Journal: Nature Communications
Article Title: Deciphering the importance of the palindromic architecture of the immunoglobulin heavy-chain 3' regulatory region
Figure Lengend Snippet: Influence of the 3'RR palindrome on Ig synthesis. ( a ) ELISA analysis of IgG 1 , IgG 2a , IgG 2b , IgG 3 and IgA in supernatants of LPS±IL-4-, INFγ- and TGFβ-stimulated splenocytes of ΔleftPAL, Δ IRIS and wt mice. Data are the mean±s.e.m. of eight experiments with one mouse (8–12 weeks old, male and female). * P
Article Snippet: Culture supernatants and plasma (first dilution to 1:50) from ΔleftPAL, ΔIRIS and wt mice were analysed for the presence of the various Ig classes (IgM, IgG1 , IgG2b , IgG2b , IgG3 and IgA) by ELISA, as previously described above , except for the coating made with suitable capture antibodies (2 μg ml−1 for IgM (Southern Biotechnologies, ref: 902001), IgG1 (Southern Biotechnologies, ref: 107001), IgG2a (Southern Biotechnologies, ref: 731956) and IgG2b (Southern Biotechnologies, ref: 731940), 3 μg ml−1 for IgG3 (Southern Biotechnologies, ref: 732371) and 4 μg ml−1 for IgA (Southern Biotechnologies, ref: 104001), blocking performed with 3% BSA in PBS, AP-conjugated goat antisera specific for mouse IgA (Southern Biotechnologies, ref: 104004) and IgG3 (Beckman Coulter, ref: 732374), and for standards made with specific antisera for IgM, IgG1 , IgG2a , IgG2b , IgG3 and IgA (Southern Biotechnologies)).
Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay