illumina hiseq 2000 Search Results


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  • 99
    Illumina Inc illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 44760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Illumina Inc
    Average 99 stars, based on 44760 article reviews
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    93
    Agilent technologies illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Agilent technologies
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    93
    PerkinElmer illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    Fasteris Life illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Fasteris Life, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Novogene illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Novogene, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    Beijing Genomics Institute Shenzhen illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Beijing Genomics Institute Shenzhen
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    Macrogen illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Macrogen, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    LGC Genomics GmbH illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/LGC Genomics GmbH
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    92
    BGI Shenzhen illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 92/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/BGI Shenzhen
    Average 92 stars, based on 87 article reviews
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    LC Sciences illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by LC Sciences, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/LC Sciences
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    91
    Integragen illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Integragen, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Integragen
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    GATC Biotech illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/GATC Biotech
    Average 92 stars, based on 25 article reviews
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    Otogenetics Corporation illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Otogenetics Corporation, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Otogenetics Corporation
    Average 92 stars, based on 2 article reviews
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    Eurofins illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Eurofins, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Eurofins
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    93
    Solexa illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Solexa, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Solexa
    Average 93 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    93
    Vazyme Biotech Co illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Vazyme Biotech Co
    Average 93 stars, based on 24 article reviews
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    93
    Biomarker Technologies Co illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Biomarker Technologies Co, used in various techniques. Bioz Stars score: 93/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Biomarker Technologies Co
    Average 93 stars, based on 189 article reviews
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    93
    Beckman Coulter illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Beckman Coulter
    Average 93 stars, based on 8 article reviews
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    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    91
    Oxford Gene Technology illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Oxford Gene Technology, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Oxford Gene Technology
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2000 - by Bioz Stars, 2020-07
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    92
    Illumina Inc illumins hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumins Hiseq 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumins hiseq 2000/product/Illumina Inc
    Average 92 stars, based on 15 article reviews
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    illumins hiseq 2000 - by Bioz Stars, 2020-07
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    91
    Solexa hiseq 2000 illumina solexa
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Hiseq 2000 Illumina Solexa, supplied by Solexa, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 illumina solexa/product/Solexa
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    91
    Illumina Inc illumina hisequation 2000
    Differential expression of plastid-encoded genes in the wild type (WT) and wsl5 grown in C25 conditions. mRNA was enriched from total RNA using Ribo-Zero rRNA Removal Kits for chloroplast-encoded genes from 10-day-old seedlings of the WT and wsl5. mRNA was fragmented and reverse-transcribed using random hexamer primers. The library was then constructed and sequenced using an <t>Illumina</t> HiSEquation 2000. The graph shows the log 2 ratio of transcript levels in the wsl5 mutant compared with the WT.
    Illumina Hisequation 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 715 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hisequation 2000/product/Illumina Inc
    Average 91 stars, based on 715 article reviews
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    illumina hisequation 2000 - by Bioz Stars, 2020-07
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    Image Search Results


    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using Illumina Hiseq 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.

    Journal: PLoS ONE

    Article Title: Systematic Analysis of Small RNAs Associated with Human Mitochondria by Deep Sequencing: Detailed Analysis of Mitochondrial Associated miRNA

    doi: 10.1371/journal.pone.0044873

    Figure Lengend Snippet: Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using Illumina Hiseq 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.

    Article Snippet: Bioinformatics Analysis of sRNA Libraries from Mitochondria All the 50 nt sequence tags generated from Illumina HiSeq 2000 went through data cleaning process which included getting rid of low quality tags.

    Techniques: Generated, Sequencing

    Differential expression of plastid-encoded genes in the wild type (WT) and wsl5 grown in C25 conditions. mRNA was enriched from total RNA using Ribo-Zero rRNA Removal Kits for chloroplast-encoded genes from 10-day-old seedlings of the WT and wsl5. mRNA was fragmented and reverse-transcribed using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. The graph shows the log 2 ratio of transcript levels in the wsl5 mutant compared with the WT.

    Journal: Journal of Experimental Botany

    Article Title: WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress

    doi: 10.1093/jxb/ery214

    Figure Lengend Snippet: Differential expression of plastid-encoded genes in the wild type (WT) and wsl5 grown in C25 conditions. mRNA was enriched from total RNA using Ribo-Zero rRNA Removal Kits for chloroplast-encoded genes from 10-day-old seedlings of the WT and wsl5. mRNA was fragmented and reverse-transcribed using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. The graph shows the log 2 ratio of transcript levels in the wsl5 mutant compared with the WT.

    Article Snippet: The library was constructed and sequenced using an Illumina Hisequation 2000 (TGS, Shenzhen).

    Techniques: Expressing, Random Hexamer Labeling, Construct, Mutagenesis

    RNA-seq analysis of wild-type (WT) and wsl5 seedlings grown in C25 conditions. mRNA was enriched using oligo(dT) primers from total RNA isolated from 10-day-old (third leaf) seedlings of the WT and wsl5 using oligo(dT). cDNA was synthesized using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. (A) Frequencies of detected genes sorted according to expression levels. (B) Read numbers of WT and wsl5 sequences. (C) Volcano plot showing the overall alterations in gene expression in the wild type and wsl5 . (D) qRT-PCR analysis of genes differentially expressed in RNA-seq. Five up-regulated and five down-regulated genes were tested. Error bars represent the SD from three independent experiments (Student’s t -test, * P

    Journal: Journal of Experimental Botany

    Article Title: WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress

    doi: 10.1093/jxb/ery214

    Figure Lengend Snippet: RNA-seq analysis of wild-type (WT) and wsl5 seedlings grown in C25 conditions. mRNA was enriched using oligo(dT) primers from total RNA isolated from 10-day-old (third leaf) seedlings of the WT and wsl5 using oligo(dT). cDNA was synthesized using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. (A) Frequencies of detected genes sorted according to expression levels. (B) Read numbers of WT and wsl5 sequences. (C) Volcano plot showing the overall alterations in gene expression in the wild type and wsl5 . (D) qRT-PCR analysis of genes differentially expressed in RNA-seq. Five up-regulated and five down-regulated genes were tested. Error bars represent the SD from three independent experiments (Student’s t -test, * P

    Article Snippet: The library was constructed and sequenced using an Illumina Hisequation 2000 (TGS, Shenzhen).

    Techniques: RNA Sequencing Assay, Isolation, Synthesized, Random Hexamer Labeling, Construct, Expressing, Quantitative RT-PCR