human rhob gtpase Search Results


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  • 92
    Cell Signaling Technology Inc rho gtpase antibody sampler kit
    Cell-permeable PGDHC upregulates expression of DC-STAMP, an important osteoclast fusogen, in a Rac1-dependent manner A) Expression levels of three major <t>GTPases,</t> Rac1, RhoA, and Cdc42, by RANKL-stimulated RAW264.7 cells in response to ceramides and dihydroceramides: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, host cell-permeable C6-ceramide (hC6-C), or non-cell-permeable host C6-dihydroceramide (hC6-DHC) for 3 days, followed by the analysis of each <t>GTPase</t> expression using Western blot (left panel). The band intensity of each GTPase detected by W-blot was monitored using a densitometry, and expressed in the histograms (right graphs). B) PGDHC and hC6-C significantly upregulated DC-STAMP mRNA expression in RANKL-stimulated RAW 264.7 cells via a Rac1-depentent pathway: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, hC6-C, or hC6-DHC with or without Rac1 inhibitor (NSC23766) for 3 days. Expressions of DC-STAMP mRNA were evaluated by qPCR. Data are expressed as mean ± SD, and representative of three independent experiments is shown. * p
    Rho Gtpase Antibody Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rho gtpase antibody sampler kit/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rho gtpase antibody sampler kit - by Bioz Stars, 2021-05
    92/100 stars
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    97
    TaKaRa bspei
    Cell-permeable PGDHC upregulates expression of DC-STAMP, an important osteoclast fusogen, in a Rac1-dependent manner A) Expression levels of three major <t>GTPases,</t> Rac1, RhoA, and Cdc42, by RANKL-stimulated RAW264.7 cells in response to ceramides and dihydroceramides: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, host cell-permeable C6-ceramide (hC6-C), or non-cell-permeable host C6-dihydroceramide (hC6-DHC) for 3 days, followed by the analysis of each <t>GTPase</t> expression using Western blot (left panel). The band intensity of each GTPase detected by W-blot was monitored using a densitometry, and expressed in the histograms (right graphs). B) PGDHC and hC6-C significantly upregulated DC-STAMP mRNA expression in RANKL-stimulated RAW 264.7 cells via a Rac1-depentent pathway: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, hC6-C, or hC6-DHC with or without Rac1 inhibitor (NSC23766) for 3 days. Expressions of DC-STAMP mRNA were evaluated by qPCR. Data are expressed as mean ± SD, and representative of three independent experiments is shown. * p
    Bspei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bspei/product/TaKaRa
    Average 97 stars, based on 1 article reviews
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    bspei - by Bioz Stars, 2021-05
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    86
    Becton Dickinson human rhob gtpase
    Cell-permeable PGDHC upregulates expression of DC-STAMP, an important osteoclast fusogen, in a Rac1-dependent manner A) Expression levels of three major <t>GTPases,</t> Rac1, RhoA, and Cdc42, by RANKL-stimulated RAW264.7 cells in response to ceramides and dihydroceramides: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, host cell-permeable C6-ceramide (hC6-C), or non-cell-permeable host C6-dihydroceramide (hC6-DHC) for 3 days, followed by the analysis of each <t>GTPase</t> expression using Western blot (left panel). The band intensity of each GTPase detected by W-blot was monitored using a densitometry, and expressed in the histograms (right graphs). B) PGDHC and hC6-C significantly upregulated DC-STAMP mRNA expression in RANKL-stimulated RAW 264.7 cells via a Rac1-depentent pathway: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, hC6-C, or hC6-DHC with or without Rac1 inhibitor (NSC23766) for 3 days. Expressions of DC-STAMP mRNA were evaluated by qPCR. Data are expressed as mean ± SD, and representative of three independent experiments is shown. * p
    Human Rhob Gtpase, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human rhob gtpase/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human rhob gtpase - by Bioz Stars, 2021-05
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    Image Search Results


    Cell-permeable PGDHC upregulates expression of DC-STAMP, an important osteoclast fusogen, in a Rac1-dependent manner A) Expression levels of three major GTPases, Rac1, RhoA, and Cdc42, by RANKL-stimulated RAW264.7 cells in response to ceramides and dihydroceramides: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, host cell-permeable C6-ceramide (hC6-C), or non-cell-permeable host C6-dihydroceramide (hC6-DHC) for 3 days, followed by the analysis of each GTPase expression using Western blot (left panel). The band intensity of each GTPase detected by W-blot was monitored using a densitometry, and expressed in the histograms (right graphs). B) PGDHC and hC6-C significantly upregulated DC-STAMP mRNA expression in RANKL-stimulated RAW 264.7 cells via a Rac1-depentent pathway: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, hC6-C, or hC6-DHC with or without Rac1 inhibitor (NSC23766) for 3 days. Expressions of DC-STAMP mRNA were evaluated by qPCR. Data are expressed as mean ± SD, and representative of three independent experiments is shown. * p

    Journal: Biochimica et biophysica acta

    Article Title: Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor

    doi: 10.1016/j.bbalip.2017.01.008

    Figure Lengend Snippet: Cell-permeable PGDHC upregulates expression of DC-STAMP, an important osteoclast fusogen, in a Rac1-dependent manner A) Expression levels of three major GTPases, Rac1, RhoA, and Cdc42, by RANKL-stimulated RAW264.7 cells in response to ceramides and dihydroceramides: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, host cell-permeable C6-ceramide (hC6-C), or non-cell-permeable host C6-dihydroceramide (hC6-DHC) for 3 days, followed by the analysis of each GTPase expression using Western blot (left panel). The band intensity of each GTPase detected by W-blot was monitored using a densitometry, and expressed in the histograms (right graphs). B) PGDHC and hC6-C significantly upregulated DC-STAMP mRNA expression in RANKL-stimulated RAW 264.7 cells via a Rac1-depentent pathway: RANKL-stimulated RAW264.7 cells were incubated in the presence or absence of PGDHC, hC6-C, or hC6-DHC with or without Rac1 inhibitor (NSC23766) for 3 days. Expressions of DC-STAMP mRNA were evaluated by qPCR. Data are expressed as mean ± SD, and representative of three independent experiments is shown. * p

    Article Snippet: Small GTPases, including Rac1, RhoA and Cdc42, were identified using a Rho-GTPase antibody sampler kit (Cell Signaling Technology, Danvers, MA).

    Techniques: Expressing, Incubation, Western Blot, Real-time Polymerase Chain Reaction

    Knock-out of GPRC5A modulates the activity of RhoA and Rac1 GTPases. (A) Western blot demonstrating the amount of RhoA, Rac1, and cdc42 GTPases immunoprecipitated in GTP-bound state from the cells collected 30 minutes after plating on Collagen I (0.1 mg/mL). Three control (C1-3) and 3 knock-out (K1-3) samples were collected independently (N = 3). (B) Quantification of the experiment shown in (A). The signal from precipitated GTP-bound GTPases was normalized to the total amount of a corresponding GTPase protein and the loading control, and presented as % of control. Bars represent mean ± SEM, N = 3. Statistical significance was evaluated using one-way ANOVA test. *, p

    Journal: Cell Adhesion & Migration

    Article Title: Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion

    doi: 10.1080/19336918.2016.1245264

    Figure Lengend Snippet: Knock-out of GPRC5A modulates the activity of RhoA and Rac1 GTPases. (A) Western blot demonstrating the amount of RhoA, Rac1, and cdc42 GTPases immunoprecipitated in GTP-bound state from the cells collected 30 minutes after plating on Collagen I (0.1 mg/mL). Three control (C1-3) and 3 knock-out (K1-3) samples were collected independently (N = 3). (B) Quantification of the experiment shown in (A). The signal from precipitated GTP-bound GTPases was normalized to the total amount of a corresponding GTPase protein and the loading control, and presented as % of control. Bars represent mean ± SEM, N = 3. Statistical significance was evaluated using one-way ANOVA test. *, p

    Article Snippet: The following primary antibodies have been used: rabbit anti-integrin β1 (#EP1041Y, Abcam, diluted 1:2500); rabbit anti-GPRC5A (#HPA007928, Sigma Aldrich, diluted 1:1000); rabbit anti-FAK antibody sampler kit (#9330, Cell Signaling Technologies, 1:1000); rabbit anti-Rho GTPases antibody sampler kit (#9968, Cell Signaling Technologies, 1:1000); mouse anti-β actin (A2228, Sigma-Aldrich, 1:2000); goat anti-EphA2 (AF3035, R & D Systems, 1:2000); and mouse anti-GAPDH (#NB300-328, Novus Biologicals, 1:5000).

    Techniques: Knock-Out, Activity Assay, Western Blot, Immunoprecipitation