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  • 84
    Illumina Inc illumina miseq illumine
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq Illumine, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 1556 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq illumine/product/Illumina Inc
    Average 84 stars, based on 1556 article reviews
    Price from $9.99 to $1999.99
    illumina miseq illumine - by Bioz Stars, 2020-08
    84/100 stars
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    91
    GATC Biotech illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 91/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/GATC Biotech
    Average 91 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
    91/100 stars
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    93
    Thermo Fisher illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/Thermo Fisher
    Average 93 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    93
    ChunLab illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by ChunLab, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/ChunLab
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    91
    ProteinCT illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by ProteinCT, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/ProteinCT
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
    91/100 stars
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    92
    Roche illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/Roche
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    92
    Sangon Biotech illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/Sangon Biotech
    Average 92 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    92
    Genomed GmbH illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/Genomed GmbH
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    91
    SciGenom illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by SciGenom, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/SciGenom
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    92
    3M Co illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by 3M Co, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/3M Co
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
    92/100 stars
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    90
    Novogene illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/Novogene
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    90
    Pacific Biosciences illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/Pacific Biosciences
    Average 90 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
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    91
    Macrogen illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by Macrogen, used in various techniques. Bioz Stars score: 91/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/Macrogen
    Average 91 stars, based on 30 article reviews
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    illumina miseq - by Bioz Stars, 2020-08
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    91
    SeqWright illumina miseq
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq, supplied by SeqWright, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq/product/SeqWright
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    illumina miseq - by Bioz Stars, 2020-08
    91/100 stars
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    90
    Illumina Inc illumina miseq cartridge
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
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    Sangon Biotech illumina miseq 2000
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq 2000, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq 300, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc illumina miseq instrumentation
    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, <t>Illumina</t> adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina <t>MiSeq</t> (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .
    Illumina Miseq Instrumentation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, Illumina adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina MiSeq (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .

    Journal: bioRxiv

    Article Title: Efficient Retroelement-Mediated DNA Writing in Bacteria

    doi: 10.1101/2020.02.21.958983

    Figure Lengend Snippet: Strategy used to deplete unedited memory registers from dual-register amplicons and the frequency of cell-cell interactions recovered by high-throughput sequencing in the connectome mapping experiment. (A) Using restriction digestion as an alternative strategy to remove unedited registers from the PCR amplified amplicons instead of allele-specific PCR. Genomic DNA samples were purified from the parental recipient cells (MG1655 Δ recJ Δ xonA galK OFF ), as well as cultures obtained after conjugation (transconjugants) in the experiment described in Fig. 4C . The galK locus was PCR amplified from the purified genomic DNA samples and run on a 6% TBE gel before and after digestion with ClaI and AgeI enzymes (which cut unedited Register 1 and Register 2, respectively) and stained by SYBR gold. The galK amplicon obtained from the parental sample was completely digested after enzymatic digestion. In contrast, the galK amplicon obtained from the transconjugant sample was not completely digested by ClaI and AgeI. The undigested band, corresponding to edited registers, comprised ∼3.9% of the signal in this lane (measured by densitometry). (B) This band was subsequently excised, purified and Sanger-sequenced. Drops in the quality of sequencing in Register 1 and 2 indicate the presence of mixed DNA populations containing variations in these two regions in these samples. Subsequently, Illumina adaptors and barcodes were added to this undigested amplicon using an additional round of PCR and the obtained amplicon was sequenced by Illumina MiSeq (see Methods). (C) Number of unique variants (interactions) per million reads obtained from sequencing the two target registers in the genomes of recipient cells after conjugation with donor cells, as well as two randomly selected non-targeted regions within the galK amplicon (used as a negative control and to assess the rate of false-positives), for the experiment shown in Fig. 4C .

    Article Snippet: High-throughput Sequencing Allele frequencies of the HiSCRIBE target sites were measured by sequencing amplicons obtained from corresponding genomic sites using Illumina MiSeq.

    Techniques: Next-Generation Sequencing, Polymerase Chain Reaction, Amplification, Purification, Conjugation Assay, Staining, Sequencing, Negative Control

    High-throughput workflow for the preparation of plasmid DNA libraries for NGS. Plasmid DNA samples are isolated from bacteria cells using a high-throughput plasmid isolation method on the CyBio ® FeliX robot. All steps performed using the FeliX platform are highlighted with a grey outline. The isolated plasmid samples are tested for the presence of genomic DNA (gDNA), prior to library preparation, using the Labcyte Echo ® . All steps performed using the Labcyte Echo ® are highlighted with a shaded grey box. If samples are free from gDNA, they are diluted to 0.4 ng/μl in H 2 O. If gDNA is detected, the samples are sonicated prior to re-testing in the gDNA QC assay. Using reagents from the Nextera XT kit, a tagmentation reaction is performed on all samples under the optimised conditions, followed by neutralization of the reaction. Unique combinations of index primers are added to all samples via 12 PCR cycles, followed by magnetic bead purification of the PCR products. The concentration of the purified dsDNA is then determined using the PicoGreen ® reagent assay and the libraries are pooled to give a final concentration of 4–10 nM, in a minimum volume of 15 μl. The average fragment size of the pooled libraries is measured using the Fragment Analyzer before being sequenced on the Illumina ® MiSeq system.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

    doi: 10.1016/j.synbio.2019.01.002

    Figure Lengend Snippet: High-throughput workflow for the preparation of plasmid DNA libraries for NGS. Plasmid DNA samples are isolated from bacteria cells using a high-throughput plasmid isolation method on the CyBio ® FeliX robot. All steps performed using the FeliX platform are highlighted with a grey outline. The isolated plasmid samples are tested for the presence of genomic DNA (gDNA), prior to library preparation, using the Labcyte Echo ® . All steps performed using the Labcyte Echo ® are highlighted with a shaded grey box. If samples are free from gDNA, they are diluted to 0.4 ng/μl in H 2 O. If gDNA is detected, the samples are sonicated prior to re-testing in the gDNA QC assay. Using reagents from the Nextera XT kit, a tagmentation reaction is performed on all samples under the optimised conditions, followed by neutralization of the reaction. Unique combinations of index primers are added to all samples via 12 PCR cycles, followed by magnetic bead purification of the PCR products. The concentration of the purified dsDNA is then determined using the PicoGreen ® reagent assay and the libraries are pooled to give a final concentration of 4–10 nM, in a minimum volume of 15 μl. The average fragment size of the pooled libraries is measured using the Fragment Analyzer before being sequenced on the Illumina ® MiSeq system.

    Article Snippet: The library was sequenced using the Illumina® MiSeq system, with the MiSeq Micro reagent kit v2 (2 × 150 bp reads; Illumina, Inc., USA).

    Techniques: High Throughput Screening Assay, Plasmid Preparation, Next-Generation Sequencing, Isolation, Sonication, Neutralization, Polymerase Chain Reaction, Purification, Concentration Assay

    Next generation sequencing of plasmid DNA libraries, prepared using a miniaturised method with the Nextera XT library preparation kit. 96 plasmid DNA libraries were prepared for NGS with the Nextera XT library preparation kit, using optimised conditions (12.5 min incubation, 50 nl sample, 1.8x magnetic bead solution). ( A ) After purification, the samples were quantified in the PicoGreen ® dsDNA quantification assay. The data show that 75/92 samples have a concentration within the desired range (0.5–5 ng/μl), with an average concentration of 1.1 ng/μl. These samples were pooled, with a final concentration of 6.64 nM and run on the Fragment Analyzer ( B ) and ( C ). All fragments in the pooled libraries are of the desired size (200–400 bp). ( D ) The pooled library was sequenced on the Illumina ® MiSeq system (2 × 150 method). The mean sequence quality (Phred) scores are plotted for each sample. For all samples, the sequence quality (Phred) score was > 30 for more than 85% of the base pairs, indicating that all samples passed the QC criteria.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

    doi: 10.1016/j.synbio.2019.01.002

    Figure Lengend Snippet: Next generation sequencing of plasmid DNA libraries, prepared using a miniaturised method with the Nextera XT library preparation kit. 96 plasmid DNA libraries were prepared for NGS with the Nextera XT library preparation kit, using optimised conditions (12.5 min incubation, 50 nl sample, 1.8x magnetic bead solution). ( A ) After purification, the samples were quantified in the PicoGreen ® dsDNA quantification assay. The data show that 75/92 samples have a concentration within the desired range (0.5–5 ng/μl), with an average concentration of 1.1 ng/μl. These samples were pooled, with a final concentration of 6.64 nM and run on the Fragment Analyzer ( B ) and ( C ). All fragments in the pooled libraries are of the desired size (200–400 bp). ( D ) The pooled library was sequenced on the Illumina ® MiSeq system (2 × 150 method). The mean sequence quality (Phred) scores are plotted for each sample. For all samples, the sequence quality (Phred) score was > 30 for more than 85% of the base pairs, indicating that all samples passed the QC criteria.

    Article Snippet: The library was sequenced using the Illumina® MiSeq system, with the MiSeq Micro reagent kit v2 (2 × 150 bp reads; Illumina, Inc., USA).

    Techniques: Next-Generation Sequencing, Plasmid Preparation, Incubation, Purification, Concentration Assay, Sequencing

    Normalised variants displayed in IGV. IGV display of Illumina MiSeq reads from a clinical patient highlighting the variation in the representation of indels within BAM files. The same variant is represented differently in three sets of reads which need to be merged to a single locus with the standardized HGVS nomenclature of NM_000314.4:c.21_22dup. Additionally, the reads contributing to the three read sets must be combined to calculate the correct variant allele frequency

    Journal: BMC Bioinformatics

    Article Title: Canary: an atomic pipeline for clinical amplicon assays

    doi: 10.1186/s12859-017-1950-z

    Figure Lengend Snippet: Normalised variants displayed in IGV. IGV display of Illumina MiSeq reads from a clinical patient highlighting the variation in the representation of indels within BAM files. The same variant is represented differently in three sets of reads which need to be merged to a single locus with the standardized HGVS nomenclature of NM_000314.4:c.21_22dup. Additionally, the reads contributing to the three read sets must be combined to calculate the correct variant allele frequency

    Article Snippet: The samples were run on an Illumina MiSeq sequencer and the reads converted to paired-end FASTQ files.

    Techniques: Variant Assay

    (a): Phylogram indicating abundance (number of sequences) of 16S rRNA sequences recovered from Stn1 (in red colour) and Stn3 (in blue colour) by Illumina MiSeq sequencing. Taxa showing differential distribution between the two stations have been shown. (b): Phylogram indicating the abundance (number of sequences) of 16S rRNA sequences recovered from Stn1 (in red colour) and Stn3 (in blue colour) from the clone libraries followed by Sanger sequencing. Taxa showing differential distribution between the two stations have been shown.

    Journal: Genomics Data

    Article Title: Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis

    doi: 10.1016/j.gdata.2016.11.017

    Figure Lengend Snippet: (a): Phylogram indicating abundance (number of sequences) of 16S rRNA sequences recovered from Stn1 (in red colour) and Stn3 (in blue colour) by Illumina MiSeq sequencing. Taxa showing differential distribution between the two stations have been shown. (b): Phylogram indicating the abundance (number of sequences) of 16S rRNA sequences recovered from Stn1 (in red colour) and Stn3 (in blue colour) from the clone libraries followed by Sanger sequencing. Taxa showing differential distribution between the two stations have been shown.

    Article Snippet: High-throughput sequencing using Illumina MiSeq approach was then undertaken from the same set of environmental DNA to obtain a deeper insight into bacterioplankton community structure.

    Techniques: Sequencing