il-1β Search Results


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  • 95
    Thermo Fisher recombinant interleukin 1β il 1β
    Upregulation of FSTL1 expression induced by inflammatory mediators in fibroblast-like synoviocytes (FLSs) from patients with RA . (A) RA FLSs ( n = 6) were treated with the indicated stimuli for 24 hours or were left untreated (medium). FSTL1 mRNA was determined using RT-PCR. The results are presented in relative units, and the P values derived from paired t -tests are indicated for each comparison. (B) FLSs from two RA patients (RA1 and RA2) were stimulated by combined <t>IL-1β</t> and TNFα or by TGFβ, or they were left untreated for 36 hours or 72 hours. Cell lysates were collected and analyzed by Western blot analysis using the anti-FSTL1 polyclonal antibody. β-Actin was used as a loading control. FSTL1, follistatin-like protein 1; RA, rheumatoid arthritis; PCR, polymerase chain reaction; IL-1β, <t>interleukin-1β;</t> TNFα, tumor necrosis factor α; TGFβ, transforming growth factor β; LPS, lipopolysaccharide; bLP, bacterial lipoprotein; PIC, polyinosinic:polycytidylic acid.
    Recombinant Interleukin 1β Il 1β, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore interleukin 1β il 1β
    Upregulation of FSTL1 expression induced by inflammatory mediators in fibroblast-like synoviocytes (FLSs) from patients with RA . (A) RA FLSs ( n = 6) were treated with the indicated stimuli for 24 hours or were left untreated (medium). FSTL1 mRNA was determined using RT-PCR. The results are presented in relative units, and the P values derived from paired t -tests are indicated for each comparison. (B) FLSs from two RA patients (RA1 and RA2) were stimulated by combined <t>IL-1β</t> and TNFα or by TGFβ, or they were left untreated for 36 hours or 72 hours. Cell lysates were collected and analyzed by Western blot analysis using the anti-FSTL1 polyclonal antibody. β-Actin was used as a loading control. FSTL1, follistatin-like protein 1; RA, rheumatoid arthritis; PCR, polymerase chain reaction; IL-1β, <t>interleukin-1β;</t> TNFα, tumor necrosis factor α; TGFβ, transforming growth factor β; LPS, lipopolysaccharide; bLP, bacterial lipoprotein; PIC, polyinosinic:polycytidylic acid.
    Interleukin 1β Il 1β, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems interleukin 1β il 1β
    Mindin mRNA expression is upregulated by CpG-ODN stimulation. About 1 × 10 5 RAW 264.7 cells were cultured in 12-well plates and stimulated with cytokines of mouse recombinant tumor necrosis factor-α (TNF-α) and <t>interleukin-1β</t> (IL-1β), toll-like receptor (TLR) ligands of Pam3-CSK4, peptidoglycans (PGN), lipopolysaccharide (LPS), flagellin and CpG-ODN 1585 (B) for 8 h, then cells were harvested for RNA isolation and relative mRNA expression of mindin was analyzed using quantitative real time polymerase chain reaction (RT-PCR). In each group, bars represent mean ± SD; b P
    Interleukin 1β Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 89/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio interleukin 1β il 1β
    Mindin mRNA expression is upregulated by CpG-ODN stimulation. About 1 × 10 5 RAW 264.7 cells were cultured in 12-well plates and stimulated with cytokines of mouse recombinant tumor necrosis factor-α (TNF-α) and <t>interleukin-1β</t> (IL-1β), toll-like receptor (TLR) ligands of Pam3-CSK4, peptidoglycans (PGN), lipopolysaccharide (LPS), flagellin and CpG-ODN 1585 (B) for 8 h, then cells were harvested for RNA isolation and relative mRNA expression of mindin was analyzed using quantitative real time polymerase chain reaction (RT-PCR). In each group, bars represent mean ± SD; b P
    Interleukin 1β Il 1β, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    USCN Life interleukin 1β il 1β
    Mindin mRNA expression is upregulated by CpG-ODN stimulation. About 1 × 10 5 RAW 264.7 cells were cultured in 12-well plates and stimulated with cytokines of mouse recombinant tumor necrosis factor-α (TNF-α) and <t>interleukin-1β</t> (IL-1β), toll-like receptor (TLR) ligands of Pam3-CSK4, peptidoglycans (PGN), lipopolysaccharide (LPS), flagellin and CpG-ODN 1585 (B) for 8 h, then cells were harvested for RNA isolation and relative mRNA expression of mindin was analyzed using quantitative real time polymerase chain reaction (RT-PCR). In each group, bars represent mean ± SD; b P
    Interleukin 1β Il 1β, supplied by USCN Life, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher interleukin 1β il 1β
    Inflammasome proteins are elevated in the serum of stroke patients. (A) Protein levels in pg/ml of caspase-1 ASC, <t>IL-1β</t> and IL-18 analyze by with a Simple Plex system in serum samples from patients with stroke and healthy donors. p -value of significance is shown above each box plot. Box and whiskers are shown for the 5th and 95th percentile. N.S., Not Significant. Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. (B) ROC curves for caspase-1 (orange), ASC (blue), IL-1β (black) and IL-18 (green). Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. Dark circles correspond to data points outside the 95% confidence interval.
    Interleukin 1β Il 1β, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam interleukin 1β il 1β
    Inflammasome proteins are elevated in the serum of stroke patients. (A) Protein levels in pg/ml of caspase-1 ASC, <t>IL-1β</t> and IL-18 analyze by with a Simple Plex system in serum samples from patients with stroke and healthy donors. p -value of significance is shown above each box plot. Box and whiskers are shown for the 5th and 95th percentile. N.S., Not Significant. Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. (B) ROC curves for caspase-1 (orange), ASC (blue), IL-1β (black) and IL-18 (green). Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. Dark circles correspond to data points outside the 95% confidence interval.
    Interleukin 1β Il 1β, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems recombinant interleukin 1β il 1β
    Inflammasome proteins are elevated in the serum of stroke patients. (A) Protein levels in pg/ml of caspase-1 ASC, <t>IL-1β</t> and IL-18 analyze by with a Simple Plex system in serum samples from patients with stroke and healthy donors. p -value of significance is shown above each box plot. Box and whiskers are shown for the 5th and 95th percentile. N.S., Not Significant. Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. (B) ROC curves for caspase-1 (orange), ASC (blue), IL-1β (black) and IL-18 (green). Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. Dark circles correspond to data points outside the 95% confidence interval.
    Recombinant Interleukin 1β Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Quanterix il1β
    Concentrations of biomarkers for adults with DS with and without clinical dementia. a Aβ 40 , b Aβ 42 , c Aβ 42 /Aβ 40 , d Aβ 42 /t-tau, e t-tau, f <t>IL1β,</t> g IL10, h IL6, i TNFα, and j NfL. Lines indicate median value (pg/ml)
    Il1β, supplied by Quanterix, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioVision interleukin 1β il 1β
    Concentrations of biomarkers for adults with DS with and without clinical dementia. a Aβ 40 , b Aβ 42 , c Aβ 42 /Aβ 40 , d Aβ 42 /t-tau, e t-tau, f <t>IL1β,</t> g IL10, h IL6, i TNFα, and j NfL. Lines indicate median value (pg/ml)
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    Cell Signaling Technology Inc il 1β
    Lack of the −16kb enhancer of Fgf23 blunts the Tnfα‐ and <t>IL‐1β‐induced</t> increase in Fgf23 levels. Eight‐week‐old wild‐type (WT) and Fgf23 −16KO female mice were injected with 2 μg of recombinant Tnfα or vehicle, while their male littermates were injected with 50 ng/g of IL‐1β or PBS. The tissues were collected 3 hours after TNFα and 6 hours after IL‐1β injection. The Fgf23 mRNA levels were measured by RT‐PCR and represented here as the mean ± SD ( n = 5 to 9 mice/group). Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. * p
    Il 1β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems interleukin 1β il 1β elisa kit
    Lack of the −16kb enhancer of Fgf23 blunts the Tnfα‐ and <t>IL‐1β‐induced</t> increase in Fgf23 levels. Eight‐week‐old wild‐type (WT) and Fgf23 −16KO female mice were injected with 2 μg of recombinant Tnfα or vehicle, while their male littermates were injected with 50 ng/g of IL‐1β or PBS. The tissues were collected 3 hours after TNFα and 6 hours after IL‐1β injection. The Fgf23 mRNA levels were measured by RT‐PCR and represented here as the mean ± SD ( n = 5 to 9 mice/group). Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. * p
    Interleukin 1β Il 1β Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore interleukin 1β il 1β levels
    Lack of the −16kb enhancer of Fgf23 blunts the Tnfα‐ and <t>IL‐1β‐induced</t> increase in Fgf23 levels. Eight‐week‐old wild‐type (WT) and Fgf23 −16KO female mice were injected with 2 μg of recombinant Tnfα or vehicle, while their male littermates were injected with 50 ng/g of IL‐1β or PBS. The tissues were collected 3 hours after TNFα and 6 hours after IL‐1β injection. The Fgf23 mRNA levels were measured by RT‐PCR and represented here as the mean ± SD ( n = 5 to 9 mice/group). Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. * p
    Interleukin 1β Il 1β Levels, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abnova interleukin 1β il 1β
    The mRNA abundance of <t>interleukin-1β</t> (IL-1β ), tumor necrosis factor-α (TNF-α ), IL-8 and xanthine oxidoreductase ( XOR ) in udder of rats. The mRNA abundances of IL-1β , TNF-α , IL-8 and XOR were determined by RT-PCR with mammary tissues obtained after saline infusion at day 14 of gestation from pregnant rats ( n = 7) and age-matched non-pregnant rats ( n = 11). Values are presented as means ± SE. Differences between pregnant and non-pregnant rats were indicated by asterisks (**** P
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    R&D Systems recombinant human interleukin 1β il 1β
    The mRNA abundance of <t>interleukin-1β</t> (IL-1β ), tumor necrosis factor-α (TNF-α ), IL-8 and xanthine oxidoreductase ( XOR ) in udder of rats. The mRNA abundances of IL-1β , TNF-α , IL-8 and XOR were determined by RT-PCR with mammary tissues obtained after saline infusion at day 14 of gestation from pregnant rats ( n = 7) and age-matched non-pregnant rats ( n = 11). Values are presented as means ± SE. Differences between pregnant and non-pregnant rats were indicated by asterisks (**** P
    Recombinant Human Interleukin 1β Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti interleukin 1β il 1β
    The mRNA abundance of <t>interleukin-1β</t> (IL-1β ), tumor necrosis factor-α (TNF-α ), IL-8 and xanthine oxidoreductase ( XOR ) in udder of rats. The mRNA abundances of IL-1β , TNF-α , IL-8 and XOR were determined by RT-PCR with mammary tissues obtained after saline infusion at day 14 of gestation from pregnant rats ( n = 7) and age-matched non-pregnant rats ( n = 11). Values are presented as means ± SE. Differences between pregnant and non-pregnant rats were indicated by asterisks (**** P
    Anti Interleukin 1β Il 1β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ImmunoTools interleukin 1β il 1β
    The mRNA abundance of <t>interleukin-1β</t> (IL-1β ), tumor necrosis factor-α (TNF-α ), IL-8 and xanthine oxidoreductase ( XOR ) in udder of rats. The mRNA abundances of IL-1β , TNF-α , IL-8 and XOR were determined by RT-PCR with mammary tissues obtained after saline infusion at day 14 of gestation from pregnant rats ( n = 7) and age-matched non-pregnant rats ( n = 11). Values are presented as means ± SE. Differences between pregnant and non-pregnant rats were indicated by asterisks (**** P
    Interleukin 1β Il 1β, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PeproTech il 1β
    Ozone suppressed inflammatory cytokines and regulated chondrocyte metabolism, and this effect was blocked by com C. Notes: The cells were treated with <t>IL-1β</t> for 24 hours, with or without pretreatment with the AMPK inhibitor com C (5 µM) for 12 hours. The mRNA expression of IL-6 ( A ), TNF-α ( B ), MMP-13 ( C ) and TIMP-1 ( D ) in chondrocytes stimulated with IL-1β was measured by qRT-PCR. The data are presented as the mean ± SD of three independent experiments. * P
    Il 1β, supplied by PeproTech, used in various techniques. Bioz Stars score: 93/100, based on 3872 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems interleukin 1β il 1β elisa kits
    <t>Interleukin-1β</t> level in colon Values are mean ± SEM. a Significantly different from sham group at p
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    Thermo Fisher interleukin 1β il 1β elisa kits
    <t>Interleukin-1β</t> level in colon Values are mean ± SEM. a Significantly different from sham group at p
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    Keygen Biotech interleukin 1β il 1β
    <t>Interleukin-1β</t> level in colon Values are mean ± SEM. a Significantly different from sham group at p
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    Hornung interleukin 1β il 1β
    <t>Interleukin-1β</t> level in colon Values are mean ± SEM. a Significantly different from sham group at p
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    Cell Signaling Technology Inc il 1β antibody
    PmpG-1-vaults activate the NLRP3 inflammasome and induce <t>IL-1β</t> secretion, as measured by an ELISA assay
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    Image Search Results


    Upregulation of FSTL1 expression induced by inflammatory mediators in fibroblast-like synoviocytes (FLSs) from patients with RA . (A) RA FLSs ( n = 6) were treated with the indicated stimuli for 24 hours or were left untreated (medium). FSTL1 mRNA was determined using RT-PCR. The results are presented in relative units, and the P values derived from paired t -tests are indicated for each comparison. (B) FLSs from two RA patients (RA1 and RA2) were stimulated by combined IL-1β and TNFα or by TGFβ, or they were left untreated for 36 hours or 72 hours. Cell lysates were collected and analyzed by Western blot analysis using the anti-FSTL1 polyclonal antibody. β-Actin was used as a loading control. FSTL1, follistatin-like protein 1; RA, rheumatoid arthritis; PCR, polymerase chain reaction; IL-1β, interleukin-1β; TNFα, tumor necrosis factor α; TGFβ, transforming growth factor β; LPS, lipopolysaccharide; bLP, bacterial lipoprotein; PIC, polyinosinic:polycytidylic acid.

    Journal: Arthritis Research & Therapy

    Article Title: Follistatin-like protein 1 is elevated in systemic autoimmune diseases and correlated with disease activity in patients with rheumatoid arthritis

    doi: 10.1186/ar3241

    Figure Lengend Snippet: Upregulation of FSTL1 expression induced by inflammatory mediators in fibroblast-like synoviocytes (FLSs) from patients with RA . (A) RA FLSs ( n = 6) were treated with the indicated stimuli for 24 hours or were left untreated (medium). FSTL1 mRNA was determined using RT-PCR. The results are presented in relative units, and the P values derived from paired t -tests are indicated for each comparison. (B) FLSs from two RA patients (RA1 and RA2) were stimulated by combined IL-1β and TNFα or by TGFβ, or they were left untreated for 36 hours or 72 hours. Cell lysates were collected and analyzed by Western blot analysis using the anti-FSTL1 polyclonal antibody. β-Actin was used as a loading control. FSTL1, follistatin-like protein 1; RA, rheumatoid arthritis; PCR, polymerase chain reaction; IL-1β, interleukin-1β; TNFα, tumor necrosis factor α; TGFβ, transforming growth factor β; LPS, lipopolysaccharide; bLP, bacterial lipoprotein; PIC, polyinosinic:polycytidylic acid.

    Article Snippet: Cultures were stimulated for 24 hours for mRNA extraction or for 36 hours for protein collection with the following agents: 20 μg/ml polyinosinic:polycytidylic acid (InvivoGen, San Diego, CA, USA), 100 ng/ml lipopolysaccharide from Escherichia coli (Sigma-Aldrich), 300 ng/ml bacterial lipoprotein (InvivoGen), 10 ng/ml recombinant interleukin-1β (IL-1β) (Invitrogen, Carlsbad, CA, USA), 10 ng/ml tumor necrosis factor α (TNFα) (Invitrogen) and 10 ng/ml transforming factor β (TGFβ) (Invitrogen).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Western Blot, Polymerase Chain Reaction

    Mindin mRNA expression is upregulated by CpG-ODN stimulation. About 1 × 10 5 RAW 264.7 cells were cultured in 12-well plates and stimulated with cytokines of mouse recombinant tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), toll-like receptor (TLR) ligands of Pam3-CSK4, peptidoglycans (PGN), lipopolysaccharide (LPS), flagellin and CpG-ODN 1585 (B) for 8 h, then cells were harvested for RNA isolation and relative mRNA expression of mindin was analyzed using quantitative real time polymerase chain reaction (RT-PCR). In each group, bars represent mean ± SD; b P

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Mindin is upregulated during colitis and may activate NF-?B in a TLR-9 mediated manner

    doi: 10.3748/wjg.v16.i9.1070

    Figure Lengend Snippet: Mindin mRNA expression is upregulated by CpG-ODN stimulation. About 1 × 10 5 RAW 264.7 cells were cultured in 12-well plates and stimulated with cytokines of mouse recombinant tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), toll-like receptor (TLR) ligands of Pam3-CSK4, peptidoglycans (PGN), lipopolysaccharide (LPS), flagellin and CpG-ODN 1585 (B) for 8 h, then cells were harvested for RNA isolation and relative mRNA expression of mindin was analyzed using quantitative real time polymerase chain reaction (RT-PCR). In each group, bars represent mean ± SD; b P

    Article Snippet: About 1 × 105 of RAW 264.7 and CMT93 cells were cultured in 12-well plates and stimulated with the cytokines; 10 ng/mL of mouse recombinant tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) (both from R & D Systems); and different TLR ligands consisting of 0.05 μg/mL of Pam3-CSK4, 5 μg/mL of peptidoglycans (PGN), 0.1 μg/mL of LPS, 0.5 μg/mL of flagellin ( S. typhimurium ) and 5 μg/mL of CpG-ODN 1585 (all of the ligands were purchased from Invivogen), for 8 h, then cells were harvested for RNA isolation.

    Techniques: Expressing, Cell Culture, Recombinant, Isolation, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    Inflammasome proteins are elevated in the serum of stroke patients. (A) Protein levels in pg/ml of caspase-1 ASC, IL-1β and IL-18 analyze by with a Simple Plex system in serum samples from patients with stroke and healthy donors. p -value of significance is shown above each box plot. Box and whiskers are shown for the 5th and 95th percentile. N.S., Not Significant. Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. (B) ROC curves for caspase-1 (orange), ASC (blue), IL-1β (black) and IL-18 (green). Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. Dark circles correspond to data points outside the 95% confidence interval.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke

    doi: 10.3389/fnmol.2018.00309

    Figure Lengend Snippet: Inflammasome proteins are elevated in the serum of stroke patients. (A) Protein levels in pg/ml of caspase-1 ASC, IL-1β and IL-18 analyze by with a Simple Plex system in serum samples from patients with stroke and healthy donors. p -value of significance is shown above each box plot. Box and whiskers are shown for the 5th and 95th percentile. N.S., Not Significant. Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. (B) ROC curves for caspase-1 (orange), ASC (blue), IL-1β (black) and IL-18 (green). Caspase-1: N = 8 control and N = 13 stroke; ASC: N = 75 control and N = 16 stroke; IL-1: N = 9 control and N = 8 stroke; and IL-18: N = 79 control and N = 15 stroke. Dark circles correspond to data points outside the 95% confidence interval.

    Article Snippet: However, the ExoQuick method was able to isolate EV with higher levels of IL-1β than the Invitrogen method (Supplementary Figure ).

    Techniques:

    ASC is elevated in serum-derived EV of stroke patients. Protein levels in pg/ml of ASC (A) , IL-1β (B) and IL-18 (C) in serum-derived EV from patients with stroke and healthy donors. p -value of significance is shown above each box plot. Box and whiskers are shown for the 5th and 95th percentile. N.S., Not Significant. ASC: N = 16 control and 16 stroke; IL-1β: N = 10 control and 9 stroke; and IL-18: N = 16 control and 13 stroke. (D) ROC curves for ASC (blue), IL-1β (black) and IL-18 (green). ASC: N = 16 control and 16 stroke; IL-1β: N = 10 control and 9 stroke; and IL-18: N = 16 control and 13 stroke.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke

    doi: 10.3389/fnmol.2018.00309

    Figure Lengend Snippet: ASC is elevated in serum-derived EV of stroke patients. Protein levels in pg/ml of ASC (A) , IL-1β (B) and IL-18 (C) in serum-derived EV from patients with stroke and healthy donors. p -value of significance is shown above each box plot. Box and whiskers are shown for the 5th and 95th percentile. N.S., Not Significant. ASC: N = 16 control and 16 stroke; IL-1β: N = 10 control and 9 stroke; and IL-18: N = 16 control and 13 stroke. (D) ROC curves for ASC (blue), IL-1β (black) and IL-18 (green). ASC: N = 16 control and 16 stroke; IL-1β: N = 10 control and 9 stroke; and IL-18: N = 16 control and 13 stroke.

    Article Snippet: However, the ExoQuick method was able to isolate EV with higher levels of IL-1β than the Invitrogen method (Supplementary Figure ).

    Techniques: Derivative Assay

    Concentrations of biomarkers for adults with DS with and without clinical dementia. a Aβ 40 , b Aβ 42 , c Aβ 42 /Aβ 40 , d Aβ 42 /t-tau, e t-tau, f IL1β, g IL10, h IL6, i TNFα, and j NfL. Lines indicate median value (pg/ml)

    Journal: Alzheimer's Research & Therapy

    Article Title: Plasma biomarkers for amyloid, tau, and cytokines in Down syndrome and sporadic Alzheimer’s disease

    doi: 10.1186/s13195-019-0477-0

    Figure Lengend Snippet: Concentrations of biomarkers for adults with DS with and without clinical dementia. a Aβ 40 , b Aβ 42 , c Aβ 42 /Aβ 40 , d Aβ 42 /t-tau, e t-tau, f IL1β, g IL10, h IL6, i TNFα, and j NfL. Lines indicate median value (pg/ml)

    Article Snippet: For all three groups, plasma concentrations of Aβ40 , Aβ42 , and t-tau (Human Neurology 3-Plex A assay (N3PA)), IL1β (Human IL-1β 2.0), and IL10, IL6, and TNFα (Human Cytokine 3-Plex A) were measured in duplicate using ultrasensitive Simoa immunoassays (Quanterix, Lexington, MA, USA) according to the manufacturer’s instructions at the Institute of Psychiatry, Psychology and Neuroscience, King’s College London.

    Techniques:

    Relationships between biomarkers for adults with DS. a Log Aβ 42 and log t-tau, b log t-tau and log IL1β, and c log IL1β and log IL10

    Journal: Alzheimer's Research & Therapy

    Article Title: Plasma biomarkers for amyloid, tau, and cytokines in Down syndrome and sporadic Alzheimer’s disease

    doi: 10.1186/s13195-019-0477-0

    Figure Lengend Snippet: Relationships between biomarkers for adults with DS. a Log Aβ 42 and log t-tau, b log t-tau and log IL1β, and c log IL1β and log IL10

    Article Snippet: For all three groups, plasma concentrations of Aβ40 , Aβ42 , and t-tau (Human Neurology 3-Plex A assay (N3PA)), IL1β (Human IL-1β 2.0), and IL10, IL6, and TNFα (Human Cytokine 3-Plex A) were measured in duplicate using ultrasensitive Simoa immunoassays (Quanterix, Lexington, MA, USA) according to the manufacturer’s instructions at the Institute of Psychiatry, Psychology and Neuroscience, King’s College London.

    Techniques:

    Concentrations of biomarkers for each group. a Aβ 40 , b Aβ 42 , c Aβ 42 /Aβ 40 , d Aβ 42 /t-tau, e t-tau, f IL1β, g IL10, h IL6, and i TNFα. Lines indicate median value (pg/ml)

    Journal: Alzheimer's Research & Therapy

    Article Title: Plasma biomarkers for amyloid, tau, and cytokines in Down syndrome and sporadic Alzheimer’s disease

    doi: 10.1186/s13195-019-0477-0

    Figure Lengend Snippet: Concentrations of biomarkers for each group. a Aβ 40 , b Aβ 42 , c Aβ 42 /Aβ 40 , d Aβ 42 /t-tau, e t-tau, f IL1β, g IL10, h IL6, and i TNFα. Lines indicate median value (pg/ml)

    Article Snippet: For all three groups, plasma concentrations of Aβ40 , Aβ42 , and t-tau (Human Neurology 3-Plex A assay (N3PA)), IL1β (Human IL-1β 2.0), and IL10, IL6, and TNFα (Human Cytokine 3-Plex A) were measured in duplicate using ultrasensitive Simoa immunoassays (Quanterix, Lexington, MA, USA) according to the manufacturer’s instructions at the Institute of Psychiatry, Psychology and Neuroscience, King’s College London.

    Techniques:

    Lack of the −16kb enhancer of Fgf23 blunts the Tnfα‐ and IL‐1β‐induced increase in Fgf23 levels. Eight‐week‐old wild‐type (WT) and Fgf23 −16KO female mice were injected with 2 μg of recombinant Tnfα or vehicle, while their male littermates were injected with 50 ng/g of IL‐1β or PBS. The tissues were collected 3 hours after TNFα and 6 hours after IL‐1β injection. The Fgf23 mRNA levels were measured by RT‐PCR and represented here as the mean ± SD ( n = 5 to 9 mice/group). Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. * p

    Journal: JBMR Plus

    Article Title: A Novel Distal Enhancer Mediates Inflammation‐, PTH‐, and Early Onset Murine Kidney Disease‐Induced Expression of the Mouse Fgf23 Gene

    doi: 10.1002/jbm4.10023

    Figure Lengend Snippet: Lack of the −16kb enhancer of Fgf23 blunts the Tnfα‐ and IL‐1β‐induced increase in Fgf23 levels. Eight‐week‐old wild‐type (WT) and Fgf23 −16KO female mice were injected with 2 μg of recombinant Tnfα or vehicle, while their male littermates were injected with 50 ng/g of IL‐1β or PBS. The tissues were collected 3 hours after TNFα and 6 hours after IL‐1β injection. The Fgf23 mRNA levels were measured by RT‐PCR and represented here as the mean ± SD ( n = 5 to 9 mice/group). Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. * p

    Article Snippet: Reagents The following reagents were used for in vivo injections: LPS (Sigma, St. Louis, MO, USA), IL‐1β (Cell Signaling Technology, Danvers, MA, USA), and TNFα (R & D Systems, Minneapolis, MN, USA).

    Techniques: Mouse Assay, Injection, Recombinant, Reverse Transcription Polymerase Chain Reaction

    Inflammation increases Fgf23 mRNA expression in bone and non‐osseous tissues. Twelve‐month‐old female mice were injected intraperitoneally (ip) with 10 mg/kg of LPS or PBS. Eight‐week‐old female mice were injected with 2 μg of recombinant TNFα, 50 ng/g of IL‐1β, or PBS. The tissues and blood were collected 3 hours after TNFα and 6 hours after LPS or IL‐1β injection. ( A–H ) Fgf23 mRNA levels were measured by quantitative RT‐PCR and normalized to beta‐actin mRNA levels. ( I ) Blood was collected by cardiac puncture, and circulating intact FGF23 (iFGF23) levels were measured in EDTA plasma. ( A–I ) All values represent mean ± SD of 3 to 4 mice/group. All statistical comparisons were performed using Student's t test. * p

    Journal: JBMR Plus

    Article Title: A Novel Distal Enhancer Mediates Inflammation‐, PTH‐, and Early Onset Murine Kidney Disease‐Induced Expression of the Mouse Fgf23 Gene

    doi: 10.1002/jbm4.10023

    Figure Lengend Snippet: Inflammation increases Fgf23 mRNA expression in bone and non‐osseous tissues. Twelve‐month‐old female mice were injected intraperitoneally (ip) with 10 mg/kg of LPS or PBS. Eight‐week‐old female mice were injected with 2 μg of recombinant TNFα, 50 ng/g of IL‐1β, or PBS. The tissues and blood were collected 3 hours after TNFα and 6 hours after LPS or IL‐1β injection. ( A–H ) Fgf23 mRNA levels were measured by quantitative RT‐PCR and normalized to beta‐actin mRNA levels. ( I ) Blood was collected by cardiac puncture, and circulating intact FGF23 (iFGF23) levels were measured in EDTA plasma. ( A–I ) All values represent mean ± SD of 3 to 4 mice/group. All statistical comparisons were performed using Student's t test. * p

    Article Snippet: Reagents The following reagents were used for in vivo injections: LPS (Sigma, St. Louis, MO, USA), IL‐1β (Cell Signaling Technology, Danvers, MA, USA), and TNFα (R & D Systems, Minneapolis, MN, USA).

    Techniques: Expressing, Mouse Assay, Injection, Recombinant, Quantitative RT-PCR

    The −16kb enhancer of Fgf23 mediates the inflammation‐induced increase in circulating intact FGF23 levels. Eight‐ to 12‐week‐old wild‐type (WT) and Fgf23 −16KO mice were injected with 10 mg/kg of LPS (female mice) ( A ), 2 μg of recombinant TNFα (female mice) ( B ), 50 ng/g of IL‐1β (male mice) ( C ), or vehicle. Cardiac blood was collected at time of death, which was 6 hours after LPS and IL‐1β injection and 3 hours after TNFα injection. Circulating intact FGF23 (iFGF23) levels were measured by ELISA. The bars represent the mean ± SD of 4 to 9 mice/group. Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. * p

    Journal: JBMR Plus

    Article Title: A Novel Distal Enhancer Mediates Inflammation‐, PTH‐, and Early Onset Murine Kidney Disease‐Induced Expression of the Mouse Fgf23 Gene

    doi: 10.1002/jbm4.10023

    Figure Lengend Snippet: The −16kb enhancer of Fgf23 mediates the inflammation‐induced increase in circulating intact FGF23 levels. Eight‐ to 12‐week‐old wild‐type (WT) and Fgf23 −16KO mice were injected with 10 mg/kg of LPS (female mice) ( A ), 2 μg of recombinant TNFα (female mice) ( B ), 50 ng/g of IL‐1β (male mice) ( C ), or vehicle. Cardiac blood was collected at time of death, which was 6 hours after LPS and IL‐1β injection and 3 hours after TNFα injection. Circulating intact FGF23 (iFGF23) levels were measured by ELISA. The bars represent the mean ± SD of 4 to 9 mice/group. Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. * p

    Article Snippet: Reagents The following reagents were used for in vivo injections: LPS (Sigma, St. Louis, MO, USA), IL‐1β (Cell Signaling Technology, Danvers, MA, USA), and TNFα (R & D Systems, Minneapolis, MN, USA).

    Techniques: Mouse Assay, Injection, Recombinant, Enzyme-linked Immunosorbent Assay

    The mRNA abundance of interleukin-1β (IL-1β ), tumor necrosis factor-α (TNF-α ), IL-8 and xanthine oxidoreductase ( XOR ) in udder of rats. The mRNA abundances of IL-1β , TNF-α , IL-8 and XOR were determined by RT-PCR with mammary tissues obtained after saline infusion at day 14 of gestation from pregnant rats ( n = 7) and age-matched non-pregnant rats ( n = 11). Values are presented as means ± SE. Differences between pregnant and non-pregnant rats were indicated by asterisks (**** P

    Journal: Animal Nutrition

    Article Title: Reproductive stage associated changes in plasma fatty acid profile and proinflammatory cytokine expression in rat mammary glands

    doi: 10.1016/j.aninu.2016.03.008

    Figure Lengend Snippet: The mRNA abundance of interleukin-1β (IL-1β ), tumor necrosis factor-α (TNF-α ), IL-8 and xanthine oxidoreductase ( XOR ) in udder of rats. The mRNA abundances of IL-1β , TNF-α , IL-8 and XOR were determined by RT-PCR with mammary tissues obtained after saline infusion at day 14 of gestation from pregnant rats ( n = 7) and age-matched non-pregnant rats ( n = 11). Values are presented as means ± SE. Differences between pregnant and non-pregnant rats were indicated by asterisks (**** P

    Article Snippet: 2.7 Immunohistochemistry Polyclonal antibodies combined with the avidin-biotinperoxidase complex technique were used for the immunohistochemical detection of interleukin-1β (IL-1β) (Abnova, Walnut, CA, USA) and tumor necrosis factor-α (TNF-α) (Novus, SaintCharles, MO, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Immunohistochemical localization of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in mammary glands of the rats at different reproductive stages. Mammary glands were collected after saline infusion from non-pregnant rats that designated as rats at day 1 of gestation ( n = 6), pregnant rats that was infused by saline at day 14 of gestation ( n = 5) and day 3 postpartum ( n = 5). (A) Interleukin-1β and TNF-α production is presented as the average percentage of the positively stained areas. The microphotograph from one rat with the positive primary IL-1β or TNF-α antibody was visualized with diaminobenzidine reaction. The area positive for (B) IL-1β and (C) TNF-α in mammary tissue of rats at different reproductive stages was quantified by Easy Image 3000 software at a magnification of 400×. Values are presented as means ± SE. Different letters signify statistical difference ( P

    Journal: Animal Nutrition

    Article Title: Reproductive stage associated changes in plasma fatty acid profile and proinflammatory cytokine expression in rat mammary glands

    doi: 10.1016/j.aninu.2016.03.008

    Figure Lengend Snippet: Immunohistochemical localization of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in mammary glands of the rats at different reproductive stages. Mammary glands were collected after saline infusion from non-pregnant rats that designated as rats at day 1 of gestation ( n = 6), pregnant rats that was infused by saline at day 14 of gestation ( n = 5) and day 3 postpartum ( n = 5). (A) Interleukin-1β and TNF-α production is presented as the average percentage of the positively stained areas. The microphotograph from one rat with the positive primary IL-1β or TNF-α antibody was visualized with diaminobenzidine reaction. The area positive for (B) IL-1β and (C) TNF-α in mammary tissue of rats at different reproductive stages was quantified by Easy Image 3000 software at a magnification of 400×. Values are presented as means ± SE. Different letters signify statistical difference ( P

    Article Snippet: 2.7 Immunohistochemistry Polyclonal antibodies combined with the avidin-biotinperoxidase complex technique were used for the immunohistochemical detection of interleukin-1β (IL-1β) (Abnova, Walnut, CA, USA) and tumor necrosis factor-α (TNF-α) (Novus, SaintCharles, MO, USA).

    Techniques: Immunohistochemistry, Staining, Software

    Ozone suppressed inflammatory cytokines and regulated chondrocyte metabolism, and this effect was blocked by com C. Notes: The cells were treated with IL-1β for 24 hours, with or without pretreatment with the AMPK inhibitor com C (5 µM) for 12 hours. The mRNA expression of IL-6 ( A ), TNF-α ( B ), MMP-13 ( C ) and TIMP-1 ( D ) in chondrocytes stimulated with IL-1β was measured by qRT-PCR. The data are presented as the mean ± SD of three independent experiments. * P

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: Ozone suppressed inflammatory cytokines and regulated chondrocyte metabolism, and this effect was blocked by com C. Notes: The cells were treated with IL-1β for 24 hours, with or without pretreatment with the AMPK inhibitor com C (5 µM) for 12 hours. The mRNA expression of IL-6 ( A ), TNF-α ( B ), MMP-13 ( C ) and TIMP-1 ( D ) in chondrocytes stimulated with IL-1β was measured by qRT-PCR. The data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques: Expressing, Quantitative RT-PCR

    Ozone increased the autophagosomes in chondrocytes stimulated with IL-1β (10 ng/mL), which was blocked by 3MA and com C. Notes: The arrows indicate autophagosomes. The data are from three independent experiments.

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: Ozone increased the autophagosomes in chondrocytes stimulated with IL-1β (10 ng/mL), which was blocked by 3MA and com C. Notes: The arrows indicate autophagosomes. The data are from three independent experiments.

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques:

    Ozone activated the AMPK/mTOR signaling pathway in chondrocytes stimulated with IL-1β, and this effect was inhibited by com C. Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the AMPK inhibitor com C (5 µM) for 12 hours. The levels of p-AMPK ( A ), p-mTOR ( B ) and ULK1 ( C ) and the Bcl2/Bax ratio ( D ) in chondrocytes stimulated with IL-1β were measured by Western blotting. The data are presented as the mean ± SD of three independent experiments. * P

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: Ozone activated the AMPK/mTOR signaling pathway in chondrocytes stimulated with IL-1β, and this effect was inhibited by com C. Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the AMPK inhibitor com C (5 µM) for 12 hours. The levels of p-AMPK ( A ), p-mTOR ( B ) and ULK1 ( C ) and the Bcl2/Bax ratio ( D ) in chondrocytes stimulated with IL-1β were measured by Western blotting. The data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques: Western Blot

    The increased cell viability in chondrocytes stimulated with IL-1β and treated with ozone was reduced by 3MA or com C. Notes: ( A ) Chondrocytes stimulated with IL-1β (10 ng/mL) for 24 hours were treated with different doses of ozone for different time periods, and treatment with 30 µg/mL ozone for 30 minutes promoted cell viability in chondrocytes stimulated with IL-1β, as determined by the CCK-8 assay. ( B ) Chondrocytes stimulated with IL-1β (10 ng/mL) for 24 hours were treated with ozone (30 µg/mL) in the absence or presence of 3MA (100 nM), and the improvement in cell viability induced by ozone was abated by 3MA. ( C ) Chondrocytes stimulated with IL-1β (10 ng/mL) for 24 hours were treated with ozone (30 µg/mL) in the absence or presence of com C (5 µM), and the improvement in cell viability induced by ozone was abated by com C. The data are presented as the mean ± SD for three independent experiments. * P

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: The increased cell viability in chondrocytes stimulated with IL-1β and treated with ozone was reduced by 3MA or com C. Notes: ( A ) Chondrocytes stimulated with IL-1β (10 ng/mL) for 24 hours were treated with different doses of ozone for different time periods, and treatment with 30 µg/mL ozone for 30 minutes promoted cell viability in chondrocytes stimulated with IL-1β, as determined by the CCK-8 assay. ( B ) Chondrocytes stimulated with IL-1β (10 ng/mL) for 24 hours were treated with ozone (30 µg/mL) in the absence or presence of 3MA (100 nM), and the improvement in cell viability induced by ozone was abated by 3MA. ( C ) Chondrocytes stimulated with IL-1β (10 ng/mL) for 24 hours were treated with ozone (30 µg/mL) in the absence or presence of com C (5 µM), and the improvement in cell viability induced by ozone was abated by com C. The data are presented as the mean ± SD for three independent experiments. * P

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques: CCK-8 Assay

    Ozone induced autophagy in chondrocytes stimulated with IL-1β (10 ng/mL), and this effect was blocked by com C. Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the AMPK inhibitor com C (5 µM) for12 h. The levels of LC3 ( A, B ) and P62 ( C ) were measured by Western blotting and immunofluorescence. The data are presented as the mean ± SD of three independent experiments. * P

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: Ozone induced autophagy in chondrocytes stimulated with IL-1β (10 ng/mL), and this effect was blocked by com C. Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the AMPK inhibitor com C (5 µM) for12 h. The levels of LC3 ( A, B ) and P62 ( C ) were measured by Western blotting and immunofluorescence. The data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques: Western Blot, Immunofluorescence

    Ozone activated the AMPK/mTOR signaling pathway in chondrocytes stimulated with IL-1β (10 ng/mL), and this effect was inhibited by 3MA. Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the autophagy inhibitor 3MA (100 nM) for 12 hours. The levels of p-AMPK ( A ), p-mTOR ( B ) and Beclin-1 ( C ) in chondrocytes stimulated with IL-1β were measured by Western blotting. The data are presented as the mean ± SD of three independent experiments. * P

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: Ozone activated the AMPK/mTOR signaling pathway in chondrocytes stimulated with IL-1β (10 ng/mL), and this effect was inhibited by 3MA. Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the autophagy inhibitor 3MA (100 nM) for 12 hours. The levels of p-AMPK ( A ), p-mTOR ( B ) and Beclin-1 ( C ) in chondrocytes stimulated with IL-1β were measured by Western blotting. The data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques: Western Blot

    Ozone suppresses inflammatory cytokines in chondrocytes stimulated with IL-1β (10 ng/mL). Notes: Cells were treated with IL-1β (10 ng/mL) for 24 hours, with or without pretreatment with the autophagy inhibitor 3MA (100 nM) for 12 hours. The mRNA expression of IL-6 ( A ) and TNFα ( B ) in chondrocytes stimulated with IL-1β was measured by qRT-PCR. The data are presented as the mean ± SD of three independent experiments. * P

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: Ozone suppresses inflammatory cytokines in chondrocytes stimulated with IL-1β (10 ng/mL). Notes: Cells were treated with IL-1β (10 ng/mL) for 24 hours, with or without pretreatment with the autophagy inhibitor 3MA (100 nM) for 12 hours. The mRNA expression of IL-6 ( A ) and TNFα ( B ) in chondrocytes stimulated with IL-1β was measured by qRT-PCR. The data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques: Expressing, Quantitative RT-PCR

    Role of ozone-induced autophagy in chondrocytes stimulated with IL-1β (10 ng/mL). Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the autophagy inhibitor 3MA (100 nM) for 12 hours. The levels of LC3 II ( A, C ) and P62 ( B, D ) were measured by Western blotting and immunofluorescence. The data are presented as the mean ± SD of three independent experiments.

    Journal: Journal of Pain Research

    Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway

    doi: 10.2147/JPR.S183594

    Figure Lengend Snippet: Role of ozone-induced autophagy in chondrocytes stimulated with IL-1β (10 ng/mL). Notes: Cells were treated with IL-1β for 24 hours, with or without pretreatment with the autophagy inhibitor 3MA (100 nM) for 12 hours. The levels of LC3 II ( A, C ) and P62 ( B, D ) were measured by Western blotting and immunofluorescence. The data are presented as the mean ± SD of three independent experiments.

    Article Snippet: Osteoarthritis chondrocyte model Cells were stimulated with IL-1β (10 ng/mL) (PeproTech, USA) for 24 hours before O3 treatment similar to OA chondrocytes.

    Techniques: Western Blot, Immunofluorescence

    Interleukin-1β level in colon Values are mean ± SEM. a Significantly different from sham group at p

    Journal: Archives of Medical Science : AMS

    Article Title: On the benefit of galls of Quercus brantii Lindl. in murine colitis: the role of free gallic acid

    doi: 10.5114/aoms.2014.47831

    Figure Lengend Snippet: Interleukin-1β level in colon Values are mean ± SEM. a Significantly different from sham group at p

    Article Snippet: Chemicals 2,4,6-Trinitrobenzene sulfonic acid (TNBS) and rhodanine from Sigma-Aldrich Chemie (GmbH, Munich, Germany), thiobarbituric acid (TBA), trichloroacetic acid (TCA), n-butanol, hexadecyl trimethyl ammonium bromide (HETAB), 2,4,6-tri (2-pyridyl)-s-triazine (TPTZ), diphenyl-2-picryl hydrazyl (DPPH), methanol, butyl hydroxy anisol (BHA), hydrochloric acid (HCl), malondialdehyde (MDA), ethylenediamine tetra-acetic acid (EDTA), O-dianisidine hydrochloride, hydrogen peroxide, acetic acid, sodium acetate, Coomassie reagent, bovine serum albumin (BSA), ferric chloride (FeCl3 • 6 H2 O), sodium sulfate (Na2 SO4 ), sulfuric acid (H2 SO4 ), phosphoric acid (H3 PO4 ), potassium dihydrogen phosphate (KH2 PO4 ), potassium hydrogen diphosphate (K2 HPO4 ), peroxide hydrogen (H2 O2 ), sodium carbonate (Na2 CO3 ), Na-K-tartrate, cupric sulfate (CuSO4 • 5 H2 O), Folin-Ciocalteu reagent and Gallic acid from Merck (Germany), rat-specific tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) ELISA kits from Bender MedSystems (Austria), and dexamethasone and vitamin E from the local Pharmaceutical Co. (Tehran) were used in this study.

    Techniques:

    PmpG-1-vaults activate the NLRP3 inflammasome and induce IL-1β secretion, as measured by an ELISA assay

    Journal: Vaccine

    Article Title: Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope

    doi: 10.1016/j.vaccine.2014.11.028

    Figure Lengend Snippet: PmpG-1-vaults activate the NLRP3 inflammasome and induce IL-1β secretion, as measured by an ELISA assay

    Article Snippet: To detect mature IL-1β, the blot was probed with IL-1β antibody (Cell Signaling) at a 1:1000 dilution, and then incubated again with 1:10000 dilution of anti-mouse secondary antibody (Santa Cruz Biotechnology).

    Techniques: Enzyme-linked Immunosorbent Assay