Journal: Heliyon

Article Title: Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

doi: 10.1016/j.heliyon.2022.e09936

Figure Lengend Snippet: Proliferation and cytotoxic effects from OGP treatment on hPDLSCs. (A) Schematic experiment and (B) proliferation assay for mitogenic activity using alamarBlue™ assay were presented. (C) Live cells stained with calcein-AM and dead cells stained with propidium iodide were illustrated in green and red, respectively. Bars on proliferation assay result indicated the significant difference ( p <0.05).

Article Snippet: For OGP treatment, OGP was purchased from MedChemExpress (Cat. No. HY-P1563, USA). hPDLSCs were cultured with OGP supplemented in culture medium at concentrations of 0.01, 0.1, 1, 10, and 100 nM according to previous publication ( ).

Techniques: Proliferation Assay, Activity Assay, Alamar Blue Assay, Staining

Journal: Heliyon

Article Title: Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

doi: 10.1016/j.heliyon.2022.e09936

Figure Lengend Snippet: Osteogenic differentiation effects of OGP on hPDLSCs. (A) Schematic experiment of OGP treatment on hPDLSCs toward osteogenic lineage. (B) The ALP activity, (C) osteogenic-related marker expression, (D) mineral deposition, and (E) Alizarin Red S staining quantification were presented. Bars on ALP activity results and Alizarin Red S staining quantification and asterisks on gene expression results indicated the statistically significant difference between OM and other groups ( p <0.05).

Article Snippet: For OGP treatment, OGP was purchased from MedChemExpress (Cat. No. HY-P1563, USA). hPDLSCs were cultured with OGP supplemented in culture medium at concentrations of 0.01, 0.1, 1, 10, and 100 nM according to previous publication ( ).

Techniques: Activity Assay, Marker, Expressing, Staining, Gene Expression

Journal: Heliyon

Article Title: Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

doi: 10.1016/j.heliyon.2022.e09936

Figure Lengend Snippet: Osteogenic-regulated signaling pathway screening of OGP treatment on hPDLSCs toward osteogenic lineage. (A) Schematic experiment of OGP treatment on hPDLSCs to evaluate osteogenic-related pathways was indicated. (B) Bone morphogenetic protein (BMP), (C) Hedgehog, (D) Notch, (E) transforming growth factor-beta (TGF-β), and (F) Wnt signalings related gene expression were presented. Asterisks beside the dot of experimental group on gene expression results indicated the significant difference between OM and other groups ( p <0.05).

Article Snippet: For OGP treatment, OGP was purchased from MedChemExpress (Cat. No. HY-P1563, USA). hPDLSCs were cultured with OGP supplemented in culture medium at concentrations of 0.01, 0.1, 1, 10, and 100 nM according to previous publication ( ).

Techniques: Gene Expression

Journal: Heliyon

Article Title: Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

doi: 10.1016/j.heliyon.2022.e09936

Figure Lengend Snippet: Effect of SB-431542 on OGP-induced osteogenic differentiation in hPDLSCs. (A) Schematic experiment was illustrated. (B) The ALP activity, (C) osteogenic-related marker expression, (D) mineral deposition, and (E) Alizarin Red S staining quantification were presented. Bars and asterisks indicated the significant difference between OM and other groups ( p <0.05).

Article Snippet: For OGP treatment, OGP was purchased from MedChemExpress (Cat. No. HY-P1563, USA). hPDLSCs were cultured with OGP supplemented in culture medium at concentrations of 0.01, 0.1, 1, 10, and 100 nM according to previous publication ( ).

Techniques: Activity Assay, Marker, Expressing, Staining