Journal: The Korean Journal of Parasitology

Article Title: Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis

doi: 10.3347/kjp.2014.52.2.137

Figure Lengend Snippet: Results of IgM ELISA with recombinant SAG1, SAG2, and SAG3 in human sera. P, IgM positive; N, IgM negative. Cut-off value; 0.60 (SAG1), 0.31 (SAG2), and 0.44 (SAG3), respectively.

Article Snippet: Serum samples were collected from different laboratories around Shiraz and Tehran using commercial IgG and IgM ELISA kits (Euroimmun, Lubeck, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

Journal: Clinical and Experimental Immunology

Article Title: Splenectomy modulates the immune response but does not prevent joint inflammation in a mouse model of RA

doi: 10.1093/cei/uxac052

Figure Lengend Snippet: Comparison of the intracellular Ca 2+ signaling in B and T cells from inguinal and mesenteric LNs of the arthritic control ( n = 3) and -splenectomized ( n = 3) mice. Cells of the inguinal ( A , C , E ) and mesenteric ( B , D , F ) LNs were isolated and loaded with the Ca 2+ -specific indicator Fluo-3, then stimulated with selective activators for B or T cells. The intracellular Ca 2+ change was detected as time-dependent FL-1 fluorescence change for 5 min using a flow cytometer. The baseline was measured for 1 min, and then the cells were stimulated and measured for 5 min. B cells were activated using anti-IgM- ( A , B ) or anti-IgG ( C , D ) antibodies, while T cells ( E , F ), were activated using anti-CD3 antibody cross-linking. Values are presented as mean ± SEM.

Article Snippet: Levels of the sera rheumatoid factor (RF) IgG and IgM levels were measured using the FineTest Mouse RF-IgG or -IgM ELISA Kits (FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Comparison, Isolation, Fluorescence, Flow Cytometry

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Testing of Novel Dengue Virus 2 Vaccines in African Green Monkeys: Safety, Immunogenicity, and Efficacy

doi: 10.4269/ajtmh.2012.12-0004

Figure Lengend Snippet: Total IgM levels pre-challenge and post-challenge determined by enzyme-linked immunosorbent assay. Each bar indicates the average IgM optical density (OD) measured at 450 nm for four monkeys in each group, and error bars indicate SEM (n = 4). No statistical difference was seen between the amounts of IgM measured over the trial period of 142 days. LAV = live attenuated virus.

Article Snippet: Total IgG and IgM measurements were made by using Dengue IgG and IgM ELISA Kits (IBL-America, Minneapolis, MN).

Techniques: Enzyme-linked Immunosorbent Assay