Journal: Aging Cell

Article Title: Accelerated microglial pathology is associated with Aβ plaques in mouse models of Alzheimer’s disease

doi: 10.1111/acel.12210

Figure Lengend Snippet: Microglial expression of CD39 is altered in mouse models of Alzheimer’s disease. IbaI and CD39 IHC analyses were performed on brain sections from young, old, and amyloid precursor proteins (APP) Tg mice as described in Experimental procedures. (A, B) Representative z-stack images of WT (aged 3 months) and APP sw,Ind Tg mice (aged 9 months), showing IbaI+ cells (Aa, Ba; green), CD39 + cells (Ab, Bb; red), and the merge images (Ac, Bc) in the cortex. Arrows in Bc point to cells classified as CD39 high (C1), CD39 + (C2), and CD39 low (C3). (C1–C3) Enlargement of cells depicted in Bc. Cells shown in C1 were traced with Simple Neurite Tracer plug-in followed by process ‘filling’ as detailed in Experimental procedures. (C) Representative z-stack images of APP sw,Ind Tg mice showing cells stained for CD68 (Ca), CD39 (Cb), and merge image (Cc). (D) Representative z-stack images of APP sw,Ind Tg mice stained for CD11b (Da), CD39 (Db), and merge image (Dc). (E) Graph showing CD39 mean fluorescent intensity (MFIs) of IbaI + cells in images taken from 3- and 21-month-old WT mice and APP Sw,Ind (aged 9 months), and APP/PS1 (aged 15 months) Tg mice. Average MFIs were calculated for each experimental group and analyzed by a one-way Tukey’s ANOVA. ** P

Article Snippet: The sections were then incubated in primary antibody diluting buffer (Golden Bridge International, Mukilteo, WA, USA) and then transferred for overnight incubation with IbaI antibody (WAKO, Osaka, Japan), CD39 (R & D, Minneapolis, MN, USA), and CD11b (Serotec, Raleigh, NC, USA) at 4 °C.

Techniques: Expressing, Immunohistochemistry, Mouse Assay, Staining

Journal: PLoS ONE

Article Title: An Adeno-Associated Virus-Based Intracellular Sensor of Pathological Nuclear Factor-?B Activation for Disease-Inducible Gene Transfer

doi: 10.1371/journal.pone.0053156

Figure Lengend Snippet: Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with GFP (green fluorescence) and NeuN, GFAP IbaI or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).

Article Snippet: GFP:IbaI co-labeling For GFP:IbaI double immunofluorescence, the above described GFP labeling was combined with goat anti-IbaI (Abcam, cat number: ab 5076) followed by a donkey anti-goat A.568 antibody (Molecular probes, cat number A-11057) diluted 1∶500.

Techniques: Injection, Labeling, Fluorescence, Staining, Confocal Microscopy