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  • 99
    Thermo Fisher polymeric iga2 antibodies
    Schematic representation of the molecular forms of human <t>IgA</t>
    Polymeric Iga2 Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore iga
    Second-site immunization in the presence of PRO 2000 increases systemic and mucosal antibody responses. BALB/c mice (n = 7) were primed intranasally with 10 µg gp140 per animal in polycation and were boosted three times vaginally with gp140 +/− PRO 2000. Samples were collected 14 days after the final vaginal immunization. Serum <t>IgG</t> endpoint titres ( A ), or vaginal lavage IgG ( B ) and <t>IgA</t> ( C ) endpoint titres normalized to the total IgG or IgA content or each sample, respectively, are displayed. gp140 + PRO , combination of gp140 and PRO 2000.* p
    Iga, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2032 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson iga1 iga2
    Immature naïve B cells, IgM levels and CD4:CD8 ratios show differences between AGA and SGA cord blood. (A) Comparison of proportions of B cell subsets and absolute concentrations (median, IQR and 95% CI) along with p values. (B) Comparison of absolute concentrations of IgM and <t>IgA</t> in cord blood (median, IQR and 95% CI) along with p values. (C) Comparison of proportions of T cell subsets (median, IQR and 95% CI) along with p values. (D) Comparison of absolute numbers of T cell subsets (median, IQR and 95% CI) along with p values. (E) Comparison of CD4:CD8 ratios (median, IQR and 95% CI) along with p value.
    Iga1 Iga2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Nordic-Mubio iga2
    Arithmetic means (± standard deviations) of the percentages of IgA1- and <t>IgA2-secreting</t> cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01
    Iga2, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 90/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Synthesis Inc immunoglobulin a iga polyclonal antibodies
    Arithmetic means (± standard deviations) of the percentages of IgA1- and <t>IgA2-secreting</t> cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01
    Immunoglobulin A Iga Polyclonal Antibodies, supplied by Bio-Synthesis Inc, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    iga1  (Abcam)
    91
    Abcam iga1
    Glomerular deposition of galactose-deficient <t>IgA1</t> in patients with types of secondary IgA nephropathy (IgANs) and controls. Double staining with anti−Gd-IgA1 mAb (red) and anti-IgA1 mAb (green). Merged figures were also added to the 4′,6-diamidino-2-phenylindole channel. Bars = 50 μm.
    Iga1, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    SouthernBiotech iga
    Influence of the 3'RR palindrome on Ig synthesis. ( a ) ELISA analysis of IgG 1 , IgG 2a , IgG 2b , IgG 3 and <t>IgA</t> in supernatants of LPS±IL-4-, INFγ- and TGFβ-stimulated splenocytes of ΔleftPAL, Δ IRIS and wt mice. Data are the mean±s.e.m. of eight experiments with one mouse (8–12 weeks old, male and female). * P
    Iga, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 2053 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Accurate Chemical & Scientific Corporation iga2
    Serum <t>IgA1</t> (▴) and IgG (⧫) anti-C-GTF responses after i.n. immunization with liposomal C-GTF. Values are the percent increase in anti-C-GTF activity in serum samples (over three preimmunization samples) collected from human subjects after i.n. immunization with C-GTF (combined data from liposomal C-GTF and free C-GTF groups).
    Iga2, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam anti iga antibody
    Serum <t>IgA1</t> (▴) and IgG (⧫) anti-C-GTF responses after i.n. immunization with liposomal C-GTF. Values are the percent increase in anti-C-GTF activity in serum samples (over three preimmunization samples) collected from human subjects after i.n. immunization with C-GTF (combined data from liposomal C-GTF and free C-GTF groups).
    Anti Iga Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Siemens Healthineers immunoglobulin a
    Serum <t>IgA1</t> (▴) and IgG (⧫) anti-C-GTF responses after i.n. immunization with liposomal C-GTF. Values are the percent increase in anti-C-GTF activity in serum samples (over three preimmunization samples) collected from human subjects after i.n. immunization with C-GTF (combined data from liposomal C-GTF and free C-GTF groups).
    Immunoglobulin A, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher iga
    The representative figure showed that SO 2 inhalation did not affect <t>IgG</t> levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : <t>IgA</t> level in the serum; f : IgA level in the nasal septum
    Iga, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore iga2 nordic
    Arithmetic means (± standard deviations) of the percentages of IgA1- and <t>IgA2-secreting</t> cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01
    Iga2 Nordic, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bethyl immunoglobulin a iga assays
    Arithmetic means (± standard deviations) of the percentages of IgA1- and <t>IgA2-secreting</t> cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01
    Immunoglobulin A Iga Assays, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    InvivoGen control iga1
    J8 and S2-specific antibody response and spleen cell proliferative response to antigens for individual BALB/c mice (n = 5/group). Mean antibody titer + SEM are shown. ( A ) J8-specific salivary <t>IgA</t> antibody response for BALB/c mice (n = 5/group). Mean antibody titers are represented as a bar + SEM. ( B ) S2-specific salivary IgA titer. ( C ) Average stimulation index (SI) of spleen cell proliferation for BALB/c mice immunized with 30 μg of S2 peptide and 30 μg of DT in complete Freund’s adjuvant. Proliferation of cells induced by S2 peptide and DT (30 μg) and concanavalin A (conA) was determined. The average counts per minute observed for control mice in the absence of antigen were 5303 cpm. ( D ) S2-specific serum <t>IgG</t> titer. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test to compare test groups to the PBS control group (ns, p > 0.05; * p
    Control Iga1, supplied by InvivoGen, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson biotinylated mouse anti human iga1 iga2
    Breast milk <t>IgA</t> levels specific to casein (A) and beta-lactoglobulin (BLG) (B) in infants with no cow’s milk allergy (CMA) and in those with either immediate-type or delayed-type CMA. On the box plots the median is represented by a horizontal line within the box representing the 25 th to 75 th percentile, whiskers show the 5 th to 95 th percentile and the square symbol is the geometric mean.
    Biotinylated Mouse Anti Human Iga1 Iga2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    IGAN Bio immunoglobulin a iga nephropathy
    A. Saturation isotherm and Scatchard analysis of 125 I-UG binding to Fn . Binding isotherms of hUG to Fn were performed by incubating increasing concentrations of 125 I-UG. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of hUG, from the total binding (■). The data shown are the mean of triplicate measurements±SD. B. Scatchard plots of hUG binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 15 nM ± 2.6 nM (n = 3). C. Binding isotherms of <t>IgA</t> to Fn. Binding isotherms of IgA to Fn were performed by incubating increasing concentration of 125 I-IgA. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of IgA, from the total binding (■). The data shown are the mean of triplicate measurements. D. Scatchard plots of IgA binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 65 nM ± 5.2 nM (n = 3).
    Immunoglobulin A Iga Nephropathy, supplied by IGAN Bio, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam anti iga
    A. Saturation isotherm and Scatchard analysis of 125 I-UG binding to Fn . Binding isotherms of hUG to Fn were performed by incubating increasing concentrations of 125 I-UG. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of hUG, from the total binding (■). The data shown are the mean of triplicate measurements±SD. B. Scatchard plots of hUG binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 15 nM ± 2.6 nM (n = 3). C. Binding isotherms of <t>IgA</t> to Fn. Binding isotherms of IgA to Fn were performed by incubating increasing concentration of 125 I-IgA. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of IgA, from the total binding (■). The data shown are the mean of triplicate measurements. D. Scatchard plots of IgA binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 65 nM ± 5.2 nM (n = 3).
    Anti Iga, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Bethyl immunoglobulin a iga antibodies
    A. Saturation isotherm and Scatchard analysis of 125 I-UG binding to Fn . Binding isotherms of hUG to Fn were performed by incubating increasing concentrations of 125 I-UG. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of hUG, from the total binding (■). The data shown are the mean of triplicate measurements±SD. B. Scatchard plots of hUG binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 15 nM ± 2.6 nM (n = 3). C. Binding isotherms of <t>IgA</t> to Fn. Binding isotherms of IgA to Fn were performed by incubating increasing concentration of 125 I-IgA. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of IgA, from the total binding (■). The data shown are the mean of triplicate measurements. D. Scatchard plots of IgA binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 65 nM ± 5.2 nM (n = 3).
    Immunoglobulin A Iga Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    ALPCO immunoglobulin a sciga
    Normalized matricellular proteins and risk of developing BPD or death. Normalized SPARC levels were significantly higher in babies developing BPD or death prior to 36 weeks postmenstrual age (*p = 0.0185, Mann Whitney U test). There were no other significant differences in normalized matricellular protein concentration between infants who did not develop BPD and infants who developed BPD or died prior to 36 weeks postmenstrual age. Tracheal aspirate matricellular protein and secretory component of <t>IgA</t> <t>(scIgA)</t> concentrations were measured by ELISA or multiplex immune assay. The data show individual matricellular protein levels normalized to scIgA concentration. Medians and interquartile ranges are shown.
    Immunoglobulin A Sciga, supplied by ALPCO, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    SouthernBiotech iga antibody
    Total influenza‐specific systemic and mucosal <t>IgG</t> and <t>IgA</t> antibodies. ELISA plates were coated with vaccine (H5N1) or challenge (H1N1) virus. Titers of influenza‐specific IgG and IgA antibodies against both viruses were measured in pre‐challenge sera and vaginal washes obtained from mice vaccinated as in Figure 1 . As a positive control, pooled sera obtained from mice primed intraperitoneally with live PR 8 (H1N1) virus were used. Experiments were performed twice. Bars represent mean antibody titers ± SEM of six mice per group. * * P
    Iga Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 89/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Elabscience human iga immunoglobulin a elisa kit
    Boxplot <t>IgA</t> level before and after treatment.
    Human Iga Immunoglobulin A Elisa Kit, supplied by Elabscience, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Kent Laboratories human myeloma serum immunoglobulin a iga
    The rate of G. vaginalis sialic acid (Neu5Ac) consumption from sialoglycans is substrate-dependent and inhibited by N -glycolylneuraminic acid (Neu5Gc). Washed G. vaginalis JCP8151B was incubated with 0.2 mg/ml of human <t>IgA</t> or 0.3 mg/ml of BSM, followed
    Human Myeloma Serum Immunoglobulin A Iga, supplied by Kent Laboratories, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Becton Dickinson biotinylated mouse anti iga1 iga2 mab
    The rate of G. vaginalis sialic acid (Neu5Ac) consumption from sialoglycans is substrate-dependent and inhibited by N -glycolylneuraminic acid (Neu5Gc). Washed G. vaginalis JCP8151B was incubated with 0.2 mg/ml of human <t>IgA</t> or 0.3 mg/ml of BSM, followed
    Biotinylated Mouse Anti Iga1 Iga2 Mab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic representation of the molecular forms of human IgA

    Journal: Methods (San Diego, Calif.)

    Article Title: Simplifying the synthesis of SIgA: combination of dIgA and rhSC using affinity chromatography

    doi: 10.1016/j.ymeth.2013.06.022

    Figure Lengend Snippet: Schematic representation of the molecular forms of human IgA

    Article Snippet: Briefly, supernatant from IgA2 b12/J-chain producing CHO cells [ ] was pooled and sterile filtered before polymeric IgA2 antibodies (pIgA2 b12) were purified by Protein L affinity chromatography (Thermo Fisher Scientific, Rockford, IL).

    Techniques:

    Second-site immunization in the presence of PRO 2000 increases systemic and mucosal antibody responses. BALB/c mice (n = 7) were primed intranasally with 10 µg gp140 per animal in polycation and were boosted three times vaginally with gp140 +/− PRO 2000. Samples were collected 14 days after the final vaginal immunization. Serum IgG endpoint titres ( A ), or vaginal lavage IgG ( B ) and IgA ( C ) endpoint titres normalized to the total IgG or IgA content or each sample, respectively, are displayed. gp140 + PRO , combination of gp140 and PRO 2000.* p

    Journal: PLoS ONE

    Article Title: A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment

    doi: 10.1371/journal.pone.0015861

    Figure Lengend Snippet: Second-site immunization in the presence of PRO 2000 increases systemic and mucosal antibody responses. BALB/c mice (n = 7) were primed intranasally with 10 µg gp140 per animal in polycation and were boosted three times vaginally with gp140 +/− PRO 2000. Samples were collected 14 days after the final vaginal immunization. Serum IgG endpoint titres ( A ), or vaginal lavage IgG ( B ) and IgA ( C ) endpoint titres normalized to the total IgG or IgA content or each sample, respectively, are displayed. gp140 + PRO , combination of gp140 and PRO 2000.* p

    Article Snippet: Total Ig determination by multiplex immunoassay The total IgG and IgA concentration of mouse vaginal lavage was determined using a multiplex mouse immunoglobulin isotyping kit (Millipore, Billerica, MA) following the manufacturer's instructions.

    Techniques: Mouse Assay

    PRO 2000 co-application induces increased systemic and mucosal antibody responses in rabbits. New Zealand White rabbits (n = 6) received six vaginal immunizations using 50 mg antigen per dose in the presence or absence of 1% PRO 2000. ( A ) Time course of rabbit serum IgG endpoint titres. ( B ) Endpoint IgG titres of week 12 vaginal lavage samples. ( C ) Endpoint IgA titres of week 12 vaginal lavage samples. ( D ) MW965 pseudovirus neutralization of week 12 vaginal lavage samples. Horizontal bars represent geometric means. Samples were tested at 1∶8 dilution; 100% infection level was defined by pre-immune samples. ( E ) Correlation of the rabbit serum- and vaginal gp140-specific IgG responses with the MW965 pseudovirus neutralization ( D ) of week 12 samples. Red symbols represent data from animals immunized with gp140 + PRO 2000. Pre-immune indicates samples collected prior to the first immunization; gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p

    Journal: PLoS ONE

    Article Title: A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment

    doi: 10.1371/journal.pone.0015861

    Figure Lengend Snippet: PRO 2000 co-application induces increased systemic and mucosal antibody responses in rabbits. New Zealand White rabbits (n = 6) received six vaginal immunizations using 50 mg antigen per dose in the presence or absence of 1% PRO 2000. ( A ) Time course of rabbit serum IgG endpoint titres. ( B ) Endpoint IgG titres of week 12 vaginal lavage samples. ( C ) Endpoint IgA titres of week 12 vaginal lavage samples. ( D ) MW965 pseudovirus neutralization of week 12 vaginal lavage samples. Horizontal bars represent geometric means. Samples were tested at 1∶8 dilution; 100% infection level was defined by pre-immune samples. ( E ) Correlation of the rabbit serum- and vaginal gp140-specific IgG responses with the MW965 pseudovirus neutralization ( D ) of week 12 samples. Red symbols represent data from animals immunized with gp140 + PRO 2000. Pre-immune indicates samples collected prior to the first immunization; gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p

    Article Snippet: Total Ig determination by multiplex immunoassay The total IgG and IgA concentration of mouse vaginal lavage was determined using a multiplex mouse immunoglobulin isotyping kit (Millipore, Billerica, MA) following the manufacturer's instructions.

    Techniques: Neutralization, Infection, Transformation Assay, Enzyme-linked Immunosorbent Assay

    PRO 2000 co-application induces increased mucosal antibody responses in mice. BALB/c mice (n = 7) were immunized four times vaginally with 12.5 µg gp140 +/− 1% PRO 2000; Time courses of the vaginal lavage IgA ( A ) and IgG ( B ) endpoint titres. gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p

    Journal: PLoS ONE

    Article Title: A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment

    doi: 10.1371/journal.pone.0015861

    Figure Lengend Snippet: PRO 2000 co-application induces increased mucosal antibody responses in mice. BALB/c mice (n = 7) were immunized four times vaginally with 12.5 µg gp140 +/− 1% PRO 2000; Time courses of the vaginal lavage IgA ( A ) and IgG ( B ) endpoint titres. gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p

    Article Snippet: Total Ig determination by multiplex immunoassay The total IgG and IgA concentration of mouse vaginal lavage was determined using a multiplex mouse immunoglobulin isotyping kit (Millipore, Billerica, MA) following the manufacturer's instructions.

    Techniques: Mouse Assay, Transformation Assay, Enzyme-linked Immunosorbent Assay

    Immature naïve B cells, IgM levels and CD4:CD8 ratios show differences between AGA and SGA cord blood. (A) Comparison of proportions of B cell subsets and absolute concentrations (median, IQR and 95% CI) along with p values. (B) Comparison of absolute concentrations of IgM and IgA in cord blood (median, IQR and 95% CI) along with p values. (C) Comparison of proportions of T cell subsets (median, IQR and 95% CI) along with p values. (D) Comparison of absolute numbers of T cell subsets (median, IQR and 95% CI) along with p values. (E) Comparison of CD4:CD8 ratios (median, IQR and 95% CI) along with p value.

    Journal: PLoS ONE

    Article Title: Underweight Full-Term Indian Neonates Show Differences in Umbilical Cord Blood Leukocyte Phenotype: A Cross-Sectional Study

    doi: 10.1371/journal.pone.0123589

    Figure Lengend Snippet: Immature naïve B cells, IgM levels and CD4:CD8 ratios show differences between AGA and SGA cord blood. (A) Comparison of proportions of B cell subsets and absolute concentrations (median, IQR and 95% CI) along with p values. (B) Comparison of absolute concentrations of IgM and IgA in cord blood (median, IQR and 95% CI) along with p values. (C) Comparison of proportions of T cell subsets (median, IQR and 95% CI) along with p values. (D) Comparison of absolute numbers of T cell subsets (median, IQR and 95% CI) along with p values. (E) Comparison of CD4:CD8 ratios (median, IQR and 95% CI) along with p value.

    Article Snippet: Serum IgM and IgA enzyme-linked immunosorbent assays (ELISAs) Serum IgM and IgA levels were determined at THSTI using standard sandwich ELISA assays for IgM and IgA1/IgA2 (BD Biosciences, San Jose, CA, and Southern Biotech, Birmingham, AL).

    Techniques:

    Arithmetic means (± standard deviations) of the percentages of IgA1- and IgA2-secreting cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Expression of Homing Receptors on IgA1 and IgA2 Plasmablasts in Blood Reflects Differential Distribution of IgA1 and IgA2 in Various Body Fluids ▿

    doi: 10.1128/CVI.00475-09

    Figure Lengend Snippet: Arithmetic means (± standard deviations) of the percentages of IgA1- and IgA2-secreting cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01

    Article Snippet: Salmonella serovar Typhi-specific IgA1 and IgA2 were found in all secretions, consistent with our previous results for Salmonella serovar Typhi-specific total IgA ( ).

    Techniques: Expressing

    Arithmetic means (± standard deviations) of the percentages of antigen-specific IgA1- and IgA2-secreting cells expressing the indicated HR in healthy volunteers after oral vaccination with live attenuated Salmonella serovar Typhi Ty21a (A) or parenteral vaccination with an unconjugated pneumococcal polysaccharide vaccine (B). The numbers of volunteers from whom the data were pooled are given under the bars. There were no statistically significant differences between IgA1 and IgA2 ASC in the paired Student's t test.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Expression of Homing Receptors on IgA1 and IgA2 Plasmablasts in Blood Reflects Differential Distribution of IgA1 and IgA2 in Various Body Fluids ▿

    doi: 10.1128/CVI.00475-09

    Figure Lengend Snippet: Arithmetic means (± standard deviations) of the percentages of antigen-specific IgA1- and IgA2-secreting cells expressing the indicated HR in healthy volunteers after oral vaccination with live attenuated Salmonella serovar Typhi Ty21a (A) or parenteral vaccination with an unconjugated pneumococcal polysaccharide vaccine (B). The numbers of volunteers from whom the data were pooled are given under the bars. There were no statistically significant differences between IgA1 and IgA2 ASC in the paired Student's t test.

    Article Snippet: Salmonella serovar Typhi-specific IgA1 and IgA2 were found in all secretions, consistent with our previous results for Salmonella serovar Typhi-specific total IgA ( ).

    Techniques: Expressing

    Glomerular deposition of galactose-deficient IgA1 in patients with types of secondary IgA nephropathy (IgANs) and controls. Double staining with anti−Gd-IgA1 mAb (red) and anti-IgA1 mAb (green). Merged figures were also added to the 4′,6-diamidino-2-phenylindole channel. Bars = 50 μm.

    Journal: Kidney International Reports

    Article Title: Secondary IgA Nephropathy Shares the Same Immune Features With Primary IgA Nephropathy

    doi: 10.1016/j.ekir.2019.10.012

    Figure Lengend Snippet: Glomerular deposition of galactose-deficient IgA1 in patients with types of secondary IgA nephropathy (IgANs) and controls. Double staining with anti−Gd-IgA1 mAb (red) and anti-IgA1 mAb (green). Merged figures were also added to the 4′,6-diamidino-2-phenylindole channel. Bars = 50 μm.

    Article Snippet: In a French cohort, galactose deficiency and decreased sialylation of IgA1, as well as increased amounts of abnormally glycosylated polymeric IgA1, were detected in the serum of patients with advanced alcoholic cirrhosis.

    Techniques: Double Staining

    Plasma immunoglobulin A1 (IgA1), galactose-deficient IgA1 (Gd-IgA1), and adjusted galactose-deficient IgA1 (AD-Gd-IgA1) levels in nephropathy controls (NCs), healthy controls (HCs), IgA nephropathy cases (IgANs), and secondary IgA nephropathy cases (S-IgANs). (a) Plasma total IgA1; (b) plasma Gd-IgA1; (c) plasma Ad-Gd-IgA1 (IgA1× Gd-IgA1). Triangles, data for IgANs with concurrent cirrhosis.

    Journal: Kidney International Reports

    Article Title: Secondary IgA Nephropathy Shares the Same Immune Features With Primary IgA Nephropathy

    doi: 10.1016/j.ekir.2019.10.012

    Figure Lengend Snippet: Plasma immunoglobulin A1 (IgA1), galactose-deficient IgA1 (Gd-IgA1), and adjusted galactose-deficient IgA1 (AD-Gd-IgA1) levels in nephropathy controls (NCs), healthy controls (HCs), IgA nephropathy cases (IgANs), and secondary IgA nephropathy cases (S-IgANs). (a) Plasma total IgA1; (b) plasma Gd-IgA1; (c) plasma Ad-Gd-IgA1 (IgA1× Gd-IgA1). Triangles, data for IgANs with concurrent cirrhosis.

    Article Snippet: In a French cohort, galactose deficiency and decreased sialylation of IgA1, as well as increased amounts of abnormally glycosylated polymeric IgA1, were detected in the serum of patients with advanced alcoholic cirrhosis.

    Techniques:

    Detection of circulating IgA/IgG immune complex (IgA/IgG-IC) in nephropathy controls (NCs), healthy controls (HCs), IgA nephropathy cases (IgANs), and secondary IgA nephropathy cases (S-IgANs). (a) IgA1-IgG complex; (b) IgA-IgG complex. Triangles, data for IgANs with concurrent cirrhosis.

    Journal: Kidney International Reports

    Article Title: Secondary IgA Nephropathy Shares the Same Immune Features With Primary IgA Nephropathy

    doi: 10.1016/j.ekir.2019.10.012

    Figure Lengend Snippet: Detection of circulating IgA/IgG immune complex (IgA/IgG-IC) in nephropathy controls (NCs), healthy controls (HCs), IgA nephropathy cases (IgANs), and secondary IgA nephropathy cases (S-IgANs). (a) IgA1-IgG complex; (b) IgA-IgG complex. Triangles, data for IgANs with concurrent cirrhosis.

    Article Snippet: In a French cohort, galactose deficiency and decreased sialylation of IgA1, as well as increased amounts of abnormally glycosylated polymeric IgA1, were detected in the serum of patients with advanced alcoholic cirrhosis.

    Techniques:

    Influence of the 3'RR palindrome on Ig synthesis. ( a ) ELISA analysis of IgG 1 , IgG 2a , IgG 2b , IgG 3 and IgA in supernatants of LPS±IL-4-, INFγ- and TGFβ-stimulated splenocytes of ΔleftPAL, Δ IRIS and wt mice. Data are the mean±s.e.m. of eight experiments with one mouse (8–12 weeks old, male and female). * P

    Journal: Nature Communications

    Article Title: Deciphering the importance of the palindromic architecture of the immunoglobulin heavy-chain 3' regulatory region

    doi: 10.1038/ncomms10730

    Figure Lengend Snippet: Influence of the 3'RR palindrome on Ig synthesis. ( a ) ELISA analysis of IgG 1 , IgG 2a , IgG 2b , IgG 3 and IgA in supernatants of LPS±IL-4-, INFγ- and TGFβ-stimulated splenocytes of ΔleftPAL, Δ IRIS and wt mice. Data are the mean±s.e.m. of eight experiments with one mouse (8–12 weeks old, male and female). * P

    Article Snippet: Culture supernatants and plasma (first dilution to 1:50) from ΔleftPAL, ΔIRIS and wt mice were analysed for the presence of the various Ig classes (IgM, IgG1 , IgG2b , IgG2b , IgG3 and IgA) by ELISA, as previously described above , except for the coating made with suitable capture antibodies (2 μg ml−1 for IgM (Southern Biotechnologies, ref: 902001), IgG1 (Southern Biotechnologies, ref: 107001), IgG2a (Southern Biotechnologies, ref: 731956) and IgG2b (Southern Biotechnologies, ref: 731940), 3 μg ml−1 for IgG3 (Southern Biotechnologies, ref: 732371) and 4 μg ml−1 for IgA (Southern Biotechnologies, ref: 104001), blocking performed with 3% BSA in PBS, AP-conjugated goat antisera specific for mouse IgA (Southern Biotechnologies, ref: 104004) and IgG3 (Beckman Coulter, ref: 732374), and for standards made with specific antisera for IgM, IgG1 , IgG2a , IgG2b , IgG3 and IgA (Southern Biotechnologies)).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay

    Serum IgA1 (▴) and IgG (⧫) anti-C-GTF responses after i.n. immunization with liposomal C-GTF. Values are the percent increase in anti-C-GTF activity in serum samples (over three preimmunization samples) collected from human subjects after i.n. immunization with C-GTF (combined data from liposomal C-GTF and free C-GTF groups).

    Journal: Infection and Immunity

    Article Title: A Controlled Clinical Study of the Effect of Nasal Immunization with a Streptococcus mutans Antigen Alone or Incorporated into Liposomes on Induction of Immune Responses

    doi:

    Figure Lengend Snippet: Serum IgA1 (▴) and IgG (⧫) anti-C-GTF responses after i.n. immunization with liposomal C-GTF. Values are the percent increase in anti-C-GTF activity in serum samples (over three preimmunization samples) collected from human subjects after i.n. immunization with C-GTF (combined data from liposomal C-GTF and free C-GTF groups).

    Article Snippet: Immunological reagents used for enzyme-linked immunosorbent assay (ELISA) analysis consisted of biotinylated goat anti-human IgA, IgM, and IgG (Biosource Inc., Burlingame, Calif.); unlabeled rabbit or goat anti-human IgA, IgM, and IgG (Jackson ImmunoResearch Laboratories Inc., West Grove, Pa.); and mouse monoclonal IgG anti-human IgA1 and IgA2 (Accurate Chemical & Scientific Corp., Westbury, N.Y.).

    Techniques: Activity Assay

    Nasal IgA1 anti-C-GTF activity as a percentage of total IgA1 antibody activity before and after i.n. immunization. Values are the ratio of C-GTF-specific/total immunoglobulin for samples collected before and after i.n. immunization with liposomal C-GTF (group A [■]) or free C-GTF (group B [•]). ∗, mean difference between group A and group B values is significantly different ( P

    Journal: Infection and Immunity

    Article Title: A Controlled Clinical Study of the Effect of Nasal Immunization with a Streptococcus mutans Antigen Alone or Incorporated into Liposomes on Induction of Immune Responses

    doi:

    Figure Lengend Snippet: Nasal IgA1 anti-C-GTF activity as a percentage of total IgA1 antibody activity before and after i.n. immunization. Values are the ratio of C-GTF-specific/total immunoglobulin for samples collected before and after i.n. immunization with liposomal C-GTF (group A [■]) or free C-GTF (group B [•]). ∗, mean difference between group A and group B values is significantly different ( P

    Article Snippet: Immunological reagents used for enzyme-linked immunosorbent assay (ELISA) analysis consisted of biotinylated goat anti-human IgA, IgM, and IgG (Biosource Inc., Burlingame, Calif.); unlabeled rabbit or goat anti-human IgA, IgM, and IgG (Jackson ImmunoResearch Laboratories Inc., West Grove, Pa.); and mouse monoclonal IgG anti-human IgA1 and IgA2 (Accurate Chemical & Scientific Corp., Westbury, N.Y.).

    Techniques: Activity Assay

    Salivary IgA anti-C-GTF activity as a percentage of total IgA antibody activity before and after i.n. immunization. Values are the mean ratio of C-GTF-specific/total immunoglobulin (plus standard deviation) in anti-C-GTF activity in parotid saliva samples collected from human subjects before and after i.n. immunization with C-GTF (combined data from liposomal C-GTF and free C-GTF groups).

    Journal: Infection and Immunity

    Article Title: A Controlled Clinical Study of the Effect of Nasal Immunization with a Streptococcus mutans Antigen Alone or Incorporated into Liposomes on Induction of Immune Responses

    doi:

    Figure Lengend Snippet: Salivary IgA anti-C-GTF activity as a percentage of total IgA antibody activity before and after i.n. immunization. Values are the mean ratio of C-GTF-specific/total immunoglobulin (plus standard deviation) in anti-C-GTF activity in parotid saliva samples collected from human subjects before and after i.n. immunization with C-GTF (combined data from liposomal C-GTF and free C-GTF groups).

    Article Snippet: Immunological reagents used for enzyme-linked immunosorbent assay (ELISA) analysis consisted of biotinylated goat anti-human IgA, IgM, and IgG (Biosource Inc., Burlingame, Calif.); unlabeled rabbit or goat anti-human IgA, IgM, and IgG (Jackson ImmunoResearch Laboratories Inc., West Grove, Pa.); and mouse monoclonal IgG anti-human IgA1 and IgA2 (Accurate Chemical & Scientific Corp., Westbury, N.Y.).

    Techniques: Activity Assay, Standard Deviation

    The representative figure showed that SO 2 inhalation did not affect IgG levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : IgA level in the serum; f : IgA level in the nasal septum

    Journal: Journal of Occupational Medicine and Toxicology (London, England)

    Article Title: Sulfur dioxide exposure reduces the quantity of CD19+ cells and causes nasal epithelial injury in rats

    doi: 10.1186/s12995-018-0205-x

    Figure Lengend Snippet: The representative figure showed that SO 2 inhalation did not affect IgG levels in the serum and nasal septum. a : IgG level in the serum; b : IgG level in the nasal septum; c : IgE level in the serum; d : IgE level in the nasal septum; e : IgA level in the serum; f : IgA level in the nasal septum

    Article Snippet: Rat IgG, IgA and IgE ELISA kit was purchased from eBioscience Corp. (USA).

    Techniques:

    Arithmetic means (± standard deviations) of the percentages of IgA1- and IgA2-secreting cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Expression of Homing Receptors on IgA1 and IgA2 Plasmablasts in Blood Reflects Differential Distribution of IgA1 and IgA2 in Various Body Fluids ▿

    doi: 10.1128/CVI.00475-09

    Figure Lengend Snippet: Arithmetic means (± standard deviations) of the percentages of IgA1- and IgA2-secreting cells in peripheral blood expressing various lymphocyte markers. The numbers of unimmunized healthy volunteers from whom the data were pooled are given under the bars. Statistically significant differences between IgA1 and IgA2 ISC in paired Student's t test are indicated by an asterisk (*, 0.01

    Article Snippet: Bound antibodies were detected with biotin-labeled mouse monoclonal IgG anti-human IgA1 or IgA2 (Nordic) followed by ExtrAvidin peroxidase conjugate (Sigma-Aldrich).

    Techniques: Expressing

    Arithmetic means (± standard deviations) of the percentages of antigen-specific IgA1- and IgA2-secreting cells expressing the indicated HR in healthy volunteers after oral vaccination with live attenuated Salmonella serovar Typhi Ty21a (A) or parenteral vaccination with an unconjugated pneumococcal polysaccharide vaccine (B). The numbers of volunteers from whom the data were pooled are given under the bars. There were no statistically significant differences between IgA1 and IgA2 ASC in the paired Student's t test.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Expression of Homing Receptors on IgA1 and IgA2 Plasmablasts in Blood Reflects Differential Distribution of IgA1 and IgA2 in Various Body Fluids ▿

    doi: 10.1128/CVI.00475-09

    Figure Lengend Snippet: Arithmetic means (± standard deviations) of the percentages of antigen-specific IgA1- and IgA2-secreting cells expressing the indicated HR in healthy volunteers after oral vaccination with live attenuated Salmonella serovar Typhi Ty21a (A) or parenteral vaccination with an unconjugated pneumococcal polysaccharide vaccine (B). The numbers of volunteers from whom the data were pooled are given under the bars. There were no statistically significant differences between IgA1 and IgA2 ASC in the paired Student's t test.

    Article Snippet: Bound antibodies were detected with biotin-labeled mouse monoclonal IgG anti-human IgA1 or IgA2 (Nordic) followed by ExtrAvidin peroxidase conjugate (Sigma-Aldrich).

    Techniques: Expressing

    J8 and S2-specific antibody response and spleen cell proliferative response to antigens for individual BALB/c mice (n = 5/group). Mean antibody titer + SEM are shown. ( A ) J8-specific salivary IgA antibody response for BALB/c mice (n = 5/group). Mean antibody titers are represented as a bar + SEM. ( B ) S2-specific salivary IgA titer. ( C ) Average stimulation index (SI) of spleen cell proliferation for BALB/c mice immunized with 30 μg of S2 peptide and 30 μg of DT in complete Freund’s adjuvant. Proliferation of cells induced by S2 peptide and DT (30 μg) and concanavalin A (conA) was determined. The average counts per minute observed for control mice in the absence of antigen were 5303 cpm. ( D ) S2-specific serum IgG titer. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test to compare test groups to the PBS control group (ns, p > 0.05; * p

    Journal: Scientific Reports

    Article Title: Novel platform technology for modular mucosal vaccine that protects against streptococcus

    doi: 10.1038/srep39274

    Figure Lengend Snippet: J8 and S2-specific antibody response and spleen cell proliferative response to antigens for individual BALB/c mice (n = 5/group). Mean antibody titer + SEM are shown. ( A ) J8-specific salivary IgA antibody response for BALB/c mice (n = 5/group). Mean antibody titers are represented as a bar + SEM. ( B ) S2-specific salivary IgA titer. ( C ) Average stimulation index (SI) of spleen cell proliferation for BALB/c mice immunized with 30 μg of S2 peptide and 30 μg of DT in complete Freund’s adjuvant. Proliferation of cells induced by S2 peptide and DT (30 μg) and concanavalin A (conA) was determined. The average counts per minute observed for control mice in the absence of antigen were 5303 cpm. ( D ) S2-specific serum IgG titer. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test to compare test groups to the PBS control group (ns, p > 0.05; * p

    Article Snippet: The plates were washed 5 times and peroxidase-conjugated goat anti-mouse IgG or IgA (Invivogen, San Diego, United States) were added at a dilution of 1:3000 or 1:1000 respectively in 0.5% skim milk PBS-Tween 20 for 1.5 h at 37 °C.

    Techniques: Mouse Assay, MANN-WHITNEY

    J8-specific antibody response and bacterial burden after intranasal challenge with M1 GAS strain in BALB/c mice (n = 10/group). Mean antibody titers are represented as a bar + SEM. Bacterial burden results are represented as the mean CFU + SEM for 10 mice/group on days 1–3 for throat swabs and nasal shedding, and day 3 for NALT. ( A ) Salivary IgA titer. ( B ) Serum IgG titer. ( C ) Nasal shedding. ( D ) Throat swabs. ( E ) Colonization of NALT. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test to compare test groups to the PBS control group (ns, p > 0.05; * p

    Journal: Scientific Reports

    Article Title: Novel platform technology for modular mucosal vaccine that protects against streptococcus

    doi: 10.1038/srep39274

    Figure Lengend Snippet: J8-specific antibody response and bacterial burden after intranasal challenge with M1 GAS strain in BALB/c mice (n = 10/group). Mean antibody titers are represented as a bar + SEM. Bacterial burden results are represented as the mean CFU + SEM for 10 mice/group on days 1–3 for throat swabs and nasal shedding, and day 3 for NALT. ( A ) Salivary IgA titer. ( B ) Serum IgG titer. ( C ) Nasal shedding. ( D ) Throat swabs. ( E ) Colonization of NALT. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test to compare test groups to the PBS control group (ns, p > 0.05; * p

    Article Snippet: The plates were washed 5 times and peroxidase-conjugated goat anti-mouse IgG or IgA (Invivogen, San Diego, United States) were added at a dilution of 1:3000 or 1:1000 respectively in 0.5% skim milk PBS-Tween 20 for 1.5 h at 37 °C.

    Techniques: Mouse Assay, MANN-WHITNEY

    J8 and S2-specific antibody response and bacterial burden after intranasal challenge with 5448AP GAS strain in BALB/c mice (n = 10/group). Mean antibody titer + SEM are shown. ( A ) J8-specific salivary IgA titer. ( B ) J8-specific serum IgG titer. ( C ) S2-specific salivary IgA titer. ( D ) S2- serum IgG titer. ( E ) Nasal shedding. ( F ) Throat swabs. ( G ) Colonization of NALT. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test to compare test groups to the PBS control group (ns, p > 0.05; * p

    Journal: Scientific Reports

    Article Title: Novel platform technology for modular mucosal vaccine that protects against streptococcus

    doi: 10.1038/srep39274

    Figure Lengend Snippet: J8 and S2-specific antibody response and bacterial burden after intranasal challenge with 5448AP GAS strain in BALB/c mice (n = 10/group). Mean antibody titer + SEM are shown. ( A ) J8-specific salivary IgA titer. ( B ) J8-specific serum IgG titer. ( C ) S2-specific salivary IgA titer. ( D ) S2- serum IgG titer. ( E ) Nasal shedding. ( F ) Throat swabs. ( G ) Colonization of NALT. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test to compare test groups to the PBS control group (ns, p > 0.05; * p

    Article Snippet: The plates were washed 5 times and peroxidase-conjugated goat anti-mouse IgG or IgA (Invivogen, San Diego, United States) were added at a dilution of 1:3000 or 1:1000 respectively in 0.5% skim milk PBS-Tween 20 for 1.5 h at 37 °C.

    Techniques: Mouse Assay, MANN-WHITNEY

    Breast milk IgA levels specific to casein (A) and beta-lactoglobulin (BLG) (B) in infants with no cow’s milk allergy (CMA) and in those with either immediate-type or delayed-type CMA. On the box plots the median is represented by a horizontal line within the box representing the 25 th to 75 th percentile, whiskers show the 5 th to 95 th percentile and the square symbol is the geometric mean.

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    Article Title: Role of maternal elimination diets and human milk IgA in development of cow’s milk allergy in the infants

    doi: 10.1111/cea.12228

    Figure Lengend Snippet: Breast milk IgA levels specific to casein (A) and beta-lactoglobulin (BLG) (B) in infants with no cow’s milk allergy (CMA) and in those with either immediate-type or delayed-type CMA. On the box plots the median is represented by a horizontal line within the box representing the 25 th to 75 th percentile, whiskers show the 5 th to 95 th percentile and the square symbol is the geometric mean.

    Article Snippet: After washing, biotinylated mouse anti-human IgA1/IgA2 (BD Pharmingen) at 1:1000, IgG 1:20000, IgG1 1:500, or IgG4 1:10000 was applied to the wells followed by incubation of 1 h at room temperature.

    Techniques:

    Breast milk IgA levels specific to casein (A) and beta-lactoglobulin (BLG) (B) in mothers who were not eliminating cow’s milk (Milk) in their diet and in those with strict milk elimination diet. On the box plots the median is represented by a horizontal line within the box representing the 25 th to 75 th percentile, whiskers show the 5 th to 95 th percentile and the square symbol is the geometric mean.

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    Article Title: Role of maternal elimination diets and human milk IgA in development of cow’s milk allergy in the infants

    doi: 10.1111/cea.12228

    Figure Lengend Snippet: Breast milk IgA levels specific to casein (A) and beta-lactoglobulin (BLG) (B) in mothers who were not eliminating cow’s milk (Milk) in their diet and in those with strict milk elimination diet. On the box plots the median is represented by a horizontal line within the box representing the 25 th to 75 th percentile, whiskers show the 5 th to 95 th percentile and the square symbol is the geometric mean.

    Article Snippet: After washing, biotinylated mouse anti-human IgA1/IgA2 (BD Pharmingen) at 1:1000, IgG 1:20000, IgG1 1:500, or IgG4 1:10000 was applied to the wells followed by incubation of 1 h at room temperature.

    Techniques:

    A. Saturation isotherm and Scatchard analysis of 125 I-UG binding to Fn . Binding isotherms of hUG to Fn were performed by incubating increasing concentrations of 125 I-UG. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of hUG, from the total binding (■). The data shown are the mean of triplicate measurements±SD. B. Scatchard plots of hUG binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 15 nM ± 2.6 nM (n = 3). C. Binding isotherms of IgA to Fn. Binding isotherms of IgA to Fn were performed by incubating increasing concentration of 125 I-IgA. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of IgA, from the total binding (■). The data shown are the mean of triplicate measurements. D. Scatchard plots of IgA binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 65 nM ± 5.2 nM (n = 3).

    Journal: FEBS letters

    Article Title: Uteroglobin interacts with the heparin-binding site of fibronectin and prevents fibronectin-IgA complex formation found in IgA-nephropathy

    doi: 10.1016/j.febslet.2008.01.025

    Figure Lengend Snippet: A. Saturation isotherm and Scatchard analysis of 125 I-UG binding to Fn . Binding isotherms of hUG to Fn were performed by incubating increasing concentrations of 125 I-UG. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of hUG, from the total binding (■). The data shown are the mean of triplicate measurements±SD. B. Scatchard plots of hUG binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 15 nM ± 2.6 nM (n = 3). C. Binding isotherms of IgA to Fn. Binding isotherms of IgA to Fn were performed by incubating increasing concentration of 125 I-IgA. Specific binding (■) was calculated by subtracting nonspecific binding (▲), measured as the residual binding in the presence of IgA, from the total binding (■). The data shown are the mean of triplicate measurements. D. Scatchard plots of IgA binding to Fn. The apparent dissociation constant (K d ) estimated from the slope of the line was 65 nM ± 5.2 nM (n = 3).

    Article Snippet: Immunoglobulin A (IgA)-nephropathy (IgAN) is the most common primary renal glomerular disease in the world that has no effective treatment.

    Techniques: Binding Assay, Concentration Assay

    Normalized matricellular proteins and risk of developing BPD or death. Normalized SPARC levels were significantly higher in babies developing BPD or death prior to 36 weeks postmenstrual age (*p = 0.0185, Mann Whitney U test). There were no other significant differences in normalized matricellular protein concentration between infants who did not develop BPD and infants who developed BPD or died prior to 36 weeks postmenstrual age. Tracheal aspirate matricellular protein and secretory component of IgA (scIgA) concentrations were measured by ELISA or multiplex immune assay. The data show individual matricellular protein levels normalized to scIgA concentration. Medians and interquartile ranges are shown.

    Journal: PLoS ONE

    Article Title: Tracheal Aspirate Levels of the Matricellular Protein SPARC Predict Development of Bronchopulmonary Dysplasia

    doi: 10.1371/journal.pone.0144122

    Figure Lengend Snippet: Normalized matricellular proteins and risk of developing BPD or death. Normalized SPARC levels were significantly higher in babies developing BPD or death prior to 36 weeks postmenstrual age (*p = 0.0185, Mann Whitney U test). There were no other significant differences in normalized matricellular protein concentration between infants who did not develop BPD and infants who developed BPD or died prior to 36 weeks postmenstrual age. Tracheal aspirate matricellular protein and secretory component of IgA (scIgA) concentrations were measured by ELISA or multiplex immune assay. The data show individual matricellular protein levels normalized to scIgA concentration. Medians and interquartile ranges are shown.

    Article Snippet: To control for sample dilution, matricellular protein levels were normalized to the secretory component of immunoglobulin A (scIgA) (ALPCO, Salem, NH) [ ].

    Techniques: MANN-WHITNEY, Protein Concentration, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Concentration Assay

    Mesenchymal stromal cell (MSC) isolation does not influence tracheal aspirate matricellular protein concentration. Tracheal aspirate matricellular protein and secretory component of IgA (scIgA) concentrations were measured by ELISA or multiplex immune assay. The isolation of MSCs from tracheal aspirates had no influence on matricellular protein concentration. The data show individual matricellular protein levels normalized to scIgA concentration. Medians and interquartile ranges are shown. Statistical significance was determined by Mann-Whitney U test.

    Journal: PLoS ONE

    Article Title: Tracheal Aspirate Levels of the Matricellular Protein SPARC Predict Development of Bronchopulmonary Dysplasia

    doi: 10.1371/journal.pone.0144122

    Figure Lengend Snippet: Mesenchymal stromal cell (MSC) isolation does not influence tracheal aspirate matricellular protein concentration. Tracheal aspirate matricellular protein and secretory component of IgA (scIgA) concentrations were measured by ELISA or multiplex immune assay. The isolation of MSCs from tracheal aspirates had no influence on matricellular protein concentration. The data show individual matricellular protein levels normalized to scIgA concentration. Medians and interquartile ranges are shown. Statistical significance was determined by Mann-Whitney U test.

    Article Snippet: To control for sample dilution, matricellular protein levels were normalized to the secretory component of immunoglobulin A (scIgA) (ALPCO, Salem, NH) [ ].

    Techniques: Isolation, Protein Concentration, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Concentration Assay, MANN-WHITNEY

    Total influenza‐specific systemic and mucosal IgG and IgA antibodies. ELISA plates were coated with vaccine (H5N1) or challenge (H1N1) virus. Titers of influenza‐specific IgG and IgA antibodies against both viruses were measured in pre‐challenge sera and vaginal washes obtained from mice vaccinated as in Figure 1 . As a positive control, pooled sera obtained from mice primed intraperitoneally with live PR 8 (H1N1) virus were used. Experiments were performed twice. Bars represent mean antibody titers ± SEM of six mice per group. * * P

    Journal: Influenza and Other Respiratory Viruses

    Article Title: Heterosubtypic cross‐protection induced by whole inactivated influenza virus vaccine in mice: influence of the route of vaccine administration) Heterosubtypic cross‐protection induced by whole inactivated influenza virus vaccine in mice: influence of the route of vaccine administration

    doi: 10.1111/irv.12142

    Figure Lengend Snippet: Total influenza‐specific systemic and mucosal IgG and IgA antibodies. ELISA plates were coated with vaccine (H5N1) or challenge (H1N1) virus. Titers of influenza‐specific IgG and IgA antibodies against both viruses were measured in pre‐challenge sera and vaginal washes obtained from mice vaccinated as in Figure 1 . As a positive control, pooled sera obtained from mice primed intraperitoneally with live PR 8 (H1N1) virus were used. Experiments were performed twice. Bars represent mean antibody titers ± SEM of six mice per group. * * P

    Article Snippet: After washing, plates were incubated for 1 hour with HRP‐conjugated goat anti‐mouse IgG or IgA antibody (Southern Biotech, Birmingham, AL, USA) followed by 30‐min staining with o‐phenylene‐diamine staining solution.

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Positive Control

    Sedimentation Velocity Analysis of IgA1 κ, λ and Mce

    Journal:

    Article Title: Analysis of IgA1 N-glycosylation and its contribution to Fc?RI binding †

    doi: 10.1021/bi801185b

    Figure Lengend Snippet: Sedimentation Velocity Analysis of IgA1 κ, λ and Mce

    Article Snippet: Western blots using anti-IgA1 (Southern Biotech, Birmingham, AL) showed expression of Fcα.

    Techniques: Sedimentation

    Representative LTQ tandem mass spectrum of an IgA1 N -glycopeptide

    Journal:

    Article Title: Analysis of IgA1 N-glycosylation and its contribution to Fc?RI binding †

    doi: 10.1021/bi801185b

    Figure Lengend Snippet: Representative LTQ tandem mass spectrum of an IgA1 N -glycopeptide

    Article Snippet: Western blots using anti-IgA1 (Southern Biotech, Birmingham, AL) showed expression of Fcα.

    Techniques:

    Boxplot IgA level before and after treatment.

    Journal: Advances in Preventive Medicine

    Article Title: Nephrolepis exaltata Herbal Mask Increases Nasal IgA Levels and Pulmonary Function in Textile Factory Workers

    doi: 10.1155/2019/5687135

    Figure Lengend Snippet: Boxplot IgA level before and after treatment.

    Article Snippet: After the above procedure, samples were assessed using ELISA (Enzyme-Linked Immunosorbent Assay) methodology with Human IgA (Immunoglobulin A) ELISA Kit from Elabscience® (catalog number E-EL-H1355).

    Techniques:

    Mean of delta score of IgA level between two groups.

    Journal: Advances in Preventive Medicine

    Article Title: Nephrolepis exaltata Herbal Mask Increases Nasal IgA Levels and Pulmonary Function in Textile Factory Workers

    doi: 10.1155/2019/5687135

    Figure Lengend Snippet: Mean of delta score of IgA level between two groups.

    Article Snippet: After the above procedure, samples were assessed using ELISA (Enzyme-Linked Immunosorbent Assay) methodology with Human IgA (Immunoglobulin A) ELISA Kit from Elabscience® (catalog number E-EL-H1355).

    Techniques:

    The rate of G. vaginalis sialic acid (Neu5Ac) consumption from sialoglycans is substrate-dependent and inhibited by N -glycolylneuraminic acid (Neu5Gc). Washed G. vaginalis JCP8151B was incubated with 0.2 mg/ml of human IgA or 0.3 mg/ml of BSM, followed

    Journal: The Journal of Biological Chemistry

    Article Title: Degradation, Foraging, and Depletion of Mucus Sialoglycans by the Vagina-adapted Actinobacterium Gardnerella vaginalis *

    doi: 10.1074/jbc.M113.453654

    Figure Lengend Snippet: The rate of G. vaginalis sialic acid (Neu5Ac) consumption from sialoglycans is substrate-dependent and inhibited by N -glycolylneuraminic acid (Neu5Gc). Washed G. vaginalis JCP8151B was incubated with 0.2 mg/ml of human IgA or 0.3 mg/ml of BSM, followed

    Article Snippet: The bacterial suspensions were supplemented with human myeloma serum immunoglobulin A-IgA (Kent Laboratories) or bovine submaxillary mucin-BSM (Sigma) at a final concentration of 400 μg/ml.

    Techniques: Incubation

    A , extracellular hydrolysis of human IgA sialic acids by G. vaginalis precedes consumption. Clinical isolates JCP8151B (sialidase-positive) and JCP8481A (sialidase-negative) were incubated with purified human serum IgA in acetate buffer and the concentration

    Journal: The Journal of Biological Chemistry

    Article Title: Degradation, Foraging, and Depletion of Mucus Sialoglycans by the Vagina-adapted Actinobacterium Gardnerella vaginalis *

    doi: 10.1074/jbc.M113.453654

    Figure Lengend Snippet: A , extracellular hydrolysis of human IgA sialic acids by G. vaginalis precedes consumption. Clinical isolates JCP8151B (sialidase-positive) and JCP8481A (sialidase-negative) were incubated with purified human serum IgA in acetate buffer and the concentration

    Article Snippet: The bacterial suspensions were supplemented with human myeloma serum immunoglobulin A-IgA (Kent Laboratories) or bovine submaxillary mucin-BSM (Sigma) at a final concentration of 400 μg/ml.

    Techniques: Incubation, Purification, Concentration Assay