Journal: Scientific Reports
Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production
Figure Lengend Snippet: Knocking down iNOS diminishes the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or iNOS shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16 . ( d ) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p
Article Snippet: Specific knockdown of NFκB and iNOS in hCPCs To specifically knock down NFκB or iNOS in hCPCs, we established the stable cell lines expressing shRNA against NFκB or iNOS in hCPCs using shRNA lentiviral particles according to the manufacturer’s instructions (Santa Cruz Biotechnology).
Techniques: FACS, Staining, Stable Transfection, Expressing, shRNA, Over Expression, Western Blot