igg subclass Search Results


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  • 99
    Thermo Fisher igg4 subclasses
    Positive and negative predictive value of the <t>IgG4-based</t> classifier
    Igg4 Subclasses, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore igg subclasses
    Dependence of inhibition on carbohydrate antigen structure. Measured <t>IgG</t> signals to Forssman disaccharide and tetrasaccharide (A) and four blood group A antigens (B) in the absence of added <t>IgM</t> (0 μg/mL) or in the presence of varying amounts of IgM (50–400 μg/mL).
    Igg Subclasses, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Merck & Co subclasses igg1 igg4
    Serological response to AAV-2 in Irish blood donors. (a) AAV-2-specific total <t>IgG</t> in human donor plasma ( n =45). Specific IgG was determined by indirect AAV-2 antigen ELISA, normalized by comparison with standard positive and negative samples. The negative cut-off (dashed line) was defined at 2 sd above the concentration of a known seronegative standard. (b–e) AAV-2-specific IgG subclasses present in IgG-positive samples ( n =41). <t>IgG1(b),</t> <t>IgG2</t> (c), <t>IgG3</t> (d) and <t>IgG4</t> (e) determined by AAV-2-specific direct antigen ELISA for triplicate samples and quantified by comparison with known reference sera.
    Subclasses Igg1 Igg4, supplied by Merck & Co, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SouthernBiotech igg4 igg subclasses
    <t>IgG</t> subclass titers for 012M and Fx017M cohorts. Antibody titers for IgG1, IgG2, IgG3, and <t>IgG4</t> for the 012M and Fx017M cohorts against three CSP test antigens: full-length recombinant CSP (left column), NANP repeat region peptide (middle column), and C-terminal PF16 peptide (right column). Protected and non-protected subjects are colored in blue and red, respectively.
    Igg4 Igg Subclasses, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Nordic-Mubio igg4
    Analysis of <t>IgG</t> responses to Ov-CHI-1 plotted against microfilarial skin densities for individual individuals from four endemic regions. Figure 8A : Nigeria, Figure 8B Ecuador, Figure 8C Togo, Figure 8D . Cameroon. The line of linear regression is shown on each graph.
    Igg4, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Dade Behring igg4 subclasses
    Recovery of HCV core protein after cold-precipitation in the presence of increasing amounts of <t>IgG</t> with specific anticore reactivity ( ) or irrelevant IgG (○). HCV core protein was premixed with purified IgM with RF activity.
    Igg4 Subclasses, supplied by Dade Behring, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad igg4 subclass ab
    Frequencies of IgG1 (A) and <t>IgG4</t> (B) positive individuals among FS patients and endemic controls (ECs) Bar graphs represent positive sera as defined by the cut points. FS patients (black bars) and ECs (white bars) are shown.
    Igg4 Subclass Ab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Athens Research igg4 subclasses monoclonal
    <t>IgG</t> binding characteristics of FcγR1
    Igg4 Subclasses Monoclonal, supplied by Athens Research, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Abcam igg subclass
    Associations of anti-ADAMTS13 <t>IgG1</t> and <t>IgG4</t> levels with the ADAMTS13 activity measured in 97 of the 100 deficient patient samples following mixing and incubation with equal amounts (1:1 ratio) of pooled normal human plasma or serum.
    Igg Subclass, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Athens Research igg3 subclasses
    <t>IgG</t> binding characteristics of FcγR1
    Igg3 Subclasses, supplied by Athens Research, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    SouthernBiotech igg subclasses
    Repeated immunizations retain T-cell immunogenicity in C57BL/6J and HBeAg-Tg mice . Groups of five to seven C57BL/6J and HBeAg-Tg mice were immunized once or twice with 5 μg coHBcAg plasmid DNA in combination with 5 μg IL-12 plasmid DNA by intramuscular immunization (IVIN-EP). Groups of mice were immunized twice with 4, 8, or 12 weeks between immunizations. Two weeks after last immunization, the mice were bled, sacrificed, and splenocytes harvested for determination of T-cell responses. In ( a ), the number of IFN-γ spot forming cells (SFCs) by enzyme-linked immunospot (ELISpot) assay in immunized C57BL/6J and HBeAg-Tg mice is shown. The ELISpot was determined after in vitro stimulation of splenocytes with peptides and recombinant proteins as indicated. Results are given as the mean SFCs/10 6 (+SD) splenocytes with a cutoff set at 50 SFCs/10 6 splenocytes. ( b ) Polyfunctionality (IFN-γ+, TNF-α+, CD107a+) of HBcAg-specific T-cell responses of splenocytes stimulated with overlapping peptide pools (0.3 μg/ml each peptide) covering the full-length HBcAg-protein. Each bar (black = C57BL/6J, white = HBeAg-Tg) represents the percentage of CD8 + T cells that are polyfunctional 12 hours after antigen stimulation. Gray bars represent C57BL/6J or HBeAg-Tg mice 2 weeks after a single immunization. ( c ) Serum from individual mice was evaluated for anti-HBc <t>IgG</t> antibody titers 2 weeks after last immunization. In graph each group, mean (+SD) endpoint dilution of anti-HBc IgG antibody is represented by black (C57BL/6J) or white (HBeAg-Tg) bars. Gray bars represent C57BL/6J or HBeAg-Tg mice 2 weeks after a single immunization. The statistical difference (* P
    Igg Subclasses, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson igg subclasses
    MyD88 signaling is essential for B cell activation and <t>IgG</t> autoantibody production in BAFF Tg mice. Lethally irradiated 6-wk-old BAFF Tg mice were reconstituted with MyD88 +/+ or MyD88 −/− or mixed Rag1 −/− MyD88 +/+ or Rag1 −/− MyD88 −/− BM, as indicated. (A) Histogram of B cell activation markers <t>CD44</t> (top) and CD69 (bottom) of Fo (left) and MZ (right) splenic B cells from MyD88 +/+ (solid line) and MyD88 −/− (dashed line) BAFF Tg BM chimeras. (B) Anti-dsDNA and RF antibody production in WT mice reconstituted with MyD88 +/+ (circles) or MyD88 −/− (diamonds); BAFF Tg mice reconstituted with MyD88 +/+ (squares; light gray bar); or MyD88 −/− (triangles; light gray bar); or BAFF Tg mice reconstituted with Rag1 −/− MyD88 +/+ (squares; dark gray bar); or Rag1 −/− MyD88 −/− (triangles; dark gray bar). IgG, IgA, and IgM (C) and C3 (D) deposition in the kidney of BAFF Tg mice reconstituted with MyD88 +/+ , MyD88 −/− , Rag1 −/− MyD88 +/+ , or Rag1 −/− MyD88 −/− BM, as indicated. Significant P values are shown.
    Igg Subclasses, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies igg subclass
    MyD88 signaling is essential for B cell activation and <t>IgG</t> autoantibody production in BAFF Tg mice. Lethally irradiated 6-wk-old BAFF Tg mice were reconstituted with MyD88 +/+ or MyD88 −/− or mixed Rag1 −/− MyD88 +/+ or Rag1 −/− MyD88 −/− BM, as indicated. (A) Histogram of B cell activation markers <t>CD44</t> (top) and CD69 (bottom) of Fo (left) and MZ (right) splenic B cells from MyD88 +/+ (solid line) and MyD88 −/− (dashed line) BAFF Tg BM chimeras. (B) Anti-dsDNA and RF antibody production in WT mice reconstituted with MyD88 +/+ (circles) or MyD88 −/− (diamonds); BAFF Tg mice reconstituted with MyD88 +/+ (squares; light gray bar); or MyD88 −/− (triangles; light gray bar); or BAFF Tg mice reconstituted with Rag1 −/− MyD88 +/+ (squares; dark gray bar); or Rag1 −/− MyD88 −/− (triangles; dark gray bar). IgG, IgA, and IgM (C) and C3 (D) deposition in the kidney of BAFF Tg mice reconstituted with MyD88 +/+ , MyD88 −/− , Rag1 −/− MyD88 +/+ , or Rag1 −/− MyD88 −/− BM, as indicated. Significant P values are shown.
    Igg Subclass, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore igg subclass
    <t>RBC</t> autoantibody responses 6 weeks after peptide inhalation. Comparison of total <t>IgG</t> ( a ), IgG1 ( b ) and IgG2a ( c ) responses of individual NZB mice 6 weeks after they had inhaled PBS or peptide 25 (241–251) or peptide 29 (282–296) or both (p25 + p29). Total IgG was significantly higher in p25 and p25 + p29 groups ( P
    Igg Subclass, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jackson Immuno igg subclasses
    The immunogenicity of Ig idiotypes (Ids) is profoundly influenced by the isotype, Fcγ piece, and state of <t>IgA</t> polymerization. The y axis shows the serum levels of <t>IgG</t> Ab against plates coated with the homologous Id borne by IgM, determined by ELISA as described in Materials and Methods . The immunogen and the priming and booster doses are indicated at the top left parts of A – H . Each symbol represents an individual mouse. Pre, preimmunization serum; Bld, below level of detection; mono, monomer; di/poly, dimer/polymer.
    Igg Subclasses, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad igg subclasses
    SjGP-3-specific immunoglobulin subtypes <t>(IgG1,</t> <t>IgG2a</t> and <t>IgE)</t> . Groups of mice were immunized with SjGP-3 formulations. Serum was collected after three time immunization. IgG1, IgG2a and IgE titers were detected by ELISA in triplicate wells respectively and the IgG2a to IgG1 ratio was calculated. (A) Anti-SjGP-3 antibody titers. (B) IgG2a/IgG1 ratio. * p
    Igg Subclasses, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Boehringer Mannheim igg1 subclass
    RIA quantitation of HBsAg levels in the supernatants of PLC/PRF/5 cells cultured with human monoclonal anti-HBs <t>IgG.</t> During the first period (open bars), the cells were cultured in the presence of medium with FCS and two concentrations of anti-HBs. In parallel, cells were cultured with medium plus FCS only, i.e., without human IgG (FCS) or with nonimmune human IgG (ABS), as controls. After 2 days of culture, the supernatants were collected, and the same cells were maintained in culture for a further two intervals of 2 days each (shaded and solid bars, respectively) in medium with FCS without human IgG. The HBsAg levels in the supernatants were tested at the end of each period, i.e., at three time points. The bars represent the means and standard deviations of two separate experiments, with each experimental condition run in duplicate.
    Igg1 Subclass, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Pharmingen igg2b subclasses
    IgM, IgG and IgG subclasses of alloantibodies to MHC class I antigens measured by flow cytometry in serum from C57BL/6 WT (square symbols) and FcγRIII-KO recipients (circle symbols) 7 days after transplantation FcγRIII-KO demonstrated higher levels of IgM (A) and IgG (B) alloantibodies to MHC class I antigens in the circulation than in WT recipients (titer > 64). IgG was mainly composed of C-activating IgG2a and <t>IgG2b</t> subclasses (C, D). The level of non-C activating IgG1 was very low in both FcγRIII-KO and WT recipients (E). Alloantibodies from FcγRIII-KO mice showed significantly higher ability to activate C and deposit C4d on target cells than their WT counterparts (F). Each point represents the average mode channel fluorescence staining +SD (n > 10). Statistically significant differences (p
    Igg2b Subclasses, supplied by Pharmingen, used in various techniques. Bioz Stars score: 84/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore subclass igg1
    IgM, IgG and IgG subclasses of alloantibodies to MHC class I antigens measured by flow cytometry in serum from C57BL/6 WT (square symbols) and FcγRIII-KO recipients (circle symbols) 7 days after transplantation FcγRIII-KO demonstrated higher levels of IgM (A) and IgG (B) alloantibodies to MHC class I antigens in the circulation than in WT recipients (titer > 64). IgG was mainly composed of C-activating IgG2a and <t>IgG2b</t> subclasses (C, D). The level of non-C activating IgG1 was very low in both FcγRIII-KO and WT recipients (E). Alloantibodies from FcγRIII-KO mice showed significantly higher ability to activate C and deposit C4d on target cells than their WT counterparts (F). Each point represents the average mode channel fluorescence staining +SD (n > 10). Statistically significant differences (p
    Subclass Igg1, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Nordic-Mubio mouse igg subclasses
    Induction of specific immune responses in IL-4Rα −/− mice receiving UreA-specific CD4 + T cells. (A) Proliferation of splenic CD4 + T cells isolated from recipient challenged or nonrecipient infected mice. Results are expressed as mean ± standard error of the mean. (B) Analysis of IgG2a and <t>IgG1</t> antibody responses in serum. Results are expressed as the mean ± standard deviation. IgG2a titers in recipient mice were significantly higher than in nonrecipient mice (∗) ( P
    Mouse Igg Subclasses, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Nordic-Mubio sheep anti human igg1
    Induction of specific immune responses in IL-4Rα −/− mice receiving UreA-specific CD4 + T cells. (A) Proliferation of splenic CD4 + T cells isolated from recipient challenged or nonrecipient infected mice. Results are expressed as mean ± standard error of the mean. (B) Analysis of IgG2a and <t>IgG1</t> antibody responses in serum. Results are expressed as the mean ± standard deviation. IgG2a titers in recipient mice were significantly higher than in nonrecipient mice (∗) ( P
    Sheep Anti Human Igg1, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Becton Dickinson subclass control igg
    Effects of G M1 and Aβ-(1–40) on <t>NSCs</t> ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control <t>IgG</t> (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).
    Subclass Control Igg, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    MedImmune igg1 subclass
    Effects of G M1 and Aβ-(1–40) on <t>NSCs</t> ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control <t>IgG</t> (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).
    Igg1 Subclass, supplied by MedImmune, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Agilent technologies nonimmune igg subclass
    Effects of G M1 and Aβ-(1–40) on <t>NSCs</t> ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control <t>IgG</t> (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).
    Nonimmune Igg Subclass, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Athens Research individual igg subclasses
    Effects of G M1 and Aβ-(1–40) on <t>NSCs</t> ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control <t>IgG</t> (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).
    Individual Igg Subclasses, supplied by Athens Research, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore myeloma igg subclasses
    Effects of G M1 and Aβ-(1–40) on <t>NSCs</t> ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control <t>IgG</t> (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).
    Myeloma Igg Subclasses, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    NeuroMab igg1 subclass
    Effects of G M1 and Aβ-(1–40) on <t>NSCs</t> ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control <t>IgG</t> (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).
    Igg1 Subclass, supplied by NeuroMab, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Biacore igg1 subclass
    Effects of G M1 and Aβ-(1–40) on <t>NSCs</t> ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control <t>IgG</t> (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).
    Igg1 Subclass, supplied by Biacore, used in various techniques. Bioz Stars score: 81/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Glycoform igg1 subclass
    Mean relative abundance of the percentage of G 0 F relative to the total glycoform abundance in the antibody subclasses among the groups of myositis patients, asymptomatic siblings, and unrelated age-matched controls. A) <t>IgG</t> 1 subclass and B) IgG 2–3
    Igg1 Subclass, supplied by Glycoform, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Jackson Immuno subclass nonspecific igg
    Depletion of CD301b + DCs leads to enhanced antibody responses to the antigen immunized with weak or no adjuvant. ( a ) Immunization and sample collection timeline. ( b ) Mgl2-DTR mice were treated with 0.5 µg DT or its inactive mutant CRM197 (CRM) on days -1 and +2 and immunized on day 0 with 50 µg papain plus indicated amount of OVA in the footpad. All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and sera were harvested on day 21 for OVA-specific antibody ELISA. ( c ) WT and Mgl2-DTR mice were treated with 0.5 µg DT on days −1 and +2 and immunized in the footpad with 5 µg OVA without any adjuvant on day 0 (top). Alternatively, lethally-irradiated WT mice were reconstituted with Mgl2-DTR (DTR only), WT (WT only) or 1:1-mixture of WT and Mgl2-DTR (WT+DTR) BM cells, then immunized with 5 µg OVA without adjuvant (bottom). All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and day 21, and sera were harvested on day 21 and day 28 for OVA-specific <t>IgG1</t> ELISA. Bars indicate mean ± S.E.M. calculated from 4–11 individual mice. n.s., not significant, *p
    Subclass Nonspecific Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Positive and negative predictive value of the IgG4-based classifier

    Journal:

    Article Title: Development of an IgG4-based Classifier/Predictor of Endemic Pemphigus Foliaceus (Fogo Selvagem)

    doi: 10.1038/jid.2008.189

    Figure Lengend Snippet: Positive and negative predictive value of the IgG4-based classifier

    Article Snippet: Following wash, the plate was incubated with a 1:2000 dilution (for IgG plates) 1:1000 dilution (for IgG subclasses) of a mouse horseradish peroxidase (HRP) labelled monoclonal antibody against human IgG or human IgG1, IgG2, IgG3 and IgG4 subclasses (Zymed, San Francisco, California) ( ; ).

    Techniques:

    Smooth density plots of index values of anti-Dsg1 IgG Subclasses and IgG in FS patients (red lines) and healthy donors (blue lines). Negative Index values were converted to zero before applying the log transformation.

    Journal:

    Article Title: Development of an IgG4-based Classifier/Predictor of Endemic Pemphigus Foliaceus (Fogo Selvagem)

    doi: 10.1038/jid.2008.189

    Figure Lengend Snippet: Smooth density plots of index values of anti-Dsg1 IgG Subclasses and IgG in FS patients (red lines) and healthy donors (blue lines). Negative Index values were converted to zero before applying the log transformation.

    Article Snippet: Following wash, the plate was incubated with a 1:2000 dilution (for IgG plates) 1:1000 dilution (for IgG subclasses) of a mouse horseradish peroxidase (HRP) labelled monoclonal antibody against human IgG or human IgG1, IgG2, IgG3 and IgG4 subclasses (Zymed, San Francisco, California) ( ; ).

    Techniques: Transformation Assay

    NC1-immunized SKH1 mice produce IgG autoantibodies which react specifically with skin basement membrane. Indirect immunofluorescence microscopy was used to examine the reactivity of autoreactive IgGs with the skin basement membrane zone component, as described in Materials and methods. Left and middle panels show IgG autoantibodies reacting with the skin basement membrane in mice immunized with NC1 in combinations of anti-CD25 or isotype control, respectively. The right panel shows the result of the serum from mouse treated with anti-CD25 and immunized with albumin. Images are representative for each group (original magnification ×40).

    Journal: Clinical and Experimental Immunology

    Article Title: Autoimmunity to type VII collagen in SKH1 mice is independent of regulatory T cells

    doi: 10.1111/j.1365-2249.2006.03115.x

    Figure Lengend Snippet: NC1-immunized SKH1 mice produce IgG autoantibodies which react specifically with skin basement membrane. Indirect immunofluorescence microscopy was used to examine the reactivity of autoreactive IgGs with the skin basement membrane zone component, as described in Materials and methods. Left and middle panels show IgG autoantibodies reacting with the skin basement membrane in mice immunized with NC1 in combinations of anti-CD25 or isotype control, respectively. The right panel shows the result of the serum from mouse treated with anti-CD25 and immunized with albumin. Images are representative for each group (original magnification ×40).

    Article Snippet: ELISA assays were also performed to characterize the subclasses and light chains of the IgG autoantibodies to NC1 as follows: for IgG subclass determination, a 96-well plate (Nunc-Immuno Plate, Nalge Nunc Int.) was coated with recombinant mouse NC1 and blocked as described above.

    Techniques: Mouse Assay, Immunofluorescence, Microscopy

    Dependence of inhibition on carbohydrate antigen structure. Measured IgG signals to Forssman disaccharide and tetrasaccharide (A) and four blood group A antigens (B) in the absence of added IgM (0 μg/mL) or in the presence of varying amounts of IgM (50–400 μg/mL).

    Journal: PLoS ONE

    Article Title: Competition between Serum IgG, IgM, and IgA Anti-Glycan Antibodies

    doi: 10.1371/journal.pone.0119298

    Figure Lengend Snippet: Dependence of inhibition on carbohydrate antigen structure. Measured IgG signals to Forssman disaccharide and tetrasaccharide (A) and four blood group A antigens (B) in the absence of added IgM (0 μg/mL) or in the presence of varying amounts of IgM (50–400 μg/mL).

    Article Snippet: Serum samples Purified serum antibodies (IgG I2511, IgM I8260, and IgA I4036) and all the different IgG subclasses (IgG1 I5154, IgG2 I5404, IgG3 I5654, IgG4 I4639) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Inhibition

    Dose-dependent inhibition of IgG and IgA signals upon addition of IgM. (A) Decrease in IgG signals with increase in IgM concentration. (B) Decrease in IgA signals with increase in IgM concentration. Polyclonal IgG and IgA isolated from serum were first profiled on the array alone. Separately, they were pre-mixed with varying amounts of IgM and then profiled on the array. The box plots depict the range of IgG or IgA signals (on a log base 2 scale) alone or in the presence of varying amounts of IgM (50–400 μg/mL). The line in the middle of the box is the median signal, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. The box plots demonstrate decreasing IgG and IgA signals in the presence of increasing amounts of IgM across the entire array.

    Journal: PLoS ONE

    Article Title: Competition between Serum IgG, IgM, and IgA Anti-Glycan Antibodies

    doi: 10.1371/journal.pone.0119298

    Figure Lengend Snippet: Dose-dependent inhibition of IgG and IgA signals upon addition of IgM. (A) Decrease in IgG signals with increase in IgM concentration. (B) Decrease in IgA signals with increase in IgM concentration. Polyclonal IgG and IgA isolated from serum were first profiled on the array alone. Separately, they were pre-mixed with varying amounts of IgM and then profiled on the array. The box plots depict the range of IgG or IgA signals (on a log base 2 scale) alone or in the presence of varying amounts of IgM (50–400 μg/mL). The line in the middle of the box is the median signal, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. The box plots demonstrate decreasing IgG and IgA signals in the presence of increasing amounts of IgM across the entire array.

    Article Snippet: Serum samples Purified serum antibodies (IgG I2511, IgM I8260, and IgA I4036) and all the different IgG subclasses (IgG1 I5154, IgG2 I5404, IgG3 I5654, IgG4 I4639) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Inhibition, Concentration Assay, Isolation, Standard Deviation

    Competition between serum IgG, IgA, and IgM anti-glycan antibodies. (A) Addition of IgM and IgA to IgG. Polyclonal IgG isolated from serum was first profiled on the array alone. Separately, IgG was premixed with varying amounts of IgM or IgA and then profiled on the array. For each array component, the change in IgG signal in the presence of IgM or IgA was determined. The box plots depict the range of IgG changes (on a log base 2 scale) measured on the array upon addition of 4 serum equivalents of IgM or IgA. The line in the middle of the box is the median, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. (B) Addition of IgG and IgA to IgM. An analogous protocol as above was used to evaluate effects of IgG and IgA on IgM signals. (C) Addition of IgM and IgG to IgA. An analogous protocol as above was used to evaluate effects of IgG and IgM on IgA signals. The box plots demonstrate significant decreases in IgG and IgA signals in the presence of IgM for the vast majority of array components.

    Journal: PLoS ONE

    Article Title: Competition between Serum IgG, IgM, and IgA Anti-Glycan Antibodies

    doi: 10.1371/journal.pone.0119298

    Figure Lengend Snippet: Competition between serum IgG, IgA, and IgM anti-glycan antibodies. (A) Addition of IgM and IgA to IgG. Polyclonal IgG isolated from serum was first profiled on the array alone. Separately, IgG was premixed with varying amounts of IgM or IgA and then profiled on the array. For each array component, the change in IgG signal in the presence of IgM or IgA was determined. The box plots depict the range of IgG changes (on a log base 2 scale) measured on the array upon addition of 4 serum equivalents of IgM or IgA. The line in the middle of the box is the median, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. (B) Addition of IgG and IgA to IgM. An analogous protocol as above was used to evaluate effects of IgG and IgA on IgM signals. (C) Addition of IgM and IgG to IgA. An analogous protocol as above was used to evaluate effects of IgG and IgM on IgA signals. The box plots demonstrate significant decreases in IgG and IgA signals in the presence of IgM for the vast majority of array components.

    Article Snippet: Serum samples Purified serum antibodies (IgG I2511, IgM I8260, and IgA I4036) and all the different IgG subclasses (IgG1 I5154, IgG2 I5404, IgG3 I5654, IgG4 I4639) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Isolation, Standard Deviation

    Changes in serum IgG and IgM signals in a pooled serum sample in the presence of varying amounts of added IgG, IgA, or IgM. (A) Changes in IgG signals upon addition of IgM. (B) Changes in IgG signals upon addition of IgA. (C) Changes in IgM signals upon addition of IgG. (D) Changes in IgM signals upon addition of IgA. IgG and IgM signals in a pooled serum sample were first profiled on the array alone. Separately, the serum sample was pre-mixed with varying amounts of IgG, IgA, or IgM and then profiled on the array. The box plots depict the range of IgG or IgM signals (on a log base 2 scale) alone or in the presence of varying amounts of IgG, IgA, or IgM. The line in the middle of the box is the median signal, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. The only significant decreases observed are for IgG signals upon addition of IgM.

    Journal: PLoS ONE

    Article Title: Competition between Serum IgG, IgM, and IgA Anti-Glycan Antibodies

    doi: 10.1371/journal.pone.0119298

    Figure Lengend Snippet: Changes in serum IgG and IgM signals in a pooled serum sample in the presence of varying amounts of added IgG, IgA, or IgM. (A) Changes in IgG signals upon addition of IgM. (B) Changes in IgG signals upon addition of IgA. (C) Changes in IgM signals upon addition of IgG. (D) Changes in IgM signals upon addition of IgA. IgG and IgM signals in a pooled serum sample were first profiled on the array alone. Separately, the serum sample was pre-mixed with varying amounts of IgG, IgA, or IgM and then profiled on the array. The box plots depict the range of IgG or IgM signals (on a log base 2 scale) alone or in the presence of varying amounts of IgG, IgA, or IgM. The line in the middle of the box is the median signal, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. The only significant decreases observed are for IgG signals upon addition of IgM.

    Article Snippet: Serum samples Purified serum antibodies (IgG I2511, IgM I8260, and IgA I4036) and all the different IgG subclasses (IgG1 I5154, IgG2 I5404, IgG3 I5654, IgG4 I4639) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Standard Deviation

    Serological response to AAV-2 in Irish blood donors. (a) AAV-2-specific total IgG in human donor plasma ( n =45). Specific IgG was determined by indirect AAV-2 antigen ELISA, normalized by comparison with standard positive and negative samples. The negative cut-off (dashed line) was defined at 2 sd above the concentration of a known seronegative standard. (b–e) AAV-2-specific IgG subclasses present in IgG-positive samples ( n =41). IgG1(b), IgG2 (c), IgG3 (d) and IgG4 (e) determined by AAV-2-specific direct antigen ELISA for triplicate samples and quantified by comparison with known reference sera.

    Journal: The Journal of General Virology

    Article Title: Adeno-associated virus serotype 2 induces cell-mediated immune responses directed against multiple epitopes of the capsid protein VP1

    doi: 10.1099/vir.0.014175-0

    Figure Lengend Snippet: Serological response to AAV-2 in Irish blood donors. (a) AAV-2-specific total IgG in human donor plasma ( n =45). Specific IgG was determined by indirect AAV-2 antigen ELISA, normalized by comparison with standard positive and negative samples. The negative cut-off (dashed line) was defined at 2 sd above the concentration of a known seronegative standard. (b–e) AAV-2-specific IgG subclasses present in IgG-positive samples ( n =41). IgG1(b), IgG2 (c), IgG3 (d) and IgG4 (e) determined by AAV-2-specific direct antigen ELISA for triplicate samples and quantified by comparison with known reference sera.

    Article Snippet: Plates were probed for human IgG using anti-human IgG subclass-biotin antibodies for subclasses IgG1–IgG4 (Merck).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

    IgG subclass titers for 012M and Fx017M cohorts. Antibody titers for IgG1, IgG2, IgG3, and IgG4 for the 012M and Fx017M cohorts against three CSP test antigens: full-length recombinant CSP (left column), NANP repeat region peptide (middle column), and C-terminal PF16 peptide (right column). Protected and non-protected subjects are colored in blue and red, respectively.

    Journal: Scientific Reports

    Article Title: Delayed fractional dose regimen of the RTS,S/AS01 malaria vaccine candidate enhances an IgG4 response that inhibits serum opsonophagocytosis

    doi: 10.1038/s41598-017-08526-5

    Figure Lengend Snippet: IgG subclass titers for 012M and Fx017M cohorts. Antibody titers for IgG1, IgG2, IgG3, and IgG4 for the 012M and Fx017M cohorts against three CSP test antigens: full-length recombinant CSP (left column), NANP repeat region peptide (middle column), and C-terminal PF16 peptide (right column). Protected and non-protected subjects are colored in blue and red, respectively.

    Article Snippet: Human IgG1, IgG2, IgG3, and IgG4 antibody isotype subclasses were detected by separately adding R-phycoerythrin (PE)-conjugated mouse anti-human IgG1, IgG2, IgG3, or IgG4 IgG subclasses (Southern Biotech, Inc., Birmingham, AL, USA).

    Techniques: Recombinant

    Serum opsonophagocytic activity following depletion of IgG isotype subclass antibodies. The change in peak OPA relative to the non-depleted sample, as measured by Mfreq, when the low-OPA and high-OPA pools from the 012M and Fx017M cohorts were depleted of IgG1, IgG3, IgG4, or IgG1 + IgG3.

    Journal: Scientific Reports

    Article Title: Delayed fractional dose regimen of the RTS,S/AS01 malaria vaccine candidate enhances an IgG4 response that inhibits serum opsonophagocytosis

    doi: 10.1038/s41598-017-08526-5

    Figure Lengend Snippet: Serum opsonophagocytic activity following depletion of IgG isotype subclass antibodies. The change in peak OPA relative to the non-depleted sample, as measured by Mfreq, when the low-OPA and high-OPA pools from the 012M and Fx017M cohorts were depleted of IgG1, IgG3, IgG4, or IgG1 + IgG3.

    Article Snippet: Human IgG1, IgG2, IgG3, and IgG4 antibody isotype subclasses were detected by separately adding R-phycoerythrin (PE)-conjugated mouse anti-human IgG1, IgG2, IgG3, or IgG4 IgG subclasses (Southern Biotech, Inc., Birmingham, AL, USA).

    Techniques: Activity Assay

    Serum opsonophagocytic activity following depletion of IgG isotype subclasses at a range of serum concentrations. The change in Mfreq relative to the non-depleted sample is shown following depletion of IgG1, IgG3, and IgG4 for the low-OPA and high-OPA pools for the 012M and Fx017M cohorts.

    Journal: Scientific Reports

    Article Title: Delayed fractional dose regimen of the RTS,S/AS01 malaria vaccine candidate enhances an IgG4 response that inhibits serum opsonophagocytosis

    doi: 10.1038/s41598-017-08526-5

    Figure Lengend Snippet: Serum opsonophagocytic activity following depletion of IgG isotype subclasses at a range of serum concentrations. The change in Mfreq relative to the non-depleted sample is shown following depletion of IgG1, IgG3, and IgG4 for the low-OPA and high-OPA pools for the 012M and Fx017M cohorts.

    Article Snippet: Human IgG1, IgG2, IgG3, and IgG4 antibody isotype subclasses were detected by separately adding R-phycoerythrin (PE)-conjugated mouse anti-human IgG1, IgG2, IgG3, or IgG4 IgG subclasses (Southern Biotech, Inc., Birmingham, AL, USA).

    Techniques: Activity Assay

    The antibody response induced after vaccination. BALB/c mice were immunised with one or two doses of whole virus vaccine (15 μg total protein). Groups of four mice were sacrificed after vaccination and the serum analysed by luminex bead-based assay and ELISA. (A) The IgG antibody response induced after vaccination. Beads coated with whole H7N1 virus were used to measure the kinetics of the IgG response in a bead based immunoassay. Data are presented as the mean fluorescent intensity (MFI) of each mouse and the line shows the mean MFI of the group of animals on each day after vaccination. The MFI increased significantly (*, P

    Journal: Influenza and Other Respiratory Viruses

    Article Title: A pilot study of the immune response to whole inactivated avian influenza H7N1 virus vaccine in mice

    doi: 10.1111/j.1750-2659.2009.00075.x

    Figure Lengend Snippet: The antibody response induced after vaccination. BALB/c mice were immunised with one or two doses of whole virus vaccine (15 μg total protein). Groups of four mice were sacrificed after vaccination and the serum analysed by luminex bead-based assay and ELISA. (A) The IgG antibody response induced after vaccination. Beads coated with whole H7N1 virus were used to measure the kinetics of the IgG response in a bead based immunoassay. Data are presented as the mean fluorescent intensity (MFI) of each mouse and the line shows the mean MFI of the group of animals on each day after vaccination. The MFI increased significantly (*, P

    Article Snippet: After incubation, the influenza specific ASC were detected using 2 μg/ml biotinylated class (IgG; 1030-08, IgA; 1040-08, IgM; 1020-08, Southern Biotechnology, Birmingham, AL, USA) and IgG subclass (IgG1; 1070-08, IgG2a; 1080-08, IgG2b; 1090-08, IgG3; 1100-08, Southern Biotechnology) specific antibodies and then extravidin peroxidase (Sigma, St. Louis, MO, USA).

    Techniques: Mouse Assay, Luminex, Bead-based Assay, Enzyme-linked Immunosorbent Assay

    Analysis of IgG responses to Ov-CHI-1 plotted against microfilarial skin densities for individual individuals from four endemic regions. Figure 8A : Nigeria, Figure 8B Ecuador, Figure 8C Togo, Figure 8D . Cameroon. The line of linear regression is shown on each graph.

    Journal: Filaria Journal

    Article Title: Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1.

    doi: 10.1186/1475-2883-2-6

    Figure Lengend Snippet: Analysis of IgG responses to Ov-CHI-1 plotted against microfilarial skin densities for individual individuals from four endemic regions. Figure 8A : Nigeria, Figure 8B Ecuador, Figure 8C Togo, Figure 8D . Cameroon. The line of linear regression is shown on each graph.

    Article Snippet: The plates then were probed with monoclonal antibodies to human IgG1 (1:200), IgG2 (1:100), IgG3 (1:200) and IgG4 (1:400) (Nordic: Set MAU/IgG1-4, Nordic Immunological Laboratories, Tilburg, The Netherlands) for 2 hours at room temperature, washed and probed again with goat anti-mouse antibody conjugated with horseradish peroxidase (Bio-Rad, Hemel Hempstead, UK) at the dilution 1:1000 for 1 hour at room temperature.

    Techniques:

    Analysis of IgG responses of human onchocerciasis to Ov-CHI-1 categorized by gender and comparison of responses in the sera of infected (INF) and putatively immune (PI) individuals from four endemic foci.

    Journal: Filaria Journal

    Article Title: Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1.

    doi: 10.1186/1475-2883-2-6

    Figure Lengend Snippet: Analysis of IgG responses of human onchocerciasis to Ov-CHI-1 categorized by gender and comparison of responses in the sera of infected (INF) and putatively immune (PI) individuals from four endemic foci.

    Article Snippet: The plates then were probed with monoclonal antibodies to human IgG1 (1:200), IgG2 (1:100), IgG3 (1:200) and IgG4 (1:400) (Nordic: Set MAU/IgG1-4, Nordic Immunological Laboratories, Tilburg, The Netherlands) for 2 hours at room temperature, washed and probed again with goat anti-mouse antibody conjugated with horseradish peroxidase (Bio-Rad, Hemel Hempstead, UK) at the dilution 1:1000 for 1 hour at room temperature.

    Techniques: Infection

    Analysis of IgG responses to Ov-CHI-1 with respect to skin nodule number for individual subject from four endemic regions. Figure 9A : Cameroon, Figure 9B : Ecuador, Figure 9C : Nigeria.

    Journal: Filaria Journal

    Article Title: Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1.

    doi: 10.1186/1475-2883-2-6

    Figure Lengend Snippet: Analysis of IgG responses to Ov-CHI-1 with respect to skin nodule number for individual subject from four endemic regions. Figure 9A : Cameroon, Figure 9B : Ecuador, Figure 9C : Nigeria.

    Article Snippet: The plates then were probed with monoclonal antibodies to human IgG1 (1:200), IgG2 (1:100), IgG3 (1:200) and IgG4 (1:400) (Nordic: Set MAU/IgG1-4, Nordic Immunological Laboratories, Tilburg, The Netherlands) for 2 hours at room temperature, washed and probed again with goat anti-mouse antibody conjugated with horseradish peroxidase (Bio-Rad, Hemel Hempstead, UK) at the dilution 1:1000 for 1 hour at room temperature.

    Techniques:

    IgG subclass analysis by ELISA on Ov-CHI-1 full-length antigen using 77 selected sera (see text). The whole profile of IgG1-IgG4 responses is shown in Figure 2A . Figure 2B shows the relationship between microfilarial skin density and anti-Ov-CHI-1 IgG3 level in these sera. The relationship of IgG1 with IgG3 is shown in Figure 2C .

    Journal: Filaria Journal

    Article Title: Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1.

    doi: 10.1186/1475-2883-2-6

    Figure Lengend Snippet: IgG subclass analysis by ELISA on Ov-CHI-1 full-length antigen using 77 selected sera (see text). The whole profile of IgG1-IgG4 responses is shown in Figure 2A . Figure 2B shows the relationship between microfilarial skin density and anti-Ov-CHI-1 IgG3 level in these sera. The relationship of IgG1 with IgG3 is shown in Figure 2C .

    Article Snippet: The plates then were probed with monoclonal antibodies to human IgG1 (1:200), IgG2 (1:100), IgG3 (1:200) and IgG4 (1:400) (Nordic: Set MAU/IgG1-4, Nordic Immunological Laboratories, Tilburg, The Netherlands) for 2 hours at room temperature, washed and probed again with goat anti-mouse antibody conjugated with horseradish peroxidase (Bio-Rad, Hemel Hempstead, UK) at the dilution 1:1000 for 1 hour at room temperature.

    Techniques: Enzyme-linked Immunosorbent Assay

    ELISA analysis of the IgG response to recombinant Ov-CHI-1 5' or 3' antigen in human onchocerciasis. Figure 4A : Comparison of the response in the sera of infected (INF) and putatively immune (PI) individuals. Figure 4B : The relationship of response to 5' antigen and 3' antigen for individual sera. The line of liner regression is shown for data sets in which there is a statistically significant correlation between responses to the two antigens (determined by Spearman's Rank Correlation Coefficient).

    Journal: Filaria Journal

    Article Title: Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1.

    doi: 10.1186/1475-2883-2-6

    Figure Lengend Snippet: ELISA analysis of the IgG response to recombinant Ov-CHI-1 5' or 3' antigen in human onchocerciasis. Figure 4A : Comparison of the response in the sera of infected (INF) and putatively immune (PI) individuals. Figure 4B : The relationship of response to 5' antigen and 3' antigen for individual sera. The line of liner regression is shown for data sets in which there is a statistically significant correlation between responses to the two antigens (determined by Spearman's Rank Correlation Coefficient).

    Article Snippet: The plates then were probed with monoclonal antibodies to human IgG1 (1:200), IgG2 (1:100), IgG3 (1:200) and IgG4 (1:400) (Nordic: Set MAU/IgG1-4, Nordic Immunological Laboratories, Tilburg, The Netherlands) for 2 hours at room temperature, washed and probed again with goat anti-mouse antibody conjugated with horseradish peroxidase (Bio-Rad, Hemel Hempstead, UK) at the dilution 1:1000 for 1 hour at room temperature.

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Infection

    Analysis of IgG responses to Ov-CHI-1 categorized by age for individual individuals from four endemic regions. Figure 6A : Nigeria, Figure 6B : Ecuador, Figure 6C : Togo, Figure 6D : Cameroon.

    Journal: Filaria Journal

    Article Title: Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1.

    doi: 10.1186/1475-2883-2-6

    Figure Lengend Snippet: Analysis of IgG responses to Ov-CHI-1 categorized by age for individual individuals from four endemic regions. Figure 6A : Nigeria, Figure 6B : Ecuador, Figure 6C : Togo, Figure 6D : Cameroon.

    Article Snippet: The plates then were probed with monoclonal antibodies to human IgG1 (1:200), IgG2 (1:100), IgG3 (1:200) and IgG4 (1:400) (Nordic: Set MAU/IgG1-4, Nordic Immunological Laboratories, Tilburg, The Netherlands) for 2 hours at room temperature, washed and probed again with goat anti-mouse antibody conjugated with horseradish peroxidase (Bio-Rad, Hemel Hempstead, UK) at the dilution 1:1000 for 1 hour at room temperature.

    Techniques:

    ELISA analysis of the IgG response to recombinant Ov-CHI-1 in human onchocerciasis. Figure 1A , Comparison of the response in sera of endemic individuals from 4 endemic foci with uninfected controls from the United Kingdom. Mean values are indicated by a horizontal line. Figure 1B , Comparison of the response in the sera of infected (INF) and putatively immune (PI) individuals from 4 endemic foci and from uninfected UK controls. Box and whisker plots illustrate the group mean values (horizontal bar), data points falling within the 25th to 75th percentile (boxed) and the range (vertical lines). * P =

    Journal: Filaria Journal

    Article Title: Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1.

    doi: 10.1186/1475-2883-2-6

    Figure Lengend Snippet: ELISA analysis of the IgG response to recombinant Ov-CHI-1 in human onchocerciasis. Figure 1A , Comparison of the response in sera of endemic individuals from 4 endemic foci with uninfected controls from the United Kingdom. Mean values are indicated by a horizontal line. Figure 1B , Comparison of the response in the sera of infected (INF) and putatively immune (PI) individuals from 4 endemic foci and from uninfected UK controls. Box and whisker plots illustrate the group mean values (horizontal bar), data points falling within the 25th to 75th percentile (boxed) and the range (vertical lines). * P =

    Article Snippet: The plates then were probed with monoclonal antibodies to human IgG1 (1:200), IgG2 (1:100), IgG3 (1:200) and IgG4 (1:400) (Nordic: Set MAU/IgG1-4, Nordic Immunological Laboratories, Tilburg, The Netherlands) for 2 hours at room temperature, washed and probed again with goat anti-mouse antibody conjugated with horseradish peroxidase (Bio-Rad, Hemel Hempstead, UK) at the dilution 1:1000 for 1 hour at room temperature.

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Infection, Whisker Assay

    The development of IgG subclass responses to RSV measured by ELISA on pooled serum samples following two subcutaneous (s.c.) or two intranasal (i.n.) immunizations 6 weeks apart with RSV ISCOMs or two s.c. immunizations with inactivated virus, and non-immunized control. (a) First bleed, 2 weeks after the first immunization. (b) Second bleed, 5 weeks after the first immunization. (c) Third bleed, 1 week after the second immunization. (d) Fourth bleed, 3 weeks after the second immunization.

    Journal: Clinical and Experimental Immunology

    Article Title: The immunostimulating complex (ISCOM) is an efficient mucosal delivery system for respiratory syncytial virus (RSV) envelope antigens inducing high local and systemic antibody responses

    doi: 10.1046/j.1365-2249.1998.00650.x

    Figure Lengend Snippet: The development of IgG subclass responses to RSV measured by ELISA on pooled serum samples following two subcutaneous (s.c.) or two intranasal (i.n.) immunizations 6 weeks apart with RSV ISCOMs or two s.c. immunizations with inactivated virus, and non-immunized control. (a) First bleed, 2 weeks after the first immunization. (b) Second bleed, 5 weeks after the first immunization. (c) Third bleed, 1 week after the second immunization. (d) Fourth bleed, 3 weeks after the second immunization.

    Article Snippet: ELISA employed to determine the antibody responses in the IgG1, IgG2a, IgG2b, IgG3 subclasses, and the IgE and IgA isotypes were carried out with non-labelled goat antibodies specific for mouse IgG subclasses (Nordic Immunology, Tilburg, The Netherlands), biotinylated goat anti-mouse IgE (The Binding Site, Birmingham, UK) or biotinylated goat anti-mouse IgA (Southern Biotechnology Associates, Birmingham, AL), and a rabbit anti-goat horseradish peroxidase (HRP) conjugate or an HRP streptavidin (Dakopatts, Glostrup, Denmark), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay

    IgG antibody responses (geometric mean titres and 95% confidence limits) to RSV in upper respiratory tract (URT) and lung secretions, 2 weeks after the second immunization, as measured by ELISA, following two subcutaneous (s.c.) or two intranasal (i.n.) immunizations 6 weeks apart with RSV ISCOMs, and non-immunized control.

    Journal: Clinical and Experimental Immunology

    Article Title: The immunostimulating complex (ISCOM) is an efficient mucosal delivery system for respiratory syncytial virus (RSV) envelope antigens inducing high local and systemic antibody responses

    doi: 10.1046/j.1365-2249.1998.00650.x

    Figure Lengend Snippet: IgG antibody responses (geometric mean titres and 95% confidence limits) to RSV in upper respiratory tract (URT) and lung secretions, 2 weeks after the second immunization, as measured by ELISA, following two subcutaneous (s.c.) or two intranasal (i.n.) immunizations 6 weeks apart with RSV ISCOMs, and non-immunized control.

    Article Snippet: ELISA employed to determine the antibody responses in the IgG1, IgG2a, IgG2b, IgG3 subclasses, and the IgE and IgA isotypes were carried out with non-labelled goat antibodies specific for mouse IgG subclasses (Nordic Immunology, Tilburg, The Netherlands), biotinylated goat anti-mouse IgE (The Binding Site, Birmingham, UK) or biotinylated goat anti-mouse IgA (Southern Biotechnology Associates, Birmingham, AL), and a rabbit anti-goat horseradish peroxidase (HRP) conjugate or an HRP streptavidin (Dakopatts, Glostrup, Denmark), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay

    The development of IgG serum antibody titres (geometric mean titres and 95% confidence limits) of mice to RSV measured by ELISA following two subcutaneous (s.c.) or two intranasal (i.n.) immunizations with RSV ISCOMs or two s.c. immunizations with inactivated virus, and non-immunized control.

    Journal: Clinical and Experimental Immunology

    Article Title: The immunostimulating complex (ISCOM) is an efficient mucosal delivery system for respiratory syncytial virus (RSV) envelope antigens inducing high local and systemic antibody responses

    doi: 10.1046/j.1365-2249.1998.00650.x

    Figure Lengend Snippet: The development of IgG serum antibody titres (geometric mean titres and 95% confidence limits) of mice to RSV measured by ELISA following two subcutaneous (s.c.) or two intranasal (i.n.) immunizations with RSV ISCOMs or two s.c. immunizations with inactivated virus, and non-immunized control.

    Article Snippet: ELISA employed to determine the antibody responses in the IgG1, IgG2a, IgG2b, IgG3 subclasses, and the IgE and IgA isotypes were carried out with non-labelled goat antibodies specific for mouse IgG subclasses (Nordic Immunology, Tilburg, The Netherlands), biotinylated goat anti-mouse IgE (The Binding Site, Birmingham, UK) or biotinylated goat anti-mouse IgA (Southern Biotechnology Associates, Birmingham, AL), and a rabbit anti-goat horseradish peroxidase (HRP) conjugate or an HRP streptavidin (Dakopatts, Glostrup, Denmark), respectively.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Recovery of HCV core protein after cold-precipitation in the presence of increasing amounts of IgG with specific anticore reactivity ( ) or irrelevant IgG (○). HCV core protein was premixed with purified IgM with RF activity.

    Journal: Clinical and Experimental Immunology

    Article Title: Non-enveloped HCV core protein as constitutive antigen of cold-precipitable immune complexes in type II mixed cryoglobulinaemia

    doi: 10.1046/j.1365-2249.2003.02204.x

    Figure Lengend Snippet: Recovery of HCV core protein after cold-precipitation in the presence of increasing amounts of IgG with specific anticore reactivity ( ) or irrelevant IgG (○). HCV core protein was premixed with purified IgM with RF activity.

    Article Snippet: Concentrations of IgM, IgG, C3 and C4 fractions, RF activity (Olympus Diagnostics GmbH, Hamburg, Germany), IgG1, IgG2, IgG3 and IgG4 subclasses (NAS IgG1-4, Dade Behring), C1q and C1q binding activity (N Latex CIC, Dade Behring) were measured by nephelometry.

    Techniques: Purification, Activity Assay

    Frequencies of IgG1 (A) and IgG4 (B) positive individuals among FS patients and endemic controls (ECs) Bar graphs represent positive sera as defined by the cut points. FS patients (black bars) and ECs (white bars) are shown.

    Journal: ImmunoHorizons

    Article Title: Divergent Specificity Development of IgG1 and IgG4 Autoantibodies in Endemic Pemphigus Foliaceus (Fogo Selvagem)

    doi: 10.4049/immunohorizons.1700029

    Figure Lengend Snippet: Frequencies of IgG1 (A) and IgG4 (B) positive individuals among FS patients and endemic controls (ECs) Bar graphs represent positive sera as defined by the cut points. FS patients (black bars) and ECs (white bars) are shown.

    Article Snippet: After washes, separate plates were incubated with diluted HRP-conjugated goat anti-human IgG1 or IgG4 subclass Ab (Bio-Rad, Hercules, CA) for 1 h (1:1000 for seven desmosomal Ags and 1: 1500 for E-cad).

    Techniques:

    IgG1 (upper panels) and IgG4 (lower panels) anti-keratinocyte cadherin responses differ among FS patients, endemic controls, and United States controls (US) Sera from FS patients, endemic controls (EC), and United States controls were tested for IgG1 and IgG4 reactivity to Dsg1, Dsg2, Dsg3, Dsg4, Dsc1, Dsc2, Dsc3, and E-cad by ELISA. Index values were calculated, and the results for each population are displayed as box plots. To use a logarithmic scale, we shifted index values in each plot by an amount specific to that plot to make the lowest value equal 1. Each box extends from the lower to the upper quartile (25–75%), with the median shown within the box. A significant difference between paired groups is marked by an asterisk (*).

    Journal: ImmunoHorizons

    Article Title: Divergent Specificity Development of IgG1 and IgG4 Autoantibodies in Endemic Pemphigus Foliaceus (Fogo Selvagem)

    doi: 10.4049/immunohorizons.1700029

    Figure Lengend Snippet: IgG1 (upper panels) and IgG4 (lower panels) anti-keratinocyte cadherin responses differ among FS patients, endemic controls, and United States controls (US) Sera from FS patients, endemic controls (EC), and United States controls were tested for IgG1 and IgG4 reactivity to Dsg1, Dsg2, Dsg3, Dsg4, Dsc1, Dsc2, Dsc3, and E-cad by ELISA. Index values were calculated, and the results for each population are displayed as box plots. To use a logarithmic scale, we shifted index values in each plot by an amount specific to that plot to make the lowest value equal 1. Each box extends from the lower to the upper quartile (25–75%), with the median shown within the box. A significant difference between paired groups is marked by an asterisk (*).

    Article Snippet: After washes, separate plates were incubated with diluted HRP-conjugated goat anti-human IgG1 or IgG4 subclass Ab (Bio-Rad, Hercules, CA) for 1 h (1:1000 for seven desmosomal Ags and 1: 1500 for E-cad).

    Techniques: Enzyme-linked Immunosorbent Assay

    Serum IgG1 and IgG4 Ab levels against native Dsg1 in comparison with those against denatured Dsg1 The IgG1 and IgG4 Abs sera from these 11 individuals before (pre) and after (post) their onset of FS against were tested against native and denatured Dsg1. The ratios of Ab levels against native Dsg1 to those against denatured Dsg1 were calculated (anti-native Dsg1 OD/anti-denatured Dsg1 OD) and plotted. The p values were determined using Wilcoxon rank sum test.

    Journal: ImmunoHorizons

    Article Title: Divergent Specificity Development of IgG1 and IgG4 Autoantibodies in Endemic Pemphigus Foliaceus (Fogo Selvagem)

    doi: 10.4049/immunohorizons.1700029

    Figure Lengend Snippet: Serum IgG1 and IgG4 Ab levels against native Dsg1 in comparison with those against denatured Dsg1 The IgG1 and IgG4 Abs sera from these 11 individuals before (pre) and after (post) their onset of FS against were tested against native and denatured Dsg1. The ratios of Ab levels against native Dsg1 to those against denatured Dsg1 were calculated (anti-native Dsg1 OD/anti-denatured Dsg1 OD) and plotted. The p values were determined using Wilcoxon rank sum test.

    Article Snippet: After washes, separate plates were incubated with diluted HRP-conjugated goat anti-human IgG1 or IgG4 subclass Ab (Bio-Rad, Hercules, CA) for 1 h (1:1000 for seven desmosomal Ags and 1: 1500 for E-cad).

    Techniques:

    IgG binding characteristics of FcγR1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: IgG binding characteristics of FcγR1

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: Binding Assay

    Summary of affinities of rhesus macaque IgG samples across MM FcγR as determined by SPR

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: Summary of affinities of rhesus macaque IgG samples across MM FcγR as determined by SPR

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: SPR Assay

    IgG binding characteristics of low affinity MM FcγR

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: IgG binding characteristics of low affinity MM FcγR

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: Binding Assay

    Associations of anti-ADAMTS13 IgG1 and IgG4 levels with the ADAMTS13 activity measured in 97 of the 100 deficient patient samples following mixing and incubation with equal amounts (1:1 ratio) of pooled normal human plasma or serum.

    Journal: Frontiers in Immunology

    Article Title: Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura

    doi: 10.3389/fimmu.2018.01646

    Figure Lengend Snippet: Associations of anti-ADAMTS13 IgG1 and IgG4 levels with the ADAMTS13 activity measured in 97 of the 100 deficient patient samples following mixing and incubation with equal amounts (1:1 ratio) of pooled normal human plasma or serum.

    Article Snippet: Briefly, we prepared calibrator plates by coating two columns of 96-well polystyrene microtiter plates (Greiner Bio One International GmbH, Kremsmünster, Austria) with known concentrations of purified human antibodies of each IgG subclass (Abcam, Cambridge, UK; Ref. No: IgG1—ab90283, IgG2—ab90284, IgG3—ab118426, IgG4—ab90286).

    Techniques: Activity Assay, Incubation

    Anti-ADAMTS13 IgG levels in patients carrying or not carrying protective (DR7-DQ2 or DR13-DQ6) or risk (DR11-DQ3 or DR15-DQ6) HLA-DR-DQ haplotypes. Medians and interquartile ranges are shown. (A,B) Anti-ADAMTS13 IgG levels in the first available acute sample (either from the first acute episode or from relapse) of each patient with available HLA-DR-DQ data. The numbers below panel (B) show the number of risk or protective haplotypes carried by the patient. (C) Samples from the first acute episode and from relapse. +: protective haplotype carrier, −: not carrying protective haplotypes.

    Journal: Frontiers in Immunology

    Article Title: Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura

    doi: 10.3389/fimmu.2018.01646

    Figure Lengend Snippet: Anti-ADAMTS13 IgG levels in patients carrying or not carrying protective (DR7-DQ2 or DR13-DQ6) or risk (DR11-DQ3 or DR15-DQ6) HLA-DR-DQ haplotypes. Medians and interquartile ranges are shown. (A,B) Anti-ADAMTS13 IgG levels in the first available acute sample (either from the first acute episode or from relapse) of each patient with available HLA-DR-DQ data. The numbers below panel (B) show the number of risk or protective haplotypes carried by the patient. (C) Samples from the first acute episode and from relapse. +: protective haplotype carrier, −: not carrying protective haplotypes.

    Article Snippet: Briefly, we prepared calibrator plates by coating two columns of 96-well polystyrene microtiter plates (Greiner Bio One International GmbH, Kremsmünster, Austria) with known concentrations of purified human antibodies of each IgG subclass (Abcam, Cambridge, UK; Ref. No: IgG1—ab90283, IgG2—ab90284, IgG3—ab118426, IgG4—ab90286).

    Techniques:

    Number of patients and samples tested for anti-ADAMTS13 IgG subclass distribution according to disease stage. The number of common patients between disease stage groups are shown in the small boxes over the connecting lines.

    Journal: Frontiers in Immunology

    Article Title: Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura

    doi: 10.3389/fimmu.2018.01646

    Figure Lengend Snippet: Number of patients and samples tested for anti-ADAMTS13 IgG subclass distribution according to disease stage. The number of common patients between disease stage groups are shown in the small boxes over the connecting lines.

    Article Snippet: Briefly, we prepared calibrator plates by coating two columns of 96-well polystyrene microtiter plates (Greiner Bio One International GmbH, Kremsmünster, Austria) with known concentrations of purified human antibodies of each IgG subclass (Abcam, Cambridge, UK; Ref. No: IgG1—ab90283, IgG2—ab90284, IgG3—ab118426, IgG4—ab90286).

    Techniques:

    Concentration and subclass distribution of anti-ADAMTS13 IgG autoantibodies in the first acute phase, in relapse, and in remission. The horizontal line marks the cutoff value (15 U/mL).

    Journal: Frontiers in Immunology

    Article Title: Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura

    doi: 10.3389/fimmu.2018.01646

    Figure Lengend Snippet: Concentration and subclass distribution of anti-ADAMTS13 IgG autoantibodies in the first acute phase, in relapse, and in remission. The horizontal line marks the cutoff value (15 U/mL).

    Article Snippet: Briefly, we prepared calibrator plates by coating two columns of 96-well polystyrene microtiter plates (Greiner Bio One International GmbH, Kremsmünster, Austria) with known concentrations of purified human antibodies of each IgG subclass (Abcam, Cambridge, UK; Ref. No: IgG1—ab90283, IgG2—ab90284, IgG3—ab118426, IgG4—ab90286).

    Techniques: Concentration Assay

    Dominant subclasses in samples of patients with multiple samples. Dashed arrows show a change in the dominant subclass, arrows indicating a change from IgG1 to IgG4 appear in bold. The only sample with normal ADAMTS13 activity (with low positive levels of anti-ADAMTS13 antibodies) is marked by an asterisk.

    Journal: Frontiers in Immunology

    Article Title: Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura

    doi: 10.3389/fimmu.2018.01646

    Figure Lengend Snippet: Dominant subclasses in samples of patients with multiple samples. Dashed arrows show a change in the dominant subclass, arrows indicating a change from IgG1 to IgG4 appear in bold. The only sample with normal ADAMTS13 activity (with low positive levels of anti-ADAMTS13 antibodies) is marked by an asterisk.

    Article Snippet: Briefly, we prepared calibrator plates by coating two columns of 96-well polystyrene microtiter plates (Greiner Bio One International GmbH, Kremsmünster, Austria) with known concentrations of purified human antibodies of each IgG subclass (Abcam, Cambridge, UK; Ref. No: IgG1—ab90283, IgG2—ab90284, IgG3—ab118426, IgG4—ab90286).

    Techniques: Activity Assay

    Specific inhibitory potentials of samples from different disease stages and with different IgG4 proportions. Patient samples were diluted in order to have equal (25 U/mL) anti-ADAMTS13 concentrations, diluted samples were mixed and incubated in different ratios with normal pooled human citrated plasma or serum, and ADAMTS13 activities of the mixed samples were measured. Low activities of mixed samples indicate a strong inhibitory potential and vice versa . (A) Specific inhibitory potentials of acute samples. Mean and SEM are shown for all mixing ratios. All deficient samples from the relapse phase were IgG4 dominant; none had IgG4 proportions below 30%. (B) Specific inhibitory potentials of all samples stratified by dominant IgG subclass. (C) Specific inhibitory potentials of IgG4 dominant samples stratified by disease stage. All deficient samples taken during or following a relapse were IgG4 dominant.

    Journal: Frontiers in Immunology

    Article Title: Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura

    doi: 10.3389/fimmu.2018.01646

    Figure Lengend Snippet: Specific inhibitory potentials of samples from different disease stages and with different IgG4 proportions. Patient samples were diluted in order to have equal (25 U/mL) anti-ADAMTS13 concentrations, diluted samples were mixed and incubated in different ratios with normal pooled human citrated plasma or serum, and ADAMTS13 activities of the mixed samples were measured. Low activities of mixed samples indicate a strong inhibitory potential and vice versa . (A) Specific inhibitory potentials of acute samples. Mean and SEM are shown for all mixing ratios. All deficient samples from the relapse phase were IgG4 dominant; none had IgG4 proportions below 30%. (B) Specific inhibitory potentials of all samples stratified by dominant IgG subclass. (C) Specific inhibitory potentials of IgG4 dominant samples stratified by disease stage. All deficient samples taken during or following a relapse were IgG4 dominant.

    Article Snippet: Briefly, we prepared calibrator plates by coating two columns of 96-well polystyrene microtiter plates (Greiner Bio One International GmbH, Kremsmünster, Austria) with known concentrations of purified human antibodies of each IgG subclass (Abcam, Cambridge, UK; Ref. No: IgG1—ab90283, IgG2—ab90284, IgG3—ab118426, IgG4—ab90286).

    Techniques: Incubation

    IgG binding characteristics of FcγR1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: IgG binding characteristics of FcγR1

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: Binding Assay

    Summary of affinities of rhesus macaque IgG samples across MM FcγR as determined by SPR

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: Summary of affinities of rhesus macaque IgG samples across MM FcγR as determined by SPR

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: SPR Assay

    IgG binding characteristics of low affinity MM FcγR

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: IgG binding characteristics of low affinity MM FcγR

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: Binding Assay

    Repeated immunizations retain T-cell immunogenicity in C57BL/6J and HBeAg-Tg mice . Groups of five to seven C57BL/6J and HBeAg-Tg mice were immunized once or twice with 5 μg coHBcAg plasmid DNA in combination with 5 μg IL-12 plasmid DNA by intramuscular immunization (IVIN-EP). Groups of mice were immunized twice with 4, 8, or 12 weeks between immunizations. Two weeks after last immunization, the mice were bled, sacrificed, and splenocytes harvested for determination of T-cell responses. In ( a ), the number of IFN-γ spot forming cells (SFCs) by enzyme-linked immunospot (ELISpot) assay in immunized C57BL/6J and HBeAg-Tg mice is shown. The ELISpot was determined after in vitro stimulation of splenocytes with peptides and recombinant proteins as indicated. Results are given as the mean SFCs/10 6 (+SD) splenocytes with a cutoff set at 50 SFCs/10 6 splenocytes. ( b ) Polyfunctionality (IFN-γ+, TNF-α+, CD107a+) of HBcAg-specific T-cell responses of splenocytes stimulated with overlapping peptide pools (0.3 μg/ml each peptide) covering the full-length HBcAg-protein. Each bar (black = C57BL/6J, white = HBeAg-Tg) represents the percentage of CD8 + T cells that are polyfunctional 12 hours after antigen stimulation. Gray bars represent C57BL/6J or HBeAg-Tg mice 2 weeks after a single immunization. ( c ) Serum from individual mice was evaluated for anti-HBc IgG antibody titers 2 weeks after last immunization. In graph each group, mean (+SD) endpoint dilution of anti-HBc IgG antibody is represented by black (C57BL/6J) or white (HBeAg-Tg) bars. Gray bars represent C57BL/6J or HBeAg-Tg mice 2 weeks after a single immunization. The statistical difference (* P

    Journal: Molecular Therapy

    Article Title: Functional Aspects of Intrahepatic Hepatitis B Virus-specific T Cells Induced by Therapeutic DNA Vaccination

    doi: 10.1038/mt.2014.233

    Figure Lengend Snippet: Repeated immunizations retain T-cell immunogenicity in C57BL/6J and HBeAg-Tg mice . Groups of five to seven C57BL/6J and HBeAg-Tg mice were immunized once or twice with 5 μg coHBcAg plasmid DNA in combination with 5 μg IL-12 plasmid DNA by intramuscular immunization (IVIN-EP). Groups of mice were immunized twice with 4, 8, or 12 weeks between immunizations. Two weeks after last immunization, the mice were bled, sacrificed, and splenocytes harvested for determination of T-cell responses. In ( a ), the number of IFN-γ spot forming cells (SFCs) by enzyme-linked immunospot (ELISpot) assay in immunized C57BL/6J and HBeAg-Tg mice is shown. The ELISpot was determined after in vitro stimulation of splenocytes with peptides and recombinant proteins as indicated. Results are given as the mean SFCs/10 6 (+SD) splenocytes with a cutoff set at 50 SFCs/10 6 splenocytes. ( b ) Polyfunctionality (IFN-γ+, TNF-α+, CD107a+) of HBcAg-specific T-cell responses of splenocytes stimulated with overlapping peptide pools (0.3 μg/ml each peptide) covering the full-length HBcAg-protein. Each bar (black = C57BL/6J, white = HBeAg-Tg) represents the percentage of CD8 + T cells that are polyfunctional 12 hours after antigen stimulation. Gray bars represent C57BL/6J or HBeAg-Tg mice 2 weeks after a single immunization. ( c ) Serum from individual mice was evaluated for anti-HBc IgG antibody titers 2 weeks after last immunization. In graph each group, mean (+SD) endpoint dilution of anti-HBc IgG antibody is represented by black (C57BL/6J) or white (HBeAg-Tg) bars. Gray bars represent C57BL/6J or HBeAg-Tg mice 2 weeks after a single immunization. The statistical difference (* P

    Article Snippet: Detection of IgG subclasses (IgG1, IgG2a, IgG2b, and IgG3) (Southern Biotech, Birmingham, AL) was performed in a similar manner as described above with the exception that serum antibodies were detected using subclass-specific anti-mouse horseradish peroxidase–conjugated antibodies and visualized using TMB phosphate citrate substrate solution.

    Techniques: Mouse Assay, Plasmid Preparation, Enzyme-linked Immunospot, In Vitro, Recombinant

    IgG subclasses in C57BL/6J and HBeAg-Tg mice after one or two immunizations . Groups of five to seven C57BL/6J and HBeAg-Tg mice were immunized once or twice with 5 μg coHBcAg plasmid DNA in combination with 5 μg IL-12 plasmid DNA by intramuscular immunization (IVIN-EP). The groups of mice that were immunized twice had 4, 8, or 12 weeks between immunizations. Two weeks after last immunization, mice were bled and serum was tested for anti-HBc IgG subclasses (IgG1, IgG2a, IgG2b, and IgG3). All data are given as individual end point titers (black filled circles) with each groups mean titer indicated (line).

    Journal: Molecular Therapy

    Article Title: Functional Aspects of Intrahepatic Hepatitis B Virus-specific T Cells Induced by Therapeutic DNA Vaccination

    doi: 10.1038/mt.2014.233

    Figure Lengend Snippet: IgG subclasses in C57BL/6J and HBeAg-Tg mice after one or two immunizations . Groups of five to seven C57BL/6J and HBeAg-Tg mice were immunized once or twice with 5 μg coHBcAg plasmid DNA in combination with 5 μg IL-12 plasmid DNA by intramuscular immunization (IVIN-EP). The groups of mice that were immunized twice had 4, 8, or 12 weeks between immunizations. Two weeks after last immunization, mice were bled and serum was tested for anti-HBc IgG subclasses (IgG1, IgG2a, IgG2b, and IgG3). All data are given as individual end point titers (black filled circles) with each groups mean titer indicated (line).

    Article Snippet: Detection of IgG subclasses (IgG1, IgG2a, IgG2b, and IgG3) (Southern Biotech, Birmingham, AL) was performed in a similar manner as described above with the exception that serum antibodies were detected using subclass-specific anti-mouse horseradish peroxidase–conjugated antibodies and visualized using TMB phosphate citrate substrate solution.

    Techniques: Mouse Assay, Plasmid Preparation

    Rapid loss of T-cell responses after a single immunization . Groups of five C57BL/6J and HBeAg-Tg mice were immunized once with 5 μg coHBcAg plasmid DNA in combination with 5 μg IL-12 plasmid DNA by intramuscular immunization (IVIN-EP). Two, 6, 10, or 14 weeks after immunization, the mice were bled, sacrificed, and splenocytes harvested for determination of T-cell responses. In ( a ), the numbers of IFN-γ spot forming cells (SFCs) by enzyme-linked immunospot (ELISpot) assay in immunized C57BL/6J mice are shown. The ELISpot was determined after in vitro stimulation of splenocytes with peptides and recombinant proteins as indicated. Results are given as the mean SFCs/10 6 (+SD) splenocytes with a cutoff set at 50 SFCs/10 6 splenocytes. ( b ) The mean (+SD) endpoint dilution of anti-HBc IgG antibody titers 2, 6, 10, or 14 weeks after a single immunization. ( c ) Polyfunctionality (IFN-γ+, TNF-α+, CD107a+) of HBcAg-specific T-cell responses of splenocytes stimulated with overlapping peptide pools (0.3 μg/ml each peptide) covering the full-length HBcAg-protein. Each bar represents the percentage of CD8 + T cells that are polyfuntional 12 hours after antigen stimulation.

    Journal: Molecular Therapy

    Article Title: Functional Aspects of Intrahepatic Hepatitis B Virus-specific T Cells Induced by Therapeutic DNA Vaccination

    doi: 10.1038/mt.2014.233

    Figure Lengend Snippet: Rapid loss of T-cell responses after a single immunization . Groups of five C57BL/6J and HBeAg-Tg mice were immunized once with 5 μg coHBcAg plasmid DNA in combination with 5 μg IL-12 plasmid DNA by intramuscular immunization (IVIN-EP). Two, 6, 10, or 14 weeks after immunization, the mice were bled, sacrificed, and splenocytes harvested for determination of T-cell responses. In ( a ), the numbers of IFN-γ spot forming cells (SFCs) by enzyme-linked immunospot (ELISpot) assay in immunized C57BL/6J mice are shown. The ELISpot was determined after in vitro stimulation of splenocytes with peptides and recombinant proteins as indicated. Results are given as the mean SFCs/10 6 (+SD) splenocytes with a cutoff set at 50 SFCs/10 6 splenocytes. ( b ) The mean (+SD) endpoint dilution of anti-HBc IgG antibody titers 2, 6, 10, or 14 weeks after a single immunization. ( c ) Polyfunctionality (IFN-γ+, TNF-α+, CD107a+) of HBcAg-specific T-cell responses of splenocytes stimulated with overlapping peptide pools (0.3 μg/ml each peptide) covering the full-length HBcAg-protein. Each bar represents the percentage of CD8 + T cells that are polyfuntional 12 hours after antigen stimulation.

    Article Snippet: Detection of IgG subclasses (IgG1, IgG2a, IgG2b, and IgG3) (Southern Biotech, Birmingham, AL) was performed in a similar manner as described above with the exception that serum antibodies were detected using subclass-specific anti-mouse horseradish peroxidase–conjugated antibodies and visualized using TMB phosphate citrate substrate solution.

    Techniques: Mouse Assay, Plasmid Preparation, Enzyme-linked Immunospot, In Vitro, Recombinant

    IgG subtypes in the semi-immune mice strains. Serum was harvested on D0, Hm (day 16 for F1, Balb/c and day 12 for CBA) and Rec (day 28 for F1 and Balb/c). IgG subtypes (IgG1, IgG2a and IgG3) were measured (in triplicates) on the pooled sera for each semi-immune mice strains on the days serum was harvested. Values presented are the mean of the triplicates. D0 was the start of the last challenge, while Hm was when minimum Hb is attained, Rec is when parasitaemia was zero by microscopy. Mice number: F1 (5); Balb/c (5), CBA (5) and Uninfected negative control (3). D0 day zero, Hm day minimum Hb observed, Rec day parasite not detected by microscopy (parasitaemia is zero by microscopy)

    Journal: Malaria Journal

    Article Title: Elevated IL-17 levels in semi-immune anaemic mice infected with Plasmodium berghei ANKA

    doi: 10.1186/s12936-018-2257-x

    Figure Lengend Snippet: IgG subtypes in the semi-immune mice strains. Serum was harvested on D0, Hm (day 16 for F1, Balb/c and day 12 for CBA) and Rec (day 28 for F1 and Balb/c). IgG subtypes (IgG1, IgG2a and IgG3) were measured (in triplicates) on the pooled sera for each semi-immune mice strains on the days serum was harvested. Values presented are the mean of the triplicates. D0 was the start of the last challenge, while Hm was when minimum Hb is attained, Rec is when parasitaemia was zero by microscopy. Mice number: F1 (5); Balb/c (5), CBA (5) and Uninfected negative control (3). D0 day zero, Hm day minimum Hb observed, Rec day parasite not detected by microscopy (parasitaemia is zero by microscopy)

    Article Snippet: Plates were washed three times with 400 μL/well of 0.05% Tween-PBS and 100 μL of serially diluted pooled sera (1:80) was added and incubated at 37 °C for 3 h. Plates were then washed five times with 400 μL/well of 0.05% Tween-PBS, and 100 μL of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and subclasses IgG1, IgG2a and IgG3 (Southern Biotechnology, Birmingham, AL) diluted with blocking buffer (1:2500) was added and incubated for 1 h at room temperature.

    Techniques: Mouse Assay, Crocin Bleaching Assay, Microscopy, Negative Control

    Identification of the three distinct rejection phenotypes according to the characteristics of the dominant donor-specific anti-HLA antibody (MFI, HLA class specificity, C1q-binding capacity, and IgG1–4).

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgG Donor-Specific Anti-Human HLA Antibody Subclasses and Kidney Allograft Antibody-Mediated Injury

    doi: 10.1681/ASN.2014111120

    Figure Lengend Snippet: Identification of the three distinct rejection phenotypes according to the characteristics of the dominant donor-specific anti-HLA antibody (MFI, HLA class specificity, C1q-binding capacity, and IgG1–4).

    Article Snippet: The standard single-antigen assay was modified, replacing the phycoerythrin-conjugated anti-pan human IgG reporter antibody with monoclonal antibodies specific for IgG1–4 subclasses (IgG1 clone HP6001, IgG2 clone 31–7-4, IgG3 clone HP6050, IgG4 clone HP6025; Southern Biotech).

    Techniques: Binding Assay

    Distribution of IgG1–4 iDSA subclasses in the study population (Venn diagram).

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgG Donor-Specific Anti-Human HLA Antibody Subclasses and Kidney Allograft Antibody-Mediated Injury

    doi: 10.1681/ASN.2014111120

    Figure Lengend Snippet: Distribution of IgG1–4 iDSA subclasses in the study population (Venn diagram).

    Article Snippet: The standard single-antigen assay was modified, replacing the phycoerythrin-conjugated anti-pan human IgG reporter antibody with monoclonal antibodies specific for IgG1–4 subclasses (IgG1 clone HP6001, IgG2 clone 31–7-4, IgG3 clone HP6050, IgG4 clone HP6025; Southern Biotech).

    Techniques:

    MyD88 signaling is essential for B cell activation and IgG autoantibody production in BAFF Tg mice. Lethally irradiated 6-wk-old BAFF Tg mice were reconstituted with MyD88 +/+ or MyD88 −/− or mixed Rag1 −/− MyD88 +/+ or Rag1 −/− MyD88 −/− BM, as indicated. (A) Histogram of B cell activation markers CD44 (top) and CD69 (bottom) of Fo (left) and MZ (right) splenic B cells from MyD88 +/+ (solid line) and MyD88 −/− (dashed line) BAFF Tg BM chimeras. (B) Anti-dsDNA and RF antibody production in WT mice reconstituted with MyD88 +/+ (circles) or MyD88 −/− (diamonds); BAFF Tg mice reconstituted with MyD88 +/+ (squares; light gray bar); or MyD88 −/− (triangles; light gray bar); or BAFF Tg mice reconstituted with Rag1 −/− MyD88 +/+ (squares; dark gray bar); or Rag1 −/− MyD88 −/− (triangles; dark gray bar). IgG, IgA, and IgM (C) and C3 (D) deposition in the kidney of BAFF Tg mice reconstituted with MyD88 +/+ , MyD88 −/− , Rag1 −/− MyD88 +/+ , or Rag1 −/− MyD88 −/− BM, as indicated. Significant P values are shown.

    Journal: The Journal of Experimental Medicine

    Article Title: BAFF and MyD88 signals promote a lupuslike disease independent of T cells

    doi: 10.1084/jem.20062567

    Figure Lengend Snippet: MyD88 signaling is essential for B cell activation and IgG autoantibody production in BAFF Tg mice. Lethally irradiated 6-wk-old BAFF Tg mice were reconstituted with MyD88 +/+ or MyD88 −/− or mixed Rag1 −/− MyD88 +/+ or Rag1 −/− MyD88 −/− BM, as indicated. (A) Histogram of B cell activation markers CD44 (top) and CD69 (bottom) of Fo (left) and MZ (right) splenic B cells from MyD88 +/+ (solid line) and MyD88 −/− (dashed line) BAFF Tg BM chimeras. (B) Anti-dsDNA and RF antibody production in WT mice reconstituted with MyD88 +/+ (circles) or MyD88 −/− (diamonds); BAFF Tg mice reconstituted with MyD88 +/+ (squares; light gray bar); or MyD88 −/− (triangles; light gray bar); or BAFF Tg mice reconstituted with Rag1 −/− MyD88 +/+ (squares; dark gray bar); or Rag1 −/− MyD88 −/− (triangles; dark gray bar). IgG, IgA, and IgM (C) and C3 (D) deposition in the kidney of BAFF Tg mice reconstituted with MyD88 +/+ , MyD88 −/− , Rag1 −/− MyD88 +/+ , or Rag1 −/− MyD88 −/− BM, as indicated. Significant P values are shown.

    Article Snippet: Fluorescent-labeled anti–mouse antibodies CD1d, CD4, CD5, CD8, CD11c, CD21/CD35, CD25, CD44, CD45R/B220, CD62L, CD69, CD80, MHC class II, IgD, IgM, IgA, IgG subclasses (BD Biosciences) CD3, CD23, CD93 (eBioscience), BAFFR, TACI, BCMA (R & D Systems), and PDCA-1 (Miltenyi Biotec) were used for FACS and/or microscopy analysis.

    Techniques: Activation Assay, Mouse Assay, Irradiation

    TΔ-BTg and BAFF Tg mice develop indistinguishable kidney and salivary gland pathology. In A, F, and G, the mice are WT (circle), TCR −/− (diamond), BAFF Tg (square), and TΔ-BTg (triangle). Symbols represent individual mice, and the mean for each group is indicated by a column. (A) Proteinuria analysis of 12-mo-old animals. (B) HE staining of kidney tissue sections. BAFF Tg and TΔ-BTg sections show glomeruli separation (white arrows) and mononuclear cell infiltrate (black arrows). (C) IgG, IgA, and IgM (D) C3 deposition in the kidney of 8-mo-old mice. (E) HE staining of salivary gland tissue sections from BAFF Tg and TΔ-BTg sections show salivary gland destruction and lymphocyte infiltrate (black arrows). (F) Saliva flow after pilocarpine injection in 8-mo-old animals. (G) Total numbers of MZ-like B cells detected in CLN and salivary glands. (H) Isotype-specific Ig deposition in salivary gland of 8-mo-old mice. Significant P values are shown.

    Journal: The Journal of Experimental Medicine

    Article Title: BAFF and MyD88 signals promote a lupuslike disease independent of T cells

    doi: 10.1084/jem.20062567

    Figure Lengend Snippet: TΔ-BTg and BAFF Tg mice develop indistinguishable kidney and salivary gland pathology. In A, F, and G, the mice are WT (circle), TCR −/− (diamond), BAFF Tg (square), and TΔ-BTg (triangle). Symbols represent individual mice, and the mean for each group is indicated by a column. (A) Proteinuria analysis of 12-mo-old animals. (B) HE staining of kidney tissue sections. BAFF Tg and TΔ-BTg sections show glomeruli separation (white arrows) and mononuclear cell infiltrate (black arrows). (C) IgG, IgA, and IgM (D) C3 deposition in the kidney of 8-mo-old mice. (E) HE staining of salivary gland tissue sections from BAFF Tg and TΔ-BTg sections show salivary gland destruction and lymphocyte infiltrate (black arrows). (F) Saliva flow after pilocarpine injection in 8-mo-old animals. (G) Total numbers of MZ-like B cells detected in CLN and salivary glands. (H) Isotype-specific Ig deposition in salivary gland of 8-mo-old mice. Significant P values are shown.

    Article Snippet: Fluorescent-labeled anti–mouse antibodies CD1d, CD4, CD5, CD8, CD11c, CD21/CD35, CD25, CD44, CD45R/B220, CD62L, CD69, CD80, MHC class II, IgD, IgM, IgA, IgG subclasses (BD Biosciences) CD3, CD23, CD93 (eBioscience), BAFFR, TACI, BCMA (R & D Systems), and PDCA-1 (Miltenyi Biotec) were used for FACS and/or microscopy analysis.

    Techniques: Mouse Assay, Staining, Flow Cytometry, Injection

    Production of IgM and IgG, but not IgA, autoantibodies in TΔ-BTg mice. (A) ELISA was used to determine IgG, IgA, and IgM anti-ssDNA (top), anti-dsDNA (middle), and RF (bottom) in serum from 8-mo-old WT (circle), TCR −/− (diamond), BAFF Tg (square), and TΔ-BTg (triangle) mice. Symbols represent individual mice, and the mean for each group is indicated by columns. Significant P values are shown. n ≥ 6 per group. (B) Representative staining of isotype-specific ANA and anti-dsDNA were determined by staining Hep-2 and C. luciliae slides, respectively, with serum from indicated mice.

    Journal: The Journal of Experimental Medicine

    Article Title: BAFF and MyD88 signals promote a lupuslike disease independent of T cells

    doi: 10.1084/jem.20062567

    Figure Lengend Snippet: Production of IgM and IgG, but not IgA, autoantibodies in TΔ-BTg mice. (A) ELISA was used to determine IgG, IgA, and IgM anti-ssDNA (top), anti-dsDNA (middle), and RF (bottom) in serum from 8-mo-old WT (circle), TCR −/− (diamond), BAFF Tg (square), and TΔ-BTg (triangle) mice. Symbols represent individual mice, and the mean for each group is indicated by columns. Significant P values are shown. n ≥ 6 per group. (B) Representative staining of isotype-specific ANA and anti-dsDNA were determined by staining Hep-2 and C. luciliae slides, respectively, with serum from indicated mice.

    Article Snippet: Fluorescent-labeled anti–mouse antibodies CD1d, CD4, CD5, CD8, CD11c, CD21/CD35, CD25, CD44, CD45R/B220, CD62L, CD69, CD80, MHC class II, IgD, IgM, IgA, IgG subclasses (BD Biosciences) CD3, CD23, CD93 (eBioscience), BAFFR, TACI, BCMA (R & D Systems), and PDCA-1 (Miltenyi Biotec) were used for FACS and/or microscopy analysis.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Staining

    RBC autoantibody responses 6 weeks after peptide inhalation. Comparison of total IgG ( a ), IgG1 ( b ) and IgG2a ( c ) responses of individual NZB mice 6 weeks after they had inhaled PBS or peptide 25 (241–251) or peptide 29 (282–296) or both (p25 + p29). Total IgG was significantly higher in p25 and p25 + p29 groups ( P

    Journal: Auto-Immunity Highlights

    Article Title: Induction of IL-10 cytokine and the suppression of T cell proliferation by specific peptides from red cell band 3 and in vivo effects of these peptides on autoimmune hemolytic anemia in NZB mice

    doi: 10.1007/s13317-017-0095-4

    Figure Lengend Snippet: RBC autoantibody responses 6 weeks after peptide inhalation. Comparison of total IgG ( a ), IgG1 ( b ) and IgG2a ( c ) responses of individual NZB mice 6 weeks after they had inhaled PBS or peptide 25 (241–251) or peptide 29 (282–296) or both (p25 + p29). Total IgG was significantly higher in p25 and p25 + p29 groups ( P

    Article Snippet: The number of molecules of each murine IgG subclass bound to the RBC was calculated by interpolation from a standard curve generated by tanning normal RBC with a known concentration of each purified IgG subclass (Sigma).

    Techniques: Mouse Assay

    The immunogenicity of Ig idiotypes (Ids) is profoundly influenced by the isotype, Fcγ piece, and state of IgA polymerization. The y axis shows the serum levels of IgG Ab against plates coated with the homologous Id borne by IgM, determined by ELISA as described in Materials and Methods . The immunogen and the priming and booster doses are indicated at the top left parts of A – H . Each symbol represents an individual mouse. Pre, preimmunization serum; Bld, below level of detection; mono, monomer; di/poly, dimer/polymer.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Immunoglobulin heavy chain constant regions regulate immunity and tolerance to idiotypes of antibody variable regions

    doi: 10.1073/pnas.052150899

    Figure Lengend Snippet: The immunogenicity of Ig idiotypes (Ids) is profoundly influenced by the isotype, Fcγ piece, and state of IgA polymerization. The y axis shows the serum levels of IgG Ab against plates coated with the homologous Id borne by IgM, determined by ELISA as described in Materials and Methods . The immunogen and the priming and booster doses are indicated at the top left parts of A – H . Each symbol represents an individual mouse. Pre, preimmunization serum; Bld, below level of detection; mono, monomer; di/poly, dimer/polymer.

    Article Snippet: Affinity-purified unconjugated and biotin-conjugated polyclonal Abs specific for mouse IgA, IgG, and IgG subclasses were purchased from Jackson ImmunoResearch, or Southern Biotechnology Associates, or Zymed.

    Techniques: Enzyme-linked Immunosorbent Assay

    SjGP-3-specific immunoglobulin subtypes (IgG1, IgG2a and IgE) . Groups of mice were immunized with SjGP-3 formulations. Serum was collected after three time immunization. IgG1, IgG2a and IgE titers were detected by ELISA in triplicate wells respectively and the IgG2a to IgG1 ratio was calculated. (A) Anti-SjGP-3 antibody titers. (B) IgG2a/IgG1 ratio. * p

    Journal: BMC Infectious Diseases

    Article Title: A Schistosoma japonicum chimeric protein with a novel adjuvant induced a polarized Th1 immune response and protection against liver egg burdens

    doi: 10.1186/1471-2334-9-54

    Figure Lengend Snippet: SjGP-3-specific immunoglobulin subtypes (IgG1, IgG2a and IgE) . Groups of mice were immunized with SjGP-3 formulations. Serum was collected after three time immunization. IgG1, IgG2a and IgE titers were detected by ELISA in triplicate wells respectively and the IgG2a to IgG1 ratio was calculated. (A) Anti-SjGP-3 antibody titers. (B) IgG2a/IgG1 ratio. * p

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) was performed to detect the levels of different isotypes against the fusion or individual proteins using HRP-labeled goat antibodies specific for IgG, IgE and IgG subclasses (Bio-Rad, USA).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    RIA quantitation of HBsAg levels in the supernatants of PLC/PRF/5 cells cultured with human monoclonal anti-HBs IgG. During the first period (open bars), the cells were cultured in the presence of medium with FCS and two concentrations of anti-HBs. In parallel, cells were cultured with medium plus FCS only, i.e., without human IgG (FCS) or with nonimmune human IgG (ABS), as controls. After 2 days of culture, the supernatants were collected, and the same cells were maintained in culture for a further two intervals of 2 days each (shaded and solid bars, respectively) in medium with FCS without human IgG. The HBsAg levels in the supernatants were tested at the end of each period, i.e., at three time points. The bars represent the means and standard deviations of two separate experiments, with each experimental condition run in duplicate.

    Journal: Journal of Virology

    Article Title: Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

    doi: 10.1128/JVI.77.16.8882-8892.2003

    Figure Lengend Snippet: RIA quantitation of HBsAg levels in the supernatants of PLC/PRF/5 cells cultured with human monoclonal anti-HBs IgG. During the first period (open bars), the cells were cultured in the presence of medium with FCS and two concentrations of anti-HBs. In parallel, cells were cultured with medium plus FCS only, i.e., without human IgG (FCS) or with nonimmune human IgG (ABS), as controls. After 2 days of culture, the supernatants were collected, and the same cells were maintained in culture for a further two intervals of 2 days each (shaded and solid bars, respectively) in medium with FCS without human IgG. The HBsAg levels in the supernatants were tested at the end of each period, i.e., at three time points. The bars represent the means and standard deviations of two separate experiments, with each experimental condition run in duplicate.

    Article Snippet: Two antibodies against epitopes within the a -determinant of HBsAg were used: humanized monoclonal anti-HBs of the IgG1 subclass (BM 80.1003; Boehringer Mannheim, Mannheim, Germany) and human polyclonal anti-HBs IgG (HBIG; BioProducts Laboratory, Elstree, United Kingdom).

    Techniques: Quantitation Assay, Planar Chromatography, Cell Culture

    Immunoblot analysis of cytoplasmic extracts from PLC/PRF/5 cells cultured with different concentrations of polyclonal anti-HBs IgG (HBIG). The cells were cultured in medium without human IgG (lanes 1), with 0.2 mg of HBIG/ml (lanes 2), with 1.0 mg of HBIG/ml (lanes 3), or with 2.0 mg of HBIG/ml (lanes 4). (a) Amido black staining of proteins. (b) Detection of human IgG. (c) Detection of HBsAg. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units. The arrows indicate the signals for the γ chain of IgG (50 kDa) and for both forms of HBsAg (24 and 27 kDA). LM, protein length marker.

    Journal: Journal of Virology

    Article Title: Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

    doi: 10.1128/JVI.77.16.8882-8892.2003

    Figure Lengend Snippet: Immunoblot analysis of cytoplasmic extracts from PLC/PRF/5 cells cultured with different concentrations of polyclonal anti-HBs IgG (HBIG). The cells were cultured in medium without human IgG (lanes 1), with 0.2 mg of HBIG/ml (lanes 2), with 1.0 mg of HBIG/ml (lanes 3), or with 2.0 mg of HBIG/ml (lanes 4). (a) Amido black staining of proteins. (b) Detection of human IgG. (c) Detection of HBsAg. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units. The arrows indicate the signals for the γ chain of IgG (50 kDa) and for both forms of HBsAg (24 and 27 kDA). LM, protein length marker.

    Article Snippet: Two antibodies against epitopes within the a -determinant of HBsAg were used: humanized monoclonal anti-HBs of the IgG1 subclass (BM 80.1003; Boehringer Mannheim, Mannheim, Germany) and human polyclonal anti-HBs IgG (HBIG; BioProducts Laboratory, Elstree, United Kingdom).

    Techniques: Planar Chromatography, Cell Culture, Staining, Marker

    Detection of human IgG and FcRn receptor by immunocytochemistry and light microscopy (a to c; magnification, ×65,000) or by IEM (d to f; magnification, ×72,000). (a, b, d, and e) Detection of IgG in HuH-7 cells cultured in the absence of human IgG (a), in the presence of 1.0 mg of polyclonal anti-HBs IgG/ml (b and e), or in medium containing human AB serum (d). (c and f) Detection of human FcRn. Hematoxylin staining of nuclei (light grey) and peroxidase staining (black) are shown. The arrows (the short arrows indicate the cellular membrane; the long arrows indicate membranous invaginations) indicate the positive signals for IgG or FcRn.

    Journal: Journal of Virology

    Article Title: Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

    doi: 10.1128/JVI.77.16.8882-8892.2003

    Figure Lengend Snippet: Detection of human IgG and FcRn receptor by immunocytochemistry and light microscopy (a to c; magnification, ×65,000) or by IEM (d to f; magnification, ×72,000). (a, b, d, and e) Detection of IgG in HuH-7 cells cultured in the absence of human IgG (a), in the presence of 1.0 mg of polyclonal anti-HBs IgG/ml (b and e), or in medium containing human AB serum (d). (c and f) Detection of human FcRn. Hematoxylin staining of nuclei (light grey) and peroxidase staining (black) are shown. The arrows (the short arrows indicate the cellular membrane; the long arrows indicate membranous invaginations) indicate the positive signals for IgG or FcRn.

    Article Snippet: Two antibodies against epitopes within the a -determinant of HBsAg were used: humanized monoclonal anti-HBs of the IgG1 subclass (BM 80.1003; Boehringer Mannheim, Mannheim, Germany) and human polyclonal anti-HBs IgG (HBIG; BioProducts Laboratory, Elstree, United Kingdom).

    Techniques: Immunocytochemistry, Light Microscopy, Cell Culture, Staining

    Quantification of HBsAg levels in the culture supernatants of HuH-7 cells transfected with HBV genomes expressing wild-type HBsAg (solid bars) or mutant G145R HBsAg (open bars). (a) HBV-transfected cells were cultured with FCS only as a control (c), with 1.0 mg of human monoclonal anti-HBs IgG/ml, or with 1.0 mg of human anti-HBc IgG/ml. (b) In another experiment, HuH-7 cells were transfected in parallel with the wild-ype HBV or G145R mutant HBV and cultured with 1.0 mg of monoclonal anti-HBs IgG/ml (mAb) or with 1.0 mg of polyclonal anti-HBs IgG/ml (pAb). The bars represent the means and standard deviations of duplicate samples.

    Journal: Journal of Virology

    Article Title: Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

    doi: 10.1128/JVI.77.16.8882-8892.2003

    Figure Lengend Snippet: Quantification of HBsAg levels in the culture supernatants of HuH-7 cells transfected with HBV genomes expressing wild-type HBsAg (solid bars) or mutant G145R HBsAg (open bars). (a) HBV-transfected cells were cultured with FCS only as a control (c), with 1.0 mg of human monoclonal anti-HBs IgG/ml, or with 1.0 mg of human anti-HBc IgG/ml. (b) In another experiment, HuH-7 cells were transfected in parallel with the wild-ype HBV or G145R mutant HBV and cultured with 1.0 mg of monoclonal anti-HBs IgG/ml (mAb) or with 1.0 mg of polyclonal anti-HBs IgG/ml (pAb). The bars represent the means and standard deviations of duplicate samples.

    Article Snippet: Two antibodies against epitopes within the a -determinant of HBsAg were used: humanized monoclonal anti-HBs of the IgG1 subclass (BM 80.1003; Boehringer Mannheim, Mannheim, Germany) and human polyclonal anti-HBs IgG (HBIG; BioProducts Laboratory, Elstree, United Kingdom).

    Techniques: Transfection, Expressing, Mutagenesis, Cell Culture

    Immunoblot of human IgG in cytoplasmic extracts from PLC/PRF/5 cells (lanes 1 to 4) and HuH-7 cells (lanes 5 to 8). The cells were cultured with medium containing human AB serum (lanes 1 and 5), human AB serum plus 1.0 mg of monoclonal anti-HBs IgG/ml (lanes 2 and 6), medium with FCS alone (lanes 3 and 7), or 1.0 mg of monoclonal anti-HBs IgG/ml (lanes 4 and 8). As a standard, monoclonal anti-HBs IgG (mAb) was included in the blot. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units (au). Cellular IgG, estimated amount of IgG associated with cells; IgG uptake, relative amount of IgG associated with the cells as a percentage of the IgG in culture supernatants. The amount of the latter was calculated on the basis of the known IgG concentration (1.0 mg/ml) and the known volume of medium (2.5 ml) in each well, i.e., 2,500 μg of IgG.

    Journal: Journal of Virology

    Article Title: Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

    doi: 10.1128/JVI.77.16.8882-8892.2003

    Figure Lengend Snippet: Immunoblot of human IgG in cytoplasmic extracts from PLC/PRF/5 cells (lanes 1 to 4) and HuH-7 cells (lanes 5 to 8). The cells were cultured with medium containing human AB serum (lanes 1 and 5), human AB serum plus 1.0 mg of monoclonal anti-HBs IgG/ml (lanes 2 and 6), medium with FCS alone (lanes 3 and 7), or 1.0 mg of monoclonal anti-HBs IgG/ml (lanes 4 and 8). As a standard, monoclonal anti-HBs IgG (mAb) was included in the blot. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units (au). Cellular IgG, estimated amount of IgG associated with cells; IgG uptake, relative amount of IgG associated with the cells as a percentage of the IgG in culture supernatants. The amount of the latter was calculated on the basis of the known IgG concentration (1.0 mg/ml) and the known volume of medium (2.5 ml) in each well, i.e., 2,500 μg of IgG.

    Article Snippet: Two antibodies against epitopes within the a -determinant of HBsAg were used: humanized monoclonal anti-HBs of the IgG1 subclass (BM 80.1003; Boehringer Mannheim, Mannheim, Germany) and human polyclonal anti-HBs IgG (HBIG; BioProducts Laboratory, Elstree, United Kingdom).

    Techniques: Planar Chromatography, Cell Culture, Concentration Assay

    Detection of HBV DNA in supernatants and cytoplasmic extracts of HepG2.215 cells. (a) Quantitative real-time PCR for the detection of HBV DNA in cell culture supernatants. HepG2.2.15 cells were cultured for an initial period (open bars) without human IgG (FCS), with nonimmune IgG (ABS), or with 0.1 or 1.0 mg of monoclonal HBs-specific IgG/ml (anti-HBs). In the second period (solid bars), the same cells were maintained in culture for a further two time intervals without human IgG. The bars represent the means and the standard deviations of duplicate samples. (b) Southern blot hybridization for the detection of HBV replicative intermediates in the cytoplasm of HepG2.215 cells cultured without human IgG (FCS), with nonimmune human IgG (ABS), or with monoclonal anti-HBs (mAb). The arrows indicate the signals for single-stranded (ss) and double-stranded (ds) HBV DNAs. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units.

    Journal: Journal of Virology

    Article Title: Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

    doi: 10.1128/JVI.77.16.8882-8892.2003

    Figure Lengend Snippet: Detection of HBV DNA in supernatants and cytoplasmic extracts of HepG2.215 cells. (a) Quantitative real-time PCR for the detection of HBV DNA in cell culture supernatants. HepG2.2.15 cells were cultured for an initial period (open bars) without human IgG (FCS), with nonimmune IgG (ABS), or with 0.1 or 1.0 mg of monoclonal HBs-specific IgG/ml (anti-HBs). In the second period (solid bars), the same cells were maintained in culture for a further two time intervals without human IgG. The bars represent the means and the standard deviations of duplicate samples. (b) Southern blot hybridization for the detection of HBV replicative intermediates in the cytoplasm of HepG2.215 cells cultured without human IgG (FCS), with nonimmune human IgG (ABS), or with monoclonal anti-HBs (mAb). The arrows indicate the signals for single-stranded (ss) and double-stranded (ds) HBV DNAs. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units.

    Article Snippet: Two antibodies against epitopes within the a -determinant of HBsAg were used: humanized monoclonal anti-HBs of the IgG1 subclass (BM 80.1003; Boehringer Mannheim, Mannheim, Germany) and human polyclonal anti-HBs IgG (HBIG; BioProducts Laboratory, Elstree, United Kingdom).

    Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Southern Blot, Hybridization

    Immunoblot analysis of cytoplasmic extracts from PLC/PRF/5 cells cultured in the presence of different concentrations of human monoclonal anti-HBs. The cells were cultured in medium with 0.2 mg of anti-HBs/ml (lanes 1), with 1.0 mg of anti-HBs/ml (lanes 2), with 2.0 mg of anti-HBs/ml (lanes 3), or without human IgG (lanes 4). (a) Amido black staining of proteins. (b) Detection of human IgG. (c) Detection of HBsAg. (d) Detection of FcRn. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units. The arrows indicate the signals for the γ chain of IgG (50 kDa), for both forms of HBsAg (24 and 27 kDa), and for the heavy chain of FcRn (45 kDa). LM, protein length marker.

    Journal: Journal of Virology

    Article Title: Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

    doi: 10.1128/JVI.77.16.8882-8892.2003

    Figure Lengend Snippet: Immunoblot analysis of cytoplasmic extracts from PLC/PRF/5 cells cultured in the presence of different concentrations of human monoclonal anti-HBs. The cells were cultured in medium with 0.2 mg of anti-HBs/ml (lanes 1), with 1.0 mg of anti-HBs/ml (lanes 2), with 2.0 mg of anti-HBs/ml (lanes 3), or without human IgG (lanes 4). (a) Amido black staining of proteins. (b) Detection of human IgG. (c) Detection of HBsAg. (d) Detection of FcRn. INT, intensity of the chemiluminescence signal measured by FluorS MultiImager in arbitrary units. The arrows indicate the signals for the γ chain of IgG (50 kDa), for both forms of HBsAg (24 and 27 kDa), and for the heavy chain of FcRn (45 kDa). LM, protein length marker.

    Article Snippet: Two antibodies against epitopes within the a -determinant of HBsAg were used: humanized monoclonal anti-HBs of the IgG1 subclass (BM 80.1003; Boehringer Mannheim, Mannheim, Germany) and human polyclonal anti-HBs IgG (HBIG; BioProducts Laboratory, Elstree, United Kingdom).

    Techniques: Planar Chromatography, Cell Culture, Staining, Marker

    IgM, IgG and IgG subclasses of alloantibodies to MHC class I antigens measured by flow cytometry in serum from C57BL/6 WT (square symbols) and FcγRIII-KO recipients (circle symbols) 7 days after transplantation FcγRIII-KO demonstrated higher levels of IgM (A) and IgG (B) alloantibodies to MHC class I antigens in the circulation than in WT recipients (titer > 64). IgG was mainly composed of C-activating IgG2a and IgG2b subclasses (C, D). The level of non-C activating IgG1 was very low in both FcγRIII-KO and WT recipients (E). Alloantibodies from FcγRIII-KO mice showed significantly higher ability to activate C and deposit C4d on target cells than their WT counterparts (F). Each point represents the average mode channel fluorescence staining +SD (n > 10). Statistically significant differences (p

    Journal: Transplantation

    Article Title: The absence of FcγRIII results in increased pro-inflammatory response in FcγRIII-KO cardiac recipients

    doi: 10.1097/TP.0b013e31829c2455

    Figure Lengend Snippet: IgM, IgG and IgG subclasses of alloantibodies to MHC class I antigens measured by flow cytometry in serum from C57BL/6 WT (square symbols) and FcγRIII-KO recipients (circle symbols) 7 days after transplantation FcγRIII-KO demonstrated higher levels of IgM (A) and IgG (B) alloantibodies to MHC class I antigens in the circulation than in WT recipients (titer > 64). IgG was mainly composed of C-activating IgG2a and IgG2b subclasses (C, D). The level of non-C activating IgG1 was very low in both FcγRIII-KO and WT recipients (E). Alloantibodies from FcγRIII-KO mice showed significantly higher ability to activate C and deposit C4d on target cells than their WT counterparts (F). Each point represents the average mode channel fluorescence staining +SD (n > 10). Statistically significant differences (p

    Article Snippet: To analyze the IgG subclass of alloantibodies, cells were washed after the first incubation with diluted sera and then reacted with 50 μl of PBA containing an optimal dilution of FITC-conjugated rat mAb against mouse IgG1, IgG2a and IgG2b subclasses (Pharmingen, CA).

    Techniques: Flow Cytometry, Cytometry, Transplantation Assay, Mouse Assay, Fluorescence, Staining

    Induction of specific immune responses in IL-4Rα −/− mice receiving UreA-specific CD4 + T cells. (A) Proliferation of splenic CD4 + T cells isolated from recipient challenged or nonrecipient infected mice. Results are expressed as mean ± standard error of the mean. (B) Analysis of IgG2a and IgG1 antibody responses in serum. Results are expressed as the mean ± standard deviation. IgG2a titers in recipient mice were significantly higher than in nonrecipient mice (∗) ( P

    Journal: Infection and Immunity

    Article Title: Adoptive Transfer of CD4+ T Cells Specific for Subunit A of Helicobacter pylori Urease Reduces H. pylori Stomach Colonization in Mice in the Absence of Interleukin-4 (IL-4)/IL-13 Receptor Signaling

    doi: 10.1128/IAI.69.3.1714-1721.2001

    Figure Lengend Snippet: Induction of specific immune responses in IL-4Rα −/− mice receiving UreA-specific CD4 + T cells. (A) Proliferation of splenic CD4 + T cells isolated from recipient challenged or nonrecipient infected mice. Results are expressed as mean ± standard error of the mean. (B) Analysis of IgG2a and IgG1 antibody responses in serum. Results are expressed as the mean ± standard deviation. IgG2a titers in recipient mice were significantly higher than in nonrecipient mice (∗) ( P

    Article Snippet: Bound specific antibodies were detected with goat anti-mouse IgG2a or goat anti-mouse IgG1 conjugated to horseradish peroxidase, used at a dilution of 1/5,000 (Nordic Immunological Laboratories, Tilburg, The Netherlands).

    Techniques: Mouse Assay, Isolation, Infection, Standard Deviation

    Mice which received UreA-specific CD4 + T cells mounted IgG2a and IgG1 antibody responses against urease. Sera were taken 6 weeks after infection. Results are expressed as mean ± standard deviation. Groups contained five to seven mice. Statistically significant differences (two-tailed t test) between recipient and nonrecipient mice were calculated for IgG2a ( P > 0.1) and IgG1 ( P

    Journal: Infection and Immunity

    Article Title: Adoptive Transfer of CD4+ T Cells Specific for Subunit A of Helicobacter pylori Urease Reduces H. pylori Stomach Colonization in Mice in the Absence of Interleukin-4 (IL-4)/IL-13 Receptor Signaling

    doi: 10.1128/IAI.69.3.1714-1721.2001

    Figure Lengend Snippet: Mice which received UreA-specific CD4 + T cells mounted IgG2a and IgG1 antibody responses against urease. Sera were taken 6 weeks after infection. Results are expressed as mean ± standard deviation. Groups contained five to seven mice. Statistically significant differences (two-tailed t test) between recipient and nonrecipient mice were calculated for IgG2a ( P > 0.1) and IgG1 ( P

    Article Snippet: Bound specific antibodies were detected with goat anti-mouse IgG2a or goat anti-mouse IgG1 conjugated to horseradish peroxidase, used at a dilution of 1/5,000 (Nordic Immunological Laboratories, Tilburg, The Netherlands).

    Techniques: Mouse Assay, Infection, Standard Deviation, Two Tailed Test

    Effects of G M1 and Aβ-(1–40) on NSCs ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control IgG (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).

    Journal: ASN NEURO

    Article Title: Cytotoxic effects of GM1 ganglioside and amyloid ?-peptide on mouse embryonic neural stem cells

    doi: 10.1042/AN20090063

    Figure Lengend Snippet: Effects of G M1 and Aβ-(1–40) on NSCs ( A ) NSCs prepared from striata of mouse embryos in the form of neurospheres at 0, 3 and 7 days in vitro (DIV). ( B ) NSCs stained with subclass control IgG (coIgG) or anti-nestin antibody. Nuclei were stained with Hoechst 33258. ( C ) Apoptotic cells in NSCs treated with G M1 (0 or 40 μM) and Aβ-(1–40) (0 or 10 μM) in the presence of bFGF (5 ng/ml) for 3 days were detected with the TUNEL assay. Nuclei were stained with Hoechst 33258. ( D ) The proportion of TUNEL-positive cells in NSCs treated with or without G M1 and Aβ-(1–40).

    Article Snippet: Confirmation of NSCs was performed by cell staining using a subclass control IgG (BD Biosciences) or anti-nestin monoclonal antibody.

    Techniques: In Vitro, Staining, TUNEL Assay

    Mean relative abundance of the percentage of G 0 F relative to the total glycoform abundance in the antibody subclasses among the groups of myositis patients, asymptomatic siblings, and unrelated age-matched controls. A) IgG 1 subclass and B) IgG 2–3

    Journal: Journal of proteome research

    Article Title: Mass Spectrometric Determination of IgG Subclass-Specific Glycosylation Profiles in Siblings Discordant for Myositis Syndromes

    doi: 10.1021/pr200397h

    Figure Lengend Snippet: Mean relative abundance of the percentage of G 0 F relative to the total glycoform abundance in the antibody subclasses among the groups of myositis patients, asymptomatic siblings, and unrelated age-matched controls. A) IgG 1 subclass and B) IgG 2–3

    Article Snippet: To more easily visualize these data, a bar graph representing the relative abundance of each glycoform of the IgG1 subclass observed in one plasma sample set is shown in .

    Techniques:

    Mean relative abundance of subclass specific IgG glycosylation profiles. A) IgG 1 subclass and B) IgG 2-3 subclass. Solid black bars – controls, hatched bars – siblings, solid white bars – myositis patients. The eleven glycoforms

    Journal: Journal of proteome research

    Article Title: Mass Spectrometric Determination of IgG Subclass-Specific Glycosylation Profiles in Siblings Discordant for Myositis Syndromes

    doi: 10.1021/pr200397h

    Figure Lengend Snippet: Mean relative abundance of subclass specific IgG glycosylation profiles. A) IgG 1 subclass and B) IgG 2-3 subclass. Solid black bars – controls, hatched bars – siblings, solid white bars – myositis patients. The eleven glycoforms

    Article Snippet: To more easily visualize these data, a bar graph representing the relative abundance of each glycoform of the IgG1 subclass observed in one plasma sample set is shown in .

    Techniques:

    A) Representative extracted ion current (EIC) of the ion of m/z 204.1 over the chromatographic time window 20–60 minutes from the LC-MS analysis of the total IgG heavy chain digest from one myositis patient. These data show the characteristic

    Journal: Journal of proteome research

    Article Title: Mass Spectrometric Determination of IgG Subclass-Specific Glycosylation Profiles in Siblings Discordant for Myositis Syndromes

    doi: 10.1021/pr200397h

    Figure Lengend Snippet: A) Representative extracted ion current (EIC) of the ion of m/z 204.1 over the chromatographic time window 20–60 minutes from the LC-MS analysis of the total IgG heavy chain digest from one myositis patient. These data show the characteristic

    Article Snippet: To more easily visualize these data, a bar graph representing the relative abundance of each glycoform of the IgG1 subclass observed in one plasma sample set is shown in .

    Techniques: Liquid Chromatography with Mass Spectroscopy

    Depletion of CD301b + DCs leads to enhanced antibody responses to the antigen immunized with weak or no adjuvant. ( a ) Immunization and sample collection timeline. ( b ) Mgl2-DTR mice were treated with 0.5 µg DT or its inactive mutant CRM197 (CRM) on days -1 and +2 and immunized on day 0 with 50 µg papain plus indicated amount of OVA in the footpad. All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and sera were harvested on day 21 for OVA-specific antibody ELISA. ( c ) WT and Mgl2-DTR mice were treated with 0.5 µg DT on days −1 and +2 and immunized in the footpad with 5 µg OVA without any adjuvant on day 0 (top). Alternatively, lethally-irradiated WT mice were reconstituted with Mgl2-DTR (DTR only), WT (WT only) or 1:1-mixture of WT and Mgl2-DTR (WT+DTR) BM cells, then immunized with 5 µg OVA without adjuvant (bottom). All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and day 21, and sera were harvested on day 21 and day 28 for OVA-specific IgG1 ELISA. Bars indicate mean ± S.E.M. calculated from 4–11 individual mice. n.s., not significant, *p

    Journal: eLife

    Article Title: CD301b+ dendritic cells suppress T follicular helper cells and antibody responses to protein antigens

    doi: 10.7554/eLife.17979

    Figure Lengend Snippet: Depletion of CD301b + DCs leads to enhanced antibody responses to the antigen immunized with weak or no adjuvant. ( a ) Immunization and sample collection timeline. ( b ) Mgl2-DTR mice were treated with 0.5 µg DT or its inactive mutant CRM197 (CRM) on days -1 and +2 and immunized on day 0 with 50 µg papain plus indicated amount of OVA in the footpad. All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and sera were harvested on day 21 for OVA-specific antibody ELISA. ( c ) WT and Mgl2-DTR mice were treated with 0.5 µg DT on days −1 and +2 and immunized in the footpad with 5 µg OVA without any adjuvant on day 0 (top). Alternatively, lethally-irradiated WT mice were reconstituted with Mgl2-DTR (DTR only), WT (WT only) or 1:1-mixture of WT and Mgl2-DTR (WT+DTR) BM cells, then immunized with 5 µg OVA without adjuvant (bottom). All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and day 21, and sera were harvested on day 21 and day 28 for OVA-specific IgG1 ELISA. Bars indicate mean ± S.E.M. calculated from 4–11 individual mice. n.s., not significant, *p

    Article Snippet: Subclass-specific antibodies that bound to OVA were detected by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgM (Southern Biotech), IgG1, IgG2b or subclass-nonspecific IgG (Jackson ImmunoResearch Laboratories, West Grove, PA) or by biotinylated anti-mouse IgE (RME-1, BioLegend) followed by HRP-streptavidin.

    Techniques: Mouse Assay, Mutagenesis, Injection, Enzyme-linked Immunosorbent Assay, Irradiation

    Depletion of CD301b + DCs enhances antibody responses to type 1 immunization when adjuvant is limited. WT and Mgl2-DTR mice were treated with 0.5 µg DT on days −1 and +2 and immunized in the footpad with 5 µg OVA plus indicated amount of CpG2216 or LPS as in a . All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and sera were harvested on day 21 for OVA-specific IgG2b ELISA. Sera were collected in two to four independent experiments. Bars indicate mean ± S.E.M. calculated from 6–7 ( b ) or 4–11 ( c ) individual mice. n.s., not significant, *p

    Journal: eLife

    Article Title: CD301b+ dendritic cells suppress T follicular helper cells and antibody responses to protein antigens

    doi: 10.7554/eLife.17979

    Figure Lengend Snippet: Depletion of CD301b + DCs enhances antibody responses to type 1 immunization when adjuvant is limited. WT and Mgl2-DTR mice were treated with 0.5 µg DT on days −1 and +2 and immunized in the footpad with 5 µg OVA plus indicated amount of CpG2216 or LPS as in a . All mice received i.p. injection of 10 µg OVA without adjuvant on day 14 and sera were harvested on day 21 for OVA-specific IgG2b ELISA. Sera were collected in two to four independent experiments. Bars indicate mean ± S.E.M. calculated from 6–7 ( b ) or 4–11 ( c ) individual mice. n.s., not significant, *p

    Article Snippet: Subclass-specific antibodies that bound to OVA were detected by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgM (Southern Biotech), IgG1, IgG2b or subclass-nonspecific IgG (Jackson ImmunoResearch Laboratories, West Grove, PA) or by biotinylated anti-mouse IgE (RME-1, BioLegend) followed by HRP-streptavidin.

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    IgG3 production in mice immunized with OVA plus papain in the footpad. All mice received intraperitoneal injection of OVA without papain on day 14.

    Journal: eLife

    Article Title: CD301b+ dendritic cells suppress T follicular helper cells and antibody responses to protein antigens

    doi: 10.7554/eLife.17979

    Figure Lengend Snippet: IgG3 production in mice immunized with OVA plus papain in the footpad. All mice received intraperitoneal injection of OVA without papain on day 14.

    Article Snippet: Subclass-specific antibodies that bound to OVA were detected by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgM (Southern Biotech), IgG1, IgG2b or subclass-nonspecific IgG (Jackson ImmunoResearch Laboratories, West Grove, PA) or by biotinylated anti-mouse IgE (RME-1, BioLegend) followed by HRP-streptavidin.

    Techniques: Mouse Assay, Injection

    Depletion of CD301b + DCs enhances autoreactive antibodies. WT or Mgl2-DTR mice were treated i.p. with PBS or 0.5 µg DT every third day for 9 days as in a . Sera were harvested at day 60 and examined for anti-nuclear IgG antibodies by ELISA ( b ). Bars indicate mean ± S.E.M. *p

    Journal: eLife

    Article Title: CD301b+ dendritic cells suppress T follicular helper cells and antibody responses to protein antigens

    doi: 10.7554/eLife.17979

    Figure Lengend Snippet: Depletion of CD301b + DCs enhances autoreactive antibodies. WT or Mgl2-DTR mice were treated i.p. with PBS or 0.5 µg DT every third day for 9 days as in a . Sera were harvested at day 60 and examined for anti-nuclear IgG antibodies by ELISA ( b ). Bars indicate mean ± S.E.M. *p

    Article Snippet: Subclass-specific antibodies that bound to OVA were detected by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgM (Southern Biotech), IgG1, IgG2b or subclass-nonspecific IgG (Jackson ImmunoResearch Laboratories, West Grove, PA) or by biotinylated anti-mouse IgE (RME-1, BioLegend) followed by HRP-streptavidin.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay