Journal: Journal of Experimental Botany
Article Title: WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress
Figure Lengend Snippet: RNA-seq analysis of wild-type (WT) and wsl5 seedlings grown in C25 conditions. mRNA was enriched using oligo(dT) primers from total RNA isolated from 10-day-old (third leaf) seedlings of the WT and wsl5 using oligo(dT). cDNA was synthesized using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. (A) Frequencies of detected genes sorted according to expression levels. (B) Read numbers of WT and wsl5 sequences. (C) Volcano plot showing the overall alterations in gene expression in the wild type and wsl5 . (D) qRT-PCR analysis of genes differentially expressed in RNA-seq. Five up-regulated and five down-regulated genes were tested. Error bars represent the SD from three independent experiments (Student’s t -test, * P
Article Snippet: The library was constructed and sequenced using an Illumina Hisequation 2000 (TGS, Shenzhen).
Techniques: RNA Sequencing Assay, Isolation, Synthesized, Random Hexamer Labeling, Construct, Expressing, Quantitative RT-PCR