il-6 Biolegend Search Results


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  • 85
    BioLegend il 6 biolegend antibody
    RNF13 regulates muscle regeneration by enhancing <t>IL-4/IL-6</t> expression . (A) Neutralizing antibodies against IL-4 and IL-6 (or control IgG antibodies) were injected into the TA muscles of RNF13 +/+ and RNF13 -/- mice before CTX damage. Sections from TA muscles damaged for 5 d were stained with H E. Scale bar = 200 μm. (B and C) The CSAs of regenerating muscle fibers were analyzed in H E-stained sections. Five pairs of mice were used at each time point, and more than 10 sections from each mouse were analyzed. (D) Frozen sections of TA muscles, injected with anti-IL-4/IL-6 or control antibodies, were immunostained for Pax7 (red), MyoD (green), and nuclei (DAPI; blue). The bottom panel shows a merged image. (E and F) Pax7 + and Pax7 + /MyoD + cells in defined areas were counted. More than 50 sections from five individuals were analyzed. (G) Frozen sections of TA muscles injected with anti-IL-4/IL-6 or control antibodies were immunostained for BrdU (red) and MyoD (green). (H) The percentage of BrdU + cells in the MyoD + cell population was determined. More than 50 sections from five individuals were analyzed. (I) Pax7 and MyoD expression in the damaged muscle injected with anti-IL-4/IL-6 or control antibodies was detected by Western blot. Data are presented as means ± SEs (error bars; * P
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    BioLegend il 6 biolegend levels
    B cell deficient (BCD) mice displayed markedly less liver damage, anti-PLT Ig, thrombocytopenia and TNF expression versus wild type mice. TAA-mediated induction of circulating AST ( A ), ALT ( B ), anti-PLT Ig ( C ; WT Day 0 groups were normalized to 1 fold), PLT counts ( D ), TNF-α ( E ), HMGB1 ( F ), and <t>IL-6</t> ( G ) levels in B cell deficient (BCD) vs. wild type (WT) mice were shown. n = 6, # P
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    BioLegend il 6 biolegend elisas
    B cell deficient (BCD) mice displayed markedly less liver damage, anti-PLT Ig, thrombocytopenia and TNF expression versus wild type mice. TAA-mediated induction of circulating AST ( A ), ALT ( B ), anti-PLT Ig ( C ; WT Day 0 groups were normalized to 1 fold), PLT counts ( D ), TNF-α ( E ), HMGB1 ( F ), and <t>IL-6</t> ( G ) levels in B cell deficient (BCD) vs. wild type (WT) mice were shown. n = 6, # P
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    BioLegend biolegend anti il 6
    B cell deficient (BCD) mice displayed markedly less liver damage, anti-PLT Ig, thrombocytopenia and TNF expression versus wild type mice. TAA-mediated induction of circulating AST ( A ), ALT ( B ), anti-PLT Ig ( C ; WT Day 0 groups were normalized to 1 fold), PLT counts ( D ), TNF-α ( E ), HMGB1 ( F ), and <t>IL-6</t> ( G ) levels in B cell deficient (BCD) vs. wild type (WT) mice were shown. n = 6, # P
    Biolegend Anti Il 6, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend murine il 6
    TLR2 and Mal are required for induction of <t>IL-6</t> in LCMV-Arm-infected cells. Immortalized bone marrow-derived macrophages (iBMDM) from C57BL6/J mice (wild type) and from TLR2 (NR-9457), CD14 (NR-9570), and Mal (NR-9459) knockout mice were purchased from
    Murine Il 6, supplied by BioLegend, used in various techniques. Bioz Stars score: 91/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend il 6 apc
    TLR2 and Mal are required for induction of <t>IL-6</t> in LCMV-Arm-infected cells. Immortalized bone marrow-derived macrophages (iBMDM) from C57BL6/J mice (wild type) and from TLR2 (NR-9457), CD14 (NR-9570), and Mal (NR-9459) knockout mice were purchased from
    Il 6 Apc, supplied by BioLegend, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend interleukin il 6
    High frequency of IL-6Rα expression on CD4+ ILC1. Fresh or frozen PBMC were analyzed for ( A ) IL-6Rα and gp130 expression on ILC subsets and ( B ) phosphorylation of STAT-1 and STAT-3 following a 20-minute stimulation with 5 ng/mL of <t>IL-6.</t>
    Interleukin Il 6, supplied by BioLegend, used in various techniques. Bioz Stars score: 90/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend il 6 kits
    Host renal cell production of cytokines and cytotoxicity in response to ESBL isolate 7. A) <t>IL-6</t> and IL-8 production from A498 renal epithelial cells after stimulation for 6 h with the original ESBL7 isolate or with ESBL7 that have been pre-exposed 20 times to CORM-2 (250 μM) or vehicle (2.5% DMSO). B) Host renal cell cytotoxicity measured as LDH-release during the same conditions as in panel A and normalized to unstimulated and lysed control cells. Data are presented as mean ± SEM from three independent experiments. Asterisk denotes statistical significance (*p
    Il 6 Kits, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend sandwich biolegend legend max mouse il 6 elisa kit
    Host renal cell production of cytokines and cytotoxicity in response to ESBL isolate 7. A) <t>IL-6</t> and IL-8 production from A498 renal epithelial cells after stimulation for 6 h with the original ESBL7 isolate or with ESBL7 that have been pre-exposed 20 times to CORM-2 (250 μM) or vehicle (2.5% DMSO). B) Host renal cell cytotoxicity measured as LDH-release during the same conditions as in panel A and normalized to unstimulated and lysed control cells. Data are presented as mean ± SEM from three independent experiments. Asterisk denotes statistical significance (*p
    Sandwich Biolegend Legend Max Mouse Il 6 Elisa Kit, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend murine recombinant il 6
    <t>IL-6</t> infusion elicits a platelet activation response similar to DSS-colitis
    Murine Recombinant Il 6, supplied by BioLegend, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend il 6 elisa kits
    Effect of daphnetin on cytokines secretion in cell culture supernatant. The levels of cytokines IL-2 (A), IFN-γ (B), IL-4 (C) and <t>IL-6</t> (D) were analyzed after culturing the splenocytes in the presence of different concentration daphnetin and ConA (5 µg/mL) for 24 h. The cytokine levels were quantified by <t>ELISA.</t> The experiments were performed in triplicates and the data were present as means ± SD. ## P
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    BioLegend fitc il 6
    Effect of daphnetin on cytokines secretion in cell culture supernatant. The levels of cytokines IL-2 (A), IFN-γ (B), IL-4 (C) and <t>IL-6</t> (D) were analyzed after culturing the splenocytes in the presence of different concentration daphnetin and ConA (5 µg/mL) for 24 h. The cytokine levels were quantified by <t>ELISA.</t> The experiments were performed in triplicates and the data were present as means ± SD. ## P
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    BioLegend pe anti mouse il 6
    <t>IL-6</t> and IL-13 secretion by mast cells based on treatment. Raw cytokine production for IL-6 (a) and IL-13 (c) normalized as secretion per 10 6 cells. (b) IL-6 production in resting (no IgE-crosslink) BMMCs only (emphasis from 2a above). * in (a) and (b) indicates significant difference from no IgE-crosslink, no TGF-β1, no SCF control. * in (c) indicates significant difference from IgE-crosslinked, no TGF-β1, no SCF control; † indicates significant difference from IgE-crosslinked, no TGF-β1, SCF-treated control. Significance was noted at p
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    BioLegend il 6 elisa
    <t>IL-6</t> and IL-13 secretion by mast cells based on treatment. Raw cytokine production for IL-6 (a) and IL-13 (c) normalized as secretion per 10 6 cells. (b) IL-6 production in resting (no IgE-crosslink) BMMCs only (emphasis from 2a above). * in (a) and (b) indicates significant difference from no IgE-crosslink, no TGF-β1, no SCF control. * in (c) indicates significant difference from IgE-crosslinked, no TGF-β1, no SCF control; † indicates significant difference from IgE-crosslinked, no TGF-β1, SCF-treated control. Significance was noted at p
    Il 6 Elisa, supplied by BioLegend, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend pe conjugated il 6
    <t>IL-6</t> and IL-13 secretion by mast cells based on treatment. Raw cytokine production for IL-6 (a) and IL-13 (c) normalized as secretion per 10 6 cells. (b) IL-6 production in resting (no IgE-crosslink) BMMCs only (emphasis from 2a above). * in (a) and (b) indicates significant difference from no IgE-crosslink, no TGF-β1, no SCF control. * in (c) indicates significant difference from IgE-crosslinked, no TGF-β1, no SCF control; † indicates significant difference from IgE-crosslinked, no TGF-β1, SCF-treated control. Significance was noted at p
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    Image Search Results


    RNF13 regulates muscle regeneration by enhancing IL-4/IL-6 expression . (A) Neutralizing antibodies against IL-4 and IL-6 (or control IgG antibodies) were injected into the TA muscles of RNF13 +/+ and RNF13 -/- mice before CTX damage. Sections from TA muscles damaged for 5 d were stained with H E. Scale bar = 200 μm. (B and C) The CSAs of regenerating muscle fibers were analyzed in H E-stained sections. Five pairs of mice were used at each time point, and more than 10 sections from each mouse were analyzed. (D) Frozen sections of TA muscles, injected with anti-IL-4/IL-6 or control antibodies, were immunostained for Pax7 (red), MyoD (green), and nuclei (DAPI; blue). The bottom panel shows a merged image. (E and F) Pax7 + and Pax7 + /MyoD + cells in defined areas were counted. More than 50 sections from five individuals were analyzed. (G) Frozen sections of TA muscles injected with anti-IL-4/IL-6 or control antibodies were immunostained for BrdU (red) and MyoD (green). (H) The percentage of BrdU + cells in the MyoD + cell population was determined. More than 50 sections from five individuals were analyzed. (I) Pax7 and MyoD expression in the damaged muscle injected with anti-IL-4/IL-6 or control antibodies was detected by Western blot. Data are presented as means ± SEs (error bars; * P

    Journal: Protein & Cell

    Article Title: Accelerated regeneration of the skeletal muscle in RNF13-knockout mice is mediated by macrophage-secreted IL-4/IL-6

    doi: 10.1007/s13238-014-0025-4

    Figure Lengend Snippet: RNF13 regulates muscle regeneration by enhancing IL-4/IL-6 expression . (A) Neutralizing antibodies against IL-4 and IL-6 (or control IgG antibodies) were injected into the TA muscles of RNF13 +/+ and RNF13 -/- mice before CTX damage. Sections from TA muscles damaged for 5 d were stained with H E. Scale bar = 200 μm. (B and C) The CSAs of regenerating muscle fibers were analyzed in H E-stained sections. Five pairs of mice were used at each time point, and more than 10 sections from each mouse were analyzed. (D) Frozen sections of TA muscles, injected with anti-IL-4/IL-6 or control antibodies, were immunostained for Pax7 (red), MyoD (green), and nuclei (DAPI; blue). The bottom panel shows a merged image. (E and F) Pax7 + and Pax7 + /MyoD + cells in defined areas were counted. More than 50 sections from five individuals were analyzed. (G) Frozen sections of TA muscles injected with anti-IL-4/IL-6 or control antibodies were immunostained for BrdU (red) and MyoD (green). (H) The percentage of BrdU + cells in the MyoD + cell population was determined. More than 50 sections from five individuals were analyzed. (I) Pax7 and MyoD expression in the damaged muscle injected with anti-IL-4/IL-6 or control antibodies was detected by Western blot. Data are presented as means ± SEs (error bars; * P

    Article Snippet: For rescue experiments, IL-4 and IL-6 antibodies (10 μL of 1 μg/μL in PBS; Biolegend) were injected into the TA muscle 2 h before CTX injection.

    Techniques: Expressing, Injection, Mouse Assay, Staining, Western Blot

    AF-driven expansion of CD14 + HLA-DR -/low MDSC was dependent on IL-6/IL10-STAT3 signal pathway PBMC from HD (n = 3) were treated with the AF (50% v/v) from OC patients (n=4) in the presence of neutralizing antibodies against IL-6 and/or IL-10 (A) or STAT3 inhibitor stattic (B) for 48 hours and then analyzed for the abundance of CD14 + HLA-DR -/low MDSC by flow cytometry. Addition of isotype antibody or DMSO was as controls. The representative dotplots were shown in left panel and the statistics were shown in right graph. The data are expressed as mean ± SEM of 4 biological replicates and representative of three independent experiments. *p

    Journal: Oncotarget

    Article Title: Ascites-derived IL-6 and IL-10 synergistically expand CD14+HLA-DR-/low myeloid-derived suppressor cells in ovarian cancer patients

    doi: 10.18632/oncotarget.20164

    Figure Lengend Snippet: AF-driven expansion of CD14 + HLA-DR -/low MDSC was dependent on IL-6/IL10-STAT3 signal pathway PBMC from HD (n = 3) were treated with the AF (50% v/v) from OC patients (n=4) in the presence of neutralizing antibodies against IL-6 and/or IL-10 (A) or STAT3 inhibitor stattic (B) for 48 hours and then analyzed for the abundance of CD14 + HLA-DR -/low MDSC by flow cytometry. Addition of isotype antibody or DMSO was as controls. The representative dotplots were shown in left panel and the statistics were shown in right graph. The data are expressed as mean ± SEM of 4 biological replicates and representative of three independent experiments. *p

    Article Snippet: In some instances, PBMC were cultured with the AF in the presence of neutralizing antibodies against IL-6 and/or IL-10 (Biolegend) or STAT3 inhibitor stattic (Selleck) with isotype antibody or DMSO as controls.

    Techniques: Flow Cytometry, Cytometry

    The correlation between IL-6 and IL-10 levels and the abundance of CD14 + HLA-DR -/low MDSC in the ascites (A) IL-6 concentration in the sera and/or accompanying ascites from HD (n = 21) or OC (n = 11) patients. (B) IL-10 concentration in the sera and/or accompanying ascites from HD or OC patients. (C) The correlation between the abundance of CD14 + HLA-DR –/lo MDSC and IL-6 in ascites from OC patients (n = 11). (D) The correlation between the abundance of CD14 + HLA-DR –/lo MDSC and IL-10 in ascites from OC patients (n = 11). ***p

    Journal: Oncotarget

    Article Title: Ascites-derived IL-6 and IL-10 synergistically expand CD14+HLA-DR-/low myeloid-derived suppressor cells in ovarian cancer patients

    doi: 10.18632/oncotarget.20164

    Figure Lengend Snippet: The correlation between IL-6 and IL-10 levels and the abundance of CD14 + HLA-DR -/low MDSC in the ascites (A) IL-6 concentration in the sera and/or accompanying ascites from HD (n = 21) or OC (n = 11) patients. (B) IL-10 concentration in the sera and/or accompanying ascites from HD or OC patients. (C) The correlation between the abundance of CD14 + HLA-DR –/lo MDSC and IL-6 in ascites from OC patients (n = 11). (D) The correlation between the abundance of CD14 + HLA-DR –/lo MDSC and IL-10 in ascites from OC patients (n = 11). ***p

    Article Snippet: In some instances, PBMC were cultured with the AF in the presence of neutralizing antibodies against IL-6 and/or IL-10 (Biolegend) or STAT3 inhibitor stattic (Selleck) with isotype antibody or DMSO as controls.

    Techniques: Concentration Assay

    The correlation between relapse-free survival (RFS) and the abundance of CD14 + HLA-DR -/low MDSC and the levels of IL-6 and IL-10 in the OC patients (A) Kaplan-Meier plots showing the correlation between RFS and high or low levels (median as cutoff) of CD14 + HLA-DR –/lo MDSC in the PB (A) or accompanying ascites (B) , and IL-6 (C) or IL-10 (D) concentration in the accompanying ascites in the OC patients (n = 21 for PB and n = 11 for ascites) with p-Values determined by Mantel–Cox log-rank test.

    Journal: Oncotarget

    Article Title: Ascites-derived IL-6 and IL-10 synergistically expand CD14+HLA-DR-/low myeloid-derived suppressor cells in ovarian cancer patients

    doi: 10.18632/oncotarget.20164

    Figure Lengend Snippet: The correlation between relapse-free survival (RFS) and the abundance of CD14 + HLA-DR -/low MDSC and the levels of IL-6 and IL-10 in the OC patients (A) Kaplan-Meier plots showing the correlation between RFS and high or low levels (median as cutoff) of CD14 + HLA-DR –/lo MDSC in the PB (A) or accompanying ascites (B) , and IL-6 (C) or IL-10 (D) concentration in the accompanying ascites in the OC patients (n = 21 for PB and n = 11 for ascites) with p-Values determined by Mantel–Cox log-rank test.

    Article Snippet: In some instances, PBMC were cultured with the AF in the presence of neutralizing antibodies against IL-6 and/or IL-10 (Biolegend) or STAT3 inhibitor stattic (Selleck) with isotype antibody or DMSO as controls.

    Techniques: Concentration Assay

    B cell deficient (BCD) mice displayed markedly less liver damage, anti-PLT Ig, thrombocytopenia and TNF expression versus wild type mice. TAA-mediated induction of circulating AST ( A ), ALT ( B ), anti-PLT Ig ( C ; WT Day 0 groups were normalized to 1 fold), PLT counts ( D ), TNF-α ( E ), HMGB1 ( F ), and IL-6 ( G ) levels in B cell deficient (BCD) vs. wild type (WT) mice were shown. n = 6, # P

    Journal: Scientific Reports

    Article Title: Thioacetamide-induced liver damage and thrombocytopenia is associated with induction of antiplatelet autoantibody in mice

    doi: 10.1038/s41598-019-53977-7

    Figure Lengend Snippet: B cell deficient (BCD) mice displayed markedly less liver damage, anti-PLT Ig, thrombocytopenia and TNF expression versus wild type mice. TAA-mediated induction of circulating AST ( A ), ALT ( B ), anti-PLT Ig ( C ; WT Day 0 groups were normalized to 1 fold), PLT counts ( D ), TNF-α ( E ), HMGB1 ( F ), and IL-6 ( G ) levels in B cell deficient (BCD) vs. wild type (WT) mice were shown. n = 6, # P

    Article Snippet: The ELISA kit used for analysing plasma levels of proinflammatory cytokine tumour necrosis factor-α (TNF-α), high mobility group protein B1 (HMGB-1), and interleukin-6 (IL-6) levels was purchased from BioLegend (San Diego, CA, USA).

    Techniques: Mouse Assay, Expressing, AST Assay

    TLR2 and Mal are required for induction of IL-6 in LCMV-Arm-infected cells. Immortalized bone marrow-derived macrophages (iBMDM) from C57BL6/J mice (wild type) and from TLR2 (NR-9457), CD14 (NR-9570), and Mal (NR-9459) knockout mice were purchased from

    Journal: Journal of Virology

    Article Title: Pathogenic Old World Arenaviruses Inhibit TLR2/Mal-Dependent Proinflammatory Cytokines In Vitro

    doi: 10.1128/JVI.06508-11

    Figure Lengend Snippet: TLR2 and Mal are required for induction of IL-6 in LCMV-Arm-infected cells. Immortalized bone marrow-derived macrophages (iBMDM) from C57BL6/J mice (wild type) and from TLR2 (NR-9457), CD14 (NR-9570), and Mal (NR-9459) knockout mice were purchased from

    Article Snippet: The iBMDM derived from WT and KO mice were infected with LCMV strains (MOI = 1), and culture supernatant samples were collected at 24, 48, and 72 hpi and analyzed for the production of murine IL-6 (BioLegend).

    Techniques: Infection, Derivative Assay, Mouse Assay, Knock-Out

    Effects of MHY1485 on FcεRI-mediated degranulation and cytokine production in mast cells. BMMCs were sensitized for 5 h, incubated with the indicated concentration of MHY1485 for 1 h, and then either unstimulated (unstim) or stimulated with the DNP-HSA Ag. (A) Time-course immunoblot analysis for mTOR signaling, with or without MHY1485, following Ag stimulation. Band densities of pS6K on Thr389 and pAkt on Ser473 were normalized to their total protein expression from the results of 3 independent experiments. AU represents arbitrary unit. (B) Degranulation was assessed by measuring β-hexosaminidase release 30 min after stimulation. (C) The levels of IL-6 and TNF-α proteins in the media were analyzed using ELISA 6 h after stimulation. (D) qRT-PCR analysis for FcεRI-mediated induction of Il6 and Tfna mRNA was carried out 1 h after Ag stimulation. Bar graphs are shown as mean±standard error of the mean of triplicates and are representative of 3 independent experiments. A p-value of less than 0.05 between stimulated groups with and without MHY1485 treatment is judged significant and indicated. pS6K, phospho-S6K; Thr389, threonine 389; pAkt, phospho-Akt; Ser473, serine 473; qRT-PCR, quantitative real-time PCR. * p

    Journal: Immune Network

    Article Title: Inhibition of Mast Cell Function and Proliferation by mTOR Activator MHY1485

    doi: 10.4110/in.2018.18.e18

    Figure Lengend Snippet: Effects of MHY1485 on FcεRI-mediated degranulation and cytokine production in mast cells. BMMCs were sensitized for 5 h, incubated with the indicated concentration of MHY1485 for 1 h, and then either unstimulated (unstim) or stimulated with the DNP-HSA Ag. (A) Time-course immunoblot analysis for mTOR signaling, with or without MHY1485, following Ag stimulation. Band densities of pS6K on Thr389 and pAkt on Ser473 were normalized to their total protein expression from the results of 3 independent experiments. AU represents arbitrary unit. (B) Degranulation was assessed by measuring β-hexosaminidase release 30 min after stimulation. (C) The levels of IL-6 and TNF-α proteins in the media were analyzed using ELISA 6 h after stimulation. (D) qRT-PCR analysis for FcεRI-mediated induction of Il6 and Tfna mRNA was carried out 1 h after Ag stimulation. Bar graphs are shown as mean±standard error of the mean of triplicates and are representative of 3 independent experiments. A p-value of less than 0.05 between stimulated groups with and without MHY1485 treatment is judged significant and indicated. pS6K, phospho-S6K; Thr389, threonine 389; pAkt, phospho-Akt; Ser473, serine 473; qRT-PCR, quantitative real-time PCR. * p

    Article Snippet: To measure secreted cytokines, supernatants were harvested after 6 h and tested for their levels of cytokines using ELISAs with Mouse IL-6 and TNF-α ELISA MAX Deluxe kits (BioLegend, San Diego, CA, USA) according to the manufacturer's instructions.

    Techniques: Incubation, Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    High frequency of IL-6Rα expression on CD4+ ILC1. Fresh or frozen PBMC were analyzed for ( A ) IL-6Rα and gp130 expression on ILC subsets and ( B ) phosphorylation of STAT-1 and STAT-3 following a 20-minute stimulation with 5 ng/mL of IL-6.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: CD4+ group 1 innate lymphoid cells form a functionally distinct ILC subset that is increased in systemic sclerosis

    doi: 10.4049/jimmunol.1501491

    Figure Lengend Snippet: High frequency of IL-6Rα expression on CD4+ ILC1. Fresh or frozen PBMC were analyzed for ( A ) IL-6Rα and gp130 expression on ILC subsets and ( B ) phosphorylation of STAT-1 and STAT-3 following a 20-minute stimulation with 5 ng/mL of IL-6.

    Article Snippet: Stimulations were performed with 5 ng/mL recombinant IL-6 (Biolegend) in cRPMI at 37°C/5% CO2 for 20 minutes after a 30 minute rest (10 × 106 cells/mL in cRPMI/1% HS; 37°C/5% CO2) and staining with viability dye and surface markers.

    Techniques: Expressing

    Host renal cell production of cytokines and cytotoxicity in response to ESBL isolate 7. A) IL-6 and IL-8 production from A498 renal epithelial cells after stimulation for 6 h with the original ESBL7 isolate or with ESBL7 that have been pre-exposed 20 times to CORM-2 (250 μM) or vehicle (2.5% DMSO). B) Host renal cell cytotoxicity measured as LDH-release during the same conditions as in panel A and normalized to unstimulated and lysed control cells. Data are presented as mean ± SEM from three independent experiments. Asterisk denotes statistical significance (*p

    Journal: PLoS ONE

    Article Title: Global gene expression profiling and antibiotic susceptibility after repeated exposure to the carbon monoxide-releasing molecule-2 (CORM-2) in multidrug-resistant ESBL-producing uropathogenic Escherichia coli

    doi: 10.1371/journal.pone.0178541

    Figure Lengend Snippet: Host renal cell production of cytokines and cytotoxicity in response to ESBL isolate 7. A) IL-6 and IL-8 production from A498 renal epithelial cells after stimulation for 6 h with the original ESBL7 isolate or with ESBL7 that have been pre-exposed 20 times to CORM-2 (250 μM) or vehicle (2.5% DMSO). B) Host renal cell cytotoxicity measured as LDH-release during the same conditions as in panel A and normalized to unstimulated and lysed control cells. Data are presented as mean ± SEM from three independent experiments. Asterisk denotes statistical significance (*p

    Article Snippet: IL-6 and IL-8 cytokine production were measured using human IL-8 and IL-6 kits (ELISA MAX™ Deluxe Sets, BioLegend, San Diego, CA, USA) according to manufacturer's protocol and measured on a spectrophotometer (Multiscan Ascent).

    Techniques:

    IL-6 infusion elicits a platelet activation response similar to DSS-colitis

    Journal: Inflammatory bowel diseases

    Article Title: The platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6

    doi: 10.1097/01.MIB.0000440614.83703.84

    Figure Lengend Snippet: IL-6 infusion elicits a platelet activation response similar to DSS-colitis

    Article Snippet: Murine recombinant IL-6 (BioLegend) was infused at a rate of 20 pg/g/min by an micro-osmotic Alzet pump (MODEL 1007D; 0.5 μl/hour) that was implanted subcutaneously for 7 days.

    Techniques: Activation Assay

    Thrombus development in cremaster muscle arterioles of IL-6 infused and DSS colitic mice

    Journal: Inflammatory bowel diseases

    Article Title: The platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6

    doi: 10.1097/01.MIB.0000440614.83703.84

    Figure Lengend Snippet: Thrombus development in cremaster muscle arterioles of IL-6 infused and DSS colitic mice

    Article Snippet: Murine recombinant IL-6 (BioLegend) was infused at a rate of 20 pg/g/min by an micro-osmotic Alzet pump (MODEL 1007D; 0.5 μl/hour) that was implanted subcutaneously for 7 days.

    Techniques: Mouse Assay

    IL-6 deficiency prevents platelet activation in DSS-treated mice

    Journal: Inflammatory bowel diseases

    Article Title: The platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6

    doi: 10.1097/01.MIB.0000440614.83703.84

    Figure Lengend Snippet: IL-6 deficiency prevents platelet activation in DSS-treated mice

    Article Snippet: Murine recombinant IL-6 (BioLegend) was infused at a rate of 20 pg/g/min by an micro-osmotic Alzet pump (MODEL 1007D; 0.5 μl/hour) that was implanted subcutaneously for 7 days.

    Techniques: Activation Assay, Mouse Assay

    Effects of IL-6 deficiency on DSS-induced formation of platelet-leukocyte aggregates

    Journal: Inflammatory bowel diseases

    Article Title: The platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6

    doi: 10.1097/01.MIB.0000440614.83703.84

    Figure Lengend Snippet: Effects of IL-6 deficiency on DSS-induced formation of platelet-leukocyte aggregates

    Article Snippet: Murine recombinant IL-6 (BioLegend) was infused at a rate of 20 pg/g/min by an micro-osmotic Alzet pump (MODEL 1007D; 0.5 μl/hour) that was implanted subcutaneously for 7 days.

    Techniques:

    Chronic IL-6 infusion induces thrombocytosis responses similar to DSS colitis

    Journal: Inflammatory bowel diseases

    Article Title: The platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6

    doi: 10.1097/01.MIB.0000440614.83703.84

    Figure Lengend Snippet: Chronic IL-6 infusion induces thrombocytosis responses similar to DSS colitis

    Article Snippet: Murine recombinant IL-6 (BioLegend) was infused at a rate of 20 pg/g/min by an micro-osmotic Alzet pump (MODEL 1007D; 0.5 μl/hour) that was implanted subcutaneously for 7 days.

    Techniques:

    A comparison of platelet-leukocyte aggregate formation in IL-6 infused and DSS-colitic mice

    Journal: Inflammatory bowel diseases

    Article Title: The platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6

    doi: 10.1097/01.MIB.0000440614.83703.84

    Figure Lengend Snippet: A comparison of platelet-leukocyte aggregate formation in IL-6 infused and DSS-colitic mice

    Article Snippet: Murine recombinant IL-6 (BioLegend) was infused at a rate of 20 pg/g/min by an micro-osmotic Alzet pump (MODEL 1007D; 0.5 μl/hour) that was implanted subcutaneously for 7 days.

    Techniques: Mouse Assay

    IL-6 deficiency prevents DSS-induced thrombocytosis

    Journal: Inflammatory bowel diseases

    Article Title: The platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6

    doi: 10.1097/01.MIB.0000440614.83703.84

    Figure Lengend Snippet: IL-6 deficiency prevents DSS-induced thrombocytosis

    Article Snippet: Murine recombinant IL-6 (BioLegend) was infused at a rate of 20 pg/g/min by an micro-osmotic Alzet pump (MODEL 1007D; 0.5 μl/hour) that was implanted subcutaneously for 7 days.

    Techniques:

    Effect of daphnetin on cytokines secretion in cell culture supernatant. The levels of cytokines IL-2 (A), IFN-γ (B), IL-4 (C) and IL-6 (D) were analyzed after culturing the splenocytes in the presence of different concentration daphnetin and ConA (5 µg/mL) for 24 h. The cytokine levels were quantified by ELISA. The experiments were performed in triplicates and the data were present as means ± SD. ## P

    Journal: PLoS ONE

    Article Title: Immunosuppressive Activity of Daphnetin, One of Coumarin Derivatives, Is Mediated through Suppression of NF-?B and NFAT Signaling Pathways in Mouse T Cells

    doi: 10.1371/journal.pone.0096502

    Figure Lengend Snippet: Effect of daphnetin on cytokines secretion in cell culture supernatant. The levels of cytokines IL-2 (A), IFN-γ (B), IL-4 (C) and IL-6 (D) were analyzed after culturing the splenocytes in the presence of different concentration daphnetin and ConA (5 µg/mL) for 24 h. The cytokine levels were quantified by ELISA. The experiments were performed in triplicates and the data were present as means ± SD. ## P

    Article Snippet: The mAbs against mouse CD3, CD4, CD8, IFN-γ, IL-2, IL-4 and IL-6 ELISA kits were purchased from Biolegend (California, USA).

    Techniques: Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    The involvement of MAPK in rCRT-induced TNF-α and IL-6 protein expression. ( A , B ) MAPK activation in macrophages stimulated with various concentrations of MrCRT or OrCRT for 30 min ( A ), or with 10 μg/mL MrCRT or OrCRT for different time periods ( B ). Cell lysates were analyzed by SDS-PAGE and immunoblotted for total MAPK and phophorylated MAPK . Semi-quantitative densitometric analysis of Western blots was shown in the right panels; ( C , D ) The involvement of MAPK in rCRT-induced TNF-α and IL-6 production. Macrophages were pretreated with 30 μM SB203580, SP600125 and U0126, and then stimulated with 10 μg/mL MrCRT or OrCRT for 24 h. TNF-α ( C ) and IL-6 ( D ) in supernatant were quantitated by ELISA. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Soluble Calreticulin Induces Tumor Necrosis Factor-? (TNF-α) and Interleukin (IL)-6 Production by Macrophages through Mitogen-Activated Protein Kinase (MAPK) and NFκB Signaling Pathways

    doi: 10.3390/ijms15022916

    Figure Lengend Snippet: The involvement of MAPK in rCRT-induced TNF-α and IL-6 protein expression. ( A , B ) MAPK activation in macrophages stimulated with various concentrations of MrCRT or OrCRT for 30 min ( A ), or with 10 μg/mL MrCRT or OrCRT for different time periods ( B ). Cell lysates were analyzed by SDS-PAGE and immunoblotted for total MAPK and phophorylated MAPK . Semi-quantitative densitometric analysis of Western blots was shown in the right panels; ( C , D ) The involvement of MAPK in rCRT-induced TNF-α and IL-6 production. Macrophages were pretreated with 30 μM SB203580, SP600125 and U0126, and then stimulated with 10 μg/mL MrCRT or OrCRT for 24 h. TNF-α ( C ) and IL-6 ( D ) in supernatant were quantitated by ELISA. * p

    Article Snippet: TNF-α and IL-6 ELISA kit were from Biolegend (San Diego, CA, USA).

    Techniques: Expressing, Activation Assay, SDS Page, Western Blot, Enzyme-linked Immunosorbent Assay

    IL-6 and IL-13 secretion by mast cells based on treatment. Raw cytokine production for IL-6 (a) and IL-13 (c) normalized as secretion per 10 6 cells. (b) IL-6 production in resting (no IgE-crosslink) BMMCs only (emphasis from 2a above). * in (a) and (b) indicates significant difference from no IgE-crosslink, no TGF-β1, no SCF control. * in (c) indicates significant difference from IgE-crosslinked, no TGF-β1, no SCF control; † indicates significant difference from IgE-crosslinked, no TGF-β1, SCF-treated control. Significance was noted at p

    Journal: PLoS ONE

    Article Title: Soluble transforming growth factor beta-1 enhances murine mast cell release of Interleukin 6 in IgE-independent and Interleukin 13 in IgE-dependent settings in vitro

    doi: 10.1371/journal.pone.0207704

    Figure Lengend Snippet: IL-6 and IL-13 secretion by mast cells based on treatment. Raw cytokine production for IL-6 (a) and IL-13 (c) normalized as secretion per 10 6 cells. (b) IL-6 production in resting (no IgE-crosslink) BMMCs only (emphasis from 2a above). * in (a) and (b) indicates significant difference from no IgE-crosslink, no TGF-β1, no SCF control. * in (c) indicates significant difference from IgE-crosslinked, no TGF-β1, no SCF control; † indicates significant difference from IgE-crosslinked, no TGF-β1, SCF-treated control. Significance was noted at p

    Article Snippet: Appropriate groups received PE-conjugated IL-6 antibody (BioLegend, #504504) per the manufacturer’s recommendation and left to incubate in the dark for 20–30 minutes.

    Techniques:

    IgE-stimulated LAMP-1 translocation and early phase IL-6 production. Representative cytograms of (a) surface LAMP-1 expression (10 minute activation) and (c) intracellular IL-6 (90 minute activation) analyzed via flow cytometry. (b) Fold change of mean fluorescence intensity compared to baseline (no TGF-β1, no IgE crosslink) for LAMP-1 expression; * indicates significant difference from baseline, † indicates significant difference compared to TGF-β1-treated, IgE-crosslinked groups. (d) Representative histogram of intracellular IL-6. (e) Mean fluorescence intensity for intracellular IL-6. Significance was noted at p

    Journal: PLoS ONE

    Article Title: Soluble transforming growth factor beta-1 enhances murine mast cell release of Interleukin 6 in IgE-independent and Interleukin 13 in IgE-dependent settings in vitro

    doi: 10.1371/journal.pone.0207704

    Figure Lengend Snippet: IgE-stimulated LAMP-1 translocation and early phase IL-6 production. Representative cytograms of (a) surface LAMP-1 expression (10 minute activation) and (c) intracellular IL-6 (90 minute activation) analyzed via flow cytometry. (b) Fold change of mean fluorescence intensity compared to baseline (no TGF-β1, no IgE crosslink) for LAMP-1 expression; * indicates significant difference from baseline, † indicates significant difference compared to TGF-β1-treated, IgE-crosslinked groups. (d) Representative histogram of intracellular IL-6. (e) Mean fluorescence intensity for intracellular IL-6. Significance was noted at p

    Article Snippet: Appropriate groups received PE-conjugated IL-6 antibody (BioLegend, #504504) per the manufacturer’s recommendation and left to incubate in the dark for 20–30 minutes.

    Techniques: Translocation Assay, Expressing, Activation Assay, Flow Cytometry, Cytometry, Fluorescence

    IL-33-stimulated early phase IL-6 production. (a) Representative cytograms of intracellular IL-6 (90 minute activation) analyzed via flow cytometry. (b) Representative histogram of intracellular IL-6. (c) Mean fluorescence intensity for intracellular IL-6; * indicates significant difference (p

    Journal: PLoS ONE

    Article Title: Soluble transforming growth factor beta-1 enhances murine mast cell release of Interleukin 6 in IgE-independent and Interleukin 13 in IgE-dependent settings in vitro

    doi: 10.1371/journal.pone.0207704

    Figure Lengend Snippet: IL-33-stimulated early phase IL-6 production. (a) Representative cytograms of intracellular IL-6 (90 minute activation) analyzed via flow cytometry. (b) Representative histogram of intracellular IL-6. (c) Mean fluorescence intensity for intracellular IL-6; * indicates significant difference (p

    Article Snippet: Appropriate groups received PE-conjugated IL-6 antibody (BioLegend, #504504) per the manufacturer’s recommendation and left to incubate in the dark for 20–30 minutes.

    Techniques: Activation Assay, Flow Cytometry, Cytometry, Fluorescence

    Immunofluorescent study for IL-6- and IL-17-producing cells in WT and CD69KO mice. A) Predominant IL-6- and IL-17-producing cells in the lung of the two genotypes instilled with PPE. Lung sections from WT and CD69KO mice at 1 dpi were reacted with the designated combination of antibodies. Asterisks indicate double-positive cells. Scale bar represents 50 µm. B) The PPE-increased and CD69-deficinecy-sensitive IL-6 + Iba1 + , IL-17 + CD4 + and IL-17 + TCRγδ + cells. Data are shown as mean±S.E.M. (n=8). * P

    Journal: Biochemistry and Biophysics Reports

    Article Title: Role of CD69 in the pathogenesis of elastase-induced pulmonary inflammation and emphysema

    doi: 10.1016/j.bbrep.2016.07.010

    Figure Lengend Snippet: Immunofluorescent study for IL-6- and IL-17-producing cells in WT and CD69KO mice. A) Predominant IL-6- and IL-17-producing cells in the lung of the two genotypes instilled with PPE. Lung sections from WT and CD69KO mice at 1 dpi were reacted with the designated combination of antibodies. Asterisks indicate double-positive cells. Scale bar represents 50 µm. B) The PPE-increased and CD69-deficinecy-sensitive IL-6 + Iba1 + , IL-17 + CD4 + and IL-17 + TCRγδ + cells. Data are shown as mean±S.E.M. (n=8). * P

    Article Snippet: 2.4 Immunofluorescent study Freshly cut lung sections (5 µm thickness) from mice at 1 dpi were pretreated with FcR blocking agent (Miltenyi Biotec, Gladbach, Germany) for 15 min, and then reacted with various antibodies as follow: anti-mouse IL-6 antibody (Biolegend), anti-mouse IL-17A (Biolegend), anti-Iba1 antibody (WAKO, Osaka, Japan), anti-CD4 antibody (BIOSS ANTIBODIES, Boston, Massachusetts) and anti-mouse T-cell receptor (TCR)γδ antibody (Biolegend).

    Techniques: Mouse Assay

    Changes in cytokine levels in BALF from WT and CD69KO mice. Concentrations of IFN-γ, IL-17A, IL-6 and IL-23 in BALF from the two genotypes at 1 dpi were determined by ELISA. Data are shown as mean±S.E.M. (n=6). * P

    Journal: Biochemistry and Biophysics Reports

    Article Title: Role of CD69 in the pathogenesis of elastase-induced pulmonary inflammation and emphysema

    doi: 10.1016/j.bbrep.2016.07.010

    Figure Lengend Snippet: Changes in cytokine levels in BALF from WT and CD69KO mice. Concentrations of IFN-γ, IL-17A, IL-6 and IL-23 in BALF from the two genotypes at 1 dpi were determined by ELISA. Data are shown as mean±S.E.M. (n=6). * P

    Article Snippet: 2.4 Immunofluorescent study Freshly cut lung sections (5 µm thickness) from mice at 1 dpi were pretreated with FcR blocking agent (Miltenyi Biotec, Gladbach, Germany) for 15 min, and then reacted with various antibodies as follow: anti-mouse IL-6 antibody (Biolegend), anti-mouse IL-17A (Biolegend), anti-Iba1 antibody (WAKO, Osaka, Japan), anti-CD4 antibody (BIOSS ANTIBODIES, Boston, Massachusetts) and anti-mouse T-cell receptor (TCR)γδ antibody (Biolegend).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Effects of IL-6- and IL-17-neutralizing antibodies on elastase-induced lung emphysematous changes in WT and CD69KO mice. A) Typical histopathological changes of the lungs. Scale bar represents 100 µm. B) MLI, an index of enlargement of alveolar airspaces. Data are shown as mean±S.E.M. (n=4–6). * P

    Journal: Biochemistry and Biophysics Reports

    Article Title: Role of CD69 in the pathogenesis of elastase-induced pulmonary inflammation and emphysema

    doi: 10.1016/j.bbrep.2016.07.010

    Figure Lengend Snippet: Effects of IL-6- and IL-17-neutralizing antibodies on elastase-induced lung emphysematous changes in WT and CD69KO mice. A) Typical histopathological changes of the lungs. Scale bar represents 100 µm. B) MLI, an index of enlargement of alveolar airspaces. Data are shown as mean±S.E.M. (n=4–6). * P

    Article Snippet: 2.4 Immunofluorescent study Freshly cut lung sections (5 µm thickness) from mice at 1 dpi were pretreated with FcR blocking agent (Miltenyi Biotec, Gladbach, Germany) for 15 min, and then reacted with various antibodies as follow: anti-mouse IL-6 antibody (Biolegend), anti-mouse IL-17A (Biolegend), anti-Iba1 antibody (WAKO, Osaka, Japan), anti-CD4 antibody (BIOSS ANTIBODIES, Boston, Massachusetts) and anti-mouse T-cell receptor (TCR)γδ antibody (Biolegend).

    Techniques: Mouse Assay