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    IGAN Bio iga nephropathy
    Biochemical features of purified <t>IgA1</t> mAbs. (A) Detection of J chain by Western blot. (B) Analysis of monomeric (mIgA) and polymeric (pIgA) forms by Western blot after SDS-PAGE in nonreducing conditions. (C) Glycosylation of IgA1 mAbs that deposit or
    Iga Nephropathy, supplied by IGAN Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga nephropathy/product/IGAN Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iga nephropathy - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    97
    Thermo Fisher goat anti mouse secondary antibody
    Biochemical features of purified <t>IgA1</t> mAbs. (A) Detection of J chain by Western blot. (B) Analysis of monomeric (mIgA) and polymeric (pIgA) forms by Western blot after SDS-PAGE in nonreducing conditions. (C) Glycosylation of IgA1 mAbs that deposit or
    Goat Anti Mouse Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse secondary antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse secondary antibody - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    99
    Thermo Fisher iga
    Biochemical features of purified <t>IgA1</t> mAbs. (A) Detection of J chain by Western blot. (B) Analysis of monomeric (mIgA) and polymeric (pIgA) forms by Western blot after SDS-PAGE in nonreducing conditions. (C) Glycosylation of IgA1 mAbs that deposit or
    Iga, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iga - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    95
    Millipore iga
    Biochemical features of purified <t>IgA1</t> mAbs. (A) Detection of J chain by Western blot. (B) Analysis of monomeric (mIgA) and polymeric (pIgA) forms by Western blot after SDS-PAGE in nonreducing conditions. (C) Glycosylation of IgA1 mAbs that deposit or
    Iga, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iga - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    N/A
    Blockers are used in ELISA and LF assays to reduce interference from proteins in patient samples that could produce false results and lead to an incorrect diagnosis Choosing the best
      Buy from Supplier


    N/A
    IgA1 antibody Isotype Note IgG1 Host Note Mouse Conjugation Note Unconjugated Application Note ELISA
      Buy from Supplier

    N/A
    Purified Human IgA1 protein
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    Image Search Results


    Biochemical features of purified IgA1 mAbs. (A) Detection of J chain by Western blot. (B) Analysis of monomeric (mIgA) and polymeric (pIgA) forms by Western blot after SDS-PAGE in nonreducing conditions. (C) Glycosylation of IgA1 mAbs that deposit or

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Biochemical features of purified IgA1 mAbs. (A) Detection of J chain by Western blot. (B) Analysis of monomeric (mIgA) and polymeric (pIgA) forms by Western blot after SDS-PAGE in nonreducing conditions. (C) Glycosylation of IgA1 mAbs that deposit or

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Purification, Western Blot, SDS Page

    Calculated pI of combined VH and V κ CDR regions from α 1KI IgA1 hybridomas. Amino acid sequences of VH and V κ CDR from hybridomas 1–10 were combined to calculate pI; α 1KI hybridomas yielding (circles) or not yielding

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Calculated pI of combined VH and V κ CDR regions from α 1KI IgA1 hybridomas. Amino acid sequences of VH and V κ CDR from hybridomas 1–10 were combined to calculate pI; α 1KI hybridomas yielding (circles) or not yielding

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques:

    IgA production and deposition vary with housing conditions. Influence of environmental stimuli on (A) mesangial IgA deposition and (B) plasma IgA levels in 6-week-old and 3-month-old α 1KI mice housed in GF, SOPF, or CIS facilities. Homogeneous

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: IgA production and deposition vary with housing conditions. Influence of environmental stimuli on (A) mesangial IgA deposition and (B) plasma IgA levels in 6-week-old and 3-month-old α 1KI mice housed in GF, SOPF, or CIS facilities. Homogeneous

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Mouse Assay

    In vivo deposited IgA in RAG-2/ γ c–deficient mice after intraperitoneal grafting of α 1KI hybridomas 1, 5, 6, 9, and 10. Each hybridoma was injected in two mice, which were euthanized after tumor development. Ctrl− stands

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: In vivo deposited IgA in RAG-2/ γ c–deficient mice after intraperitoneal grafting of α 1KI hybridomas 1, 5, 6, 9, and 10. Each hybridoma was injected in two mice, which were euthanized after tumor development. Ctrl− stands

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: In Vivo, Mouse Assay, Injection

    IgA glycosylation varies with housing conditions. Analysis of (A) N -acetylneuraminic acid and (B) exposed GalNac in plasma IgA from 6-week-old and 3-month-old α 1KI, α 1KI J−/−, and wild-type (WT) mice housed in GF, SOPF,

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: IgA glycosylation varies with housing conditions. Analysis of (A) N -acetylneuraminic acid and (B) exposed GalNac in plasma IgA from 6-week-old and 3-month-old α 1KI, α 1KI J−/−, and wild-type (WT) mice housed in GF, SOPF,

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Mouse Assay

    Role of mAb V regions in kidney deposition. IgA1 mAb6 or its IgG1 switched variant was injected in immunodeficient mice 2 hours before euthanasia. IgA1 and IgG1 were revealed on kidney sections with FITC-labeled (green) antisera against either (upper

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Role of mAb V regions in kidney deposition. IgA1 mAb6 or its IgG1 switched variant was injected in immunodeficient mice 2 hours before euthanasia. IgA1 and IgG1 were revealed on kidney sections with FITC-labeled (green) antisera against either (upper

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Variant Assay, Injection, Mouse Assay, Labeling

    Influence of environmental stimuli on the monomeric-to-polymeric ratio of circulating IgA in groups of young (6–8 weeks old) and adult (3–4 months old) mice housed in GF, SOPF, or CIS facilities. (A) Plasma proteins were separated under

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Influence of environmental stimuli on the monomeric-to-polymeric ratio of circulating IgA in groups of young (6–8 weeks old) and adult (3–4 months old) mice housed in GF, SOPF, or CIS facilities. (A) Plasma proteins were separated under

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Mouse Assay

    Kinetics of plasma IgA levels and IgA deposition in glomeruli of 1-week-old to 3-month-old α 1KI and α 1KI J−/− mice. (A) IgA was measured by ELISA in mice housed in CIS facilities (means±SEM of n =4–8 mice;

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Kinetics of plasma IgA levels and IgA deposition in glomeruli of 1-week-old to 3-month-old α 1KI and α 1KI J−/− mice. (A) IgA was measured by ELISA in mice housed in CIS facilities (means±SEM of n =4–8 mice;

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    IgA1 mesangial deposits in adult α 1KI mice housed in CIS facilities. (A) Kidney section stained with FITC–conjugated anti–human IgA (green). (B) Electron microscopy of kidney section: white arrow points to IgA deposits. (C) Electron

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: IgA1 mesangial deposits in adult α 1KI mice housed in CIS facilities. (A) Kidney section stained with FITC–conjugated anti–human IgA (green). (B) Electron microscopy of kidney section: white arrow points to IgA deposits. (C) Electron

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Mouse Assay, Staining, Electron Microscopy

    Western blot analysis of trypsin digestion products. Nine IgA1 mAbs were submitted to 10% SDS-PAGE after various digestion times (0–10 hours) by trypsin. Western blot was performed to reveal IgA determinants using HRP–linked goat anti–human

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Western blot analysis of trypsin digestion products. Nine IgA1 mAbs were submitted to 10% SDS-PAGE after various digestion times (0–10 hours) by trypsin. Western blot was performed to reveal IgA determinants using HRP–linked goat anti–human

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Western Blot, SDS Page

    Circular dichroism analysis of IgA1 mAbs. (A) Far-ultraviolet spectra of six mAbs acquired at 20°C with a path length of 0.2 cm on a spectropolarimeter. IgA1 mAb1 and IgA1 mAb6 yield IgA deposition, and the others do not. (B) Thermal unfolding

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Circular dichroism analysis of IgA1 mAbs. (A) Far-ultraviolet spectra of six mAbs acquired at 20°C with a path length of 0.2 cm on a spectropolarimeter. IgA1 mAb1 and IgA1 mAb6 yield IgA deposition, and the others do not. (B) Thermal unfolding

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques:

    Analysis of IgA deposition in the kidney of immunodeficient mice 2 hours after injection of human IgA1 mAb. Clones 1–10 were injected in two mice each. Ctrl− is a negative control after injection of saline. (A) IgA deposits in kidney sections

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: IgA Structure Variations Associate with Immune Stimulations and IgA Mesangial Deposition

    doi: 10.1681/ASN.2015080911

    Figure Lengend Snippet: Analysis of IgA deposition in the kidney of immunodeficient mice 2 hours after injection of human IgA1 mAb. Clones 1–10 were injected in two mice each. Ctrl− is a negative control after injection of saline. (A) IgA deposits in kidney sections

    Article Snippet: Suzuki H, Fan R, Zhang Z, Brown R, Hall S, Julian BA, Chatham WW, Suzuki Y, Wyatt RJ, Moldoveanu Z, Lee JY, Robinson J, Tomana M, Tomino Y, Mestecky J, Novak J.: Aberrantly glycosylated IgA1 in IgA nephropathy patients is recognized by IgG antibodies with restricted heterogeneity.

    Techniques: Mouse Assay, Injection, Clone Assay, Negative Control