Journal: Frontiers in Immunology
Article Title: Endothelial Cell Amplification of Regulatory T Cells Is Differentially Modified by Immunosuppressors and Intravenous Immunoglobulin
Figure Lengend Snippet: Effect of endothelial cell (EC) incubation with mycophenolic acid (MPA), cyclosporine A (CsA), and intravenous immunoglobulin (IVIg) on the proinflammatory activity of ECs. IL-6 secretion was quantified in the supernatants of cocultures where ECs was treated with MPA [ (A) , n > 19 donors], CsA [ (B) , n = 24 donors] or IVIg [ (C) , n > 6 donors]. IL-6 production by ECs incubated with vehicle alone is represented as 0. (D–F) show the expansion of the Th17 subset after a 7 day coculture of PBMC with EC pretreated with interferon γ (IFN-γ) and the indicated dose of MPA [ (D) , n = 7 donors], CsA [ (E) , n = 9 donors], or IVIg [ (F) , n > 18 donors]. Expansion of the Th1 subset under the same conditions is shown in [ (G) , n > 7 donors], [ (H) , n = 9 donors], and [ (I) , n > 18 donors]. Results are expressed as the relative percentage of the control values (ECs treated with vehicle alone, represented by dotted lines). Thick, horizontal lines represent median values in all the cocultures (* p
Article Snippet: ECs Pretreatment with Immunosuppressors Endothelial cells were cultured with interferon γ (IFN-γ) (200 IU/ml for 3 days; R & D Systems, Minneapolis, MN, USA) in tissue culture flasks and incubated, where indicated, with immunosuppressors: MPA (Sigma-Aldrich), CsA (Sandimmun® , Novartis), or with IVIg (Privigen® , CSL Behring) at the indicated concentrations.
Techniques: Incubation, Activity Assay