ige antibodies Search Results


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  • 94
    Thermo Fisher ige antibodies
    Figure 3. Serum Bet v 1-specific <t>IgE</t> levels and <t>IgE/IgG2a</t> ratios. ( A ) Significantly higher rBet v 1-specific IgE level are found in sensitized/not treated or treated mice compared with naïve ( P
    Ige Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore dnp specific ige ab
    <t>IgE-dependent</t> systemic PSA is markedly reduced in A1-knockdown mice. ( A ) WT mice ( n = 12), VVFF mice ( n = 8), and VVA1 mice ( n = 8) were sensitized passively by i.p. injection of 10 ng of <t>DNP-specific</t> IgE (SPE-7) and then challenged by i.p. injection
    Dnp Specific Ige Ab, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad ige antibody
    <t>IgE-dependent</t> systemic PSA is markedly reduced in A1-knockdown mice. ( A ) WT mice ( n = 12), VVFF mice ( n = 8), and VVA1 mice ( n = 8) were sensitized passively by i.p. injection of 10 ng of <t>DNP-specific</t> IgE (SPE-7) and then challenged by i.p. injection
    Ige Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    SouthernBiotech anti mouse ige ab
    Effects of <t>IgE</t> in mouse sera on mast cells. ( A ) <t>Immunoblotting</t> of IgE- or IgG-depleted sera. ( B , C ) Effects of the sera were analyzed as in . nd, non-depleted sera. ( D ) IL-6 production by sera from epicutaneously OVA-immunized mice.
    Anti Mouse Ige Ab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    SouthernBiotech ige detection ab solution
    Effects of <t>IgE</t> in mouse sera on mast cells. ( A ) <t>Immunoblotting</t> of IgE- or IgG-depleted sera. ( B , C ) Effects of the sera were analyzed as in . nd, non-depleted sera. ( D ) IL-6 production by sera from epicutaneously OVA-immunized mice.
    Ige Detection Ab Solution, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson anti mouse ige ab pairs
    Effects of <t>IgE</t> in mouse sera on mast cells. ( A ) <t>Immunoblotting</t> of IgE- or IgG-depleted sera. ( B , C ) Effects of the sera were analyzed as in . nd, non-depleted sera. ( D ) IL-6 production by sera from epicutaneously OVA-immunized mice.
    Anti Mouse Ige Ab Pairs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Miltenyi Biotec ige antibodies
    Effects of <t>IgE</t> in mouse sera on mast cells. ( A ) <t>Immunoblotting</t> of IgE- or IgG-depleted sera. ( B , C ) Effects of the sera were analyzed as in . nd, non-depleted sera. ( D ) IL-6 production by sera from epicutaneously OVA-immunized mice.
    Ige Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore dnp immunoglobulin e dnp ige
    Inhibitory effects of KIOM-MA128 on the production of allergic mediators. <t>IgE-sensitized</t> RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p
    Dnp Immunoglobulin E Dnp Ige, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bethyl anti mouse ige polyclonal ab
    Inhibitory effects of KIOM-MA128 on the production of allergic mediators. <t>IgE-sensitized</t> RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p
    Anti Mouse Ige Polyclonal Ab, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson ige antibodies
    Serum antibody response in mice after sublingual immunization with Phleum pratense pollen allergens. The immunogens were as follows: N (native allergen); PM ( PM ‐allergoids); PM ‐ OX ( PM ‐allergoids further oxidized); PBS (phosphate‐buffered saline as a negative control). (A) Serum levels of <t>IgG1,</t> IgG2a, and <t>IgE</t> were measured by ELISA against native allergens ( Phleum pratense ) in mice immunized with different protocols. (B) IgG2a/IgE ratios of antibody levels from each individual mouse immunized with different protocols. Results are the mean ± SEM of 6 mice. Statistical differences were analyzed with Student's t test. * P
    Ige Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Pharmingen ige antibodies
    Allergen-specific antibody responses and RBL cell mediator release in sensitized and tolerized mice. (a) Serum <t>IgG1</t> and <t>IgE</t> antibody levels against rHev b 1 and rHev b 3 were detected by ELISA. Open bars (sens) indicate mean OD of five mice sensitized with rHev b 1/Al(OH) 3 or rHev b 3/Al(OH) 3 . Black bars (tol Hev b 1) or grey bars (tol Hev b 3) show mean OD of five mice i.n. treated with rHev b 1 or rHev b 3 prior to sensitization. (b) Allergen-induced β-hexosaminidase release from RBL cells after incubation with sera from five Hev b 1/Hev b 3 (white bars) sensitized mice or five Hev b 1 (black bars)/Hev b 3 (grey bars) pretreated mice. Error bars indicate s.e.m. * P
    Ige Antibodies, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore ige antibodies
    Antibody detection (IgG, IgG1, IgG2a, <t>IgE</t> and IgM) against rFh15 or rFh15b 2 weeks after immunisation schedule in BALB/c mice. Data presented as the mean ± standard error of the mean. Groups: Untreated; Treated with AA0029+Qs; Immunised with AA0029 + Qs + rFh15; and Immunised with AA0029+Qs+rFh15b. O.D., optical density; * P
    Ige Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    SouthernBiotech ige antibodies
    B7h regulates T-dependent immune responses in vivo . B7h +/+ and B7h –/– mice (three per group) were immunized with KLH in CFA. Eleven days later, the mice were killed and analyzed. ( A ) Anti-KLH antibodies <t>(IgM,</t> IgG1, IgG2a, and <t>IgE)</t> were measured in the sera by ELISA. The sera from B7h +/+ and B7h –/– mice were subject to a 3-fold serial dilution, and the concentrations of KLH-specific IgM, IgG1, Ig2a, and IgE were analyzed by ELISA and averaged for each group. ( B ) Spleen cells from immunized mice were stimulated in 96-well plates as triplicates with the indicated concentration of KLH peptide. Proliferation was assayed after 3 days of treatment by adding [ 3 H]thymidine to the culture for the last 8 h. IL-2 was measured 1 day later, and effector cytokines (IFN-γ and IL-4) were measured after 4 days of treatment.
    Ige Antibodies, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Jackson Immuno ige antibodies
    B7h regulates T-dependent immune responses in vivo . B7h +/+ and B7h –/– mice (three per group) were immunized with KLH in CFA. Eleven days later, the mice were killed and analyzed. ( A ) Anti-KLH antibodies <t>(IgM,</t> IgG1, IgG2a, and <t>IgE)</t> were measured in the sera by ELISA. The sera from B7h +/+ and B7h –/– mice were subject to a 3-fold serial dilution, and the concentrations of KLH-specific IgM, IgG1, Ig2a, and IgE were analyzed by ELISA and averaged for each group. ( B ) Spleen cells from immunized mice were stimulated in 96-well plates as triplicates with the indicated concentration of KLH peptide. Proliferation was assayed after 3 days of treatment by adding [ 3 H]thymidine to the culture for the last 8 h. IL-2 was measured 1 day later, and effector cytokines (IFN-γ and IL-4) were measured after 4 days of treatment.
    Ige Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies anti ige ab
    B7h regulates T-dependent immune responses in vivo . B7h +/+ and B7h –/– mice (three per group) were immunized with KLH in CFA. Eleven days later, the mice were killed and analyzed. ( A ) Anti-KLH antibodies <t>(IgM,</t> IgG1, IgG2a, and <t>IgE)</t> were measured in the sera by ELISA. The sera from B7h +/+ and B7h –/– mice were subject to a 3-fold serial dilution, and the concentrations of KLH-specific IgM, IgG1, Ig2a, and IgE were analyzed by ELISA and averaged for each group. ( B ) Spleen cells from immunized mice were stimulated in 96-well plates as triplicates with the indicated concentration of KLH peptide. Proliferation was assayed after 3 days of treatment by adding [ 3 H]thymidine to the culture for the last 8 h. IL-2 was measured 1 day later, and effector cytokines (IFN-γ and IL-4) were measured after 4 days of treatment.
    Anti Ige Ab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Pharmingen fitc rat anti mouse ige ab
    B7h regulates T-dependent immune responses in vivo . B7h +/+ and B7h –/– mice (three per group) were immunized with KLH in CFA. Eleven days later, the mice were killed and analyzed. ( A ) Anti-KLH antibodies <t>(IgM,</t> IgG1, IgG2a, and <t>IgE)</t> were measured in the sera by ELISA. The sera from B7h +/+ and B7h –/– mice were subject to a 3-fold serial dilution, and the concentrations of KLH-specific IgM, IgG1, Ig2a, and IgE were analyzed by ELISA and averaged for each group. ( B ) Spleen cells from immunized mice were stimulated in 96-well plates as triplicates with the indicated concentration of KLH peptide. Proliferation was assayed after 3 days of treatment by adding [ 3 H]thymidine to the culture for the last 8 h. IL-2 was measured 1 day later, and effector cytokines (IFN-γ and IL-4) were measured after 4 days of treatment.
    Fitc Rat Anti Mouse Ige Ab, supplied by Pharmingen, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson ige specific antibody
    B7h regulates T-dependent immune responses in vivo . B7h +/+ and B7h –/– mice (three per group) were immunized with KLH in CFA. Eleven days later, the mice were killed and analyzed. ( A ) Anti-KLH antibodies <t>(IgM,</t> IgG1, IgG2a, and <t>IgE)</t> were measured in the sera by ELISA. The sera from B7h +/+ and B7h –/– mice were subject to a 3-fold serial dilution, and the concentrations of KLH-specific IgM, IgG1, Ig2a, and IgE were analyzed by ELISA and averaged for each group. ( B ) Spleen cells from immunized mice were stimulated in 96-well plates as triplicates with the indicated concentration of KLH peptide. Proliferation was assayed after 3 days of treatment by adding [ 3 H]thymidine to the culture for the last 8 h. IL-2 was measured 1 day later, and effector cytokines (IFN-γ and IL-4) were measured after 4 days of treatment.
    Ige Specific Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher in vitro ige detection
    For <t>ISAC</t> inhibition studies, patients ( n = 15) of three geographically distinct areas were recruited, a schema of seasonal Fagales pollen counts is provided for each area (a). Corylaceae include the genera Corylus , Carpinus and Ostrya ]. Serum specific <t>IgE-binding</t> inhibition was performed with either Bet v 1.0101 or Ost c 1.0101 for the respective sera (b, c and d) and is presented as % values of total inhibition. Immobilized Betuloideae allergens are indicated in dark grey, Coryloideae allergens in grey and Fagaceae allergens in light grey.
    In Vitro Ige Detection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Figure 3. Serum Bet v 1-specific IgE levels and IgE/IgG2a ratios. ( A ) Significantly higher rBet v 1-specific IgE level are found in sensitized/not treated or treated mice compared with naïve ( P

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: rBet v 1 immunotherapy of sensitized mice with Streptococcus thermophilus as vehicle and adjuvant

    doi: 10.4161/hv.28155

    Figure Lengend Snippet: Figure 3. Serum Bet v 1-specific IgE levels and IgE/IgG2a ratios. ( A ) Significantly higher rBet v 1-specific IgE level are found in sensitized/not treated or treated mice compared with naïve ( P

    Article Snippet: After washing, anti-mouse IgG2a and IgE antibodies (1 μg⋅mL−1 , Pierce antibodies) were applied overnight at 4 °C.

    Techniques: Mouse Assay

    IgE-dependent systemic PSA is markedly reduced in A1-knockdown mice. ( A ) WT mice ( n = 12), VVFF mice ( n = 8), and VVA1 mice ( n = 8) were sensitized passively by i.p. injection of 10 ng of DNP-specific IgE (SPE-7) and then challenged by i.p. injection

    Journal: The Journal of Immunology Author Choice

    Article Title: Knockdown of the Antiapoptotic Bcl-2 Family Member A1/Bfl-1 Protects Mice from Anaphylaxis

    doi: 10.4049/jimmunol.1400637

    Figure Lengend Snippet: IgE-dependent systemic PSA is markedly reduced in A1-knockdown mice. ( A ) WT mice ( n = 12), VVFF mice ( n = 8), and VVA1 mice ( n = 8) were sensitized passively by i.p. injection of 10 ng of DNP-specific IgE (SPE-7) and then challenged by i.p. injection

    Article Snippet: Briefly, mice under anesthesia and analgesia were sensitized passively with IgE by intradermal injection of 20 ng DNP-specific IgE Ab (clone SPE-7; Sigma-Aldrich) in 20 μl PBS and were challenged 24 h later by i.v. injection with 200 μg DNP (Sigma-Aldrich) in 100 μl PBS.

    Techniques: Mouse Assay, Injection

    A1-knockdown mice exhibit markedly reduced IgE-dependent cutaneous PCA. ( A ) WT mice ( n = 6), VVFF mice ( n = 7), and VVA1 mice ( n = 5) were sensitized passively by intradermal injection of 20 ng of DNP-specific IgE (SPE-7) and then challenged by i.v. injection

    Journal: The Journal of Immunology Author Choice

    Article Title: Knockdown of the Antiapoptotic Bcl-2 Family Member A1/Bfl-1 Protects Mice from Anaphylaxis

    doi: 10.4049/jimmunol.1400637

    Figure Lengend Snippet: A1-knockdown mice exhibit markedly reduced IgE-dependent cutaneous PCA. ( A ) WT mice ( n = 6), VVFF mice ( n = 7), and VVA1 mice ( n = 5) were sensitized passively by intradermal injection of 20 ng of DNP-specific IgE (SPE-7) and then challenged by i.v. injection

    Article Snippet: Briefly, mice under anesthesia and analgesia were sensitized passively with IgE by intradermal injection of 20 ng DNP-specific IgE Ab (clone SPE-7; Sigma-Aldrich) in 20 μl PBS and were challenged 24 h later by i.v. injection with 200 μg DNP (Sigma-Aldrich) in 100 μl PBS.

    Techniques: Mouse Assay, Injection

    Effects of IgE in mouse sera on mast cells. ( A ) Immunoblotting of IgE- or IgG-depleted sera. ( B , C ) Effects of the sera were analyzed as in . nd, non-depleted sera. ( D ) IL-6 production by sera from epicutaneously OVA-immunized mice.

    Journal: Allergology international : official journal of the Japanese Society of Allergology

    Article Title: Polyclonal IgE Induces Mast Cell Survival and Cytokine Production

    doi: 10.2332/allergolint.08-OA-0080

    Figure Lengend Snippet: Effects of IgE in mouse sera on mast cells. ( A ) Immunoblotting of IgE- or IgG-depleted sera. ( B , C ) Effects of the sera were analyzed as in . nd, non-depleted sera. ( D ) IL-6 production by sera from epicutaneously OVA-immunized mice.

    Article Snippet: Cleared sera were analyzed by SDS-PAGE followed by immunoblotting with anti-mouse IgG Ab (Amersham), anti-mouse IgE Ab (SouthernBiotech, Birmingham, AL, USA) or anti-human IgE Ab (EMD Biosciences Inc., La Jolla, CA, USA).

    Techniques: Mouse Assay

    Effects of purified polyclonal IgE on BMMCs. ( A ) Chromatogram of gel filtration during IgE purification. The fraction used for subsequent experiments (indicated by a horizontal line) was analyzed by non-reducing SDS-PAGE and Commassie blue staining (

    Journal: Allergology international : official journal of the Japanese Society of Allergology

    Article Title: Polyclonal IgE Induces Mast Cell Survival and Cytokine Production

    doi: 10.2332/allergolint.08-OA-0080

    Figure Lengend Snippet: Effects of purified polyclonal IgE on BMMCs. ( A ) Chromatogram of gel filtration during IgE purification. The fraction used for subsequent experiments (indicated by a horizontal line) was analyzed by non-reducing SDS-PAGE and Commassie blue staining (

    Article Snippet: Cleared sera were analyzed by SDS-PAGE followed by immunoblotting with anti-mouse IgG Ab (Amersham), anti-mouse IgE Ab (SouthernBiotech, Birmingham, AL, USA) or anti-human IgE Ab (EMD Biosciences Inc., La Jolla, CA, USA).

    Techniques: Purification, Filtration, SDS Page, Staining

    Inhibitory effects of KIOM-MA128 on the production of allergic mediators. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on the production of allergic mediators. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques:

    Inhibitory effects of KIOM-MA128 on PGD 2 production and the activation of the arachidonate cascade. RBL-2H3 mast cells were seeded on a 6-well plate (5 × 10 5 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. The amounts of PGD 2 were determined as described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. The cells were washed with 1× DPBS and lysed with cell lysis buffer. The levels of p-cPLA 2 , COX-2 and α-tubulin were determined using the procedure described in the Materials and Methods section. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on PGD 2 production and the activation of the arachidonate cascade. RBL-2H3 mast cells were seeded on a 6-well plate (5 × 10 5 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. The amounts of PGD 2 were determined as described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. The cells were washed with 1× DPBS and lysed with cell lysis buffer. The levels of p-cPLA 2 , COX-2 and α-tubulin were determined using the procedure described in the Materials and Methods section. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Activation Assay, Incubation, Lysis

    Inhibitory effect of KIOM-MA128 on Ag/IgE-induced passive cutaneous anaphylaxis in mice. IgE-sensitized mice were orally administered KIOM-MA128 (0–100 mg/kg) for 1 h and then intravenously injected with 100 μg DNP-HSA containing 0.5% Evans blue. After 30 min, the mice were euthanized, and then both ears were excised. The extravasated dye in the ears was analyzed using the procedure described in the Materials and Methods section. The data are listed as the mean ± SEM values from eight determinations. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effect of KIOM-MA128 on Ag/IgE-induced passive cutaneous anaphylaxis in mice. IgE-sensitized mice were orally administered KIOM-MA128 (0–100 mg/kg) for 1 h and then intravenously injected with 100 μg DNP-HSA containing 0.5% Evans blue. After 30 min, the mice were euthanized, and then both ears were excised. The extravasated dye in the ears was analyzed using the procedure described in the Materials and Methods section. The data are listed as the mean ± SEM values from eight determinations. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Mouse Assay, Injection

    Inhibitory effects of KIOM-MA128 on the activation of the FcεRI cascade. IgE-sensitized RBL-2H3 cells were treated with KIOM-MA128 for 1 h and then stimulated with DNP-Ag for 10 min. The cells were washed with 1 × DPBS and lysed with cell lysis buffer. The levels of p-SYK, p-LYN, p-FYN, p-ERK, p-p38, p-JNK, p-PLCγ1, p-PKCδ and α-tubulin were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. ( A ) p-SYK, p-LYN and p-FYN; ( B ) p-ERK, p-p38 and p-JNK; ( C ) p-PLCγ1 and p-PKCδ. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on the activation of the FcεRI cascade. IgE-sensitized RBL-2H3 cells were treated with KIOM-MA128 for 1 h and then stimulated with DNP-Ag for 10 min. The cells were washed with 1 × DPBS and lysed with cell lysis buffer. The levels of p-SYK, p-LYN, p-FYN, p-ERK, p-p38, p-JNK, p-PLCγ1, p-PKCδ and α-tubulin were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. ( A ) p-SYK, p-LYN and p-FYN; ( B ) p-ERK, p-p38 and p-JNK; ( C ) p-PLCγ1 and p-PKCδ. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Activation Assay, Lysis

    Inhibitory effects of KIOM-MA128 on degranulation and histamine release. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h before antigen challenge. β-hexosaminidase activity and the histamine concentrations were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on degranulation and histamine release. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h before antigen challenge. β-hexosaminidase activity and the histamine concentrations were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Activity Assay

    Effect of KIOM-MA128 on cell viability in IgE/Ag-activated RBL-2H3 mast cells. RBL-2H3 mast cells were seeded on a 96-well plate (1 × 10 4 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. Cell viability was determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments.

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Effect of KIOM-MA128 on cell viability in IgE/Ag-activated RBL-2H3 mast cells. RBL-2H3 mast cells were seeded on a 96-well plate (1 × 10 4 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. Cell viability was determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments.

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Incubation

    Serum antibody response in mice after sublingual immunization with Phleum pratense pollen allergens. The immunogens were as follows: N (native allergen); PM ( PM ‐allergoids); PM ‐ OX ( PM ‐allergoids further oxidized); PBS (phosphate‐buffered saline as a negative control). (A) Serum levels of IgG1, IgG2a, and IgE were measured by ELISA against native allergens ( Phleum pratense ) in mice immunized with different protocols. (B) IgG2a/IgE ratios of antibody levels from each individual mouse immunized with different protocols. Results are the mean ± SEM of 6 mice. Statistical differences were analyzed with Student's t test. * P

    Journal: Allergy

    Article Title: Oral myeloid cells uptake allergoids coupled to mannan driving Th1/Treg responses upon sublingual delivery in mice, et al. Oral myeloid cells uptake allergoids coupled to mannan driving Th1/Treg responses upon sublingual delivery in mice

    doi: 10.1111/all.13396

    Figure Lengend Snippet: Serum antibody response in mice after sublingual immunization with Phleum pratense pollen allergens. The immunogens were as follows: N (native allergen); PM ( PM ‐allergoids); PM ‐ OX ( PM ‐allergoids further oxidized); PBS (phosphate‐buffered saline as a negative control). (A) Serum levels of IgG1, IgG2a, and IgE were measured by ELISA against native allergens ( Phleum pratense ) in mice immunized with different protocols. (B) IgG2a/IgE ratios of antibody levels from each individual mouse immunized with different protocols. Results are the mean ± SEM of 6 mice. Statistical differences were analyzed with Student's t test. * P

    Article Snippet: IgG1, IgG2a, and IgE antibodies were determined using appropriate anti‐mouse isotype labeled antibodies (BD Pharmingen).

    Techniques: Mouse Assay, Negative Control, Enzyme-linked Immunosorbent Assay

    Allergen-specific antibody responses and RBL cell mediator release in sensitized and tolerized mice. (a) Serum IgG1 and IgE antibody levels against rHev b 1 and rHev b 3 were detected by ELISA. Open bars (sens) indicate mean OD of five mice sensitized with rHev b 1/Al(OH) 3 or rHev b 3/Al(OH) 3 . Black bars (tol Hev b 1) or grey bars (tol Hev b 3) show mean OD of five mice i.n. treated with rHev b 1 or rHev b 3 prior to sensitization. (b) Allergen-induced β-hexosaminidase release from RBL cells after incubation with sera from five Hev b 1/Hev b 3 (white bars) sensitized mice or five Hev b 1 (black bars)/Hev b 3 (grey bars) pretreated mice. Error bars indicate s.e.m. * P

    Journal: Clinical and Experimental Immunology

    Article Title: Induction of mucosal tolerance with recombinant Hev b 1 and recombinant Hev b 3 for prevention of latex allergy in BALB/c mice

    doi: 10.1046/j.1365-2249.2003.02210.x

    Figure Lengend Snippet: Allergen-specific antibody responses and RBL cell mediator release in sensitized and tolerized mice. (a) Serum IgG1 and IgE antibody levels against rHev b 1 and rHev b 3 were detected by ELISA. Open bars (sens) indicate mean OD of five mice sensitized with rHev b 1/Al(OH) 3 or rHev b 3/Al(OH) 3 . Black bars (tol Hev b 1) or grey bars (tol Hev b 3) show mean OD of five mice i.n. treated with rHev b 1 or rHev b 3 prior to sensitization. (b) Allergen-induced β-hexosaminidase release from RBL cells after incubation with sera from five Hev b 1/Hev b 3 (white bars) sensitized mice or five Hev b 1 (black bars)/Hev b 3 (grey bars) pretreated mice. Error bars indicate s.e.m. * P

    Article Snippet: Rat antimouse IgG1, IgG2a and IgE antibodies (1/500, Pharmingen, San Diego, CA, USA) followed by peroxidase-conjugated mouse antirat IgG antibodies (1/2000, Jackson Immuno Laboratory, West Grove, PA, USA) were used.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Incubation

    Antibody detection (IgG, IgG1, IgG2a, IgE and IgM) against rFh15 or rFh15b 2 weeks after immunisation schedule in BALB/c mice. Data presented as the mean ± standard error of the mean. Groups: Untreated; Treated with AA0029+Qs; Immunised with AA0029 + Qs + rFh15; and Immunised with AA0029+Qs+rFh15b. O.D., optical density; * P

    Journal: Parasites & Vectors

    Article Title: Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems

    doi: 10.1186/s13071-016-1500-y

    Figure Lengend Snippet: Antibody detection (IgG, IgG1, IgG2a, IgE and IgM) against rFh15 or rFh15b 2 weeks after immunisation schedule in BALB/c mice. Data presented as the mean ± standard error of the mean. Groups: Untreated; Treated with AA0029+Qs; Immunised with AA0029 + Qs + rFh15; and Immunised with AA0029+Qs+rFh15b. O.D., optical density; * P

    Article Snippet: Sera were then added at 1:100 dilutions and incubated for 1 h at 37 °C, followed by the addition of goat peroxidase-labelled anti-mouse IgG, IgG1, IgG2a, IgM or IgE antibodies at 1:1000 dilution (Sigma, St. Louis, MO, USA).

    Techniques: Mouse Assay

    B7h regulates T-dependent immune responses in vivo . B7h +/+ and B7h –/– mice (three per group) were immunized with KLH in CFA. Eleven days later, the mice were killed and analyzed. ( A ) Anti-KLH antibodies (IgM, IgG1, IgG2a, and IgE) were measured in the sera by ELISA. The sera from B7h +/+ and B7h –/– mice were subject to a 3-fold serial dilution, and the concentrations of KLH-specific IgM, IgG1, Ig2a, and IgE were analyzed by ELISA and averaged for each group. ( B ) Spleen cells from immunized mice were stimulated in 96-well plates as triplicates with the indicated concentration of KLH peptide. Proliferation was assayed after 3 days of treatment by adding [ 3 H]thymidine to the culture for the last 8 h. IL-2 was measured 1 day later, and effector cytokines (IFN-γ and IL-4) were measured after 4 days of treatment.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: B7h is required for T cell activation, differentiation, and effector function

    doi: 10.1073/pnas.2335041100

    Figure Lengend Snippet: B7h regulates T-dependent immune responses in vivo . B7h +/+ and B7h –/– mice (three per group) were immunized with KLH in CFA. Eleven days later, the mice were killed and analyzed. ( A ) Anti-KLH antibodies (IgM, IgG1, IgG2a, and IgE) were measured in the sera by ELISA. The sera from B7h +/+ and B7h –/– mice were subject to a 3-fold serial dilution, and the concentrations of KLH-specific IgM, IgG1, Ig2a, and IgE were analyzed by ELISA and averaged for each group. ( B ) Spleen cells from immunized mice were stimulated in 96-well plates as triplicates with the indicated concentration of KLH peptide. Proliferation was assayed after 3 days of treatment by adding [ 3 H]thymidine to the culture for the last 8 h. IL-2 was measured 1 day later, and effector cytokines (IFN-γ and IL-4) were measured after 4 days of treatment.

    Article Snippet: KLH-specific antibodies were detected with biotinylated goat antimouse IgM, rat anti-mouse IgG1, IgG2a, and IgE antibodies (Southern Biotechnology Associates).

    Techniques: In Vivo, Mouse Assay, Enzyme-linked Immunosorbent Assay, Serial Dilution, Concentration Assay

    For ISAC inhibition studies, patients ( n = 15) of three geographically distinct areas were recruited, a schema of seasonal Fagales pollen counts is provided for each area (a). Corylaceae include the genera Corylus , Carpinus and Ostrya ]. Serum specific IgE-binding inhibition was performed with either Bet v 1.0101 or Ost c 1.0101 for the respective sera (b, c and d) and is presented as % values of total inhibition. Immobilized Betuloideae allergens are indicated in dark grey, Coryloideae allergens in grey and Fagaceae allergens in light grey.

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    Article Title: Bet v 1-like pollen allergens of multiple Fagales species can sensitize atopic individuals

    doi: 10.1111/j.1365-2222.2011.03866.x

    Figure Lengend Snippet: For ISAC inhibition studies, patients ( n = 15) of three geographically distinct areas were recruited, a schema of seasonal Fagales pollen counts is provided for each area (a). Corylaceae include the genera Corylus , Carpinus and Ostrya ]. Serum specific IgE-binding inhibition was performed with either Bet v 1.0101 or Ost c 1.0101 for the respective sera (b, c and d) and is presented as % values of total inhibition. Immobilized Betuloideae allergens are indicated in dark grey, Coryloideae allergens in grey and Fagaceae allergens in light grey.

    Article Snippet: Fagales pollen allergic patients were selected based on case history, positive skin prick test and in vitro IgE detection using Immuno Solid-phase Allergen Chip (ISAC) 103 (Phadia Multiplexing Diagnostics GMBH, Vienna, Austria) [ ] according to previously reported protocols [ ].

    Techniques: Inhibition, Binding Assay