Journal: Aging Cell
Article Title: Accelerated microglial pathology is associated with Aβ plaques in mouse models of Alzheimer’s disease
Figure Lengend Snippet: Microglial expression of CD39 is altered in mouse models of Alzheimer’s disease. IbaI and CD39 IHC analyses were performed on brain sections from young, old, and amyloid precursor proteins (APP) Tg mice as described in Experimental procedures. (A, B) Representative z-stack images of WT (aged 3 months) and APP sw,Ind Tg mice (aged 9 months), showing IbaI+ cells (Aa, Ba; green), CD39 + cells (Ab, Bb; red), and the merge images (Ac, Bc) in the cortex. Arrows in Bc point to cells classified as CD39 high (C1), CD39 + (C2), and CD39 low (C3). (C1–C3) Enlargement of cells depicted in Bc. Cells shown in C1 were traced with Simple Neurite Tracer plug-in followed by process ‘filling’ as detailed in Experimental procedures. (C) Representative z-stack images of APP sw,Ind Tg mice showing cells stained for CD68 (Ca), CD39 (Cb), and merge image (Cc). (D) Representative z-stack images of APP sw,Ind Tg mice stained for CD11b (Da), CD39 (Db), and merge image (Dc). (E) Graph showing CD39 mean fluorescent intensity (MFIs) of IbaI + cells in images taken from 3- and 21-month-old WT mice and APP Sw,Ind (aged 9 months), and APP/PS1 (aged 15 months) Tg mice. Average MFIs were calculated for each experimental group and analyzed by a one-way Tukey’s ANOVA. ** P
Article Snippet: The sections were then incubated in primary antibody diluting buffer (Golden Bridge International, Mukilteo, WA, USA) and then transferred for overnight incubation with IbaI antibody (WAKO, Osaka, Japan), CD39 (R & D, Minneapolis, MN, USA), and CD11b (Serotec, Raleigh, NC, USA) at 4 °C.
Techniques: Expressing, Immunohistochemistry, Mouse Assay, Staining