Journal: Cell Research
Article Title: Identification of IFN-?-producing innate B cells
Figure Lengend Snippet: CD11a hi FcγRIII hi B cells produce a high level of IFN-γ in response to CD40 ligation. C57BL/6 mice were infected with 2 × 10 6 LM. (A) The intracellular expression of IL-1α, IL-2, IL-6, IL-10 and IFN-γ in CD11a hi FcγRIII hi and conventional B cells was assayed on day 3 post-infection. (B - G) Splenic CD11a hi FcγRIII hi and conventional B cells were sorted on day 3 post-infection. IL-1β, IL-2, IL-6, IL-12p70, IFN-γ, or TNF-α secretion by CD11a hi FcγRIII hi and conventional B cells was detected by ELISA. Data shown represent the mean ± SD of triplicate experiments. * P
Article Snippet: Fluorescence-conjugated mAbs against CD4 (RM4-5), CD5 (53-7.3), CD8 (53-6.7), CD11a (2D7), CD11b (M1/70), CD11c (HL3), CD16/32 (2.4G2), CD19 (1D3), CD21/CD35 (7G6), CD23 (B3B4), CD40 (3/23), CD40L (TRAP1), CD43 (S7), CD45R/B220 (RA3-6B2), CD80 (16-10A1), CD86 (GL1), CD93 (C1qRp) (R139), F4/80 (6F12), NK1.1 (PK136), I-A/I-E (2G9), IgD (11-26c.2a), IgM (G155-228), IL-1α (364-3B3-14), IL-2 (MQ1-17H12), IL-6 (MP5-20F3), IL-10 (JES3-19F1), IFN-γ (B27), IgD (11-26c.2a) and IgM (G155-228) and agonistic anti-CD40 (HM40-3) and neutralizing CD11a mAbs (M17/4) were purchased from BD Biosciences Pharmingen (San Diego, CA, USA).
Techniques: Ligation, Mouse Assay, Infection, Expressing, Enzyme-linked Immunosorbent Assay