human tgf-β Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars

  • 99
    R&D Systems recombinant human tgf β
    <t>TGF-β</t> effects the expression of DEC1, DEC2, and EMT-related factors. (A) PC-3 cells were untreated, mock-treated (buffer alone), or treated with 2, 5, 10 ng/ml of TGF-β. After 24 h, total RNA was prepared and subjected to RT-PCR analyses of DEC1, DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. PC-3 cells were treated with TGF-β for 24 h. (B) The cells were lysed and the lysates were subjected to western blot analyses of DEC1, DEC2, pSmad2, E-cadherin, N-cadherin, vimentin, and actin.
    Recombinant Human Tgf β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human tgf β/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human tgf β - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    99
    PeproTech recombinant human tgf β
    <t>TGF-β</t> effects the expression of DEC1, DEC2, and EMT-related factors. (A) PC-3 cells were untreated, mock-treated (buffer alone), or treated with 2, 5, 10 ng/ml of TGF-β. After 24 h, total RNA was prepared and subjected to RT-PCR analyses of DEC1, DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. PC-3 cells were treated with TGF-β for 24 h. (B) The cells were lysed and the lysates were subjected to western blot analyses of DEC1, DEC2, pSmad2, E-cadherin, N-cadherin, vimentin, and actin.
    Recombinant Human Tgf β, supplied by PeproTech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human tgf β/product/PeproTech
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human tgf β - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    99
    R&D Systems human tgf β
    <t>TGF-β</t> effects the expression of DEC1, DEC2, and EMT-related factors. (A) PC-3 cells were untreated, mock-treated (buffer alone), or treated with 2, 5, 10 ng/ml of TGF-β. After 24 h, total RNA was prepared and subjected to RT-PCR analyses of DEC1, DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. PC-3 cells were treated with TGF-β for 24 h. (B) The cells were lysed and the lysates were subjected to western blot analyses of DEC1, DEC2, pSmad2, E-cadherin, N-cadherin, vimentin, and actin.
    Human Tgf β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgf β/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tgf β - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    N/A
    Clone REA903 recognizes the human transforming growth receptor beta 2 TGF β RII antigen a single pass transmembrane protein belonging to the superfamily of serine threonine kinase receptors Members of
      Buy from Supplier

    N/A
    Clone REA903 recognizes the human transforming growth receptor beta 2 TGF β RII antigen a single pass transmembrane protein belonging to the superfamily of serine threonine kinase receptors Members of
      Buy from Supplier

    N/A
    Clone REA903 recognizes the human transforming growth receptor beta 2 TGF β RII antigen a single pass transmembrane protein belonging to the superfamily of serine threonine kinase receptors Members of
      Buy from Supplier

    Image Search Results


    TGF-β effects the expression of DEC1, DEC2, and EMT-related factors. (A) PC-3 cells were untreated, mock-treated (buffer alone), or treated with 2, 5, 10 ng/ml of TGF-β. After 24 h, total RNA was prepared and subjected to RT-PCR analyses of DEC1, DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. PC-3 cells were treated with TGF-β for 24 h. (B) The cells were lysed and the lysates were subjected to western blot analyses of DEC1, DEC2, pSmad2, E-cadherin, N-cadherin, vimentin, and actin.

    Journal: Molecular Medicine Reports

    Article Title: Correlation between DEC1/DEC2 and epithelial-mesenchymal transition in human prostate cancer PC-3 cells

    doi: 10.3892/mmr.2018.9367

    Figure Lengend Snippet: TGF-β effects the expression of DEC1, DEC2, and EMT-related factors. (A) PC-3 cells were untreated, mock-treated (buffer alone), or treated with 2, 5, 10 ng/ml of TGF-β. After 24 h, total RNA was prepared and subjected to RT-PCR analyses of DEC1, DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. PC-3 cells were treated with TGF-β for 24 h. (B) The cells were lysed and the lysates were subjected to western blot analyses of DEC1, DEC2, pSmad2, E-cadherin, N-cadherin, vimentin, and actin.

    Article Snippet: Cells were incubated with various concentrations of recombinant human TGF-β (R & D Systems, Inc., Minneapolis, MN, USA) for the indicated period of time.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Effects of DEC1/DEC2 on cell morphology and the abundance of cytoplasmic EMT-related factors in PC-3 cells. PC-3 cells were transfected with DEC1 siRNA or DEC2 siRNA, followed by culture with or without TGF-β (10 ng/ml) for 24 h. Cell morphological changes were recorded by bright-phase microscopy (magnification, ×400). The cells were then fixed, incubated with antibodies against E-cadherin, N-cadherin, or vimentin and visualized using an Alexa Fluor 488-conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). One representative image (magnification, ×400) of three independent experiments with similar results is shown. The black arrows indicate fibroblast-like cells. DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2; EMT, epithelial-mesenchymal transition.

    Journal: Molecular Medicine Reports

    Article Title: Correlation between DEC1/DEC2 and epithelial-mesenchymal transition in human prostate cancer PC-3 cells

    doi: 10.3892/mmr.2018.9367

    Figure Lengend Snippet: Effects of DEC1/DEC2 on cell morphology and the abundance of cytoplasmic EMT-related factors in PC-3 cells. PC-3 cells were transfected with DEC1 siRNA or DEC2 siRNA, followed by culture with or without TGF-β (10 ng/ml) for 24 h. Cell morphological changes were recorded by bright-phase microscopy (magnification, ×400). The cells were then fixed, incubated with antibodies against E-cadherin, N-cadherin, or vimentin and visualized using an Alexa Fluor 488-conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). One representative image (magnification, ×400) of three independent experiments with similar results is shown. The black arrows indicate fibroblast-like cells. DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2; EMT, epithelial-mesenchymal transition.

    Article Snippet: Cells were incubated with various concentrations of recombinant human TGF-β (R & D Systems, Inc., Minneapolis, MN, USA) for the indicated period of time.

    Techniques: Transfection, Microscopy, Incubation, Staining

    Roles of DEC1/DEC2 in the cell migration of PC-3 cells. PC-3 cells grown in a monolayer were scratched using a pipette tip (P-200 pipette). The cells were then transfected with siRNA against DEC1 or DEC2 and cultured for 24 h. Photographs were taken (magnification, ×40) at 0, 24, and 48 h after adding TGF-β (10 ng/ml). DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2.

    Journal: Molecular Medicine Reports

    Article Title: Correlation between DEC1/DEC2 and epithelial-mesenchymal transition in human prostate cancer PC-3 cells

    doi: 10.3892/mmr.2018.9367

    Figure Lengend Snippet: Roles of DEC1/DEC2 in the cell migration of PC-3 cells. PC-3 cells grown in a monolayer were scratched using a pipette tip (P-200 pipette). The cells were then transfected with siRNA against DEC1 or DEC2 and cultured for 24 h. Photographs were taken (magnification, ×40) at 0, 24, and 48 h after adding TGF-β (10 ng/ml). DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2.

    Article Snippet: Cells were incubated with various concentrations of recombinant human TGF-β (R & D Systems, Inc., Minneapolis, MN, USA) for the indicated period of time.

    Techniques: Migration, Transferring, Transfection, Cell Culture

    (A-H) Opposite roles of DEC1 and DEC2 in regulating EMT induced by TGF-β. PC-3 cells were transfected with the siRNAs (24 h transfection) or the expression plasmids (18 h transfection) of DEC1 and DEC2; subsequently, the cells were incubated with or without TGF-β (10 ng/ml) for an additional 24 h. (A, C, E and G) Total RNA was prepared and subjected to RT-PCR analyses of DEC1 or DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. (B, D, F, and H) Protein was prepared from the cells and subjected to western blot analyses of DEC1 or DEC2, pSmad2, E-cadherin, N-cadherin, vimentin and actin. One representative result of at least three independent experiments with similar results is shown. DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2; EMT, epithelial-mesenchymal transition; RT-PCR, reverse transcription-polymerase chain reaction.

    Journal: Molecular Medicine Reports

    Article Title: Correlation between DEC1/DEC2 and epithelial-mesenchymal transition in human prostate cancer PC-3 cells

    doi: 10.3892/mmr.2018.9367

    Figure Lengend Snippet: (A-H) Opposite roles of DEC1 and DEC2 in regulating EMT induced by TGF-β. PC-3 cells were transfected with the siRNAs (24 h transfection) or the expression plasmids (18 h transfection) of DEC1 and DEC2; subsequently, the cells were incubated with or without TGF-β (10 ng/ml) for an additional 24 h. (A, C, E and G) Total RNA was prepared and subjected to RT-PCR analyses of DEC1 or DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. (B, D, F, and H) Protein was prepared from the cells and subjected to western blot analyses of DEC1 or DEC2, pSmad2, E-cadherin, N-cadherin, vimentin and actin. One representative result of at least three independent experiments with similar results is shown. DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2; EMT, epithelial-mesenchymal transition; RT-PCR, reverse transcription-polymerase chain reaction.

    Article Snippet: Cells were incubated with various concentrations of recombinant human TGF-β (R & D Systems, Inc., Minneapolis, MN, USA) for the indicated period of time.

    Techniques: Transfection, Expressing, Incubation, Reverse Transcription Polymerase Chain Reaction, Western Blot