iga elisas Search Results


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  • 93
    Thermo Fisher mouse iga
    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse <t>IgA</t> or <t>anti-IgM</t> antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p
    Mouse Iga, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad iga enzyme linked immunosorbent assay
    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse <t>IgA</t> or <t>anti-IgM</t> antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p
    Iga Enzyme Linked Immunosorbent Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bethyl iga elisas
    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse <t>IgA</t> or <t>anti-IgM</t> antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p
    Iga Elisas, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher iga elisas
    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse <t>IgA</t> or <t>anti-IgM</t> antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p
    Iga Elisas, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bethyl iga enzyme linked immunosorbent assay elisa
    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse <t>IgA</t> or <t>anti-IgM</t> antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p
    Iga Enzyme Linked Immunosorbent Assay Elisa, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Abcam iga mouse enzyme linked immunosorbent assay elisa kits
    AHR-dependent inflammation in mice after dietary exposure to vehicle or TCDF (24 μg/kg). ( A ) qPCR analysis of inflammatory cytokine ( IL-1 β, TNF -α, IL-10, Saa1, and Saa3 ) mRNA expression in the ileum of Ahr +/+ mice. ( B ) IL-1 β and Tnf- α expression in the ileum of Ahr –/– mice. ( C ) qPCR analysis of Lcn-2 mRNA expression in the ileum of Ahr +/+ and Ahr –/– mice. ( D ) Quantification of fecal LCN2 in Ahr +/+ mice by <t>ELISA.</t> ( E ) qPCR analysis of Myosin Vb and Ptprh mRNA in the ileum of Ahr +/+ mice after TCDF treatment. ( F ) Quantification of serum LPS in Ahr +/+ mice. ( G ) Quantification of <t>IgA</t> in Ahr +/+ mice by ELISA. Data are presented as mean ± SD; n = 5/group. NS, not significant. * p
    Iga Mouse Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam iga enzyme linked immunosorbent assay elisa kit
    Comparative titres of mucosal <t>IgA.</t> Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific <t>ELISA.</t> Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P
    Iga Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Abcam iga human enzyme linked immunosorbent assay
    Comparative titres of mucosal <t>IgA.</t> Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific <t>ELISA.</t> Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P
    Iga Human Enzyme Linked Immunosorbent Assay, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bethyl iga elisa quantitation kit
    Effects of scFOS in the maternal diet on colostral and milk <t>IgA,</t> IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by <t>ELISA</t> assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05
    Iga Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bethyl mouse iga enzyme linked immunosorbent assay elisa quantitation set
    Fecal concentration of secretory <t>IgA</t> before and after LAB administration. The fecal concentration of secretory IgA before and after administration of C59 or GG during experiment 2 was determined using <t>ELISA.</t> The white and gray bars represent the concentration of secretory IgA (μg/ml) before and after LAB administration, respectively. The data are shown as the least-squares means ± SE (n = 5 in each group).
    Mouse Iga Enzyme Linked Immunosorbent Assay Elisa Quantitation Set, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Inova Diagnostics quanta lite r h ttg iga enzyme linked immunosorbent assay elisa
    Fecal concentration of secretory <t>IgA</t> before and after LAB administration. The fecal concentration of secretory IgA before and after administration of C59 or GG during experiment 2 was determined using <t>ELISA.</t> The white and gray bars represent the concentration of secretory IgA (μg/ml) before and after LAB administration, respectively. The data are shown as the least-squares means ± SE (n = 5 in each group).
    Quanta Lite R H Ttg Iga Enzyme Linked Immunosorbent Assay Elisa, supplied by Inova Diagnostics, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 5 article reviews
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    84
    Elabscience human iga immunoglobulin a elisa kit
    Boxplot <t>IgA</t> level before and after treatment.
    Human Iga Immunoglobulin A Elisa Kit, supplied by Elabscience, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MIKROGEN iga elisas
    Boxplot <t>IgA</t> level before and after treatment.
    Iga Elisas, supplied by MIKROGEN, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Inova Diagnostics iga httg enzyme linked immunosorbent assay kit
    Boxplot <t>IgA</t> level before and after treatment.
    Iga Httg Enzyme Linked Immunosorbent Assay Kit, supplied by Inova Diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Greiner Bio iga2 elisa elisa plates
    BAFF and Retinoic acid differentially affect IgA1 and <t>IgA2</t> production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific <t>ELISA.</t> Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p
    Iga2 Elisa Elisa Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Bethyl horse iga enzyme linked immunosorbent assay elisa quantification set
    BAFF and Retinoic acid differentially affect IgA1 and <t>IgA2</t> production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific <t>ELISA.</t> Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p
    Horse Iga Enzyme Linked Immunosorbent Assay Elisa Quantification Set, supplied by Bethyl, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Virion\Serion serion enzyme linked immunosorbent assay elisa classic candida albicans iga igg igm
    BAFF and Retinoic acid differentially affect IgA1 and <t>IgA2</t> production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific <t>ELISA.</t> Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p
    Serion Enzyme Linked Immunosorbent Assay Elisa Classic Candida Albicans Iga Igg Igm, supplied by Virion\Serion, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam pig iga elisa kit
    BAFF and Retinoic acid differentially affect IgA1 and <t>IgA2</t> production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific <t>ELISA.</t> Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p
    Pig Iga Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Immunology Consultant Laboratory iga elisa kit
    Mesalamine increases fecal <t>IgA</t> production. ( A ) WT (n = 6 mice per group) and AhR -/- mice (n = 4 mice per group), or ( B ) bone marrow chimeric mice (BM-AhR +/+ mice and BM-AhR -/- mice) (n = 10 mice per group) were orally treated with 50 mg/kg of mesalamine for 2 weeks. Concentrations of IgA of stool samples were measured by <t>ELISA.</t> Error bars represent mean ± SEM. * P
    Iga Elisa Kit, supplied by Immunology Consultant Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam iga elisa kits
    Thymic stromal lymphopoietin (TSLP) enhanced <t>IgA</t> production in follicular dendritic cell (FDC)-associated clusters through TSLP receptor (TSLPR), and serum IgA and TSLP concentrations (A) Left to right in the upper row: morphology, CD10 + GC cells and CD21 + FDCs in FDC-associated clusters isolated from tonsillar GCs of IgAN patients. FDC-associated clusters are composed of CD 10 + GC cells and CD21 + FDCs, with about 1 FDC per 10 lymphocytes in each FDC-associated cluster. (B, C) Left to right in the lower row: IgA and TSLP concentrations in the supernatants of FDC-associated clusters. IgA and TSLP were quantified in the supernatants using <t>ELISA.</t> Combined data (mean ± SD) from experiments using FDC-associated clusters from 3 IgAN patients are presented. (D, E) The IgA and TSLP concentrations in the sera of 20 IgAN patients with Tripterygium Wilfordii treatment, 20 IgAN patients without treatment, 20 non-IgAN patients with chronic tonsillitis patients, and 20 healthy volunteers were measured by ELISA. Error bars show means ± SEMs. * , P
    Iga Elisa Kits, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse IgA or anti-IgM antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p

    Journal: Scientific Reports

    Article Title: Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance

    doi: 10.1038/s41598-017-05212-4

    Figure Lengend Snippet: Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse IgA or anti-IgM antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p

    Article Snippet: ELISA ELISA kits for mouse IgA and IgM (eBioscience, Frankfurt am Main, Germany), mouse IL-6 and TNF-α (BioLegend, London, United Kingdom), mouse C5a (R & D Systems, Minneapolis, MN) and mouse albumin (Bethyl Laboratories Inc., Montogomery, TX) were used according to manufacturer’s instructions.

    Techniques: Binding Assay, Incubation, Mouse Assay, Staining, Flow Cytometry, Cytometry, FACS, Fluorescence

    Increased mucosal transport of secretory antibodies in inflamed lungs. ( a ) anti-pIgR and anti-Gapdh immunoblot of 20 µg protein from whole lung homogenates of SPC-HA (n = 3) and SPC-HAxTCR-HA (n = 3) mice. Densitometric quantification of protein bands is stated in arbitrary units above each lane. Relative pIgR quantity was calculated normalizing densitometric pIgR value to the corresponding Gapdh value and subsequently comparing normalized pIgR values of the SPC-HAxTCR-HA group to the SPC-HA group. Data are representative for at least two individual experiments with similar results. ( b ) Lung tissue sections were stained with anti-pIgR (green), representative alveolar structures from n = 3/group are depicted. White circles illustrate representative densitometrically quantified tissue areas. Calculated total cell fluorescence (CTCF) was determined as: Integrated density of fluorescence-positive cell – (Area of fluorescence-positive cell × mean fluorescence intensity of background signal). Median CTCF of quantified areas in representative images are depicted as white numbers. IgA and IgM levels in ( c ) bronchoalveolar lavage fluid (BALF) and ( d ) serum of SPC-HA and SPC-HAxTCR-HA mice were determined by ELISA. ( e ) Relative secretory IgA concentrations in serial dilutions of BALF samples were determined by ELISA. Results are expressed as the mean optical density (OD) at 450 nm ± SEM, *p

    Journal: Scientific Reports

    Article Title: Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance

    doi: 10.1038/s41598-017-05212-4

    Figure Lengend Snippet: Increased mucosal transport of secretory antibodies in inflamed lungs. ( a ) anti-pIgR and anti-Gapdh immunoblot of 20 µg protein from whole lung homogenates of SPC-HA (n = 3) and SPC-HAxTCR-HA (n = 3) mice. Densitometric quantification of protein bands is stated in arbitrary units above each lane. Relative pIgR quantity was calculated normalizing densitometric pIgR value to the corresponding Gapdh value and subsequently comparing normalized pIgR values of the SPC-HAxTCR-HA group to the SPC-HA group. Data are representative for at least two individual experiments with similar results. ( b ) Lung tissue sections were stained with anti-pIgR (green), representative alveolar structures from n = 3/group are depicted. White circles illustrate representative densitometrically quantified tissue areas. Calculated total cell fluorescence (CTCF) was determined as: Integrated density of fluorescence-positive cell – (Area of fluorescence-positive cell × mean fluorescence intensity of background signal). Median CTCF of quantified areas in representative images are depicted as white numbers. IgA and IgM levels in ( c ) bronchoalveolar lavage fluid (BALF) and ( d ) serum of SPC-HA and SPC-HAxTCR-HA mice were determined by ELISA. ( e ) Relative secretory IgA concentrations in serial dilutions of BALF samples were determined by ELISA. Results are expressed as the mean optical density (OD) at 450 nm ± SEM, *p

    Article Snippet: ELISA ELISA kits for mouse IgA and IgM (eBioscience, Frankfurt am Main, Germany), mouse IL-6 and TNF-α (BioLegend, London, United Kingdom), mouse C5a (R & D Systems, Minneapolis, MN) and mouse albumin (Bethyl Laboratories Inc., Montogomery, TX) were used according to manufacturer’s instructions.

    Techniques: Mouse Assay, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay

    Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial IgA and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by ELISA. Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p

    Journal: Journal of diabetes and its complications

    Article Title: Endothelial Cell-Specific Overexpression of Endothelial Nitric Oxide Synthase in Ins2Akita Mice Exacerbates Diabetic Nephropathy

    doi: 10.1016/j.jdiacomp.2018.10.003

    Figure Lengend Snippet: Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial IgA and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by ELISA. Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p

    Article Snippet: IgA levels were determined using a mouse IgA ELISA kit purchased from Thermoscientific (Frederick, MD).

    Techniques: Immunofluorescence, Mouse Assay, Staining, Electron Microscopy, Enzyme-linked Immunosorbent Assay

    AHR-dependent inflammation in mice after dietary exposure to vehicle or TCDF (24 μg/kg). ( A ) qPCR analysis of inflammatory cytokine ( IL-1 β, TNF -α, IL-10, Saa1, and Saa3 ) mRNA expression in the ileum of Ahr +/+ mice. ( B ) IL-1 β and Tnf- α expression in the ileum of Ahr –/– mice. ( C ) qPCR analysis of Lcn-2 mRNA expression in the ileum of Ahr +/+ and Ahr –/– mice. ( D ) Quantification of fecal LCN2 in Ahr +/+ mice by ELISA. ( E ) qPCR analysis of Myosin Vb and Ptprh mRNA in the ileum of Ahr +/+ mice after TCDF treatment. ( F ) Quantification of serum LPS in Ahr +/+ mice. ( G ) Quantification of IgA in Ahr +/+ mice by ELISA. Data are presented as mean ± SD; n = 5/group. NS, not significant. * p

    Journal: Environmental Health Perspectives

    Article Title: Persistent Organic Pollutants Modify Gut Microbiota–Host Metabolic Homeostasis in Mice Through Aryl Hydrocarbon Receptor Activation

    doi: 10.1289/ehp.1409055

    Figure Lengend Snippet: AHR-dependent inflammation in mice after dietary exposure to vehicle or TCDF (24 μg/kg). ( A ) qPCR analysis of inflammatory cytokine ( IL-1 β, TNF -α, IL-10, Saa1, and Saa3 ) mRNA expression in the ileum of Ahr +/+ mice. ( B ) IL-1 β and Tnf- α expression in the ileum of Ahr –/– mice. ( C ) qPCR analysis of Lcn-2 mRNA expression in the ileum of Ahr +/+ and Ahr –/– mice. ( D ) Quantification of fecal LCN2 in Ahr +/+ mice by ELISA. ( E ) qPCR analysis of Myosin Vb and Ptprh mRNA in the ileum of Ahr +/+ mice after TCDF treatment. ( F ) Quantification of serum LPS in Ahr +/+ mice. ( G ) Quantification of IgA in Ahr +/+ mice by ELISA. Data are presented as mean ± SD; n = 5/group. NS, not significant. * p

    Article Snippet: We obtained IgA mouse enzyme-linked immunosorbent assay (ELISA) kits from Abcam, and Pierce LAL chromogenic endotoxin quantitation kits and lipocalin-2 (LCN2) ELISA kits from Thermo Scientific.

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay

    Comparative titres of mucosal IgA. Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific ELISA. Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P

    Journal: Frontiers in Microbiology

    Article Title: A Long-Term Efficacy Trial of a Live, Attenuated Salmonella Typhimurium Vaccine in Layer Hens

    doi: 10.3389/fmicb.2018.01380

    Figure Lengend Snippet: Comparative titres of mucosal IgA. Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific ELISA. Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P

    Article Snippet: Secretory IgA was measured using chicken IgA ELISA kit (Abcam ab157691).

    Techniques: Enzyme-linked Immunosorbent Assay

    Detection of IgA production using ELISA in tears (a) and tracheal wash (b) of chickens ( n = 5 per group) vaccinated with live H120 alone (group I) or in combination with CR88 (group II) at day 1. The two groups were again vaccinated with CR88 at 14 days

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Mucosal, Cellular, and Humoral Immune Responses Induced by Different Live Infectious Bronchitis Virus Vaccination Regimes and Protection Conferred against Infectious Bronchitis Virus Q1 Strain

    doi: 10.1128/CVI.00368-15

    Figure Lengend Snippet: Detection of IgA production using ELISA in tears (a) and tracheal wash (b) of chickens ( n = 5 per group) vaccinated with live H120 alone (group I) or in combination with CR88 (group II) at day 1. The two groups were again vaccinated with CR88 at 14 days

    Article Snippet: To detect IBV antibodies, serum samples were tested with a commercial IBV enzyme-linked immunosorbent assay (ELISA) kit (FlockChek; IDEXX Laboratories, Inc., Westbrook, ME, USA), and immunoglobulin A (IgA) in tears and tracheal washes was assayed using a commercial IgA chicken ELISA kit (Abcam, Cambridge, United Kingdom).

    Techniques: Enzyme-linked Immunosorbent Assay

    Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05

    Journal: PLoS ONE

    Article Title: Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets

    doi: 10.1371/journal.pone.0107508

    Figure Lengend Snippet: Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05

    Article Snippet: Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Fecal concentration of secretory IgA before and after LAB administration. The fecal concentration of secretory IgA before and after administration of C59 or GG during experiment 2 was determined using ELISA. The white and gray bars represent the concentration of secretory IgA (μg/ml) before and after LAB administration, respectively. The data are shown as the least-squares means ± SE (n = 5 in each group).

    Journal: PLoS ONE

    Article Title: The distinct effects of orally administered Lactobacillus rhamnosus GG and Lactococcus lactis subsp. lactis C59 on gene expression in the murine small intestine

    doi: 10.1371/journal.pone.0188985

    Figure Lengend Snippet: Fecal concentration of secretory IgA before and after LAB administration. The fecal concentration of secretory IgA before and after administration of C59 or GG during experiment 2 was determined using ELISA. The white and gray bars represent the concentration of secretory IgA (μg/ml) before and after LAB administration, respectively. The data are shown as the least-squares means ± SE (n = 5 in each group).

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) Total IgA concentration in the fecal extracts or intestinal fluids was determined using a mouse IgA enzyme-linked immunosorbent assay (ELISA) quantitation set (Bethyl Laboratories, Inc., Montgomery, AL).

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    Boxplot IgA level before and after treatment.

    Journal: Advances in Preventive Medicine

    Article Title: Nephrolepis exaltata Herbal Mask Increases Nasal IgA Levels and Pulmonary Function in Textile Factory Workers

    doi: 10.1155/2019/5687135

    Figure Lengend Snippet: Boxplot IgA level before and after treatment.

    Article Snippet: After the above procedure, samples were assessed using ELISA (Enzyme-Linked Immunosorbent Assay) methodology with Human IgA (Immunoglobulin A) ELISA Kit from Elabscience® (catalog number E-EL-H1355).

    Techniques:

    Mean of delta score of IgA level between two groups.

    Journal: Advances in Preventive Medicine

    Article Title: Nephrolepis exaltata Herbal Mask Increases Nasal IgA Levels and Pulmonary Function in Textile Factory Workers

    doi: 10.1155/2019/5687135

    Figure Lengend Snippet: Mean of delta score of IgA level between two groups.

    Article Snippet: After the above procedure, samples were assessed using ELISA (Enzyme-Linked Immunosorbent Assay) methodology with Human IgA (Immunoglobulin A) ELISA Kit from Elabscience® (catalog number E-EL-H1355).

    Techniques:

    BAFF and Retinoic acid differentially affect IgA1 and IgA2 production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific ELISA. Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p

    Journal: European Journal of Immunology

    Article Title: BAFF augments IgA2 and IL‐10 production by TLR7/8 stimulated total peripheral blood B cells

    doi: 10.1002/eji.201646861

    Figure Lengend Snippet: BAFF and Retinoic acid differentially affect IgA1 and IgA2 production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific ELISA. Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p

    Article Snippet: IgA1 and IgA2 ELISA ELISA plates (# 650‐061, Greiner Bio One) were coated overnight with 1.0 μg/mL Polyclonal goat anti human IgA (Southern Biotech, 2050‐01).

    Techniques: Enzyme-linked Immunosorbent Assay

    Mesalamine increases fecal IgA production. ( A ) WT (n = 6 mice per group) and AhR -/- mice (n = 4 mice per group), or ( B ) bone marrow chimeric mice (BM-AhR +/+ mice and BM-AhR -/- mice) (n = 10 mice per group) were orally treated with 50 mg/kg of mesalamine for 2 weeks. Concentrations of IgA of stool samples were measured by ELISA. Error bars represent mean ± SEM. * P

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Induction of Colonic Regulatory T Cells by Mesalamine by Activating the Aryl Hydrocarbon Receptor

    doi: 10.1016/j.jcmgh.2017.03.010

    Figure Lengend Snippet: Mesalamine increases fecal IgA production. ( A ) WT (n = 6 mice per group) and AhR -/- mice (n = 4 mice per group), or ( B ) bone marrow chimeric mice (BM-AhR +/+ mice and BM-AhR -/- mice) (n = 10 mice per group) were orally treated with 50 mg/kg of mesalamine for 2 weeks. Concentrations of IgA of stool samples were measured by ELISA. Error bars represent mean ± SEM. * P

    Article Snippet: After centrifugation (4,000 × g , 20 minutes), supernatants were collected and IgA concentrations were measured using the IgA ELISA kit (ICL Lab, Portland, OR).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Thymic stromal lymphopoietin (TSLP) enhanced IgA production in follicular dendritic cell (FDC)-associated clusters through TSLP receptor (TSLPR), and serum IgA and TSLP concentrations (A) Left to right in the upper row: morphology, CD10 + GC cells and CD21 + FDCs in FDC-associated clusters isolated from tonsillar GCs of IgAN patients. FDC-associated clusters are composed of CD 10 + GC cells and CD21 + FDCs, with about 1 FDC per 10 lymphocytes in each FDC-associated cluster. (B, C) Left to right in the lower row: IgA and TSLP concentrations in the supernatants of FDC-associated clusters. IgA and TSLP were quantified in the supernatants using ELISA. Combined data (mean ± SD) from experiments using FDC-associated clusters from 3 IgAN patients are presented. (D, E) The IgA and TSLP concentrations in the sera of 20 IgAN patients with Tripterygium Wilfordii treatment, 20 IgAN patients without treatment, 20 non-IgAN patients with chronic tonsillitis patients, and 20 healthy volunteers were measured by ELISA. Error bars show means ± SEMs. * , P

    Journal: Oncotarget

    Article Title: Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy

    doi: 10.18632/oncotarget.22561

    Figure Lengend Snippet: Thymic stromal lymphopoietin (TSLP) enhanced IgA production in follicular dendritic cell (FDC)-associated clusters through TSLP receptor (TSLPR), and serum IgA and TSLP concentrations (A) Left to right in the upper row: morphology, CD10 + GC cells and CD21 + FDCs in FDC-associated clusters isolated from tonsillar GCs of IgAN patients. FDC-associated clusters are composed of CD 10 + GC cells and CD21 + FDCs, with about 1 FDC per 10 lymphocytes in each FDC-associated cluster. (B, C) Left to right in the lower row: IgA and TSLP concentrations in the supernatants of FDC-associated clusters. IgA and TSLP were quantified in the supernatants using ELISA. Combined data (mean ± SD) from experiments using FDC-associated clusters from 3 IgAN patients are presented. (D, E) The IgA and TSLP concentrations in the sera of 20 IgAN patients with Tripterygium Wilfordii treatment, 20 IgAN patients without treatment, 20 non-IgAN patients with chronic tonsillitis patients, and 20 healthy volunteers were measured by ELISA. Error bars show means ± SEMs. * , P

    Article Snippet: After collection of supernatants, qualitative detection of TSLP and IgA in cell supernatants was performed using Human TSLP ELISA MAX Deluxe Sets (Biolegend, San Diego, CA, USA) and IgA Human ELISA Kit (ab137980; Abcam).

    Techniques: Isolation, Enzyme-linked Immunosorbent Assay