il-17a mrna Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore quantitative il 17a mrna rt pcr assay el4 cells
    <t>IL-17a</t> <t>mRNA</t> expression in <t>EL4</t> cells treated with ligand 3 , 25 , and 23 (10 μM, 24h) or DMSO. The level of IL-17a expression was normalized to that of the GAPDH expression. All data are expressed as the mean ± s.d. ( n = 3). The relative gene expression was calculated by the 2 –ΔΔCt (Livak) method using the DMSO control as calibrator.
    Quantitative Il 17a Mrna Rt Pcr Assay El4 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative il 17a mrna rt pcr assay el4 cells/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    quantitative il 17a mrna rt pcr assay el4 cells - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    89
    SABiosciences actin mrna
    <t>Bcl3</t> mediates the BZ-induced IL-8 and IL-17 expression, and cIAP1 and cIAP2 inhibition in Hut-78 cells. (A) Suppression of Bcl3 <t>mRNA</t> (top left panel) and protein (lower left panel) levels by Bcl3 siRNA decreases viability in BZ-treated Hut-78 cells, measured by Trypan Blue exclusion (right panel). (B) Real time RT-PCR analysis of cIAP1, cIAP2 and Bcl2 mRNA levels in Hut-78 cells transfected with control siRNA (full columns) or Bcl3 specific siRNA (empty columns) and treated 24 h with 0, 10 and 100 nM BZ. (C) Real time RT-PCR analysis of IL-8 and IL-17 mRNA levels in Hut-78 cells transfected with control siRNA (full columns) or Bcl3 specific siRNA (empty columns) and treated 24 h with 0, 10 and 100 nM BZ. The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant ( p
    Actin Mrna, supplied by SABiosciences, used in various techniques. Bioz Stars score: 89/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/actin mrna/product/SABiosciences
    Average 89 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    actin mrna - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    93
    4Gene mrna expression
    The <t>mRNA</t> expression of IL - <t>17A</t> with the mRNA expression of IL - 4 , cytokine IL-4 and SCS values among different genotypes in Sanhe Cattle. a Relative mRNA expression of 3 genotypes of SNP in IL - 17A gene. b Relative mRNA expression of 3 genotypes of SNP in IL - 17A in association with IL-4 gene. c Comparison of the genotypes with respect to SCS. d Comparison of the 3 genotypes with respect to the IL-4 cytokines
    Mrna Expression, supplied by 4Gene, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrna expression/product/4Gene
    Average 93 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    mrna expression - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    IL-17a mRNA expression in EL4 cells treated with ligand 3 , 25 , and 23 (10 μM, 24h) or DMSO. The level of IL-17a expression was normalized to that of the GAPDH expression. All data are expressed as the mean ± s.d. ( n = 3). The relative gene expression was calculated by the 2 –ΔΔCt (Livak) method using the DMSO control as calibrator.

    Journal: Journal of Medicinal Chemistry

    Article Title: Ligand-Based Design of Allosteric Retinoic Acid Receptor-Related Orphan Receptor γt (RORγt) Inverse Agonists

    doi: 10.1021/acs.jmedchem.9b01372

    Figure Lengend Snippet: IL-17a mRNA expression in EL4 cells treated with ligand 3 , 25 , and 23 (10 μM, 24h) or DMSO. The level of IL-17a expression was normalized to that of the GAPDH expression. All data are expressed as the mean ± s.d. ( n = 3). The relative gene expression was calculated by the 2 –ΔΔCt (Livak) method using the DMSO control as calibrator.

    Article Snippet: 4.6 Quantitative IL-17a mRNA RT-PCR Assay EL4 cells (Sigma-Aldrich) were grown in DMEM (Gibco) with 10% FBS.

    Techniques: Expressing

    Bcl3 mediates the BZ-induced IL-8 and IL-17 expression, and cIAP1 and cIAP2 inhibition in Hut-78 cells. (A) Suppression of Bcl3 mRNA (top left panel) and protein (lower left panel) levels by Bcl3 siRNA decreases viability in BZ-treated Hut-78 cells, measured by Trypan Blue exclusion (right panel). (B) Real time RT-PCR analysis of cIAP1, cIAP2 and Bcl2 mRNA levels in Hut-78 cells transfected with control siRNA (full columns) or Bcl3 specific siRNA (empty columns) and treated 24 h with 0, 10 and 100 nM BZ. (C) Real time RT-PCR analysis of IL-8 and IL-17 mRNA levels in Hut-78 cells transfected with control siRNA (full columns) or Bcl3 specific siRNA (empty columns) and treated 24 h with 0, 10 and 100 nM BZ. The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant ( p

    Journal: Biochimica et biophysica acta

    Article Title: Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphoma

    doi: 10.1016/j.bbamcr.2014.07.012

    Figure Lengend Snippet: Bcl3 mediates the BZ-induced IL-8 and IL-17 expression, and cIAP1 and cIAP2 inhibition in Hut-78 cells. (A) Suppression of Bcl3 mRNA (top left panel) and protein (lower left panel) levels by Bcl3 siRNA decreases viability in BZ-treated Hut-78 cells, measured by Trypan Blue exclusion (right panel). (B) Real time RT-PCR analysis of cIAP1, cIAP2 and Bcl2 mRNA levels in Hut-78 cells transfected with control siRNA (full columns) or Bcl3 specific siRNA (empty columns) and treated 24 h with 0, 10 and 100 nM BZ. (C) Real time RT-PCR analysis of IL-8 and IL-17 mRNA levels in Hut-78 cells transfected with control siRNA (full columns) or Bcl3 specific siRNA (empty columns) and treated 24 h with 0, 10 and 100 nM BZ. The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant ( p

    Article Snippet: The primers used for the quantification of Bcl3, cIAP1, cIAP2, Bcl2, IL-8, IL-17, and actin mRNA were purchased from SA Biosciences (Frederick, MD, USA).

    Techniques: Expressing, Inhibition, Quantitative RT-PCR, Transfection

    Bcl3 is highly expressed in CTCL cells, and its expression is inhibited by BZ. Western blotting of whole cell extracts prepared from CTCL Hut-78 (A) and HH cells (B) treated with increasing concentrations of BZ for 24 h, and analyzed by using Bcl3 antibody. To confirm equal protein loading, the membranes were stripped and re-probed with actin antibody. Each lane corresponds to approximately 5 × 10 4 cells. (C) Real time RT-PCR analysis of Bcl3 mRNA levels in Hut-78 cells treated 24 h with increasing BZ concentrations. (D) Real time RT-PCR analysis of Bcl3 mRNA levels in Hut-78 cells treated 0, 6, 24 and 48 h with 10 nM BZ. The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant ( p

    Journal: Biochimica et biophysica acta

    Article Title: Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphoma

    doi: 10.1016/j.bbamcr.2014.07.012

    Figure Lengend Snippet: Bcl3 is highly expressed in CTCL cells, and its expression is inhibited by BZ. Western blotting of whole cell extracts prepared from CTCL Hut-78 (A) and HH cells (B) treated with increasing concentrations of BZ for 24 h, and analyzed by using Bcl3 antibody. To confirm equal protein loading, the membranes were stripped and re-probed with actin antibody. Each lane corresponds to approximately 5 × 10 4 cells. (C) Real time RT-PCR analysis of Bcl3 mRNA levels in Hut-78 cells treated 24 h with increasing BZ concentrations. (D) Real time RT-PCR analysis of Bcl3 mRNA levels in Hut-78 cells treated 0, 6, 24 and 48 h with 10 nM BZ. The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant ( p

    Article Snippet: The primers used for the quantification of Bcl3, cIAP1, cIAP2, Bcl2, IL-8, IL-17, and actin mRNA were purchased from SA Biosciences (Frederick, MD, USA).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR

    Suppression of Bcl3 inhibits expression of anti-apoptotic genes, but induces expression of pro-inflammatory genes in CTCL cells. (A) Real time RT-PCR analysis of cIAP1, cIAP2, Bcl2, IL-8 and IL-17 mRNA levels in Hut-78 cells transfected with control non-silencing siRNA or shRNA (full columns), and Bcl3 specific siRNA (empty columns) or shRNA (gray columns). The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant ( p

    Journal: Biochimica et biophysica acta

    Article Title: Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphoma

    doi: 10.1016/j.bbamcr.2014.07.012

    Figure Lengend Snippet: Suppression of Bcl3 inhibits expression of anti-apoptotic genes, but induces expression of pro-inflammatory genes in CTCL cells. (A) Real time RT-PCR analysis of cIAP1, cIAP2, Bcl2, IL-8 and IL-17 mRNA levels in Hut-78 cells transfected with control non-silencing siRNA or shRNA (full columns), and Bcl3 specific siRNA (empty columns) or shRNA (gray columns). The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant ( p

    Article Snippet: The primers used for the quantification of Bcl3, cIAP1, cIAP2, Bcl2, IL-8, IL-17, and actin mRNA were purchased from SA Biosciences (Frederick, MD, USA).

    Techniques: Expressing, Quantitative RT-PCR, Transfection, shRNA

    The mRNA expression of IL - 17A with the mRNA expression of IL - 4 , cytokine IL-4 and SCS values among different genotypes in Sanhe Cattle. a Relative mRNA expression of 3 genotypes of SNP in IL - 17A gene. b Relative mRNA expression of 3 genotypes of SNP in IL - 17A in association with IL-4 gene. c Comparison of the genotypes with respect to SCS. d Comparison of the 3 genotypes with respect to the IL-4 cytokines

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle

    doi: 10.1186/s40104-016-0137-1

    Figure Lengend Snippet: The mRNA expression of IL - 17A with the mRNA expression of IL - 4 , cytokine IL-4 and SCS values among different genotypes in Sanhe Cattle. a Relative mRNA expression of 3 genotypes of SNP in IL - 17A gene. b Relative mRNA expression of 3 genotypes of SNP in IL - 17A in association with IL-4 gene. c Comparison of the genotypes with respect to SCS. d Comparison of the 3 genotypes with respect to the IL-4 cytokines

    Article Snippet: The AG genotype of IL -17A had lower mRNA expression and higher values of the other 3 indicators i.e. IL -4 gene, IL-4 cytokine and SCS.

    Techniques: Expressing

    The trends of mRNA expression of different genotypes of IL - 17A gene in Sanhe Cattle. a Trends of mRNA expression of different genotypes of IL - 17A gene with IL - 4 gene. b Trends of mRNA expression of different genotypes of IL - 17A gene with IL-4 cytokine. c Trends of mRNA expression of different genotypes of IL - 17A gene with SCS values

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle

    doi: 10.1186/s40104-016-0137-1

    Figure Lengend Snippet: The trends of mRNA expression of different genotypes of IL - 17A gene in Sanhe Cattle. a Trends of mRNA expression of different genotypes of IL - 17A gene with IL - 4 gene. b Trends of mRNA expression of different genotypes of IL - 17A gene with IL-4 cytokine. c Trends of mRNA expression of different genotypes of IL - 17A gene with SCS values

    Article Snippet: The AG genotype of IL -17A had lower mRNA expression and higher values of the other 3 indicators i.e. IL -4 gene, IL-4 cytokine and SCS.

    Techniques: Expressing