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  • 94
    Thermo Fisher iga mouse uncoated elisa kit
    The reduction of IgD + and IgM + B cells of colon in IMQ mice was not caused by migration to skin or skin draining lymph nodes, or by systemic B-cell depletion. ( A ) Representative plots of flow cytometry analysis for lymphocytes in CD45 + colonic lamina propria. ( B and C ) Percentage ( left ) and absolute number ( right ) of ( B ) T cells and ( C ) B cells in CD45 + colonic lamina propria as shown in panel A . ( D ) Representative plots of flow cytometry analysis for IFN-γ and IL17A in CD3 + CD4 + cells from colonic lamina propria, skin dLN. and mesenteric lymph node (MLN), stimulated with PMA, ionomycin, and IL23 for 4 hours. ( E ) Percentage of IFN-γ + ( top ) and IL17A + ( bottom ) cells in CD3 + CD4 + lymphocytes from each organ. ( F and G ) Intracellular staining and the ratio of Foxp3 in CD3 + CD4 + lymphocytes from each organ. ( H ) Absolute number of <t>IgA</t> + B cells ( left ), IgD + B cells ( middle ), and IgM + B cells ( right ). ( I ) Percentage in CD45 + cells of IgA + B220 - plasma cells. ( J ) Concentration of IgA in feces measured by enzyme-linked immunosorbent assay. ( K ) Percentage of IgA-coated bacteria in feces. ( L ) Representative plots of flow cytometry analysis for B cells in epidermis, dermis, skin dLNs, spleen, and MLN after topical IMQ treatment. The numbers within plots indicate the percentages of gated cells. The plots are gated on CD45 + cells. ( M ) Percentages of B cells in CD45 + cells in epidermis, dermis, skin dLN, spleen, and MLN as shown in panel L . ( N ) Absolute number of B cells ( top ), IgD + B cells ( middle ), and IgM + B cells ( bottom ) in skin dLN and MLN. Each symbol represents an individual mouse (n = 4–8). Statistical analyses were performed with the Student t test. * P
    Iga Mouse Uncoated Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EUROIMMUN iga elisa
    The reduction of IgD + and IgM + B cells of colon in IMQ mice was not caused by migration to skin or skin draining lymph nodes, or by systemic B-cell depletion. ( A ) Representative plots of flow cytometry analysis for lymphocytes in CD45 + colonic lamina propria. ( B and C ) Percentage ( left ) and absolute number ( right ) of ( B ) T cells and ( C ) B cells in CD45 + colonic lamina propria as shown in panel A . ( D ) Representative plots of flow cytometry analysis for IFN-γ and IL17A in CD3 + CD4 + cells from colonic lamina propria, skin dLN. and mesenteric lymph node (MLN), stimulated with PMA, ionomycin, and IL23 for 4 hours. ( E ) Percentage of IFN-γ + ( top ) and IL17A + ( bottom ) cells in CD3 + CD4 + lymphocytes from each organ. ( F and G ) Intracellular staining and the ratio of Foxp3 in CD3 + CD4 + lymphocytes from each organ. ( H ) Absolute number of <t>IgA</t> + B cells ( left ), IgD + B cells ( middle ), and IgM + B cells ( right ). ( I ) Percentage in CD45 + cells of IgA + B220 - plasma cells. ( J ) Concentration of IgA in feces measured by enzyme-linked immunosorbent assay. ( K ) Percentage of IgA-coated bacteria in feces. ( L ) Representative plots of flow cytometry analysis for B cells in epidermis, dermis, skin dLNs, spleen, and MLN after topical IMQ treatment. The numbers within plots indicate the percentages of gated cells. The plots are gated on CD45 + cells. ( M ) Percentages of B cells in CD45 + cells in epidermis, dermis, skin dLN, spleen, and MLN as shown in panel L . ( N ) Absolute number of B cells ( top ), IgD + B cells ( middle ), and IgM + B cells ( bottom ) in skin dLN and MLN. Each symbol represents an individual mouse (n = 4–8). Statistical analyses were performed with the Student t test. * P
    Iga Elisa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher mouse iga elisa kit
    Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial <t>IgA</t> and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by <t>ELISA.</t> Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p
    Mouse Iga Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam chicken iga elisa kit
    Comparative titres of mucosal <t>IgA.</t> Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific <t>ELISA.</t> Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P
    Chicken Iga Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Virion\Serion elisa classic adenovirus iga kit
    Comparative titres of mucosal <t>IgA.</t> Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific <t>ELISA.</t> Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P
    Elisa Classic Adenovirus Iga Kit, supplied by Virion\Serion, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Euro Diagnostica anti ttg iga elisa
    Comparative titres of mucosal <t>IgA.</t> Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific <t>ELISA.</t> Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P
    Anti Ttg Iga Elisa, supplied by Euro Diagnostica, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam human iga elisa kit
    <t>IgA-Binding</t> of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and <t>ELISA.</t> (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p
    Human Iga Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher iga specific elisa kit
    Bacteriophages act to induce both commensal and phage specific immunity. (A) IFN-γ measured by <t>ELISA</t> in the supernatant of BMDCs from MHCII −/− mice pulsed with phage for 24 hours and co-cultured with WT T cells for 72 hours. (B) IFN-γ measured by ELISA in the supernatant of BMDCs from WT mice pulsed with antigens and ovalbumin (100 μg/mL) and co-cultured with CD4+ T cells from OTII mice. (C, D) Experimental set-up of Phage-Ovalbumin co-culture experiments. IFN-γ was measured in the supernatant by ELISA. (E) Feces from bacteriophage cocktail treated germfree mice assayed for phage specific <t>IgA</t> by ELISA. Data are represented as mean ± SEM. *p
    Iga Specific Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher mouse iga elisa ready set go
    <t>IgA</t> increases at multiple mucosal sites after recolonization of GF SW mice with Bacteroides acidifaciens. GF SW mice (n = 5–7) were orally gavaged with (1 × 10 8 CFU/mL) B. acidifaciens and kept in GF housing for 21 days. (A) Significance of changes in the colon IgA gene transcripts over time was determined by 1-way ANOVA followed by Dunn's comparison test. (B) Stool samples were assayed for SIgA by <t>ELISA.</t> Significance of changes in gut SIgA over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (C) Changes in IgA transcript levels in the small-intestine samples. Significance was determined by Student's t-test. (D) Significance of changes in LG IgA transcripts over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (E) Pooled eyewash samples were collected from mice and analyzed for IgA by ELISA. Significance of changes in eyewash SIgA over time was determined by 1-way ANOVA followed by Dunn's comparison test. Cumulatively, the data show that gut reconstitution of GF SW mice with B. acidifaciens induced a robust gut and ocular IgA transcription.
    Mouse Iga Elisa Ready Set Go, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Inova Diagnostics quanta lite rh ttg iga elisa
    <t>IgA</t> increases at multiple mucosal sites after recolonization of GF SW mice with Bacteroides acidifaciens. GF SW mice (n = 5–7) were orally gavaged with (1 × 10 8 CFU/mL) B. acidifaciens and kept in GF housing for 21 days. (A) Significance of changes in the colon IgA gene transcripts over time was determined by 1-way ANOVA followed by Dunn's comparison test. (B) Stool samples were assayed for SIgA by <t>ELISA.</t> Significance of changes in gut SIgA over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (C) Changes in IgA transcript levels in the small-intestine samples. Significance was determined by Student's t-test. (D) Significance of changes in LG IgA transcripts over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (E) Pooled eyewash samples were collected from mice and analyzed for IgA by ELISA. Significance of changes in eyewash SIgA over time was determined by 1-way ANOVA followed by Dunn's comparison test. Cumulatively, the data show that gut reconstitution of GF SW mice with B. acidifaciens induced a robust gut and ocular IgA transcription.
    Quanta Lite Rh Ttg Iga Elisa, supplied by Inova Diagnostics, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    EUROIMMUN anti hev iga elisa
    <t>IgA</t> increases at multiple mucosal sites after recolonization of GF SW mice with Bacteroides acidifaciens. GF SW mice (n = 5–7) were orally gavaged with (1 × 10 8 CFU/mL) B. acidifaciens and kept in GF housing for 21 days. (A) Significance of changes in the colon IgA gene transcripts over time was determined by 1-way ANOVA followed by Dunn's comparison test. (B) Stool samples were assayed for SIgA by <t>ELISA.</t> Significance of changes in gut SIgA over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (C) Changes in IgA transcript levels in the small-intestine samples. Significance was determined by Student's t-test. (D) Significance of changes in LG IgA transcripts over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (E) Pooled eyewash samples were collected from mice and analyzed for IgA by ELISA. Significance of changes in eyewash SIgA over time was determined by 1-way ANOVA followed by Dunn's comparison test. Cumulatively, the data show that gut reconstitution of GF SW mice with B. acidifaciens induced a robust gut and ocular IgA transcription.
    Anti Hev Iga Elisa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher anti tissue transglutaminase iga elisa kit
    The flow of participants through each stage of this randomised trial. P, Prednisolone tTg: tissue <t>transglutaminase;</t> <t>ELISA:</t> enzyme-linked immunosorbent assay; n: number; ESPGHAN: European Society for Paediatric Gastroenterology, Hepatology and Nutrition; GFD: gluten-free diet; GFD+P: gluten-free diet, plus prednisolone
    Anti Tissue Transglutaminase Iga Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Eagle Biosciences human secretory iga elisa assay kit
    The flow of participants through each stage of this randomised trial. P, Prednisolone tTg: tissue <t>transglutaminase;</t> <t>ELISA:</t> enzyme-linked immunosorbent assay; n: number; ESPGHAN: European Society for Paediatric Gastroenterology, Hepatology and Nutrition; GFD: gluten-free diet; GFD+P: gluten-free diet, plus prednisolone
    Human Secretory Iga Elisa Assay Kit, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher iga elisa ready set go
    CXCR5 + CD8 + T Cells provide help to B cells for the production of immunoglobulins. Physical contact between CD8 + T Cells (red) and B cells (green) in tonsil sections, and scale bars, 50μm. (A , n = 5). Sorted tonsil B cells and sorted CD8 + T Cells at the ratio of 1:1, 1:5, and 1:10 were co-cultured with or without α-CD3/CD28 dynabeads for 10 days (B,C) . Sorted B cells were co-cultured with fresh CD8 + or fixed CD8 + T Cells, fresh CD4 + or fixed CD4 + T Cells at the ratio of 5:1 in the presence of α-CD3/CD28 dynabeads for 10 days (D) . Sorted B cells and CD8 + , CXCR5 + CD8 + , CXCR5 − CD8 + T Cells at the ratio of 5:1 were co-cultured with or without anti-IL-21 and anti-CD40L in the presence of α-CD3/CD28 dynabeads for 10 days (E) . The supernatants from the different co-cultures were analyzed by <t>ELISA</t> for the production of IgG, IgM, and <t>IgA.</t> Data are expressed as the mean ± SD, and compared with Mann–Whitney test. * P
    Iga Elisa Ready Set Go, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam iga mouse elisa kit
    CXCR5 + CD8 + T Cells provide help to B cells for the production of immunoglobulins. Physical contact between CD8 + T Cells (red) and B cells (green) in tonsil sections, and scale bars, 50μm. (A , n = 5). Sorted tonsil B cells and sorted CD8 + T Cells at the ratio of 1:1, 1:5, and 1:10 were co-cultured with or without α-CD3/CD28 dynabeads for 10 days (B,C) . Sorted B cells were co-cultured with fresh CD8 + or fixed CD8 + T Cells, fresh CD4 + or fixed CD4 + T Cells at the ratio of 5:1 in the presence of α-CD3/CD28 dynabeads for 10 days (D) . Sorted B cells and CD8 + , CXCR5 + CD8 + , CXCR5 − CD8 + T Cells at the ratio of 5:1 were co-cultured with or without anti-IL-21 and anti-CD40L in the presence of α-CD3/CD28 dynabeads for 10 days (E) . The supernatants from the different co-cultures were analyzed by <t>ELISA</t> for the production of IgG, IgM, and <t>IgA.</t> Data are expressed as the mean ± SD, and compared with Mann–Whitney test. * P
    Iga Mouse Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher iga
    The representative figure showed that SO 2 inhalation did not affect <t>IgG</t> levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : <t>IgA</t> level in the serum; f : IgA level in the nasal septum
    Iga, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EUROIMMUN igg iga elisa
    The representative figure showed that SO 2 inhalation did not affect <t>IgG</t> levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : <t>IgA</t> level in the serum; f : IgA level in the nasal septum
    Igg Iga Elisa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Crystal Chem Inc mouse iga elisa kit
    The representative figure showed that SO 2 inhalation did not affect <t>IgG</t> levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : <t>IgA</t> level in the serum; f : IgA level in the nasal septum
    Mouse Iga Elisa Kit, supplied by Crystal Chem Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The reduction of IgD + and IgM + B cells of colon in IMQ mice was not caused by migration to skin or skin draining lymph nodes, or by systemic B-cell depletion. ( A ) Representative plots of flow cytometry analysis for lymphocytes in CD45 + colonic lamina propria. ( B and C ) Percentage ( left ) and absolute number ( right ) of ( B ) T cells and ( C ) B cells in CD45 + colonic lamina propria as shown in panel A . ( D ) Representative plots of flow cytometry analysis for IFN-γ and IL17A in CD3 + CD4 + cells from colonic lamina propria, skin dLN. and mesenteric lymph node (MLN), stimulated with PMA, ionomycin, and IL23 for 4 hours. ( E ) Percentage of IFN-γ + ( top ) and IL17A + ( bottom ) cells in CD3 + CD4 + lymphocytes from each organ. ( F and G ) Intracellular staining and the ratio of Foxp3 in CD3 + CD4 + lymphocytes from each organ. ( H ) Absolute number of IgA + B cells ( left ), IgD + B cells ( middle ), and IgM + B cells ( right ). ( I ) Percentage in CD45 + cells of IgA + B220 - plasma cells. ( J ) Concentration of IgA in feces measured by enzyme-linked immunosorbent assay. ( K ) Percentage of IgA-coated bacteria in feces. ( L ) Representative plots of flow cytometry analysis for B cells in epidermis, dermis, skin dLNs, spleen, and MLN after topical IMQ treatment. The numbers within plots indicate the percentages of gated cells. The plots are gated on CD45 + cells. ( M ) Percentages of B cells in CD45 + cells in epidermis, dermis, skin dLN, spleen, and MLN as shown in panel L . ( N ) Absolute number of B cells ( top ), IgD + B cells ( middle ), and IgM + B cells ( bottom ) in skin dLN and MLN. Each symbol represents an individual mouse (n = 4–8). Statistical analyses were performed with the Student t test. * P

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Toll-Like Receptor 7 Agonist–Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells

    doi: 10.1016/j.jcmgh.2018.09.010

    Figure Lengend Snippet: The reduction of IgD + and IgM + B cells of colon in IMQ mice was not caused by migration to skin or skin draining lymph nodes, or by systemic B-cell depletion. ( A ) Representative plots of flow cytometry analysis for lymphocytes in CD45 + colonic lamina propria. ( B and C ) Percentage ( left ) and absolute number ( right ) of ( B ) T cells and ( C ) B cells in CD45 + colonic lamina propria as shown in panel A . ( D ) Representative plots of flow cytometry analysis for IFN-γ and IL17A in CD3 + CD4 + cells from colonic lamina propria, skin dLN. and mesenteric lymph node (MLN), stimulated with PMA, ionomycin, and IL23 for 4 hours. ( E ) Percentage of IFN-γ + ( top ) and IL17A + ( bottom ) cells in CD3 + CD4 + lymphocytes from each organ. ( F and G ) Intracellular staining and the ratio of Foxp3 in CD3 + CD4 + lymphocytes from each organ. ( H ) Absolute number of IgA + B cells ( left ), IgD + B cells ( middle ), and IgM + B cells ( right ). ( I ) Percentage in CD45 + cells of IgA + B220 - plasma cells. ( J ) Concentration of IgA in feces measured by enzyme-linked immunosorbent assay. ( K ) Percentage of IgA-coated bacteria in feces. ( L ) Representative plots of flow cytometry analysis for B cells in epidermis, dermis, skin dLNs, spleen, and MLN after topical IMQ treatment. The numbers within plots indicate the percentages of gated cells. The plots are gated on CD45 + cells. ( M ) Percentages of B cells in CD45 + cells in epidermis, dermis, skin dLN, spleen, and MLN as shown in panel L . ( N ) Absolute number of B cells ( top ), IgD + B cells ( middle ), and IgM + B cells ( bottom ) in skin dLN and MLN. Each symbol represents an individual mouse (n = 4–8). Statistical analyses were performed with the Student t test. * P

    Article Snippet: The supernatant was collected and the IgA concentration was measured by IgA Mouse Enzyme-Linked Immunosorbent Assay kit (88-50450-88, lot 146856016; Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Migration, Flow Cytometry, Cytometry, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial IgA and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by ELISA. Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p

    Journal: Journal of diabetes and its complications

    Article Title: Endothelial Cell-Specific Overexpression of Endothelial Nitric Oxide Synthase in Ins2Akita Mice Exacerbates Diabetic Nephropathy

    doi: 10.1016/j.jdiacomp.2018.10.003

    Figure Lengend Snippet: Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial IgA and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by ELISA. Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p

    Article Snippet: IgA levels were determined using a mouse IgA ELISA kit purchased from Thermoscientific (Frederick, MD).

    Techniques: Immunofluorescence, Mouse Assay, Staining, Electron Microscopy, Enzyme-linked Immunosorbent Assay

    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse IgA or anti-IgM antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p

    Journal: Scientific Reports

    Article Title: Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance

    doi: 10.1038/s41598-017-05212-4

    Figure Lengend Snippet: Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse IgA or anti-IgM antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p

    Article Snippet: ELISA ELISA kits for mouse IgA and IgM (eBioscience, Frankfurt am Main, Germany), mouse IL-6 and TNF-α (BioLegend, London, United Kingdom), mouse C5a (R & D Systems, Minneapolis, MN) and mouse albumin (Bethyl Laboratories Inc., Montogomery, TX) were used according to manufacturer’s instructions.

    Techniques: Binding Assay, Incubation, Mouse Assay, Staining, Flow Cytometry, Cytometry, FACS, Fluorescence

    Increased mucosal transport of secretory antibodies in inflamed lungs. ( a ) anti-pIgR and anti-Gapdh immunoblot of 20 µg protein from whole lung homogenates of SPC-HA (n = 3) and SPC-HAxTCR-HA (n = 3) mice. Densitometric quantification of protein bands is stated in arbitrary units above each lane. Relative pIgR quantity was calculated normalizing densitometric pIgR value to the corresponding Gapdh value and subsequently comparing normalized pIgR values of the SPC-HAxTCR-HA group to the SPC-HA group. Data are representative for at least two individual experiments with similar results. ( b ) Lung tissue sections were stained with anti-pIgR (green), representative alveolar structures from n = 3/group are depicted. White circles illustrate representative densitometrically quantified tissue areas. Calculated total cell fluorescence (CTCF) was determined as: Integrated density of fluorescence-positive cell – (Area of fluorescence-positive cell × mean fluorescence intensity of background signal). Median CTCF of quantified areas in representative images are depicted as white numbers. IgA and IgM levels in ( c ) bronchoalveolar lavage fluid (BALF) and ( d ) serum of SPC-HA and SPC-HAxTCR-HA mice were determined by ELISA. ( e ) Relative secretory IgA concentrations in serial dilutions of BALF samples were determined by ELISA. Results are expressed as the mean optical density (OD) at 450 nm ± SEM, *p

    Journal: Scientific Reports

    Article Title: Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance

    doi: 10.1038/s41598-017-05212-4

    Figure Lengend Snippet: Increased mucosal transport of secretory antibodies in inflamed lungs. ( a ) anti-pIgR and anti-Gapdh immunoblot of 20 µg protein from whole lung homogenates of SPC-HA (n = 3) and SPC-HAxTCR-HA (n = 3) mice. Densitometric quantification of protein bands is stated in arbitrary units above each lane. Relative pIgR quantity was calculated normalizing densitometric pIgR value to the corresponding Gapdh value and subsequently comparing normalized pIgR values of the SPC-HAxTCR-HA group to the SPC-HA group. Data are representative for at least two individual experiments with similar results. ( b ) Lung tissue sections were stained with anti-pIgR (green), representative alveolar structures from n = 3/group are depicted. White circles illustrate representative densitometrically quantified tissue areas. Calculated total cell fluorescence (CTCF) was determined as: Integrated density of fluorescence-positive cell – (Area of fluorescence-positive cell × mean fluorescence intensity of background signal). Median CTCF of quantified areas in representative images are depicted as white numbers. IgA and IgM levels in ( c ) bronchoalveolar lavage fluid (BALF) and ( d ) serum of SPC-HA and SPC-HAxTCR-HA mice were determined by ELISA. ( e ) Relative secretory IgA concentrations in serial dilutions of BALF samples were determined by ELISA. Results are expressed as the mean optical density (OD) at 450 nm ± SEM, *p

    Article Snippet: ELISA ELISA kits for mouse IgA and IgM (eBioscience, Frankfurt am Main, Germany), mouse IL-6 and TNF-α (BioLegend, London, United Kingdom), mouse C5a (R & D Systems, Minneapolis, MN) and mouse albumin (Bethyl Laboratories Inc., Montogomery, TX) were used according to manufacturer’s instructions.

    Techniques: Mouse Assay, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay

    Comparative titres of mucosal IgA. Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific ELISA. Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P

    Journal: Frontiers in Microbiology

    Article Title: A Long-Term Efficacy Trial of a Live, Attenuated Salmonella Typhimurium Vaccine in Layer Hens

    doi: 10.3389/fmicb.2018.01380

    Figure Lengend Snippet: Comparative titres of mucosal IgA. Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific ELISA. Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P

    Article Snippet: Secretory IgA was measured using chicken IgA ELISA kit (Abcam ab157691).

    Techniques: Enzyme-linked Immunosorbent Assay

    Detection of IgA production using ELISA in tears (a) and tracheal wash (b) of chickens ( n = 5 per group) vaccinated with live H120 alone (group I) or in combination with CR88 (group II) at day 1. The two groups were again vaccinated with CR88 at 14 days

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Mucosal, Cellular, and Humoral Immune Responses Induced by Different Live Infectious Bronchitis Virus Vaccination Regimes and Protection Conferred against Infectious Bronchitis Virus Q1 Strain

    doi: 10.1128/CVI.00368-15

    Figure Lengend Snippet: Detection of IgA production using ELISA in tears (a) and tracheal wash (b) of chickens ( n = 5 per group) vaccinated with live H120 alone (group I) or in combination with CR88 (group II) at day 1. The two groups were again vaccinated with CR88 at 14 days

    Article Snippet: To detect IBV antibodies, serum samples were tested with a commercial IBV enzyme-linked immunosorbent assay (ELISA) kit (FlockChek; IDEXX Laboratories, Inc., Westbrook, ME, USA), and immunoglobulin A (IgA) in tears and tracheal washes was assayed using a commercial IgA chicken ELISA kit (Abcam, Cambridge, United Kingdom).

    Techniques: Enzyme-linked Immunosorbent Assay

    IgA-Binding of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and ELISA. (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p

    Journal: Cell

    Article Title: Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10

    doi: 10.1016/j.cell.2018.11.035

    Figure Lengend Snippet: IgA-Binding of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and ELISA. (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p

    Article Snippet: One million isolated fecal bacteria per individual were coated in duplicates and probed for IgA using a commercially available quantitative ELISA Assay (Human IgA ELISA Kit, Abcam).

    Techniques: Binding Assay, Mass Spectrometry, Isolation, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Staining

    Thymic stromal lymphopoietin (TSLP) enhanced IgA production in follicular dendritic cell (FDC)-associated clusters through TSLP receptor (TSLPR), and serum IgA and TSLP concentrations (A) Left to right in the upper row: morphology, CD10 + GC cells and CD21 + FDCs in FDC-associated clusters isolated from tonsillar GCs of IgAN patients. FDC-associated clusters are composed of CD 10 + GC cells and CD21 + FDCs, with about 1 FDC per 10 lymphocytes in each FDC-associated cluster. (B, C) Left to right in the lower row: IgA and TSLP concentrations in the supernatants of FDC-associated clusters. IgA and TSLP were quantified in the supernatants using ELISA. Combined data (mean ± SD) from experiments using FDC-associated clusters from 3 IgAN patients are presented. (D, E) The IgA and TSLP concentrations in the sera of 20 IgAN patients with Tripterygium Wilfordii treatment, 20 IgAN patients without treatment, 20 non-IgAN patients with chronic tonsillitis patients, and 20 healthy volunteers were measured by ELISA. Error bars show means ± SEMs. * , P

    Journal: Oncotarget

    Article Title: Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy

    doi: 10.18632/oncotarget.22561

    Figure Lengend Snippet: Thymic stromal lymphopoietin (TSLP) enhanced IgA production in follicular dendritic cell (FDC)-associated clusters through TSLP receptor (TSLPR), and serum IgA and TSLP concentrations (A) Left to right in the upper row: morphology, CD10 + GC cells and CD21 + FDCs in FDC-associated clusters isolated from tonsillar GCs of IgAN patients. FDC-associated clusters are composed of CD 10 + GC cells and CD21 + FDCs, with about 1 FDC per 10 lymphocytes in each FDC-associated cluster. (B, C) Left to right in the lower row: IgA and TSLP concentrations in the supernatants of FDC-associated clusters. IgA and TSLP were quantified in the supernatants using ELISA. Combined data (mean ± SD) from experiments using FDC-associated clusters from 3 IgAN patients are presented. (D, E) The IgA and TSLP concentrations in the sera of 20 IgAN patients with Tripterygium Wilfordii treatment, 20 IgAN patients without treatment, 20 non-IgAN patients with chronic tonsillitis patients, and 20 healthy volunteers were measured by ELISA. Error bars show means ± SEMs. * , P

    Article Snippet: After collection of supernatants, qualitative detection of TSLP and IgA in cell supernatants was performed using Human TSLP ELISA MAX Deluxe Sets (Biolegend, San Diego, CA, USA) and IgA Human ELISA Kit (ab137980; Abcam).

    Techniques: Isolation, Enzyme-linked Immunosorbent Assay

    Flow of patients through the trial. CD, coeliac disease; DGP, deamidated gliadin peptide; EMA IgA, endomysial antibody IgA; GIT, gastrointestinal disorders; HLA, human leukocyte antigen; NAFLD, non-alcoholic fatty liver disease; NASH, non-alcoholic steatohepatitis; TE, transient elastography; TTGA, tissue transglutaminase antibody.

    Journal: BMJ Open Gastroenterology

    Article Title: Clinicopathological and immunological characteristics and outcome of concomitant coeliac disease and non-alcoholic fatty liver disease in adults: a large prospective longitudinal study

    doi: 10.1136/bmjgast-2017-000150

    Figure Lengend Snippet: Flow of patients through the trial. CD, coeliac disease; DGP, deamidated gliadin peptide; EMA IgA, endomysial antibody IgA; GIT, gastrointestinal disorders; HLA, human leukocyte antigen; NAFLD, non-alcoholic fatty liver disease; NASH, non-alcoholic steatohepatitis; TE, transient elastography; TTGA, tissue transglutaminase antibody.

    Article Snippet: Patients with symptoms suggestive of CD but negative TTGA/EMA results were tested for potential IgA deficiency (Abcam human IgA ELISA Kit).

    Techniques: Flow Cytometry

    Bacteriophages act to induce both commensal and phage specific immunity. (A) IFN-γ measured by ELISA in the supernatant of BMDCs from MHCII −/− mice pulsed with phage for 24 hours and co-cultured with WT T cells for 72 hours. (B) IFN-γ measured by ELISA in the supernatant of BMDCs from WT mice pulsed with antigens and ovalbumin (100 μg/mL) and co-cultured with CD4+ T cells from OTII mice. (C, D) Experimental set-up of Phage-Ovalbumin co-culture experiments. IFN-γ was measured in the supernatant by ELISA. (E) Feces from bacteriophage cocktail treated germfree mice assayed for phage specific IgA by ELISA. Data are represented as mean ± SEM. *p

    Journal: Cell host & microbe

    Article Title: Expansion of bacteriophages is linked to aggravated intestinal inflammation and colitis

    doi: 10.1016/j.chom.2019.01.008

    Figure Lengend Snippet: Bacteriophages act to induce both commensal and phage specific immunity. (A) IFN-γ measured by ELISA in the supernatant of BMDCs from MHCII −/− mice pulsed with phage for 24 hours and co-cultured with WT T cells for 72 hours. (B) IFN-γ measured by ELISA in the supernatant of BMDCs from WT mice pulsed with antigens and ovalbumin (100 μg/mL) and co-cultured with CD4+ T cells from OTII mice. (C, D) Experimental set-up of Phage-Ovalbumin co-culture experiments. IFN-γ was measured in the supernatant by ELISA. (E) Feces from bacteriophage cocktail treated germfree mice assayed for phage specific IgA by ELISA. Data are represented as mean ± SEM. *p

    Article Snippet: Final supernatants were used as samples for an IgA specific ELISA kit (eBioscience).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Cell Culture, Co-Culture Assay

    PD-1 + and PD-1 Neg Tfr cells suppress antibody responses in vitro. (A) Flow cytometry plots showing the percentage of cells producing IL-10 or IL-21 within the indicated populations. All cells were first gated on live CD4 + CD45RA − T cells (not depicted), then CXCR5 and PD-1, and followed by FOXP3 and CD25. LMNCs were activated with PMA/ionomycin. Unstimulated controls were used to draw cytokine gates. Percentage of IL-10 (B) or IL-21 (C) producing cells within T cell subsets as determined by flow cytometry and gated in A. Data are pooled from three similar experiments, n = 14 subjects. (D) CD40L flow cytometry histograms. (E) Percentage of T cells that are CD40L + based on gates drawn on unstimulated controls. Data are pooled from two similar experiments, n = 10 subjects. Analysis of IgG (F) or IgA (G) secretion by ELISA after stimulation with SEB in coculture with a 3:1 ratio of Tfh cells and B cells or 3:1:1 ratio of sorted Tfh cells, PD-1 + Tfr cells, and B cells for 7 d; n = 19 subjects (F) or n = 6 subjects (G). (H) IgG secretion from Tfh cell–free coculture experiments using mitogen-activated B cells with or without PD-1 + Tfr cells at a 1:1 ratio; n = 5. (I) IgG secretion using sorted Tfh, PD-1 + Tfr cells, PD-1 Neg Tfr cells, or Treg cells at indicated ratios for 7 d n = 6–10 subjects. Not depicted: Differences between the “Tfh + B” and all other groups were highly significant. For F–I, antibody concentrations are expressed as a percentage of the corresponding “Tfh + B” group titers. Paired t tests; mean ± SEM; *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Spatial distribution and function of T follicular regulatory cells in human lymph nodes

    doi: 10.1084/jem.20171940

    Figure Lengend Snippet: PD-1 + and PD-1 Neg Tfr cells suppress antibody responses in vitro. (A) Flow cytometry plots showing the percentage of cells producing IL-10 or IL-21 within the indicated populations. All cells were first gated on live CD4 + CD45RA − T cells (not depicted), then CXCR5 and PD-1, and followed by FOXP3 and CD25. LMNCs were activated with PMA/ionomycin. Unstimulated controls were used to draw cytokine gates. Percentage of IL-10 (B) or IL-21 (C) producing cells within T cell subsets as determined by flow cytometry and gated in A. Data are pooled from three similar experiments, n = 14 subjects. (D) CD40L flow cytometry histograms. (E) Percentage of T cells that are CD40L + based on gates drawn on unstimulated controls. Data are pooled from two similar experiments, n = 10 subjects. Analysis of IgG (F) or IgA (G) secretion by ELISA after stimulation with SEB in coculture with a 3:1 ratio of Tfh cells and B cells or 3:1:1 ratio of sorted Tfh cells, PD-1 + Tfr cells, and B cells for 7 d; n = 19 subjects (F) or n = 6 subjects (G). (H) IgG secretion from Tfh cell–free coculture experiments using mitogen-activated B cells with or without PD-1 + Tfr cells at a 1:1 ratio; n = 5. (I) IgG secretion using sorted Tfh, PD-1 + Tfr cells, PD-1 Neg Tfr cells, or Treg cells at indicated ratios for 7 d n = 6–10 subjects. Not depicted: Differences between the “Tfh + B” and all other groups were highly significant. For F–I, antibody concentrations are expressed as a percentage of the corresponding “Tfh + B” group titers. Paired t tests; mean ± SEM; *, P

    Article Snippet: IgA was quantified using IgA human ELISA kits (Thermo Fisher Scientific).

    Techniques: In Vitro, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    IgA increases at multiple mucosal sites after recolonization of GF SW mice with Bacteroides acidifaciens. GF SW mice (n = 5–7) were orally gavaged with (1 × 10 8 CFU/mL) B. acidifaciens and kept in GF housing for 21 days. (A) Significance of changes in the colon IgA gene transcripts over time was determined by 1-way ANOVA followed by Dunn's comparison test. (B) Stool samples were assayed for SIgA by ELISA. Significance of changes in gut SIgA over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (C) Changes in IgA transcript levels in the small-intestine samples. Significance was determined by Student's t-test. (D) Significance of changes in LG IgA transcripts over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (E) Pooled eyewash samples were collected from mice and analyzed for IgA by ELISA. Significance of changes in eyewash SIgA over time was determined by 1-way ANOVA followed by Dunn's comparison test. Cumulatively, the data show that gut reconstitution of GF SW mice with B. acidifaciens induced a robust gut and ocular IgA transcription.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Role of Microbiota in Strengthening Ocular Mucosal Barrier Function Through Secretory IgA

    doi: 10.1167/iovs.17-22119

    Figure Lengend Snippet: IgA increases at multiple mucosal sites after recolonization of GF SW mice with Bacteroides acidifaciens. GF SW mice (n = 5–7) were orally gavaged with (1 × 10 8 CFU/mL) B. acidifaciens and kept in GF housing for 21 days. (A) Significance of changes in the colon IgA gene transcripts over time was determined by 1-way ANOVA followed by Dunn's comparison test. (B) Stool samples were assayed for SIgA by ELISA. Significance of changes in gut SIgA over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (C) Changes in IgA transcript levels in the small-intestine samples. Significance was determined by Student's t-test. (D) Significance of changes in LG IgA transcripts over time was determined by 1-way ANOVA followed by Dunnett's comparison test. (E) Pooled eyewash samples were collected from mice and analyzed for IgA by ELISA. Significance of changes in eyewash SIgA over time was determined by 1-way ANOVA followed by Dunn's comparison test. Cumulatively, the data show that gut reconstitution of GF SW mice with B. acidifaciens induced a robust gut and ocular IgA transcription.

    Article Snippet: IgA ELISA SIgA was quantified by using Mouse IgA ELISA Ready-SET-Go (eBioscience, Vienna, Austria) per manufacturer's instructions.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Systemic IL-1β blockade modulates IgA production in the LG. (A) ELISA for serum IL-1β levels in GF SW and SPF SW mice. Significance was determined by a Student's t-test. Cervical lymph nodes (B) and LGs (C) were harvested from SPF SW mice after systemic IL-1β antibody treatment and analyzed for IgA gene expression by using qRT-PCR. Significance was determined by a Student's t-test. Control mice received isotype control treatment. (D) Pooled eyewash samples were collected 24 hours after administration of an IL-1β blockade and were assayed for SIgA by ELISA. Significance was determined by a Student's t-test. Cumulatively, data show that ocular SIgA concentration at steady state depends on IL-1β signaling.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Role of Microbiota in Strengthening Ocular Mucosal Barrier Function Through Secretory IgA

    doi: 10.1167/iovs.17-22119

    Figure Lengend Snippet: Systemic IL-1β blockade modulates IgA production in the LG. (A) ELISA for serum IL-1β levels in GF SW and SPF SW mice. Significance was determined by a Student's t-test. Cervical lymph nodes (B) and LGs (C) were harvested from SPF SW mice after systemic IL-1β antibody treatment and analyzed for IgA gene expression by using qRT-PCR. Significance was determined by a Student's t-test. Control mice received isotype control treatment. (D) Pooled eyewash samples were collected 24 hours after administration of an IL-1β blockade and were assayed for SIgA by ELISA. Significance was determined by a Student's t-test. Cumulatively, data show that ocular SIgA concentration at steady state depends on IL-1β signaling.

    Article Snippet: IgA ELISA SIgA was quantified by using Mouse IgA ELISA Ready-SET-Go (eBioscience, Vienna, Austria) per manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Expressing, Quantitative RT-PCR, Concentration Assay

    The flow of participants through each stage of this randomised trial. P, Prednisolone tTg: tissue transglutaminase; ELISA: enzyme-linked immunosorbent assay; n: number; ESPGHAN: European Society for Paediatric Gastroenterology, Hepatology and Nutrition; GFD: gluten-free diet; GFD+P: gluten-free diet, plus prednisolone

    Journal: Cureus

    Article Title: Addition of a Short Course of Prednisolone to a Gluten-Free Diet vs. Gluten-Free Diet Alone in Recovery of Celiac Disease: A Pilot Randomized Controlled Trial

    doi: 10.7759/cureus.2118

    Figure Lengend Snippet: The flow of participants through each stage of this randomised trial. P, Prednisolone tTg: tissue transglutaminase; ELISA: enzyme-linked immunosorbent assay; n: number; ESPGHAN: European Society for Paediatric Gastroenterology, Hepatology and Nutrition; GFD: gluten-free diet; GFD+P: gluten-free diet, plus prednisolone

    Article Snippet: IgA anti-tTg antibody measurements were done by using a Thermo® iEMS™ microplate reader with an anti-tissue transglutaminase IgA ELISA kit (Thermo Scientific, Waltham, Massachusetts, USA).

    Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

    CXCR5 + CD8 + T Cells provide help to B cells for the production of immunoglobulins. Physical contact between CD8 + T Cells (red) and B cells (green) in tonsil sections, and scale bars, 50μm. (A , n = 5). Sorted tonsil B cells and sorted CD8 + T Cells at the ratio of 1:1, 1:5, and 1:10 were co-cultured with or without α-CD3/CD28 dynabeads for 10 days (B,C) . Sorted B cells were co-cultured with fresh CD8 + or fixed CD8 + T Cells, fresh CD4 + or fixed CD4 + T Cells at the ratio of 5:1 in the presence of α-CD3/CD28 dynabeads for 10 days (D) . Sorted B cells and CD8 + , CXCR5 + CD8 + , CXCR5 − CD8 + T Cells at the ratio of 5:1 were co-cultured with or without anti-IL-21 and anti-CD40L in the presence of α-CD3/CD28 dynabeads for 10 days (E) . The supernatants from the different co-cultures were analyzed by ELISA for the production of IgG, IgM, and IgA. Data are expressed as the mean ± SD, and compared with Mann–Whitney test. * P

    Journal: Frontiers in Immunology

    Article Title: A Subset of CXCR5+CD8+ T Cells in the Germinal Centers From Human Tonsils and Lymph Nodes Help B Cells Produce Immunoglobulins

    doi: 10.3389/fimmu.2018.02287

    Figure Lengend Snippet: CXCR5 + CD8 + T Cells provide help to B cells for the production of immunoglobulins. Physical contact between CD8 + T Cells (red) and B cells (green) in tonsil sections, and scale bars, 50μm. (A , n = 5). Sorted tonsil B cells and sorted CD8 + T Cells at the ratio of 1:1, 1:5, and 1:10 were co-cultured with or without α-CD3/CD28 dynabeads for 10 days (B,C) . Sorted B cells were co-cultured with fresh CD8 + or fixed CD8 + T Cells, fresh CD4 + or fixed CD4 + T Cells at the ratio of 5:1 in the presence of α-CD3/CD28 dynabeads for 10 days (D) . Sorted B cells and CD8 + , CXCR5 + CD8 + , CXCR5 − CD8 + T Cells at the ratio of 5:1 were co-cultured with or without anti-IL-21 and anti-CD40L in the presence of α-CD3/CD28 dynabeads for 10 days (E) . The supernatants from the different co-cultures were analyzed by ELISA for the production of IgG, IgM, and IgA. Data are expressed as the mean ± SD, and compared with Mann–Whitney test. * P

    Article Snippet: For the ELISA assay of immunoglobulins, co-culture supernatants from B cells and T cells were harvested, and the levels of IgG, IgM, and IgA were measured by Human IgG, IgM or IgA ELISA Ready-SET-Go!® (eBioscience).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    The representative figure showed that SO 2 inhalation did not affect IgG levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : IgA level in the serum; f : IgA level in the nasal septum

    Journal: Journal of Occupational Medicine and Toxicology (London, England)

    Article Title: Sulfur dioxide exposure reduces the quantity of CD19+ cells and causes nasal epithelial injury in rats

    doi: 10.1186/s12995-018-0205-x

    Figure Lengend Snippet: The representative figure showed that SO 2 inhalation did not affect IgG levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : IgA level in the serum; f : IgA level in the nasal septum

    Article Snippet: Rat IgG, IgA and IgE ELISA kit was purchased from eBioscience Corp. (USA).

    Techniques: