iga elisa kit Search Results


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  • 93
    Thermo Fisher iga specific elisa kit
    Bacteriophages act to induce both commensal and phage specific immunity. (A) IFN-γ measured by <t>ELISA</t> in the supernatant of BMDCs from MHCII −/− mice pulsed with phage for 24 hours and co-cultured with WT T cells for 72 hours. (B) IFN-γ measured by ELISA in the supernatant of BMDCs from WT mice pulsed with antigens and ovalbumin (100 μg/mL) and co-cultured with CD4+ T cells from OTII mice. (C, D) Experimental set-up of Phage-Ovalbumin co-culture experiments. IFN-γ was measured in the supernatant by ELISA. (E) Feces from bacteriophage cocktail treated germfree mice assayed for phage specific <t>IgA</t> by ELISA. Data are represented as mean ± SEM. *p
    Iga Specific Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mouse iga elisa kit
    Bacteriophages act to induce both commensal and phage specific immunity. (A) IFN-γ measured by <t>ELISA</t> in the supernatant of BMDCs from MHCII −/− mice pulsed with phage for 24 hours and co-cultured with WT T cells for 72 hours. (B) IFN-γ measured by ELISA in the supernatant of BMDCs from WT mice pulsed with antigens and ovalbumin (100 μg/mL) and co-cultured with CD4+ T cells from OTII mice. (C, D) Experimental set-up of Phage-Ovalbumin co-culture experiments. IFN-γ was measured in the supernatant by ELISA. (E) Feces from bacteriophage cocktail treated germfree mice assayed for phage specific <t>IgA</t> by ELISA. Data are represented as mean ± SEM. *p
    Mouse Iga Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bethyl iga elisa quantitation kit
    Effects of scFOS in the maternal diet on colostral and milk <t>IgA,</t> IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by <t>ELISA</t> assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05
    Iga Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam iga mouse enzyme linked immunosorbent assay elisa kits
    AHR-dependent inflammation in mice after dietary exposure to vehicle or TCDF (24 μg/kg). ( A ) qPCR analysis of inflammatory cytokine ( IL-1 β, TNF -α, IL-10, Saa1, and Saa3 ) mRNA expression in the ileum of Ahr +/+ mice. ( B ) IL-1 β and Tnf- α expression in the ileum of Ahr –/– mice. ( C ) qPCR analysis of Lcn-2 mRNA expression in the ileum of Ahr +/+ and Ahr –/– mice. ( D ) Quantification of fecal LCN2 in Ahr +/+ mice by <t>ELISA.</t> ( E ) qPCR analysis of Myosin Vb and Ptprh mRNA in the ileum of Ahr +/+ mice after TCDF treatment. ( F ) Quantification of serum LPS in Ahr +/+ mice. ( G ) Quantification of <t>IgA</t> in Ahr +/+ mice by ELISA. Data are presented as mean ± SD; n = 5/group. NS, not significant. * p
    Iga Mouse Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Inova Diagnostics iga httg enzyme linked immunosorbent assay kit
    AHR-dependent inflammation in mice after dietary exposure to vehicle or TCDF (24 μg/kg). ( A ) qPCR analysis of inflammatory cytokine ( IL-1 β, TNF -α, IL-10, Saa1, and Saa3 ) mRNA expression in the ileum of Ahr +/+ mice. ( B ) IL-1 β and Tnf- α expression in the ileum of Ahr –/– mice. ( C ) qPCR analysis of Lcn-2 mRNA expression in the ileum of Ahr +/+ and Ahr –/– mice. ( D ) Quantification of fecal LCN2 in Ahr +/+ mice by <t>ELISA.</t> ( E ) qPCR analysis of Myosin Vb and Ptprh mRNA in the ileum of Ahr +/+ mice after TCDF treatment. ( F ) Quantification of serum LPS in Ahr +/+ mice. ( G ) Quantification of <t>IgA</t> in Ahr +/+ mice by ELISA. Data are presented as mean ± SD; n = 5/group. NS, not significant. * p
    Iga Httg Enzyme Linked Immunosorbent Assay Kit, supplied by Inova Diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam iga enzyme linked immunosorbent assay elisa kit
    Comparative titres of mucosal <t>IgA.</t> Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific <t>ELISA.</t> Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P
    Iga Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher iga enzyme linked immunosorbent assay elisa ready setgo kits
    Comparative titres of mucosal <t>IgA.</t> Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific <t>ELISA.</t> Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P
    Iga Enzyme Linked Immunosorbent Assay Elisa Ready Setgo Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Elabscience human iga immunoglobulin a elisa kit
    Boxplot <t>IgA</t> level before and after treatment.
    Human Iga Immunoglobulin A Elisa Kit, supplied by Elabscience, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam pig iga elisa kit
    Boxplot <t>IgA</t> level before and after treatment.
    Pig Iga Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Bethyl immunoglobulin a iga elisa kits
    The mean (±SEM) concentrations (mg/mL) of serum IgG, IgM, <t>IgA</t> and sIgA in piglets between maternal COS supplementation and control groups at weaning (postnatal d 19). ∗, P
    Immunoglobulin A Iga Elisa Kits, supplied by Bethyl, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Immunology Consultant Laboratory iga elisa kit
    Mesalamine increases fecal <t>IgA</t> production. ( A ) WT (n = 6 mice per group) and AhR -/- mice (n = 4 mice per group), or ( B ) bone marrow chimeric mice (BM-AhR +/+ mice and BM-AhR -/- mice) (n = 10 mice per group) were orally treated with 50 mg/kg of mesalamine for 2 weeks. Concentrations of IgA of stool samples were measured by <t>ELISA.</t> Error bars represent mean ± SEM. * P
    Iga Elisa Kit, supplied by Immunology Consultant Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mouse iga elisa kit
    Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial <t>IgA</t> and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by <t>ELISA.</t> Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p
    Mouse Iga Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam human iga elisa kit
    <t>IgA-Binding</t> of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and <t>ELISA.</t> (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p
    Human Iga Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Quant vca iga elisa kit
    <t>IgA-Binding</t> of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and <t>ELISA.</t> (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p
    Vca Iga Elisa Kit, supplied by Bio-Quant, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam rabbit iga elisa kit
    <t>IgA-Binding</t> of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and <t>ELISA.</t> (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p
    Rabbit Iga Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam abcam iga
    <t>IgA-Binding</t> of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and <t>ELISA.</t> (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p
    Abcam Iga, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bacteriophages act to induce both commensal and phage specific immunity. (A) IFN-γ measured by ELISA in the supernatant of BMDCs from MHCII −/− mice pulsed with phage for 24 hours and co-cultured with WT T cells for 72 hours. (B) IFN-γ measured by ELISA in the supernatant of BMDCs from WT mice pulsed with antigens and ovalbumin (100 μg/mL) and co-cultured with CD4+ T cells from OTII mice. (C, D) Experimental set-up of Phage-Ovalbumin co-culture experiments. IFN-γ was measured in the supernatant by ELISA. (E) Feces from bacteriophage cocktail treated germfree mice assayed for phage specific IgA by ELISA. Data are represented as mean ± SEM. *p

    Journal: Cell host & microbe

    Article Title: Expansion of bacteriophages is linked to aggravated intestinal inflammation and colitis

    doi: 10.1016/j.chom.2019.01.008

    Figure Lengend Snippet: Bacteriophages act to induce both commensal and phage specific immunity. (A) IFN-γ measured by ELISA in the supernatant of BMDCs from MHCII −/− mice pulsed with phage for 24 hours and co-cultured with WT T cells for 72 hours. (B) IFN-γ measured by ELISA in the supernatant of BMDCs from WT mice pulsed with antigens and ovalbumin (100 μg/mL) and co-cultured with CD4+ T cells from OTII mice. (C, D) Experimental set-up of Phage-Ovalbumin co-culture experiments. IFN-γ was measured in the supernatant by ELISA. (E) Feces from bacteriophage cocktail treated germfree mice assayed for phage specific IgA by ELISA. Data are represented as mean ± SEM. *p

    Article Snippet: Final supernatants were used as samples for an IgA specific ELISA kit (eBioscience).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Cell Culture, Co-Culture Assay

    PD-1 + and PD-1 Neg Tfr cells suppress antibody responses in vitro. (A) Flow cytometry plots showing the percentage of cells producing IL-10 or IL-21 within the indicated populations. All cells were first gated on live CD4 + CD45RA − T cells (not depicted), then CXCR5 and PD-1, and followed by FOXP3 and CD25. LMNCs were activated with PMA/ionomycin. Unstimulated controls were used to draw cytokine gates. Percentage of IL-10 (B) or IL-21 (C) producing cells within T cell subsets as determined by flow cytometry and gated in A. Data are pooled from three similar experiments, n = 14 subjects. (D) CD40L flow cytometry histograms. (E) Percentage of T cells that are CD40L + based on gates drawn on unstimulated controls. Data are pooled from two similar experiments, n = 10 subjects. Analysis of IgG (F) or IgA (G) secretion by ELISA after stimulation with SEB in coculture with a 3:1 ratio of Tfh cells and B cells or 3:1:1 ratio of sorted Tfh cells, PD-1 + Tfr cells, and B cells for 7 d; n = 19 subjects (F) or n = 6 subjects (G). (H) IgG secretion from Tfh cell–free coculture experiments using mitogen-activated B cells with or without PD-1 + Tfr cells at a 1:1 ratio; n = 5. (I) IgG secretion using sorted Tfh, PD-1 + Tfr cells, PD-1 Neg Tfr cells, or Treg cells at indicated ratios for 7 d n = 6–10 subjects. Not depicted: Differences between the “Tfh + B” and all other groups were highly significant. For F–I, antibody concentrations are expressed as a percentage of the corresponding “Tfh + B” group titers. Paired t tests; mean ± SEM; *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Spatial distribution and function of T follicular regulatory cells in human lymph nodes

    doi: 10.1084/jem.20171940

    Figure Lengend Snippet: PD-1 + and PD-1 Neg Tfr cells suppress antibody responses in vitro. (A) Flow cytometry plots showing the percentage of cells producing IL-10 or IL-21 within the indicated populations. All cells were first gated on live CD4 + CD45RA − T cells (not depicted), then CXCR5 and PD-1, and followed by FOXP3 and CD25. LMNCs were activated with PMA/ionomycin. Unstimulated controls were used to draw cytokine gates. Percentage of IL-10 (B) or IL-21 (C) producing cells within T cell subsets as determined by flow cytometry and gated in A. Data are pooled from three similar experiments, n = 14 subjects. (D) CD40L flow cytometry histograms. (E) Percentage of T cells that are CD40L + based on gates drawn on unstimulated controls. Data are pooled from two similar experiments, n = 10 subjects. Analysis of IgG (F) or IgA (G) secretion by ELISA after stimulation with SEB in coculture with a 3:1 ratio of Tfh cells and B cells or 3:1:1 ratio of sorted Tfh cells, PD-1 + Tfr cells, and B cells for 7 d; n = 19 subjects (F) or n = 6 subjects (G). (H) IgG secretion from Tfh cell–free coculture experiments using mitogen-activated B cells with or without PD-1 + Tfr cells at a 1:1 ratio; n = 5. (I) IgG secretion using sorted Tfh, PD-1 + Tfr cells, PD-1 Neg Tfr cells, or Treg cells at indicated ratios for 7 d n = 6–10 subjects. Not depicted: Differences between the “Tfh + B” and all other groups were highly significant. For F–I, antibody concentrations are expressed as a percentage of the corresponding “Tfh + B” group titers. Paired t tests; mean ± SEM; *, P

    Article Snippet: IgA was quantified using IgA human ELISA kits (Thermo Fisher Scientific).

    Techniques: In Vitro, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05

    Journal: PLoS ONE

    Article Title: Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets

    doi: 10.1371/journal.pone.0107508

    Figure Lengend Snippet: Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05

    Article Snippet: Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Colonized-children by Spn had higher mucosal GM of antibody levels than non-colonized children NP samples were collected from age-matched healthy children with or without Spn colonization. The rates of pneumococcal specific to total IgG and total IgA were determined by ELISA, and compared using nonparametric Mann-Whitney Test between culture positive and negative for Spn. A, anti-PhtD, anti-PcpA, and anti-PlyD1 IgG; B, anti-PhtD, anti-PcpA, and anti-PlyD1 IgA; NP, nasopharyngeal; CN: culture negative; Spn : S. pneumoniae; tIgG,tIgA, total amount of IgG, IgA. Lines represent GM with 95% CI.

    Journal: Mucosal immunology

    Article Title: Higher Levels of Mucosal Antibody to Pneumococcal Vaccine Candidate Proteins Are Associated with Reduced Acute Otitis Media Caused by Streptococcus pneumoniae in Young Children

    doi: 10.1038/mi.2015.1

    Figure Lengend Snippet: Colonized-children by Spn had higher mucosal GM of antibody levels than non-colonized children NP samples were collected from age-matched healthy children with or without Spn colonization. The rates of pneumococcal specific to total IgG and total IgA were determined by ELISA, and compared using nonparametric Mann-Whitney Test between culture positive and negative for Spn. A, anti-PhtD, anti-PcpA, and anti-PlyD1 IgG; B, anti-PhtD, anti-PcpA, and anti-PlyD1 IgA; NP, nasopharyngeal; CN: culture negative; Spn : S. pneumoniae; tIgG,tIgA, total amount of IgG, IgA. Lines represent GM with 95% CI.

    Article Snippet: To correct for differential dilution effects that occurred during the NP wash and MEF sample collection, total IgG and IgA were determined using Human IgG and IgA ELISA Quantitation Kits (Bethyl Laboratories, Montgomery, TX) according to manufacturer's protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    AHR-dependent inflammation in mice after dietary exposure to vehicle or TCDF (24 μg/kg). ( A ) qPCR analysis of inflammatory cytokine ( IL-1 β, TNF -α, IL-10, Saa1, and Saa3 ) mRNA expression in the ileum of Ahr +/+ mice. ( B ) IL-1 β and Tnf- α expression in the ileum of Ahr –/– mice. ( C ) qPCR analysis of Lcn-2 mRNA expression in the ileum of Ahr +/+ and Ahr –/– mice. ( D ) Quantification of fecal LCN2 in Ahr +/+ mice by ELISA. ( E ) qPCR analysis of Myosin Vb and Ptprh mRNA in the ileum of Ahr +/+ mice after TCDF treatment. ( F ) Quantification of serum LPS in Ahr +/+ mice. ( G ) Quantification of IgA in Ahr +/+ mice by ELISA. Data are presented as mean ± SD; n = 5/group. NS, not significant. * p

    Journal: Environmental Health Perspectives

    Article Title: Persistent Organic Pollutants Modify Gut Microbiota–Host Metabolic Homeostasis in Mice Through Aryl Hydrocarbon Receptor Activation

    doi: 10.1289/ehp.1409055

    Figure Lengend Snippet: AHR-dependent inflammation in mice after dietary exposure to vehicle or TCDF (24 μg/kg). ( A ) qPCR analysis of inflammatory cytokine ( IL-1 β, TNF -α, IL-10, Saa1, and Saa3 ) mRNA expression in the ileum of Ahr +/+ mice. ( B ) IL-1 β and Tnf- α expression in the ileum of Ahr –/– mice. ( C ) qPCR analysis of Lcn-2 mRNA expression in the ileum of Ahr +/+ and Ahr –/– mice. ( D ) Quantification of fecal LCN2 in Ahr +/+ mice by ELISA. ( E ) qPCR analysis of Myosin Vb and Ptprh mRNA in the ileum of Ahr +/+ mice after TCDF treatment. ( F ) Quantification of serum LPS in Ahr +/+ mice. ( G ) Quantification of IgA in Ahr +/+ mice by ELISA. Data are presented as mean ± SD; n = 5/group. NS, not significant. * p

    Article Snippet: We obtained IgA mouse enzyme-linked immunosorbent assay (ELISA) kits from Abcam, and Pierce LAL chromogenic endotoxin quantitation kits and lipocalin-2 (LCN2) ELISA kits from Thermo Scientific.

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay

    Comparative titres of mucosal IgA. Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific ELISA. Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P

    Journal: Frontiers in Microbiology

    Article Title: A Long-Term Efficacy Trial of a Live, Attenuated Salmonella Typhimurium Vaccine in Layer Hens

    doi: 10.3389/fmicb.2018.01380

    Figure Lengend Snippet: Comparative titres of mucosal IgA. Ileal scrapings were collected at the end of the experiment. Secreted IgA was quantified using a chicken specific ELISA. Mean sIgA titres ranged between 122.3 ± 24.9 μg IgA/mg tissue and 422.8 ± 72.6 μg IgA/mg tissue. The mean sIgA titre observed in the challenged only group ( S. Typhimurium DT9; indicated as STM9) was significantly higher than all other treatment groups ( P

    Article Snippet: Secretory IgA was measured using chicken IgA ELISA kit (Abcam ab157691).

    Techniques: Enzyme-linked Immunosorbent Assay

    Detection of IgA production using ELISA in tears (a) and tracheal wash (b) of chickens ( n = 5 per group) vaccinated with live H120 alone (group I) or in combination with CR88 (group II) at day 1. The two groups were again vaccinated with CR88 at 14 days

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Mucosal, Cellular, and Humoral Immune Responses Induced by Different Live Infectious Bronchitis Virus Vaccination Regimes and Protection Conferred against Infectious Bronchitis Virus Q1 Strain

    doi: 10.1128/CVI.00368-15

    Figure Lengend Snippet: Detection of IgA production using ELISA in tears (a) and tracheal wash (b) of chickens ( n = 5 per group) vaccinated with live H120 alone (group I) or in combination with CR88 (group II) at day 1. The two groups were again vaccinated with CR88 at 14 days

    Article Snippet: To detect IBV antibodies, serum samples were tested with a commercial IBV enzyme-linked immunosorbent assay (ELISA) kit (FlockChek; IDEXX Laboratories, Inc., Westbrook, ME, USA), and immunoglobulin A (IgA) in tears and tracheal washes was assayed using a commercial IgA chicken ELISA kit (Abcam, Cambridge, United Kingdom).

    Techniques: Enzyme-linked Immunosorbent Assay

    Boxplot IgA level before and after treatment.

    Journal: Advances in Preventive Medicine

    Article Title: Nephrolepis exaltata Herbal Mask Increases Nasal IgA Levels and Pulmonary Function in Textile Factory Workers

    doi: 10.1155/2019/5687135

    Figure Lengend Snippet: Boxplot IgA level before and after treatment.

    Article Snippet: After the above procedure, samples were assessed using ELISA (Enzyme-Linked Immunosorbent Assay) methodology with Human IgA (Immunoglobulin A) ELISA Kit from Elabscience® (catalog number E-EL-H1355).

    Techniques:

    Mean of delta score of IgA level between two groups.

    Journal: Advances in Preventive Medicine

    Article Title: Nephrolepis exaltata Herbal Mask Increases Nasal IgA Levels and Pulmonary Function in Textile Factory Workers

    doi: 10.1155/2019/5687135

    Figure Lengend Snippet: Mean of delta score of IgA level between two groups.

    Article Snippet: After the above procedure, samples were assessed using ELISA (Enzyme-Linked Immunosorbent Assay) methodology with Human IgA (Immunoglobulin A) ELISA Kit from Elabscience® (catalog number E-EL-H1355).

    Techniques:

    The mean (±SEM) concentrations (mg/mL) of serum IgG, IgM, IgA and sIgA in piglets between maternal COS supplementation and control groups at weaning (postnatal d 19). ∗, P

    Journal: Animal Nutrition

    Article Title: Maternal chitosan oligosaccharide intervention optimizes the production performance and health status of gilts and their offspring

    doi: 10.1016/j.aninu.2020.02.001

    Figure Lengend Snippet: The mean (±SEM) concentrations (mg/mL) of serum IgG, IgM, IgA and sIgA in piglets between maternal COS supplementation and control groups at weaning (postnatal d 19). ∗, P

    Article Snippet: A pig immunoglobulin M (IgM) ELISA kit (Life Diagnostics, Inc. USA, Catalog no. 5015-9), immunoglobulin G (IgG) and immunoglobulin A (IgA) ELISA kits (Bethyl Laboratories, Inc. USA, Catalog no. E101-104 and Catalog no. E100-102) were used to determine the serum concentrations of IgM, IgG, and IgA, respectively.

    Techniques:

    Expression levels of IgM, IgG, IgA, sIgA in serum and sIgA in faecal samples of the COS treatment and control gilts at 19 d after giving birth and 90 d of gestation, respectively. (A) The mean (±SEM) concentrations (mg/mL) of immune markers of IgM, IgG, IgA and sIgA in blood serum samples of the COS treatment and control gilts at weaning d 19 after giving birth. Samples without any hemolysis were used in this assay. (B) The mean (±SEM) of sIgA concentration (mg/mL) in faecal samples of the COS treatment and control gilts at d 90 of gestation. ∗, P

    Journal: Animal Nutrition

    Article Title: Maternal chitosan oligosaccharide intervention optimizes the production performance and health status of gilts and their offspring

    doi: 10.1016/j.aninu.2020.02.001

    Figure Lengend Snippet: Expression levels of IgM, IgG, IgA, sIgA in serum and sIgA in faecal samples of the COS treatment and control gilts at 19 d after giving birth and 90 d of gestation, respectively. (A) The mean (±SEM) concentrations (mg/mL) of immune markers of IgM, IgG, IgA and sIgA in blood serum samples of the COS treatment and control gilts at weaning d 19 after giving birth. Samples without any hemolysis were used in this assay. (B) The mean (±SEM) of sIgA concentration (mg/mL) in faecal samples of the COS treatment and control gilts at d 90 of gestation. ∗, P

    Article Snippet: A pig immunoglobulin M (IgM) ELISA kit (Life Diagnostics, Inc. USA, Catalog no. 5015-9), immunoglobulin G (IgG) and immunoglobulin A (IgA) ELISA kits (Bethyl Laboratories, Inc. USA, Catalog no. E101-104 and Catalog no. E100-102) were used to determine the serum concentrations of IgM, IgG, and IgA, respectively.

    Techniques: Expressing, Concentration Assay

    The ELISA of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native IgA upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.

    Journal: PLoS ONE

    Article Title: Suppression of Adiponectin by Aberrantly Glycosylated IgA1 in Glomerular Mesangial Cells In Vitro and In Vivo

    doi: 10.1371/journal.pone.0033965

    Figure Lengend Snippet: The ELISA of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native IgA upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.

    Article Snippet: The samples, including non-treated IgA (native IgA) and neuraminidase- and galactosidase-treated IgA, were added to each well, incubated overnight, and diluted to achieve comparable levels of IgA (50 µg/ml) as determined using the IgA ELISA kit (Bethyl Laboratories, Montgomery, TX, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Molecular Weight, Concentration Assay, Cell Culture

    Mesalamine increases fecal IgA production. ( A ) WT (n = 6 mice per group) and AhR -/- mice (n = 4 mice per group), or ( B ) bone marrow chimeric mice (BM-AhR +/+ mice and BM-AhR -/- mice) (n = 10 mice per group) were orally treated with 50 mg/kg of mesalamine for 2 weeks. Concentrations of IgA of stool samples were measured by ELISA. Error bars represent mean ± SEM. * P

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Induction of Colonic Regulatory T Cells by Mesalamine by Activating the Aryl Hydrocarbon Receptor

    doi: 10.1016/j.jcmgh.2017.03.010

    Figure Lengend Snippet: Mesalamine increases fecal IgA production. ( A ) WT (n = 6 mice per group) and AhR -/- mice (n = 4 mice per group), or ( B ) bone marrow chimeric mice (BM-AhR +/+ mice and BM-AhR -/- mice) (n = 10 mice per group) were orally treated with 50 mg/kg of mesalamine for 2 weeks. Concentrations of IgA of stool samples were measured by ELISA. Error bars represent mean ± SEM. * P

    Article Snippet: After centrifugation (4,000 × g , 20 minutes), supernatants were collected and IgA concentrations were measured using the IgA ELISA kit (ICL Lab, Portland, OR).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial IgA and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by ELISA. Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p

    Journal: Journal of diabetes and its complications

    Article Title: Endothelial Cell-Specific Overexpression of Endothelial Nitric Oxide Synthase in Ins2Akita Mice Exacerbates Diabetic Nephropathy

    doi: 10.1016/j.jdiacomp.2018.10.003

    Figure Lengend Snippet: Immunofluorescence studies of glomeruli (A) A panel of immunofluorescent stains was performed on frozen kidney sections of WT, AK, eNOSTg and eNOSTg/AK mice. WT glomeruli showed nonspecific staining with IgM, consistent with entrapment (data not shown). AK, eNOSTg and eNOSTg/AK glomeruli showed mesangial IgA and C3 staining along with some mesangial IgM staining. These findings correlated with the presence of mesangial deposits on electron microscopy. Representative images are shown. (n = 3-4 mice/group) Original magnification: x400, (B) Serum IgA analysis by ELISA. Compared with WT, eNOSTg/AK mice showed a 3-fold increase in circulating IgA levels. In contrast, eNOSTg mice showed IgA levels comparable to WT mice. Data represents mean (SEM), **p

    Article Snippet: IgA levels were determined using a mouse IgA ELISA kit purchased from Thermoscientific (Frederick, MD).

    Techniques: Immunofluorescence, Mouse Assay, Staining, Electron Microscopy, Enzyme-linked Immunosorbent Assay

    Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse IgA or anti-IgM antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p

    Journal: Scientific Reports

    Article Title: Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance

    doi: 10.1038/s41598-017-05212-4

    Figure Lengend Snippet: Increased pneumococcal binding capacities by lung mucosal fluid in inflamed lungs. Pneumococci were co-incubated with bronchoalveolar lavage fluid (BALF) supernatants from SPC-HA and SPC-HAxTCR-HA mice. Bacteria were stained with anti-mouse IgA or anti-IgM antibodies and analyzed by flow cytometry (FACS). ( a ) Representative FACS plots of IgA+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgA without prior incubation with BALF. ( b ) Percentages of IgA+ pneumococci and relative fluorescence intensities ( c ) of IgA+ pneumococci. ( d ) Representative FACS plots of IgM+ pneumococci incubated with BALF from SPC-HA or SPC-HAxTCR-mice; control samples (CTRL) were stained with anti-IgM without prior incubation with BALF. ( e ) Percentages of IgM+ pneumococci and relative fluorescence intensities ( f ) of IgM+ pneumococci. Relative fluorescence intensities are calculated by the ratio of the MFI of each individual sample over the mean MFI of the SPC-HA control group. Data are pooled from 2 independent experiments with similar results. *p

    Article Snippet: ELISA ELISA kits for mouse IgA and IgM (eBioscience, Frankfurt am Main, Germany), mouse IL-6 and TNF-α (BioLegend, London, United Kingdom), mouse C5a (R & D Systems, Minneapolis, MN) and mouse albumin (Bethyl Laboratories Inc., Montogomery, TX) were used according to manufacturer’s instructions.

    Techniques: Binding Assay, Incubation, Mouse Assay, Staining, Flow Cytometry, Cytometry, FACS, Fluorescence

    Increased mucosal transport of secretory antibodies in inflamed lungs. ( a ) anti-pIgR and anti-Gapdh immunoblot of 20 µg protein from whole lung homogenates of SPC-HA (n = 3) and SPC-HAxTCR-HA (n = 3) mice. Densitometric quantification of protein bands is stated in arbitrary units above each lane. Relative pIgR quantity was calculated normalizing densitometric pIgR value to the corresponding Gapdh value and subsequently comparing normalized pIgR values of the SPC-HAxTCR-HA group to the SPC-HA group. Data are representative for at least two individual experiments with similar results. ( b ) Lung tissue sections were stained with anti-pIgR (green), representative alveolar structures from n = 3/group are depicted. White circles illustrate representative densitometrically quantified tissue areas. Calculated total cell fluorescence (CTCF) was determined as: Integrated density of fluorescence-positive cell – (Area of fluorescence-positive cell × mean fluorescence intensity of background signal). Median CTCF of quantified areas in representative images are depicted as white numbers. IgA and IgM levels in ( c ) bronchoalveolar lavage fluid (BALF) and ( d ) serum of SPC-HA and SPC-HAxTCR-HA mice were determined by ELISA. ( e ) Relative secretory IgA concentrations in serial dilutions of BALF samples were determined by ELISA. Results are expressed as the mean optical density (OD) at 450 nm ± SEM, *p

    Journal: Scientific Reports

    Article Title: Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance

    doi: 10.1038/s41598-017-05212-4

    Figure Lengend Snippet: Increased mucosal transport of secretory antibodies in inflamed lungs. ( a ) anti-pIgR and anti-Gapdh immunoblot of 20 µg protein from whole lung homogenates of SPC-HA (n = 3) and SPC-HAxTCR-HA (n = 3) mice. Densitometric quantification of protein bands is stated in arbitrary units above each lane. Relative pIgR quantity was calculated normalizing densitometric pIgR value to the corresponding Gapdh value and subsequently comparing normalized pIgR values of the SPC-HAxTCR-HA group to the SPC-HA group. Data are representative for at least two individual experiments with similar results. ( b ) Lung tissue sections were stained with anti-pIgR (green), representative alveolar structures from n = 3/group are depicted. White circles illustrate representative densitometrically quantified tissue areas. Calculated total cell fluorescence (CTCF) was determined as: Integrated density of fluorescence-positive cell – (Area of fluorescence-positive cell × mean fluorescence intensity of background signal). Median CTCF of quantified areas in representative images are depicted as white numbers. IgA and IgM levels in ( c ) bronchoalveolar lavage fluid (BALF) and ( d ) serum of SPC-HA and SPC-HAxTCR-HA mice were determined by ELISA. ( e ) Relative secretory IgA concentrations in serial dilutions of BALF samples were determined by ELISA. Results are expressed as the mean optical density (OD) at 450 nm ± SEM, *p

    Article Snippet: ELISA ELISA kits for mouse IgA and IgM (eBioscience, Frankfurt am Main, Germany), mouse IL-6 and TNF-α (BioLegend, London, United Kingdom), mouse C5a (R & D Systems, Minneapolis, MN) and mouse albumin (Bethyl Laboratories Inc., Montogomery, TX) were used according to manufacturer’s instructions.

    Techniques: Mouse Assay, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay

    IgA-Binding of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and ELISA. (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p

    Journal: Cell

    Article Title: Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10

    doi: 10.1016/j.cell.2018.11.035

    Figure Lengend Snippet: IgA-Binding of Bacteria in the Gut Is Reduced in MS Patients during an Acute Relapse (A) Study overview: fresh-frozen fecal samples of 33 patients with clinically Isolated syndrome (CIS) or relapslng-remlttlng multiple sclerosis (RRMS) (n = 22 during disease remission, n = 11 during an acute relapse) and 32 healthy controls were collected. Fecal bacteria were isolated and IgA-binding of autologous gut bacteria was quantified by bacterial flow cytometry (BUGFlow) and ELISA. (B) BUGFlow: bacteria were identified based on forward and side scatter and the IgA-positive population was defined based on an isotype control. Shown is an example of staining of fecal bacteria from an RRMS patient with an isotype control (left) as well as with anti-IgA during remission (middle) and during relapse (right). (C) Percentage of fecal-bound IgA by BUG Flow: 1 × 10 6 fecal bacteria were stained with anti-IgA and % of IgA binding was assessed by flow cytometry. Values shown are replicates from 2 experiments. (D) Quantification of fecal-bound IgA by ELISA: 1 × 10 6 fecal bacteria were assessed for IgA binding using a commercially available quantitative IgA ELISA assay. Values shown are mean values of duplicate measurements. *p

    Article Snippet: One million isolated fecal bacteria per individual were coated in duplicates and probed for IgA using a commercially available quantitative ELISA Assay (Human IgA ELISA Kit, Abcam).

    Techniques: Binding Assay, Mass Spectrometry, Isolation, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Staining

    Thymic stromal lymphopoietin (TSLP) enhanced IgA production in follicular dendritic cell (FDC)-associated clusters through TSLP receptor (TSLPR), and serum IgA and TSLP concentrations (A) Left to right in the upper row: morphology, CD10 + GC cells and CD21 + FDCs in FDC-associated clusters isolated from tonsillar GCs of IgAN patients. FDC-associated clusters are composed of CD 10 + GC cells and CD21 + FDCs, with about 1 FDC per 10 lymphocytes in each FDC-associated cluster. (B, C) Left to right in the lower row: IgA and TSLP concentrations in the supernatants of FDC-associated clusters. IgA and TSLP were quantified in the supernatants using ELISA. Combined data (mean ± SD) from experiments using FDC-associated clusters from 3 IgAN patients are presented. (D, E) The IgA and TSLP concentrations in the sera of 20 IgAN patients with Tripterygium Wilfordii treatment, 20 IgAN patients without treatment, 20 non-IgAN patients with chronic tonsillitis patients, and 20 healthy volunteers were measured by ELISA. Error bars show means ± SEMs. * , P

    Journal: Oncotarget

    Article Title: Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy

    doi: 10.18632/oncotarget.22561

    Figure Lengend Snippet: Thymic stromal lymphopoietin (TSLP) enhanced IgA production in follicular dendritic cell (FDC)-associated clusters through TSLP receptor (TSLPR), and serum IgA and TSLP concentrations (A) Left to right in the upper row: morphology, CD10 + GC cells and CD21 + FDCs in FDC-associated clusters isolated from tonsillar GCs of IgAN patients. FDC-associated clusters are composed of CD 10 + GC cells and CD21 + FDCs, with about 1 FDC per 10 lymphocytes in each FDC-associated cluster. (B, C) Left to right in the lower row: IgA and TSLP concentrations in the supernatants of FDC-associated clusters. IgA and TSLP were quantified in the supernatants using ELISA. Combined data (mean ± SD) from experiments using FDC-associated clusters from 3 IgAN patients are presented. (D, E) The IgA and TSLP concentrations in the sera of 20 IgAN patients with Tripterygium Wilfordii treatment, 20 IgAN patients without treatment, 20 non-IgAN patients with chronic tonsillitis patients, and 20 healthy volunteers were measured by ELISA. Error bars show means ± SEMs. * , P

    Article Snippet: After collection of supernatants, qualitative detection of TSLP and IgA in cell supernatants was performed using Human TSLP ELISA MAX Deluxe Sets (Biolegend, San Diego, CA, USA) and IgA Human ELISA Kit (ab137980; Abcam).

    Techniques: Isolation, Enzyme-linked Immunosorbent Assay