igg enzyme linked immunosorbent assay elisa Search Results


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  • 93
    Thermo Fisher enzyme linked immunosorbent assay
    Enzyme Linked Immunosorbent Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    InBios International igg enzyme linked immunosorbent assay elisa
    Igg Enzyme Linked Immunosorbent Assay Elisa, supplied by InBios International, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson igg enzyme linked immunosorbent assays
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Igg Enzyme Linked Immunosorbent Assays, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EUROIMMUN microplate enzyme linked immunosorbent assay elisa
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Microplate Enzyme Linked Immunosorbent Assay Elisa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biohit pylori igg enzyme linked immunosorbent assay
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Pylori Igg Enzyme Linked Immunosorbent Assay, supplied by Biohit, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bethyl igg enzyme linked immunosorbent assay elisa kits
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Igg Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    VECTOR-BEST igg enzyme linked immunosorbent assay elisa kits
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Igg Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by VECTOR-BEST, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mab Technologies igg enzyme linked immunosorbent assay elisa kit
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Igg Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Mab Technologies, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Standard Diagnostics Inc igg capture enzyme linked immunosorbent assay elisa kits
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Igg Capture Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Standard Diagnostics Inc, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bethyl igg quantitation enzyme linked immunosorbent assay
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Igg Quantitation Enzyme Linked Immunosorbent Assay, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Trinity Biotech igg enzyme linked immunosorbent assay
    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive <t>IgG</t> by enzyme-linked <t>immunosorbent</t> assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P
    Igg Enzyme Linked Immunosorbent Assay, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ZeptoMetrix igg enzyme linked immunosorbent assay elisa kits
    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G <t>(IgG)</t> antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked <t>immunosorbent</t> spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay <t>(ELISA)</t> with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.
    Igg Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by ZeptoMetrix, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Focus Diagnostics Inc herpeselect enzyme linked immunosorbent assay elisa igg kits
    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G <t>(IgG)</t> antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked <t>immunosorbent</t> spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay <t>(ELISA)</t> with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.
    Herpeselect Enzyme Linked Immunosorbent Assay Elisa Igg Kits, supplied by Focus Diagnostics Inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbott Laboratories zeus igg enzyme linked immunosorbent assay elisa kits
    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G <t>(IgG)</t> antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked <t>immunosorbent</t> spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay <t>(ELISA)</t> with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.
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    Bio-Quant rubella igg enzyme linked immunosorbent assay elisa
    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G <t>(IgG)</t> antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked <t>immunosorbent</t> spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay <t>(ELISA)</t> with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.
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    Biohit immunoglobulin g enzyme linked immunosorbent assay elisa
    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G <t>(IgG)</t> antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked <t>immunosorbent</t> spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay <t>(ELISA)</t> with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.
    Immunoglobulin G Enzyme Linked Immunosorbent Assay Elisa, supplied by Biohit, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bethyl igg2a enzyme linked immunosorbent assay elisa quantitation kit
    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and <t>IgG2a</t> levels were determined by <t>ELISA.</t> Data are expressed as means ± SDs. * P
    Igg2a Enzyme Linked Immunosorbent Assay Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Diagnostic Automation Inc pylori specific igg enzyme linked immunosorbent assay elisa
    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and <t>IgG2a</t> levels were determined by <t>ELISA.</t> Data are expressed as means ± SDs. * P
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    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and <t>IgG2a</t> levels were determined by <t>ELISA.</t> Data are expressed as means ± SDs. * P
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    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and <t>IgG2a</t> levels were determined by <t>ELISA.</t> Data are expressed as means ± SDs. * P
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    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and <t>IgG2a</t> levels were determined by <t>ELISA.</t> Data are expressed as means ± SDs. * P
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    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and <t>IgG2a</t> levels were determined by <t>ELISA.</t> Data are expressed as means ± SDs. * P
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    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and <t>IgG2a</t> levels were determined by <t>ELISA.</t> Data are expressed as means ± SDs. * P
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    UV-inactivated MV-NIS remains antigenic. Notes: MV-NIS was inactivated by 0.5 Joule/cm 2 UV-C light for 0.5 h. UV-inactivated MV-NIS was serially diluted in the presence of 2% measles immune serum and pre-incubated for 0.5 h at room temperature. An <t>ELISA</t> was performed using plates coated with MV antigens. Optical density at 450 nm (OD 450 ) was measured to determine the amount of anti-MV IgGs that were initially not caught by the decoy virus and could therefore bind to the coated MV antigens. Technical positive and negative controls were run in parallel. Abbreviations: ELISA, enzyme-linked <t>immunosorbent</t> assay; UV, ultraviolet; UV-C, short-wavelength ultraviolet light; MV-NIS, human sodium iodide symporter measles virus; <t>IgG,</t> immunoglobulin G; OD, optical density; Pos, positive; Neg, negative.
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    Image Search Results


    Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive IgG by enzyme-linked immunosorbent assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P

    Journal: Nature Communications

    Article Title: TCR ITAM multiplicity is required for the generation of follicular helper T-cells

    doi: 10.1038/ncomms7982

    Figure Lengend Snippet: Impaired induction of T follicular helper (TFH) cells in 6F/6F mice. 6Y/6Y and 6F/6F mice were injected with sheep red blood cells (SRBCS) and 10 days later spleens were harvested for analysis. Percentage and numbers of ( a ) TFH (CD4 + CXCR5 + PD-1 + ) cells and ( b ) germinal centre B (GC B) cells (B220 + Fas + GL-7 + ) detected in 6Y/6Y and 6F/6F mice. N =9 6Y/6Y and 9 6F/6F mice. ( c ) Immunofluorescence staining of spleens from 6Y/6Y and 6F/6F mice 1 week after immunization with SRBCS. One representative of three experiments. Scale bar, 200 μm. ( d ) Mean fluorescence intensity of anti-Bcl6 mAb staining of conventional CD4-SP T-cells (CD4 + CXCR5 − PD-1 − ) and TFH cells (CD4 + CXCR5 + PD-1 + ). ( e – g ) 6Y/6Y and 6F/6F mice (six each) were immunized with NP-KLH or NP-CGG. One week after immunization, serum was obtained by tail bleeding and analysed for NP-reactive IgG by enzyme-linked immunosorbent assay (ELISA). ( e ) Anti-NP IgG1. ( f ) Anti-NP IgG2a. ( g ) Ratio of high affinity/low affinity anti-NP IgG1 determined by ELISA with plates coated with NP(4) or NP(32). For bar graphs, data were analysed by unpaired t -test (two tailed) and are represented as mean±s.d. * P

    Article Snippet: IgM and IgG enzyme-linked immunosorbent assays (BD Biosciences) were performed with NP4-BSA- or NP32-BSA-coated plates (Vector Labs).

    Techniques: Mouse Assay, Injection, Immunofluorescence, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked immunosorbent spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay (ELISA) with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.

    Journal: The Journal of Infectious Diseases

    Article Title: Distinct Cross-reactive B-Cell Responses to Live Attenuated and Inactivated Influenza Vaccines

    doi: 10.1093/infdis/jiu190

    Figure Lengend Snippet: Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked immunosorbent spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay (ELISA) with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.

    Article Snippet: The concentrations of IgA and IgG in PPAbs were determined with the Immuno-Tek Quantitative Human IgA or IgG enzyme-linked immunosorbent assay (ELISA) kits (Zeptometrix), respectively.

    Techniques: ELISpot Assay, Recombinant, Binding Assay, Activity Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay

    Cross-reactive hemagglutinin (HA) binding activity of plasmablast-derived polyclonal antibodies (PPAbs) after immunization with 2010 inactivated influenza vaccine (IIV) and live attenuated influenza vaccine (LAIV). A pool of PPAbs was assembled by combining equal amounts of immunoglobulin G (IgG) from each PPAb sample in the IIV or LAIV groups. Each PPAb pool was serially diluted, starting at a concentration of 9 ng/mL for LAIV and 4.1 ng/mL for IIV of IgG, and analyzed with an enzyme-linked immunosorbent assay (ELISA) for binding to recombinant HAs from indicated influenza virus strains. Relative binding activity to each HA (numbers in each panel) was defined as the ratio of its area under curve (AUC) to that of the relevant 2010 vaccine strain (circle in each panel, assigned a value of 1).

    Journal: The Journal of Infectious Diseases

    Article Title: Distinct Cross-reactive B-Cell Responses to Live Attenuated and Inactivated Influenza Vaccines

    doi: 10.1093/infdis/jiu190

    Figure Lengend Snippet: Cross-reactive hemagglutinin (HA) binding activity of plasmablast-derived polyclonal antibodies (PPAbs) after immunization with 2010 inactivated influenza vaccine (IIV) and live attenuated influenza vaccine (LAIV). A pool of PPAbs was assembled by combining equal amounts of immunoglobulin G (IgG) from each PPAb sample in the IIV or LAIV groups. Each PPAb pool was serially diluted, starting at a concentration of 9 ng/mL for LAIV and 4.1 ng/mL for IIV of IgG, and analyzed with an enzyme-linked immunosorbent assay (ELISA) for binding to recombinant HAs from indicated influenza virus strains. Relative binding activity to each HA (numbers in each panel) was defined as the ratio of its area under curve (AUC) to that of the relevant 2010 vaccine strain (circle in each panel, assigned a value of 1).

    Article Snippet: The concentrations of IgA and IgG in PPAbs were determined with the Immuno-Tek Quantitative Human IgA or IgG enzyme-linked immunosorbent assay (ELISA) kits (Zeptometrix), respectively.

    Techniques: Binding Assay, Activity Assay, Derivative Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Recombinant

    In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and IgG2a levels were determined by ELISA. Data are expressed as means ± SDs. * P

    Journal: Journal of Translational Medicine

    Article Title: Fn14-Fc suppresses germinal center formation and pathogenic B cells in a lupus mouse model via inhibition of the TWEAK/Fn14 Pathway

    doi: 10.1186/s12967-016-0846-4

    Figure Lengend Snippet: In vivo effect of Fn14-Fc on Ig production in SLE mice. Sanroque mice were injected intraperitoneally with Fn14-Fc (100 μg/mouse) or control-Fc (100 μg/mouse) (n = 5/group) for 3 weeks. Mice were sacrificed on day 21 after the first injection. The serum total IgG, IgG1 and IgG2a levels were determined by ELISA. Data are expressed as means ± SDs. * P

    Article Snippet: Total IgG, IgG1, IgG2a, and anti-double-stranded (ds) DNA Abs were measured using a mouse total IgG, IgG1 and IgG2a enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl Laboratories, Montgomery, TX, USA).

    Techniques: In Vivo, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    UV-inactivated MV-NIS remains antigenic. Notes: MV-NIS was inactivated by 0.5 Joule/cm 2 UV-C light for 0.5 h. UV-inactivated MV-NIS was serially diluted in the presence of 2% measles immune serum and pre-incubated for 0.5 h at room temperature. An ELISA was performed using plates coated with MV antigens. Optical density at 450 nm (OD 450 ) was measured to determine the amount of anti-MV IgGs that were initially not caught by the decoy virus and could therefore bind to the coated MV antigens. Technical positive and negative controls were run in parallel. Abbreviations: ELISA, enzyme-linked immunosorbent assay; UV, ultraviolet; UV-C, short-wavelength ultraviolet light; MV-NIS, human sodium iodide symporter measles virus; IgG, immunoglobulin G; OD, optical density; Pos, positive; Neg, negative.

    Journal: Oncolytic Virotherapy

    Article Title: Proof-of-principle that a decoy virus protects oncolytic measles virus against neutralizing antibodies

    doi: 10.2147/OV.S150637

    Figure Lengend Snippet: UV-inactivated MV-NIS remains antigenic. Notes: MV-NIS was inactivated by 0.5 Joule/cm 2 UV-C light for 0.5 h. UV-inactivated MV-NIS was serially diluted in the presence of 2% measles immune serum and pre-incubated for 0.5 h at room temperature. An ELISA was performed using plates coated with MV antigens. Optical density at 450 nm (OD 450 ) was measured to determine the amount of anti-MV IgGs that were initially not caught by the decoy virus and could therefore bind to the coated MV antigens. Technical positive and negative controls were run in parallel. Abbreviations: ELISA, enzyme-linked immunosorbent assay; UV, ultraviolet; UV-C, short-wavelength ultraviolet light; MV-NIS, human sodium iodide symporter measles virus; IgG, immunoglobulin G; OD, optical density; Pos, positive; Neg, negative.

    Article Snippet: Anti-MV IgG enzyme-linked immunosorbent assay An anti-MV IgG enzyme-linked immunosorbent assay (ELISA) (LS-F10275; LifeSpan Biosciences, Seattle, WA, USA) was used to validate the antigenicity of UV-inactivated MV-NIS.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay