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  • 88
    Thermo Fisher ibai
    Ibai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ibai/product/Thermo Fisher
    Average 88 stars, based on 13 article reviews
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    ibai - by Bioz Stars, 2020-08
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    88
    FUJIFILM ibai antibody
    Microglial expression of <t>CD39</t> is altered in mouse models of Alzheimer’s disease. <t>IbaI</t> and CD39 IHC analyses were performed on brain sections from young, old, and amyloid precursor proteins (APP) Tg mice as described in Experimental procedures. (A, B) Representative z-stack images of WT (aged 3 months) and APP sw,Ind Tg mice (aged 9 months), showing IbaI+ cells (Aa, Ba; green), CD39 + cells (Ab, Bb; red), and the merge images (Ac, Bc) in the cortex. Arrows in Bc point to cells classified as CD39 high (C1), CD39 + (C2), and CD39 low (C3). (C1–C3) Enlargement of cells depicted in Bc. Cells shown in C1 were traced with Simple Neurite Tracer plug-in followed by process ‘filling’ as detailed in Experimental procedures. (C) Representative z-stack images of APP sw,Ind Tg mice showing cells stained for CD68 (Ca), CD39 (Cb), and merge image (Cc). (D) Representative z-stack images of APP sw,Ind Tg mice stained for CD11b (Da), CD39 (Db), and merge image (Dc). (E) Graph showing CD39 mean fluorescent intensity (MFIs) of IbaI + cells in images taken from 3- and 21-month-old WT mice and APP Sw,Ind (aged 9 months), and APP/PS1 (aged 15 months) Tg mice. Average MFIs were calculated for each experimental group and analyzed by a one-way Tukey’s ANOVA. ** P
    Ibai Antibody, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ibai antibody/product/FUJIFILM
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    ibai antibody - by Bioz Stars, 2020-08
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    86
    Abcam anti ibai antibody
    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with <t>GFP</t> (green fluorescence) and NeuN, GFAP <t>IbaI</t> or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).
    Anti Ibai Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ibai antibody/product/Abcam
    Average 86 stars, based on 3 article reviews
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    anti ibai antibody - by Bioz Stars, 2020-08
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    93
    Abcam mouse anti ibai
    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with <t>GFP</t> (green fluorescence) and NeuN, GFAP <t>IbaI</t> or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).
    Mouse Anti Ibai, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ibai/product/Abcam
    Average 93 stars, based on 27 article reviews
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    mouse anti ibai - by Bioz Stars, 2020-08
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    93
    FUJIFILM rabbit anti ibai
    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with <t>GFP</t> (green fluorescence) and NeuN, GFAP <t>IbaI</t> or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).
    Rabbit Anti Ibai, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ibai/product/FUJIFILM
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    88
    Agilent technologies rabbit anti ibai
    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with <t>GFP</t> (green fluorescence) and NeuN, GFAP <t>IbaI</t> or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).
    Rabbit Anti Ibai, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ibai/product/Agilent technologies
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    Biocare Medical rabbit anti ibai
    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with <t>GFP</t> (green fluorescence) and NeuN, GFAP <t>IbaI</t> or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).
    Rabbit Anti Ibai, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    FUJIFILM microglia specific anti ibai antibody
    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with <t>GFP</t> (green fluorescence) and NeuN, GFAP <t>IbaI</t> or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).
    Microglia Specific Anti Ibai Antibody, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    FUJIFILM rabbit polyclonal anti ibai
    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with <t>GFP</t> (green fluorescence) and NeuN, GFAP <t>IbaI</t> or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).
    Rabbit Polyclonal Anti Ibai, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Microglial expression of CD39 is altered in mouse models of Alzheimer’s disease. IbaI and CD39 IHC analyses were performed on brain sections from young, old, and amyloid precursor proteins (APP) Tg mice as described in Experimental procedures. (A, B) Representative z-stack images of WT (aged 3 months) and APP sw,Ind Tg mice (aged 9 months), showing IbaI+ cells (Aa, Ba; green), CD39 + cells (Ab, Bb; red), and the merge images (Ac, Bc) in the cortex. Arrows in Bc point to cells classified as CD39 high (C1), CD39 + (C2), and CD39 low (C3). (C1–C3) Enlargement of cells depicted in Bc. Cells shown in C1 were traced with Simple Neurite Tracer plug-in followed by process ‘filling’ as detailed in Experimental procedures. (C) Representative z-stack images of APP sw,Ind Tg mice showing cells stained for CD68 (Ca), CD39 (Cb), and merge image (Cc). (D) Representative z-stack images of APP sw,Ind Tg mice stained for CD11b (Da), CD39 (Db), and merge image (Dc). (E) Graph showing CD39 mean fluorescent intensity (MFIs) of IbaI + cells in images taken from 3- and 21-month-old WT mice and APP Sw,Ind (aged 9 months), and APP/PS1 (aged 15 months) Tg mice. Average MFIs were calculated for each experimental group and analyzed by a one-way Tukey’s ANOVA. ** P

    Journal: Aging Cell

    Article Title: Accelerated microglial pathology is associated with Aβ plaques in mouse models of Alzheimer’s disease

    doi: 10.1111/acel.12210

    Figure Lengend Snippet: Microglial expression of CD39 is altered in mouse models of Alzheimer’s disease. IbaI and CD39 IHC analyses were performed on brain sections from young, old, and amyloid precursor proteins (APP) Tg mice as described in Experimental procedures. (A, B) Representative z-stack images of WT (aged 3 months) and APP sw,Ind Tg mice (aged 9 months), showing IbaI+ cells (Aa, Ba; green), CD39 + cells (Ab, Bb; red), and the merge images (Ac, Bc) in the cortex. Arrows in Bc point to cells classified as CD39 high (C1), CD39 + (C2), and CD39 low (C3). (C1–C3) Enlargement of cells depicted in Bc. Cells shown in C1 were traced with Simple Neurite Tracer plug-in followed by process ‘filling’ as detailed in Experimental procedures. (C) Representative z-stack images of APP sw,Ind Tg mice showing cells stained for CD68 (Ca), CD39 (Cb), and merge image (Cc). (D) Representative z-stack images of APP sw,Ind Tg mice stained for CD11b (Da), CD39 (Db), and merge image (Dc). (E) Graph showing CD39 mean fluorescent intensity (MFIs) of IbaI + cells in images taken from 3- and 21-month-old WT mice and APP Sw,Ind (aged 9 months), and APP/PS1 (aged 15 months) Tg mice. Average MFIs were calculated for each experimental group and analyzed by a one-way Tukey’s ANOVA. ** P

    Article Snippet: The sections were then incubated in primary antibody diluting buffer (Golden Bridge International, Mukilteo, WA, USA) and then transferred for overnight incubation with IbaI antibody (WAKO, Osaka, Japan), CD39 (R & D, Minneapolis, MN, USA), and CD11b (Serotec, Raleigh, NC, USA) at 4 °C.

    Techniques: Expressing, Immunohistochemistry, Mouse Assay, Staining

    Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with GFP (green fluorescence) and NeuN, GFAP IbaI or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).

    Journal: PLoS ONE

    Article Title: An Adeno-Associated Virus-Based Intracellular Sensor of Pathological Nuclear Factor-?B Activation for Disease-Inducible Gene Transfer

    doi: 10.1371/journal.pone.0053156

    Figure Lengend Snippet: Cellular specificity of rAAV2/1-NF8d1-EGFP-mediated gene transfer in the hippocampus of kainic acid-treated rats. Kainic acid (5 mg/kg) was injected intraperitoneally (ip) one month after stereotaxic injection of rAAV2/1-NF8d1-EGFP into the hippocampus. Fifty µm coronal sections were co-labeled with GFP (green fluorescence) and NeuN, GFAP IbaI or Olig2 . Confocal pictures showing GFP and cell-specific markers co-labeled cells (yellow). White arrows indicate co-labeled cells. Bars represent 50 µm. The mean percentage of GFP-positive cells co-labeling with the GFAP, NeuN and Olig2 markers are shown. No GFP-positive cell stained positive for IbaI. Analysis was performed using confocal microscopy and counting the number co-labeled cells on 5 sections per animal (n = 5 rats for GFP/NeuN and GFP/GFAP, n = 6 for GFP/IbaI and n = 7 for GFP/Olig2 co-labeling).

    Article Snippet: GFP:IbaI co-labeling For GFP:IbaI double immunofluorescence, the above described GFP labeling was combined with goat anti-IbaI (Abcam, cat number: ab 5076) followed by a donkey anti-goat A.568 antibody (Molecular probes, cat number A-11057) diluted 1∶500.

    Techniques: Injection, Labeling, Fluorescence, Staining, Confocal Microscopy