Journal: The Journal of Experimental Medicine
Article Title: BAFF and MyD88 signals promote a lupuslike disease independent of T cells
Figure Lengend Snippet: Production of IgM and IgG, but not IgA, autoantibodies in TΔ-BTg mice. (A) ELISA was used to determine IgG, IgA, and IgM anti-ssDNA (top), anti-dsDNA (middle), and RF (bottom) in serum from 8-mo-old WT (circle), TCR −/− (diamond), BAFF Tg (square), and TΔ-BTg (triangle) mice. Symbols represent individual mice, and the mean for each group is indicated by columns. Significant P values are shown. n ≥ 6 per group. (B) Representative staining of isotype-specific ANA and anti-dsDNA were determined by staining Hep-2 and C. luciliae slides, respectively, with serum from indicated mice.
Article Snippet: Fluorescent-labeled anti–mouse antibodies CD1d, CD4, CD5, CD8, CD11c, CD21/CD35, CD25, CD44, CD45R/B220, CD62L, CD69, CD80, MHC class II, IgD, IgM, IgA, IgG subclasses (BD Biosciences) CD3, CD23, CD93 (eBioscience), BAFFR, TACI, BCMA (R & D Systems), and PDCA-1 (Miltenyi Biotec) were used for FACS and/or microscopy analysis.
Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Staining