4, RG-MDA.
    Geh Mda, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geh mda/product/GE Healthcare
    Average 93 stars, based on 18 article reviews
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    Image Search Results


    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Journal: GigaScience

    Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

    doi: 10.1186/s13742-015-0068-3

    Figure Lengend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Article Snippet: The kits used were Qiagen REPLI-g Mini Kit (MDA-1), Qiagen REPLI-g Single Cell Kit (MDA-2), GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3), GenomePlex® Single Cell WGA Kit (DOP-1), Silicon Biosystem Ampli1™ WGA Kit (DOP-2), NEB Single Cell WGA Kit (DOP-3), and Yikon Genomics Single Cell WGA Kit (MALBAC).

    Techniques: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

    Library quality controls. ( A ) HES3 Human embryonic stem cells were grown and prepared as described ( 9 ). Total RNA was prepared by the Trizol isolation method. A smear of RNA with two bright bands corresponding to the 28S and 18S rRNA was obtained. The ladder used in all panels is Generuler 1 Kb (Fermentas) ( http://www.fermentas.com/catalog/electrophoresis/images/generuler031123.jpg ). ( B ) The mRNA prepared by the use of the μMACS mRNA isolation kit on total RNA showed no bright bands corresponding to the rRNA. ( C ) A flcDNA library was prepared by the Captrapper method, which had a titer of 4.6 × 10 6 cfu. Colony PCR quality control of the library was performed. An empty vector will produce a PCR product of size 260 bp (corresponds to the first band of the ladder); insert sizes were therefore calculated by subtracting off the size of the empty vector. Colony PCR therefore showed a range of insert sizes from 250 to 2000 bp (corresponds to the second to seventh bands of the ladder). This is expected, as a flcDNA library is expected to give a range of different-sized inserts, with no single dominant size. Given that the library was of good quality, as can be seen from the colony PCR, the library was used to prepare two libraries: A single-PET library by the classic method, and a single-PET library by the Selection-MDA method. ( D ) A single-PET library was prepared from the full-length library as per the classic bacterial propagation method. Colony PCR quality control of this library showed a single predominant fixed size of 300 bp in many colonies, which is expected, as single-PET plasmids all have a fixed size of 2800 bp, and hence upon PCR, will give a band of 300 bp. Certain clones do not show this fixed size, which could be the result of the incorporation of foreign DNA, or other factors. Colony PCR quality control showed an insert ratio of 75% based on the number of wells that had PCR products of the correct size (300 bp).

    Journal: Nucleic Acids Research

    Article Title: The use of multiple displacement amplification to amplify complex DNA libraries

    doi: 10.1093/nar/gkn074

    Figure Lengend Snippet: Library quality controls. ( A ) HES3 Human embryonic stem cells were grown and prepared as described ( 9 ). Total RNA was prepared by the Trizol isolation method. A smear of RNA with two bright bands corresponding to the 28S and 18S rRNA was obtained. The ladder used in all panels is Generuler 1 Kb (Fermentas) ( http://www.fermentas.com/catalog/electrophoresis/images/generuler031123.jpg ). ( B ) The mRNA prepared by the use of the μMACS mRNA isolation kit on total RNA showed no bright bands corresponding to the rRNA. ( C ) A flcDNA library was prepared by the Captrapper method, which had a titer of 4.6 × 10 6 cfu. Colony PCR quality control of the library was performed. An empty vector will produce a PCR product of size 260 bp (corresponds to the first band of the ladder); insert sizes were therefore calculated by subtracting off the size of the empty vector. Colony PCR therefore showed a range of insert sizes from 250 to 2000 bp (corresponds to the second to seventh bands of the ladder). This is expected, as a flcDNA library is expected to give a range of different-sized inserts, with no single dominant size. Given that the library was of good quality, as can be seen from the colony PCR, the library was used to prepare two libraries: A single-PET library by the classic method, and a single-PET library by the Selection-MDA method. ( D ) A single-PET library was prepared from the full-length library as per the classic bacterial propagation method. Colony PCR quality control of this library showed a single predominant fixed size of 300 bp in many colonies, which is expected, as single-PET plasmids all have a fixed size of 2800 bp, and hence upon PCR, will give a band of 300 bp. Certain clones do not show this fixed size, which could be the result of the incorporation of foreign DNA, or other factors. Colony PCR quality control showed an insert ratio of 75% based on the number of wells that had PCR products of the correct size (300 bp).

    Article Snippet: With recent improvements in the MDA method (for example, the Illustra Genomiphi V2 DNA Amplification kit from GE Healthcare), further time savings could be possible.

    Techniques: Isolation, Electrophoresis, Polymerase Chain Reaction, Plasmid Preparation, Positron Emission Tomography, Selection, Multiple Displacement Amplification, Clone Assay

    Gel analysis of WGA products from TE biopsies. M1, DNA molecular size marker; M2, DNA molecular size marker; Lane 1, MALBAC; Lane 2, Sureplex; Lane 3, GEH-MDA; Lane 4, RG-MDA.

    Journal: PLoS ONE

    Article Title: Novel Double Factor PGT strategy analyzing blastocyst stage embryos in a single NGS procedure

    doi: 10.1371/journal.pone.0205692

    Figure Lengend Snippet: Gel analysis of WGA products from TE biopsies. M1, DNA molecular size marker; M2, DNA molecular size marker; Lane 1, MALBAC; Lane 2, Sureplex; Lane 3, GEH-MDA; Lane 4, RG-MDA.

    Article Snippet: Pools containing 8 cells were amplified by using the following WGA kits: Sureplex (SurePlex DNA Amplification System, PR-40-415101-00, BlueGnome), MALBAC (One Single Cell WGA kit, Yikon Genomics; YK001B), GEH-MDA (illustra GenomiPhi V2 DNA Amplification, GE Healthcare; Kit Cat No./ID 25660030) and RG-MDA (REPLI-g Single Cell kit, Qiagen; Cat No./ID:150343).

    Techniques: Whole Genome Amplification, Marker, Multiple Annealing and Looping–Based Amplification Cycles, Multiple Displacement Amplification